Moc05g09240 (gene) Bitter gourd (OHB3-1) v2

Overview
NameMoc05g09240
Typegene
OrganismMomordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
DescriptionRNase H domain-containing protein
Locationchr5: 7172488 .. 7176100 (-)
RNA-Seq ExpressionMoc05g09240
SyntenyMoc05g09240
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGACAGAAACTATTCAGCAATTGAAAGGTTCAGAGACACTGTGGACGATTGTTGCTTGTAGGATCTTGGTTTCTTGGGGCAAAGATACACTTGGTGCAATAGAATTTAGAATAGTGACCAAATCAGCGAAAGGTTGGACCGCTTCCTGGGGAATGAAAAGTTTTGCTGTTTGTGGAGGGAGTCGTATGTAACCCATGAGGACTGGTCAAAATCGGACCATCGTCCAATTCTTCTAACTTTGGGAGGGATAACCAGAAAAATTCACAAACGGAGGTCTCTCTTCAGGTTTGAGGAAGTTTGGACAAAATCAAGAAGGTGTGAAGAAATTATTGGTGCAAATGGAGATTGAGACTCTTCCATTCCTTCTGCAAAGCTTGAATCTTGTTTAGCTAGCTGTGCAACTGCACTGGGGAAATGGGGCAGAGACTTTAATAGAGGTTTCAAAAGGCAAATAAAGAAAATCTGGTTGAAAATTGCCCATATATATAACGAACCTCCTCCTTGGGATTTTGCAGCCATTCATAGCTTGGATAGGGAGCTTGACGACCTTCTTGAATCTGATGAAATTTATTGGAAGCAGAGGTCTCGTGAAGACTGGTTGAAGTGGGGGGATAGAAATTCGAAATGGTTTCACAAGAAAGCTACAATAAGGAAAAAGAGGAATTCGATCAGAGGCATCGAGGTTGAGGGAGTCTGGACGGAGGAGCCACAGTTGATTGAGTATCAGTTTATTAGTTATTTTAAGGAGTTGTTTAACTTCAAATCCCTCACATGATGACATGGCTAAAGTGTTACAGTATATACCTTGCTCAGTAACGGGGGAGATGAACGAAAAGCTCATTGCTCCATTTACCAGGTCTGATATTGAACGAGATTTGAAGTAAATGTATCCCACAAAGGCGCCGGGTACGGATGGTTTTCCAGCTCTTTTCTACCAAAAATATTGGGGACTTATTGGAGATAAAATGGTGGGCTGTTTTCTAGAGGTGTTGAATAGGAGGAAATCGATTACCTTGATTCCAAAGGTGCGGGAGCCGGGAGTGGTTTCAGAGTTCCATCCTATAAGCCTCTGTAATGTCTTGTACAAAATTGTAATGAAGGTGTTAGCAAATTTTAAAGCTAGTTCTTGACAAAATTGTATCAGATTGCCAGGCAACCTTCATCTCTGGTAGGGTCATTAATGATAACATTATTATTGGTCATGAATGTATCCATGCCATGAAGAAGAGGAGGAAATGAAGGGAAGGTTTGGCGGCGCTAAAGCTTGATATGAGTAAGGCATACGATCGGGTGGAATGGGTCTTTTTAAAGCAAATGATGCTGAAGATGGGTTTCAATGAGGACTGGATTGAGATCATTATGGATTGCATTAGTACTTCATCCTTTGCCATCATGATTAATGGGGAAGCAAAAGAGCATATCATCCCTCATAGGGGCTTACACCAAGGGGATCCTTTGTCCCCTTATCTATTTGTGCAGAAGGCCTCTTAGCATCCATTAAACATGCCCATGAGAACAATTGAGTTTCAGGTATGTCGATTAACTCAAATTGTCCAAGTATCTCTCATCTTCTCTTTGCAGATGATAGCCTAGTATTTTGTAAGGCTAAAATATCAAAATTGTATGCGATTAAATTACTTTTGCTGGACTATGAGAAAGCTTCTGGACAAAAAATTAATTTTGATAAGTCCATGATTTGGTTATCTCCCAACGTGCATGATGATTTCCGTAATTACTTTCAATCGGTGGTGGGAATGAGGTTGGTCCCAAATTTGGGAGATTACCTTGGGCTTCCCTTCAAGTCTCTCTCGAAATAAACGTGGAGATTTTGATTTTATAAAGGAGAGAGTGTGGAGAGCTGTCCAAGGGTGAAAAGGAAAAATTTTCTCAGGCAGAGGAAAAGAGATCTTGATTAAGAGTGTCGCCTAAGCTATCCTGACTTATGTCATGAGTTGTTTTCGTCTCCCAAAGATGTTTTGTTCAGATATTTCGAGGGACTTGGCTAAGTTTTAGTGGGGTACAAATGATGCTAGAAGGAGAATCCATTGGATGAGTTGGGACCGTATGTGTTGGCCGAAGGAGTTAGGAGGTCTAAATTTTTGAGATCTCGTGATGTTTAACAAGATCTTATTGGCAAAACAATTGTAGAGGTTTCTTCAACTGCCTAATCTTCTTGTTTCCAGGGTTATTAGAGGGGTGTATGCATGTGAGGATTCTATTCTGACAGCAAAGGCGGACTACCACTCATCCTACTTTTGGCAAGGGATGGTTTGGTGTCGTGATTTGCTGCTTATGGGAAGTGGTTGGAGATGGGTCTTCAATTAATTTTTTCAAAGATCCGTGGATTCCGCGAGAGACCACTTTCAGGCCTTTAAGTCCTTGTCTTGATCTGTCCATAATTTTTTGGGTAGCTGATTTTGTTACTTCCTCTCGAAATTGGGACGTCCAAAAGCTCAGGTCAATAGTTGGAGAAGAAGATGTGGCTACCATATCTAATATTCCGATTAGTGCTGTTGGGGTTCAGATAGATGGATGTGGCATTTTACGAAGAATGACTCCTATACAGTAAGAGGTGGGTACAAGTTAGTTATGAGATTGAATCTACTTTCCCTCAGACAAAAATGAGAGGTGGTGGAAGGATTTTTGGAAAATGGACCTTCCCCCAAAAATTAAATCTTTCTTTGGAAAGCAATCATAAATTGTATCCCAGTAATGTCAAATTTGAGGGGAAGAGGTATGGAGGTGTTACATGTGTGTCCGATTTGCCTGAAAAAGGAGGAGACGGTGGATCACGCTCTTGTGGGTTGTAAAAGGGCGTCCAAAATCTGATCCTTGCTTCTGCCAGCATTAGATTCTTCCAATAATTTTAACAGTTGTTTTGTCGACCGGTGGACTAAGGTGATGTCAGATCTTTCGCAAAAAGAAGTCACCCTCGCCGGAATTACTAGCTGGGCCTTGTGGAAGGATCGAAATTCACACATTTTCAAAAAGGGTACACCTGATATTCATTTCAAATGAATATGGACTTTAGATTATGCTAATGCATTTCTGAATAAAAATAAATCGACCTTGGAGATGCAAGTGGCCCCCTCCTGCAATCCCGCCGTCTGGTGTCCCCCTCCAAAAGGGTTTTTAAAAATCAATACTGATGCAGCGTGTACCACCTCTGTTGGAAAGTCCGGAATAGGGATTTTAATCCGTGATGATGAAAATCGGGTACAAGCAGCGATGATTTCTACCCAGGGTTTGGGAAGCCCCCTAATGGCAGAAATCCTTGCTATCAGAGAAGGAATTCGTTCGGCGATCCGGATGGGTTTCAGTAAGGTGGTGGTCGAATCTGACTCCCTCGAAGCGGTTAAACTAATAGAGAATGATGAGGGGTGCTTAAATGAAGTGGCTTCATGGATTGTTGACATCAGACGCTTGTGTGCAAATTTTGAGGATTTTCGAATTCAGCATGTTCGTCGCAAGGGAAATATGGCTGCTCATATTCTGGCTAGAAATTCTATTTCTATAAGTAGACCCTTTCTTTGGCTTTATGATTTTCCTGTTTGGCTTCTTAGCCGTGTGAAAGCTGATGGTCTCACTCCTATGGCCTTTATGACAAGTTAA

