Moc05g09220 (gene) Bitter gourd (OHB3-1) v2

Overview
NameMoc05g09220
Typegene
OrganismMomordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
DescriptionProtein MAK16 homolog
Locationchr5: 7157575 .. 7163868 (+)
RNA-Seq ExpressionMoc05g09220
SyntenyMoc05g09220
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGCAGCACGACGAAGTCATATGGCAGGTCATCCGCCACAATCACTGCAGCTTCATGGCCAAGTAAGTTTTTTTTTTTCTTTTCCTTTTTTGCAATTGATATTTCTAATGTGTTCGTGGTTTTTCTTTGGAAAAATTATGACTGGGTTTGTGCTTTGGTAGGATTACGACTGGGAAGTTCTGTAGAAATCCGTATAACGTAACTGGGATATGTAATCGGAGCTCATGCCCTTTGGCTAACAGTCGCTATGCTACTATTCGCGATCATGACGGTATTTCCTGCTTCTGATTAATTCTCATTGGGTTTATTTGTGTGTTGTCGATGCGTTGATTTTATTCACTACAATTTAGAGCGTGCTGCACTCTTTTTGCACTATTGATTGATAAAAGTGGATCTTTGTACCTCTATGGTGTTATTCGCATTATTGTCTGCAGAAGTGAATAAAGAATATCAGTTTATGACCTTTGGATGTTGGCTCTTAAACTGAATTTGAGAAAATTGAAGCCGGTTTGATGACCTTCTATTCTTCTTTTCCTTCTTCTTCTTTTATTTATTATTTTTTAGTTCTTAGTTTTTGAGGCTCCAGTGCATTTAGGAACGATATCCAAGTTTTGGTGCTCGTTTCCAATGTTCAAAATGTTAATTGTATTAGTAATTCTATCATTTCCAGCTTATAATATTATAAAAACGACAAATTCTTTGAATGTTGTACTTACTAAGAAATAAGAAGTGGTTAGAACAAGGAAGCAGAAAATAGGAAACGGGATATTGTTACTAGATGGCCCCAAGTTATCCACATTCTATTCAATAGTTGTTGAGCATTTTAAAGAGCTTTAATATCATAACCTAAGCATTGCTCAACTAGTCAAGGTATATATCGTTGACCTAAAGGTTGGTTGGAAAGGAATCATAGACTGTTTAGGGGGTGGAGAAGTCAGTAGATTACGTTTGGGAGATCATTAGATTTAATACTTCGGTTTCGGGCTCCGTTTCTAAGTCCTTTTGTAATTATCCGTTAGGCCTTATTCTTTTGGATTGGAGTTATTTTCTTTAGGGGGCTTCCTCCTTCAGGGCTGGTTTTTTTCATTTCTCTCAATAAAAAGTGTTGTTTGGTATAAAAAAATAAATCATTGACCTAAAGGTTGGGGATTTGAATCCCCACCTTTACATGTTCATTGAATTCAAAATGAGCTTCAACATCGTATGTGAAACTTTATGGACTACAAGTAATTGTTGAATATAACAGCTTCAACCAACGTACGTACAACATTGTGAATGGATATAAGAAATAATGTCAAGTACAGTACCCATACTCCCCCTAAACAACTCGCATTATAAATGAAGTATAAAATAATAAAAATAACAAAGTTTAATCCAGCCTTCAAAACTTAATCATCTACTTCAATTTTAGTAGTGGTTTACAATTGGATCTACTTGATGGAGTAGTGGATGCGACATGGATGAGAGTACTTATTCATTCAAATTTGATGCCATGAAGTCTCTGCATCCAGACCATTCTCCCCATGAAAATATCTTCTTTTCCTGAGATACAATTCTTTTTTAAATAATTCTTCGTTTCTCATATAATATATGCCAGTGCCAATTATAAATCTCGCTAATTTAGCTACCTATTGATGACTTATTTTATTGAGTTAAATTAGCAAAATATTATTCTATTGTTACTGCAGGAGTTTTCTATCTTTATATGAAGACAATTGAAAGAGCTCATAAGCCAAATGAGTTGTGGGAAAGAGTTAAGTTGCCCAGAAATTATGAAAAGGCACTGGAAATTATAGATAAACATCTGGTAAGTAATCATCTAAGTCTATGCTCTGCATCTTAGTTTAATCTTTTACATTCTCTTTTTCAAATTTTTTAACTTATCTTTTTATGCTGTTGACAGATGTATTGGCCTAAGGTACTTGTACACAAAACAAAGCAACGATTGACTAAAATGACCCAAATGCGGATACGTATGAGGAAGCTTGCTTTGAAAACAAGGTTCAATATTTTCTGCACCTTTCTTTGTTTTTGAACTGAGGCTGTTGTTCTATTTGAACTTTTCATTGAATGATGAAAAAAGTTGTTCTACCTATGCTAACACATGGACATGGGTACTTGATAACATTCATTATGTAAATCTTATTTGCTTTCCTGACTCTGATTGAAAAAATGGCAAAATGAAAAGATCACTATTTATAATTTTAATATAGTATAGAGGGGTGCATTCAGTCCATATGTCATGCCAAAAGGAGACCAAAGTGCATTTGGTTCTTTTCATAATGCGATCAGTCAACTTCTAAGGGTAAAGCAGGGGTCCTTAAAGCGACTTTTATTTTTTGTAGCAGGCCAAGAATGTGTGTGATTGTAACCATATTTACCCTCAATAGCTCTTCTCCATAATGCATCTTTTTCAATCAAGAACCTCCATATCCATTCCGCTATGAGGGCTTAGTTTCTCAGTTCGACAATGCCAATACCAGTATTTTGCTTCTTTTAAATAAGAAACATCAGGTACTAGAGATAAAAAAGGAGCAACAACCTAAGGGCAGGGGCCAAGATAGTCAAGACTCCACAAGAACACCTTCACCTTCTTTGGACAGTTAAAACTTCCAAATCTTGGTTACGAAAGGAGCTTTAAGCTTAGGAGTAACTTCAGTTAACTTTAAAACGGAAGATTTGGTGGTAAATCACTTCTCACCATTAAAAGTCCATTCCACCTTGTCTTCTCCATTTCCCCAGCGAAAAGAATTCAGGATCTCCACCAATTGAATCCAACTATGCCACTCTCTATCCATAATTCCTCTTCTGAATCCCAGATTCCAATCTCCATGTTCATTGTCCCAGCAATCAGCCACCAATATCCGTACTTTCATATTCCTTGGGCAATTGACTCTGGTTCCAATTCACTGAATGAGATCCTTCCTCTCTTTTATCACTTCCCAATGTTCCCATAAGAAATCTTTGAAAATTTCCTCTACTGCTTTTGCAGTTGTAGATAAAATTGTAAAAAGAGATAAATAATATAAAAGGAGGTTGGAGAGAGTTGCTTGTGTGATGGTGAGACGACCTACTTTGGCAATATTGGAATGCTTCCATTTATGGAGGTCATGGTCAACTTGCTTAATGATTGGAGCCTAGAAAGATGAAGAACGATTCTTACCATTTAATGGGAGCCCGAGGAAAGTATTTCCAGAATCTCTGATTTGCTATAATTTATGTTAAGACTTAAAGCATCTTAAAAAATATCTACAATGATGAAGGGGTTGTCTATCCTCCTTTTATCATTAATATAGAAAAAAAGGTCATCACAAATTGTAAATGACTAATGGAAATAGATGAATTTCACATATTAAATCCATTAATGAGGCACTATGCGACCATGATAAAAAGGATGGGAGGAAGCAGATTCCCTTCTCTAAGGCCTTTTTTTTTTTTAACTTCATCGAGGTGTGGGGGTGGTGGTGGGAATTCAAATCTCCAACCTTAAGGAAGGTTACTGGTATCATCTAGTTGAGCTATGCTTCTTGTCTTTGGCCTTAGATGCTAAATTTTACCTCTAGGTCTTCCATTAATGATTAAGGAAAAAATATGTGCCAGATACACAAGCACGGAGCTATTTCCTCCATGTATCACCAATGATATTTTTGGGAGACTTTCCTGGTCTTGGATTTTTCTAGCACATTTTTTTTCCCTGTTTAATAAATAGAAATTGTATTGATCAAAAGGTCATATACAAAAGGATGGGAATGAGAAATCCCCAAGCAGCCAGAGACTTACAAAAAGCTTCTTAGTTGGCCCAAAAATGCAAAAAAAAAAAAAAAAACATAATTACAAAACTTTTAAGATAGGTAGATAGGTGACACCACAAGGAGCATGGAAGTGAATGAGGTCCCAAACACTAGTGCTGAAGTTTGCCCCTTCATAACTTCTTTGGTTTCTCTCATCCCACAAATGCTATAAAGAAGCAAACATTGCATTTATCCACAGAATTTGAGTTAGACGTTTGAAATTGCTGTCTATTATCTTGTAGTTCTGAACTCAGCATTGTTGAACTAAGACCATCTTAAAATCCTCAAAATCTTTGGGAGGACATTGTAGTATCTTTTTATTTATTTCTGTTTTTGGAAAATAGTATTGGTAACTTTAAAGTTTTAAATTGCAAGAGCTCGAGTGTTCTCGCTATTATGGTTTAACAGAGGAGAGGAAGTAAGAGACAATCTGAAGGATGAAATATTGTTCTAAAAGGTCATGTGGAGTGACAATTCTTCTCCTGAAATTATCTGGATTCCTCTCCCCTTCTCTTTCCCTCTTACTCCCTTGCACGTGTACCCTTAATTGAAATTTCTGATTGTAGTATAGCTGCTCTGCTTCTAAACCGCCAATATCTCTGTTTGGTTAATTATTTCTTACATTTCTTCTTCATCCATTCCTTATGTTCTATATTGTCATTTGATTGTCTGTCTGTACATAATAAAGAATTGTAGTCTCTCTTGTTTCCTTAATCCTGTTTTATGCATCTAATCTGATGCCTTAATGAGAAGTAGTTTATTATAAATGTTTAGAAGATTTGATTTCCTTGATCTAACTATGTATTACTTTAATGGCCGAACAGGGAGAAAATCATGACAACTCCCAGAAAAGAGATTAAAAGAGAAGCCAGAAGGGAGCAAAAGGCTGAGAAAGCAGCATTGTTGGATAAGGTTGTACTTTCAAGATTTTTGTTGAACTTTGGTATATTCTTGATATTGAAACCAATGAAGTTTATTTTGTTTCTGCAAGAAAGATTTTTTCTTTACTGAGTACTTAATGTGGGGAAACTGTATTGAAGATCTACTTTTCTGCAGAGCATTGAAAAGGAACTATTAGAACGCCTTAAGAAAGGAGTATATGGTGATATATACAACTATCCTGTCGAAGCATATAATCAAGTTCTTGAAATGGACGAATTACAGGCCGCCAGTGAAGAGGAGGAGGAGGTAGTACTGCAAAATTTCTATTAGTACCCTCGCCCTTTCCATTCCTTTGAGGGAGAGTCTGGGACTGATTAAGGATTATATATTCCATTATTTGATAATTTGTATTTTTGCTTGGAATAAGCAGGAACCTGAAATAGAATATGTTGAAGGATATGAAGAATTAGAAGAAGAAGAAGATATGGAAGATTTTGGTGGTCTTGCAATCCATAATCCCGAAGCAGATGAAGATAGTGGTAAAGCAGCCTTTAGAATAACAAATTATTTCAAGTTTTCAACAAATCATTCCAGTTCTTTCTTATTAGAGATGCACCAGCTCTATGTTTTTTTTGGATAATGTATACATAGATCTTGTAAAACCAGAAGTGAGGTTCTTTTTTATCCCGGTAAATAGGTGTAGAAACAAGAGGGTTTTGCTTGGTGTTATTAGAACTGAGTGTAAATGTTCTGATGATTCAGATGGAATAGATGAAGATCTTGAAGACACAGATGTTCCGCACAAGAGAGGTAGAAAGGAGTCTACTCTTTCTTTGAGGAAGCTAGAGAAAGATGCTCATGCCAAACTGAAGAAGAAAGCGAGGGTTATAGTTGAGGTAATAGTTAATTTCCTACCATCACTTGAACAGTCAGTCGGAAAAATTGCATTAATATAATTTGAATCTTTGCTGTTTTGTTAGTCTTGTGGACTTGTTCATTTCATAAGATGGTTAGTGATGGTTTTTAGGATAACTGACATTATTCATGCACTCTTGCCCCACTCTCGTATGGTTCCTATCTGCTGGATAAAGGAAACTACTTTTTTCTTCCATTTTCTGTCTGTGGTCATTAATTCCTGATGCATGAAGATTACAGAAAAAGCTATCTGAAACAGTACAAATTTTCTCTATTTTCTTTTAGTGTCACCATTAGATAGAGATTCAAATCACAACTTTTAAGAGATTCCAATGCCTTAGATGTCTTGGAACTATACTTATGTCAGCAATTTACCTCATATTGGTTGGACATTTGTAGTTTTCCATGATGCATCTAGTGCATAATAATTCATGTTTGTATGCAATCCATTTACTTGAATGACCGATTACTCGCCATACAGTAAAATTTTATTCTTGGAAGATAGGTTTTTCTAGCAATATGGTATGAACTGGATCTTATCATTGTGCATTTGTTTTGTGGCACGTTTTTTGAAATAGAACTATGATACATGGTATGCGTTTGTGCTACTTCCATTTTTCTTGTGGTTATGAACAAGGTTCCTTGTGCCATATCTTGTTAGGTTGAGCATGAAGATGCTGATGAGCGCCAAACAACTGTCCACTAG

