Moc05g14660 (gene) Bitter gourd (OHB3-1) v2

Overview


Name	Moc05g14660
Type	gene
Organism	Momordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
Description	Reverse transcriptase
Location	chr5: 11073828 .. 11074205 (-)
RNA-Seq Expression	Moc05g14660
Synteny	Moc05g14660

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAAATTACCAGGAGACTTTGAAGAGTGCTCTGCTATAACTAGCTTGAATCCTGTTATCTTTGATGAGTTTTATGACTTGTTAGTTACAGATATTGAAGAAGAGCTTGATAAGATAGCAGAACGACCGGAAGATGTGACTAATCCTGTTGAAAAAATACAAAAAGAAGAATGCAAGTCGTTACTTCCGTCCATAGTGGAACCACCCACTTTGGAGCAGAAGTCATTGTCGTCGCATTTGAAATATGCGTATCTAGGGGATAACGACACTTTACCAGTTATTATAGCTTCCAATTTATCACCTACTAATGAATATTCTTTATTGCAGATTTTGGAGAAGCACAAAAAGTCCATTGGATGGACGATAACAGATATCTGA

mRNA sequence

ATGAAATTACCAGGAGACTTTGAAGAGTGCTCTGCTATAACTAGCTTGAATCCTGTTATCTTTGATGAGTTTTATGACTTGTTAGTTACAGATATTGAAGAAGAGCTTGATAAGATAGCAGAACGACCGGAAGATGTGACTAATCCTGTTGAAAAAATACAAAAAGAAGAATGCAAGTCGTTACTTCCGTCCATAGTGGAACCACCCACTTTGGAGCAGAAGTCATTGTCGTCGCATTTGAAATATGCGTATCTAGGGGATAACGACACTTTACCAGTTATTATAGCTTCCAATTTATCACCTACTAATGAATATTCTTTATTGCAGATTTTGGAGAAGCACAAAAAGTCCATTGGATGGACGATAACAGATATCTGA

Coding sequence (CDS)

ATGAAATTACCAGGAGACTTTGAAGAGTGCTCTGCTATAACTAGCTTGAATCCTGTTATCTTTGATGAGTTTTATGACTTGTTAGTTACAGATATTGAAGAAGAGCTTGATAAGATAGCAGAACGACCGGAAGATGTGACTAATCCTGTTGAAAAAATACAAAAAGAAGAATGCAAGTCGTTACTTCCGTCCATAGTGGAACCACCCACTTTGGAGCAGAAGTCATTGTCGTCGCATTTGAAATATGCGTATCTAGGGGATAACGACACTTTACCAGTTATTATAGCTTCCAATTTATCACCTACTAATGAATATTCTTTATTGCAGATTTTGGAGAAGCACAAAAAGTCCATTGGATGGACGATAACAGATATCTGA

Protein sequence

MKLPGDFEECSAITSLNPVIFDEFYDLLVTDIEEELDKIAERPEDVTNPVEKIQKEECKSLLPSIVEPPTLEQKSLSSHLKYAYLGDNDTLPVIIASNLSPTNEYSLLQILEKHKKSIGWTITDI

Homology

[Close]

BLAST of Moc05g14660 vs. NCBI nr
Match: XP_022151603.1 (LOW QUALITY PROTEIN: uncharacterized protein LOC111019515 [Momordica charantia])

HSP 1 Score: 173.3 bits (438), Expect = 1.3e-39
Identity = 86/95 (90.53%), Postives = 90/95 (94.74%), Query Frame = 0


Query: 31  DIEEELDKIAERPEDVTNPVEKIQKEECKSLLPSIVEPPTLEQKSLSSHLKYAYLGDNDT 90
           +IEEELDKIAE PEDVT+PVEKIQKEECKSLLPSIVEPPTLEQK L SHLKYAYLGDNDT
Sbjct: 35  EIEEELDKIAEGPEDVTDPVEKIQKEECKSLLPSIVEPPTLEQKPLPSHLKYAYLGDNDT 94

Query: 91  LPVIIASNLSPTNEYSLLQILEKHKKSIGWTITDI 126
           LPVI+ SNLSPTNEYSLLQILEKHKK+IGWTI DI
Sbjct: 95  LPVIJXSNLSPTNEYSLLQILEKHKKAIGWTIADI 129

[Close]