mRNA sequence

ATGACAGAAACTATTCAGCAATTGAAAGGTTCAGAGACACTGTGGACGATTGTTGCTTGTAGGATCTTGGTTTCTTGGGGCAAAGATACACTTGGTGCAATAGAATTTAGAATAGTGACCAAATCAGCGAAAGGTTGGACCGCTTCCTGGGGAATGAAAAGTTTTGCTGTTTGTGGAGGGAGTCCTAGCTGTGCAACTGCACTGGGGAAATGGGGCAGAGACTTTAATAGAGGTTTCAAAAGGCAAATAAAGAAAATCTGGTTGAAAATTGCCCATATATATAACGAACCTCCTCCTTGGGATTTTGCAGCCATTCATAGCTTGGATAGGGAGCTTGACGACCTTCTTGAATCTGATGAAATTTATTGGAAGCAGAGGTCTCGTGAAGACTGGTTGAAGTGGGGGGATAGAAATTCGAAATGGTTTCACAAGAAAGCTACAATAAGGAAAAAGAGGAATTCGATCAGAGGCATCGAGGTTGAGGGAGTCTGGACGGAGGAGCCACAGTTGATTGATAACGGGGGAGATGAACGAAAAGCTCATTGCTCCATTTACCAGCAAAGGCGGACTACCACTCATCCTACTTTTGGCAAGGGATGGTTTGGTGTCGTGATTTGCTGCTTATGGGAAGTGGTTGGAGATGGGTCTTCAATTAATTTTTTCAAAGATCCGTGGATTCCGCGAGAGACCACTTTCAGGCCTTTAAGTCCTTGTCTTGATCTGTCCATAATTTTTTGGGTAGCTGATTTTGTTACTTCCTCTCGAAATTGGGACGTCCAAAAGCTCAGGTCAATAGTTGGAGAAGAAGATGTGGCTACCATATCTAATATTCCGATTAGTGCTGTTGGGGTTCAGATAGATGGATGTGGCATTTTACGAAGAATGACTCCTATACATAATGTCAAATTTGAGGGGAAGAGGTATGGAGGTGTTACATGTGTGTCCGATTTGCCTGAAAAAGGAGGAGACGGTGGATCACGCTCTTATTATGCTAATGCATTTCTGAATAAAAATAAATCGACCTTGGAGATGCAAGTGGCCCCCTCCTGCAATCCCGCCGTCTGGTGTCCCCCTCCAAAAGGGTTTTTAAAAATCAATACTGATGCAGCGTGTACCACCTCTGTTGGAAAGTCCGGAATAGGGATTTTAATCCGTGATGATGAAAATCGGGTACAAGCAGCGATGATTTCTACCCAGGGTTTGGGAAGCCCCCTAATGGCAGAAATCCTTGCTATCAGAGAAGGAATTCGTTCGGCGATCCGGATGGGTTTCAGTAAGGTGGTGGTCGAATCTGACTCCCTCGAAGCGGTTAAACTAATAGAGAATGATGAGGGGTGCTTAAATGAAGTGGCTTCATGGATTGTTGACATCAGACGCTTGTGTGCAAATTTTGAGGATTTTCGAATTCAGCATGTTCGTCGCAAGGGAAATATGGCTGCTCATATTCTGGCTAGAAATTCTATTTCTATAAGTAGACCCTTTCTTTGGCTTTATGATTTTCCTGTTTGGCTTCTTAGCCGTGTGAAAGCTGATGGTCTCACTCCTATGGCCTTTATGACAAGTTAA

Coding sequence (CDS)