mRNA sequence

ATGCAGCACGACGAAGTCATATGGCAGGTCATCCGCCACAATCACTGCAGCTTCATGGCCAAGATTACGACTGGGAAGTTCTGTAGAAATCCGTATAACGTAACTGGGATATGTAATCGGAGCTCATGCCCTTTGGCTAACAGTCGCTATGCTACTATTCGCGATCATGACGGAGTTTTCTATCTTTATATGAAGACAATTGAAAGAGCTCATAAGCCAAATGAGTTGTGGGAAAGAGTTAAGTTGCCCAGAAATTATGAAAAGGCACTGGAAATTATAGATAAACATCTGATGTATTGGCCTAAGGTACTTGTACACAAAACAAAGCAACGATTGACTAAAATGACCCAAATGCGGATACGTATGAGGAAGCTTGCTTTGAAAACAAGGGAGAAAATCATGACAACTCCCAGAAAAGAGATTAAAAGAGAAGCCAGAAGGGAGCAAAAGGCTGAGAAAGCAGCATTGTTGGATAAGAGCATTGAAAAGGAACTATTAGAACGCCTTAAGAAAGGAGTATATGGTGATATATACAACTATCCTGTCGAAGCATATAATCAAGTTCTTGAAATGGACGAATTACAGGCCGCCAGTGAAGAGGAGGAGGAGGAACCTGAAATAGAATATGTTGAAGGATATGAAGAATTAGAAGAAGAAGAAGATATGGAAGATTTTGGTGGTCTTGCAATCCATAATCCCGAAGCAGATGAAGATAGTGATGGAATAGATGAAGATCTTGAAGACACAGATGTTCCGCACAAGAGAGGTAGAAAGGAGTCTACTCTTTCTTTGAGGAAGCTAGAGAAAGATGCTCATGCCAAACTGAAGAAGAAAGCGAGGGTTATAGTTGAGGTTGAGCATGAAGATGCTGATGAGCGCCAAACAACTGTCCACTAG

Coding sequence (CDS)

ATGCAGCACGACGAAGTCATATGGCAGGTCATCCGCCACAATCACTGCAGCTTCATGGCCAAGATTACGACTGGGAAGTTCTGTAGAAATCCGTATAACGTAACTGGGATATGTAATCGGAGCTCATGCCCTTTGGCTAACAGTCGCTATGCTACTATTCGCGATCATGACGGAGTTTTCTATCTTTATATGAAGACAATTGAAAGAGCTCATAAGCCAAATGAGTTGTGGGAAAGAGTTAAGTTGCCCAGAAATTATGAAAAGGCACTGGAAATTATAGATAAACATCTGATGTATTGGCCTAAGGTACTTGTACACAAAACAAAGCAACGATTGACTAAAATGACCCAAATGCGGATACGTATGAGGAAGCTTGCTTTGAAAACAAGGGAGAAAATCATGACAACTCCCAGAAAAGAGATTAAAAGAGAAGCCAGAAGGGAGCAAAAGGCTGAGAAAGCAGCATTGTTGGATAAGAGCATTGAAAAGGAACTATTAGAACGCCTTAAGAAAGGAGTATATGGTGATATATACAACTATCCTGTCGAAGCATATAATCAAGTTCTTGAAATGGACGAATTACAGGCCGCCAGTGAAGAGGAGGAGGAGGAACCTGAAATAGAATATGTTGAAGGATATGAAGAATTAGAAGAAGAAGAAGATATGGAAGATTTTGGTGGTCTTGCAATCCATAATCCCGAAGCAGATGAAGATAGTGATGGAATAGATGAAGATCTTGAAGACACAGATGTTCCGCACAAGAGAGGTAGAAAGGAGTCTACTCTTTCTTTGAGGAAGCTAGAGAAAGATGCTCATGCCAAACTGAAGAAGAAAGCGAGGGTTATAGTTGAGGTTGAGCATGAAGATGCTGATGAGCGCCAAACAACTGTCCACTAG

