Moc05g14550 (gene) Bitter gourd (OHB3-1) v2

Overview
NameMoc05g14550
Typegene
OrganismMomordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
DescriptionNAD(P)-binding Rossmann-fold superfamily protein
Locationchr5: 11000444 .. 11001367 (+)
RNA-Seq ExpressionMoc05g14550
SyntenyMoc05g14550
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCTTCACCTGAGGGAAGAATAGTCCCCGCAGCGGTTGCTCTGCCGCTGCAGGATCGAGTGGCTATCGTGACTGGCGCCTCTCGTGGCATTGGCCGAGGCGTCGCCCTCCACCTTGCTGCTCTTGGCGCCAGAGTTGTAGTAAACTACCTATCCAGTTCGGCTGCAGCCAGTCGTGTTGCTGCCGAGATCAACTCCTCCCCTGCAGCCGACATCGCGGGGAGCTACCCCCGAGCCGTTACGTGTCAGGCAGATGTGTCTAAGCCAGACGAAGTGAAGGCCCTATTCGACACTGCCGAGGAGGCATTCGGGTCCCCAGTCCATATCCTGGTCAACTCTGCCGGAATTTGGGATCCTACATATCCATATATTGCAGATACATCTTTGGAGATTTTCGACCGAATTTTCAGGTCTTCTCTATTCTAGAATACTATTTTGTACTTTCAAATGTCAAAATTTTGCTTTTTACTCTCAATTAGTGAAGCTTAAATTGAGGTGGGTGAGTATATTTTTAGTGTGAACGCAATGGGGAGTTTCCTGTGCTGTAAAGAAGCGGCAAAGAGAGTGAAAGGCGACGGTGGGGGGCGGATTATAATGATCTCGTCGTCAGCGGTGGCGGCACCGTCGCCGGGAATAGGGGCGTACACGGCGTCGAAGGCGGCGGTGGAGGCGATGACGAAGACTCTGGCAAAGGAACTGAGAGGGACGGGGATTTCGGCGAACTGCGTAGCGCCGGGAGCGACGGCGACAGACATGTTGTACGGAGGGATGGATGAGGAAGCAGTGAAGAAGGTGATAGAAAAGTGTCCGTTGGGGCGGCTTGGGTATCCGACGGACGTGGCTTATTTGGTGGGCTTTTTGGCTTCCGACGACGGTGAGTGGATCAATGGCCAGCTCTTGCTCGTCAATGGCGGCATCGTCTAG

mRNA sequence

ATGGCTTCACCTGAGGGAAGAATAGTCCCCGCAGCGGTTGCTCTGCCGCTGCAGGATCGAGTGGCTATCGTGACTGGCGCCTCTCGTGGCATTGGCCGAGGCGTCGCCCTCCACCTTGCTGCTCTTGGCGCCAGAGTTGTAGTAAACTACCTATCCAGTTCGGCTGCAGCCAGTCGTGTTGCTGCCGAGATCAACTCCTCCCCTGCAGCCGACATCGCGGGGAGCTACCCCCGAGCCGTTACGTGTCAGGCAGATGTGTCTAAGCCAGACGAAGTGAAGGCCCTATTCGACACTGCCGAGGAGGCATTCGGGTCCCCAGTCCATATCCTGGTCAACTCTGCCGGAATTTGGGATCCTACATATCCATATATTGCAGATACATCTTTGGAGATTTTCGACCGAATTTTCAGTGTGAACGCAATGGGGAGTTTCCTGTGCTGTAAAGAAGCGGCAAAGAGAGTGAAAGGCGACGGTGGGGGGCGGATTATAATGATCTCGTCGTCAGCGGTGGCGGCACCGTCGCCGGGAATAGGGGCGTACACGGCGTCGAAGGCGGCGGTGGAGGCGATGACGAAGACTCTGGCAAAGGAACTGAGAGGGACGGGGATTTCGGCGAACTGCGTAGCGCCGGGAGCGACGGCGACAGACATGTTGTACGGAGGGATGGATGAGGAAGCAGTGAAGAAGGTGATAGAAAAGTGTCCGTTGGGGCGGCTTGGGTATCCGACGGACGTGGCTTATTTGGTGGGCTTTTTGGCTTCCGACGACGGTGAGTGGATCAATGGCCAGCTCTTGCTCGTCAATGGCGGCATCGTCTAG

Coding sequence (CDS)

ATGGCTTCACCTGAGGGAAGAATAGTCCCCGCAGCGGTTGCTCTGCCGCTGCAGGATCGAGTGGCTATCGTGACTGGCGCCTCTCGTGGCATTGGCCGAGGCGTCGCCCTCCACCTTGCTGCTCTTGGCGCCAGAGTTGTAGTAAACTACCTATCCAGTTCGGCTGCAGCCAGTCGTGTTGCTGCCGAGATCAACTCCTCCCCTGCAGCCGACATCGCGGGGAGCTACCCCCGAGCCGTTACGTGTCAGGCAGATGTGTCTAAGCCAGACGAAGTGAAGGCCCTATTCGACACTGCCGAGGAGGCATTCGGGTCCCCAGTCCATATCCTGGTCAACTCTGCCGGAATTTGGGATCCTACATATCCATATATTGCAGATACATCTTTGGAGATTTTCGACCGAATTTTCAGTGTGAACGCAATGGGGAGTTTCCTGTGCTGTAAAGAAGCGGCAAAGAGAGTGAAAGGCGACGGTGGGGGGCGGATTATAATGATCTCGTCGTCAGCGGTGGCGGCACCGTCGCCGGGAATAGGGGCGTACACGGCGTCGAAGGCGGCGGTGGAGGCGATGACGAAGACTCTGGCAAAGGAACTGAGAGGGACGGGGATTTCGGCGAACTGCGTAGCGCCGGGAGCGACGGCGACAGACATGTTGTACGGAGGGATGGATGAGGAAGCAGTGAAGAAGGTGATAGAAAAGTGTCCGTTGGGGCGGCTTGGGTATCCGACGGACGTGGCTTATTTGGTGGGCTTTTTGGCTTCCGACGACGGTGAGTGGATCAATGGCCAGCTCTTGCTCGTCAATGGCGGCATCGTCTAG

Protein sequence

MASPEGRIVPAAVALPLQDRVAIVTGASRGIGRGVALHLAALGARVVVNYLSSSAAASRVAAEINSSPAADIAGSYPRAVTCQADVSKPDEVKALFDTAEEAFGSPVHILVNSAGIWDPTYPYIADTSLEIFDRIFSVNAMGSFLCCKEAAKRVKGDGGGRIIMISSSAVAAPSPGIGAYTASKAAVEAMTKTLAKELRGTGISANCVAPGATATDMLYGGMDEEAVKKVIEKCPLGRLGYPTDVAYLVGFLASDDGEWINGQLLLVNGGIV
Homology
BLAST of Moc05g14550 vs. NCBI nr
Match: XP_022155881.1 (NADPH-dependent aldehyde reductase-like protein, chloroplastic isoform X1 [Momordica charantia])

HSP 1 Score: 521.5 bits (1342), Expect = 4.2e-144
Identity = 272/272 (100.00%), Postives = 272/272 (100.00%), Query Frame = 0

Query: 1   MASPEGRIVPAAVALPLQDRVAIVTGASRGIGRGVALHLAALGARVVVNYLSSSAAASRV 60
           MASPEGRIVPAAVALPLQDRVAIVTGASRGIGRGVALHLAALGARVVVNYLSSSAAASRV
Sbjct: 23  MASPEGRIVPAAVALPLQDRVAIVTGASRGIGRGVALHLAALGARVVVNYLSSSAAASRV 82