BLAST of Moc05g14660 vs. NCBI nr
Match: XP_022158979.1 (uncharacterized protein LOC111025424 [Momordica charantia])

HSP 1 Score: 120.2 bits (300), Expect = 1.3e-23
Identity = 57/63 (90.48%), Postives = 60/63 (95.24%), Query Frame = 0


Query: 63  PSIVEPPTLEQKSLSSHLKYAYLGDNDTLPVIIASNLSPTNEYSLLQILEKHKKSIGWTI 122
           P+IVEPPTLEQK L SHLKYAYLGDN+TLPVIIASNLSPTNEYSLLQILEKHKK+IGWTI
Sbjct: 204 PTIVEPPTLEQKPLPSHLKYAYLGDNBTLPVIIASNLSPTNEYSLLQILEKHKKAIGWTI 263

Query: 123 TDI 126
            DI
Sbjct: 264 ADI 266

[Close]

BLAST of Moc05g14660 vs. NCBI nr
Match: XP_030502183.1 (uncharacterized protein LOC115717351 [Cannabis sativa])

HSP 1 Score: 105.1 bits (261), Expect = 4.3e-19
Identity = 61/145 (42.07%), Postives = 86/145 (59.31%), Query Frame = 0


Query: 1   MKLPGDFEECSAITSLNPVIFDEFYDLLVTDIE--EELDKIAERPEDVTN---------- 60
           M+ P + EECS I+ ++ ++ ++F+     D +     D++ +  ED  N          
Sbjct: 275 MRFPDEIEECSRISVIDSIVAEKFHKEACKDEKFISSFDELEDLSEDEDNQVAWVEPLQP 334

Query: 61  ------PVEKIQKEEC--KSLLPSIVEPPTLEQKSLSSHLKYAYLGDNDTLPVIIASNLS 120
                 P E ++ +E   K   PS  EPP LE K L SHLKYAYLG+NDTLPVIIASNL 
Sbjct: 335 MPKFKKPFESLELKESNFKPPKPSTQEPPKLELKPLPSHLKYAYLGENDTLPVIIASNLV 394

Query: 121 PTNEYSLLQILEKHKKSIGWTITDI 126
             +E +LL++L+KHKK+IGWT+ DI
Sbjct: 395 VEDEGALLEVLKKHKKAIGWTMADI 419

[Close]

BLAST of Moc05g14660 vs. NCBI nr
Match: XP_030504461.1 (uncharacterized protein LOC115719521 [Cannabis sativa])

HSP 1 Score: 100.5 bits (249), Expect = 1.1e-17
Identity = 58/145 (40.00%), Postives = 85/145 (58.62%), Query Frame = 0


Query: 1   MKLPGDFEECSAITSLNPVIFDEFY-----DLLVTDIEEELDKIAERPEDVTNPVEKIQ- 60
           M+ P + EECS ++ ++ ++ + F+     D  V    E+L+ ++E  E   + VE +Q 
Sbjct: 142 MRFPDEIEECSRLSVIDSIVAESFHKKVWKDERVISSLEDLEALSEDEETQVSWVEPMQP 201

Query: 61  --------------KEECKSLLPSIVEPPTLEQKSLSSHLKYAYLGDNDTLPVIIASNLS 120
                         +   K L PSI EPP LE K L SHLKYAYLG+N+TLPVII++ L 
Sbjct: 202 FPKFKRAFESLELKESNFKPLKPSIQEPPKLELKPLPSHLKYAYLGENETLPVIISAWLE 261

Query: 121 PTNEYSLLQILEKHKKSIGWTITDI 126
              E  LL++L+KHK++IGWT+ DI
Sbjct: 262 AETEGLLLEVLKKHKRAIGWTMADI 286

[Close]

BLAST of Moc05g14660 vs. NCBI nr
Match: XP_021745351.1 (uncharacterized protein LOC110711280 [Chenopodium quinoa])

HSP 1 Score: 99.8 bits (247), Expect = 1.8e-17
Identity = 52/100 (52.00%), Postives = 67/100 (67.00%), Query Frame = 0


Query: 28  LVTDIEEELDKIAERPEDVTNPVEKIQKEECKS--LLPSIVEPPTLEQKSLSSHLKYAYL 87
           L  D++E    +   P      V+K  K    S  LLPSIV+PP +E K L  HLKYA+L
Sbjct: 370 LENDVKEAFSSLEVLPNKQGRYVDKFMKFPVTSNRLLPSIVQPPEVELKLLPYHLKYAFL 429

