Moc05g09520 (gene) Bitter gourd (OHB3-1) v2

Overview
NameMoc05g09520
Typegene
OrganismMomordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
DescriptionRetrovirus-related Pol polyprotein from transposon TNT 1-94
Locationchr5: 7379405 .. 7380982 (-)
RNA-Seq ExpressionMoc05g09520
SyntenyMoc05g09520
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGGAATTGGTGAGCAGTCGAGTTTCGAAGGAATTATGAGGTTCGATGGAGCCAACTTTGGGTATTGGAAAATGCAGATTAAGGATTACTTAACTTGCAAAAAGGTGCACAAAGCGTTGAAGGAACGACCAGCTGGAATGAAGCTTGAAGATTGGGAAGATATGGATGAACAGGCAGTTGCAATTATTAGGTTGTGTCTATCAATGAATGTAGCAAGTCTCGTGGCAAATGAGACAACAGCGATAGGTCTGATGAATGCACTGACCAACATGTATGAAAAATAATCTGCCAATAATAAGGTATATCTTGTTAGAAGATATTTTAACATTCGTATGGATGAGAATGCTTCTGTGAATTCCCATATAAATGAAGTCACTAATTTAATCAATCAGTTAGCGTCTCTGAAGATTACATTTAGTGATGAGGTGAATGCTATTTTATTGTTGACTTCTTTGCCTGAAAGTTGGGAAACAATGAAGACAGCTGTGTCTAATTCATTAGGAGATAAGAGTTTAAAATTTACAGAAATTTGTGATGCTGCAACTGCTGAGGAAATTCGCAGGAAAGAGAGTGGAAAAGAATCCACTTCTAGTTCTGCATCTGGCTCAGCATTAGCTGTTCAGAGAGGTAAAGAAAAGGTTGAGTATGATGACAGACAACATAGAAATAGCAGGAAAAATGGGAATGGTGAGATTGAATGTTATTACTGCCACAAGAAGGGCCACTATAAGAGGCACTGTAGAAAATTGAAAGAGGATCTGAAAAAGCTGGATGCAAATGCAAATGCAGTTGTGCATGATGCTCTTGTGTGTGTTGAGAGTAACACAATGTCGGTGGACCAGTCACCAGAATGGATAGTAGACAGTGTAGCTTCAGTACACATCTCTTCAGATAGGAGGTTGTTCACATCTTTCACTGCAGAAAACTATGGCATGGTGAGGATGGGAAATGGTAGGCTCTCCAAGACTAGAGGAATTGGAAATATCAGTCTGAAGACCGATAGTGGGACTGAGTTGGTTTTGCGAGATGTTAGGTATGTACCCAGCATCAGAATGAATTTAATATCTGCAGGGAAGTTGGATGACGATGGTTACAGAAGTGAGTTTGCTGGAAATATGTGGAAACTCACTAGAGGATCCGAGCTGGTGGTTGTTGGCCACAGGACATCTACAGTGTACACAGTGAGGTTTGATGTTGCCAAAGGATCAGAGAGACAGAGGATGCATAGAGCTGCAAATGGTTTAGATGGAGACGGGATAAAACCAACAGCATTGACAACCAAGATAGATCCATCAGTTCATGTTCAACAATCGGGAGGCAAGGATAAGGAAAAGAAAAAGAGTTTAGTTGGTTGTGAGGTGATAGCCCCAGTTGTCAGACGGTTCAACGAATTGATGAAGTCGCATAGGCGAAAAGATGCATCGAAGAGGAAGACTACAGTTGGTGCTGAGGTCGAGGCTGAAGTCTCTAGCTTGGTAACAGACTTAAGTGGGAGTGTCAAGTCATCGAAGAAGAATTCTTTCTTTGGGAGTCGTTGGCCACGATCGAAGAAGGAAGCTTTGGGAGGTACCACTTAG

mRNA sequence

ATGGGAATTGGTGAGCAGTCGAGTTTCGAAGGAATTATGAGGTTCGATGGAGCCAACTTTGGGTATTGGAAAATGCAGATTAAGGATTACTTAACTTGCAAAAAGGTGCACAAAGCGTTGAAGGAACGACCAGCTGGAATGAAGCTTGAAGATTGGGAAGATATGGATGAACAGGCAGTTGCAATTATTAGGTTGTGTCTATCAATGAATGTAGCAAGTCTCGTGGCAAATGAGACAACAGCGATAGCGTCTCTGAAGATTACATTTAGTGATGAGGTGAATGCTATTTTATTGTTGACTTCTTTGCCTGAAAGTTGGGAAACAATGAAGACAGCTGTGTCTAATTCATTAGGAGATAAGAGTTTAAAATTTACAGAAATTTGTGATGCTGCAACTGCTGAGGAAATTCGCAGGAAAGAGAGTGGAAAAGAATCCACTTCTAGTTCTGCATCTGGCTCAGCATTAGCTGTTCAGAGAGGTAAAGAAAAGGTTGAGTATGATGACAGACAACATAGAAATAGCAGGAAAAATGGGAATGGTGAGATTGAATGTTATTACTGCCACAAGAAGGGCCACTATAAGAGGCACTGTAGAAAATTGAAAGAGGATCTGAAAAAGCTGGATGCAAATGCAAATGCAGTTGTGCATGATGCTCTTGTGTGTGTTGAGAGTAACACAATGTCGGTGGACCAGTCACCAGAATGGATAGTAGACAGTGTAGCTTCAGTACACATCTCTTCAGATAGGAGGTTGTTCACATCTTTCACTGCAGAAAACTATGGCATGGTGAGGATGGGAAATGGTAGGCTCTCCAAGACTAGAGGAATTGGAAATATCAGTCTGAAGACCGATAGTGGGACTGAGTTGGTTTTGCGAGATGTTAGGTATGTACCCAGCATCAGAATGAATTTAATATCTGCAGGGAAGTTGGATGACGATGGTTACAGAAGTGAGTTTGCTGGAAATATGTGGAAACTCACTAGAGGATCCGAGCTGGTGGTTGTTGGCCACAGGACATCTACAGTGTACACAGTGAGGTTTGATGTTGCCAAAGGATCAGAGAGACAGAGGATGCATAGAGCTGCAAATGGTTTAGATGGAGACGGGATAAAACCAACAGCATTGACAACCAAGATAGATCCATCAGTTCATGTTCAACAATCGGGAGGCAAGGATAAGGAAAAGAAAAAGAGTTTAGTTGGTTGTGAGGTGATAGCCCCAGTTGTCAGACGGTTCAACGAATTGATGAAGTCGCATAGGCGAAAAGATGCATCGAAGAGGAAGACTACAGTTGGTGCTGAGGTCGAGGCTGAAGTCTCTAGCTTGGTAACAGACTTAAGTGGGAGTGTCAAGTCATCGAAGAAGAATTCTTTCTTTGGGAGTCGTTGGCCACGATCGAAGAAGGAAGCTTTGGGAGGTACCACTTAG

