Moc05g14520 (gene) Bitter gourd (OHB3-1) v2

Overview
NameMoc05g14520
Typegene
OrganismMomordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
Description(1->3)-beta-glucan endohydrolase
Locationchr5: 10992479 .. 10994681 (+)
RNA-Seq ExpressionMoc05g14520
SyntenyMoc05g14520
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGAAAGAAGATTAGGGTTTCTGAAGTGGGTTTTATTGGTTGGGGTATTGGGTTGCTGTGTTGAAGGTCTTGGGGTTAATTGGGGAACCATGGCCAATCATAGATTGCCGCCTAAGACAGTTGTGCAGATGTTTAAGGATAATGGGATTAAGAAAGTGAAGCTGTTTGATGCAGATCAGTCCACCATGGGTGCTCTGGCTGGGAGTGGTATAGAGGTCATGGTTGCCATTCCCAATGATCAACTTGCCGCCATGGGGGACTACAAGCGAGCTCAGCAATGGGTGCAGAGGAACGTCACTCGCTATAACTTCGATGGAGGAGTTGTCATCAAGTAATTCAATCTACTTTTTTCTTCAAACTATTTCAAACCACAGAAAAATAGATTGTGTTTCCTTGGTTTTTGATTCTAAACTTACTGAAGCCTCTTTTGTTTTTTTTAGTTTTCTTTAATAAGTTATTGGGTCATTTGGATCTTCCAAATCCCCTCCCCCTTTAGATAGTTATGTTATTTCTATATCTGAGGAAGTCAGAAAGTTTGCATAAGTGATCCAATCCATATTTGGATGGTTGTTTGAACATATTTCTTATTGCTATATTTAATTAGCAATCTGAGACACCACGGTTTGAGTTTTTTCCCTTTTTTATTTGAGATGGGTTTTGGAAATGGATTCAAGTTTTTTTTTATTTATTTTTTTTATTTTTTAGTATCGACTCTCATAGAATATTGTATGAATCGTTGGCTCTCCAGTTTCAGAAAAGGTTCGAAACTTATTTAACTATTTAAAACAAGTTGTGGATACGGTTTACTTTGACCAAGAACCCCTTAATGGATTGATCCTATCTGCCACTGGAATTTAGCTGTCCCGTTCTTTATGAAATTTGACAAATTTCTGTTCCTTTAGAAATGATAATGTTAATTATTGTGCATGTCAATTCTCTTCCTTAGATAGATGGGTGTCGTTTTCTTAGTTAATAAATACCAAAGCAAAGATTCTACTGAAAAGTATAGTTTAGTTTGCAATCTCTATGGAAACTAATATTACCTGTTTGGATGGAACTAGTTCTCTTATTATGCTGTTTTTGTACAGATATGTTGCTGTTGGTAATGAACCATTTCTGTCCTCCTATAATGGATCATTCCTGAATGTTACCTTTCCAGCCCTTCAGAACATTCAAAATGCCCTCAATGAAGCTGGTCTTGGAGACTCCATCAAGGCAACGGTGCCCTTAAATGCTGATGTTTACAACTCCCCGGTTAATAGCCCTTATCCATCCGCTGGCAGGTTCCGCAGCGATATTAACGGGCTCATGACCGATATTGTGCAGTTTCTAAGCAAGAACAATGCGCCCTTTACTGTAAACATTTACCCTTTCCTCAGTCTTTATGGCAACGATAACTTCCCTTTCGACTATGCTTTCTTTGATGGGGCAAGTAATCCCGTGGTTGACGCTGGCAATGGGATTCAGTATACGAATGTTTTTGACGCCAACTTCGATACATTGGTTTCGTCTCTGAAAGCTGTTGGACATGGGGACATGCCAATTCTAGTTGGCGAGGTTGGATGGCCAACCGATGGTGACAAGAATGCCAATGTCGGTAATGCTTATAGATTCTACAATGGCCTTCTACCGAGGCTTGCAGCCAGTAAGGGCACCCCGCTGAGGGCTGGATACATTGAAGTATACTTGTTCAGTTTCCTGGATGAAAATGGCAAAAGTATTGCTCCAGGAAATTTCGAGCGACATTGGGGTATTTTTGGATACGATGGCCAGCCAAAGTTCGGCATGGATCTTTCTGGTCAGGGCCAAAACAAACTACTTGTAGGAGCACAGGATGTGCAGTATCTTCCTCGCAAGTGGTGTATGTTCAATCCAAATGCCAAGGATCTCAGTAAACTTGCTGATAACATAAACTACGCCTGCACCTTTTCGGATTGCACTTCCCTCGGCTACGGATCTTCCTGCAATAATTTGGACGCTAACGGGAACGCATCTTATGCATTCAATATGTATTTCCAGGTGCAAAATCAAGATGAAATGGCCTGTAATTTTCAAGGTTTAGCCACGATAACGTCACAAAATCTTTCACAAGGTACTTGCAATTTCATCATTCAGCTAGGTTCTTCCTCGCCCCCGCCCCTTGCTGCTTCATTTGTTAGTTTAGCATCTATGGTCATGTTATTGACAGCATTATTACTATAA

mRNA sequence

ATGGAAAGAAGATTAGGGTTTCTGAAGTGGGTTTTATTGGTTGGGGTATTGGGTTGCTGTGTTGAAGGTCTTGGGGTTAATTGGGGAACCATGGCCAATCATAGATTGCCGCCTAAGACAGTTGTGCAGATGTTTAAGGATAATGGGATTAAGAAAGTGAAGCTGTTTGATGCAGATCAGTCCACCATGGGTGCTCTGGCTGGGAGTGGTATAGAGGTCATGGTTGCCATTCCCAATGATCAACTTGCCGCCATGGGGGACTACAAGCGAGCTCAGCAATGGGTGCAGAGGAACGTCACTCGCTATAACTTCGATGGAGGAGTTGTCATCAAATATGTTGCTGTTGGTAATGAACCATTTCTGTCCTCCTATAATGGATCATTCCTGAATGTTACCTTTCCAGCCCTTCAGAACATTCAAAATGCCCTCAATGAAGCTGGTCTTGGAGACTCCATCAAGGCAACGGTGCCCTTAAATGCTGATGTTTACAACTCCCCGGTTAATAGCCCTTATCCATCCGCTGGCAGGTTCCGCAGCGATATTAACGGGCTCATGACCGATATTGTGCAGTTTCTAAGCAAGAACAATGCGCCCTTTACTGTAAACATTTACCCTTTCCTCAGTCTTTATGGCAACGATAACTTCCCTTTCGACTATGCTTTCTTTGATGGGGCAAGTAATCCCGTGGTTGACGCTGGCAATGGGATTCAGTATACGAATGTTTTTGACGCCAACTTCGATACATTGGTTTCGTCTCTGAAAGCTGTTGGACATGGGGACATGCCAATTCTAGTTGGCGAGGTTGGATGGCCAACCGATGGTGACAAGAATGCCAATGTCGGTAATGCTTATAGATTCTACAATGGCCTTCTACCGAGGCTTGCAGCCAGTAAGGGCACCCCGCTGAGGGCTGGATACATTGAAGTATACTTGTTCAGTTTCCTGGATGAAAATGGCAAAAGTATTGCTCCAGGAAATTTCGAGCGACATTGGGGTATTTTTGGATACGATGGCCAGCCAAAGTTCGGCATGGATCTTTCTGGTCAGGGCCAAAACAAACTACTTGTAGGAGCACAGGATGTGCAGTATCTTCCTCGCAAGTGGTGTATGTTCAATCCAAATGCCAAGGATCTCAGTAAACTTGCTGATAACATAAACTACGCCTGCACCTTTTCGGATTGCACTTCCCTCGGCTACGGATCTTCCTGCAATAATTTGGACGCTAACGGGAACGCATCTTATGCATTCAATATGTATTTCCAGGTGCAAAATCAAGATGAAATGGCCTGTAATTTTCAAGGTTTAGCCACGATAACGTCACAAAATCTTTCACAAGGTACTTGCAATTTCATCATTCAGCTAGGTTCTTCCTCGCCCCCGCCCCTTGCTGCTTCATTTGTTAGTTTAGCATCTATGGTCATGTTATTGACAGCATTATTACTATAA

Coding sequence (CDS)

ATGGAAAGAAGATTAGGGTTTCTGAAGTGGGTTTTATTGGTTGGGGTATTGGGTTGCTGTGTTGAAGGTCTTGGGGTTAATTGGGGAACCATGGCCAATCATAGATTGCCGCCTAAGACAGTTGTGCAGATGTTTAAGGATAATGGGATTAAGAAAGTGAAGCTGTTTGATGCAGATCAGTCCACCATGGGTGCTCTGGCTGGGAGTGGTATAGAGGTCATGGTTGCCATTCCCAATGATCAACTTGCCGCCATGGGGGACTACAAGCGAGCTCAGCAATGGGTGCAGAGGAACGTCACTCGCTATAACTTCGATGGAGGAGTTGTCATCAAATATGTTGCTGTTGGTAATGAACCATTTCTGTCCTCCTATAATGGATCATTCCTGAATGTTACCTTTCCAGCCCTTCAGAACATTCAAAATGCCCTCAATGAAGCTGGTCTTGGAGACTCCATCAAGGCAACGGTGCCCTTAAATGCTGATGTTTACAACTCCCCGGTTAATAGCCCTTATCCATCCGCTGGCAGGTTCCGCAGCGATATTAACGGGCTCATGACCGATATTGTGCAGTTTCTAAGCAAGAACAATGCGCCCTTTACTGTAAACATTTACCCTTTCCTCAGTCTTTATGGCAACGATAACTTCCCTTTCGACTATGCTTTCTTTGATGGGGCAAGTAATCCCGTGGTTGACGCTGGCAATGGGATTCAGTATACGAATGTTTTTGACGCCAACTTCGATACATTGGTTTCGTCTCTGAAAGCTGTTGGACATGGGGACATGCCAATTCTAGTTGGCGAGGTTGGATGGCCAACCGATGGTGACAAGAATGCCAATGTCGGTAATGCTTATAGATTCTACAATGGCCTTCTACCGAGGCTTGCAGCCAGTAAGGGCACCCCGCTGAGGGCTGGATACATTGAAGTATACTTGTTCAGTTTCCTGGATGAAAATGGCAAAAGTATTGCTCCAGGAAATTTCGAGCGACATTGGGGTATTTTTGGATACGATGGCCAGCCAAAGTTCGGCATGGATCTTTCTGGTCAGGGCCAAAACAAACTACTTGTAGGAGCACAGGATGTGCAGTATCTTCCTCGCAAGTGGTGTATGTTCAATCCAAATGCCAAGGATCTCAGTAAACTTGCTGATAACATAAACTACGCCTGCACCTTTTCGGATTGCACTTCCCTCGGCTACGGATCTTCCTGCAATAATTTGGACGCTAACGGGAACGCATCTTATGCATTCAATATGTATTTCCAGGTGCAAAATCAAGATGAAATGGCCTGTAATTTTCAAGGTTTAGCCACGATAACGTCACAAAATCTTTCACAAGGTACTTGCAATTTCATCATTCAGCTAGGTTCTTCCTCGCCCCCGCCCCTTGCTGCTTCATTTGTTAGTTTAGCATCTATGGTCATGTTATTGACAGCATTATTACTATAA

Protein sequence

MERRLGFLKWVLLVGVLGCCVEGLGVNWGTMANHRLPPKTVVQMFKDNGIKKVKLFDADQSTMGALAGSGIEVMVAIPNDQLAAMGDYKRAQQWVQRNVTRYNFDGGVVIKYVAVGNEPFLSSYNGSFLNVTFPALQNIQNALNEAGLGDSIKATVPLNADVYNSPVNSPYPSAGRFRSDINGLMTDIVQFLSKNNAPFTVNIYPFLSLYGNDNFPFDYAFFDGASNPVVDAGNGIQYTNVFDANFDTLVSSLKAVGHGDMPILVGEVGWPTDGDKNANVGNAYRFYNGLLPRLAASKGTPLRAGYIEVYLFSFLDENGKSIAPGNFERHWGIFGYDGQPKFGMDLSGQGQNKLLVGAQDVQYLPRKWCMFNPNAKDLSKLADNINYACTFSDCTSLGYGSSCNNLDANGNASYAFNMYFQVQNQDEMACNFQGLATITSQNLSQGTCNFIIQLGSSSPPPLAASFVSLASMVMLLTALLL
Homology
BLAST of Moc05g14520 vs. NCBI nr
Match: XP_022155900.1 (glucan endo-1,3-beta-glucosidase 8-like [Momordica charantia])

