Moc05g14710 (gene) Bitter gourd (OHB3-1) v2

Overview


Name	Moc05g14710
Type	gene
Organism	Momordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
Description	Ribonuclease H
Location	chr5: 11094962 .. 11095690 (+)
RNA-Seq Expression	Moc05g14710
Synteny	Moc05g14710

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCACTCAGCGAGCTGGACGCCAAAGTGACCGAAGATCAGGCCAGAGTAGGACAGGAAGTGGAGCCAGCTCGGAGGTCCACCCGCAATGCCAATATCGTCCTGCCTCCGGTACATCCGAGGCCTAGGAAGACCAACCGAGGTCGAGGTGAGACCTCGAAAAAGATCTCACGAAGGGCCGCGCCTGCTGTCGACCCTGAAGTTCTCGCCACCATTCAACGGGAGTTAGACGATATGCGCAACCGAGTGCGCACCATGGAGGAAACGTACAATGAGATGATGAGGGCAAACCGAACTGGGTCCCATTTCAAGGCCTCAGAAGGGACGGACGACCTGTCGAAAGATGGAGATGACTTGGATCCACCCATCTACCCCGATGACTATGAGCCGGTTGCCGATAACGAAGGGATCAATTATAGTTTGAGGGATCATGACCTTCGGAAGCACCTCGTCGATAAGAAGATGAAGGCCTCACGGGGCCCAGGGATTTCATCATCCTGCTCTAAAAAATATTCCAACTCCGACCTCAAGGCCCAGTCAAAGTACACGCTATTAACGCCAGAGGCCGTGATTACGAGGGAAGAGTTCAACCTGAAGAAACACAGATTCAACGAACAAGTCGAGGCACTGAAGGCTAACTGCAAAAAGAAAGAAAGCCAGTTTGATGATGGCGAGATTGGTGAATCGCCATTCACCTCTGACGTATTGGAGGCCCCAATCCCTCCATAG

mRNA sequence

ATGGCACTCAGCGAGCTGGACGCCAAAGTGACCGAAGATCAGGCCAGAGTAGGACAGGAAGTGGAGCCAGCTCGGAGGTCCACCCGCAATGCCAATATCGTCCTGCCTCCGGTACATCCGAGGCCTAGGAAGACCAACCGAGGTCGAGGTGAGACCTCGAAAAAGATCTCACGAAGGGCCGCGCCTGCTGTCGACCCTGAAGTTCTCGCCACCATTCAACGGGAGTTAGACGATATGCGCAACCGAGTGCGCACCATGGAGGAAACGTACAATGAGATGATGAGGGCAAACCGAACTGGGTCCCATTTCAAGGCCTCAGAAGGGACGGACGACCTGTCGAAAGATGGAGATGACTTGGATCCACCCATCTACCCCGATGACTATGAGCCGGTTGCCGATAACGAAGGGATCAATTATAGTTTGAGGGATCATGACCTTCGGAAGCACCTCGTCGATAAGAAGATGAAGGCCTCACGGGGCCCAGGGATTTCATCATCCTGCTCTAAAAAATATTCCAACTCCGACCTCAAGGCCCAGTCAAAGTACACGCTATTAACGCCAGAGGCCGTGATTACGAGGGAAGAGTTCAACCTGAAGAAACACAGATTCAACGAACAAGTCGAGGCACTGAAGGCTAACTGCAAAAAGAAAGAAAGCCAGTTTGATGATGGCGAGATTGGTGAATCGCCATTCACCTCTGACGTATTGGAGGCCCCAATCCCTCCATAG

Coding sequence (CDS)

ATGGCACTCAGCGAGCTGGACGCCAAAGTGACCGAAGATCAGGCCAGAGTAGGACAGGAAGTGGAGCCAGCTCGGAGGTCCACCCGCAATGCCAATATCGTCCTGCCTCCGGTACATCCGAGGCCTAGGAAGACCAACCGAGGTCGAGGTGAGACCTCGAAAAAGATCTCACGAAGGGCCGCGCCTGCTGTCGACCCTGAAGTTCTCGCCACCATTCAACGGGAGTTAGACGATATGCGCAACCGAGTGCGCACCATGGAGGAAACGTACAATGAGATGATGAGGGCAAACCGAACTGGGTCCCATTTCAAGGCCTCAGAAGGGACGGACGACCTGTCGAAAGATGGAGATGACTTGGATCCACCCATCTACCCCGATGACTATGAGCCGGTTGCCGATAACGAAGGGATCAATTATAGTTTGAGGGATCATGACCTTCGGAAGCACCTCGTCGATAAGAAGATGAAGGCCTCACGGGGCCCAGGGATTTCATCATCCTGCTCTAAAAAATATTCCAACTCCGACCTCAAGGCCCAGTCAAAGTACACGCTATTAACGCCAGAGGCCGTGATTACGAGGGAAGAGTTCAACCTGAAGAAACACAGATTCAACGAACAAGTCGAGGCACTGAAGGCTAACTGCAAAAAGAAAGAAAGCCAGTTTGATGATGGCGAGATTGGTGAATCGCCATTCACCTCTGACGTATTGGAGGCCCCAATCCCTCCATAG