Coding sequence (CDS)

ATGGGAATTGGTGAGCAGTCGAGTTTCGAAGGAATTATGAGGTTCGATGGAGCCAACTTTGGGTATTGGAAAATGCAGATTAAGGATTACTTAACTTGCAAAAAGGTGCACAAAGCGTTGAAGGAACGACCAGCTGGAATGAAGCTTGAAGATTGGGAAGATATGGATGAACAGGCAGTTGCAATTATTAGGTTGTGTCTATCAATGAATGTAGCAAGTCTCGTGGCAAATGAGACAACAGCGATAGCGTCTCTGAAGATTACATTTAGTGATGAGGTGAATGCTATTTTATTGTTGACTTCTTTGCCTGAAAGTTGGGAAACAATGAAGACAGCTGTGTCTAATTCATTAGGAGATAAGAGTTTAAAATTTACAGAAATTTGTGATGCTGCAACTGCTGAGGAAATTCGCAGGAAAGAGAGTGGAAAAGAATCCACTTCTAGTTCTGCATCTGGCTCAGCATTAGCTGTTCAGAGAGGTAAAGAAAAGGTTGAGTATGATGACAGACAACATAGAAATAGCAGGAAAAATGGGAATGGTGAGATTGAATGTTATTACTGCCACAAGAAGGGCCACTATAAGAGGCACTGTAGAAAATTGAAAGAGGATCTGAAAAAGCTGGATGCAAATGCAAATGCAGTTGTGCATGATGCTCTTGTGTGTGTTGAGAGTAACACAATGTCGGTGGACCAGTCACCAGAATGGATAGTAGACAGTGTAGCTTCAGTACACATCTCTTCAGATAGGAGGTTGTTCACATCTTTCACTGCAGAAAACTATGGCATGGTGAGGATGGGAAATGGTAGGCTCTCCAAGACTAGAGGAATTGGAAATATCAGTCTGAAGACCGATAGTGGGACTGAGTTGGTTTTGCGAGATGTTAGGTATGTACCCAGCATCAGAATGAATTTAATATCTGCAGGGAAGTTGGATGACGATGGTTACAGAAGTGAGTTTGCTGGAAATATGTGGAAACTCACTAGAGGATCCGAGCTGGTGGTTGTTGGCCACAGGACATCTACAGTGTACACAGTGAGGTTTGATGTTGCCAAAGGATCAGAGAGACAGAGGATGCATAGAGCTGCAAATGGTTTAGATGGAGACGGGATAAAACCAACAGCATTGACAACCAAGATAGATCCATCAGTTCATGTTCAACAATCGGGAGGCAAGGATAAGGAAAAGAAAAAGAGTTTAGTTGGTTGTGAGGTGATAGCCCCAGTTGTCAGACGGTTCAACGAATTGATGAAGTCGCATAGGCGAAAAGATGCATCGAAGAGGAAGACTACAGTTGGTGCTGAGGTCGAGGCTGAAGTCTCTAGCTTGGTAACAGACTTAAGTGGGAGTGTCAAGTCATCGAAGAAGAATTCTTTCTTTGGGAGTCGTTGGCCACGATCGAAGAAGGAAGCTTTGGGAGGTACCACTTAG

Protein sequence

MGIGEQSSFEGIMRFDGANFGYWKMQIKDYLTCKKVHKALKERPAGMKLEDWEDMDEQAVAIIRLCLSMNVASLVANETTAIASLKITFSDEVNAILLLTSLPESWETMKTAVSNSLGDKSLKFTEICDAATAEEIRRKESGKESTSSSASGSALAVQRGKEKVEYDDRQHRNSRKNGNGEIECYYCHKKGHYKRHCRKLKEDLKKLDANANAVVHDALVCVESNTMSVDQSPEWIVDSVASVHISSDRRLFTSFTAENYGMVRMGNGRLSKTRGIGNISLKTDSGTELVLRDVRYVPSIRMNLISAGKLDDDGYRSEFAGNMWKLTRGSELVVVGHRTSTVYTVRFDVAKGSERQRMHRAANGLDGDGIKPTALTTKIDPSVHVQQSGGKDKEKKKSLVGCEVIAPVVRRFNELMKSHRRKDASKRKTTVGAEVEAEVSSLVTDLSGSVKSSKKNSFFGSRWPRSKKEALGGTT
Homology
BLAST of Moc05g09520 vs. NCBI nr
Match: XP_034902342.1 (LOW QUALITY PROTEIN: uncharacterized protein LOC118039689 [Populus alba])

HSP 1 Score: 234.2 bits (596), Expect = 2.3e-57
Identity = 146/377 (38.73%), Postives = 215/377 (57.03%), Query Frame = 0

Query: 11  GIMRFDGANFGYWKMQIKDYLTCKKVH-KALKERPAGMKLEDWEDMDEQAVAIIRLCLSM 70
           GI +FDG +FGYWKMQI+DYL  KK+H   L  +P  M+ E+W+ +D Q + II+L LS 
Sbjct: 9   GIEKFDGTDFGYWKMQIEDYLYGKKLHLPLLGSKPEKMEAEEWQLLDRQVLGIIQLSLSR 68