HSP 1 Score: 980.7 bits (2534), Expect = 4.5e-282
Identity = 480/481 (99.79%), Postives = 480/481 (99.79%), Query Frame = 0

Query: 1   MERRLGFLKWVLLVGVLGCCVEGLGVNWGTMANHRLPPKTVVQMFKDNGIKKVKLFDADQ 60
           MERRLGFLKWVLLVGVLGCCVEGLGVNWGTMANHRLPPKTVVQMFKDNGIKKVKLFDADQ
Sbjct: 1   MERRLGFLKWVLLVGVLGCCVEGLGVNWGTMANHRLPPKTVVQMFKDNGIKKVKLFDADQ 60

Query: 61  STMGALAGSGIEVMVAIPNDQLAAMGDYKRAQQWVQRNVTRYNFDGGVVIKYVAVGNEPF 120
           STMGALAGSGIEVMVAIPNDQLAAMGDYKRAQQWVQRNVTRYNFDGGVVIKYVAVGNEPF
Sbjct: 61  STMGALAGSGIEVMVAIPNDQLAAMGDYKRAQQWVQRNVTRYNFDGGVVIKYVAVGNEPF 120

Query: 121 LSSYNGSFLNVTFPALQNIQNALNEAGLGDSIKATVPLNADVYNSPVNSPYPSAGRFRSD 180
           LSSYNGSFLNVTFPALQNIQNALNEAGLGDSIKATVPLNADVYNSPVNSPYPSAGRFRSD
Sbjct: 121 LSSYNGSFLNVTFPALQNIQNALNEAGLGDSIKATVPLNADVYNSPVNSPYPSAGRFRSD 180

Query: 181 INGLMTDIVQFLSKNNAPFTVNIYPFLSLYGNDNFPFDYAFFDGASNPVVDAGNGIQYTN 240
           INGLMTDIVQFLSKNNAPFTVNIYPFLSLYGNDNFPFDYAFFDGASNPVVDAGNGIQYTN
Sbjct: 181 INGLMTDIVQFLSKNNAPFTVNIYPFLSLYGNDNFPFDYAFFDGASNPVVDAGNGIQYTN 240

Query: 241 VFDANFDTLVSSLKAVGHGDMPILVGEVGWPTDGDKNANVGNAYRFYNGLLPRLAASKGT 300
           VFDANFDTLVSSLKAVGHGDMPILVGEVGWPTDGDKNANVGNAYRFYNGLLPRLAASKGT
Sbjct: 241 VFDANFDTLVSSLKAVGHGDMPILVGEVGWPTDGDKNANVGNAYRFYNGLLPRLAASKGT 300

Query: 301 PLRAGYIEVYLFSFLDENGKSIAPGNFERHWGIFGYDGQPKFGMDLSGQGQNKLLVGAQD 360
           PLRAGYIEVYLFSFLDENGKSIAPGNFERHWGIFGYDGQPKFGMDLSGQGQNKLLVGAQD
Sbjct: 301 PLRAGYIEVYLFSFLDENGKSIAPGNFERHWGIFGYDGQPKFGMDLSGQGQNKLLVGAQD 360

Query: 361 VQYLPRKWCMFNPNAKDLSKLADNINYACTFSDCTSLGYGSSCNNLDANGNASYAFNMYF 420
           VQYLPRKWCMFNPNAKDLSKLADNINYACTFSDCTSLGYGSSCNNLDANGNASYAFNMYF
Sbjct: 361 VQYLPRKWCMFNPNAKDLSKLADNINYACTFSDCTSLGYGSSCNNLDANGNASYAFNMYF 420

Query: 421 QVQNQDEMACNFQGLATITSQNLSQGTCNFIIQLGSSSPPPLAASFVSLASMVMLLTALL 480
           QVQNQDEMACNFQGLATITSQNLSQGTCNFIIQLGSSSPPPLAASFVSLASMVMLLTAL 
Sbjct: 421 QVQNQDEMACNFQGLATITSQNLSQGTCNFIIQLGSSSPPPLAASFVSLASMVMLLTALX 480

Query: 481 L 482
           L
Sbjct: 481 L 481

BLAST of Moc05g14520 vs. NCBI nr
Match: XP_038903901.1 (glucan endo-1,3-beta-glucosidase 8-like [Benincasa hispida])

HSP 1 Score: 908.3 bits (2346), Expect = 2.8e-260
Identity = 445/478 (93.10%), Postives = 460/478 (96.23%), Query Frame = 0

Query: 4   RLGFLKWVLLVGVLGCCVEGLGVNWGTMANHRLPPKTVVQMFKDNGIKKVKLFDADQSTM 63
           RLGFLKWVLLVGVLGC VEGLGVNWGTMANHRLPPKT+VQMFKDNGIKKVKLFDADQS+M
Sbjct: 3   RLGFLKWVLLVGVLGCYVEGLGVNWGTMANHRLPPKTIVQMFKDNGIKKVKLFDADQSSM 62

Query: 64  GALAGSGIEVMVAIPNDQLAAMGDYKRAQQWVQRNVTRYNFDGGVVIKYVAVGNEPFLSS 123
           GALAG+GIEVMVAIPNDQL+AMGDY RA+QWVQRNV+RY FDGGV IKYVAVGNEPFLSS
Sbjct: 63  GALAGTGIEVMVAIPNDQLSAMGDYNRAKQWVQRNVSRYTFDGGVTIKYVAVGNEPFLSS 122

Query: 124 YNGSFLNVTFPALQNIQNALNEAGLGDSIKATVPLNADVYNSPVNSPYPSAGRFRSDING 183
           YNGSFLNVTFPALQNIQNALNEAG GDSIKATVPLNADVYNSPVNSPYPSAGRFRSDING
Sbjct: 123 YNGSFLNVTFPALQNIQNALNEAGHGDSIKATVPLNADVYNSPVNSPYPSAGRFRSDING 182

Query: 184 LMTDIVQFLSKNNAPFTVNIYPFLSLYGNDNFPFDYAFFDGASNPVVDAGNGIQYTNVFD 243
           LMTDIVQFLSKNNAPFTVNIYPFLSLYGNDNFPFDYAFFDGASNPVVD G GIQYTNVFD
Sbjct: 183 LMTDIVQFLSKNNAPFTVNIYPFLSLYGNDNFPFDYAFFDGASNPVVDVGTGIQYTNVFD 242

Query: 244 ANFDTLVSSLKAVGHGDMPILVGEVGWPTDGDKNANVGNAYRFYNGLLPRLAASKGTPLR 303
           ANFDTLVSSLKAV  GDMPILVGEVGWPTDGDKNAN GNAYRFYNGLLPRLAA+KGTPLR
Sbjct: 243 ANFDTLVSSLKAVELGDMPILVGEVGWPTDGDKNANAGNAYRFYNGLLPRLAANKGTPLR 302

Query: 304 AGYIEVYLFSFLDENGKSIAPGNFERHWGIFGYDGQPKFGMDLSGQGQNKLLVGAQDVQY 363
            G+IEVYLFSFLDENGKSIAPGNFERHWGIFGYDGQPKF MDLSGQGQNKLLVGAQ+VQY
Sbjct: 303 PGFIEVYLFSFLDENGKSIAPGNFERHWGIFGYDGQPKFAMDLSGQGQNKLLVGAQNVQY 362

Query: 364 LPRKWCMFNPNAKDLSKLADNINYACTFSDCTSLGYGSSCNNLDANGNASYAFNMYFQVQ 423
           LP+KWCMFNPNAKDLSKLADNI+YACTFSDCTSLGYGSSCNNLDANGNASYAFNMYFQVQ
Sbjct: 363 LPKKWCMFNPNAKDLSKLADNIDYACTFSDCTSLGYGSSCNNLDANGNASYAFNMYFQVQ 422

Query: 424 NQDEMACNFQGLATITSQNLSQGTCNFIIQLGSSSPPPLAASFVSLASMVMLLTALLL 482
           NQDE+ACNFQGLAT+TSQNLSQGTCNFIIQL +SSP   AASFVSLASM +L TALLL
Sbjct: 423 NQDELACNFQGLATMTSQNLSQGTCNFIIQL-ASSPSSQAASFVSLASMALLFTALLL 479

BLAST of Moc05g14520 vs. NCBI nr
Match: XP_004141142.1 (glucan endo-1,3-beta-glucosidase 8 [Cucumis sativus] >KGN59764.1 hypothetical protein Csa_001412 [Cucumis sativus])

HSP 1 Score: 903.7 bits (2334), Expect = 7.0e-259
Identity = 442/477 (92.66%), Postives = 459/477 (96.23%), Query Frame = 0

Query: 5   LGFLKWVLLVGVLGCCVEGLGVNWGTMANHRLPPKTVVQMFKDNGIKKVKLFDADQSTMG 64
           LGFLKWVLLVGVLG CVEGLGVNWGTMANHRLPPKTVVQMFKDNGI+KVKLFDADQS+MG
Sbjct: 4   LGFLKWVLLVGVLGSCVEGLGVNWGTMANHRLPPKTVVQMFKDNGIQKVKLFDADQSSMG 63

Query: 65  ALAGSGIEVMVAIPNDQLAAMGDYKRAQQWVQRNVTRYNFDGGVVIKYVAVGNEPFLSSY 124
           ALAG+GIEVMVAIPNDQL+AMGDY RA+QWVQRNVTRY FDGGV IKYVAVGNEPFLSSY
Sbjct: 64  ALAGTGIEVMVAIPNDQLSAMGDYNRAKQWVQRNVTRYTFDGGVTIKYVAVGNEPFLSSY 123

Query: 125 NGSFLNVTFPALQNIQNALNEAGLGDSIKATVPLNADVYNSPVNSPYPSAGRFRSDINGL 184
           NGSFLNVTFPAL NIQNALNEAG GDSIKATVPLNADVYNSPVNSPYPSAGRFR+DIN L
Sbjct: 124 NGSFLNVTFPALLNIQNALNEAGHGDSIKATVPLNADVYNSPVNSPYPSAGRFRNDINQL 183

Query: 185 MTDIVQFLSKNNAPFTVNIYPFLSLYGNDNFPFDYAFFDGASNPVVDAGNGIQYTNVFDA 244
           MTDIVQFL+KN APFTVNIYPFLSLYGNDNFPFDYAFFDGASNPVVD G GIQYTNVFDA
Sbjct: 184 MTDIVQFLNKNKAPFTVNIYPFLSLYGNDNFPFDYAFFDGASNPVVDIGTGIQYTNVFDA 243

Query: 245 NFDTLVSSLKAVGHGDMPILVGEVGWPTDGDKNANVGNAYRFYNGLLPRLAASKGTPLRA 304
           NFDTLVSSLKAVG GDMPILVGEVGWPTDGDKN NVGNAYRFYNGLLPRLAA+KGTPLR 
Sbjct: 244 NFDTLVSSLKAVGLGDMPILVGEVGWPTDGDKNGNVGNAYRFYNGLLPRLAANKGTPLRP 303

Query: 305 GYIEVYLFSFLDENGKSIAPGNFERHWGIFGYDGQPKFGMDLSGQGQNKLLVGAQDVQYL 364
           G+IEVYLFSFLDENGKSIAPGNFERHWGIFGYDGQPKFGMDLSGQGQNKLLVGAQ+VQYL
Sbjct: 304 GFIEVYLFSFLDENGKSIAPGNFERHWGIFGYDGQPKFGMDLSGQGQNKLLVGAQNVQYL 363

Query: 365 PRKWCMFNPNAKDLSKLADNINYACTFSDCTSLGYGSSCNNLDANGNASYAFNMYFQVQN 424
           P+KWCMFNPNAKDLSKLADNI+YACTFSDCTSLGYGSSCNNLDANGNASYAFNMYFQVQN
Sbjct: 364 PQKWCMFNPNAKDLSKLADNIDYACTFSDCTSLGYGSSCNNLDANGNASYAFNMYFQVQN 423