Query: 61  AAEINSSPAADIAGSYPRAVTCQADVSKPDEVKALFDTAEEAFGSPVHILVNSAGIWDPT 120
           AAEINSSPAADIAGSYPRAVTCQADVSKPDEVKALFDTAEEAFGSPVHILVNSAGIWDPT
Sbjct: 83  AAEINSSPAADIAGSYPRAVTCQADVSKPDEVKALFDTAEEAFGSPVHILVNSAGIWDPT 142

Query: 121 YPYIADTSLEIFDRIFSVNAMGSFLCCKEAAKRVKGDGGGRIIMISSSAVAAPSPGIGAY 180
           YPYIADTSLEIFDRIFSVNAMGSFLCCKEAAKRVKGDGGGRIIMISSSAVAAPSPGIGAY
Sbjct: 143 YPYIADTSLEIFDRIFSVNAMGSFLCCKEAAKRVKGDGGGRIIMISSSAVAAPSPGIGAY 202

Query: 181 TASKAAVEAMTKTLAKELRGTGISANCVAPGATATDMLYGGMDEEAVKKVIEKCPLGRLG 240
           TASKAAVEAMTKTLAKELRGTGISANCVAPGATATDMLYGGMDEEAVKKVIEKCPLGRLG
Sbjct: 203 TASKAAVEAMTKTLAKELRGTGISANCVAPGATATDMLYGGMDEEAVKKVIEKCPLGRLG 262

Query: 241 YPTDVAYLVGFLASDDGEWINGQLLLVNGGIV 273
           YPTDVAYLVGFLASDDGEWINGQLLLVNGGIV
Sbjct: 263 YPTDVAYLVGFLASDDGEWINGQLLLVNGGIV 294

BLAST of Moc05g14550 vs. NCBI nr
Match: XP_022155882.1 (NADPH-dependent aldehyde reductase-like protein, chloroplastic isoform X2 [Momordica charantia])

HSP 1 Score: 521.5 bits (1342), Expect = 4.2e-144
Identity = 272/272 (100.00%), Postives = 272/272 (100.00%), Query Frame = 0

Query: 1   MASPEGRIVPAAVALPLQDRVAIVTGASRGIGRGVALHLAALGARVVVNYLSSSAAASRV 60
           MASPEGRIVPAAVALPLQDRVAIVTGASRGIGRGVALHLAALGARVVVNYLSSSAAASRV
Sbjct: 1   MASPEGRIVPAAVALPLQDRVAIVTGASRGIGRGVALHLAALGARVVVNYLSSSAAASRV 60

Query: 61  AAEINSSPAADIAGSYPRAVTCQADVSKPDEVKALFDTAEEAFGSPVHILVNSAGIWDPT 120
           AAEINSSPAADIAGSYPRAVTCQADVSKPDEVKALFDTAEEAFGSPVHILVNSAGIWDPT
Sbjct: 61  AAEINSSPAADIAGSYPRAVTCQADVSKPDEVKALFDTAEEAFGSPVHILVNSAGIWDPT 120

Query: 121 YPYIADTSLEIFDRIFSVNAMGSFLCCKEAAKRVKGDGGGRIIMISSSAVAAPSPGIGAY 180
           YPYIADTSLEIFDRIFSVNAMGSFLCCKEAAKRVKGDGGGRIIMISSSAVAAPSPGIGAY
Sbjct: 121 YPYIADTSLEIFDRIFSVNAMGSFLCCKEAAKRVKGDGGGRIIMISSSAVAAPSPGIGAY 180

Query: 181 TASKAAVEAMTKTLAKELRGTGISANCVAPGATATDMLYGGMDEEAVKKVIEKCPLGRLG 240
           TASKAAVEAMTKTLAKELRGTGISANCVAPGATATDMLYGGMDEEAVKKVIEKCPLGRLG
Sbjct: 181 TASKAAVEAMTKTLAKELRGTGISANCVAPGATATDMLYGGMDEEAVKKVIEKCPLGRLG 240

Query: 241 YPTDVAYLVGFLASDDGEWINGQLLLVNGGIV 273
           YPTDVAYLVGFLASDDGEWINGQLLLVNGGIV
Sbjct: 241 YPTDVAYLVGFLASDDGEWINGQLLLVNGGIV 272

BLAST of Moc05g14550 vs. NCBI nr
Match: KAG6597707.1 (NADPH-dependent aldehyde reductase-like protein, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 386.3 bits (991), Expect = 2.1e-103
Identity = 206/272 (75.74%), Postives = 231/272 (84.93%), Query Frame = 0

Query: 1   MASPEGRIVPAAVALPLQDRVAIVTGASRGIGRGVALHLAALGARVVVNYLSSSAAASRV 60
           MAS  G   PA  ALPLQDRV IVTGASRGIGR +A HLAALGAR+VVNY+SSSA A RV
Sbjct: 1   MASESG---PAMAALPLQDRVVIVTGASRGIGRAIAFHLAALGARIVVNYVSSSAKADRV 60

Query: 61  AAEINSSPAADIAGSYPRAVTCQADVSKPDEVKALFDTAEEAFGSPVHILVNSAGIWDPT 120
           AAEINSS A    GS  RA+  +ADVS P++VK+LFDTAE+ FG+ VHI+VN AGI DPT
Sbjct: 61  AAEINSSSA---TGSGLRAIAWRADVSDPEQVKSLFDTAEQTFGAQVHIMVNCAGISDPT 120

Query: 121 YPYIADTSLEIFDRIFSVNAMGSFLCCKEAAKRVKGDGGGRIIMISSSAVAAPSPGIGAY 180
           YPYIADTSLEIFD+IFSVN  GSFLCC+EAA RVK  GGGRII+ISS+AVAA + GIGAY
Sbjct: 121 YPYIADTSLEIFDQIFSVNTRGSFLCCREAANRVKRGGGGRIILISSTAVAAATAGIGAY 180

Query: 181 TASKAAVEAMTKTLAKELRGTGISANCVAPGATATDMLYGGMDEEAVKKVIEKCPLGRLG 240
           TASKAAVEAMTK  AKELRG+ I+ NC+APGATAT+MLYGGMDEEAVK+VIEKCPLGR+G
Sbjct: 181 TASKAAVEAMTKVAAKELRGSRITVNCIAPGATATEMLYGGMDEEAVKRVIEKCPLGRVG 240

Query: 241 YPTDVAYLVGFLASDDGEWINGQLLLVNGGIV 273
            P DVA  VGFLASD+GEWINGQ++LVNGGIV
Sbjct: 241 EPKDVASFVGFLASDEGEWINGQVMLVNGGIV 266

BLAST of Moc05g14550 vs. NCBI nr
Match: XP_023540764.1 (NADPH-dependent aldehyde reductase-like protein, chloroplastic [Cucurbita pepo subsp. pepo])

HSP 1 Score: 385.6 bits (989), Expect = 3.6e-103
Identity = 205/272 (75.37%), Postives = 231/272 (84.93%), Query Frame = 0

Query: 1   MASPEGRIVPAAVALPLQDRVAIVTGASRGIGRGVALHLAALGARVVVNYLSSSAAASRV 60
           MAS  G   PA  ALPLQDRV IVTGASRGIGR +A HLAALGAR+VVNY+SSSA A RV
Sbjct: 1   MASESG---PAVAALPLQDRVVIVTGASRGIGRAIAFHLAALGARIVVNYVSSSAKADRV 60