Query: 71  NVASLVANE-----------------------TTAIASLKITFSDEVNAILLLTSLPESW 130
            VA  V  E                       T  ++S++I F DE+ A++LL SLP SW
Sbjct: 69  RVAHNVTKEKSTAKLMEALSGMYEKLSANNTITNQLSSVEIEFDDEIRALILLASLPSSW 128

Query: 131 ETMKTAVSNSLGDKSLKFTEICDAATAEEIRRKESGKESTSSSASGSALAV--------- 190
           E M+TAVSNS G   LK+ +I D   AEE+RRK+SG+    SS+SGSAL +         
Sbjct: 129 EGMRTAVSNSAGKSKLKYDDIRDLILAEEVRRKDSGE----SSSSGSALNINTRGRRQDR 188

Query: 191 --QRGKEKVEYDDRQHRNSRKNGNGEIECYYCHKKGHYKRHCRKLKEDLKKLDANANAV- 250
              RG+ K  Y  +    SRK    ++EC+ C K GH+ R+C K K+   K D+    + 
Sbjct: 189 GSSRGRSKSRYRSKSKFGSRK----QVECWNCGKPGHFSRNCTKPKK--PKADSTNATID 248

Query: 251 -VHDALVCVESNTMSVDQSPEWIVDSVASVHISSDRRLFTSFTAENYGMVRMGNGRLSKT 310
            V DA++ V  +     Q  EWI+DS AS H +    +  ++   ++G+V + +G     
Sbjct: 249 EVQDAIILVVQS-----QIDEWILDSGASFHCTPHNEMLQNYVGGDHGVVYLADGTPLNI 308

Query: 311 RGIGNISLKTDSGTELVLRDVRYVPSIRMNLISAGKLDDDGYRSEFAGNMWKLTRGSELV 350
            GIG++ +KT +G+   L++VR+VP ++  LIS G+LDD G+   FAG MWK+++G+ ++
Sbjct: 309 VGIGDVQIKTMNGSIWTLQNVRHVPELKKKLISVGQLDDSGHSILFAGGMWKVSKGAMVL 368

BLAST of Moc05g09520 vs. NCBI nr
Match: TKS15174.1 (hypothetical protein D5086_0000036030 [Populus alba])

HSP 1 Score: 232.3 bits (591), Expect = 8.9e-57
Identity = 142/372 (38.17%), Postives = 212/372 (56.99%), Query Frame = 0

Query: 11  GIMRFDGANFGYWKMQIKDYLTCKKVH-KALKERPAGMKLEDWEDMDEQAVAIIRLCLSM 70
           GI +FDG +FGYWKMQI+DYL  KK+H   L  +P  M+ E+W+ +D Q + II+L LS 
Sbjct: 9   GIEKFDGTDFGYWKMQIEDYLYGKKLHLPLLGSKPEKMEAEEWQLLDRQVLGIIQLSLSR 68

Query: 71  NVASLVANE-----------------------TTAIASLKITFSDEVNAILLLTSLPESW 130
            VA  V  E                       T  ++S++I F DE+ A++LL SLP SW
Sbjct: 69  RVAHNVTKEKSTAKLMEALSGMYEKLSANNTITNQLSSVEIEFDDEIRALILLASLPSSW 128

Query: 131 ETMKTAVSNSLGDKSLKFTEICDAATAEEIRRKESGKESTSSSASGSALAV--------- 190
           E M+TAVSNS G   LK+ +I D   AEE+RRK+SG+    SS+SGSAL +         
Sbjct: 129 EGMRTAVSNSAGKSKLKYDDIRDLILAEEVRRKDSGE----SSSSGSALNINTRGRRQDR 188

Query: 191 --QRGKEKVEYDDRQHRNSRKNGNGEIECYYCHKKGHYKRHCRKLKEDLKKLDANANAV- 250
              RG+ K  Y  +    SRK    ++EC+ C K GH+ R+C K K+   K D+    + 
Sbjct: 189 GSSRGRSKSRYRSKSKFGSRK----QVECWNCGKPGHFSRNCTKPKK--PKADSTNATID 248

Query: 251 -VHDALVCVESNTMSVDQSPEWIVDSVASVHISSDRRLFTSFTAENYGMVRMGNGRLSKT 310
            V DA++ V  +     Q  EWI+DS AS H +    +  ++   ++G+V + +G     
Sbjct: 249 EVQDAIILVVQS-----QIDEWILDSGASFHCTPHNEMLQNYVGGDHGVVYLADGTPLNI 308

Query: 311 RGIGNISLKTDSGTELVLRDVRYVPSIRMNLISAGKLDDDGYRSEFAGNMWKLTRGSELV 346
            GIG++ +KT +G+   L++VR+VP ++  LIS G+LDD G+   FAG MWK+++G+ ++
Sbjct: 309 VGIGDVQIKTMNGSIWTLQNVRHVPELKKKLISVGQLDDSGHSILFAGGMWKVSKGAMVL 365

BLAST of Moc05g09520 vs. NCBI nr
Match: KAG5549868.1 (hypothetical protein RHGRI_014986 [Rhododendron griersonianum])

HSP 1 Score: 231.1 bits (588), Expect = 2.0e-56
Identity = 148/407 (36.36%), Postives = 215/407 (52.83%), Query Frame = 0

Query: 12  IMRFDGANFGYWKMQIKDYLTCKKVHKALK---ERPAGMKLEDWEDMDEQAVAIIRLCLS 71
           ++ F+G N+  WK++++D L CK +H  ++    +P  MK EDW  ++ +AV +IR  + 
Sbjct: 8   MISFNGTNWVTWKIKMEDLLYCKDLHGPVEGDTAKPIDMKREDWTILNRKAVGLIRQWID 67

Query: 72  MNVASLVANETTA----------------------------------------------- 131
            +V   V+ ET+A                                               
Sbjct: 68  DSVFHHVSTETSAYDLWKKLESLYDRKSATNKAFLFKKLVNLKFKEGKSIAEHLNEMNSI 127