Query: 425 QDEMACNFQGLATITSQNLSQGTCNFIIQLGSSSPPPLAASFVSLASMVMLLTALLL 482
           QDE+ACNFQGLAT+TSQNLSQGTCNFIIQL +SSPP  AASFVSLASM++L  ALLL
Sbjct: 424 QDELACNFQGLATVTSQNLSQGTCNFIIQL-ASSPPSHAASFVSLASMILLFIALLL 479

BLAST of Moc05g14520 vs. NCBI nr
Match: XP_008465328.1 (PREDICTED: glucan endo-1,3-beta-glucosidase 8-like [Cucumis melo] >KAA0051277.1 glucan endo-1,3-beta-glucosidase 8-like [Cucumis melo var. makuwa] >TYK29984.1 glucan endo-1,3-beta-glucosidase 8-like [Cucumis melo var. makuwa])

HSP 1 Score: 901.0 bits (2327), Expect = 4.5e-258
Identity = 443/478 (92.68%), Postives = 459/478 (96.03%), Query Frame = 0

Query: 4   RLGFLKWVLLVGVLGCCVEGLGVNWGTMANHRLPPKTVVQMFKDNGIKKVKLFDADQSTM 63
           RLGFLKWVLLVGVLG CVEGLGVNWGTMANHRLPPKTVVQM KDNGI+KVKLFDADQS+M
Sbjct: 3   RLGFLKWVLLVGVLGSCVEGLGVNWGTMANHRLPPKTVVQMLKDNGIQKVKLFDADQSSM 62

Query: 64  GALAGSGIEVMVAIPNDQLAAMGDYKRAQQWVQRNVTRYNFDGGVVIKYVAVGNEPFLSS 123
           GALAG+GIEVMVAIPNDQL+AMGDY RA+QWVQRNVTRY FDGGV IKYVAVGNEPFLSS
Sbjct: 63  GALAGTGIEVMVAIPNDQLSAMGDYNRAKQWVQRNVTRYIFDGGVTIKYVAVGNEPFLSS 122

Query: 124 YNGSFLNVTFPALQNIQNALNEAGLGDSIKATVPLNADVYNSPVNSPYPSAGRFRSDING 183
           YNGSFLNVTFPALQNIQNALNEAG GDSIKATVPLNADVYNSPVNSPYPSAGRFRSDIN 
Sbjct: 123 YNGSFLNVTFPALQNIQNALNEAGHGDSIKATVPLNADVYNSPVNSPYPSAGRFRSDINQ 182

Query: 184 LMTDIVQFLSKNNAPFTVNIYPFLSLYGNDNFPFDYAFFDGASNPVVDAGNGIQYTNVFD 243
           LM DIVQFL+KN APFTVNIYPFLSLYGNDNFPFDYAFFDGASNPVVD G GIQYTNVFD
Sbjct: 183 LMNDIVQFLNKNKAPFTVNIYPFLSLYGNDNFPFDYAFFDGASNPVVDIGTGIQYTNVFD 242

Query: 244 ANFDTLVSSLKAVGHGDMPILVGEVGWPTDGDKNANVGNAYRFYNGLLPRLAASKGTPLR 303
           ANFDTLVSSLKAVG GDMPILVGEVGWPTDGDKNAN GNAYRFYNGLLPRLAA+KGTPLR
Sbjct: 243 ANFDTLVSSLKAVGLGDMPILVGEVGWPTDGDKNANDGNAYRFYNGLLPRLAANKGTPLR 302

Query: 304 AGYIEVYLFSFLDENGKSIAPGNFERHWGIFGYDGQPKFGMDLSGQGQNKLLVGAQDVQY 363
            G+IEVYLFSFLDENGKSIAPGNFERHWGIFGYDGQPKFGMDLSGQGQNKLLVGAQ+V+Y
Sbjct: 303 PGFIEVYLFSFLDENGKSIAPGNFERHWGIFGYDGQPKFGMDLSGQGQNKLLVGAQNVEY 362

Query: 364 LPRKWCMFNPNAKDLSKLADNINYACTFSDCTSLGYGSSCNNLDANGNASYAFNMYFQVQ 423
           LP+KWCMFNPNAKDLSKLADNI+YACTFSDCTSLGYGSSCNNLDANGNASYAFNMYFQVQ
Sbjct: 363 LPQKWCMFNPNAKDLSKLADNIDYACTFSDCTSLGYGSSCNNLDANGNASYAFNMYFQVQ 422

Query: 424 NQDEMACNFQGLATITSQNLSQGTCNFIIQLGSSSPPPLAASFVSLASMVMLLTALLL 482
           NQDE+ACNFQGLATITS+NLSQGTCNFIIQL +SSPP  AASFVSLASMV+L  ALLL
Sbjct: 423 NQDELACNFQGLATITSRNLSQGTCNFIIQL-ASSPPSHAASFVSLASMVLLFIALLL 479

BLAST of Moc05g14520 vs. NCBI nr
Match: KAG6581686.1 (Glucan endo-1,3-beta-glucosidase 8, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 900.6 bits (2326), Expect = 5.9e-258
Identity = 439/478 (91.84%), Postives = 455/478 (95.19%), Query Frame = 0

Query: 4   RLGFLKWVLLVGVLGCCVEGLGVNWGTMANHRLPPKTVVQMFKDNGIKKVKLFDADQSTM 63
           RL  LKWVLLVGVLGCC+EGLGVNWGTMANH+LPPKTVVQMFKDNGI+KVKLFDADQS+M
Sbjct: 3   RLWILKWVLLVGVLGCCIEGLGVNWGTMANHKLPPKTVVQMFKDNGIQKVKLFDADQSSM 62

Query: 64  GALAGSGIEVMVAIPNDQLAAMGDYKRAQQWVQRNVTRYNFDGGVVIKYVAVGNEPFLSS 123
           GALAG+GIEVMVAIPNDQL AMGDY RA+QWVQRNV+RY FDGGV IKYVAVGNEPFLSS
Sbjct: 63  GALAGTGIEVMVAIPNDQLVAMGDYNRAKQWVQRNVSRYTFDGGVTIKYVAVGNEPFLSS 122

Query: 124 YNGSFLNVTFPALQNIQNALNEAGLGDSIKATVPLNADVYNSPVNSPYPSAGRFRSDING 183
           YNGSFLNVTFPALQNIQNALNEAG GD+IKATVPLNADVYNSPVN+PYPSAGRFRSDING
Sbjct: 123 YNGSFLNVTFPALQNIQNALNEAGYGDTIKATVPLNADVYNSPVNTPYPSAGRFRSDING 182

Query: 184 LMTDIVQFLSKNNAPFTVNIYPFLSLYGNDNFPFDYAFFDGASNPVVDAGNGIQYTNVFD 243
           LMT IVQFLSKNNAPFTVNIYPFLSLYGNDNFPFDYAFFDGASNPVVD   GIQYTNVFD
Sbjct: 183 LMTQIVQFLSKNNAPFTVNIYPFLSLYGNDNFPFDYAFFDGASNPVVDMNTGIQYTNVFD 242

Query: 244 ANFDTLVSSLKAVGHGDMPILVGEVGWPTDGDKNANVGNAYRFYNGLLPRLAASKGTPLR 303
           ANFDTLVSSLKAVG GDMPILVGEVGWPTDGDKNAN GNAYRFYNGLLPRLAA+KGTPLR
Sbjct: 243 ANFDTLVSSLKAVGLGDMPILVGEVGWPTDGDKNANAGNAYRFYNGLLPRLAANKGTPLR 302

Query: 304 AGYIEVYLFSFLDENGKSIAPGNFERHWGIFGYDGQPKFGMDLSGQGQNKLLVGAQDVQY 363
            G+IEVYLFSFLDENGKSIAPGNFERHWGIFGYDGQPKFGMDLSGQGQNKLLVGAQDVQY
Sbjct: 303 PGFIEVYLFSFLDENGKSIAPGNFERHWGIFGYDGQPKFGMDLSGQGQNKLLVGAQDVQY 362

Query: 364 LPRKWCMFNPNAKDLSKLADNINYACTFSDCTSLGYGSSCNNLDANGNASYAFNMYFQVQ 423
           LP+KWCMFNPNAKDLSKLADNI+YACTFSDCTSLGYGSSCNNLDANGNASYAFNMYFQVQ
Sbjct: 363 LPQKWCMFNPNAKDLSKLADNIDYACTFSDCTSLGYGSSCNNLDANGNASYAFNMYFQVQ 422

Query: 424 NQDEMACNFQGLATITSQNLSQGTCNFIIQLGSSSPPPLAASFVSLASMVMLLTALLL 482
           NQDE+ACNFQGLATITSQNLSQGTC FIIQL SSS P L ASFVSL SM +L TA+ L
Sbjct: 423 NQDELACNFQGLATITSQNLSQGTCKFIIQLASSS-PSLDASFVSLVSMALLFTAMWL 479

BLAST of Moc05g14520 vs. ExPASy Swiss-Prot
Match: Q6NKW9 (Glucan endo-1,3-beta-glucosidase 8 OS=Arabidopsis thaliana OX=3702 GN=At1g64760 PE=2 SV=2)

HSP 1 Score: 730.7 bits (1885), Expect = 1.1e-209
Identity = 352/465 (75.70%), Postives = 399/465 (85.81%), Query Frame = 0

Query: 10  WVLLVGVLGCCVEGLGVNWGTMANHRLPPKTVVQMFKDNGIKKVKLFDADQSTMGALAGS 69
           +V+++G LG  V GLGVNWGTMA H+LPPKTVVQM KDN I KVKLFDAD++TMGALAGS
Sbjct: 11  FVIVIGHLGILVNGLGVNWGTMATHKLPPKTVVQMLKDNNINKVKLFDADETTMGALAGS 70

Query: 70  GIEVMVAIPNDQLAAMGDYKRAQQWVQRNVTRYNFDGGVVIKYVAVGNEPFLSSYNGSFL 129
           G+EVMVAIPNDQL  M  Y RA+ WV++NVTRYNFDGGV I +VAVGNEPFL SYNGSF+
Sbjct: 71  GLEVMVAIPNDQLKVMTSYDRAKDWVRKNVTRYNFDGGVNITFVAVGNEPFLKSYNGSFI 130

Query: 130 NVTFPALQNIQNALNEAGLGDSIKATVPLNADVYNSPVNSPYPSAGRFRSDINGLMTDIV 189
           N+TFPAL NIQNALNEAGLG+S+KATVPLNADVY+SP ++P PSAGRFR DI G MT IV
Sbjct: 131 NLTFPALANIQNALNEAGLGNSVKATVPLNADVYDSPASNPVPSAGRFRPDIIGQMTQIV 190

Query: 190 QFLSKNNAPFTVNIYPFLSLYGNDNFPFDYAFFDGASNPVVDAGNGIQYTNVFDANFDTL 249
            FL KNNAP T+NIYPFLSLYGND+FP +YAFFDGA  P+ D  NGI YTNVFDANFDTL
Sbjct: 191 DFLGKNNAPITINIYPFLSLYGNDDFPLNYAFFDGA-EPIND--NGIDYTNVFDANFDTL 250

Query: 250 VSSLKAVGHGDMPILVGEVGWPTDGDKNANVGNAYRFYNGLLPRLAASKGTPLRAGYIEV 309
           VSSLKAVGHGDMPI+VGEVGWPT+GDK+AN G+AYRFYNGLLPRL  +KGTPLR  YIEV
Sbjct: 251 VSSLKAVGHGDMPIIVGEVGWPTEGDKHANAGSAYRFYNGLLPRLGTNKGTPLRPTYIEV 310

Query: 310 YLFSFLDENGKSIAPGNFERHWGIFGYDGQPKFGMDLSGQGQNKLLVGAQDVQYLPRKWC 369
           YLF  LDE+ KSIAPG FERHWGIF +DGQPKF +DLSGQGQ+K L+GAQ+V YLP KWC
Sbjct: 311 YLFGLLDEDAKSIAPGPFERHWGIFKFDGQPKFPIDLSGQGQSKFLIGAQNVPYLPNKWC 370

