Moc05g10710 (gene) Bitter gourd (OHB3-1) v2

Overview


Name	Moc05g10710
Type	gene
Organism	Momordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
Description	Transposon TX1 uncharacterized 149 kDa protein
Location	chr5: 8444705 .. 8445424 (-)
RNA-Seq Expression	Moc05g10710
Synteny	Moc05g10710

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAAACACTTGCTGCCACACACCATATCCGATGAACAATCAGCTTTTGTGGCCGGAGGACAGATTCTTGATGCATCTCTAATCGCCAACGAGCTTATTGAGGAATGGGATAGACAACAAAAGAAGGGAGCGGTTATTAAGCTGGATATAGAGAAGGCTTTCGATAAGGTTGACTGGGATTTTCTAGATGATTTACTCAAAGCTAAAGGATTTGGTGACACTTGGAGAAAATAGATCCGGGGTTGTGTATCATCAGCAAACTTCTTCATCATTATAAATGGCCGGCCTAGAGGGAAAATCATCGCTTCTAGAGGCCTCCGACAAGGGGAACCTTTATCACCTTTTCTCTTTATTATAGTGGTTGATGTCTTTAGCCGCCTCATGTCTCTATCAGCTAGGAACAATCACATTAGGGGCTTTGATCTTGGGGAGAACTCATTGAACATCCAACACCTACAATTTGTTGATGACACTATCCTTTTCTCCACATATGACGATTTCTCCCTCAAAAACCTATTAAGTGTTATTCGATCATTTGAAGAAGCATCAAGGCTTAATGTCAACAAACAGAAATCTGAAATTTTGGGAATCAACCTAACCGACATCAAGCACTTTACCATAGCAGCAATGTATTCTGTAAAAAAGGATCCTGGCCTACATCCTACCTCGGTCTTCCCCTTCATGGCAAGCCTAGAACTCTCTCTTTCTGGGACCCTATGA

mRNA sequence

ATGAAACACTTGCTGCCACACACCATATCCGATGAACAATCAGCTTTTGTGGCCGGAGGACAGATTCTTGATGCATCTCTAATCGCCAACGAGCTTATTGAGGAATGGGATAGACAACAAAAGAAGGGAGCGGTTATTAAGCTGGATATAGAGAAGGCTTTCGATAAGGTTGACTGGGATTTTCTAGATGATTTACTCAAAGCTAAAGGATTTGCTAGGAACAATCACATTAGGGGCTTTGATCTTGGGGAGAACTCATTGAACATCCAACACCTACAATTTGTTGATGACACTATCCTTTTCTCCACATATGACGATTTCTCCCTCAAAAACCTATTAAGTGTTATTCGATCATTTGAAGAAGCATCAAGGCTTAATGTCAACAAACAGAAATCTGAAATTTTGGGAATCAACCTAACCGACATCAAGCACTTTACCATAGCAGCAATGTATTCTGTAAAAAAGGATCCTGGCCTACATCCTACCTCGGTCTTCCCCTTCATGGCAAGCCTAGAACTCTCTCTTTCTGGGACCCTATGA

Coding sequence (CDS)

ATGAAACACTTGCTGCCACACACCATATCCGATGAACAATCAGCTTTTGTGGCCGGAGGACAGATTCTTGATGCATCTCTAATCGCCAACGAGCTTATTGAGGAATGGGATAGACAACAAAAGAAGGGAGCGGTTATTAAGCTGGATATAGAGAAGGCTTTCGATAAGGTTGACTGGGATTTTCTAGATGATTTACTCAAAGCTAAAGGATTTGCTAGGAACAATCACATTAGGGGCTTTGATCTTGGGGAGAACTCATTGAACATCCAACACCTACAATTTGTTGATGACACTATCCTTTTCTCCACATATGACGATTTCTCCCTCAAAAACCTATTAAGTGTTATTCGATCATTTGAAGAAGCATCAAGGCTTAATGTCAACAAACAGAAATCTGAAATTTTGGGAATCAACCTAACCGACATCAAGCACTTTACCATAGCAGCAATGTATTCTGTAAAAAAGGATCCTGGCCTACATCCTACCTCGGTCTTCCCCTTCATGGCAAGCCTAGAACTCTCTCTTTCTGGGACCCTATGA