Query: 132 ---IASLKITFSDEVNAILLLTSLPESWETMKTAVSNSLGDKSLKFTEICDAATAEEIRR 191
              +AS+KI F DE+ A++LL+SLPE+WET+   VSNS  D  +  +++  +   EE RR
Sbjct: 128 VNQLASMKIVFDDELQALMLLSSLPETWETLVVTVSNSAPDGVVSKSQVTSSLLNEETRR 187

Query: 192 KESGKESTSSSASGSALAVQRGKEKVEYDDRQHRNS---RKNGNGEIECYYCHKKGHYKR 251
           K +G  S S +   +     RG+    ++  Q R S   R     +IEC+YC KKGH KR
Sbjct: 188 KSTG-SSHSEALVVNPRGRPRGRSSTPHNRDQSRGSSRGRSPSKQDIECHYCKKKGHMKR 247

Query: 252 HCRKLK-------EDLKKLDANANAVVHD---ALVCVESNTMSVDQSPEWIVDSVASVHI 311
            C KLK       +   K   N  AV  D    +VC ES    V Q   W++DS AS H+
Sbjct: 248 ECLKLKFKEENRAKHGDKKQENTTAVSSDGELVVVCDESCVNLVSQDTNWVIDSGASFHV 307

Query: 312 SSDRRLFTSFTAENYGMVRMGNGRLSKTRGIGNISLKTDSGTELVLRDVRYVPSIRMNLI 353
           +S    FTS+   ++G VRMGN  LSK  G+GNI L+T  G +LVL+DVR+VP IR+NLI
Sbjct: 308 TSRADFFTSYNEGDFGCVRMGNEGLSKIVGMGNICLETSVGCKLVLKDVRHVPDIRLNLI 367

BLAST of Moc05g09520 vs. NCBI nr
Match: RVX08463.1 (Retrovirus-related Pol polyprotein from transposon TNT 1-94 [Vitis vinifera])

HSP 1 Score: 230.7 bits (587), Expect = 2.6e-56
Identity = 137/356 (38.48%), Postives = 208/356 (58.43%), Query Frame = 0

Query: 5   EQSSFEGIMRFDGANFGYWKMQIKDYLTCKKVH-KALKERPAGMKLEDWEDMDEQAVAII 64
           E     GI +FDG +F YW+MQI+DYL  +K+H   L  +P  MK E+W   D Q + +I
Sbjct: 4   EAGKASGIEKFDGTDFAYWRMQIEDYLYGRKLHLPLLGTKPESMKAEEWVLFDRQVLGVI 63

Query: 65  RLCLSMNVASLVANETTAIASLK----ITFSDEVNAILLLTSLPESWETMKTAVSNSLGD 124
           RL LS +VA  V  E T    +K    I F DE+ A+++L SLP SWE M+ AVSNS G 
Sbjct: 64  RLTLSRSVAHNVVKEKTTADLMKALSEIDFDDEIRALIVLASLPNSWEAMRMAVSNSTGK 123

Query: 125 KSLKFTEICDAATAEEIRRKESGKESTSSSASGSALAVQ---RGKEKVEYDDRQH-RNSR 184
           + LK+ +I D   AEEIRR+++G+    +S SGSAL ++   RG  +     R + RNS 
Sbjct: 124 EKLKYNDIRDLILAEEIRRRDAGE----TSGSGSALNLETRGRGNNRNSNQGRSNSRNSN 183

Query: 185 KN-----GNGEIECYYCHKKGHYKRHCRKLKEDLKKLDANANAV---VHDALVCVESNTM 244
           +N        +++C+ C K GH+KR C+  K+  K  D +ANAV   V DAL+    + +
Sbjct: 184 RNISKSRSGQQVQCWNCGKTGHFKRQCKSPKK--KNEDDSANAVTEEVQDALLLAVDSPL 243

Query: 245 SVDQSPEWIVDSVASVHISSDRRLFTSFTAENYGMVRMGNGRLSKTRGIGNISLKTDSGT 304
                 +W++DS AS H +  R +  ++ A ++G V + +G      G+G++ +   +G 
Sbjct: 244 D-----DWVLDSGASFHTTPHREIIQNYVAGDFGKVYLADGSALDVVGLGDVRISLPNGY 303

Query: 305 ELVLRDVRYVPSIRMNLISAGKLDDDGYRSEFAGNMWKLTRGSELVVVGHRTSTVY 344
             +L  VR++P +R NLIS G+LDD+GY   F G  WK+T+G+ ++  G +T+T+Y
Sbjct: 304 VWLLEKVRHIPDLRRNLISVGQLDDEGYAILFVGGTWKVTKGARVLARGKKTNTLY 348

BLAST of Moc05g09520 vs. NCBI nr
Match: CAN65867.1 (hypothetical protein VITISV_034935 [Vitis vinifera])

HSP 1 Score: 230.7 bits (587), Expect = 2.6e-56
Identity = 136/356 (38.20%), Postives = 209/356 (58.71%), Query Frame = 0

Query: 5   EQSSFEGIMRFDGANFGYWKMQIKDYLTCKKVH-KALKERPAGMKLEDWEDMDEQAVAII 64
           E     GI +FDG +F YW+MQI+DYL  +K+H   L  +P  MK E+W  +D Q + +I
Sbjct: 4   EAGKASGIEKFDGTDFAYWRMQIEDYLYGRKLHLPLLGTKPESMKAEEWALLDRQVLGVI 63

Query: 65  RLCLSMNVASLVANETTAIASLK----ITFSDEVNAILLLTSLPESWETMKTAVSNSLGD 124
           RL LS +VA  V  E T    +K    I F DE+ A+++L SLP SWE M+ AVSNS G 
Sbjct: 64  RLTLSRSVAHNVVKEKTTADLMKALSEIDFDDEIRALIVLASLPNSWEAMRMAVSNSTGK 123

Query: 125 KSLKFTEICDAATAEEIRRKESGKESTSSSASGSALAVQ---RGKEKVEYDDRQH-RNSR 184
           + LK+ +I D   AEEIRR+++G+    +S SGSAL ++   RG  +     R + RNS 
Sbjct: 124 EKLKYNDIRDLILAEEIRRRDAGE----TSGSGSALNLETRGRGNNRNSNQGRSNSRNSN 183