Query: 61  AAEINSSPAADIAGSYPRAVTCQADVSKPDEVKALFDTAEEAFGSPVHILVNSAGIWDPT 120
           AAEINSS A    GS  RA+  +ADVS P++VK+LFDTAE+ FG+ VHI+VN AGI DPT
Sbjct: 61  AAEINSSSA---TGSGLRAIAWRADVSDPEQVKSLFDTAEQRFGAQVHIMVNCAGISDPT 120

Query: 121 YPYIADTSLEIFDRIFSVNAMGSFLCCKEAAKRVKGDGGGRIIMISSSAVAAPSPGIGAY 180
           YPYIADTSLEIFD+IFSVN  GSFLCC+EAA RVK  GGGRII+ISS+AVAA + GIGAY
Sbjct: 121 YPYIADTSLEIFDQIFSVNTRGSFLCCREAANRVKRGGGGRIILISSTAVAAATAGIGAY 180

Query: 181 TASKAAVEAMTKTLAKELRGTGISANCVAPGATATDMLYGGMDEEAVKKVIEKCPLGRLG 240
           TASKAAVEAMTK  AKE+RG+ I+ NC+APGATAT+MLYGGMDEEAVK+VIEKCPLGR+G
Sbjct: 181 TASKAAVEAMTKVAAKEMRGSKITVNCIAPGATATEMLYGGMDEEAVKRVIEKCPLGRVG 240

Query: 241 YPTDVAYLVGFLASDDGEWINGQLLLVNGGIV 273
            P DVA  VGFLASD+GEWINGQ++LVNGGIV
Sbjct: 241 EPKDVASFVGFLASDEGEWINGQVMLVNGGIV 266

BLAST of Moc05g14550 vs. NCBI nr
Match: XP_022932775.1 (NADPH-dependent aldehyde reductase-like protein, chloroplastic [Cucurbita moschata])

HSP 1 Score: 384.4 bits (986), Expect = 8.0e-103
Identity = 206/272 (75.74%), Postives = 230/272 (84.56%), Query Frame = 0

Query: 1   MASPEGRIVPAAVALPLQDRVAIVTGASRGIGRGVALHLAALGARVVVNYLSSSAAASRV 60
           MAS  G   PA  ALPLQDRV IVTGASRGIGR +A HLAALGAR+VVNY+SSSA A RV
Sbjct: 1   MASESG---PAMAALPLQDRVVIVTGASRGIGRAIAFHLAALGARIVVNYVSSSAKADRV 60

Query: 61  AAEINSSPAADIAGSYPRAVTCQADVSKPDEVKALFDTAEEAFGSPVHILVNSAGIWDPT 120
           AAEINSS A    GS  RA+  +ADVS P++VK+LFDTAE+ FG+ VHI+VN AGI DPT
Sbjct: 61  AAEINSSSA---TGSGLRAIAWRADVSNPEQVKSLFDTAEQTFGAQVHIMVNCAGISDPT 120

Query: 121 YPYIADTSLEIFDRIFSVNAMGSFLCCKEAAKRVKGDGGGRIIMISSSAVAAPSPGIGAY 180
           YPYIADTSLEIFD+IFSVN  GSFLCC+EAA RVK  GGGRII+ISS+AVAA + GIGAY
Sbjct: 121 YPYIADTSLEIFDQIFSVNTRGSFLCCREAAIRVKRGGGGRIILISSTAVAAATAGIGAY 180

Query: 181 TASKAAVEAMTKTLAKELRGTGISANCVAPGATATDMLYGGMDEEAVKKVIEKCPLGRLG 240
           TASKAAVEAMTK  AKELRG+ I+ NC+APGATAT+MLYGGMDEEAVK+VIEKCPLGR+G
Sbjct: 181 TASKAAVEAMTKVAAKELRGSRITVNCIAPGATATEMLYGGMDEEAVKRVIEKCPLGRVG 240

Query: 241 YPTDVAYLVGFLASDDGEWINGQLLLVNGGIV 273
            P DVA  VGFLASD+GEWINGQ+ LVNGGIV
Sbjct: 241 EPKDVASFVGFLASDEGEWINGQVTLVNGGIV 266

BLAST of Moc05g14550 vs. ExPASy Swiss-Prot
Match: Q9SQR4 (NADPH-dependent aldehyde reductase-like protein, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=At3g03980 PE=3 SV=1)

HSP 1 Score: 308.1 bits (788), Expect = 9.6e-83
Identity = 161/259 (62.16%), Postives = 194/259 (74.90%), Query Frame = 0

Query: 15  LPLQDRVAIVTGASRGIGRGVALHLAALGARVVVNYLSSSAAASRVAAEINSSPA-ADIA 74
           LPL  RVAIVTG+SRGIGR +A+HLA LGAR+V+NY S +A A RVA+EIN  P   +I 
Sbjct: 12  LPLAGRVAIVTGSSRGIGRAIAIHLAELGARIVINYTSKAADAERVASEINDFPVREEIT 71

Query: 75  GSYPRAVTCQADVSKPDEVKALFDTAEEAFGSPVHILVNSAGIWDPTYPYIADTSLEIFD 134
           G  PRA+  QA+VS+P +VK++FD AE AF +PVHILVNSAGI DP YP IADTS+E FD
Sbjct: 72  GKGPRAIVVQANVSEPSQVKSMFDAAESAFEAPVHILVNSAGILDPKYPTIADTSVEDFD 131

Query: 135 RIFSVNAMGSFLCCKEAAKRVKGDGGGRIIMISSSAVAAPSPGIGAYTASKAAVEAMTKT 194
             FSVN  G+FLC KEAA R+K  GGGRII+++SS   +  PG GAY ASKAAVE M K 
Sbjct: 132 HTFSVNTKGAFLCSKEAANRLKQGGGGRIILLTSSQTRSLKPGFGAYAASKAAVETMVKI 191

Query: 195 LAKELRGTGISANCVAPGATATDMLYGGMDEEAVKKVIEKCPLGRLGYPTDVAYLVGFLA 254
           LAKEL+GTGI+ANCVAPG  AT+M + G   E V+K+  + P GR+G   DV  LVGFLA
Sbjct: 192 LAKELKGTGITANCVAPGPIATEMFFDGKTPELVEKIAAESPFGRVGEAKDVVPLVGFLA 251

Query: 255 SDDGEWINGQLLLVNGGIV 273
            D GEW+NGQ++ VNGG V
Sbjct: 252 GDGGEWVNGQIIPVNGGYV 270

BLAST of Moc05g14550 vs. ExPASy Swiss-Prot
Match: Q9SQR2 (NADPH-dependent aldehyde reductase 2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=ChlADR2 PE=1 SV=1)

HSP 1 Score: 282.0 bits (720), Expect = 7.4e-75
Identity = 150/258 (58.14%), Postives = 189/258 (73.26%), Query Frame = 0

Query: 15  LPLQDRVAIVTGASRGIGRGVALHLAALGARVVVNYLSSSAAASRVAAEI--NSSPAADI 74
           L L  RVAIVTG+SRGIGR +A+HLA LGARVVVNY +S   A +VA  I  N S  A++
Sbjct: 12  LCLAGRVAIVTGSSRGIGRAIAIHLAELGARVVVNYSTSPVEAEKVATAITTNCSKDAEV 71