Protein sequence

MKHLLPHTISDEQSAFVAGGQILDASLIANELIEEWDRQQKKGAVIKLDIEKAFDKVDWDFLDDLLKAKGFARNNHIRGFDLGENSLNIQHLQFVDDTILFSTYDDFSLKNLLSVIRSFEEASRLNVNKQKSEILGINLTDIKHFTIAAMYSVKKDPGLHPTSVFPFMASLELSLSGTL

Homology

[Close]

BLAST of Moc05g10710 vs. NCBI nr
Match: XP_022145825.1 (uncharacterized protein LOC111015185 [Momordica charantia])

HSP 1 Score: 346.7 bits (888), Expect = 1.2e-91
Identity = 179/191 (93.72%), Postives = 179/191 (93.72%), Query Frame = 0


Query: 1   MKHLLPHTISDEQSAFVAGGQILDASLIANELIEEWDRQQKKGAVIKLDIEKAFDKVDWD 60
           MKHLLPHTISDEQSAFVAGGQILDASLIANELIEEWDRQQKKGAVIKLDIEKAFDKVDWD
Sbjct: 1   MKHLLPHTISDEQSAFVAGGQILDASLIANELIEEWDRQQKKGAVIKLDIEKAFDKVDWD 60

Query: 61  FLDDLLKAKGF------------ARNNHIRGFDLGENSLNIQHLQFVDDTILFSTYDDFS 120
           FLDDLLKAKGF            ARNNHIRGFDLGENSLNIQHLQFVDDTILFSTYDDFS
Sbjct: 61  FLDDLLKAKGFVVDVFSRLMSLSARNNHIRGFDLGENSLNIQHLQFVDDTILFSTYDDFS 120

Query: 121 LKNLLSVIRSFEEASRLNVNKQKSEILGINLTDIKHFTIAAMYSVKKDPGLHPTSVFPFM 180
           LKNLLSVIRSFEEASRLNVNKQKSEILGINLTDIKHFTIAAMYSVKKDPGLHPTSVFPFM
Sbjct: 121 LKNLLSVIRSFEEASRLNVNKQKSEILGINLTDIKHFTIAAMYSVKKDPGLHPTSVFPFM 180

[Close]

BLAST of Moc05g10710 vs. NCBI nr
Match: XP_022132189.1 (uncharacterized protein LOC111005109 [Momordica charantia])

HSP 1 Score: 171.4 bits (433), Expect = 7.0e-39
Identity = 91/158 (57.59%), Postives = 107/158 (67.72%), Query Frame = 0


Query: 1   MKHLLPHTISDEQSAFVAGGQILDASLIANELIEEWDRQQKKGAVIKLDIEKAFDKVDWD 60
           MK LLPHTI+ EQSAFVAG QILDASLIANE+IE+WDR+++KG VIKLDIEKAFDKVDWD
Sbjct: 1   MKSLLPHTITKEQSAFVAGRQILDASLIANEIIEDWDRKKRKGVVIKLDIEKAFDKVDWD 60

Query: 61  FLDDLLKAKGF------------------------------ARNNHIRGFDLGENSLNIQ 120
           FLD++L AKGF                              A     +GFD+G NSL I 
Sbjct: 61  FLDEVLAAKGFRHTWRKWIWGCISTTNFSIIINGRPRGKRTASRGLRQGFDVGNNSLAIH 120

Query: 121 HLQFVDDTILFSTYDDFSLKNLLSVIRSFEEASRLNVN 129
           HLQF DDTIL S YD+ SL N+ ++IR FEEA+ LN+N
Sbjct: 121 HLQFADDTILLSPYDNLSLTNMFNIIRLFEEATGLNIN 158

[Close]

BLAST of Moc05g10710 vs. NCBI nr
Match: RVW19142.1 (Transposon TX1 uncharacterized 149 kDa protein [Vitis vinifera])