Query: 88  GDNDTLPVIIASNLSPTNEYSLLQILEKHKKSIGWTITDI 126
           G+NDTLPVIIASNL+PT E  L+Q+L+KHK ++GWT+ DI
Sbjct: 430 GENDTLPVIIASNLTPTQEQKLVQVLKKHKTTLGWTVADI 469

[Close]

BLAST of Moc05g14660 vs. ExPASy TrEMBL
Match: A0A6J1DCL7 (LOW QUALITY PROTEIN: uncharacterized protein LOC111019515 OS=Momordica charantia OX=3673 GN=LOC111019515 PE=4 SV=1)

HSP 1 Score: 172.9 bits (437), Expect = 8.2e-40
Identity = 86/95 (90.53%), Postives = 90/95 (94.74%), Query Frame = 0


Query: 31  DIEEELDKIAERPEDVTNPVEKIQKEECKSLLPSIVEPPTLEQKSLSSHLKYAYLGDNDT 90
           +IEEELDKIAE PEDVT+PVEKIQKEECKSLLPSIVEPPTLEQK L SHLKYAYLGDNDT
Sbjct: 35  EIEEELDKIAEGPEDVTDPVEKIQKEECKSLLPSIVEPPTLEQKPLPSHLKYAYLGDNDT 94

Query: 91  LPVIIASNLSPTNEYSLLQILEKHKKSIGWTITDI 126
           LPVI+ SNLSPTNEYSLLQILEKHKK+IGWTI DI
Sbjct: 95  LPVILXSNLSPTNEYSLLQILEKHKKAIGWTIADI 129

[Close]

BLAST of Moc05g14660 vs. ExPASy TrEMBL
Match: A0A6J1E110 (uncharacterized protein LOC111025424 OS=Momordica charantia OX=3673 GN=LOC111025424 PE=4 SV=1)

HSP 1 Score: 120.9 bits (302), Expect = 3.7e-24
Identity = 58/63 (92.06%), Postives = 60/63 (95.24%), Query Frame = 0


Query: 63  PSIVEPPTLEQKSLSSHLKYAYLGDNDTLPVIIASNLSPTNEYSLLQILEKHKKSIGWTI 122
           P+IVEPPTLEQK L SHLKYAYLGDNDTLPVIIASNLSPTNEYSLLQILEKHKK+IGWTI
Sbjct: 204 PTIVEPPTLEQKPLPSHLKYAYLGDNDTLPVIIASNLSPTNEYSLLQILEKHKKAIGWTI 263

Query: 123 TDI 126
            DI
Sbjct: 264 ADI 266

[Close]

BLAST of Moc05g14660 vs. ExPASy TrEMBL
Match: A0A6P6UH48 (uncharacterized protein LOC113711211 OS=Coffea arabica OX=13443 GN=LOC113711211 PE=4 SV=1)

HSP 1 Score: 93.6 bits (231), Expect = 6.3e-16
Identity = 43/75 (57.33%), Postives = 58/75 (77.33%), Query Frame = 0


Query: 51  EKIQKEECKSLLPSIVEPPTLEQKSLSSHLKYAYLGDNDTLPVIIASNLSPTNEYSLLQI 110
           E + ++E + +LPS+V+ P LE K L  HLKYAYLGDN+TLPVII+S LS T E  L+++
Sbjct: 248 ETVGRDELERVLPSVVQAPVLELKPLPEHLKYAYLGDNETLPVIISSALSKTQEEKLIRV 307

Query: 111 LEKHKKSIGWTITDI 126
           L +HK++IGWTI DI
Sbjct: 308 LREHKEAIGWTIADI 322

[Close]

BLAST of Moc05g14660 vs. ExPASy TrEMBL
Match: A0A6N2NBX1 (Uncharacterized protein OS=Salix viminalis OX=40686 GN=SVIM_LOCUS482486 PE=4 SV=1)

HSP 1 Score: 93.2 bits (230), Expect = 8.2e-16
Identity = 54/149 (36.24%), Postives = 83/149 (55.70%), Query Frame = 0