Query: 75  AGSYPRAVTCQADVSKPDEVKALFDTAEEAFGSPVHILVNSAGIWDPTYPYIADTSLEIF 134
           AG  PR +  +AD+S+P +VK+LFD AE  F SPVHILVNSA I DP +  I+D S+E+F
Sbjct: 72  AGKSPRVIVVKADISEPSQVKSLFDEAERVFESPVHILVNSAAIADPNHSTISDMSVELF 131

Query: 135 DRIFSVNAMGSFLCCKEAAKRVKGDGGGRIIMISSSAVAAPSPGIGAYTASKAAVEAMTK 194
           DRI SVN  G+F+C +EAA R+K  GGGRII++S+S V   +   G+YTASKAAVEAM K
Sbjct: 132 DRIISVNTRGAFICAREAANRLKRGGGGRIILLSTSLVQTLNTNYGSYTASKAAVEAMAK 191

Query: 195 TLAKELRGTGISANCVAPGATATDMLYGGMDEEAVKKVIEKCPLGRLGYPTDVAYLVGFL 254
            LAKEL+GT I+ NCV+PG  AT+M Y G+  E V+KV  +   GR+G   D+A +VGFL
Sbjct: 192 ILAKELKGTEITVNCVSPGPVATEMFYTGLSNEIVEKVKSQNLFGRIGETKDIAPVVGFL 251

Query: 255 ASDDGEWINGQLLLVNGG 271
           ASD GEWINGQ+++ NGG
Sbjct: 252 ASDAGEWINGQVIMANGG 269

BLAST of Moc05g14550 vs. ExPASy Swiss-Prot
Match: Q08632 (Short-chain type dehydrogenase/reductase OS=Picea abies OX=3329 PE=2 SV=1)

HSP 1 Score: 275.0 bits (702), Expect = 9.0e-73
Identity = 154/258 (59.69%), Postives = 189/258 (73.26%), Query Frame = 0

Query: 15  LPLQDRVAIVTGASRGIGRGVALHLAALGARVVVNYLSSSAAASRVAAEINSSPAADIAG 74
           LPL  RVAIVTGASRGIGR +AL++A  GA+VV++Y S+  AA  VA+ IN+   +  +G
Sbjct: 17  LPLGGRVAIVTGASRGIGREIALNMAEKGAKVVIHYSSNQHAAEEVASIINNKSPS--SG 76

Query: 75  SYPRAVTCQADVSKPDEVKALFDTAEEAFGSPVHILVNSAGIWDPTYPYIADTSLEIFDR 134
              RA+ C+ADV++P +V  LFDTAE AFG P+HI+VN+AG+ D  YP +A TS E +DR
Sbjct: 77  DGVRAIVCKADVAEPSQVAQLFDTAEHAFG-PLHIVVNNAGVTDSKYPTLAQTSDEEWDR 136

Query: 135 IFSVNAMGSFLCCKEAAKRVKGDGGGRIIMISSSAVAAPSPGIGAYTASKAAVEAMTKTL 194
           IF VN  G+FLC +EAAKRV   GGGRII ISSS VA P P  GAYTASKAAVE MT+ L
Sbjct: 137 IFQVNCKGAFLCSREAAKRVVRGGGGRIINISSSLVAMPIPRYGAYTASKAAVEMMTRIL 196

Query: 195 AKELRGTGISANCVAPGATATDMLYGGMDEEAVKKVIEKCPLGRLGYPTDVAYLVGFLAS 254
           A+ELRGT I+ANCVAPG  ATDM + G  E AV+  ++  P  RLG   DVA LV FLAS
Sbjct: 197 AQELRGTQITANCVAPGPVATDMFFAGKSEAAVEAGVKSNPFERLGKVEDVAPLVAFLAS 256

Query: 255 DDGEWINGQLLLVNGGIV 273
           D+GEW+N Q++ VNGG V
Sbjct: 257 DEGEWVNAQVVRVNGGQV 271

BLAST of Moc05g14550 vs. ExPASy Swiss-Prot
Match: W3XC32 (Trihydroxynaphthalene reductase PfmaI OS=Pestalotiopsis fici (strain W106-1 / CGMCC3.15140) OX=1229662 GN=Pfmaj PE=2 SV=1)

HSP 1 Score: 156.0 bits (393), Expect = 6.1e-37
Identity = 103/265 (38.87%), Postives = 157/265 (59.25%), Query Frame = 0

Query: 17  LQDRVAIVTGASRGIGRGVALHLAALGARVVVNYLSSSAAASRVAAEINSSPAADIAGSY 76
           L+ +VA+VTGA RGIGR +A+ L   GA+V+VNY +SS +A  V   I  S      GS 
Sbjct: 27  LEGKVALVTGAGRGIGREMAMELGRRGAKVIVNYANSSESAQEVVNAIKKS------GS- 86

Query: 77  PRAVTCQADVSKPDEVKALFDTAEEAFGSPVHILVNSAGIWDPTYPYIADTSLEIFDRIF 136
             AV  +A+VS  D++ +LFD A + +G  +HI+ +++G+   ++ ++ D + E FDR+F
Sbjct: 87  -DAVAIKANVSDVDQIVSLFDQAVKVWGK-LHIVCSNSGV--VSFGHVKDVTPEEFDRVF 146

Query: 137 SVNAMGSFLCCKEAAKRVKGDGGGRIIMISS-SAVAAPSPGIGAYTASKAAVEAMTKTLA 196
           ++N  G F   +EA K +  + GGR+I++ S +  A   P    Y+ SK A+E   + +A
Sbjct: 147 TINTRGQFFVAREAYKHL--EVGGRLILMGSITGQAKGVPKHAVYSGSKGAIETFVRCMA 206

Query: 197 KELRGTGISANCVAPGATATDMLY--------GG--MDEEAVKKVIEK-CPLGRLGYPTD 256
            +     I+ N VAPG   TDM +        GG  +D+EAV +      PLGR+G P D
Sbjct: 207 VDFGDKKITVNAVAPGGIKTDMYHAVCREYIPGGTELDDEAVDEYASTWSPLGRVGLPID 266

Query: 257 VAYLVGFLASDDGEWINGQLLLVNG 270
           +A  V FLAS DGEW+NG++L ++G
Sbjct: 267 IARCVCFLASQDGEWVNGKVLGIDG 278

BLAST of Moc05g14550 vs. ExPASy Swiss-Prot
Match: A0A411PQN6 (Short chain dehydrogenase AgnL6 OS=Paecilomyces divaricatus OX=644132 GN=AgnL6 PE=3 SV=1)

HSP 1 Score: 154.5 bits (389), Expect = 1.8e-36
Identity = 106/273 (38.83%), Postives = 158/273 (57.88%), Query Frame = 0

Query: 10  PAAVALPLQDRVAIVTGASRGIGRGVALHLAALGARVVVNYLSSSAAASRVAAEINSSPA 69
           P  +   L  +VA+VTGA RGIG  +A+ LA  GA+V+VNY  SS+ A+ V  EI S   
Sbjct: 7   PPHLPYRLDGKVALVTGAGRGIGAAMAVELARCGAKVIVNYARSSSFANAVVDEIKS--- 66

Query: 70  ADIAGSYPRAVTCQADVSKPDEVKALFDTAEEAFGSPVHILVNSAGIWDPTYPYIADTSL 129
               GS   AV  QADVS+  +   LF+ A + FG  + I+ ++AG+   ++ ++++ + 
Sbjct: 67  ---LGS--DAVAIQADVSQVSQTVRLFEEAVQVFGG-LDIVCSNAGV--VSFGHLSEVTE 126