Protein sequence

MALSELDAKVTEDQARVGQEVEPARRSTRNANIVLPPVHPRPRKTNRGRGETSKKISRRAAPAVDPEVLATIQRELDDMRNRVRTMEETYNEMMRANRTGSHFKASEGTDDLSKDGDDLDPPIYPDDYEPVADNEGINYSLRDHDLRKHLVDKKMKASRGPGISSSCSKKYSNSDLKAQSKYTLLTPEAVITREEFNLKKHRFNEQVEALKANCKKKESQFDDGEIGESPFTSDVLEAPIPP

Homology

[Close]

BLAST of Moc05g14710 vs. NCBI nr
Match: XP_022154406.1 (uncharacterized protein LOC111021683 [Momordica charantia])

HSP 1 Score: 168.7 bits (426), Expect = 6.2e-38
Identity = 92/157 (58.60%), Postives = 114/157 (72.61%), Query Frame = 0


Query: 86  MEETYNEMMRANRTGSHFKASEGTDDLSKDGDDLDPPIYPDDYEPVADNEGINYSLRDHD 145
           ME+ Y E  RANRT S   A          G+   P I P D EP+ ++ G++YSLRD+D
Sbjct: 1   MEKMYAEATRANRTASPSIAP------GAPGEKGAPSIQPGDREPIPNDGGMDYSLRDND 60

Query: 146 LRKHLVDKKMKASRGPGISSSCSKKYSNSDLKAQSKYTLLTPEAVITREEFNLKKHRFNE 205
           +RKHL +KK +ASR P  S S S+K+SNS+LKAQSKY  L PEA+ITREEF+L KH+F E
Sbjct: 61  MRKHLTEKKKRASREPEDSLSYSRKFSNSNLKAQSKYKPLAPEAMITREEFDLMKHKFGE 120

Query: 206 QVEALKANCKKKESQFDDGEIGESPFTSDVLEAPIPP 243
           QVEALKA C+KKE  FD+G++GESPFT D+LEAPIPP
Sbjct: 121 QVEALKARCEKKECSFDNGDLGESPFTPDILEAPIPP 151

[Close]

BLAST of Moc05g14710 vs. NCBI nr
Match: XP_022155139.1 (uncharacterized protein LOC111022280 [Momordica charantia])

HSP 1 Score: 163.7 bits (413), Expect = 2.0e-36
Identity = 84/127 (66.14%), Postives = 103/127 (81.10%), Query Frame = 0


Query: 116 GDDLDPPIYPDDYEPVADNEGINYSLRDHDLRKHLVDKKMKASRGPGISSSCSKKYSNSD 175
           G+   P I P + EP+ ++EG++YSLRD+DLRKHL DKK KAS  P  S S S+++SNS+
Sbjct: 9   GEKGAPSIQPGNREPIPNDEGVDYSLRDNDLRKHLTDKKKKASWEPEDSLSYSREFSNSN 68

Query: 176 LKAQSKYTLLTPEAVITREEFNLKKHRFNEQVEALKANCKKKESQFDDGEIGESPFTSDV 235
           LKAQSKY  L PEAVI REEF+L KHRF+EQVEALKA C+KKES FDD ++GESPFTSD+
Sbjct: 69  LKAQSKYKPLIPEAVINREEFDLMKHRFDEQVEALKARCEKKESPFDDDDLGESPFTSDI 128

Query: 236 LEAPIPP 243
           +EAPIPP
Sbjct: 129 MEAPIPP 135

[Close]

BLAST of Moc05g14710 vs. NCBI nr
Match: XP_022159327.1 (uncharacterized protein LOC111025738 [Momordica charantia])

HSP 1 Score: 146.7 bits (369), Expect = 2.5e-31
Identity = 99/237 (41.77%), Postives = 128/237 (54.01%), Query Frame = 0