ATGACAGAAACTATTCAGCAATTGAAAGGTTCAGAGACACTGTGGACGATTGTTGCTTGTAGGATCTTGGTTTCTTGGGGCAAAGATACACTTGGTGCAATAGAATTTAGAATAGTGACCAAATCAGCGAAAGGTTGGACCGCTTCCTGGGGAATGAAAAGTTTTGCTGTTTGTGGAGGGAGTCCTAGCTGTGCAACTGCACTGGGGAAATGGGGCAGAGACTTTAATAGAGGTTTCAAAAGGCAAATAAAGAAAATCTGGTTGAAAATTGCCCATATATATAACGAACCTCCTCCTTGGGATTTTGCAGCCATTCATAGCTTGGATAGGGAGCTTGACGACCTTCTTGAATCTGATGAAATTTATTGGAAGCAGAGGTCTCGTGAAGACTGGTTGAAGTGGGGGGATAGAAATTCGAAATGGTTTCACAAGAAAGCTACAATAAGGAAAAAGAGGAATTCGATCAGAGGCATCGAGGTTGAGGGAGTCTGGACGGAGGAGCCACAGTTGATTGATAACGGGGGAGATGAACGAAAAGCTCATTGCTCCATTTACCAGCAAAGGCGGACTACCACTCATCCTACTTTTGGCAAGGGATGGTTTGGTGTCGTGATTTGCTGCTTATGGGAAGTGGTTGGAGATGGGTCTTCAATTAATTTTTTCAAAGATCCGTGGATTCCGCGAGAGACCACTTTCAGGCCTTTAAGTCCTTGTCTTGATCTGTCCATAATTTTTTGGGTAGCTGATTTTGTTACTTCCTCTCGAAATTGGGACGTCCAAAAGCTCAGGTCAATAGTTGGAGAAGAAGATGTGGCTACCATATCTAATATTCCGATTAGTGCTGTTGGGGTTCAGATAGATGGATGTGGCATTTTACGAAGAATGACTCCTATACATAATGTCAAATTTGAGGGGAAGAGGTATGGAGGTGTTACATGTGTGTCCGATTTGCCTGAAAAAGGAGGAGACGGTGGATCACGCTCTTATTATGCTAATGCATTTCTGAATAAAAATAAATCGACCTTGGAGATGCAAGTGGCCCCCTCCTGCAATCCCGCCGTCTGGTGTCCCCCTCCAAAAGGGTTTTTAAAAATCAATACTGATGCAGCGTGTACCACCTCTGTTGGAAAGTCCGGAATAGGGATTTTAATCCGTGATGATGAAAATCGGGTACAAGCAGCGATGATTTCTACCCAGGGTTTGGGAAGCCCCCTAATGGCAGAAATCCTTGCTATCAGAGAAGGAATTCGTTCGGCGATCCGGATGGGTTTCAGTAAGGTGGTGGTCGAATCTGACTCCCTCGAAGCGGTTAAACTAATAGAGAATGATGAGGGGTGCTTAAATGAAGTGGCTTCATGGATTGTTGACATCAGACGCTTGTGTGCAAATTTTGAGGATTTTCGAATTCAGCATGTTCGTCGCAAGGGAAATATGGCTGCTCATATTCTGGCTAGAAATTCTATTTCTATAAGTAGACCCTTTCTTTGGCTTTATGATTTTCCTGTTTGGCTTCTTAGCCGTGTGAAAGCTGATGGTCTCACTCCTATGGCCTTTATGACAAGTTAA

Protein sequence

MTETIQQLKGSETLWTIVACRILVSWGKDTLGAIEFRIVTKSAKGWTASWGMKSFAVCGGSPSCATALGKWGRDFNRGFKRQIKKIWLKIAHIYNEPPPWDFAAIHSLDRELDDLLESDEIYWKQRSREDWLKWGDRNSKWFHKKATIRKKRNSIRGIEVEGVWTEEPQLIDNGGDERKAHCSIYQQRRTTTHPTFGKGWFGVVICCLWEVVGDGSSINFFKDPWIPRETTFRPLSPCLDLSIIFWVADFVTSSRNWDVQKLRSIVGEEDVATISNIPISAVGVQIDGCGILRRMTPIHNVKFEGKRYGGVTCVSDLPEKGGDGGSRSYYANAFLNKNKSTLEMQVAPSCNPAVWCPPPKGFLKINTDAACTTSVGKSGIGILIRDDENRVQAAMISTQGLGSPLMAEILAIREGIRSAIRMGFSKVVVESDSLEAVKLIENDEGCLNEVASWIVDIRRLCANFEDFRIQHVRRKGNMAAHILARNSISISRPFLWLYDFPVWLLSRVKADGLTPMAFMTS
Homology
BLAST of Moc05g09240 vs. NCBI nr
Match: XP_022158489.1 (uncharacterized protein LOC111024968 [Momordica charantia])

HSP 1 Score: 133.3 bits (334), Expect = 6.2e-27
Identity = 70/165 (42.42%), Postives = 99/165 (60.00%), Query Frame = 0

Query: 355 WCPPPKGFLKINTDAACTTSVGKSGIGILIRDDENRVQAAMISTQGLGSPLMAEILAIRE 414
           W PP  G +K+NTDAA   S   SG+G+L+R+    +  AM+    + +PL+A+ILAIRE
Sbjct: 67  WRPPLGGVVKMNTDAA--VSEEGSGVGVLLREGNAEIVGAMVDFHMVKTPLLAKILAIRE 126

Query: 415 GIRSAIRMGFSKVVVESDSLEAVKLIENDEGCLNEVASWIVDIRRLCANFEDFRIQHVRR 474
           G+  A R+G  +VVVE+DSLEA+ LI +      E  SW+ DIR    +F++   QHV R
Sbjct: 127 GLSLATRLGVHRVVVETDSLEALNLIGDKSPWKGEAVSWVEDIRAFARDFQEICFQHVFR 186

Query: 475 KGNMAAHILARNSISISRPFLWLYDFPVWLLSRVKADGLTPMAFM 520
           + N  A+ L R +IS+   FLW  DFP+WL    +A     +A M
Sbjct: 187 ESNAVAYHLGRETISLRCGFLWRLDFPIWLGRLAEAGKQNSVALM 229