Query: 130 EIFDRIFSVNAMGSFLCCKEAAKRVKGDGGGRIIMISS-SAVAAPSPGIGAYTASKAAVE 189
           E FDR+FSVN  G F   +EA + +  +  GRII++SS +A +   P    Y+ASK A+ 
Sbjct: 127 EEFDRVFSVNTRGQFFVAREAYRHL--NSPGRIILMSSNTADSFTVPKHSLYSASKGAIN 186

Query: 190 AMTKTLAKELRGTGISANCVAPGATATDMLY----------GGMDEEAVKKVIEK-CPLG 249
              + LAK+     I+ N VAPG T TDM +            M +E + +++    PL 
Sbjct: 187 TFVRCLAKDCGDKKITVNAVAPGGTVTDMFHETAKDYLPNADKMSKEQILQIVANVSPLT 246

Query: 250 RLGYPTDVAYLVGFLASDDGEWINGQLLLVNGG 271
           R GYP D+A +V FLAS + EW+NG++L V+GG
Sbjct: 247 RCGYPIDIAKVVCFLASSESEWVNGKVLGVDGG 266

BLAST of Moc05g14550 vs. ExPASy TrEMBL
Match: A0A6J1DQK1 (NADPH-dependent aldehyde reductase-like protein, chloroplastic isoform X2 OS=Momordica charantia OX=3673 GN=LOC111022892 PE=4 SV=1)

HSP 1 Score: 521.5 bits (1342), Expect = 2.0e-144
Identity = 272/272 (100.00%), Postives = 272/272 (100.00%), Query Frame = 0

Query: 1   MASPEGRIVPAAVALPLQDRVAIVTGASRGIGRGVALHLAALGARVVVNYLSSSAAASRV 60
           MASPEGRIVPAAVALPLQDRVAIVTGASRGIGRGVALHLAALGARVVVNYLSSSAAASRV
Sbjct: 1   MASPEGRIVPAAVALPLQDRVAIVTGASRGIGRGVALHLAALGARVVVNYLSSSAAASRV 60

Query: 61  AAEINSSPAADIAGSYPRAVTCQADVSKPDEVKALFDTAEEAFGSPVHILVNSAGIWDPT 120
           AAEINSSPAADIAGSYPRAVTCQADVSKPDEVKALFDTAEEAFGSPVHILVNSAGIWDPT
Sbjct: 61  AAEINSSPAADIAGSYPRAVTCQADVSKPDEVKALFDTAEEAFGSPVHILVNSAGIWDPT 120

Query: 121 YPYIADTSLEIFDRIFSVNAMGSFLCCKEAAKRVKGDGGGRIIMISSSAVAAPSPGIGAY 180
           YPYIADTSLEIFDRIFSVNAMGSFLCCKEAAKRVKGDGGGRIIMISSSAVAAPSPGIGAY
Sbjct: 121 YPYIADTSLEIFDRIFSVNAMGSFLCCKEAAKRVKGDGGGRIIMISSSAVAAPSPGIGAY 180

Query: 181 TASKAAVEAMTKTLAKELRGTGISANCVAPGATATDMLYGGMDEEAVKKVIEKCPLGRLG 240
           TASKAAVEAMTKTLAKELRGTGISANCVAPGATATDMLYGGMDEEAVKKVIEKCPLGRLG
Sbjct: 181 TASKAAVEAMTKTLAKELRGTGISANCVAPGATATDMLYGGMDEEAVKKVIEKCPLGRLG 240

Query: 241 YPTDVAYLVGFLASDDGEWINGQLLLVNGGIV 273
           YPTDVAYLVGFLASDDGEWINGQLLLVNGGIV
Sbjct: 241 YPTDVAYLVGFLASDDGEWINGQLLLVNGGIV 272

BLAST of Moc05g14550 vs. ExPASy TrEMBL
Match: A0A6J1DT22 (NADPH-dependent aldehyde reductase-like protein, chloroplastic isoform X1 OS=Momordica charantia OX=3673 GN=LOC111022892 PE=4 SV=1)

HSP 1 Score: 521.5 bits (1342), Expect = 2.0e-144
Identity = 272/272 (100.00%), Postives = 272/272 (100.00%), Query Frame = 0

Query: 1   MASPEGRIVPAAVALPLQDRVAIVTGASRGIGRGVALHLAALGARVVVNYLSSSAAASRV 60
           MASPEGRIVPAAVALPLQDRVAIVTGASRGIGRGVALHLAALGARVVVNYLSSSAAASRV
Sbjct: 23  MASPEGRIVPAAVALPLQDRVAIVTGASRGIGRGVALHLAALGARVVVNYLSSSAAASRV 82

Query: 61  AAEINSSPAADIAGSYPRAVTCQADVSKPDEVKALFDTAEEAFGSPVHILVNSAGIWDPT 120
           AAEINSSPAADIAGSYPRAVTCQADVSKPDEVKALFDTAEEAFGSPVHILVNSAGIWDPT
Sbjct: 83  AAEINSSPAADIAGSYPRAVTCQADVSKPDEVKALFDTAEEAFGSPVHILVNSAGIWDPT 142

Query: 121 YPYIADTSLEIFDRIFSVNAMGSFLCCKEAAKRVKGDGGGRIIMISSSAVAAPSPGIGAY 180
           YPYIADTSLEIFDRIFSVNAMGSFLCCKEAAKRVKGDGGGRIIMISSSAVAAPSPGIGAY
Sbjct: 143 YPYIADTSLEIFDRIFSVNAMGSFLCCKEAAKRVKGDGGGRIIMISSSAVAAPSPGIGAY 202

Query: 181 TASKAAVEAMTKTLAKELRGTGISANCVAPGATATDMLYGGMDEEAVKKVIEKCPLGRLG 240
           TASKAAVEAMTKTLAKELRGTGISANCVAPGATATDMLYGGMDEEAVKKVIEKCPLGRLG
Sbjct: 203 TASKAAVEAMTKTLAKELRGTGISANCVAPGATATDMLYGGMDEEAVKKVIEKCPLGRLG 262

Query: 241 YPTDVAYLVGFLASDDGEWINGQLLLVNGGIV 273
           YPTDVAYLVGFLASDDGEWINGQLLLVNGGIV
Sbjct: 263 YPTDVAYLVGFLASDDGEWINGQLLLVNGGIV 294

BLAST of Moc05g14550 vs. ExPASy TrEMBL
Match: A0A6J1F321 (NADPH-dependent aldehyde reductase-like protein, chloroplastic OS=Cucurbita moschata OX=3662 GN=LOC111439224 PE=4 SV=1)

HSP 1 Score: 384.4 bits (986), Expect = 3.9e-103
Identity = 206/272 (75.74%), Postives = 230/272 (84.56%), Query Frame = 0

Query: 1   MASPEGRIVPAAVALPLQDRVAIVTGASRGIGRGVALHLAALGARVVVNYLSSSAAASRV 60
           MAS  G   PA  ALPLQDRV IVTGASRGIGR +A HLAALGAR+VVNY+SSSA A RV
Sbjct: 1   MASESG---PAMAALPLQDRVVIVTGASRGIGRAIAFHLAALGARIVVNYVSSSAKADRV 60

Query: 61  AAEINSSPAADIAGSYPRAVTCQADVSKPDEVKALFDTAEEAFGSPVHILVNSAGIWDPT 120
           AAEINSS A    GS  RA+  +ADVS P++VK+LFDTAE+ FG+ VHI+VN AGI DPT
Sbjct: 61  AAEINSSSA---TGSGLRAIAWRADVSNPEQVKSLFDTAEQTFGAQVHIMVNCAGISDPT 120

