Moc05g14450 (gene) Bitter gourd (OHB3-1) v2

Overview


Name	Moc05g14450
Type	gene
Organism	Momordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
Description	Ribonuclease H
Location	chr5: 10927496 .. 10927888 (+)
RNA-Seq Expression	Moc05g14450
Synteny	Moc05g14450

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGACCTTCGCGTCCGAGGCCGTAGTGCCTGTAGAAGTTGGGATGCTAAAGGAGAGAGTAGACTACTATGAGCAGGCTAGGAATGAGGAAGAGTTACTCCTCAGCCTCGACCTCATCGATGAGAAAAGGGAAGCGGCCCAGCTGGGTTTGGCTGAATACCAAAATCGAATGGCAAGGCACCACAACTCGAGGGTCCAGCCTCAGAGCTTCCAGGTCAGACACCTAGTCCTTCGCAAGGTGTTGAAGCATGTTGAGGCGTTGGAACCAAGTTGGGAGGGGCCATTTGAAGTCAAGGGCATAGTCCGACCTGGGACGTATATGCTTGCCGATCTCGAAGGAAAGTTACTCTTGCACCCATGGAATGCAGAGCATTTGAAGAGATATTGCCCTTGA

mRNA sequence

ATGACCTTCGCGTCCGAGGCCGTAGTGCCTGTAGAAGTTGGGATGCTAAAGGAGAGAGTAGACTACTATGAGCAGGCTAGGAATGAGGAAGAGTTACTCCTCAGCCTCGACCTCATCGATGAGAAAAGGGAAGCGGCCCAGCTGGGTTTGGCTGAATACCAAAATCGAATGGCAAGGCACCACAACTCGAGGGTCCAGCCTCAGAGCTTCCAGGTCAGACACCTAGTCCTTCGCAAGGTGTTGAAGCATGTTGAGGCGTTGGAACCAAGTTGGGAGGGGCCATTTGAAGTCAAGGGCATAGTCCGACCTGGGACGTATATGCTTGCCGATCTCGAAGGAAAGTTACTCTTGCACCCATGGAATGCAGAGCATTTGAAGAGATATTGCCCTTGA

Coding sequence (CDS)

ATGACCTTCGCGTCCGAGGCCGTAGTGCCTGTAGAAGTTGGGATGCTAAAGGAGAGAGTAGACTACTATGAGCAGGCTAGGAATGAGGAAGAGTTACTCCTCAGCCTCGACCTCATCGATGAGAAAAGGGAAGCGGCCCAGCTGGGTTTGGCTGAATACCAAAATCGAATGGCAAGGCACCACAACTCGAGGGTCCAGCCTCAGAGCTTCCAGGTCAGACACCTAGTCCTTCGCAAGGTGTTGAAGCATGTTGAGGCGTTGGAACCAAGTTGGGAGGGGCCATTTGAAGTCAAGGGCATAGTCCGACCTGGGACGTATATGCTTGCCGATCTCGAAGGAAAGTTACTCTTGCACCCATGGAATGCAGAGCATTTGAAGAGATATTGCCCTTGA

Protein sequence

MTFASEAVVPVEVGMLKERVDYYEQARNEEELLLSLDLIDEKREAAQLGLAEYQNRMARHHNSRVQPQSFQVRHLVLRKVLKHVEALEPSWEGPFEVKGIVRPGTYMLADLEGKLLLHPWNAEHLKRYCP

Homology

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BLAST of Moc05g14450 vs. NCBI nr
Match: XP_022156070.1 (uncharacterized protein K02A2.6-like [Momordica charantia])

HSP 1 Score: 192.6 bits (488), Expect = 2.1e-45
Identity = 97/119 (81.51%), Postives = 107/119 (89.92%), Query Frame = 0


Query: 1   MTFASEAVVPVEVGMLKERVDYYEQARNEEELLLSLDLIDEKREAAQLGLAEYQNRMARH 60
           M F+SEAVV VE+GM  ERV+YYE+ RNEEELLL LDLIDEKREAAQL LAEYQN+MARH
Sbjct: 232 MAFSSEAVVLVEIGMPTERVEYYERVRNEEELLLYLDLIDEKREAAQLRLAEYQNQMARH 291

Query: 61  HNSRVQPQSFQVRHLVLRKVLKHVEALEPSWEGPFEVKGIVRPGTYMLADLEGKLLLHP 120
           +NSRV+ +SFQ+ +LVLRKV KHVE LEPSWEGPFEVKGIVRPGTYMLADLEGKLL HP
Sbjct: 292 YNSRVRSRSFQIGYLVLRKVQKHVEVLEPSWEGPFEVKGIVRPGTYMLADLEGKLLSHP 350

[Close]