Query: 370 MFNPNAKDLSKLADNINYACTFSDCTSLGYGSSCNNLDANGNASYAFNMYFQVQNQDEMA 429
            FNP AKDL+KLA NI+YACTFSDCT+LGYGSSCN LDANGNASYAFNM+FQV+NQDE A
Sbjct: 371 TFNPEAKDLTKLAANIDYACTFSDCTALGYGSSCNTLDANGNASYAFNMFFQVKNQDESA 430

Query: 430 CNFQGLATITSQNLSQGTCNFIIQLGSSSPPPLAASFVSLASMVM 475
           C FQGLATIT+QN+SQG CNF IQ+ +SS    + S  SL  +++
Sbjct: 431 CYFQGLATITTQNISQGQCNFPIQIVASSASSFSCSSYSLVVLIV 472

BLAST of Moc05g14520 vs. ExPASy Swiss-Prot
Match: Q9M088 (Glucan endo-1,3-beta-glucosidase 5 OS=Arabidopsis thaliana OX=3702 GN=At4g31140 PE=2 SV=1)

HSP 1 Score: 485.7 bits (1249), Expect = 5.9e-136
Identity = 252/479 (52.61%), Postives = 333/479 (69.52%), Query Frame = 0

Query: 7   FLKWVLLVGVLGCCVEGLGVNWGTMANHRLPPKTVVQMFKDNGIKKVKLFDADQSTMGAL 66
           F    LL   L   VEG+GVNWG+ A H LPP TVV++ ++NGI+KVKLF+AD + + AL
Sbjct: 10  FFVITLLYASLLIEVEGIGVNWGSQARHPLPPATVVRLLRENGIQKVKLFEADSAILKAL 69

Query: 67  AGSGIEVMVAIPNDQLAAM-GDYKRAQQWVQRNVTRYNFDGGVVIKYVAVGNEPFLSSYN 126
           + +GI+VMV IPND LA + G    A++WV +NV+ +    GV I+YVAVGNEPFL ++N
Sbjct: 70  SRTGIQVMVGIPNDLLAPLAGSVAAAERWVSQNVSAHVSSNGVDIRYVAVGNEPFLKAFN 129

Query: 127 GSFLNVTFPALQNIQNALNEAGLGDSIKATVPLNADVYNSPVNSPYPSAGRFRSDINGLM 186
           G+F  +T PALQNIQ+A+ +AGL   +K TVPLNADVY S  N   PS G FR +I  LM
Sbjct: 130 GTFEGITLPALQNIQSAIIKAGLATQVKVTVPLNADVYQSASN--LPSDGDFRPEIRDLM 189

Query: 187 TDIVQFLSKNNAPFTVNIYPFLSLYGNDNFPFDYAFFDGASNPVVDAGNGIQYTNVFDAN 246
            +IV+FLS N APFT+NIYPF+SLY + NFP ++AFFDG   P+ D  NG  Y NV DAN
Sbjct: 190 LNIVKFLSDNGAPFTINIYPFISLYNDPNFPVEFAFFDGTGTPIND--NGRIYDNVLDAN 249

Query: 247 FDTLVSSLKAVGHGDMPILVGEVGWPTDGDKNANVGNAYRFYNGLLPRLAASKGTPLRAG 306
           +DTLV SL+  G G++ I+VGEVGWPTDGDKNAN+  A R+  G + R  A+KGTP+R G
Sbjct: 250 YDTLVWSLQKNGFGNLTIIVGEVGWPTDGDKNANLMYARRYNQGFMNRQKANKGTPMRPG 309

Query: 307 YIEVYLFSFLDENGKSIAPGNFERHWGIFGYDGQPKFGMDL-SGQGQNKLLVGAQDVQYL 366
            ++ YLF  +DE+ KSI PGNFERHWGIF  DGQPK+ + L SG G    L+ A+DV YL
Sbjct: 310 AMDAYLFGLIDEDAKSIQPGNFERHWGIFYIDGQPKYQLSLGSGNG----LIPAKDVHYL 369

Query: 367 PRKWCMFNPNAK-DLSKLADNINYACTFSDCTSLGYGSSCNNLDANGNASYAFNMYFQVQ 426
            +KWC+  PNA     +L  +++YAC  +DCTSLGYGSSC NL+   N SYAFN Y+QV 
Sbjct: 370 AKKWCILAPNANLQDPQLGPSVSYACDHADCTSLGYGSSCGNLNLAQNVSYAFNSYYQVS 429

Query: 427 NQDEMACNFQGLATITSQNLSQGTCNFIIQLGSSSPPPLAASF-VSLASMVMLLTALLL 482
           NQ + AC F GL+ +++++ S G+C F I + S      +A   ++ ++ V+LL ++ L
Sbjct: 430 NQLDSACKFPGLSIVSTRDPSVGSCKFKIMIKSEDASEASAMMPITRSTAVLLLLSICL 480

BLAST of Moc05g14520 vs. ExPASy Swiss-Prot
Match: Q93Z08 (Glucan endo-1,3-beta-glucosidase 6 OS=Arabidopsis thaliana OX=3702 GN=At5g58090 PE=2 SV=2)

HSP 1 Score: 480.7 bits (1236), Expect = 1.9e-134
Identity = 237/454 (52.20%), Postives = 319/454 (70.26%), Query Frame = 0

Query: 5   LGFLKWVLLVGVLGCC--VEGLGVNWGTMANHRLPPKTVVQMFKDNGIKKVKLFDADQST 64
           +G+   +LL+ V   C     +G NWGT A+H LPP  VV+M ++NGI+KVKLFDA+  T
Sbjct: 1   MGWGSVLLLLAVALLCQRASSIGANWGTQASHPLPPDIVVRMLRENGIQKVKLFDAEYDT 60

Query: 65  MGALAGSGIEVMVAIPNDQLAAM-GDYKRAQQWVQRNVTRYNFDGGVVIKYVAVGNEPFL 124
           + AL  SGIEVMV IPN+ LA +    K A++WV +NV+ +     V I+YVAVGNEPFL
Sbjct: 61  LRALGKSGIEVMVGIPNEMLATLASSLKAAEKWVAKNVSTHISTDNVNIRYVAVGNEPFL 120

Query: 125 SSYNGSFLNVTFPALQNIQNALNEAGLGDSIKATVPLNADVYNSPVNSPYPSAGRFRSDI 184
           S+YNGS+L+ TFPAL+NIQ A+ +AGL + +K T PLNADVY+S  ++ +PS G FR++I
Sbjct: 121 STYNGSYLSTTFPALRNIQIAIIKAGLQNQVKVTCPLNADVYDS--STTFPSGGDFRANI 180

Query: 185 NGLMTDIVQFLSKNNAPFTVNIYPFLSLYGNDNFPFDYAFFDGASNPVVDAGNGIQYTNV 244
             LM  IV+FLS+N  PFTVNIYP++SLY N +FP DYAFFDG + P+ D G    Y N+
Sbjct: 181 RDLMITIVKFLSENGGPFTVNIYPYISLYTNPDFPVDYAFFDGNAQPLNDGGT--FYYNM 240

Query: 245 FDANFDTLVSSLKAVGHGDMPILVGEVGWPTDGDKNANVGNAYRFYNGLLPRLAASKGTP 304
           FDAN+DTLV +L+  G G+MPI++GE+GWPTDGD NAN+  A +F  G +  ++  KGTP
Sbjct: 241 FDANYDTLVHALEKNGFGNMPIIIGEIGWPTDGDSNANLDYAKKFNQGFMAHISGGKGTP 300

Query: 305 LRAGYIEVYLFSFLDENGKSIAPGNFERHWGIFGYDGQPKFGMDLSGQGQNKLLVGAQDV 364
            R G I+ YLFS +DE+ KS+ PG FERHWGIF +DG PK+ ++L G      L+ A+ V
Sbjct: 301 RRPGPIDAYLFSLIDEDAKSVQPGYFERHWGIFTFDGLPKYALNL-GTTNTGALIQAKGV 360

Query: 365 QYLPRKWCMFNPNAK-DLSKLADNINYACTFSDCTSLGYGSSCNNLDANGNASYAFNMYF 424
           +YL RKWC+  PN + D  ++A  ++YAC+  DCTSLG G+SC NLD   N SYAFN Y+
Sbjct: 361 RYLERKWCVMKPNVRLDDPQVAPAVSYACSLGDCTSLGVGTSCANLDGKQNISYAFNSYY 420

Query: 425 QVQNQDEMACNFQGLATITSQNLSQGTCNFIIQL 455
           Q+Q+Q + AC F  ++ +T  + S GTC F I +
Sbjct: 421 QIQDQLDTACKFPNISEVTKTDPSTGTCRFPIMI 449

BLAST of Moc05g14520 vs. ExPASy Swiss-Prot
Match: Q9FGH4 (Glucan endo-1,3-beta-glucosidase 9 OS=Arabidopsis thaliana OX=3702 GN=At5g58480 PE=2 SV=1)

HSP 1 Score: 357.8 bits (917), Expect = 1.9e-97
Identity = 203/480 (42.29%), Postives = 280/480 (58.33%), Query Frame = 0

Query: 1   MERRLGFLKWVLLVG--VLGCCVEGLGVNWGTMANHRLPPKTVVQMFKDNGIKKVKLFDA 60
           M RRL  L   +  G  + G  V  +G+NWGT A+H LPP  VV++ K NGI KVKLFDA
Sbjct: 1   MARRLFLLLLAVTAGLSLTGTTVRAVGINWGTEASHPLPPSKVVELLKSNGIVKVKLFDA 60

Query: 61  DQSTMGALAGSGIEVMVAIPNDQLAAM-GDYKRAQQWVQRNVTRYNFDGG--VVIKYVAV 120
           D   + AL+GS I V + I N  L ++    K A+ WV  NVTRY F+GG  V I+YVAV
Sbjct: 61  DPKVLRALSGSNIGVTIGIQNSMLKSLNASVKVAESWVHDNVTRY-FNGGNRVRIEYVAV 120

Query: 121 GNEPFLSSYNGSFLNVTFPALQNIQNALNEAGLGDSIKATVPLNADVYNSPVNSPYPSAG 180
           G EPFL SY   +      A  NIQNAL +A L + +K  VP + D + S   S  PS+G
Sbjct: 121 GEEPFLQSYGNQYKPFVIGAAMNIQNALVKANLANEVKVVVPSSFDSFLS--ESGRPSSG 180

Query: 181 RFRSDINGLMTDIVQFLSKNNAPFTVNIYPFLSLYGNDNFPFDYAFFDGASNPVVDAGNG 240
            FR+D+N  M +++ FL+K+++PF V I PFLS + N N   D++ F   +    D    
Sbjct: 181 HFRADLNKTMIELLSFLTKHHSPFFVTISPFLSFHQNKNISLDFSLFKETAKAHKDGRK- 240

Query: 241 IQYTNVFDANFDTLVSSLKAVGHGDMPILVGEVGWPTDGDKNANVGNAYRFYNGLLPRLA 300
             Y N FD ++DTLVS+L  +G  ++ I+V ++GWPTDG +NA    A  F+ GL+  L 
Sbjct: 241 -TYRNSFDLSYDTLVSALFTIGFSEVDIVVSKIGWPTDGAENATSLTAEAFFKGLIVHL- 300

Query: 301 ASKGTPLRAGYIEVYLFSFLDENGKSIAPGNFERHWGIFGYDGQPKFGMDLSGQGQNKLL 360
             K   L    +E Y+ S LDE+ ++++ GNFERHWG+F +DGQ K+    + + Q    
Sbjct: 301 EKKTASLPRPPVETYIESLLDEDQRNLSAGNFERHWGVFTFDGQAKYNFSFNHKNQ---- 360

Query: 361 VGAQDVQYLPRKWCMFNPNAKDLSKLADNINYACTFSDCTSLGYGSSCNNLDANGNASYA 420
           V AQ+VQYLP KWC+ N N KDLS  +     AC  +DCTS+  G SC+ +   GN SYA
Sbjct: 361 VNAQNVQYLPPKWCVVN-NNKDLSNASARALEACAVADCTSILPGGSCSGIRWPGNVSYA 420

Query: 421 FNMYFQVQNQDEMACNFQGLATITSQNLSQGTCNFIIQLGSSSPPPLAASFVSLASMVML 476
           FN  +Q  +    +CNF GL  IT+ + S+  C F IQL +S       +F     +++L
Sbjct: 421 FNSLYQQNDHSAESCNFGGLGLITTVDPSEDNCRFSIQLDTSHSSSQTPNFFQSWPLLLL 469