BLAST of Moc05g09240 vs. NCBI nr
Match: KAF4371757.1 (hypothetical protein G4B88_030861 [Cannabis sativa])

HSP 1 Score: 119.4 bits (298), Expect = 9.3e-23
Identity = 75/214 (35.05%), Postives = 114/214 (53.27%), Query Frame = 0

Query: 64  CATALGKWGRDFNRGFKRQIKKIWLKIAHIYNEPPPW-DFAAIHSLDRELDDLLESDEIY 123
           C   LG+W +D      R++++   ++  + +   P      +  L+  L+DLL  +E Y
Sbjct: 666 CVERLGEWNKDKFGSIPRRVRETQKQLDDLLSVAAPLVRMDEVKRLETTLNDLLSREECY 725

Query: 124 WKQRSREDWLKWGDRNSKWFHKKATIRKKRNSIRGIEVEGVWTEEPQLIDNGGD-----E 183
           WK RSR DWL  GDRN+K+FH KAT RKK+N+I    VE + TE+ + +   GD     E
Sbjct: 726 WKLRSRADWLAMGDRNTKYFHNKATGRKKKNAI----VE-MMTEDGRKLTLEGDIVSEIE 785

Query: 184 R-------KAHCSIYQQRR--TTTHPTFGKGWFGVVICCLWEVVGDGSSINFFKDPWIPR 243
           R        A  S+ Q     T+    FG          + +VVGDG S++ F+DPW+PR
Sbjct: 786 RYFGTIFSSASPSVQQVENGSTSIEARFG----------MRKVVGDGQSVDAFRDPWLPR 845

Query: 244 ETTFRPLSPCLDLSIIFWVADFVTSSRNWDVQKL 263
             TFRP+SP  + S++  V++ +    +WDV+ L
Sbjct: 846 PRTFRPISPAPNASVM--VSELMGVDGSWDVRSL 862

BLAST of Moc05g09240 vs. NCBI nr
Match: KAF4370225.1 (hypothetical protein F8388_007366 [Cannabis sativa])

HSP 1 Score: 119.4 bits (298), Expect = 9.3e-23
Identity = 75/214 (35.05%), Postives = 114/214 (53.27%), Query Frame = 0

Query: 64  CATALGKWGRDFNRGFKRQIKKIWLKIAHIYNEPPPW-DFAAIHSLDRELDDLLESDEIY 123
           C   LG+W +D      R++++   ++  + +   P      +  L+  L+DLL  +E Y
Sbjct: 388 CVERLGEWNKDKFGSIPRRVRETQKQLDDLLSVAAPLVRMDEVKRLETTLNDLLSREECY 447

Query: 124 WKQRSREDWLKWGDRNSKWFHKKATIRKKRNSIRGIEVEGVWTEEPQLIDNGGD-----E 183
           WK RSR DWL  GDRN+K+FH KAT RKK+N+I    VE + TE+ + +   GD     E
Sbjct: 448 WKLRSRADWLAMGDRNTKYFHNKATGRKKKNAI----VE-MMTEDGRKLTLEGDIVSEIE 507

Query: 184 R-------KAHCSIYQQRR--TTTHPTFGKGWFGVVICCLWEVVGDGSSINFFKDPWIPR 243
           R        A  S+ Q     T+    FG          + +VVGDG S++ F+DPW+PR
Sbjct: 508 RYFGTIFSSASPSVQQVENGSTSIEARFG----------MRKVVGDGQSVDAFRDPWLPR 567

Query: 244 ETTFRPLSPCLDLSIIFWVADFVTSSRNWDVQKL 263
             TFRP+SP  + S++  V++ +    +WDV+ L
Sbjct: 568 PRTFRPISPAPNASVM--VSELMGVDGSWDVRSL 584

BLAST of Moc05g09240 vs. NCBI nr
Match: KAF2323497.1 (hypothetical protein GH714_035735 [Hevea brasiliensis])

HSP 1 Score: 118.6 bits (296), Expect = 1.6e-22
Identity = 116/526 (22.05%), Postives = 214/526 (40.68%), Query Frame = 0

Query: 64  CATALGKWGRDFNRGFKRQIKKIWLKIAHIYNEPPPWDFAAIHSLDRELDDLLESDEIYW 123
           C+  LG+WG +  + +K++I      +  +  +       +      +  DLL   E YW
Sbjct: 183 CSVRLGEWGDNLRKLYKQEIDDCKRVMGLLRGKRDAHSRESFRMAKSKFLDLLRRKESYW 242

Query: 124 KQRSREDWLKWGDRNSKWFHKKATIRKKRNSIRGIEVE-GVWTEEPQLIDNGGDERKAHC 183
           +QR++E WLK GD+N+K+FH+KATIR+++N I  ++ + G W +    ++       A  
Sbjct: 243 RQRAKEFWLKEGDQNTKFFHRKATIRQRKNRIDRLKDDNGNWCDWSTGLE-------AMI 302

Query: 184 SIYQQRRTTTHPTFGKGWFGVVICCLWEVVGDGSSINFFKDPWIPRETTFRPLSPCLDLS 243
           + Y +   T++     G    +I  +  +V D  +++  K PW+P +  F  ++   D +
Sbjct: 303 TDYFKNIFTSN----AGSSNDIIDLVPALVTDDQNVDLMK-PWLP-DVAFPMVTTTFDFN 362

Query: 244 I-IFWVADFVTSSRNWDVQKLRSIVGEEDVATISNIPI---SAVGVQ-----------ID 303
             ++ V+D +T    W++  +  +  E D   I +IP+   S++ +Q           + 
Sbjct: 363 FGVYMVSDLITDGA-WNIDLVNRVFNERDRKLILSIPLRRSSSIDLQFWKHDKRGSYSVS 422