Protein sequence

MQHDEVIWQVIRHNHCSFMAKITTGKFCRNPYNVTGICNRSSCPLANSRYATIRDHDGVFYLYMKTIERAHKPNELWERVKLPRNYEKALEIIDKHLMYWPKVLVHKTKQRLTKMTQMRIRMRKLALKTREKIMTTPRKEIKREARREQKAEKAALLDKSIEKELLERLKKGVYGDIYNYPVEAYNQVLEMDELQAASEEEEEEPEIEYVEGYEELEEEEDMEDFGGLAIHNPEADEDSDGIDEDLEDTDVPHKRGRKESTLSLRKLEKDAHAKLKKKARVIVEVEHEDADERQTTVH
Homology
BLAST of Moc05g09220 vs. NCBI nr
Match: XP_022145243.1 (protein MAK16 homolog A isoform X1 [Momordica charantia])

HSP 1 Score: 572.8 bits (1475), Expect = 1.7e-159
Identity = 298/298 (100.00%), Postives = 298/298 (100.00%), Query Frame = 0

Query: 1   MQHDEVIWQVIRHNHCSFMAKITTGKFCRNPYNVTGICNRSSCPLANSRYATIRDHDGVF 60
           MQHDEVIWQVIRHNHCSFMAKITTGKFCRNPYNVTGICNRSSCPLANSRYATIRDHDGVF
Sbjct: 1   MQHDEVIWQVIRHNHCSFMAKITTGKFCRNPYNVTGICNRSSCPLANSRYATIRDHDGVF 60

Query: 61  YLYMKTIERAHKPNELWERVKLPRNYEKALEIIDKHLMYWPKVLVHKTKQRLTKMTQMRI 120
           YLYMKTIERAHKPNELWERVKLPRNYEKALEIIDKHLMYWPKVLVHKTKQRLTKMTQMRI
Sbjct: 61  YLYMKTIERAHKPNELWERVKLPRNYEKALEIIDKHLMYWPKVLVHKTKQRLTKMTQMRI 120

Query: 121 RMRKLALKTREKIMTTPRKEIKREARREQKAEKAALLDKSIEKELLERLKKGVYGDIYNY 180
           RMRKLALKTREKIMTTPRKEIKREARREQKAEKAALLDKSIEKELLERLKKGVYGDIYNY
Sbjct: 121 RMRKLALKTREKIMTTPRKEIKREARREQKAEKAALLDKSIEKELLERLKKGVYGDIYNY 180

Query: 181 PVEAYNQVLEMDELQAASEEEEEEPEIEYVEGYEELEEEEDMEDFGGLAIHNPEADEDSD 240
           PVEAYNQVLEMDELQAASEEEEEEPEIEYVEGYEELEEEEDMEDFGGLAIHNPEADEDSD
Sbjct: 181 PVEAYNQVLEMDELQAASEEEEEEPEIEYVEGYEELEEEEDMEDFGGLAIHNPEADEDSD 240

Query: 241 GIDEDLEDTDVPHKRGRKESTLSLRKLEKDAHAKLKKKARVIVEVEHEDADERQTTVH 299
           GIDEDLEDTDVPHKRGRKESTLSLRKLEKDAHAKLKKKARVIVEVEHEDADERQTTVH
Sbjct: 241 GIDEDLEDTDVPHKRGRKESTLSLRKLEKDAHAKLKKKARVIVEVEHEDADERQTTVH 298

BLAST of Moc05g09220 vs. NCBI nr
Match: XP_022145244.1 (protein MAK16 homolog isoform X2 [Momordica charantia])

HSP 1 Score: 567.0 bits (1460), Expect = 9.6e-158
Identity = 297/298 (99.66%), Postives = 297/298 (99.66%), Query Frame = 0

Query: 1   MQHDEVIWQVIRHNHCSFMAKITTGKFCRNPYNVTGICNRSSCPLANSRYATIRDHDGVF 60
           MQHDEVIWQVIRHNHCSFMAKITTGKFCRNPYNVTGICNRSSCPLANSRYATIRDHDGVF
Sbjct: 1   MQHDEVIWQVIRHNHCSFMAKITTGKFCRNPYNVTGICNRSSCPLANSRYATIRDHDGVF 60

Query: 61  YLYMKTIERAHKPNELWERVKLPRNYEKALEIIDKHLMYWPKVLVHKTKQRLTKMTQMRI 120
           YLYMKTIERAHKPNELWERVKLPRNYEKALEIIDKHLMYWPKVLVHKTKQRLTKMTQMRI
Sbjct: 61  YLYMKTIERAHKPNELWERVKLPRNYEKALEIIDKHLMYWPKVLVHKTKQRLTKMTQMRI 120

Query: 121 RMRKLALKTREKIMTTPRKEIKREARREQKAEKAALLDKSIEKELLERLKKGVYGDIYNY 180
           RMRKLALKTREKIMTTPRKEIKREARREQKAEKAALLDKSIEKELLERLKKGVYGDIYNY
Sbjct: 121 RMRKLALKTREKIMTTPRKEIKREARREQKAEKAALLDKSIEKELLERLKKGVYGDIYNY 180

Query: 181 PVEAYNQVLEMDELQAASEEEEEEPEIEYVEGYEELEEEEDMEDFGGLAIHNPEADEDSD 240
           PVEAYNQVLEMDELQAAS EEEEEPEIEYVEGYEELEEEEDMEDFGGLAIHNPEADEDSD
Sbjct: 181 PVEAYNQVLEMDELQAAS-EEEEEPEIEYVEGYEELEEEEDMEDFGGLAIHNPEADEDSD 240

Query: 241 GIDEDLEDTDVPHKRGRKESTLSLRKLEKDAHAKLKKKARVIVEVEHEDADERQTTVH 299
           GIDEDLEDTDVPHKRGRKESTLSLRKLEKDAHAKLKKKARVIVEVEHEDADERQTTVH
Sbjct: 241 GIDEDLEDTDVPHKRGRKESTLSLRKLEKDAHAKLKKKARVIVEVEHEDADERQTTVH 297