Query: 185 KN-----GNGEIECYYCHKKGHYKRHCRKLKEDLKKLDANANAV---VHDALVCVESNTM 244
           +N        +++C+ C K GH+KR C+  K+  K  D +ANAV   V DAL+    + +
Sbjct: 184 RNRSKSRSGQQVQCWNCGKTGHFKRQCKSPKK--KNEDDSANAVTEEVQDALLLAVDSPL 243

Query: 245 SVDQSPEWIVDSVASVHISSDRRLFTSFTAENYGMVRMGNGRLSKTRGIGNISLKTDSGT 304
                 +W++DS AS H +  R +  ++ A ++G V + +G      G+G++ +   +G+
Sbjct: 244 D-----DWVLDSGASFHTTPHREIIQNYVAGDFGKVYLADGSALDVVGLGDVRISLPNGS 303

Query: 305 ELVLRDVRYVPSIRMNLISAGKLDDDGYRSEFAGNMWKLTRGSELVVVGHRTSTVY 344
             +L  VR++P +R NLIS G+LDD+G+   F G  WK+T+G+ ++  G +T T+Y
Sbjct: 304 VWLLEKVRHIPDLRRNLISVGQLDDEGHAILFVGGTWKVTKGARVLARGKKTGTLY 348

BLAST of Moc05g09520 vs. ExPASy Swiss-Prot
Match: P10978 (Retrovirus-related Pol polyprotein from transposon TNT 1-94 OS=Nicotiana tabacum OX=4097 PE=2 SV=1)

HSP 1 Score: 172.6 bits (436), Expect = 1.1e-41
Identity = 124/406 (30.54%), Postives = 196/406 (48.28%), Query Frame = 0

Query: 12  IMRFDGAN-FGYWKMQIKDYLTCKKVHKAL---KERPAGMKLEDWEDMDEQAVAIIRLCL 71
           + +F+G N F  W+ +++D L  + +HK L    ++P  MK EDW D+DE+A + IRL L
Sbjct: 8   VAKFNGDNGFSTWQRRMRDLLIQQGLHKVLDVDSKKPDTMKAEDWADLDERAASAIRLHL 67

Query: 72  SMNVASLVANETTA---------------------------------------------- 131
           S +V + + +E TA                                              
Sbjct: 68  SDDVVNNIIDEDTARGIWTRLESLYMSKTLTNKLYLKKQLYALHMSEGTNFLSHLNVFNG 127

Query: 132 ----IASLKITFSDEVNAILLLTSLPESWETMKTAVSNSLGDKSLKFTEICDAATAEEIR 191
               +A+L +   +E  AILLL SLP S++ + T + +  G  +++  ++  A    E  
Sbjct: 128 LITQLANLGVKIEEEDKAILLLNSLPSSYDNLATTILH--GKTTIELKDVTSALLLNEKM 187

Query: 192 RKESGKESTSSSASGSALAVQRGKEKVEYDDRQHRNSRKNGNGEIECYYCHKKGHYKRHC 251
           RK+   +  +    G   + QR          + ++  ++ +    CY C++ GH+KR C
Sbjct: 188 RKKPENQGQALITEGRGRSYQRSSNNYGRSGARGKSKNRSKSRVRNCYNCNQPGHFKRDC 247

Query: 252 ---RKLKEDL--KKLDANANAVVHD-----ALVCVESNTMSVD-QSPEWIVDSVASVHIS 311
              RK K +   +K D N  A+V +       +  E   M +     EW+VD+ AS H +
Sbjct: 248 PNPRKGKGETSGQKNDDNTAAMVQNNDNVVLFINEEEECMHLSGPESEWVVDTAASHHAT 307

Query: 312 SDRRLFTSFTAENYGMVRMGNGRLSKTRGIGNISLKTDSGTELVLRDVRYVPSIRMNLIS 353
             R LF  + A ++G V+MGN   SK  GIG+I +KT+ G  LVL+DVR+VP +RMNLIS
Sbjct: 308 PVRDLFCRYVAGDFGTVKMGNTSYSKIAGIGDICIKTNVGCTLVLKDVRHVPDLRMNLIS 367

BLAST of Moc05g09520 vs. ExPASy TrEMBL
Match: A0A4U5QVL9 (CCHC-type domain-containing protein OS=Populus alba OX=43335 GN=D5086_0000036030 PE=4 SV=1)

HSP 1 Score: 232.3 bits (591), Expect = 4.3e-57
Identity = 142/372 (38.17%), Postives = 212/372 (56.99%), Query Frame = 0

Query: 11  GIMRFDGANFGYWKMQIKDYLTCKKVH-KALKERPAGMKLEDWEDMDEQAVAIIRLCLSM 70
           GI +FDG +FGYWKMQI+DYL  KK+H   L  +P  M+ E+W+ +D Q + II+L LS 
Sbjct: 9   GIEKFDGTDFGYWKMQIEDYLYGKKLHLPLLGSKPEKMEAEEWQLLDRQVLGIIQLSLSR 68

Query: 71  NVASLVANE-----------------------TTAIASLKITFSDEVNAILLLTSLPESW 130
            VA  V  E                       T  ++S++I F DE+ A++LL SLP SW
Sbjct: 69  RVAHNVTKEKSTAKLMEALSGMYEKLSANNTITNQLSSVEIEFDDEIRALILLASLPSSW 128

Query: 131 ETMKTAVSNSLGDKSLKFTEICDAATAEEIRRKESGKESTSSSASGSALAV--------- 190
           E M+TAVSNS G   LK+ +I D   AEE+RRK+SG+    SS+SGSAL +         
Sbjct: 129 EGMRTAVSNSAGKSKLKYDDIRDLILAEEVRRKDSGE----SSSSGSALNINTRGRRQDR 188

Query: 191 --QRGKEKVEYDDRQHRNSRKNGNGEIECYYCHKKGHYKRHCRKLKEDLKKLDANANAV- 250
              RG+ K  Y  +    SRK    ++EC+ C K GH+ R+C K K+   K D+    + 
Sbjct: 189 GSSRGRSKSRYRSKSKFGSRK----QVECWNCGKPGHFSRNCTKPKK--PKADSTNATID 248