HSP 1 Score: 120.6 bits (301), Expect = 1.4e-23
Identity = 72/191 (37.70%), Postives = 97/191 (50.79%), Query Frame = 0


Query: 1   MKHLLPHTISDEQSAFVAGGQILDASLIANELIEEWDRQQKKGAVIKLDIEKAFDKVDWD 60
           ++ +L  TI   Q AFV G QI+DA LIANE+++E  R  ++G V K+D EKA+D V WD
Sbjct: 459 LRGVLHETIHSTQGAFVQGRQIMDAVLIANEIVDERRRSGEEGVVFKIDFEKAYDHVRWD 518

Query: 61  FLDDLLKAKGFA--------------------------RNNHIRGFDLGENSLNIQHLQF 120
           FLD +L+ KGF+                            N + GF +G N   + HLQF
Sbjct: 519 FLDQVLEKKGFSPKWRKWMNGCLSSVSYAVLVNGSVKEERNMLEGFRVGRNRTRVSHLQF 578

Query: 121 VDDTILFSTYDDFSLKNLLSVIRSFEEASRLNVNKQKSEILGINLTDIKHFTIAAMYSVK 166
            DDTI FS   +  L+ L S++ +F   S L VN  KS I GINL       +A     +
Sbjct: 579 ADDTIFFSNTREEDLQTLKSLLLAFGHISGLKVNLDKSNIYGINLDQAHISRLAETLDAR 638

[Close]

BLAST of Moc05g10710 vs. NCBI nr
Match: XP_028119202.1 (uncharacterized protein LOC114316718 [Camellia sinensis])

HSP 1 Score: 120.2 bits (300), Expect = 1.9e-23
Identity = 62/146 (42.47%), Postives = 87/146 (59.59%), Query Frame = 0


Query: 1   MKHLLPHTISDEQSAFVAGGQILDASLIANELIEEWDRQQKKGAVIKLDIEKAFDKVDWD 60
           MK ++P  IS+ QSAF+ G  ILD  LIANE+++ W + +KKG  +KLD EKA+D V+WD
Sbjct: 348 MKQVMPMIISEAQSAFLGGRNILDGILIANEIVDGWRKSKKKGLALKLDFEKAYDSVNWD 407

Query: 61  FLDDLLKAKGFA-----RNNHIRGFDLGENSLNIQHLQFVDDTILFSTYDDFSLKNLLSV 120
           FL ++    GF          I+G  +G N + I HLQF DDTILF   +   +  +  +
Sbjct: 408 FLFNMSSNFGFGDRWAKEMGLIKGVVIGNNGVTIMHLQFADDTILFCEAEWVEVVTIKRI 467

Query: 121 IRSFEEASRLNVNKQKSEILGINLTD 142
           +R FE  S L +N  KS I G+ + D
Sbjct: 468 LRCFEILSGLKINYHKSVICGVGVDD 493

[Close]

BLAST of Moc05g10710 vs. NCBI nr
Match: RVX05736.1 (Transposon TX1 uncharacterized 149 kDa protein [Vitis vinifera])

HSP 1 Score: 119.8 bits (299), Expect = 2.4e-23
Identity = 73/191 (38.22%), Postives = 94/191 (49.21%), Query Frame = 0


Query: 1    MKHLLPHTISDEQSAFVAGGQILDASLIANELIEEWDRQQKKGAVIKLDIEKAFDKVDWD 60
            ++ +L  TI   Q AFV G QILDA LIANE+++E  R  ++G V K+D EKA+D V WD
Sbjct: 835  LRGVLHETIHYTQGAFVQGRQILDAVLIANEIVDERRRSGEEGVVFKIDFEKAYDHVKWD 894

Query: 61   FLDDLLKAKGFA--------------------------------RNNHIRGFDLGENSLN 120
            FLD +L+ KGF+                                  N + GF +G N   
Sbjct: 895  FLDHVLEKKGFSPRWRKWMSGCLSSVSYAILVNGSAKGMLMRAEERNMMEGFRVGRNRTR 954