Query: 1   MKLPGDFEECSAITSLNPVIFDEFYDLLVTDIEEELDKIAERPEDVTNPVEKIQKEECKS 60
           MK P +FE    I  ++P++ D+F   L+ D    + + ++  +D    +++  KE   S
Sbjct: 624 MKYPDEFESVFHIDVIDPIVQDDFELNLMKDKLNLVLQQSKTDKDARREIDEDMKEAIMS 683

Query: 61  L------------------------LPSIVEPPTLEQKSLSSHLKYAYLGDNDTLPVIIA 120
           L                        LPSIV+PP LE K L +HLKY YLG+++TLPVIIA
Sbjct: 684 LHSLPIMPNMWTSLPVELPSSHVKDLPSIVQPPKLELKMLPTHLKYVYLGEDETLPVIIA 743

Query: 121 SNLSPTNEYSLLQILEKHKKSIGWTITDI 126
           ++L+  +E  LL++L +HK +IGWT+ DI
Sbjct: 744 NDLTEVHEEKLLRVLREHKTAIGWTLADI 772

[Close]

BLAST of Moc05g14660 vs. ExPASy TrEMBL
Match: A0A6P6WY27 (uncharacterized protein LOC113737344 OS=Coffea arabica OX=13443 GN=LOC113737344 PE=4 SV=1)

HSP 1 Score: 91.7 bits (226), Expect = 2.4e-15
Identity = 43/70 (61.43%), Postives = 55/70 (78.57%), Query Frame = 0


Query: 56  EECKSLLPSIVEPPTLEQKSLSSHLKYAYLGDNDTLPVIIASNLSPTNEYSLLQILEKHK 115
           E  + +LPS+V+ P LE KSL  HLKYAYLGDN+TLPVII+S LS T E  L+++L +HK
Sbjct: 343 EPHQRVLPSVVQAPVLELKSLPEHLKYAYLGDNETLPVIISSALSKTQEKKLIRVLREHK 402

Query: 116 KSIGWTITDI 126
           ++IGWTI DI
Sbjct: 403 EAIGWTIADI 412

The following BLAST results are available for this feature:

BLAST of Moc05g14660 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022151603.1	1.3e-39	90.53	LOW QUALITY PROTEIN: uncharacterized protein LOC111019515 [Momordica charantia]	[more]
XP_022158979.1	1.3e-23	90.48	uncharacterized protein LOC111025424 [Momordica charantia]	[more]
XP_030502183.1	4.3e-19	42.07	uncharacterized protein LOC115717351 [Cannabis sativa]	[more]
XP_030504461.1	1.1e-17	40.00	uncharacterized protein LOC115719521 [Cannabis sativa]	[more]
XP_021745351.1	1.8e-17	52.00	uncharacterized protein LOC110711280 [Chenopodium quinoa]	[more]

BLAST of Moc05g14660 vs. NCBI nr


Match Name	E-value	Identity	Description

BLAST of Moc05g14660 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1DCL7	8.2e-40	90.53	LOW QUALITY PROTEIN: uncharacterized protein LOC111019515 OS=Momordica charantia...	[more]
A0A6J1E110	3.7e-24	92.06	uncharacterized protein LOC111025424 OS=Momordica charantia OX=3673 GN=LOC111025...	[more]
A0A6P6UH48	6.3e-16	57.33	uncharacterized protein LOC113711211 OS=Coffea arabica OX=13443 GN=LOC113711211 ...	[more]
A0A6N2NBX1	8.2e-16	36.24	Uncharacterized protein OS=Salix viminalis OX=40686 GN=SVIM_LOCUS482486 PE=4 SV=...	[more]
A0A6P6WY27	2.4e-15	61.43	uncharacterized protein LOC113737344 OS=Coffea arabica OX=13443 GN=LOC113737344 ...	[more]

BLAST of Moc05g14660 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (OHB3-1) v2
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	PANTHER	PTHR24559:SF373	REVERSE TRANSCRIPTASE DOMAIN, RIBONUCLEASE H-LIKE DOMAIN PROTEIN-RELATED	coord: 37..125
None	No IPR available	PANTHER	PTHR24559	TRANSPOSON TY3-I GAG-POL POLYPROTEIN	coord: 37..125

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Moc05g14660.1	Moc05g14660.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0015074	DNA integration
molecular_function	GO:0003676	nucleic acid binding