Query: 251 -VHDALVCVESNTMSVDQSPEWIVDSVASVHISSDRRLFTSFTAENYGMVRMGNGRLSKT 310
            V DA++ V  +     Q  EWI+DS AS H +    +  ++   ++G+V + +G     
Sbjct: 249 EVQDAIILVVQS-----QIDEWILDSGASFHCTPHNEMLQNYVGGDHGVVYLADGTPLNI 308

Query: 311 RGIGNISLKTDSGTELVLRDVRYVPSIRMNLISAGKLDDDGYRSEFAGNMWKLTRGSELV 346
            GIG++ +KT +G+   L++VR+VP ++  LIS G+LDD G+   FAG MWK+++G+ ++
Sbjct: 309 VGIGDVQIKTMNGSIWTLQNVRHVPELKKKLISVGQLDDSGHSILFAGGMWKVSKGAMVL 365

BLAST of Moc05g09520 vs. ExPASy TrEMBL
Match: A5BPB3 (Uncharacterized protein OS=Vitis vinifera OX=29760 GN=VITISV_034935 PE=4 SV=1)

HSP 1 Score: 230.7 bits (587), Expect = 1.3e-56
Identity = 136/356 (38.20%), Postives = 209/356 (58.71%), Query Frame = 0

Query: 5   EQSSFEGIMRFDGANFGYWKMQIKDYLTCKKVH-KALKERPAGMKLEDWEDMDEQAVAII 64
           E     GI +FDG +F YW+MQI+DYL  +K+H   L  +P  MK E+W  +D Q + +I
Sbjct: 4   EAGKASGIEKFDGTDFAYWRMQIEDYLYGRKLHLPLLGTKPESMKAEEWALLDRQVLGVI 63

Query: 65  RLCLSMNVASLVANETTAIASLK----ITFSDEVNAILLLTSLPESWETMKTAVSNSLGD 124
           RL LS +VA  V  E T    +K    I F DE+ A+++L SLP SWE M+ AVSNS G 
Sbjct: 64  RLTLSRSVAHNVVKEKTTADLMKALSEIDFDDEIRALIVLASLPNSWEAMRMAVSNSTGK 123

Query: 125 KSLKFTEICDAATAEEIRRKESGKESTSSSASGSALAVQ---RGKEKVEYDDRQH-RNSR 184
           + LK+ +I D   AEEIRR+++G+    +S SGSAL ++   RG  +     R + RNS 
Sbjct: 124 EKLKYNDIRDLILAEEIRRRDAGE----TSGSGSALNLETRGRGNNRNSNQGRSNSRNSN 183

Query: 185 KN-----GNGEIECYYCHKKGHYKRHCRKLKEDLKKLDANANAV---VHDALVCVESNTM 244
           +N        +++C+ C K GH+KR C+  K+  K  D +ANAV   V DAL+    + +
Sbjct: 184 RNRSKSRSGQQVQCWNCGKTGHFKRQCKSPKK--KNEDDSANAVTEEVQDALLLAVDSPL 243

Query: 245 SVDQSPEWIVDSVASVHISSDRRLFTSFTAENYGMVRMGNGRLSKTRGIGNISLKTDSGT 304
                 +W++DS AS H +  R +  ++ A ++G V + +G      G+G++ +   +G+
Sbjct: 244 D-----DWVLDSGASFHTTPHREIIQNYVAGDFGKVYLADGSALDVVGLGDVRISLPNGS 303

Query: 305 ELVLRDVRYVPSIRMNLISAGKLDDDGYRSEFAGNMWKLTRGSELVVVGHRTSTVY 344
             +L  VR++P +R NLIS G+LDD+G+   F G  WK+T+G+ ++  G +T T+Y
Sbjct: 304 VWLLEKVRHIPDLRRNLISVGQLDDEGHAILFVGGTWKVTKGARVLARGKKTGTLY 348

BLAST of Moc05g09520 vs. ExPASy TrEMBL
Match: A0A438JHN2 (Retrovirus-related Pol polyprotein from transposon TNT 1-94 OS=Vitis vinifera OX=29760 GN=POLX_3131 PE=4 SV=1)

HSP 1 Score: 230.7 bits (587), Expect = 1.3e-56
Identity = 137/356 (38.48%), Postives = 208/356 (58.43%), Query Frame = 0

Query: 5   EQSSFEGIMRFDGANFGYWKMQIKDYLTCKKVH-KALKERPAGMKLEDWEDMDEQAVAII 64
           E     GI +FDG +F YW+MQI+DYL  +K+H   L  +P  MK E+W   D Q + +I
Sbjct: 4   EAGKASGIEKFDGTDFAYWRMQIEDYLYGRKLHLPLLGTKPESMKAEEWVLFDRQVLGVI 63

Query: 65  RLCLSMNVASLVANETTAIASLK----ITFSDEVNAILLLTSLPESWETMKTAVSNSLGD 124
           RL LS +VA  V  E T    +K    I F DE+ A+++L SLP SWE M+ AVSNS G 
Sbjct: 64  RLTLSRSVAHNVVKEKTTADLMKALSEIDFDDEIRALIVLASLPNSWEAMRMAVSNSTGK 123

Query: 125 KSLKFTEICDAATAEEIRRKESGKESTSSSASGSALAVQ---RGKEKVEYDDRQH-RNSR 184
           + LK+ +I D   AEEIRR+++G+    +S SGSAL ++   RG  +     R + RNS 
Sbjct: 124 EKLKYNDIRDLILAEEIRRRDAGE----TSGSGSALNLETRGRGNNRNSNQGRSNSRNSN 183

Query: 185 KN-----GNGEIECYYCHKKGHYKRHCRKLKEDLKKLDANANAV---VHDALVCVESNTM 244
           +N        +++C+ C K GH+KR C+  K+  K  D +ANAV   V DAL+    + +
Sbjct: 184 RNISKSRSGQQVQCWNCGKTGHFKRQCKSPKK--KNEDDSANAVTEEVQDALLLAVDSPL 243