Query: 304 GCGILRRM---TPIHNVKF-----------EGKRYGGVTCV--------------SDLPE 363
               L+     T + N KF           + ++     C+              SD  +
Sbjct: 423 SAYKLQTQESDTAVGNKKFWKKLWSIPAAPKNQKLSLACCIWFSTDQIKTLFSACSDHRD 482

Query: 364 ---------KGGDGGSRSYYANAFLNKNKSTLEMQVAPSCN------------------- 423
                       D  + +Y   +   +  S +     P+ N                   
Sbjct: 483 WLMRALALFNPNDSATLAYLCWSIWEERNSVVWKSAQPAANSCRLRGLRLKREWDDALVS 542

Query: 424 -------------PAVWCPPPKGFLKINTDAACTTSVGKSGIGILIRDDENRVQAAMIST 483
                        P  W  P   ++K+N D A   + G +GIG+++R++     A  IS 
Sbjct: 543 IRAAIPPTADLPTPQEWKKPGPNWVKLNVDIATNVNSGWTGIGMVVRNEMGSFVACKISR 602

Query: 484 Q-GLGSPLMAEILAIREGIRSAIRMGFSKVVVESDSLEAVKLIEN-DEGCLNEVASWIVD 502
             GL SP +AEI+ +RE +       +  V+VESD+L+ V ++ + +    + +   + D
Sbjct: 603 MVGLFSPTIAEIMGVREALSWIKDNNWQNVIVESDNLQVVNVLNSMNVENFSMMGGIVSD 662

BLAST of Moc05g09240 vs. NCBI nr
Match: XP_023897447.1 (uncharacterized protein LOC112009345 [Quercus suber])

HSP 1 Score: 118.6 bits (296), Expect = 1.6e-22
Identity = 99/381 (25.98%), Postives = 160/381 (41.99%), Query Frame = 0

Query: 185  YQQRRTTTHPTFGKG----WFGVVIC-------CLWEVVGDGSSINFFKDPWIPRETTFR 244
            Y   R   H T G      W  +++        C W  VG GS+I  + D W+PR  ++ 
Sbjct: 749  YFPSRDFLHATAGNNPSYTWRSILVAKSIIEKGCRWR-VGSGSNIKVWGDKWLPRAASYE 808

Query: 245  PLSPCLDLSIIFWVADFVTSSR-NWDVQKLRSIVGEEDVATISNIPIS-------AVGVQ 304
             +SP L L     V++F+   R  W  + +R I    D+  I  IP+S        +  +
Sbjct: 809  VISPRLFLHPETKVSEFICQERCCWKEEIIRQIFFPVDMEVILGIPLSTRFPEDRVIWAE 868

Query: 305  IDGCGI-LRRMTPIHNVKFEGKRYGGVTCVSDL------------PEKGGD--------- 364
                G  +R    +    ++ +     +  S L            P K  D         
Sbjct: 869  TSNGGFSVRSAYRVAMGLYQAENAVSASSNSQLQSFWKKLWHLPVPHKSFDEEKIILVVT 928

Query: 365  -------------GGSRSYYANAFLN-KNKSTLEMQVAPSCNPAV-------WCPPPKGF 424
                          G+      A +   N+  LE   A    PAV       W PPP   
Sbjct: 929  VAWAFWCNRNEIRHGAEKKSPEAIVQWVNRYLLEYSAATESVPAVREEVSVTWNPPPPSI 988

Query: 425  LKINTDAACTTSVGKSGIGILIRDDENRVQAAMIS--TQGLGSPLMAEILAIREGIRSAI 484
            LK+N D A T ++   G+G ++RD++ RV AAM       LG PL  E+ A   G++ A 
Sbjct: 989  LKVNVDGATTKNLNFVGVGAVVRDEQGRVVAAMSRKIPAPLG-PLEVEVKAFEAGLQLAK 1048

Query: 485  RMGFSKVVVESDSLEAVKLIENDEGCLNEVASWIVDIRRLCANFEDFRIQHVRRKGNMAA 502
             MG+  +++E DSL  V+ +       + + S IV ++  C++F    + HV+R+ N  A
Sbjct: 1049 DMGYQNIILEGDSLIIVRALCGISLSSSTIDSMIVGMQLFCSDFCTVYVSHVKRQENKPA 1108

BLAST of Moc05g09240 vs. ExPASy TrEMBL
Match: A0A6J1DZK3 (uncharacterized protein LOC111024968 OS=Momordica charantia OX=3673 GN=LOC111024968 PE=4 SV=1)

HSP 1 Score: 133.3 bits (334), Expect = 3.0e-27
Identity = 70/165 (42.42%), Postives = 99/165 (60.00%), Query Frame = 0

Query: 355 WCPPPKGFLKINTDAACTTSVGKSGIGILIRDDENRVQAAMISTQGLGSPLMAEILAIRE 414
           W PP  G +K+NTDAA   S   SG+G+L+R+    +  AM+    + +PL+A+ILAIRE
Sbjct: 67  WRPPLGGVVKMNTDAA--VSEEGSGVGVLLREGNAEIVGAMVDFHMVKTPLLAKILAIRE 126

Query: 415 GIRSAIRMGFSKVVVESDSLEAVKLIENDEGCLNEVASWIVDIRRLCANFEDFRIQHVRR 474
           G+  A R+G  +VVVE+DSLEA+ LI +      E  SW+ DIR    +F++   QHV R
Sbjct: 127 GLSLATRLGVHRVVVETDSLEALNLIGDKSPWKGEAVSWVEDIRAFARDFQEICFQHVFR 186

Query: 475 KGNMAAHILARNSISISRPFLWLYDFPVWLLSRVKADGLTPMAFM 520
           + N  A+ L R +IS+   FLW  DFP+WL    +A     +A M
Sbjct: 187 ESNAVAYHLGRETISLRCGFLWRLDFPIWLGRLAEAGKQNSVALM 229

BLAST of Moc05g09240 vs. ExPASy TrEMBL
Match: A0A803QEQ9 (Uncharacterized protein OS=Cannabis sativa OX=3483 PE=4 SV=1)