BLAST of Moc05g09220 vs. NCBI nr
Match: XP_038905733.1 (protein MAK16 homolog isoform X2 [Benincasa hispida])

HSP 1 Score: 550.1 bits (1416), Expect = 1.2e-152
Identity = 286/298 (95.97%), Postives = 294/298 (98.66%), Query Frame = 0

Query: 1   MQHDEVIWQVIRHNHCSFMAKITTGKFCRNPYNVTGICNRSSCPLANSRYATIRDHDGVF 60
           MQHDEVIWQVIRHNHCSFMAKITTGKFCRNPYNVTGICNRSSCPLANSRYATIRDHDGVF
Sbjct: 1   MQHDEVIWQVIRHNHCSFMAKITTGKFCRNPYNVTGICNRSSCPLANSRYATIRDHDGVF 60

Query: 61  YLYMKTIERAHKPNELWERVKLPRNYEKALEIIDKHLMYWPKVLVHKTKQRLTKMTQMRI 120
           YLYMKTIERAHKPNELWERVKLPRNYEKALEIIDKHLMYWPK+LVHKTKQRLTKMTQMRI
Sbjct: 61  YLYMKTIERAHKPNELWERVKLPRNYEKALEIIDKHLMYWPKILVHKTKQRLTKMTQMRI 120

Query: 121 RMRKLALKTREKIMTTPRKEIKREARREQKAEKAALLDKSIEKELLERLKKGVYGDIYNY 180
           RMRKLALKTREKIMTTPRKEIKREARREQKAEKAALLDKSIEKELLERLKKGVYGDIYNY
Sbjct: 121 RMRKLALKTREKIMTTPRKEIKREARREQKAEKAALLDKSIEKELLERLKKGVYGDIYNY 180

Query: 181 PVEAYNQVLEMDELQAASEEEEEEPEIEYVEGYEELEEEEDMEDFGGLAIHNPEADEDSD 240
           PVEAYN+VLEM+ELQAAS EEEEEPEIEYVEGYEELEEEEDMEDFGGLAIHNPEADEDSD
Sbjct: 181 PVEAYNEVLEMEELQAAS-EEEEEPEIEYVEGYEELEEEEDMEDFGGLAIHNPEADEDSD 240

Query: 241 GIDEDLEDTDVPHKRGRKESTLSLRKLEKDAHAKLKKKARVIVEVEHEDADERQTTVH 299
           G+DEDLED D+PHKRG+KEST SLRKLEKDA AKLKKKARVIVEVE+E+ADERQTTVH
Sbjct: 241 GLDEDLEDIDIPHKRGKKESTTSLRKLEKDARAKLKKKARVIVEVENEEADERQTTVH 297

BLAST of Moc05g09220 vs. NCBI nr
Match: XP_023539083.1 (protein MAK16 homolog [Cucurbita pepo subsp. pepo])

HSP 1 Score: 548.1 bits (1411), Expect = 4.6e-152
Identity = 286/298 (95.97%), Postives = 292/298 (97.99%), Query Frame = 0

Query: 1   MQHDEVIWQVIRHNHCSFMAKITTGKFCRNPYNVTGICNRSSCPLANSRYATIRDHDGVF 60
           MQHDEVIWQVIRHNHCSFMAKI+TG FCRNPYNVTGICNRSSCPLANSRYATIRDHDGVF
Sbjct: 1   MQHDEVIWQVIRHNHCSFMAKISTGNFCRNPYNVTGICNRSSCPLANSRYATIRDHDGVF 60

Query: 61  YLYMKTIERAHKPNELWERVKLPRNYEKALEIIDKHLMYWPKVLVHKTKQRLTKMTQMRI 120
           YLYMKTIERAHKPNELWERVKLPRNYEKALEIIDKHLMYWPK+LVHKTKQRLTKMTQMRI
Sbjct: 61  YLYMKTIERAHKPNELWERVKLPRNYEKALEIIDKHLMYWPKILVHKTKQRLTKMTQMRI 120

Query: 121 RMRKLALKTREKIMTTPRKEIKREARREQKAEKAALLDKSIEKELLERLKKGVYGDIYNY 180
           RMRKLALKTREKIMTTPRKEIKREARREQKAEKAALLDKSIEKELLERLKKGVYGDIYNY
Sbjct: 121 RMRKLALKTREKIMTTPRKEIKREARREQKAEKAALLDKSIEKELLERLKKGVYGDIYNY 180

Query: 181 PVEAYNQVLEMDELQAASEEEEEEPEIEYVEGYEELEEEEDMEDFGGLAIHNPEADEDSD 240
           PVEAYN+VLEM+ELQAAS EEEEEPEIEYVEGYEELEEEEDMEDFGGLAIHNPEADEDSD
Sbjct: 181 PVEAYNEVLEMEELQAAS-EEEEEPEIEYVEGYEELEEEEDMEDFGGLAIHNPEADEDSD 240

Query: 241 GIDEDLEDTDVPHKRGRKESTLSLRKLEKDAHAKLKKKARVIVEVEHEDADERQTTVH 299
           GIDEDLED DVP KRGRKEST SLRK+EKDA AKLKKKA+VIVEVEHEDADERQTTVH
Sbjct: 241 GIDEDLEDVDVPRKRGRKESTYSLRKVEKDARAKLKKKAKVIVEVEHEDADERQTTVH 297

BLAST of Moc05g09220 vs. NCBI nr
Match: XP_022956306.1 (protein MAK16 homolog [Cucurbita moschata] >XP_022956307.1 protein MAK16 homolog [Cucurbita moschata] >KAG7018456.1 Protein MAK16-like A [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 547.7 bits (1410), Expect = 6.0e-152
Identity = 288/298 (96.64%), Postives = 293/298 (98.32%), Query Frame = 0