BLAST of Moc05g14520 vs. ExPASy Swiss-Prot
Match: Q94G86 (Glucan endo-1,3-beta-D-glucosidase OS=Olea europaea OX=4146 GN=OLE9 PE=1 SV=1)

HSP 1 Score: 224.9 bits (572), Expect = 1.9e-57
Identity = 147/436 (33.72%), Postives = 227/436 (52.06%), Query Frame = 0

Query: 24  LGVNWGTMANHRLPPKTVVQMFKDNGIKKVKLFDADQSTMGALAGSGIEVMVAIPNDQLA 83
           LGVN+G ++++    +  V + K   I+KV+LF A+ + + A A +G+E+++   N  + 
Sbjct: 30  LGVNYGQLSDNLPSLQATVNLLKSTTIQKVRLFGAEPAVIKAFANTGVEIVIGFDNGDIP 89

Query: 84  AMGDYKR-AQQWVQRNVTRYNFDGGVVIKYVAVGNEPFLSSYNGSFLNVTFPALQNIQNA 143
            +      A Q+V+ NV  +     ++   + VGNE  L+S +   ++   PA+QN+QNA
Sbjct: 90  TLASNPNVASQFVKSNVMSFYPASNIIA--ITVGNE-VLTSGDQKLISQLLPAMQNVQNA 149

Query: 144 LNEAGLGDSIKATVPLNADVYNSPVNSPYPSAGRFRSDINGLMTDIVQFLSKNNAPFTVN 203
           LN A LG  +K +      V +    S  PS+G F   +   M  ++QF S N+APF ++
Sbjct: 150 LNAASLGGKVKVSTVHAMAVLS---QSYPPSSGVFNPGLGDTMKALLQFQSANDAPFMIS 209

Query: 204 IYPFLSLYGNDNFPFDYAFFDGASNP-VVDAGNGIQYTNVFDANFDTLVSSLKAVGHGDM 263
            YP+ + Y N   P   AF     N   VD+GNG +YTN+FDA  D + S+L A+G  D+
Sbjct: 210 PYPYFA-YKNQPTPDTLAFCLFQPNAGQVDSGNGHKYTNMFDAQVDAVHSALNAMGFKDI 269

Query: 264 PILVGEVGWPTDGDKN---ANVGNAYRFYNGLLPRLAASKGTPLRAG-YIEVYLFSFLDE 323
            I+V E GWP  GD N    ++ NA  +   L+  L +  GTPL  G  I+ YLFS  DE
Sbjct: 270 EIVVAETGWPHGGDSNEVGPSLDNAKAYVGNLINHLKSKVGTPLMPGKSIDTYLFSLYDE 329

Query: 324 NGKSIAPGNFERHWGIFGYDGQPKFGMDLSGQGQNKLLVGA-QDVQYLPRKWCMFNPNAK 383
           + K+ A  + E+++G+F  DG   + + L    QN                WC+  P   
Sbjct: 330 DKKTGA--SSEKYFGLFKPDGSTTYDVGLLKNTQNPTTPATPTPTPKAAGSWCVPKPGVS 389

Query: 384 DLSKLADNINYACTFS-DCTSLGYGSSCNNLD-ANGNASYAFNMYFQVQNQDEMACNFQG 443
           D  +L  NINYAC    DC  +  G +C   +    +A+Y  N+Y+Q   ++   C+F  
Sbjct: 390 D-DQLTGNINYACGQGIDCGPIQPGGACFEPNTVKAHAAYVMNLYYQSAGRNSWNCDFSQ 449

Query: 444 LATITSQNLSQGTCNF 451
            AT+T+ N S G CNF
Sbjct: 450 TATLTNTNPSYGACNF 455

BLAST of Moc05g14520 vs. ExPASy TrEMBL
Match: A0A6J1DNQ1 ((1->3)-beta-glucan endohydrolase OS=Momordica charantia OX=3673 GN=LOC111022903 PE=3 SV=1)

HSP 1 Score: 980.7 bits (2534), Expect = 2.2e-282
Identity = 480/481 (99.79%), Postives = 480/481 (99.79%), Query Frame = 0

Query: 1   MERRLGFLKWVLLVGVLGCCVEGLGVNWGTMANHRLPPKTVVQMFKDNGIKKVKLFDADQ 60
           MERRLGFLKWVLLVGVLGCCVEGLGVNWGTMANHRLPPKTVVQMFKDNGIKKVKLFDADQ
Sbjct: 1   MERRLGFLKWVLLVGVLGCCVEGLGVNWGTMANHRLPPKTVVQMFKDNGIKKVKLFDADQ 60

Query: 61  STMGALAGSGIEVMVAIPNDQLAAMGDYKRAQQWVQRNVTRYNFDGGVVIKYVAVGNEPF 120
           STMGALAGSGIEVMVAIPNDQLAAMGDYKRAQQWVQRNVTRYNFDGGVVIKYVAVGNEPF
Sbjct: 61  STMGALAGSGIEVMVAIPNDQLAAMGDYKRAQQWVQRNVTRYNFDGGVVIKYVAVGNEPF 120

Query: 121 LSSYNGSFLNVTFPALQNIQNALNEAGLGDSIKATVPLNADVYNSPVNSPYPSAGRFRSD 180
           LSSYNGSFLNVTFPALQNIQNALNEAGLGDSIKATVPLNADVYNSPVNSPYPSAGRFRSD
Sbjct: 121 LSSYNGSFLNVTFPALQNIQNALNEAGLGDSIKATVPLNADVYNSPVNSPYPSAGRFRSD 180

Query: 181 INGLMTDIVQFLSKNNAPFTVNIYPFLSLYGNDNFPFDYAFFDGASNPVVDAGNGIQYTN 240
           INGLMTDIVQFLSKNNAPFTVNIYPFLSLYGNDNFPFDYAFFDGASNPVVDAGNGIQYTN
Sbjct: 181 INGLMTDIVQFLSKNNAPFTVNIYPFLSLYGNDNFPFDYAFFDGASNPVVDAGNGIQYTN 240

Query: 241 VFDANFDTLVSSLKAVGHGDMPILVGEVGWPTDGDKNANVGNAYRFYNGLLPRLAASKGT 300
           VFDANFDTLVSSLKAVGHGDMPILVGEVGWPTDGDKNANVGNAYRFYNGLLPRLAASKGT
Sbjct: 241 VFDANFDTLVSSLKAVGHGDMPILVGEVGWPTDGDKNANVGNAYRFYNGLLPRLAASKGT 300

Query: 301 PLRAGYIEVYLFSFLDENGKSIAPGNFERHWGIFGYDGQPKFGMDLSGQGQNKLLVGAQD 360
           PLRAGYIEVYLFSFLDENGKSIAPGNFERHWGIFGYDGQPKFGMDLSGQGQNKLLVGAQD
Sbjct: 301 PLRAGYIEVYLFSFLDENGKSIAPGNFERHWGIFGYDGQPKFGMDLSGQGQNKLLVGAQD 360

Query: 361 VQYLPRKWCMFNPNAKDLSKLADNINYACTFSDCTSLGYGSSCNNLDANGNASYAFNMYF 420
           VQYLPRKWCMFNPNAKDLSKLADNINYACTFSDCTSLGYGSSCNNLDANGNASYAFNMYF
Sbjct: 361 VQYLPRKWCMFNPNAKDLSKLADNINYACTFSDCTSLGYGSSCNNLDANGNASYAFNMYF 420

Query: 421 QVQNQDEMACNFQGLATITSQNLSQGTCNFIIQLGSSSPPPLAASFVSLASMVMLLTALL 480
           QVQNQDEMACNFQGLATITSQNLSQGTCNFIIQLGSSSPPPLAASFVSLASMVMLLTAL 
Sbjct: 421 QVQNQDEMACNFQGLATITSQNLSQGTCNFIIQLGSSSPPPLAASFVSLASMVMLLTALX 480

Query: 481 L 482
           L
Sbjct: 481 L 481

BLAST of Moc05g14520 vs. ExPASy TrEMBL
Match: A0A0A0LFN2 ((1->3)-beta-glucan endohydrolase OS=Cucumis sativus OX=3659 GN=Csa_3G843830 PE=3 SV=1)

HSP 1 Score: 903.7 bits (2334), Expect = 3.4e-259
Identity = 442/477 (92.66%), Postives = 459/477 (96.23%), Query Frame = 0

Query: 5   LGFLKWVLLVGVLGCCVEGLGVNWGTMANHRLPPKTVVQMFKDNGIKKVKLFDADQSTMG 64
           LGFLKWVLLVGVLG CVEGLGVNWGTMANHRLPPKTVVQMFKDNGI+KVKLFDADQS+MG
Sbjct: 4   LGFLKWVLLVGVLGSCVEGLGVNWGTMANHRLPPKTVVQMFKDNGIQKVKLFDADQSSMG 63

Query: 65  ALAGSGIEVMVAIPNDQLAAMGDYKRAQQWVQRNVTRYNFDGGVVIKYVAVGNEPFLSSY 124
           ALAG+GIEVMVAIPNDQL+AMGDY RA+QWVQRNVTRY FDGGV IKYVAVGNEPFLSSY
Sbjct: 64  ALAGTGIEVMVAIPNDQLSAMGDYNRAKQWVQRNVTRYTFDGGVTIKYVAVGNEPFLSSY 123

Query: 125 NGSFLNVTFPALQNIQNALNEAGLGDSIKATVPLNADVYNSPVNSPYPSAGRFRSDINGL 184
           NGSFLNVTFPAL NIQNALNEAG GDSIKATVPLNADVYNSPVNSPYPSAGRFR+DIN L
Sbjct: 124 NGSFLNVTFPALLNIQNALNEAGHGDSIKATVPLNADVYNSPVNSPYPSAGRFRNDINQL 183

Query: 185 MTDIVQFLSKNNAPFTVNIYPFLSLYGNDNFPFDYAFFDGASNPVVDAGNGIQYTNVFDA 244
           MTDIVQFL+KN APFTVNIYPFLSLYGNDNFPFDYAFFDGASNPVVD G GIQYTNVFDA
Sbjct: 184 MTDIVQFLNKNKAPFTVNIYPFLSLYGNDNFPFDYAFFDGASNPVVDIGTGIQYTNVFDA 243

Query: 245 NFDTLVSSLKAVGHGDMPILVGEVGWPTDGDKNANVGNAYRFYNGLLPRLAASKGTPLRA 304
           NFDTLVSSLKAVG GDMPILVGEVGWPTDGDKN NVGNAYRFYNGLLPRLAA+KGTPLR 
Sbjct: 244 NFDTLVSSLKAVGLGDMPILVGEVGWPTDGDKNGNVGNAYRFYNGLLPRLAANKGTPLRP 303

Query: 305 GYIEVYLFSFLDENGKSIAPGNFERHWGIFGYDGQPKFGMDLSGQGQNKLLVGAQDVQYL 364
           G+IEVYLFSFLDENGKSIAPGNFERHWGIFGYDGQPKFGMDLSGQGQNKLLVGAQ+VQYL
Sbjct: 304 GFIEVYLFSFLDENGKSIAPGNFERHWGIFGYDGQPKFGMDLSGQGQNKLLVGAQNVQYL 363

Query: 365 PRKWCMFNPNAKDLSKLADNINYACTFSDCTSLGYGSSCNNLDANGNASYAFNMYFQVQN 424
           P+KWCMFNPNAKDLSKLADNI+YACTFSDCTSLGYGSSCNNLDANGNASYAFNMYFQVQN
Sbjct: 364 PQKWCMFNPNAKDLSKLADNIDYACTFSDCTSLGYGSSCNNLDANGNASYAFNMYFQVQN 423

Query: 425 QDEMACNFQGLATITSQNLSQGTCNFIIQLGSSSPPPLAASFVSLASMVMLLTALLL 482
           QDE+ACNFQGLAT+TSQNLSQGTCNFIIQL +SSPP  AASFVSLASM++L  ALLL
Sbjct: 424 QDELACNFQGLATVTSQNLSQGTCNFIIQL-ASSPPSHAASFVSLASMILLFIALLL 479