Query: 121 YPYIADTSLEIFDRIFSVNAMGSFLCCKEAAKRVKGDGGGRIIMISSSAVAAPSPGIGAY 180
           YPYIADTSLEIFD+IFSVN  GSFLCC+EAA RVK  GGGRII+ISS+AVAA + GIGAY
Sbjct: 121 YPYIADTSLEIFDQIFSVNTRGSFLCCREAAIRVKRGGGGRIILISSTAVAAATAGIGAY 180

Query: 181 TASKAAVEAMTKTLAKELRGTGISANCVAPGATATDMLYGGMDEEAVKKVIEKCPLGRLG 240
           TASKAAVEAMTK  AKELRG+ I+ NC+APGATAT+MLYGGMDEEAVK+VIEKCPLGR+G
Sbjct: 181 TASKAAVEAMTKVAAKELRGSRITVNCIAPGATATEMLYGGMDEEAVKRVIEKCPLGRVG 240

Query: 241 YPTDVAYLVGFLASDDGEWINGQLLLVNGGIV 273
            P DVA  VGFLASD+GEWINGQ+ LVNGGIV
Sbjct: 241 EPKDVASFVGFLASDEGEWINGQVTLVNGGIV 266

BLAST of Moc05g14550 vs. ExPASy TrEMBL
Match: A0A0A0LI83 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G843800 PE=4 SV=1)

HSP 1 Score: 381.7 bits (979), Expect = 2.5e-102
Identity = 204/272 (75.00%), Postives = 227/272 (83.46%), Query Frame = 0

Query: 1   MASPEGRIVPAAVALPLQDRVAIVTGASRGIGRGVALHLAALGARVVVNYLSSSAAASRV 60
           MAS  G   PA  ALPLQDRVAIVTGASRGIGRG+ALHLA LGARVVVNY+SSSA A +V
Sbjct: 1   MASESG---PAVPALPLQDRVAIVTGASRGIGRGIALHLAGLGARVVVNYVSSSAEADQV 60

Query: 61  AAEINSSPAADIAGSYPRAVTCQADVSKPDEVKALFDTAEEAFGSPVHILVNSAGIWDPT 120
            A+INSS A   AGS  RA+  +ADVS P++VK+LFD AE+AFGS VHILVNSAGI DPT
Sbjct: 61  VADINSSSA---AGSSQRAIAWRADVSDPEQVKSLFDAAEQAFGSQVHILVNSAGISDPT 120

Query: 121 YPYIADTSLEIFDRIFSVNAMGSFLCCKEAAKRVKGDGGGRIIMISSSAVAAPSPGIGAY 180
           YPYIA+T LEIFD +FSVN  G FLCCKEAA RVK  GGGRII+ISS+AVAA + G+GAY
Sbjct: 121 YPYIANTPLEIFDHLFSVNTRGCFLCCKEAANRVKRGGGGRIILISSTAVAATTAGLGAY 180

Query: 181 TASKAAVEAMTKTLAKELRGTGISANCVAPGATATDMLYGGMDEEAVKKVIEKCPLGRLG 240
           TASKAAVEAM K  AKEL GTGIS NC+APGATAT+M Y G+DEE VKKVI+KCP+GRLG
Sbjct: 181 TASKAAVEAMAKVTAKELSGTGISVNCIAPGATATEMFYKGIDEEGVKKVIDKCPMGRLG 240

Query: 241 YPTDVAYLVGFLASDDGEWINGQLLLVNGGIV 273
            P DVA  VGFLASDDGEWINGQ++LVNGGIV
Sbjct: 241 VPKDVASFVGFLASDDGEWINGQVILVNGGIV 266

BLAST of Moc05g14550 vs. ExPASy TrEMBL
Match: A0A5A7U7W3 (Photosynthetic NDH subunit of subcomplex B 2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold734G00100 PE=4 SV=1)

HSP 1 Score: 377.5 bits (968), Expect = 4.8e-101
Identity = 201/272 (73.90%), Postives = 225/272 (82.72%), Query Frame = 0

Query: 1   MASPEGRIVPAAVALPLQDRVAIVTGASRGIGRGVALHLAALGARVVVNYLSSSAAASRV 60
           MAS  G   PA  ALPLQDRVAIVTGASRGIGRG+ALHLA LGARVVVNY++SSA A +V
Sbjct: 365 MASESG---PAVPALPLQDRVAIVTGASRGIGRGIALHLAGLGARVVVNYVASSAEADQV 424

Query: 61  AAEINSSPAADIAGSYPRAVTCQADVSKPDEVKALFDTAEEAFGSPVHILVNSAGIWDPT 120
            A INS+ A   AGS  RA+  +ADVS P++VK+LFD AE+AFGS VHILVNSAGI DPT
Sbjct: 425 VASINSTSA---AGSSQRAIAWRADVSDPEQVKSLFDAAEQAFGSQVHILVNSAGISDPT 484

Query: 121 YPYIADTSLEIFDRIFSVNAMGSFLCCKEAAKRVKGDGGGRIIMISSSAVAAPSPGIGAY 180
           YPYIADT LEIFD +FSVN  G FLCCKEAA RVK  GGGRII+ISS+AV A + G+GAY
Sbjct: 485 YPYIADTPLEIFDHLFSVNTRGCFLCCKEAANRVKRGGGGRIILISSTAVVATTAGLGAY 544

Query: 181 TASKAAVEAMTKTLAKELRGTGISANCVAPGATATDMLYGGMDEEAVKKVIEKCPLGRLG 240
           TASKAAVEAM K  AKEL GTGIS NC+APGATAT+M Y G++EE VKKVIEKCP+GR+G
Sbjct: 545 TASKAAVEAMAKVAAKELSGTGISVNCIAPGATATEMFYKGINEEGVKKVIEKCPMGRIG 604

Query: 241 YPTDVAYLVGFLASDDGEWINGQLLLVNGGIV 273
            P DVA  VGFLASDDGEWINGQ++LVNGGIV
Sbjct: 605 VPKDVASFVGFLASDDGEWINGQVILVNGGIV 630

BLAST of Moc05g14550 vs. TAIR 10
Match: AT3G03980.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 308.1 bits (788), Expect = 6.8e-84
Identity = 161/259 (62.16%), Postives = 194/259 (74.90%), Query Frame = 0

Query: 15  LPLQDRVAIVTGASRGIGRGVALHLAALGARVVVNYLSSSAAASRVAAEINSSPA-ADIA 74
           LPL  RVAIVTG+SRGIGR +A+HLA LGAR+V+NY S +A A RVA+EIN  P   +I 
Sbjct: 12  LPLAGRVAIVTGSSRGIGRAIAIHLAELGARIVINYTSKAADAERVASEINDFPVREEIT 71

Query: 75  GSYPRAVTCQADVSKPDEVKALFDTAEEAFGSPVHILVNSAGIWDPTYPYIADTSLEIFD 134
           G  PRA+  QA+VS+P +VK++FD AE AF +PVHILVNSAGI DP YP IADTS+E FD
Sbjct: 72  GKGPRAIVVQANVSEPSQVKSMFDAAESAFEAPVHILVNSAGILDPKYPTIADTSVEDFD 131

Query: 135 RIFSVNAMGSFLCCKEAAKRVKGDGGGRIIMISSSAVAAPSPGIGAYTASKAAVEAMTKT 194
             FSVN  G+FLC KEAA R+K  GGGRII+++SS   +  PG GAY ASKAAVE M K 
Sbjct: 132 HTFSVNTKGAFLCSKEAANRLKQGGGGRIILLTSSQTRSLKPGFGAYAASKAAVETMVKI 191