Query: 121  IQHLQFVDDTILFSTYDDFSLKNLLSVIRSFEEASRLNVNKQKSEILGINLTDIKHFTIA 160
            + HLQF DDTI FS   +  L+ L S++  F   S L VN  KS I GINL       +A
Sbjct: 955  VSHLQFADDTIFFSNSREEELQTLKSLLLVFGHISGLKVNLNKSSIYGINLDQAHLSRLA 1014

[Close]

BLAST of Moc05g10710 vs. ExPASy TrEMBL
Match: A0A6J1CVL8 (uncharacterized protein LOC111015185 OS=Momordica charantia OX=3673 GN=LOC111015185 PE=4 SV=1)

HSP 1 Score: 346.7 bits (888), Expect = 5.9e-92
Identity = 179/191 (93.72%), Postives = 179/191 (93.72%), Query Frame = 0


Query: 1   MKHLLPHTISDEQSAFVAGGQILDASLIANELIEEWDRQQKKGAVIKLDIEKAFDKVDWD 60
           MKHLLPHTISDEQSAFVAGGQILDASLIANELIEEWDRQQKKGAVIKLDIEKAFDKVDWD
Sbjct: 1   MKHLLPHTISDEQSAFVAGGQILDASLIANELIEEWDRQQKKGAVIKLDIEKAFDKVDWD 60

Query: 61  FLDDLLKAKGF------------ARNNHIRGFDLGENSLNIQHLQFVDDTILFSTYDDFS 120
           FLDDLLKAKGF            ARNNHIRGFDLGENSLNIQHLQFVDDTILFSTYDDFS
Sbjct: 61  FLDDLLKAKGFVVDVFSRLMSLSARNNHIRGFDLGENSLNIQHLQFVDDTILFSTYDDFS 120

Query: 121 LKNLLSVIRSFEEASRLNVNKQKSEILGINLTDIKHFTIAAMYSVKKDPGLHPTSVFPFM 180
           LKNLLSVIRSFEEASRLNVNKQKSEILGINLTDIKHFTIAAMYSVKKDPGLHPTSVFPFM
Sbjct: 121 LKNLLSVIRSFEEASRLNVNKQKSEILGINLTDIKHFTIAAMYSVKKDPGLHPTSVFPFM 180

[Close]

BLAST of Moc05g10710 vs. ExPASy TrEMBL
Match: A0A6J1BSD3 (uncharacterized protein LOC111005109 OS=Momordica charantia OX=3673 GN=LOC111005109 PE=4 SV=1)

HSP 1 Score: 171.4 bits (433), Expect = 3.4e-39
Identity = 91/158 (57.59%), Postives = 107/158 (67.72%), Query Frame = 0


Query: 1   MKHLLPHTISDEQSAFVAGGQILDASLIANELIEEWDRQQKKGAVIKLDIEKAFDKVDWD 60
           MK LLPHTI+ EQSAFVAG QILDASLIANE+IE+WDR+++KG VIKLDIEKAFDKVDWD
Sbjct: 1   MKSLLPHTITKEQSAFVAGRQILDASLIANEIIEDWDRKKRKGVVIKLDIEKAFDKVDWD 60

Query: 61  FLDDLLKAKGF------------------------------ARNNHIRGFDLGENSLNIQ 120
           FLD++L AKGF                              A     +GFD+G NSL I 
Sbjct: 61  FLDEVLAAKGFRHTWRKWIWGCISTTNFSIIINGRPRGKRTASRGLRQGFDVGNNSLAIH 120

Query: 121 HLQFVDDTILFSTYDDFSLKNLLSVIRSFEEASRLNVN 129
           HLQF DDTIL S YD+ SL N+ ++IR FEEA+ LN+N
Sbjct: 121 HLQFADDTILLSPYDNLSLTNMFNIIRLFEEATGLNIN 158

[Close]

BLAST of Moc05g10710 vs. ExPASy TrEMBL
Match: A0A803PZS1 (Uncharacterized protein OS=Cannabis sativa OX=3483 PE=4 SV=1)