BLAST of Moc05g14520 vs. ExPASy TrEMBL
Match: A0A5D3E281 ((1->3)-beta-glucan endohydrolase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold734G00130 PE=3 SV=1)

HSP 1 Score: 901.0 bits (2327), Expect = 2.2e-258
Identity = 443/478 (92.68%), Postives = 459/478 (96.03%), Query Frame = 0

Query: 4   RLGFLKWVLLVGVLGCCVEGLGVNWGTMANHRLPPKTVVQMFKDNGIKKVKLFDADQSTM 63
           RLGFLKWVLLVGVLG CVEGLGVNWGTMANHRLPPKTVVQM KDNGI+KVKLFDADQS+M
Sbjct: 3   RLGFLKWVLLVGVLGSCVEGLGVNWGTMANHRLPPKTVVQMLKDNGIQKVKLFDADQSSM 62

Query: 64  GALAGSGIEVMVAIPNDQLAAMGDYKRAQQWVQRNVTRYNFDGGVVIKYVAVGNEPFLSS 123
           GALAG+GIEVMVAIPNDQL+AMGDY RA+QWVQRNVTRY FDGGV IKYVAVGNEPFLSS
Sbjct: 63  GALAGTGIEVMVAIPNDQLSAMGDYNRAKQWVQRNVTRYIFDGGVTIKYVAVGNEPFLSS 122

Query: 124 YNGSFLNVTFPALQNIQNALNEAGLGDSIKATVPLNADVYNSPVNSPYPSAGRFRSDING 183
           YNGSFLNVTFPALQNIQNALNEAG GDSIKATVPLNADVYNSPVNSPYPSAGRFRSDIN 
Sbjct: 123 YNGSFLNVTFPALQNIQNALNEAGHGDSIKATVPLNADVYNSPVNSPYPSAGRFRSDINQ 182

Query: 184 LMTDIVQFLSKNNAPFTVNIYPFLSLYGNDNFPFDYAFFDGASNPVVDAGNGIQYTNVFD 243
           LM DIVQFL+KN APFTVNIYPFLSLYGNDNFPFDYAFFDGASNPVVD G GIQYTNVFD
Sbjct: 183 LMNDIVQFLNKNKAPFTVNIYPFLSLYGNDNFPFDYAFFDGASNPVVDIGTGIQYTNVFD 242

Query: 244 ANFDTLVSSLKAVGHGDMPILVGEVGWPTDGDKNANVGNAYRFYNGLLPRLAASKGTPLR 303
           ANFDTLVSSLKAVG GDMPILVGEVGWPTDGDKNAN GNAYRFYNGLLPRLAA+KGTPLR
Sbjct: 243 ANFDTLVSSLKAVGLGDMPILVGEVGWPTDGDKNANDGNAYRFYNGLLPRLAANKGTPLR 302

Query: 304 AGYIEVYLFSFLDENGKSIAPGNFERHWGIFGYDGQPKFGMDLSGQGQNKLLVGAQDVQY 363
            G+IEVYLFSFLDENGKSIAPGNFERHWGIFGYDGQPKFGMDLSGQGQNKLLVGAQ+V+Y
Sbjct: 303 PGFIEVYLFSFLDENGKSIAPGNFERHWGIFGYDGQPKFGMDLSGQGQNKLLVGAQNVEY 362

Query: 364 LPRKWCMFNPNAKDLSKLADNINYACTFSDCTSLGYGSSCNNLDANGNASYAFNMYFQVQ 423
           LP+KWCMFNPNAKDLSKLADNI+YACTFSDCTSLGYGSSCNNLDANGNASYAFNMYFQVQ
Sbjct: 363 LPQKWCMFNPNAKDLSKLADNIDYACTFSDCTSLGYGSSCNNLDANGNASYAFNMYFQVQ 422

Query: 424 NQDEMACNFQGLATITSQNLSQGTCNFIIQLGSSSPPPLAASFVSLASMVMLLTALLL 482
           NQDE+ACNFQGLATITS+NLSQGTCNFIIQL +SSPP  AASFVSLASMV+L  ALLL
Sbjct: 423 NQDELACNFQGLATITSRNLSQGTCNFIIQL-ASSPPSHAASFVSLASMVLLFIALLL 479

BLAST of Moc05g14520 vs. ExPASy TrEMBL
Match: A0A1S3CNJ0 ((1->3)-beta-glucan endohydrolase OS=Cucumis melo OX=3656 GN=LOC103502975 PE=3 SV=1)

HSP 1 Score: 901.0 bits (2327), Expect = 2.2e-258
Identity = 443/478 (92.68%), Postives = 459/478 (96.03%), Query Frame = 0

Query: 4   RLGFLKWVLLVGVLGCCVEGLGVNWGTMANHRLPPKTVVQMFKDNGIKKVKLFDADQSTM 63
           RLGFLKWVLLVGVLG CVEGLGVNWGTMANHRLPPKTVVQM KDNGI+KVKLFDADQS+M
Sbjct: 3   RLGFLKWVLLVGVLGSCVEGLGVNWGTMANHRLPPKTVVQMLKDNGIQKVKLFDADQSSM 62

Query: 64  GALAGSGIEVMVAIPNDQLAAMGDYKRAQQWVQRNVTRYNFDGGVVIKYVAVGNEPFLSS 123
           GALAG+GIEVMVAIPNDQL+AMGDY RA+QWVQRNVTRY FDGGV IKYVAVGNEPFLSS
Sbjct: 63  GALAGTGIEVMVAIPNDQLSAMGDYNRAKQWVQRNVTRYIFDGGVTIKYVAVGNEPFLSS 122

Query: 124 YNGSFLNVTFPALQNIQNALNEAGLGDSIKATVPLNADVYNSPVNSPYPSAGRFRSDING 183
           YNGSFLNVTFPALQNIQNALNEAG GDSIKATVPLNADVYNSPVNSPYPSAGRFRSDIN 
Sbjct: 123 YNGSFLNVTFPALQNIQNALNEAGHGDSIKATVPLNADVYNSPVNSPYPSAGRFRSDINQ 182

Query: 184 LMTDIVQFLSKNNAPFTVNIYPFLSLYGNDNFPFDYAFFDGASNPVVDAGNGIQYTNVFD 243
           LM DIVQFL+KN APFTVNIYPFLSLYGNDNFPFDYAFFDGASNPVVD G GIQYTNVFD
Sbjct: 183 LMNDIVQFLNKNKAPFTVNIYPFLSLYGNDNFPFDYAFFDGASNPVVDIGTGIQYTNVFD 242

Query: 244 ANFDTLVSSLKAVGHGDMPILVGEVGWPTDGDKNANVGNAYRFYNGLLPRLAASKGTPLR 303
           ANFDTLVSSLKAVG GDMPILVGEVGWPTDGDKNAN GNAYRFYNGLLPRLAA+KGTPLR
Sbjct: 243 ANFDTLVSSLKAVGLGDMPILVGEVGWPTDGDKNANDGNAYRFYNGLLPRLAANKGTPLR 302

Query: 304 AGYIEVYLFSFLDENGKSIAPGNFERHWGIFGYDGQPKFGMDLSGQGQNKLLVGAQDVQY 363
            G+IEVYLFSFLDENGKSIAPGNFERHWGIFGYDGQPKFGMDLSGQGQNKLLVGAQ+V+Y
Sbjct: 303 PGFIEVYLFSFLDENGKSIAPGNFERHWGIFGYDGQPKFGMDLSGQGQNKLLVGAQNVEY 362

Query: 364 LPRKWCMFNPNAKDLSKLADNINYACTFSDCTSLGYGSSCNNLDANGNASYAFNMYFQVQ 423
           LP+KWCMFNPNAKDLSKLADNI+YACTFSDCTSLGYGSSCNNLDANGNASYAFNMYFQVQ
Sbjct: 363 LPQKWCMFNPNAKDLSKLADNIDYACTFSDCTSLGYGSSCNNLDANGNASYAFNMYFQVQ 422

Query: 424 NQDEMACNFQGLATITSQNLSQGTCNFIIQLGSSSPPPLAASFVSLASMVMLLTALLL 482
           NQDE+ACNFQGLATITS+NLSQGTCNFIIQL +SSPP  AASFVSLASMV+L  ALLL
Sbjct: 423 NQDELACNFQGLATITSRNLSQGTCNFIIQL-ASSPPSHAASFVSLASMVLLFIALLL 479

BLAST of Moc05g14520 vs. ExPASy TrEMBL
Match: A0A6J1I9Q3 ((1->3)-beta-glucan endohydrolase OS=Cucurbita maxima OX=3661 GN=LOC111472848 PE=3 SV=1)

HSP 1 Score: 897.5 bits (2318), Expect = 2.4e-257
Identity = 435/478 (91.00%), Postives = 454/478 (94.98%), Query Frame = 0

Query: 4   RLGFLKWVLLVGVLGCCVEGLGVNWGTMANHRLPPKTVVQMFKDNGIKKVKLFDADQSTM 63
           RL  L WVLLVGVLGCC+EGLGVNWGTMANH+LPPKT+VQMFKDNGI+KVKLFDADQS+M
Sbjct: 3   RLWILNWVLLVGVLGCCIEGLGVNWGTMANHKLPPKTIVQMFKDNGIQKVKLFDADQSSM 62

Query: 64  GALAGSGIEVMVAIPNDQLAAMGDYKRAQQWVQRNVTRYNFDGGVVIKYVAVGNEPFLSS 123
           GALAG+GIEVMVAIPNDQL AMGDY RA+QWVQRNV+RY FDGGV IKYVAVGNEPFLSS
Sbjct: 63  GALAGTGIEVMVAIPNDQLVAMGDYNRAKQWVQRNVSRYTFDGGVTIKYVAVGNEPFLSS 122

Query: 124 YNGSFLNVTFPALQNIQNALNEAGLGDSIKATVPLNADVYNSPVNSPYPSAGRFRSDING 183
           YNGSFLNVTFPALQN+QNALNEAG GD+IKATVPLNADVYNSPVN+PYPSAGRFRSDING
Sbjct: 123 YNGSFLNVTFPALQNVQNALNEAGYGDTIKATVPLNADVYNSPVNTPYPSAGRFRSDING 182

Query: 184 LMTDIVQFLSKNNAPFTVNIYPFLSLYGNDNFPFDYAFFDGASNPVVDAGNGIQYTNVFD 243
           LMT IVQFLSKNNAPFTVNIYPFLSLYGNDNFPFDYAFFDGASNPVVD   GIQYTNVFD
Sbjct: 183 LMTQIVQFLSKNNAPFTVNIYPFLSLYGNDNFPFDYAFFDGASNPVVDVNTGIQYTNVFD 242

Query: 244 ANFDTLVSSLKAVGHGDMPILVGEVGWPTDGDKNANVGNAYRFYNGLLPRLAASKGTPLR 303
           ANFDTLVSSLKAVG GDMPILVGEVGWPTDGDKNAN GNAYRFYNGLLPRLAA+KGTPLR
Sbjct: 243 ANFDTLVSSLKAVGLGDMPILVGEVGWPTDGDKNANAGNAYRFYNGLLPRLAANKGTPLR 302

Query: 304 AGYIEVYLFSFLDENGKSIAPGNFERHWGIFGYDGQPKFGMDLSGQGQNKLLVGAQDVQY 363
            G+IEVYLFSFLDENGKSIAPGNFERHWGIFGYDGQPKFGMDLSGQGQNKLLVGAQDVQY
Sbjct: 303 PGFIEVYLFSFLDENGKSIAPGNFERHWGIFGYDGQPKFGMDLSGQGQNKLLVGAQDVQY 362

Query: 364 LPRKWCMFNPNAKDLSKLADNINYACTFSDCTSLGYGSSCNNLDANGNASYAFNMYFQVQ 423
           LP+KWCMFNPNAKDLSKLADNI+YACTFSDCTSLGYGSSCNNLDANGNASYAFNMYFQVQ
Sbjct: 363 LPQKWCMFNPNAKDLSKLADNIDYACTFSDCTSLGYGSSCNNLDANGNASYAFNMYFQVQ 422