BLAST of Moc05g14450 vs. NCBI nr
Match: XP_022159003.1 (uncharacterized protein LOC111025448 [Momordica charantia])

HSP 1 Score: 181.8 bits (460), Expect = 3.8e-42
Identity = 87/130 (66.92%), Postives = 103/130 (79.23%), Query Frame = 0


Query: 1   MTFASEAVVPVEVGMLKERVDYYEQARNEEELLLSLDLIDEKREAAQLGLAEYQNRMARH 60
           + F SEA+VP E+GM  +R++ YE  RNE ELLL+LDL++ KRE AQL L E QNRMARH
Sbjct: 500 LAFGSEAMVPAEIGMPTDRIEQYEPLRNEAELLLNLDLLEGKRETAQLRLVECQNRMARH 559

Query: 61  HNSRVQPQSFQVRHLVLRKVLKHVEALEPSWEGPFEVKGIVRPGTYMLADLEGKLLLHPW 120
           +N+R+QP+SFQV HLV  K+  HV  L+PSWE  FEVKGIVRPGTYMLADLE  +L HPW
Sbjct: 560 YNTRIQPRSFQVGHLVSSKIQSHVGTLDPSWEELFEVKGIVRPGTYMLADLERSVLAHPW 619

Query: 121 NAEHLKRYCP 131
           NAEHLKRY P
Sbjct: 620 NAEHLKRYYP 629

[Close]

BLAST of Moc05g14450 vs. NCBI nr
Match: XP_022156986.1 (uncharacterized protein LOC111023816 [Momordica charantia])

HSP 1 Score: 154.5 bits (389), Expect = 6.5e-34
Identity = 73/128 (57.03%), Postives = 100/128 (78.12%), Query Frame = 0


Query: 1   MTFASEAVVPVEVGMLKERVDYYEQARNEEELLLSLDLIDEKREAAQLGLAEYQNRMARH 60
           + F SEAVVP EVGM   RV+ +++  N E LL++LDL++EKR  +QL LAEYQNRMAR+
Sbjct: 716 LAFGSEAVVPAEVGMPTPRVENFDEQANSEALLVNLDLLEEKRSHSQLRLAEYQNRMARY 775

Query: 61  HNSRVQPQSFQVRHLVLRKVLKHVEALEPSWEGPFEVKGIVRPGTYMLADLEGKLLLHPW 120
           +N+RV+ ++F+   LVL+KV++HV ALEP+WEGP+ V  I+RP  Y+L+DL G+ L HPW
Sbjct: 776 YNARVKVRTFKPSDLVLKKVMQHVGALEPNWEGPYRVLKILRPRAYLLSDLNGRQLPHPW 835

Query: 121 NAEHLKRY 129
           NAEHL+ Y
Sbjct: 836 NAEHLRVY 843

[Close]

BLAST of Moc05g14450 vs. NCBI nr
Match: XP_022142775.1 (protein NYNRIN-like [Momordica charantia])

HSP 1 Score: 153.3 bits (386), Expect = 1.4e-33
Identity = 76/101 (75.25%), Postives = 85/101 (84.16%), Query Frame = 0


Query: 1   MTFASEAVVPVEVGMLKERVDYYEQARNEEELLLSLDLIDEKREAAQLGLAEYQNRMARH 60
           M F SEA+VPVE+GM  ER+DYYE  RNEEELLL+LDL+DEKRE AQL L EYQNR+ RH
Sbjct: 315 MAFGSEAMVPVEIGMPTERIDYYEHVRNEEELLLNLDLLDEKREVAQLRLTEYQNRITRH 374

Query: 61  HNSRVQPQSFQVRHLVLRKVLKHVEALEPSWEGPFEVKGIV 102
           +NS V+P+SFQV HLVLRKV KHV  LEPSWEG FEVKGIV
Sbjct: 375 YNSVVRPRSFQVGHLVLRKVQKHVNTLEPSWEGSFEVKGIV 415

[Close]

BLAST of Moc05g14450 vs. NCBI nr
Match: XP_022156049.1 (uncharacterized protein LOC111023017 [Momordica charantia])

HSP 1 Score: 146.4 bits (368), Expect = 1.8e-31
Identity = 69/128 (53.91%), Postives = 97/128 (75.78%), Query Frame = 0


Query: 1   MTFASEAVVPVEVGMLKERVDYYEQARNEEELLLSLDLIDEKREAAQLGLAEYQNRMARH 60
           + F SEA++PVE+GM   RV+ ++   N E  LL+LDL++EKR  +QL LAEYQNRMAR+
Sbjct: 62  LAFGSEAIIPVEIGMPTLRVENFDGQTNSEAFLLNLDLLEEKRSYSQLKLAEYQNRMARY 121