Query: 5   ELDAKVTEDQARVGQEVEPARRSTRNANIVLPPVHPRPRKTNRGRGETSKKISRRAAPAV 64
           E+ A+V E Q   G   EP  RS R     L P HP+P K NRGRG  S++ +  AAPA 
Sbjct: 25  EVGAEVVEGQIHEGLGTEPFCRSARITTPDLSPAHPKPFKANRGRGGASRRTTLGAAPAP 84

Query: 65  DPEVLATIQRELDDMRNRVRTMEETYNEMMRANRTGSHFKASEGTDDLSKDGDDLDPPIY 124
             E    +Q+E++ MR ++ TMEE YNEM++A   GS  +     D+             
Sbjct: 85  SRENFDALQKEMEAMRTQMLTMEEMYNEMVQAVGAGSRSEDRAARDERG----------- 144

Query: 125 PDDYEPVADNEGINYSLRDHDLRKHLVDKKMKASRGPGISSSCSKKYSNSDLKAQSKYTL 184
                               DLR HL  +K  +S   G S SCS K  NS+ +A+S Y  
Sbjct: 145 --------------------DLRDHL-SRKRSSSLRKGRSPSCSHK--NSNQQAESSYNP 204

Query: 185 LTPEAVITREEFNLKKHRFNEQVEALKANCKKKESQFDDGEIGESPFTSDVLEAPIP 242
           + PE VITREEF+  K +F+ QVE LKA C+ K S FDDG++GESPFTSD+LEA IP
Sbjct: 205 VVPEGVITREEFDQLKSKFDAQVETLKARCEVKGSTFDDGDLGESPFTSDILEALIP 227

[Close]

BLAST of Moc05g14710 vs. NCBI nr
Match: XP_022157897.1 (uncharacterized protein LOC111024503 [Momordica charantia])

HSP 1 Score: 124.4 bits (311), Expect = 1.3e-24
Identity = 63/87 (72.41%), Postives = 73/87 (83.91%), Query Frame = 0


Query: 156 KASRGPGISSSCSKKYSNSDLKAQSKYTLLTPEAVITREEFNLKKHRFNEQVEALKANCK 215
           +ASR    S S S+++SNSD KAQSKY  LTPEAVITREEF+L KHRF+EQVEALKA C+
Sbjct: 468 RASRESEDSPSYSREFSNSDFKAQSKYKPLTPEAVITREEFDLMKHRFDEQVEALKAKCE 527

Query: 216 KKESQFDDGEIGESPFTSDVLEAPIPP 243
           KKE  FDDG++GESPFTSD+LE PIPP
Sbjct: 528 KKECSFDDGDLGESPFTSDILEVPIPP 554

[Close]

BLAST of Moc05g14710 vs. NCBI nr
Match: XP_022156638.1 (uncharacterized protein LOC111023488 [Momordica charantia])

HSP 1 Score: 120.9 bits (302), Expect = 1.5e-23
Identity = 70/149 (46.98%), Postives = 79/149 (53.02%), Query Frame = 0


Query: 2   ALSELDAKVTEDQARVGQEVEPARRSTRNANIVLPPVHPRPRKTNRGRGETSKKISRRAA 61
           A  ELDAKVTE+Q + GQEV+P RRSTRNAN +LPP HPR  K NRGRG           
Sbjct: 22  AQHELDAKVTEEQTKAGQEVDPPRRSTRNANTILPPAHPRSGKANRGRG----------- 81

Query: 62  PAVDPEVLATIQRELDDMRNRVRTMEETYNEMMRANRTGSHFKASEGTDDLSKDGDDLDP 121
                                                         GTDDL +DGDDLDP
Sbjct: 82  ---------------------------------------------GGTDDLPEDGDDLDP 114

Query: 122 PIYPDDYEPVADNEGINYSLRDHDLRKHL 151
           PI+PDDYEP  +N+GI+YSLRDHDLRKHL
Sbjct: 142 PIHPDDYEPAVENKGIDYSLRDHDLRKHL 114

[Close]

BLAST of Moc05g14710 vs. ExPASy TrEMBL
Match: A0A6J1DNM4 (Ribonuclease H OS=Momordica charantia OX=3673 GN=LOC111021683 PE=4 SV=1)

HSP 1 Score: 168.7 bits (426), Expect = 3.0e-38
Identity = 92/157 (58.60%), Postives = 114/157 (72.61%), Query Frame = 0


Query: 86  MEETYNEMMRANRTGSHFKASEGTDDLSKDGDDLDPPIYPDDYEPVADNEGINYSLRDHD 145
           ME+ Y E  RANRT S   A          G+   P I P D EP+ ++ G++YSLRD+D
Sbjct: 1   MEKMYAEATRANRTASPSIAP------GAPGEKGAPSIQPGDREPIPNDGGMDYSLRDND 60