Query: 1   MQHDEVIWQVIRHNHCSFMAKITTGKFCRNPYNVTGICNRSSCPLANSRYATIRDHDGVF 60
           MQHDEVIWQVIRHNHCSFMAKI+TG FCRNPYNVTGICNRSSCPLANSRYATIRDHDGVF
Sbjct: 1   MQHDEVIWQVIRHNHCSFMAKISTGNFCRNPYNVTGICNRSSCPLANSRYATIRDHDGVF 60

Query: 61  YLYMKTIERAHKPNELWERVKLPRNYEKALEIIDKHLMYWPKVLVHKTKQRLTKMTQMRI 120
           YLYMKTIERAHKPNELWERVKLPRNYEKALEIIDKHLMYWPK+LVHKTKQRLTKMTQMRI
Sbjct: 61  YLYMKTIERAHKPNELWERVKLPRNYEKALEIIDKHLMYWPKILVHKTKQRLTKMTQMRI 120

Query: 121 RMRKLALKTREKIMTTPRKEIKREARREQKAEKAALLDKSIEKELLERLKKGVYGDIYNY 180
           RMRKLALKTREKIMTTPRKEIKREARREQKAEKAALLDKSIEKELLERLKKGVYGDIYNY
Sbjct: 121 RMRKLALKTREKIMTTPRKEIKREARREQKAEKAALLDKSIEKELLERLKKGVYGDIYNY 180

Query: 181 PVEAYNQVLEMDELQAASEEEEEEPEIEYVEGYEELEEEEDMEDFGGLAIHNPEADEDSD 240
           PVEAYN+VLEM+ELQAAS EEEEEPEIEYVEGYEELEEEEDMEDFGGLAIHNPEADEDSD
Sbjct: 181 PVEAYNEVLEMEELQAAS-EEEEEPEIEYVEGYEELEEEEDMEDFGGLAIHNPEADEDSD 240

Query: 241 GIDEDLEDTDVPHKRGRKESTLSLRKLEKDAHAKLKKKARVIVEVEHEDADERQTTVH 299
           GIDEDLED DVPHKRGRK+ST SLRKLEKDA AKLKKKARVIVEVEHEDADERQTTVH
Sbjct: 241 GIDEDLEDIDVPHKRGRKDST-SLRKLEKDARAKLKKKARVIVEVEHEDADERQTTVH 296

BLAST of Moc05g09220 vs. ExPASy Swiss-Prot
Match: Q66L33 (Protein MAK16 homolog A OS=Xenopus laevis OX=8355 GN=mak16-a PE=2 SV=1)

HSP 1 Score: 240.4 bits (612), Expect = 2.7e-62
Identity = 146/306 (47.71%), Postives = 202/306 (66.01%), Query Frame = 0

Query: 1   MQHDEVIWQVIRHNH-CSFMAKITTGKFCRNPYNVTGICNRSSCPLANSRYATIRDHDGV 60
           MQHD+VIW V+ +   CSF  K  T  FCRN YN+TG+CNRS+CPLANS+YATI++  G+
Sbjct: 1   MQHDDVIWDVVGNKQFCSFKIKTKTQNFCRNEYNITGLCNRSACPLANSQYATIKEEKGI 60

Query: 61  FYLYMKTIERAHKPNELWERVKLPRNYEKALEIIDKHLMYWPKVLVHKTKQRLTKMTQMR 120
            YLYMKTIERA  P  +WERV+L +NYE+ALE ID++L+YWP+ + HK KQR TK+TQ  
Sbjct: 61  CYLYMKTIERAAFPARMWERVRLSKNYEQALEQIDENLIYWPRFIRHKCKQRFTKITQYL 120

Query: 121 IRMRKLALKTREKIMTTPRKEIKREARREQKAEKAALLDKSIEKELLERLKKGVYGDIYN 180
           IR+RKL LK + K++   RK  +RE RRE+KA  AA LD +IEKELLERLK+G YGDIYN
Sbjct: 121 IRIRKLTLKRQRKLVPLSRKVERREKRREEKALVAAQLDNAIEKELLERLKQGAYGDIYN 180

Query: 181 YPVEAYNQVLEMDELQAAS-----EEEEEEPEI---EYVEGYEELEEEEDMEDFGGLAIH 240
           +P++A+++ LE  E  +AS     EEEE++ EI   E+VE  ++  +E D+ DF  +   
Sbjct: 181 FPIQAFDKALEQQEDASASESSADEEEEDDEEIGKREFVE--DDNVDESDLSDFEDMDKL 240

Query: 241 NPEADEDSDGIDEDLEDTDVPHKRGRKESTLSLRKLEKDAHAKLKKKARVIVEVEHEDAD 298
               DED    + + E ++   + G+ +S     K     H  ++K+ RV +E E E   
Sbjct: 241 GASGDEDKHSSESEEESSEEEEEAGKAKSK---GKAPLKGHL-IRKRPRVEIEYEQETEP 300

BLAST of Moc05g09220 vs. ExPASy Swiss-Prot
Match: Q6NYD4 (Protein MAK16 homolog OS=Danio rerio OX=7955 GN=mak16 PE=2 SV=1)

HSP 1 Score: 236.9 bits (603), Expect = 3.0e-61
Identity = 143/298 (47.99%), Postives = 198/298 (66.44%), Query Frame = 0

Query: 1   MQHDEVIWQVI-RHNHCSFMAKITTGKFCRNPYNVTGICNRSSCPLANSRYATIRDHDGV 60
           MQHD+VIW +I   + CS+  K  T +FCRN YN+TG+CNRSSCPLANS+YATIR+  G 
Sbjct: 1   MQHDDVIWDLIGNKSFCSYKVKTKTQQFCRNEYNITGLCNRSSCPLANSQYATIREEKGQ 60

Query: 61  FYLYMKTIERAHKPNELWERVKLPRNYEKALEIIDKHLMYWPKVLVHKTKQRLTKMTQMR 120
            +LYMK IERA  P+ +WE+VKL RNY KALE ID++L+YWP+ + HK KQRLTK+TQ  
Sbjct: 61  CFLYMKVIERAAFPSRMWEKVKLDRNYAKALEQIDENLIYWPRFIRHKCKQRLTKITQYL 120