Query: 195 LAKELRGTGISANCVAPGATATDMLYGGMDEEAVKKVIEKCPLGRLGYPTDVAYLVGFLA 254
           LAKEL+GTGI+ANCVAPG  AT+M + G   E V+K+  + P GR+G   DV  LVGFLA
Sbjct: 192 LAKELKGTGITANCVAPGPIATEMFFDGKTPELVEKIAAESPFGRVGEAKDVVPLVGFLA 251

Query: 255 SDDGEWINGQLLLVNGGIV 273
            D GEW+NGQ++ VNGG V
Sbjct: 252 GDGGEWVNGQIIPVNGGYV 270

BLAST of Moc05g14550 vs. TAIR 10
Match: AT4G13180.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 286.6 bits (732), Expect = 2.1e-77
Identity = 145/259 (55.98%), Postives = 185/259 (71.43%), Query Frame = 0

Query: 14  ALPLQDRVAIVTGASRGIGRGVALHLAALGARVVVNYLSSSAAASRVAAEINSSPAADIA 73
           +LPL  RVAIVTGA+RG+GR +A+HL +LGARV +NY+SSS+ A  + +E+N S      
Sbjct: 10  SLPLAGRVAIVTGATRGMGREIAIHLHSLGARVTINYVSSSSKAELLVSELNDS------ 69

Query: 74  GSYPRAVTCQADVSKPDEVKALFDTAEEAFGSPVHILVNSAGIWDPTYPYIADTSLEIFD 133
                A+  +ADVS PD++  LFD  E+ FGS VHI+VN AG+ DP YP +++T+LE FD
Sbjct: 70  SQLKSAIAVKADVSDPDQINNLFDQTEQEFGSKVHIVVNCAGVLDPKYPSLSETTLEDFD 129

Query: 134 RIFSVNAMGSFLCCKEAAKRVKGDGGGRIIMISSSAVAAPSPGIGAYTASKAAVEAMTKT 193
             F++N  GSFLCCKEAAKRV   GGGRIIM+S+S V   +PG G Y ASKAAVE M K 
Sbjct: 130 NTFTINTRGSFLCCKEAAKRVMRGGGGRIIMMSTSMVGGLAPGYGVYAASKAAVETMVKV 189

Query: 194 LAKELRGTGISANCVAPGATATDMLYGGMDEEAVKKVIEKCPLGRLGYPTDVAYLVGFLA 253
           LAKEL+G+ I+ANCVAPG  AT+M Y G  +E VK +   CP+GR+G   D+  +VGFLA
Sbjct: 190 LAKELKGSRITANCVAPGPVATEMFYAGKSDETVKMLAGACPMGRIGESKDITEIVGFLA 249

Query: 254 SDDGEWINGQLLLVNGGIV 273
            D GEWINGQ++  NGG V
Sbjct: 250 GDGGEWINGQVIRANGGFV 262

BLAST of Moc05g14550 vs. TAIR 10
Match: AT5G18210.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 285.0 bits (728), Expect = 6.2e-77
Identity = 153/243 (62.96%), Postives = 185/243 (76.13%), Query Frame = 0

Query: 17  LQDRVAIVTGASRGIGRGVALHLAALGARVVVNYLSSSAAASRVAAEINSSPAADIAGSY 76
           L  RVAIVTG+SRGIGR +A+HLA LGA++V+NY + S  A +VAAEINSS     AG+ 
Sbjct: 8   LAGRVAIVTGSSRGIGRAIAIHLAELGAKIVINYTTRSTEADQVAAEINSS-----AGTV 67

Query: 77  PR--AVTCQADVSKPDEVKALFDTAEEAFGSPVHILVNSAGIWDPTYPYIADTSLEIFDR 136
           P+  AV   AD+S+P ++K+LFD AE+AF SPVHILVNSAGI +P YP IA+T +E FDR
Sbjct: 68  PQPIAVVFLADISEPSQIKSLFDAAEKAFNSPVHILVNSAGILNPNYPTIANTPIEEFDR 127

Query: 137 IFSVNAMGSFLCCKEAAKRVKGDGGGRIIMISSSAVAAPSPGIGAYTASKAAVEAMTKTL 196
           IF VN  GSFLCCKEAAKR+K  GGGRII+++SS   A  PG GAYTASKAAVEAM K L
Sbjct: 128 IFKVNTRGSFLCCKEAAKRLKRGGGGRIILLTSSLTEALIPGQGAYTASKAAVEAMVKIL 187

Query: 197 AKELRGTGISANCVAPGATATDMLYGGMDEEAVKKVIEKCPLGRLGYPTDVAYLVGFLAS 256
           AKEL+G GI+ANCV+PG  AT+M + G  EE V  +IE+ P GRLG   D+A +VGFLAS
Sbjct: 188 AKELKGLGITANCVSPGPVATEMFFDGKSEETVMNIIERSPFGRLGETKDIASVVGFLAS 245

Query: 257 DDG 258
           D G
Sbjct: 248 DGG 245

BLAST of Moc05g14550 vs. TAIR 10
Match: AT3G04000.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 282.0 bits (720), Expect = 5.2e-76
Identity = 150/258 (58.14%), Postives = 189/258 (73.26%), Query Frame = 0

Query: 15  LPLQDRVAIVTGASRGIGRGVALHLAALGARVVVNYLSSSAAASRVAAEI--NSSPAADI 74
           L L  RVAIVTG+SRGIGR +A+HLA LGARVVVNY +S   A +VA  I  N S  A++
Sbjct: 12  LCLAGRVAIVTGSSRGIGRAIAIHLAELGARVVVNYSTSPVEAEKVATAITTNCSKDAEV 71

Query: 75  AGSYPRAVTCQADVSKPDEVKALFDTAEEAFGSPVHILVNSAGIWDPTYPYIADTSLEIF 134
           AG  PR +  +AD+S+P +VK+LFD AE  F SPVHILVNSA I DP +  I+D S+E+F
Sbjct: 72  AGKSPRVIVVKADISEPSQVKSLFDEAERVFESPVHILVNSAAIADPNHSTISDMSVELF 131

Query: 135 DRIFSVNAMGSFLCCKEAAKRVKGDGGGRIIMISSSAVAAPSPGIGAYTASKAAVEAMTK 194
           DRI SVN  G+F+C +EAA R+K  GGGRII++S+S V   +   G+YTASKAAVEAM K
Sbjct: 132 DRIISVNTRGAFICAREAANRLKRGGGGRIILLSTSLVQTLNTNYGSYTASKAAVEAMAK 191

Query: 195 TLAKELRGTGISANCVAPGATATDMLYGGMDEEAVKKVIEKCPLGRLGYPTDVAYLVGFL 254
            LAKEL+GT I+ NCV+PG  AT+M Y G+  E V+KV  +   GR+G   D+A +VGFL
Sbjct: 192 ILAKELKGTEITVNCVSPGPVATEMFYTGLSNEIVEKVKSQNLFGRIGETKDIAPVVGFL 251

Query: 255 ASDDGEWINGQLLLVNGG 271
           ASD GEWINGQ+++ NGG
Sbjct: 252 ASDAGEWINGQVIMANGG 269

BLAST of Moc05g14550 vs. TAIR 10
Match: AT1G24360.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 130.6 bits (327), Expect = 2.0e-30
Identity = 96/263 (36.50%), Postives = 140/263 (53.23%), Query Frame = 0