Query: 61  HNSRVQPQSFQVRHLVLRKVLKHVEALEPSWEGPFEVKGIVRPGTYMLADLEGKLLLHPW 120
           +N+R + ++F+   L L+KV++HVEALEP+WEGP+ V  +VRP  Y+L+DL G+ L HPW
Sbjct: 122 YNTRERVRTFKPGDLNLKKVMQHVEALEPNWEGPYRVLKVVRPRAYLLSDLNGRQLPHPW 181

Query: 121 NAEHLKRY 129
           NAE L+ Y
Sbjct: 182 NAEPLRVY 189

[Close]

BLAST of Moc05g14450 vs. ExPASy TrEMBL
Match: A0A6J1DP99 (uncharacterized protein K02A2.6-like OS=Momordica charantia OX=3673 GN=LOC111023037 PE=4 SV=1)

HSP 1 Score: 192.6 bits (488), Expect = 1.0e-45
Identity = 97/119 (81.51%), Postives = 107/119 (89.92%), Query Frame = 0


Query: 1   MTFASEAVVPVEVGMLKERVDYYEQARNEEELLLSLDLIDEKREAAQLGLAEYQNRMARH 60
           M F+SEAVV VE+GM  ERV+YYE+ RNEEELLL LDLIDEKREAAQL LAEYQN+MARH
Sbjct: 232 MAFSSEAVVLVEIGMPTERVEYYERVRNEEELLLYLDLIDEKREAAQLRLAEYQNQMARH 291

Query: 61  HNSRVQPQSFQVRHLVLRKVLKHVEALEPSWEGPFEVKGIVRPGTYMLADLEGKLLLHP 120
           +NSRV+ +SFQ+ +LVLRKV KHVE LEPSWEGPFEVKGIVRPGTYMLADLEGKLL HP
Sbjct: 292 YNSRVRSRSFQIGYLVLRKVQKHVEVLEPSWEGPFEVKGIVRPGTYMLADLEGKLLSHP 350

[Close]

BLAST of Moc05g14450 vs. ExPASy TrEMBL
Match: A0A6J1DXL9 (uncharacterized protein LOC111025448 OS=Momordica charantia OX=3673 GN=LOC111025448 PE=4 SV=1)

HSP 1 Score: 181.8 bits (460), Expect = 1.8e-42
Identity = 87/130 (66.92%), Postives = 103/130 (79.23%), Query Frame = 0


Query: 1   MTFASEAVVPVEVGMLKERVDYYEQARNEEELLLSLDLIDEKREAAQLGLAEYQNRMARH 60
           + F SEA+VP E+GM  +R++ YE  RNE ELLL+LDL++ KRE AQL L E QNRMARH
Sbjct: 500 LAFGSEAMVPAEIGMPTDRIEQYEPLRNEAELLLNLDLLEGKRETAQLRLVECQNRMARH 559

Query: 61  HNSRVQPQSFQVRHLVLRKVLKHVEALEPSWEGPFEVKGIVRPGTYMLADLEGKLLLHPW 120
           +N+R+QP+SFQV HLV  K+  HV  L+PSWE  FEVKGIVRPGTYMLADLE  +L HPW
Sbjct: 560 YNTRIQPRSFQVGHLVSSKIQSHVGTLDPSWEELFEVKGIVRPGTYMLADLERSVLAHPW 619

Query: 121 NAEHLKRYCP 131
           NAEHLKRY P
Sbjct: 620 NAEHLKRYYP 629

[Close]

BLAST of Moc05g14450 vs. ExPASy TrEMBL
Match: A0A6J1DWM2 (Ribonuclease H OS=Momordica charantia OX=3673 GN=LOC111023816 PE=4 SV=1)

HSP 1 Score: 154.5 bits (389), Expect = 3.1e-34
Identity = 73/128 (57.03%), Postives = 100/128 (78.12%), Query Frame = 0


Query: 1   MTFASEAVVPVEVGMLKERVDYYEQARNEEELLLSLDLIDEKREAAQLGLAEYQNRMARH 60
           + F SEAVVP EVGM   RV+ +++  N E LL++LDL++EKR  +QL LAEYQNRMAR+
Sbjct: 716 LAFGSEAVVPAEVGMPTPRVENFDEQANSEALLVNLDLLEEKRSHSQLRLAEYQNRMARY 775

Query: 61  HNSRVQPQSFQVRHLVLRKVLKHVEALEPSWEGPFEVKGIVRPGTYMLADLEGKLLLHPW 120
           +N+RV+ ++F+   LVL+KV++HV ALEP+WEGP+ V  I+RP  Y+L+DL G+ L HPW
Sbjct: 776 YNARVKVRTFKPSDLVLKKVMQHVGALEPNWEGPYRVLKILRPRAYLLSDLNGRQLPHPW 835

Query: 121 NAEHLKRY 129
           NAEHL+ Y
Sbjct: 836 NAEHLRVY 843

[Close]