Query: 121 IRMRKLALKTREKIMTTPRKEIKREARREQKAEKAALLDKSIEKELLERLKKGVYGDIYN 180
           IR+RKL LK + K++   RK  +RE RRE+KA  AA L+ +IEKELL+RLK+G YGDIYN
Sbjct: 121 IRIRKLTLKRQRKLVPLSRKVERREKRREEKALIAAQLENAIEKELLDRLKQGTYGDIYN 180

Query: 181 YPVEAYNQVLEMDELQAASEEEEEEPEIEYVEGYEELEEEE----DMEDFGGLAIHNPEA 240
           +P+ A+++ +E  + ++ SEEEEEE + E     E + E+E    D+ DF  L   N   
Sbjct: 181 FPINAFDKAMEKQDEESESEEEEEEEDEEESGTREFIAEDEFEESDLSDFEEL---NNLK 240

Query: 241 DEDSDGIDEDLEDTDVPHKRGRKESTLSLRKLEKDAHAK-LKKKARVIVEVEHEDADE 293
           D   + ++++ E ++   +   +E   S  K +  A  K   +K R  VE+E+E   E
Sbjct: 241 DSSDEEVNDEEESSEESEEESEEEEVKSKAKSKGKAPLKGPSRKKRAYVEIEYEQETE 295

BLAST of Moc05g09220 vs. ExPASy Swiss-Prot
Match: Q7ZYG5 (Protein MAK16 homolog B OS=Xenopus laevis OX=8355 GN=mak16-b PE=2 SV=1)

HSP 1 Score: 236.1 bits (601), Expect = 5.1e-61
Identity = 148/305 (48.52%), Postives = 197/305 (64.59%), Query Frame = 0

Query: 1   MQHDEVIWQVIRHNH-CSFMAKITTGKFCRNPYNVTGICNRSSCPLANSRYATIRDHDGV 60
           MQHD+VIW V+ +   CSF  K  T  FCRN +N+TG+CNRS+CPLANS+YATI++  G+
Sbjct: 1   MQHDDVIWDVVGNKQFCSFKIKTKTQNFCRNEFNITGLCNRSACPLANSQYATIKEEKGI 60

Query: 61  FYLYMKTIERAHKPNELWERVKLPRNYEKALEIIDKHLMYWPKVLVHKTKQRLTKMTQMR 120
            YLYMKTIERA  P  +WERV+L +NYE+ALE ID++L+YWP+ + HK KQR TK+TQ  
Sbjct: 61  CYLYMKTIERAAFPARMWERVRLSKNYEQALEQIDENLIYWPRFIRHKCKQRFTKITQYL 120

Query: 121 IRMRKLALKTREKIMTTPRKEIKREARREQKAEKAALLDKSIEKELLERLKKGVYGDIYN 180
           IR+RKL LK + K++   RK  +RE RRE+KA  AA LD +IEKELLERLK+G YGDIYN
Sbjct: 121 IRIRKLTLKRQRKLVPLSRKVERREKRREEKALVAAQLDNAIEKELLERLKQGAYGDIYN 180

Query: 181 YPVEAYNQVLEMDELQAASEEEEEEPEIEYVEGYEELEEEEDMEDFGGLAIHNPEADEDS 240
           +P++A+++ LE  +  +ASE  +EE E +   G  E  E++D+E+       +    ED 
Sbjct: 181 FPIQAFDKALEQQDEASASESSDEEEEDDEESGKREFVEDDDVEE------SDLSDFEDM 240

Query: 241 DGIDEDLEDTDVPHKRGRKEST-LSLRKLEKDAHAKLKKKA---------RVIVEVEHED 295
           D +     D D P     +ES+     K EK   AK K KA         R  VE+E+E 
Sbjct: 241 DKLGAS-SDEDKPSSESEEESSGEEDEKEEKAPKAKSKGKAPLKGHLIRKRPHVEIEYEQ 298

BLAST of Moc05g09220 vs. ExPASy Swiss-Prot
Match: Q6P7N1 (Protein MAK16 homolog OS=Xenopus tropicalis OX=8364 GN=mak16 PE=2 SV=1)

HSP 1 Score: 235.3 bits (599), Expect = 8.7e-61
Identity = 149/305 (48.85%), Postives = 194/305 (63.61%), Query Frame = 0

Query: 1   MQHDEVIWQVIRHNH-CSFMAKITTGKFCRNPYNVTGICNRSSCPLANSRYATIRDHDGV 60
           MQHD+VIW V+ +   CSF  K  T  FCRN YN+TG+CNRS+CPLANS+YATI++  G+
Sbjct: 1   MQHDDVIWDVVGNKQFCSFKIKTKTQNFCRNEYNITGLCNRSACPLANSQYATIKEEKGI 60

Query: 61  FYLYMKTIERAHKPNELWERVKLPRNYEKALEIIDKHLMYWPKVLVHKTKQRLTKMTQMR 120
            YLYMKTIERA  P  +WERV+L +NYE+ALE ID+ L+YWP+ + HK KQR TK+TQ  
Sbjct: 61  CYLYMKTIERAAFPARMWERVRLSKNYEQALEQIDESLIYWPRFIRHKCKQRFTKITQYL 120

Query: 121 IRMRKLALKTREKIMTTPRKEIKREARREQKAEKAALLDKSIEKELLERLKKGVYGDIYN 180
           IR+RKL LK + K++   RK  +RE RRE+KA  AA LD +IEKELLERLK+G YGDIYN
Sbjct: 121 IRIRKLTLKRQRKLVPLSRKVERREKRREEKALVAAQLDNAIEKELLERLKQGAYGDIYN 180