Query: 245 SVDQSPEWIVDSVASVHISSDRRLFTSFTAENYGMVRMGNGRLSKTRGIGNISLKTDSGT 304
                 +W++DS AS H +  R +  ++ A ++G V + +G      G+G++ +   +G 
Sbjct: 244 D-----DWVLDSGASFHTTPHREIIQNYVAGDFGKVYLADGSALDVVGLGDVRISLPNGY 303

Query: 305 ELVLRDVRYVPSIRMNLISAGKLDDDGYRSEFAGNMWKLTRGSELVVVGHRTSTVY 344
             +L  VR++P +R NLIS G+LDD+GY   F G  WK+T+G+ ++  G +T+T+Y
Sbjct: 304 VWLLEKVRHIPDLRRNLISVGQLDDEGYAILFVGGTWKVTKGARVLARGKKTNTLY 348

BLAST of Moc05g09520 vs. ExPASy TrEMBL
Match: A0A438IBT7 (Retrovirus-related Pol polyprotein from transposon TNT 1-94 OS=Vitis vinifera OX=29760 GN=POLX_788 PE=4 SV=1)

HSP 1 Score: 229.9 bits (585), Expect = 2.1e-56
Identity = 142/364 (39.01%), Postives = 213/364 (58.52%), Query Frame = 0

Query: 27  IKDYLTCKKVHKALK---ERPAGMKLEDWEDMDEQAVAIIRLCLSMNVASL--------- 86
           ++D L CK ++  ++    +P GMK ++W+ +D +AV  IR   ++N A L         
Sbjct: 1   MEDLLFCKDLNGPIEGDSGKPEGMKDDEWKKLDRKAVGFIRK-TAVNKAFLFRKLVNSKY 60

Query: 87  ------------VANETTAIASLKITFSDEVNAILLLTSLPESWETMKTAVSNSLGDKSL 146
                       + +    +A++KITF DE+ A+LLL+SLPESWET+   VSNS  D  +
Sbjct: 61  KEGTPIAEHLNEIKSIVNQLAAMKITFDDELQALLLLSSLPESWETLVVTVSNSTPDGVV 120

Query: 147 KFTEICDAATAEEIRRKESGKESTSSSASGSALAV---QRGKEKVEYD---DRQHRNSRK 206
             +++  +   EE RRK SG      S+   AL +   +RGK K +     D+    S  
Sbjct: 121 TMSQVTSSLLNEETRRKSSG------SSHSEALVIENRERGKSKSKASHNRDKSRGRSSS 180

Query: 207 NGNGEIECYYCHKKGHYKRHCRKL------KEDLKKLDANANAVVHDALVCVESNTMSVD 266
               ++ECYYCHKKGH KR CRKL      KE   +   N  AVV D  + +  + +SV+
Sbjct: 181 ISKKDVECYYCHKKGHMKRECRKLKFKEQNKEKNSEKKQNDTAVVTDGELMIIRDDVSVN 240

Query: 267 ---QSPEWIVDSVASVHISSDRRLFTSFTAENYGMVRMGNGRLSKTRGIGNISLKTDSGT 326
              Q  +W++DS AS H++S    FTS++  ++G VRMGN  +SK  G+G+I L+T++G 
Sbjct: 241 LIGQETDWVIDSGASFHVTSRADFFTSYSQGDFGNVRMGNEDVSKIFGMGDICLETNTGC 300

Query: 327 ELVLRDVRYVPSIRMNLISAGKLDDDGYRSEFAGNMWKLTRGSELVVVGHRTSTVYTVRF 352
           +L+LRDVR+VP IR+NLISAGKLDD+GY + F+   WKL++GS +V  G +T ++YTV+ 
Sbjct: 301 KLLLRDVRHVPDIRLNLISAGKLDDEGYNNNFSDGKWKLSKGSLVVAKGKKTCSLYTVQA 357

BLAST of Moc05g09520 vs. ExPASy TrEMBL
Match: A0A438HTJ3 (Retrovirus-related Pol polyprotein from transposon TNT 1-94 OS=Vitis vinifera OX=29760 GN=POLX_3129 PE=4 SV=1)

HSP 1 Score: 226.5 bits (576), Expect = 2.4e-55
Identity = 136/374 (36.36%), Postives = 212/374 (56.68%), Query Frame = 0

Query: 5   EQSSFEGIMRFDGANFGYWKMQIKDYLTCKKVH-KALKERPAGMKLEDWEDMDEQAVAII 64
           E     GI +FDG +F YW+MQI+DYL  +K+H   L  +P  MK E+W  +D Q + +I
Sbjct: 4   EAGKASGIEKFDGTDFAYWRMQIEDYLYGRKLHLPLLGTKPESMKAEEWALLDRQVLGVI 63

Query: 65  RLCLSMNVASLVANE----------------------TTAIASLKITFSDEVNAILLLTS 124
           RL LS +VA  V  E                      T  ++S++I F DE+ A+++L S
Sbjct: 64  RLTLSRSVAHNVVKEKTTADLMKALSVAQHLNEFNTITNQLSSVEIDFDDEIRALIVLAS 123

Query: 125 LPESWETMKTAVSNSLGDKSLKFTEICDAATAEEIRRKESGKESTSSSASGSALAVQ--- 184
           LP SWE M+ AVSNS G + LK+ +I D   AEEIRR+++G+    +S SGSAL ++   
Sbjct: 124 LPNSWEAMRMAVSNSTGKEKLKYNDIRDLILAEEIRRRDAGE----TSGSGSALNLETRG 183

Query: 185 RGKEKVEYDDRQH-RNSRKN-----GNGEIECYYCHKKGHYKRHCRKLKEDLKKLDANAN 244
           RG  +     R + RNS +N        +++C+ C K GH+KR C+  K+  K  D +AN
Sbjct: 184 RGNNRNSNQGRSNSRNSNRNRSKSRSGQQVQCWNCGKTGHFKRQCKSPKK--KNEDDSAN 243