HSP 1 Score: 121.7 bits (304), Expect = 9.0e-24
Identity = 141/662 (21.30%), Postives = 241/662 (36.40%), Query Frame = 0

Query: 52  MKSFAVCGGSP---------SCATALGKWGRDFNRGFKRQIKKIWLKIAHIYN--EPPPW 111
           + +++ C  SP         +CA++L KW        K++IK+    ++ + N     P 
Sbjct: 167 LANWSSCNSSPLLQLTTNISNCASSLQKWHLSNFGNLKQEIKESHQHVSALQNSSSTDPQ 226

Query: 112 DFAAIHSLDRELDDLLESDEIYWKQRSREDWLKWGDRNSKWFHKKATIRKKRNSIRGI-- 171
            FAA+ + +  LD+LL  +E YW QR+R  W+K GD N+K+FH++A  R   N I+ +  
Sbjct: 227 HFAALKNSELILDELLAKEEDYWHQRARISWMKSGDSNTKFFHQRANARSINNRIKKLRD 286

Query: 172 EVEGVWTEEPQLID-----------NGGDERKAHCSIYQQRRTTTHPTFGK--------- 231
           E     T EP L+D           + G    A  +I +   T  +   G+         
Sbjct: 287 EAGNTQTSEPTLLDIIQTYFQSIFRSQGVHDHAINAILEVIPTPINEQSGETISAPYTEA 346

Query: 232 ---------------GWFGVVICCLWEV---------------VGDGSSINFFKDPWIPR 291
                          G  G      W                 VG+G+ I    DPW+P 
Sbjct: 347 DVFTALNSMAEDKSPGVDGACPSLTWRSIVWGKELLAKGLRWRVGNGNRIICKSDPWLPG 406

Query: 292 ETTFRPLSPCLDLSIIFWVADFVTSSRNWDVQKLRSIVGEEDVATISNIPIS-------- 351
            T F P +  +       VAD +T  R WD+  + +  G+ D+  I +IP++        
Sbjct: 407 HTEFTPFN-FIGRDNSLQVADLITQHRQWDLTAISANFGQADIDRILSIPLAIYPSDDML 466

Query: 352 ------------------------------------------------------------ 411
                                                                       
Sbjct: 467 IWNGTNSGNYMVKSGYYFASSLAELNDPGSTFSSENWWTKFWKLHLPSKLRIFVWKVYHN 526

Query: 412 --AVGVQIDG--------CGILR-RMTPIHNVKFEGKRYGGVTCVSDL-----------P 471
              V  +++         C + + +   I++  F   R   V  +S L           P
Sbjct: 527 VLPVAAELNRKHIAESPFCPLCKMQRESINHALFLCSRAKEVWSLSHLHLNFKLAATSTP 586

Query: 472 EK-----GGDGGSRSY-------------------------------YANAFLNKNKSTL 507
           E+       +  ++ +                               +A  +L K +S  
Sbjct: 587 EEFLLYASANSSTQEFELFLTICWSIWYERNAEYHGKLPKLAAAILVFATQYLIKYQSA- 646

BLAST of Moc05g09240 vs. ExPASy TrEMBL
Match: A0A7J6FM99 (CCHC-type domain-containing protein OS=Cannabis sativa OX=3483 GN=G4B88_030861 PE=4 SV=1)

HSP 1 Score: 119.4 bits (298), Expect = 4.5e-23
Identity = 75/214 (35.05%), Postives = 114/214 (53.27%), Query Frame = 0

Query: 64  CATALGKWGRDFNRGFKRQIKKIWLKIAHIYNEPPPW-DFAAIHSLDRELDDLLESDEIY 123
           C   LG+W +D      R++++   ++  + +   P      +  L+  L+DLL  +E Y
Sbjct: 666 CVERLGEWNKDKFGSIPRRVRETQKQLDDLLSVAAPLVRMDEVKRLETTLNDLLSREECY 725

Query: 124 WKQRSREDWLKWGDRNSKWFHKKATIRKKRNSIRGIEVEGVWTEEPQLIDNGGD-----E 183
           WK RSR DWL  GDRN+K+FH KAT RKK+N+I    VE + TE+ + +   GD     E
Sbjct: 726 WKLRSRADWLAMGDRNTKYFHNKATGRKKKNAI----VE-MMTEDGRKLTLEGDIVSEIE 785

Query: 184 R-------KAHCSIYQQRR--TTTHPTFGKGWFGVVICCLWEVVGDGSSINFFKDPWIPR 243
           R        A  S+ Q     T+    FG          + +VVGDG S++ F+DPW+PR
Sbjct: 786 RYFGTIFSSASPSVQQVENGSTSIEARFG----------MRKVVGDGQSVDAFRDPWLPR 845

Query: 244 ETTFRPLSPCLDLSIIFWVADFVTSSRNWDVQKL 263
             TFRP+SP  + S++  V++ +    +WDV+ L
Sbjct: 846 PRTFRPISPAPNASVM--VSELMGVDGSWDVRSL 862

BLAST of Moc05g09240 vs. ExPASy TrEMBL
Match: A0A7J6FHV6 (Uncharacterized protein OS=Cannabis sativa OX=3483 GN=F8388_007366 PE=4 SV=1)

HSP 1 Score: 119.4 bits (298), Expect = 4.5e-23
Identity = 75/214 (35.05%), Postives = 114/214 (53.27%), Query Frame = 0

Query: 64  CATALGKWGRDFNRGFKRQIKKIWLKIAHIYNEPPPW-DFAAIHSLDRELDDLLESDEIY 123
           C   LG+W +D      R++++   ++  + +   P      +  L+  L+DLL  +E Y
Sbjct: 388 CVERLGEWNKDKFGSIPRRVRETQKQLDDLLSVAAPLVRMDEVKRLETTLNDLLSREECY 447