HSP 1 Score: 122.9 bits (307), Expect = 1.4e-24
Identity = 71/160 (44.38%), Postives = 96/160 (60.00%), Query Frame = 0


Query: 1   MKHLLPHTISDEQSAFVAGGQILDASLIANELIEEWDRQQKKGAVIKLDIEKAFDKVDWD 60
           ++ +L  TIS+ Q AFV G QILD+ LIANE +EE+  + KKG V K+D+EKA+D VDWD
Sbjct: 230 LRGVLGETISETQLAFVEGRQILDSVLIANETVEEYRSRGKKGFVFKIDLEKAYDCVDWD 289

Query: 61  FLDDLLKAKGFAR-------------------NNHIRGFDLGENSLNIQHLQFVDDTILF 120
           FLD +LK KGF                     N   RG   G++++ I HLQF DDT LF
Sbjct: 290 FLDLVLKEKGFGELWRKWIRGCVSSTLFSLLINGRARGKFRGKDNVQISHLQFADDT-LF 349

Query: 121 STYDDFSLKNLLSVIRSFEEASRLNVNKQKSEILGINLTD 142
              D+ SL+ L+ ++ +F   S L VN  KS++LGI L +
Sbjct: 350 FVKDEASLRKLVEIVETFCGVSSLKVNLNKSQLLGICLEE 388

[Close]

BLAST of Moc05g10710 vs. ExPASy TrEMBL
Match: A0A438C7C8 (Transposon TX1 uncharacterized 149 kDa protein OS=Vitis vinifera OX=29760 GN=YTX2_410 PE=4 SV=1)

HSP 1 Score: 120.6 bits (301), Expect = 6.9e-24
Identity = 72/191 (37.70%), Postives = 97/191 (50.79%), Query Frame = 0


Query: 1   MKHLLPHTISDEQSAFVAGGQILDASLIANELIEEWDRQQKKGAVIKLDIEKAFDKVDWD 60
           ++ +L  TI   Q AFV G QI+DA LIANE+++E  R  ++G V K+D EKA+D V WD
Sbjct: 459 LRGVLHETIHSTQGAFVQGRQIMDAVLIANEIVDERRRSGEEGVVFKIDFEKAYDHVRWD 518

Query: 61  FLDDLLKAKGFA--------------------------RNNHIRGFDLGENSLNIQHLQF 120
           FLD +L+ KGF+                            N + GF +G N   + HLQF
Sbjct: 519 FLDQVLEKKGFSPKWRKWMNGCLSSVSYAVLVNGSVKEERNMLEGFRVGRNRTRVSHLQF 578

Query: 121 VDDTILFSTYDDFSLKNLLSVIRSFEEASRLNVNKQKSEILGINLTDIKHFTIAAMYSVK 166
            DDTI FS   +  L+ L S++ +F   S L VN  KS I GINL       +A     +
Sbjct: 579 ADDTIFFSNTREEDLQTLKSLLLAFGHISGLKVNLDKSNIYGINLDQAHISRLAETLDAR 638

[Close]

BLAST of Moc05g10710 vs. ExPASy TrEMBL
Match: A0A438JA13 (Transposon TX1 uncharacterized 149 kDa protein OS=Vitis vinifera OX=29760 GN=YTX2_396 PE=4 SV=1)

HSP 1 Score: 119.8 bits (299), Expect = 1.2e-23
Identity = 73/191 (38.22%), Postives = 94/191 (49.21%), Query Frame = 0


Query: 1    MKHLLPHTISDEQSAFVAGGQILDASLIANELIEEWDRQQKKGAVIKLDIEKAFDKVDWD 60
            ++ +L  TI   Q AFV G QILDA LIANE+++E  R  ++G V K+D EKA+D V WD
Sbjct: 835  LRGVLHETIHYTQGAFVQGRQILDAVLIANEIVDERRRSGEEGVVFKIDFEKAYDHVKWD 894