Query: 424 NQDEMACNFQGLATITSQNLSQGTCNFIIQLGSSSPPPLAASFVSLASMVMLLTALLL 482
           NQDE+ACNFQGLATI+SQNLSQGTC FIIQL SSS P   ASFVSL SM +L TA+LL
Sbjct: 423 NQDELACNFQGLATISSQNLSQGTCKFIIQLASSS-PSFDASFVSLVSMALLFTAMLL 479

BLAST of Moc05g14520 vs. TAIR 10
Match: AT1G64760.1 (O-Glycosyl hydrolases family 17 protein )

HSP 1 Score: 730.7 bits (1885), Expect = 7.5e-211
Identity = 352/465 (75.70%), Postives = 399/465 (85.81%), Query Frame = 0

Query: 10  WVLLVGVLGCCVEGLGVNWGTMANHRLPPKTVVQMFKDNGIKKVKLFDADQSTMGALAGS 69
           +V+++G LG  V GLGVNWGTMA H+LPPKTVVQM KDN I KVKLFDAD++TMGALAGS
Sbjct: 11  FVIVIGHLGILVNGLGVNWGTMATHKLPPKTVVQMLKDNNINKVKLFDADETTMGALAGS 70

Query: 70  GIEVMVAIPNDQLAAMGDYKRAQQWVQRNVTRYNFDGGVVIKYVAVGNEPFLSSYNGSFL 129
           G+EVMVAIPNDQL  M  Y RA+ WV++NVTRYNFDGGV I +VAVGNEPFL SYNGSF+
Sbjct: 71  GLEVMVAIPNDQLKVMTSYDRAKDWVRKNVTRYNFDGGVNITFVAVGNEPFLKSYNGSFI 130

Query: 130 NVTFPALQNIQNALNEAGLGDSIKATVPLNADVYNSPVNSPYPSAGRFRSDINGLMTDIV 189
           N+TFPAL NIQNALNEAGLG+S+KATVPLNADVY+SP ++P PSAGRFR DI G MT IV
Sbjct: 131 NLTFPALANIQNALNEAGLGNSVKATVPLNADVYDSPASNPVPSAGRFRPDIIGQMTQIV 190

Query: 190 QFLSKNNAPFTVNIYPFLSLYGNDNFPFDYAFFDGASNPVVDAGNGIQYTNVFDANFDTL 249
            FL KNNAP T+NIYPFLSLYGND+FP +YAFFDGA  P+ D  NGI YTNVFDANFDTL
Sbjct: 191 DFLGKNNAPITINIYPFLSLYGNDDFPLNYAFFDGA-EPIND--NGIDYTNVFDANFDTL 250

Query: 250 VSSLKAVGHGDMPILVGEVGWPTDGDKNANVGNAYRFYNGLLPRLAASKGTPLRAGYIEV 309
           VSSLKAVGHGDMPI+VGEVGWPT+GDK+AN G+AYRFYNGLLPRL  +KGTPLR  YIEV
Sbjct: 251 VSSLKAVGHGDMPIIVGEVGWPTEGDKHANAGSAYRFYNGLLPRLGTNKGTPLRPTYIEV 310

Query: 310 YLFSFLDENGKSIAPGNFERHWGIFGYDGQPKFGMDLSGQGQNKLLVGAQDVQYLPRKWC 369
           YLF  LDE+ KSIAPG FERHWGIF +DGQPKF +DLSGQGQ+K L+GAQ+V YLP KWC
Sbjct: 311 YLFGLLDEDAKSIAPGPFERHWGIFKFDGQPKFPIDLSGQGQSKFLIGAQNVPYLPNKWC 370

Query: 370 MFNPNAKDLSKLADNINYACTFSDCTSLGYGSSCNNLDANGNASYAFNMYFQVQNQDEMA 429
            FNP AKDL+KLA NI+YACTFSDCT+LGYGSSCN LDANGNASYAFNM+FQV+NQDE A
Sbjct: 371 TFNPEAKDLTKLAANIDYACTFSDCTALGYGSSCNTLDANGNASYAFNMFFQVKNQDESA 430

Query: 430 CNFQGLATITSQNLSQGTCNFIIQLGSSSPPPLAASFVSLASMVM 475
           C FQGLATIT+QN+SQG CNF IQ+ +SS    + S  SL  +++
Sbjct: 431 CYFQGLATITTQNISQGQCNFPIQIVASSASSFSCSSYSLVVLIV 472

BLAST of Moc05g14520 vs. TAIR 10
Match: AT1G64760.2 (O-Glycosyl hydrolases family 17 protein )

HSP 1 Score: 730.7 bits (1885), Expect = 7.5e-211
Identity = 352/465 (75.70%), Postives = 399/465 (85.81%), Query Frame = 0

Query: 10  WVLLVGVLGCCVEGLGVNWGTMANHRLPPKTVVQMFKDNGIKKVKLFDADQSTMGALAGS 69
           +V+++G LG  V GLGVNWGTMA H+LPPKTVVQM KDN I KVKLFDAD++TMGALAGS
Sbjct: 11  FVIVIGHLGILVNGLGVNWGTMATHKLPPKTVVQMLKDNNINKVKLFDADETTMGALAGS 70

Query: 70  GIEVMVAIPNDQLAAMGDYKRAQQWVQRNVTRYNFDGGVVIKYVAVGNEPFLSSYNGSFL 129
           G+EVMVAIPNDQL  M  Y RA+ WV++NVTRYNFDGGV I +VAVGNEPFL SYNGSF+
Sbjct: 71  GLEVMVAIPNDQLKVMTSYDRAKDWVRKNVTRYNFDGGVNITFVAVGNEPFLKSYNGSFI 130

Query: 130 NVTFPALQNIQNALNEAGLGDSIKATVPLNADVYNSPVNSPYPSAGRFRSDINGLMTDIV 189
           N+TFPAL NIQNALNEAGLG+S+KATVPLNADVY+SP ++P PSAGRFR DI G MT IV
Sbjct: 131 NLTFPALANIQNALNEAGLGNSVKATVPLNADVYDSPASNPVPSAGRFRPDIIGQMTQIV 190

Query: 190 QFLSKNNAPFTVNIYPFLSLYGNDNFPFDYAFFDGASNPVVDAGNGIQYTNVFDANFDTL 249
            FL KNNAP T+NIYPFLSLYGND+FP +YAFFDGA  P+ D  NGI YTNVFDANFDTL
Sbjct: 191 DFLGKNNAPITINIYPFLSLYGNDDFPLNYAFFDGA-EPIND--NGIDYTNVFDANFDTL 250

Query: 250 VSSLKAVGHGDMPILVGEVGWPTDGDKNANVGNAYRFYNGLLPRLAASKGTPLRAGYIEV 309
           VSSLKAVGHGDMPI+VGEVGWPT+GDK+AN G+AYRFYNGLLPRL  +KGTPLR  YIEV
Sbjct: 251 VSSLKAVGHGDMPIIVGEVGWPTEGDKHANAGSAYRFYNGLLPRLGTNKGTPLRPTYIEV 310

Query: 310 YLFSFLDENGKSIAPGNFERHWGIFGYDGQPKFGMDLSGQGQNKLLVGAQDVQYLPRKWC 369
           YLF  LDE+ KSIAPG FERHWGIF +DGQPKF +DLSGQGQ+K L+GAQ+V YLP KWC
Sbjct: 311 YLFGLLDEDAKSIAPGPFERHWGIFKFDGQPKFPIDLSGQGQSKFLIGAQNVPYLPNKWC 370

Query: 370 MFNPNAKDLSKLADNINYACTFSDCTSLGYGSSCNNLDANGNASYAFNMYFQVQNQDEMA 429
            FNP AKDL+KLA NI+YACTFSDCT+LGYGSSCN LDANGNASYAFNM+FQV+NQDE A
Sbjct: 371 TFNPEAKDLTKLAANIDYACTFSDCTALGYGSSCNTLDANGNASYAFNMFFQVKNQDESA 430

Query: 430 CNFQGLATITSQNLSQGTCNFIIQLGSSSPPPLAASFVSLASMVM 475
           C FQGLATIT+QN+SQG CNF IQ+ +SS    + S  SL  +++
Sbjct: 431 CYFQGLATITTQNISQGQCNFPIQIVASSASSFSCSSYSLVVLIV 472

BLAST of Moc05g14520 vs. TAIR 10
Match: AT2G19440.1 (O-Glycosyl hydrolases family 17 protein )

HSP 1 Score: 713.4 bits (1840), Expect = 1.2e-205
Identity = 348/473 (73.57%), Postives = 400/473 (84.57%), Query Frame = 0

Query: 5   LGFLKWVLLVGVLG-CCVEGLGVNWGTMANHRLPPKTVVQMFKDNGIKKVKLFDADQSTM 64
           +  L +V+  G++G   V+GLGVNWGTMA H+LPPK VVQM KDN I KVKLFDAD++TM
Sbjct: 1   MNLLAFVVGFGIMGIVMVDGLGVNWGTMATHKLPPKKVVQMLKDNNINKVKLFDADETTM 60

Query: 65  GALAGSGIEVMVAIPNDQLAAMGDYKRAQQWVQRNVTRYNFDGGVVIKYVAVGNEPFLSS 124
            AL+GSG+EVMVAIPNDQL  MG Y RA+ WV +NVTRYNF+GGV I +VAVGNEPFL S
Sbjct: 61  SALSGSGLEVMVAIPNDQLKVMGSYDRAKDWVHKNVTRYNFNGGVNITFVAVGNEPFLKS 120

Query: 125 YNGSFLNVTFPALQNIQNALNEAGLGDSIKATVPLNADVYNSPVNSPYPSAGRFRSDING 184
           YNGSF+N+TFPALQNIQNALNEAGLG S+KATVPLNADVY+SP ++P PSAGRFR DI G
Sbjct: 121 YNGSFINLTFPALQNIQNALNEAGLGSSVKATVPLNADVYDSPSSNPVPSAGRFRPDIIG 180

Query: 185 LMTDIVQFLSKNNAPFTVNIYPFLSLYGNDNFPFDYAFFDGASNPVVDAGNGIQYTNVFD 244
            MT IV FL  N+AP T+NIYPFLSLYGND+FP +YAFFDGA  PV D  NGI YTNVFD
Sbjct: 181 QMTQIVDFLGNNSAPITINIYPFLSLYGNDDFPLNYAFFDGA-KPVDD--NGIAYTNVFD 240

Query: 245 ANFDTLVSSLKAVGHGDMPILVGEVGWPTDGDKNANVGNAYRFYNGLLPRLAASKGTPLR 304
           ANFDTLVS+LKAVGHGDMPI+VGEVGWPT+GDK+AN G+AYRFYNGLLPRL  ++GTPLR
Sbjct: 241 ANFDTLVSALKAVGHGDMPIIVGEVGWPTEGDKHANSGSAYRFYNGLLPRLGENRGTPLR 300

Query: 305 AGYIEVYLFSFLDENGKSIAPGNFERHWGIFGYDGQPKFGMDLSGQGQNKLLVGAQDVQY 364
             YIEVYLF  LDE+ KSIAPG FERHWGIF +DGQPKF +DLSGQGQNKLL+GA++V Y
Sbjct: 301 PTYIEVYLFGLLDEDAKSIAPGEFERHWGIFKFDGQPKFPIDLSGQGQNKLLIGAENVTY 360

Query: 365 LPRKWCMFNPNAKDLSKLADNINYACTFSDCTSLGYGSSCNNLDANGNASYAFNMYFQVQ 424
            P+KWCMFN  AKDL+KLA NI+YACTFSDCT+LGYGSSCN LDANGNASYAFNMYFQV+
Sbjct: 361 QPKKWCMFNTEAKDLTKLAANIDYACTFSDCTALGYGSSCNTLDANGNASYAFNMYFQVK 420

Query: 425 NQDEMACNFQGLATITSQNLSQGTCNFIIQLGSSSPPPLAASFVSLASMVMLL 477
           NQDE AC FQGLATIT++N+SQG CNF IQ+ +S+    ++S V L + V  L
Sbjct: 421 NQDEDACIFQGLATITTKNISQGQCNFPIQIVASTASSFSSSLVLLIAGVWFL 470

BLAST of Moc05g14520 vs. TAIR 10
Match: AT3G04010.1 (O-Glycosyl hydrolases family 17 protein )