Query: 124 WKQRSREDWLKWGDRNSKWFHKKATIRKKRNSIRGIEVEGVWTEEPQLIDNGGD-----E 183
           WK RSR DWL  GDRN+K+FH KAT RKK+N+I    VE + TE+ + +   GD     E
Sbjct: 448 WKLRSRADWLAMGDRNTKYFHNKATGRKKKNAI----VE-MMTEDGRKLTLEGDIVSEIE 507

Query: 184 R-------KAHCSIYQQRR--TTTHPTFGKGWFGVVICCLWEVVGDGSSINFFKDPWIPR 243
           R        A  S+ Q     T+    FG          + +VVGDG S++ F+DPW+PR
Sbjct: 508 RYFGTIFSSASPSVQQVENGSTSIEARFG----------MRKVVGDGQSVDAFRDPWLPR 567

Query: 244 ETTFRPLSPCLDLSIIFWVADFVTSSRNWDVQKL 263
             TFRP+SP  + S++  V++ +    +WDV+ L
Sbjct: 568 PRTFRPISPAPNASVM--VSELMGVDGSWDVRSL 584

BLAST of Moc05g09240 vs. ExPASy TrEMBL
Match: A0A803QDL0 (Uncharacterized protein OS=Cannabis sativa OX=3483 PE=4 SV=1)

HSP 1 Score: 117.5 bits (293), Expect = 1.7e-22
Identity = 137/566 (24.20%), Postives = 215/566 (37.99%), Query Frame = 0

Query: 64  CATALGKWGRDFNRGFKRQIKKIWLKIAHIYNEP--PPWDFAAIHSLDRELDDLLESDEI 123
           C +AL  W ++ N   K +I  +  +IA I + P     +   +  L  +LD LL  +EI
Sbjct: 150 CISALQSWNKNLNLSLKSRISDLQHEIARIQDLPFTDQDNQFRLQCLQSQLDALLYKEEI 209

Query: 124 YWKQRSREDWLKWGDRNSKWFHKKATIRKKRNSIRGIEVEGVWTEEPQLIDNGGDERKAH 183
           YWKQRSR  WLK GD+N+K+FH+ A+ RKK N+I+ ++      +  +++ +  D     
Sbjct: 210 YWKQRSRTHWLKAGDKNTKFFHRFASKRKKNNTIKFLK-----DDNQRIVSSHSDMSNLL 269

Query: 184 CSIYQQRRTTTHPTFGKGWFGVVICCL-----------------WEVVGDGSSINFFKDP 243
            S +      + P        +++ CL                  + VGDGS I   +D 
Sbjct: 270 VSYFND--LFSSPGSDADAVHLILDCLGPPLDDLDYAFLDAPFSTKEVGDGSDIRTIEDH 329

Query: 244 WIPRETTFRPLSPCLDLSIIFWVADFVTSSRNWDVQKLRSIVGEEDVATISNIPISAVGV 303
           WIP    F+  S  L  S   +++ F+T+S +WDV KL +      V  I ++P+     
Sbjct: 330 WIP-NNRFKFFSDNLPPSP--FLSYFITASGDWDVAKLTNCFSAPLVDEILSVPVLGEFG 389

Query: 304 QID------GCGILRRMTPIH----------------NVKFEGKRYG------------- 363
           + D        G     T  H                + KF  K +              
Sbjct: 390 KDDIIWGHHSSGEFTVKTAYHLAFSSQDLPSSSSFFASKKFWSKIWNSKIPPKVKVFIWR 449

Query: 364 ------------GVTCVSDLP----------------------EKGGDGGSRSYYAN--- 423
                          CV D P                       K       + + +   
Sbjct: 450 MLSNALPVSFSLNKRCVIDSPLCPLCKIHPETVKHALLDCSRSRKAWKSSRNNIFHHNLC 509

Query: 424 -----------AFLNKNKSTLEMQVAP----SCNPAVWCPPPKGFLKINTDAACTTSVGK 483
                       F  K   + + QV P    S    +   PP+G  +I TDAA   +  K
Sbjct: 510 NQPLDVYDWSVTFFFKYLDSQQDQVLPCASNSDQTILQQFPPEGDFQIFTDAAIDLNRQK 569

Query: 484 SGIGILIRDDENRVQAAMIST-QGLGSPLMAEILAIREGIRSAIRMGFSKVVVESDSLEA 515
             IG+ + +   +V A +     G  SP++AE  A+   ++ A  +     V+++D    
Sbjct: 570 HSIGVAVLNHSGQVVAGLAKPFSGCVSPMVAEAKAVVHALQWAYSICLPVDVLKTDCKSI 629

BLAST of Moc05g09240 vs. TAIR 10
Match: AT4G29090.1 (Ribonuclease H-like superfamily protein )

HSP 1 Score: 72.8 bits (177), Expect = 9.3e-13
Identity = 49/154 (31.82%), Postives = 79/154 (51.30%), Query Frame = 0

Query: 355 WCPPPKGFLKINTDAACTTSVGKSGIGILIRDDENRVQ-AAMISTQGLGSPLMAEILAIR 414
           W PPP  ++K NTDA       + GIG ++R+++  V+     +   L S L AE+ A+R
Sbjct: 420 WRPPPHQWVKCNTDATWNRDNERCGIGWVLRNEKGEVKWMGARALPKLKSVLEAELEAMR 479

Query: 415 EGIRSAIRMGFSKVVVESDSLEAVKLIENDEGCLNEVASWIVDIRRLCANFEDFRIQHVR 474
             + S  R  ++ V+ ESDS   ++++ NDE     +   I D++RL + F + +   + 
Sbjct: 480 WAVLSLSRFQYNYVIFESDSQVLIEILNNDE-IWPSLKPTIQDLQRLLSQFTEVKFVFIP 539

Query: 475 RKGNMAAHILARNSISISRPFLWLYDF-PVWLLS 507
           R+GN  A  +AR S+S       LY   P W  S
Sbjct: 540 REGNTLAERVARESLSFLNYDPKLYSIVPSWARS 572

BLAST of Moc05g09240 vs. TAIR 10
Match: AT5G42965.1 (Polynucleotidyl transferase, ribonuclease H-like superfamily protein )