Query: 61   FLDDLLKAKGFA--------------------------------RNNHIRGFDLGENSLN 120
            FLD +L+ KGF+                                  N + GF +G N   
Sbjct: 895  FLDHVLEKKGFSPRWRKWMSGCLSSVSYAILVNGSAKGMLMRAEERNMMEGFRVGRNRTR 954

Query: 121  IQHLQFVDDTILFSTYDDFSLKNLLSVIRSFEEASRLNVNKQKSEILGINLTDIKHFTIA 160
            + HLQF DDTI FS   +  L+ L S++  F   S L VN  KS I GINL       +A
Sbjct: 955  VSHLQFADDTIFFSNSREEELQTLKSLLLVFGHISGLKVNLNKSSIYGINLDQAHLSRLA 1014

[Close]

BLAST of Moc05g10710 vs. TAIR 10
Match: AT4G20520.1 (RNA binding;RNA-directed DNA polymerases )

HSP 1 Score: 50.8 bits (120), Expect = 1.3e-06
Identity = 24/73 (32.88%), Postives = 43/73 (58.90%), Query Frame = 0


Query: 1  MKHLLPHTISDEQSAFVAGGQILDASLIANELIEEWDRQQ--KKGAVIKLDIEKAFDKVD 60
          +K L+ + I   Q++F+ G    D  +   E +    R++  K   ++KLD+EKA+D++ 
Sbjct: 5  LKPLMTNLIGPAQASFIPGRVSTDNIVFVQEAVHSMRRKKGVKGWMLLKLDLEKAYDRIR 64

Query: 61 WDFLDDLLKAKGF 72
          WD+L+D L + GF
Sbjct: 65 WDYLEDTLISAGF 77

The following BLAST results are available for this feature:

BLAST of Moc05g10710 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022145825.1	1.2e-91	93.72	uncharacterized protein LOC111015185 [Momordica charantia]	[more]
XP_022132189.1	7.0e-39	57.59	uncharacterized protein LOC111005109 [Momordica charantia]	[more]
RVW19142.1	1.4e-23	37.70	Transposon TX1 uncharacterized 149 kDa protein [Vitis vinifera]	[more]
XP_028119202.1	1.9e-23	42.47	uncharacterized protein LOC114316718 [Camellia sinensis]	[more]
RVX05736.1	2.4e-23	38.22	Transposon TX1 uncharacterized 149 kDa protein [Vitis vinifera]	[more]

BLAST of Moc05g10710 vs. NCBI nr


Match Name	E-value	Identity	Description

BLAST of Moc05g10710 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1CVL8	5.9e-92	93.72	uncharacterized protein LOC111015185 OS=Momordica charantia OX=3673 GN=LOC111015...	[more]
A0A6J1BSD3	3.4e-39	57.59	uncharacterized protein LOC111005109 OS=Momordica charantia OX=3673 GN=LOC111005...	[more]
A0A803PZS1	1.4e-24	44.38	Uncharacterized protein OS=Cannabis sativa OX=3483 PE=4 SV=1	[more]
A0A438C7C8	6.9e-24	37.70	Transposon TX1 uncharacterized 149 kDa protein OS=Vitis vinifera OX=29760 GN=YTX...	[more]
A0A438JA13	1.2e-23	38.22	Transposon TX1 uncharacterized 149 kDa protein OS=Vitis vinifera OX=29760 GN=YTX...	[more]

BLAST of Moc05g10710 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G20520.1	1.3e-06	32.88	RNA binding;RNA-directed DNA polymerases	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (OHB3-1) v2
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000477	Reverse transcriptase domain	PFAM	PF00078	RVT_1	coord: 4..72 e-value: 1.6E-9 score: 37.7
None	No IPR available	PANTHER	PTHR33116	REVERSE TRANSCRIPTASE ZINC-BINDING DOMAIN-CONTAINING PROTEIN-RELATED-RELATED	coord: 1..139
None	No IPR available	PANTHER	PTHR33116:SF33	OS01G0885550 PROTEIN	coord: 1..139

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Moc05g10710.1	Moc05g10710.1	mRNA