HSP 1 Score: 673.7 bits (1737), Expect = 1.1e-193
Identity = 325/461 (70.50%), Postives = 388/461 (84.16%), Query Frame = 0

Query: 21  VEGLGVNWGTMANHRLPPKTVVQMFKDNGIKKVKLFDADQSTMGALAGSGIEVMVAIPND 80
           V GLGVNWGTMA+H+LPPKTVV+M KDN I+KVKLFDAD +TMGALAGSG+EVMVAIPND
Sbjct: 30  VSGLGVNWGTMASHQLPPKTVVEMLKDNNIQKVKLFDADTNTMGALAGSGVEVMVAIPND 89

Query: 81  QLAAMGDYKRAQQWVQRNVTRYNFDGGVVIKYVAVGNEPFLSSYNGSFLNVTFPALQNIQ 140
            L AMG+Y+RA+ WVQRNV+R+NF+ GV IKYVAVGNEPFL++YNGSF+N+T+PAL NIQ
Sbjct: 90  LLLAMGNYQRAKDWVQRNVSRFNFNNGVKIKYVAVGNEPFLTAYNGSFINLTYPALFNIQ 149

Query: 141 NALNEAGLGDSIKATVPLNADVYNSPVNSPYPSAGRFRSDINGLMTDIVQFLSKNNAPFT 200
            ALNEAG+GD  KATVPLNADVYNSP ++  PSAGRFRSDI   MT IV FL++N APFT
Sbjct: 150 TALNEAGVGDFTKATVPLNADVYNSPPDNQVPSAGRFRSDIIQEMTQIVNFLAQNKAPFT 209

Query: 201 VNIYPFLSLYGNDNFPFDYAFFDGASNPVVDAGNGIQYTNVFDANFDTLVSSLKAVGHGD 260
           VNIYPFLSLY + +FPF+YAFFDG  N V D  NG+ YTNVFDA+FDTL++SL A+ HG+
Sbjct: 210 VNIYPFLSLYLSSDFPFEYAFFDG-QNTVND--NGVIYTNVFDASFDTLLASLNALNHGN 269

Query: 261 MPILVGEVGWPTDGDKNANVGNAYRFYNGLLPRLAASKGTPLRAGYIEVYLFSFLDENGK 320
           M ++VGEVGWPTDGDKNANV NA RFY+GLLPRLA + GTP+R GYIEVYLF F+DE+ K
Sbjct: 270 MEVIVGEVGWPTDGDKNANVPNAERFYSGLLPRLANNVGTPMRKGYIEVYLFGFIDEDAK 329

Query: 321 SIAPGNFERHWGIFGYDGQPKFGMDLSGQGQNKLLVGAQDVQYLPRKWCMFNPNAK-DLS 380
           S+APGNFERHWGIF +DGQPKF +D  GQGQ K L GAQ+VQY   +WCMFNPN + ++S
Sbjct: 330 SVAPGNFERHWGIFKFDGQPKFPVDFRGQGQKKFLTGAQNVQYFLNQWCMFNPNGRGNMS 389

Query: 381 KLADNINYACTFSDCTSLGYGSSCNNLDANGNASYAFNMYFQVQNQDEMACNFQGLATIT 440
           +L DNINYAC+ SDCT+LGYGSSC NLDANGNASYAFNMYFQVQNQ+  AC+F+GLATIT
Sbjct: 390 RLGDNINYACSHSDCTALGYGSSCGNLDANGNASYAFNMYFQVQNQEAQACDFEGLATIT 449

Query: 441 SQNLSQGTCNFIIQLGSSSPPPLAASFVSLASMVMLLTALL 481
           +QN+SQG CNF IQ+G  S      S+ S+  + ++++ L+
Sbjct: 450 TQNISQGQCNFPIQIGEPSSGHYDYSYGSMVRLCLVMSGLV 487

BLAST of Moc05g14520 vs. TAIR 10
Match: AT5G18220.1 (O-Glycosyl hydrolases family 17 protein )

HSP 1 Score: 662.1 bits (1707), Expect = 3.3e-190
Identity = 333/482 (69.09%), Postives = 388/482 (80.50%), Query Frame = 0

Query: 6   GFLKWVLLVGVLGCCVEGLGVNWGTMANHRLPPKTVVQMFKDNGIKKVKLFDADQSTMGA 65
           G    + +V +L   V  LGVNWGTM+ H+LPPKTVVQM KDN +KKVKLFDAD +TM A
Sbjct: 12  GIFLCITIVSLLSGDVSALGVNWGTMSTHQLPPKTVVQMLKDNNVKKVKLFDADTNTMVA 71

Query: 66  LAGSGIEVMVAIPNDQLAAMGDYKRAQQWVQRNVTRYNFDGGVVIKYVAVGNEPFLSSYN 125
           LAGSGIEVMVAIPNDQL AMG Y RA+ WV+RN+TR+N D  V IKYVAVGNEPFL++YN
Sbjct: 72  LAGSGIEVMVAIPNDQLKAMGSYNRAKDWVRRNITRFNDD--VKIKYVAVGNEPFLTAYN 131

Query: 126 GSFLNVTFPALQNIQNALNEAGLGDSIKATVPLNADVYNSPVNSPYPSAGRFRSDINGLM 185
           GSF+N+T+PAL NIQ ALNEAG+GD IKATVPLNADVYNSP+ +P PSAG FR DI   M
Sbjct: 132 GSFINLTYPALFNIQKALNEAGVGDFIKATVPLNADVYNSPLENPVPSAGSFRQDIFEEM 191

Query: 186 TDIVQFLSKNNAPFTVNIYPFLSLYGNDNFPFDYAFFDGASNPVVDAGNGIQYTNVFDAN 245
             IV FL+ N APFTVNIYPFLSLY + +FPFDYAFF+G  N V D  NG+ YTNVFDAN
Sbjct: 192 KLIVNFLAHNKAPFTVNIYPFLSLYLSSDFPFDYAFFNG-QNTVSD--NGVIYTNVFDAN 251

Query: 246 FDTLVSSLKAVGHGDMPILVGEVGWPTDGDKNANVGNAYRFYNGLLPRLAASKGTPLRAG 305
           FDTL++SLKA+GHGDM ++VGEVGWPTDGDKNAN+ NA RFY GLLP+LAA++GTP+R G
Sbjct: 252 FDTLLASLKALGHGDMTVIVGEVGWPTDGDKNANIPNAERFYTGLLPKLAANRGTPMRPG 311

Query: 306 YIEVYLFSFLDENGKSIAPGNFERHWGIFGYDGQPKFGMDLSGQGQNKLLVGAQDVQYLP 365
           YIEVYLF F+DE+ KSIAPGNFERHWGIF YDGQPKF  DLSG GQ K+L GAQ+VQYL 
Sbjct: 312 YIEVYLFGFIDEDAKSIAPGNFERHWGIFKYDGQPKFPADLSGAGQKKILTGAQNVQYLR 371

Query: 366 RKWCMFNPNA----KDLSKLADNINYACTFSDCTSLGYGSSCNNLDANGNASYAFNMYFQ 425
            +WCMFNPNA     + ++L DN+NYACTFSDCT+LGYGSSC NLD  GNASYAFNMYFQ
Sbjct: 372 NQWCMFNPNALTFSNNTNQLGDNVNYACTFSDCTALGYGSSCGNLDEVGNASYAFNMYFQ 431

Query: 426 VQNQDEMACNFQGLATITSQNLSQGTCNFIIQLG--SSSPPPLAASFV--SLASMVMLLT 480
           VQNQ   AC+F+GLA IT++N+S+  CNF IQ+G  +S        F+  SL SM++L T
Sbjct: 432 VQNQKAEACDFEGLAIITTRNISREQCNFPIQIGDPTSGHSDHNHRFISFSLVSMLLLFT 488

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022155900.14.5e-28299.79glucan endo-1,3-beta-glucosidase 8-like [Momordica charantia][more]
XP_038903901.12.8e-26093.10glucan endo-1,3-beta-glucosidase 8-like [Benincasa hispida][more]
XP_004141142.17.0e-25992.66glucan endo-1,3-beta-glucosidase 8 [Cucumis sativus] >KGN59764.1 hypothetical pr... [more]
XP_008465328.14.5e-25892.68PREDICTED: glucan endo-1,3-beta-glucosidase 8-like [Cucumis melo] >KAA0051277.1 ... [more]
KAG6581686.15.9e-25891.84Glucan endo-1,3-beta-glucosidase 8, partial [Cucurbita argyrosperma subsp. soror... [more]
Match NameE-valueIdentityDescription
Q6NKW91.1e-20975.70Glucan endo-1,3-beta-glucosidase 8 OS=Arabidopsis thaliana OX=3702 GN=At1g64760 ... [more]
Q9M0885.9e-13652.61Glucan endo-1,3-beta-glucosidase 5 OS=Arabidopsis thaliana OX=3702 GN=At4g31140 ... [more]
Q93Z081.9e-13452.20Glucan endo-1,3-beta-glucosidase 6 OS=Arabidopsis thaliana OX=3702 GN=At5g58090 ... [more]
Q9FGH41.9e-9742.29Glucan endo-1,3-beta-glucosidase 9 OS=Arabidopsis thaliana OX=3702 GN=At5g58480 ... [more]
Q94G861.9e-5733.72Glucan endo-1,3-beta-D-glucosidase OS=Olea europaea OX=4146 GN=OLE9 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1DNQ12.2e-28299.79(1->3)-beta-glucan endohydrolase OS=Momordica charantia OX=3673 GN=LOC111022903 ... [more]
A0A0A0LFN23.4e-25992.66(1->3)-beta-glucan endohydrolase OS=Cucumis sativus OX=3659 GN=Csa_3G843830 PE=3... [more]
A0A5D3E2812.2e-25892.68(1->3)-beta-glucan endohydrolase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676... [more]
A0A1S3CNJ02.2e-25892.68(1->3)-beta-glucan endohydrolase OS=Cucumis melo OX=3656 GN=LOC103502975 PE=3 SV... [more]
A0A6J1I9Q32.4e-25791.00(1->3)-beta-glucan endohydrolase OS=Cucurbita maxima OX=3661 GN=LOC111472848 PE=... [more]
Match NameE-valueIdentityDescription
AT1G64760.17.5e-21175.70O-Glycosyl hydrolases family 17 protein [more]
AT1G64760.27.5e-21175.70O-Glycosyl hydrolases family 17 protein [more]
AT2G19440.11.2e-20573.57O-Glycosyl hydrolases family 17 protein [more]
AT3G04010.11.1e-19370.50O-Glycosyl hydrolases family 17 protein [more]
AT5G18220.13.3e-19069.09O-Glycosyl hydrolases family 17 protein [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (OHB3-1) v2
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR012946X8 domainSMARTSM00768X8_clscoord: 367..450
e-value: 4.9E-37
score: 139.0
IPR012946X8 domainPFAMPF07983X8coord: 367..437
e-value: 1.0E-17
score: 64.5
NoneNo IPR availableGENE3D1.20.58.1040coord: 365..457
e-value: 1.8E-8
score: 36.6
NoneNo IPR availableGENE3D3.20.20.80Glycosidasescoord: 23..353
e-value: 3.0E-110
score: 370.1
NoneNo IPR availablePANTHERPTHR32227:SF95GLUCAN ENDO-1,3-BETA-GLUCOSIDASE 8-RELATEDcoord: 8..460
IPR000490Glycoside hydrolase family 17PFAMPF00332Glyco_hydro_17coord: 24..345
e-value: 5.6E-67
score: 226.5
IPR000490Glycoside hydrolase family 17PROSITEPS00587GLYCOSYL_HYDROL_F17coord: 261..274
IPR044965Glycoside hydrolase family 17, plantPANTHERPTHR32227GLUCAN ENDO-1,3-BETA-GLUCOSIDASE BG1-RELATED-RELATEDcoord: 8..460
IPR017853Glycoside hydrolase superfamilySUPERFAMILY51445(Trans)glycosidasescoord: 24..345

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Moc05g14520.1Moc05g14520.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0005975 carbohydrate metabolic process
cellular_component GO:0046658 anchored component of plasma membrane
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0042973 glucan endo-1,3-beta-D-glucosidase activity
molecular_function GO:0004553 hydrolase activity, hydrolyzing O-glycosyl compounds