Query: 146 LRKHLVDKKMKASRGPGISSSCSKKYSNSDLKAQSKYTLLTPEAVITREEFNLKKHRFNE 205
           +RKHL +KK +ASR P  S S S+K+SNS+LKAQSKY  L PEA+ITREEF+L KH+F E
Sbjct: 61  MRKHLTEKKKRASREPEDSLSYSRKFSNSNLKAQSKYKPLAPEAMITREEFDLMKHKFGE 120

Query: 206 QVEALKANCKKKESQFDDGEIGESPFTSDVLEAPIPP 243
           QVEALKA C+KKE  FD+G++GESPFT D+LEAPIPP
Sbjct: 121 QVEALKARCEKKECSFDNGDLGESPFTPDILEAPIPP 151

[Close]

BLAST of Moc05g14710 vs. ExPASy TrEMBL
Match: A0A6J1DPC9 (uncharacterized protein LOC111022280 OS=Momordica charantia OX=3673 GN=LOC111022280 PE=4 SV=1)

HSP 1 Score: 163.7 bits (413), Expect = 9.6e-37
Identity = 84/127 (66.14%), Postives = 103/127 (81.10%), Query Frame = 0


Query: 116 GDDLDPPIYPDDYEPVADNEGINYSLRDHDLRKHLVDKKMKASRGPGISSSCSKKYSNSD 175
           G+   P I P + EP+ ++EG++YSLRD+DLRKHL DKK KAS  P  S S S+++SNS+
Sbjct: 9   GEKGAPSIQPGNREPIPNDEGVDYSLRDNDLRKHLTDKKKKASWEPEDSLSYSREFSNSN 68

Query: 176 LKAQSKYTLLTPEAVITREEFNLKKHRFNEQVEALKANCKKKESQFDDGEIGESPFTSDV 235
           LKAQSKY  L PEAVI REEF+L KHRF+EQVEALKA C+KKES FDD ++GESPFTSD+
Sbjct: 69  LKAQSKYKPLIPEAVINREEFDLMKHRFDEQVEALKARCEKKESPFDDDDLGESPFTSDI 128

Query: 236 LEAPIPP 243
           +EAPIPP
Sbjct: 129 MEAPIPP 135

[Close]

BLAST of Moc05g14710 vs. ExPASy TrEMBL
Match: A0A6J1DZJ1 (uncharacterized protein LOC111025738 OS=Momordica charantia OX=3673 GN=LOC111025738 PE=4 SV=1)

HSP 1 Score: 146.7 bits (369), Expect = 1.2e-31
Identity = 99/237 (41.77%), Postives = 128/237 (54.01%), Query Frame = 0


Query: 5   ELDAKVTEDQARVGQEVEPARRSTRNANIVLPPVHPRPRKTNRGRGETSKKISRRAAPAV 64
           E+ A+V E Q   G   EP  RS R     L P HP+P K NRGRG  S++ +  AAPA 
Sbjct: 25  EVGAEVVEGQIHEGLGTEPFCRSARITTPDLSPAHPKPFKANRGRGGASRRTTLGAAPAP 84

Query: 65  DPEVLATIQRELDDMRNRVRTMEETYNEMMRANRTGSHFKASEGTDDLSKDGDDLDPPIY 124
             E    +Q+E++ MR ++ TMEE YNEM++A   GS  +     D+             
Sbjct: 85  SRENFDALQKEMEAMRTQMLTMEEMYNEMVQAVGAGSRSEDRAARDERG----------- 144

Query: 125 PDDYEPVADNEGINYSLRDHDLRKHLVDKKMKASRGPGISSSCSKKYSNSDLKAQSKYTL 184
                               DLR HL  +K  +S   G S SCS K  NS+ +A+S Y  
Sbjct: 145 --------------------DLRDHL-SRKRSSSLRKGRSPSCSHK--NSNQQAESSYNP 204

Query: 185 LTPEAVITREEFNLKKHRFNEQVEALKANCKKKESQFDDGEIGESPFTSDVLEAPIP 242
           + PE VITREEF+  K +F+ QVE LKA C+ K S FDDG++GESPFTSD+LEA IP
Sbjct: 205 VVPEGVITREEFDQLKSKFDAQVETLKARCEVKGSTFDDGDLGESPFTSDILEALIP 227

[Close]