HSP 1 Score: 57.4 bits (137), Expect = 4.0e-08
Identity = 32/86 (37.21%), Postives = 49/86 (56.98%), Query Frame = 0

Query: 405 LMAEILAIREGIRSAIRMGFSKVVVESDSLEAVKLIENDEGCLNEVASWIVDIRRLCANF 464
           L  E+ A+R    S  R  + KV+ ESDS   V L+ +D    N +A  I DIR +   F
Sbjct: 16  LEVELEAMRWAALSMSRFNYKKVIFESDSQHLVSLLGDDTDYPN-LAPRIQDIRHIFQQF 75

Query: 465 EDFRIQHVRRKGNMAAHILARNSISI 491
           E+ ++ H++R+GN  A  +AR S+S+
Sbjct: 76  EEVKVMHIKREGNSVADRIARESLSL 100

BLAST of Moc05g09240 vs. TAIR 10
Match: AT3G09510.1 (Ribonuclease H-like superfamily protein )

HSP 1 Score: 57.0 bits (136), Expect = 5.3e-08
Identity = 40/155 (25.81%), Postives = 68/155 (43.87%), Query Frame = 0

Query: 351 NPAVWCPPPKGFLKINTDAACTTSVGKSGIGILIRDDENR-VQAAMISTQGLGSPLMAEI 410
           N   W  PP  ++K N DA       ++  G +IR+     +    +      +PL AE 
Sbjct: 322 NKIEWRNPPATYVKCNFDAGFDVQKLEATGGWIIRNHYGTPISWGSMKLAHTSNPLEAET 381

Query: 411 LAIREGIRSAIRMGFSKVVVESDSLEAVKLIENDEGCLNEVASWIVDIRRLCANFEDFRI 470
            A+   ++     G+++V +E D    + LI N     + +A+ + DI      F   + 
Sbjct: 382 KALLAALQQTWIRGYTQVFMEGDCQTLINLI-NGISFHSSLANHLEDISFWANKFASIQF 441

Query: 471 QHVRRKGNMAAHILARNSISISRPFLWLYDFPVWL 505
             +RRKGN  AH+LA+   + S  +      P+WL
Sbjct: 442 GFIRRKGNKLAHVLAKYGCTYSTFYSGSGSLPIWL 475

BLAST of Moc05g09240 vs. TAIR 10
Match: AT1G10000.1 (Ribonuclease H-like superfamily protein )

HSP 1 Score: 44.7 bits (104), Expect = 2.7e-04
Identity = 30/89 (33.71%), Postives = 45/89 (50.56%), Query Frame = 0

Query: 403 SPLMAEILAIREGIRSAIRMGFSKVVVESDSLEAVKLIENDEGCLNEVASWIVDIRRLCA 462
           SPL AE  AI+  +  A+++  S ++V SDS   V  + N    LNE+   +V+IR +  
Sbjct: 203 SPLAAEAWAIKSAMLHALQLERSDLLVLSDSKSIVDAL-NSNVSLNEIFGLLVEIRSIRN 262

Query: 463 NFEDFRIQHVRRKGNMAAHILARNSISIS 492
            F     Q + R  N  A   A+ S+ IS
Sbjct: 263 RFRSISFQFIPRLVNSIADAAAKLSLCIS 290

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022158489.16.2e-2742.42uncharacterized protein LOC111024968 [Momordica charantia][more]
KAF4371757.19.3e-2335.05hypothetical protein G4B88_030861 [Cannabis sativa][more]
KAF4370225.19.3e-2335.05hypothetical protein F8388_007366 [Cannabis sativa][more]
KAF2323497.11.6e-2222.05hypothetical protein GH714_035735 [Hevea brasiliensis][more]
XP_023897447.11.6e-2225.98uncharacterized protein LOC112009345 [Quercus suber][more]
Match NameE-valueIdentityDescription
Match NameE-valueIdentityDescription
A0A6J1DZK33.0e-2742.42uncharacterized protein LOC111024968 OS=Momordica charantia OX=3673 GN=LOC111024... [more]
A0A803QEQ99.0e-2421.30Uncharacterized protein OS=Cannabis sativa OX=3483 PE=4 SV=1[more]
A0A7J6FM994.5e-2335.05CCHC-type domain-containing protein OS=Cannabis sativa OX=3483 GN=G4B88_030861 P... [more]
A0A7J6FHV64.5e-2335.05Uncharacterized protein OS=Cannabis sativa OX=3483 GN=F8388_007366 PE=4 SV=1[more]
A0A803QDL01.7e-2224.20Uncharacterized protein OS=Cannabis sativa OX=3483 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
AT4G29090.19.3e-1331.82Ribonuclease H-like superfamily protein [more]
AT5G42965.14.0e-0837.21Polynucleotidyl transferase, ribonuclease H-like superfamily protein [more]
AT3G09510.15.3e-0825.81Ribonuclease H-like superfamily protein [more]
AT1G10000.12.7e-0433.71Ribonuclease H-like superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (OHB3-1) v2
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002156Ribonuclease H domainPFAMPF13456RVT_3coord: 366..486
e-value: 2.0E-25
score: 89.0
IPR002156Ribonuclease H domainPROSITEPS50879RNASE_Hcoord: 359..489
score: 9.98306
IPR036397Ribonuclease H superfamilyGENE3D3.30.420.10coord: 362..489
e-value: 1.7E-21
score: 78.6
NoneNo IPR availablePANTHERPTHR47723OS05G0353850 PROTEINcoord: 342..500
IPR044730Ribonuclease H-like domain, plant typeCDDcd06222RNase_H_likecoord: 365..485
e-value: 3.66031E-35
score: 125.89
IPR012337Ribonuclease H-like superfamilySUPERFAMILY53098Ribonuclease H-likecoord: 362..496

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Moc05g09240.1Moc05g09240.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0003676 nucleic acid binding
molecular_function GO:0004523 RNA-DNA hybrid ribonuclease activity