Query: 10  PAAVALPLQDRVAIVTGASRGIGRGVALHLAALGARVVVNYLSSSAAASRVAAEINSSPA 69
           P  V   ++  V ++TGASRGIG+ +AL L   G +V+VNY  S+  A  VA +I     
Sbjct: 67  PGEVVQKVESPVVVITGASRGIGKAIALALGKAGCKVLVNYARSAKEAEEVAKQIE---- 126

Query: 70  ADIAGSYPRAVTCQADVSKPDEVKALFDTAEEAFGSPVHILVNSAGIWDPTYPYIADTSL 129
            +  G   +A+T   DVSK  +V A+  TA + +G+ + ++VN+AGI   T   +     
Sbjct: 127 -EYGG---QAITFGGDVSKATDVDAMMKTALDKWGT-IDVVVNNAGITRDT--LLIRMKQ 186

Query: 130 EIFDRIFSVNAMGSFLCCKEAAKRVKGDGGGRIIMISSSAVAAPSPGIGAYTASKAAVEA 189
             +D + ++N  G FLC + A K +     GRII ISS      + G   Y A+K  V +
Sbjct: 187 SQWDEVIALNLTGVFLCTQAAVKIMMKKKRGRIINISSVVGLIGNIGQANYAAAKGGVIS 246

Query: 190 MTKTLAKELRGTGISANCVAPGATATDMLYGGMDEEAVKKVIEKCPLGRLGYPTDVAYLV 249
            +KT A+E     I+ N V PG  A+DM    + E+  KK++   PLGR G   +VA LV
Sbjct: 247 FSKTAAREGASRNINVNVVCPGFIASDMT-AELGEDMEKKILGTIPLGRYGKAEEVAGLV 306

Query: 250 GFLA-SDDGEWINGQLLLVNGGI 272
            FLA S    +I GQ   ++GGI
Sbjct: 307 EFLALSPAASYITGQAFTIDGGI 317

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022155881.14.2e-144100.00NADPH-dependent aldehyde reductase-like protein, chloroplastic isoform X1 [Momor... [more]
XP_022155882.14.2e-144100.00NADPH-dependent aldehyde reductase-like protein, chloroplastic isoform X2 [Momor... [more]
KAG6597707.12.1e-10375.74NADPH-dependent aldehyde reductase-like protein, chloroplastic, partial [Cucurbi... [more]
XP_023540764.13.6e-10375.37NADPH-dependent aldehyde reductase-like protein, chloroplastic [Cucurbita pepo s... [more]
XP_022932775.18.0e-10375.74NADPH-dependent aldehyde reductase-like protein, chloroplastic [Cucurbita moscha... [more]
Match NameE-valueIdentityDescription
Q9SQR49.6e-8362.16NADPH-dependent aldehyde reductase-like protein, chloroplastic OS=Arabidopsis th... [more]
Q9SQR27.4e-7558.14NADPH-dependent aldehyde reductase 2, chloroplastic OS=Arabidopsis thaliana OX=3... [more]
Q086329.0e-7359.69Short-chain type dehydrogenase/reductase OS=Picea abies OX=3329 PE=2 SV=1[more]
W3XC326.1e-3738.87Trihydroxynaphthalene reductase PfmaI OS=Pestalotiopsis fici (strain W106-1 / CG... [more]
A0A411PQN61.8e-3638.83Short chain dehydrogenase AgnL6 OS=Paecilomyces divaricatus OX=644132 GN=AgnL6 P... [more]
Match NameE-valueIdentityDescription
A0A6J1DQK12.0e-144100.00NADPH-dependent aldehyde reductase-like protein, chloroplastic isoform X2 OS=Mom... [more]
A0A6J1DT222.0e-144100.00NADPH-dependent aldehyde reductase-like protein, chloroplastic isoform X1 OS=Mom... [more]
A0A6J1F3213.9e-10375.74NADPH-dependent aldehyde reductase-like protein, chloroplastic OS=Cucurbita mosc... [more]
A0A0A0LI832.5e-10275.00Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G843800 PE=4 SV=1[more]
A0A5A7U7W34.8e-10173.90Photosynthetic NDH subunit of subcomplex B 2 OS=Cucumis melo var. makuwa OX=1194... [more]
Match NameE-valueIdentityDescription
AT3G03980.16.8e-8462.16NAD(P)-binding Rossmann-fold superfamily protein [more]
AT4G13180.12.1e-7755.98NAD(P)-binding Rossmann-fold superfamily protein [more]
AT5G18210.16.2e-7762.96NAD(P)-binding Rossmann-fold superfamily protein [more]
AT3G04000.15.2e-7658.14NAD(P)-binding Rossmann-fold superfamily protein [more]
AT1G24360.12.0e-3036.50NAD(P)-binding Rossmann-fold superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (OHB3-1) v2
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002347Short-chain dehydrogenase/reductase SDRPRINTSPR00081GDHRDHcoord: 235..255
score: 43.46
coord: 180..199
score: 44.01
coord: 201..218
score: 41.12
coord: 105..116
score: 42.85
coord: 154..170
score: 32.16
coord: 21..38
score: 49.13
IPR002347Short-chain dehydrogenase/reductase SDRPRINTSPR00080SDRFAMILYcoord: 105..116
score: 46.23
coord: 160..168
score: 48.98
coord: 180..199
score: 43.64
NoneNo IPR availableSMARTSM00822PKS_KRcoord: 20..211
e-value: 0.0043
score: -56.3
NoneNo IPR availablePFAMPF13561adh_short_C2coord: 26..271
e-value: 6.5E-58
score: 196.0
NoneNo IPR availableGENE3D3.40.50.720coord: 10..271
e-value: 2.5E-81
score: 274.9
NoneNo IPR availablePIRSRPIRSR000094-3PIRSR000094-3coord: 17..270
e-value: 6.7E-10
score: 36.4
NoneNo IPR availablePIRSRPIRSR000095-1PIRSR000095-1coord: 23..220
e-value: 1.6E-11
score: 41.8
NoneNo IPR availablePANTHERPTHR48107NADPH-DEPENDENT ALDEHYDE REDUCTASE-LIKE PROTEIN, CHLOROPLASTIC-RELATEDcoord: 10..272
NoneNo IPR availablePANTHERPTHR48107:SF19SHORT-CHAIN DEHYDROGENASE/REDUCTASEcoord: 10..272
IPR020904Short-chain dehydrogenase/reductase, conserved sitePROSITEPS00061ADH_SHORTcoord: 167..195
IPR036291NAD(P)-binding domain superfamilySUPERFAMILY51735NAD(P)-binding Rossmann-fold domainscoord: 18..271

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Moc05g14550.1Moc05g14550.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0005975 carbohydrate metabolic process
biological_process GO:0000724 double-strand break repair via homologous recombination
biological_process GO:0006303 double-strand break repair via nonhomologous end joining
biological_process GO:0042138 meiotic DNA double-strand break formation
biological_process GO:0097552 mitochondrial double-strand break repair via homologous recombination
biological_process GO:0007095 mitotic G2 DNA damage checkpoint signaling
biological_process GO:0000723 telomere maintenance
cellular_component GO:0030870 Mre11 complex
cellular_component GO:0035861 site of double-strand break
molecular_function GO:0008296 3'-5'-exodeoxyribonuclease activity
molecular_function GO:0030246 carbohydrate binding
molecular_function GO:0030145 manganese ion binding
molecular_function GO:0016491 oxidoreductase activity
molecular_function GO:0000014 single-stranded DNA endodeoxyribonuclease activity