BLAST of Moc05g14450 vs. ExPASy TrEMBL
Match: A0A6J1CLW5 (protein NYNRIN-like OS=Momordica charantia OX=3673 GN=LOC111012813 PE=4 SV=1)

HSP 1 Score: 153.3 bits (386), Expect = 7.0e-34
Identity = 76/101 (75.25%), Postives = 85/101 (84.16%), Query Frame = 0


Query: 1   MTFASEAVVPVEVGMLKERVDYYEQARNEEELLLSLDLIDEKREAAQLGLAEYQNRMARH 60
           M F SEA+VPVE+GM  ER+DYYE  RNEEELLL+LDL+DEKRE AQL L EYQNR+ RH
Sbjct: 315 MAFGSEAMVPVEIGMPTERIDYYEHVRNEEELLLNLDLLDEKREVAQLRLTEYQNRITRH 374

Query: 61  HNSRVQPQSFQVRHLVLRKVLKHVEALEPSWEGPFEVKGIV 102
           +NS V+P+SFQV HLVLRKV KHV  LEPSWEG FEVKGIV
Sbjct: 375 YNSVVRPRSFQVGHLVLRKVQKHVNTLEPSWEGSFEVKGIV 415

[Close]

BLAST of Moc05g14450 vs. ExPASy TrEMBL
Match: A0A2N9EW70 (Ribonuclease H OS=Fagus sylvatica OX=28930 GN=FSB_LOCUS6945 PE=4 SV=1)

HSP 1 Score: 146.7 bits (369), Expect = 6.5e-32
Identity = 72/135 (53.33%), Postives = 94/135 (69.63%), Query Frame = 0


Query: 1    MTFASEAVVPVEVGMLKERVDYYEQARNEEELLLSLDLIDEKREAAQLGLAEYQNRMARH 60
            MTF SEAVVPVE+G+   R   Y++ +NEE+L L+LDLIDE RE A+  +  YQ +MARH
Sbjct: 1419 MTFGSEAVVPVEIGLTTLRTSAYDEQQNEEQLRLNLDLIDEVRETAEARMKRYQEKMARH 1478

Query: 61   HNSRVQPQSFQVRHLVLRKVLKHVE-----ALEPSWEGPFEVKGIVRPGTYMLADLEGKL 120
            HNS+V+P+   +  LVLRKV    +      L P+WEGP++V  I RPGTY L D+ G+ 
Sbjct: 1479 HNSKVKPRQLSIGDLVLRKVTLATKNPSEGKLGPNWEGPYKVIEIRRPGTYHLQDMNGRR 1538

Query: 121  LLHPWNAEHLKRYCP 131
            L HPWNAEHL++Y P
Sbjct: 1539 LPHPWNAEHLRKYYP 1553

The following BLAST results are available for this feature:

BLAST of Moc05g14450 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022156070.1	2.1e-45	81.51	uncharacterized protein K02A2.6-like [Momordica charantia]	[more]
XP_022159003.1	3.8e-42	66.92	uncharacterized protein LOC111025448 [Momordica charantia]	[more]
XP_022156986.1	6.5e-34	57.03	uncharacterized protein LOC111023816 [Momordica charantia]	[more]
XP_022142775.1	1.4e-33	75.25	protein NYNRIN-like [Momordica charantia]	[more]
XP_022156049.1	1.8e-31	53.91	uncharacterized protein LOC111023017 [Momordica charantia]	[more]

BLAST of Moc05g14450 vs. NCBI nr


Match Name	E-value	Identity	Description

BLAST of Moc05g14450 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1DP99	1.0e-45	81.51	uncharacterized protein K02A2.6-like OS=Momordica charantia OX=3673 GN=LOC111023...	[more]
A0A6J1DXL9	1.8e-42	66.92	uncharacterized protein LOC111025448 OS=Momordica charantia OX=3673 GN=LOC111025...	[more]
A0A6J1DWM2	3.1e-34	57.03	Ribonuclease H OS=Momordica charantia OX=3673 GN=LOC111023816 PE=4 SV=1	[more]
A0A6J1CLW5	7.0e-34	75.25	protein NYNRIN-like OS=Momordica charantia OX=3673 GN=LOC111012813 PE=4 SV=1	[more]
A0A2N9EW70	6.5e-32	53.33	Ribonuclease H OS=Fagus sylvatica OX=28930 GN=FSB_LOCUS6945 PE=4 SV=1	[more]

BLAST of Moc05g14450 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Moc05g14450.1	Moc05g14450.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0015074	DNA integration
cellular_component	GO:0110165	cellular anatomical entity
molecular_function	GO:0140640	catalytic activity, acting on a nucleic acid
molecular_function	GO:0003676	nucleic acid binding