Query: 245 AV---VHDALVCVESNTMSVDQSPEWIVDSVASVHISSDRRLFTSFTAENYGMVRMGNGR 304
           AV   V DAL+    + +      +W++DS AS H +  R +  ++ A ++G V + +G 
Sbjct: 244 AVTEEVQDALLLAVDSPLD-----DWVLDSGASFHTTPHREIIQNYVAGDFGKVYLADGS 303

Query: 305 LSKTRGIGNISLKTDSGTELVLRDVRYVPSIRMNLISAGKLDDDGYRSEFAGNMWKLTRG 344
                G+G++ +   +G+  +L  VR++P +R NLIS G+LDD+G+   F G  WK+T+G
Sbjct: 304 ALDVVGLGDVRISLPNGSVWLLEKVRHIPDLRRNLISVGQLDDEGHAILFVGGTWKVTKG 363

BLAST of Moc05g09520 vs. TAIR 10
Match: AT3G29785.1 (unknown protein; Has 90 Blast hits to 90 proteins in 7 species: Archae - 0; Bacteria - 0; Metazoa - 0; Fungi - 0; Plants - 90; Viruses - 0; Other Eukaryotes - 0 (source: NCBI BLink). )

HSP 1 Score: 53.1 bits (126), Expect = 6.9e-07
Identity = 25/74 (33.78%), Postives = 45/74 (60.81%), Query Frame = 0

Query: 14 RFDGANFGYWKMQIKDYLTCKKVHKALKERPAGMKLEDWEDMDEQAVAIIRLCLSMNVAS 73
          + DG ++ + +M+I+DYL  KK+H+ L ++   M  +DW  +  Q + +IRL +S N+A 
Sbjct: 8  KVDGTSYSFCRMKIEDYLYGKKLHQPLGKKVETMSQDDWNILYRQVLDVIRLTISKNIAH 67

Query: 74 LVANETTAIASLKI 88
           VA E +    +K+
Sbjct: 68 NVAKEKSPDGLMKV 81

BLAST of Moc05g09520 vs. TAIR 10
Match: AT3G21000.1 (Gag-Pol-related retrotransposon family protein )

HSP 1 Score: 45.1 bits (105), Expect = 1.9e-04
Identity = 32/144 (22.22%), Postives = 61/144 (42.36%), Query Frame = 0

Query: 172 RNSRKNGNGEIECYYCHKKGHYKRHCRKLKEDLKKLDANANAVVHDALVCVESNTMSVDQ 231
           ++ R     E  C  C+K  H +  C K +    K +     VV   L  V +       
Sbjct: 218 KDLRLKSKSEKWCGLCYKNNHNQEDC-KFRIHTDKEEKEDEIVVDYRLETVPNLGAKTYD 277

Query: 232 SPEWIVDSVASVHISSDRRLFTSFTAENYGMVRMGNGRLSKTRGIGNISLKTDSGTELVL 291
              WI+  +A ++++   + FT+        V   +G +    G G++ ++   G +  +
Sbjct: 278 DDIWIIHKMAPINMTPYVKYFTTLDRTFKATVGTVDGTVLLVEGKGDVKIRMKEGKKKTI 337

Query: 292 RDVRYVPSIRMNLISAGKLDDDGY 316
           R+V +VP +  N++S GK+    Y
Sbjct: 338 RNVIFVPGLNRNVLSFGKMVSKRY 360

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_034902342.12.3e-5738.73LOW QUALITY PROTEIN: uncharacterized protein LOC118039689 [Populus alba][more]
TKS15174.18.9e-5738.17hypothetical protein D5086_0000036030 [Populus alba][more]
KAG5549868.12.0e-5636.36hypothetical protein RHGRI_014986 [Rhododendron griersonianum][more]
RVX08463.12.6e-5638.48Retrovirus-related Pol polyprotein from transposon TNT 1-94 [Vitis vinifera][more]
CAN65867.12.6e-5638.20hypothetical protein VITISV_034935 [Vitis vinifera][more]
Match NameE-valueIdentityDescription
P109781.1e-4130.54Retrovirus-related Pol polyprotein from transposon TNT 1-94 OS=Nicotiana tabacum... [more]
Match NameE-valueIdentityDescription
A0A4U5QVL94.3e-5738.17CCHC-type domain-containing protein OS=Populus alba OX=43335 GN=D5086_0000036030... [more]
A5BPB31.3e-5638.20Uncharacterized protein OS=Vitis vinifera OX=29760 GN=VITISV_034935 PE=4 SV=1[more]
A0A438JHN21.3e-5638.48Retrovirus-related Pol polyprotein from transposon TNT 1-94 OS=Vitis vinifera OX... [more]
A0A438IBT72.1e-5639.01Retrovirus-related Pol polyprotein from transposon TNT 1-94 OS=Vitis vinifera OX... [more]
A0A438HTJ32.4e-5536.36Retrovirus-related Pol polyprotein from transposon TNT 1-94 OS=Vitis vinifera OX... [more]
Match NameE-valueIdentityDescription
AT3G29785.16.9e-0733.78unknown protein; Has 90 Blast hits to 90 proteins in 7 species: Archae - 0; Bact... [more]
AT3G21000.11.9e-0422.22Gag-Pol-related retrotransposon family protein [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (OHB3-1) v2
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 197..217
NoneNo IPR availableGENE3D4.10.60.10coord: 156..209
e-value: 1.1E-6
score: 30.4
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 444..460
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 444..475
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 136..174
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 160..174
NoneNo IPR availablePANTHERPTHR34676:SF1ZINC FINGER, CCHC-TYPE, TUBBY C-TERMINAL-LIKE DOMAIN PROTEIN-RELATEDcoord: 11..82
coord: 83..346
NoneNo IPR availablePANTHERPTHR34676FAMILY NOT NAMEDcoord: 11..82
coord: 83..346
IPR001878Zinc finger, CCHC-typePROSITEPS50158ZF_CCHCcoord: 184..199
score: 9.257132
IPR036875Zinc finger, CCHC-type superfamilySUPERFAMILY57756Retrovirus zinc finger-like domainscoord: 166..206

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Moc05g09520.1Moc05g09520.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0003676 nucleic acid binding
molecular_function GO:0008270 zinc ion binding