BLAST of Moc05g14710 vs. ExPASy TrEMBL
Match: A0A6J1DVR0 (uncharacterized protein LOC111024503 OS=Momordica charantia OX=3673 GN=LOC111024503 PE=4 SV=1)

HSP 1 Score: 124.4 bits (311), Expect = 6.5e-25
Identity = 63/87 (72.41%), Postives = 73/87 (83.91%), Query Frame = 0


Query: 156 KASRGPGISSSCSKKYSNSDLKAQSKYTLLTPEAVITREEFNLKKHRFNEQVEALKANCK 215
           +ASR    S S S+++SNSD KAQSKY  LTPEAVITREEF+L KHRF+EQVEALKA C+
Sbjct: 468 RASRESEDSPSYSREFSNSDFKAQSKYKPLTPEAVITREEFDLMKHRFDEQVEALKAKCE 527

Query: 216 KKESQFDDGEIGESPFTSDVLEAPIPP 243
           KKE  FDDG++GESPFTSD+LE PIPP
Sbjct: 528 KKECSFDDGDLGESPFTSDILEVPIPP 554

[Close]

BLAST of Moc05g14710 vs. ExPASy TrEMBL
Match: A0A6J1DR60 (uncharacterized protein LOC111023488 OS=Momordica charantia OX=3673 GN=LOC111023488 PE=4 SV=1)

HSP 1 Score: 120.9 bits (302), Expect = 7.2e-24
Identity = 70/149 (46.98%), Postives = 79/149 (53.02%), Query Frame = 0


Query: 2   ALSELDAKVTEDQARVGQEVEPARRSTRNANIVLPPVHPRPRKTNRGRGETSKKISRRAA 61
           A  ELDAKVTE+Q + GQEV+P RRSTRNAN +LPP HPR  K NRGRG           
Sbjct: 22  AQHELDAKVTEEQTKAGQEVDPPRRSTRNANTILPPAHPRSGKANRGRG----------- 81

Query: 62  PAVDPEVLATIQRELDDMRNRVRTMEETYNEMMRANRTGSHFKASEGTDDLSKDGDDLDP 121
                                                         GTDDL +DGDDLDP
Sbjct: 82  ---------------------------------------------GGTDDLPEDGDDLDP 114

Query: 122 PIYPDDYEPVADNEGINYSLRDHDLRKHL 151
           PI+PDDYEP  +N+GI+YSLRDHDLRKHL
Sbjct: 142 PIHPDDYEPAVENKGIDYSLRDHDLRKHL 114

The following BLAST results are available for this feature:

BLAST of Moc05g14710 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022154406.1	6.2e-38	58.60	uncharacterized protein LOC111021683 [Momordica charantia]	[more]
XP_022155139.1	2.0e-36	66.14	uncharacterized protein LOC111022280 [Momordica charantia]	[more]
XP_022159327.1	2.5e-31	41.77	uncharacterized protein LOC111025738 [Momordica charantia]	[more]
XP_022157897.1	1.3e-24	72.41	uncharacterized protein LOC111024503 [Momordica charantia]	[more]
XP_022156638.1	1.5e-23	46.98	uncharacterized protein LOC111023488 [Momordica charantia]	[more]

BLAST of Moc05g14710 vs. NCBI nr


Match Name	E-value	Identity	Description

BLAST of Moc05g14710 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1DNM4	3.0e-38	58.60	Ribonuclease H OS=Momordica charantia OX=3673 GN=LOC111021683 PE=4 SV=1	[more]
A0A6J1DPC9	9.6e-37	66.14	uncharacterized protein LOC111022280 OS=Momordica charantia OX=3673 GN=LOC111022...	[more]
A0A6J1DZJ1	1.2e-31	41.77	uncharacterized protein LOC111025738 OS=Momordica charantia OX=3673 GN=LOC111025...	[more]
A0A6J1DVR0	6.5e-25	72.41	uncharacterized protein LOC111024503 OS=Momordica charantia OX=3673 GN=LOC111024...	[more]
A0A6J1DR60	7.2e-24	46.98	uncharacterized protein LOC111023488 OS=Momordica charantia OX=3673 GN=LOC111023...	[more]

BLAST of Moc05g14710 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (OHB3-1) v2
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	COILS	Coil	Coil	coord: 69..96
None	No IPR available	COILS	Coil	Coil	coord: 193..220
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 218..242
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 104..121
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 98..121
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 44..61
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 9..23
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 1..69

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Moc05g14710.1	Moc05g14710.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0090304	nucleic acid metabolic process
cellular_component	GO:0016021	integral component of membrane