Query: 181 YPVEAYNQVLEMDELQAASE-EEEEEPEIEYVEGYEELEEEEDMEDFGGLAIHNPEADED 240
           +P++A+++ LE  E  +ASE  EEEE E +   G  E  E+ED+++            + 
Sbjct: 181 FPIQAFDKALEQQEDASASESSEEEEEEDDEESGKREFVEDEDVDE-----------SDL 240

Query: 241 SDGIDEDLEDTDVPHKRGRKESTLSLRKLEKDAHAKLKKKA---------RVIVEVEHED 295
           SD  D D        ++   E   S  + EK   AK K KA         R  VE+E+E 
Sbjct: 241 SDFEDMDKLGASSEEEKSSGEEESSEEEEEKAPKAKSKGKAPVKGQLIRKRQHVEIEYEQ 294

BLAST of Moc05g09220 vs. ExPASy Swiss-Prot
Match: Q8BGS0 (Protein MAK16 homolog OS=Mus musculus OX=10090 GN=Mak16 PE=1 SV=1)

HSP 1 Score: 229.9 bits (585), Expect = 3.6e-59
Identity = 145/307 (47.23%), Postives = 196/307 (63.84%), Query Frame = 0

Query: 1   MQHDEVIWQVIRHNH-CSFMAKITTGKFCRNPYNVTGICNRSSCPLANSRYATIRDHDGV 60
           MQ D+VIW  + +   CSF  +  T  FCRN Y++TG+CNRSSCPLANS+YATI++  G 
Sbjct: 1   MQSDDVIWNTLGNKQFCSFKIRTKTQGFCRNEYSLTGLCNRSSCPLANSQYATIKEEKGQ 60

Query: 61  FYLYMKTIERAHKPNELWERVKLPRNYEKALEIIDKHLMYWPKVLVHKTKQRLTKMTQMR 120
            YLYMK IERA  P  LWERV+L +NYEKALE ID++L+YWP+ + HK KQR TK+TQ  
Sbjct: 61  CYLYMKVIERAAFPRRLWERVRLSKNYEKALEQIDENLIYWPRFIRHKCKQRFTKITQYL 120

Query: 121 IRMRKLALKTREKIMTTPRKEIKREARREQKAEKAALLDKSIEKELLERLKKGVYGDIYN 180
           IR+RKL LK + K++   +K  +RE RRE+KA  AA LD +IEKELLERLK+  YGDIYN
Sbjct: 121 IRIRKLTLKRQRKLVPLSKKVERREKRREEKALIAAQLDNAIEKELLERLKQDTYGDIYN 180

Query: 181 YPVEAYNQVLEMDELQAASEEEEEEPE---------IEYVEGYEELEEEEDMEDFGGLAI 240
           +P+ A+++ LE  E ++ SE+EEEE +          E+VE  +E  EE D+ DF  +  
Sbjct: 181 FPIHAFDKALEKQEAESDSEDEEEEEDEDEEEDVGKREFVE--DEEVEESDLSDFEDMDK 240

Query: 241 HNPEADEDSDGIDEDLEDTDVPHKRGRKESTLSLRKLEKDAHAKLKKKARVIVEVEHEDA 298
            N +++ED D  DE   D +    + + ++ L            L+KK R  VE+E+E  
Sbjct: 241 LNTDSEEDQD--DESSNDEEAHKAKHKGKAPLK---------GPLRKK-RAYVEIEYEQE 293

BLAST of Moc05g09220 vs. ExPASy TrEMBL
Match: A0A6J1CVF0 (Protein MAK16 homolog OS=Momordica charantia OX=3673 GN=LOC111014743 PE=3 SV=1)

HSP 1 Score: 572.8 bits (1475), Expect = 8.5e-160
Identity = 298/298 (100.00%), Postives = 298/298 (100.00%), Query Frame = 0

Query: 1   MQHDEVIWQVIRHNHCSFMAKITTGKFCRNPYNVTGICNRSSCPLANSRYATIRDHDGVF 60
           MQHDEVIWQVIRHNHCSFMAKITTGKFCRNPYNVTGICNRSSCPLANSRYATIRDHDGVF
Sbjct: 1   MQHDEVIWQVIRHNHCSFMAKITTGKFCRNPYNVTGICNRSSCPLANSRYATIRDHDGVF 60

Query: 61  YLYMKTIERAHKPNELWERVKLPRNYEKALEIIDKHLMYWPKVLVHKTKQRLTKMTQMRI 120
           YLYMKTIERAHKPNELWERVKLPRNYEKALEIIDKHLMYWPKVLVHKTKQRLTKMTQMRI
Sbjct: 61  YLYMKTIERAHKPNELWERVKLPRNYEKALEIIDKHLMYWPKVLVHKTKQRLTKMTQMRI 120

Query: 121 RMRKLALKTREKIMTTPRKEIKREARREQKAEKAALLDKSIEKELLERLKKGVYGDIYNY 180
           RMRKLALKTREKIMTTPRKEIKREARREQKAEKAALLDKSIEKELLERLKKGVYGDIYNY
Sbjct: 121 RMRKLALKTREKIMTTPRKEIKREARREQKAEKAALLDKSIEKELLERLKKGVYGDIYNY 180

Query: 181 PVEAYNQVLEMDELQAASEEEEEEPEIEYVEGYEELEEEEDMEDFGGLAIHNPEADEDSD 240
           PVEAYNQVLEMDELQAASEEEEEEPEIEYVEGYEELEEEEDMEDFGGLAIHNPEADEDSD
Sbjct: 181 PVEAYNQVLEMDELQAASEEEEEEPEIEYVEGYEELEEEEDMEDFGGLAIHNPEADEDSD 240

Query: 241 GIDEDLEDTDVPHKRGRKESTLSLRKLEKDAHAKLKKKARVIVEVEHEDADERQTTVH 299
           GIDEDLEDTDVPHKRGRKESTLSLRKLEKDAHAKLKKKARVIVEVEHEDADERQTTVH
Sbjct: 241 GIDEDLEDTDVPHKRGRKESTLSLRKLEKDAHAKLKKKARVIVEVEHEDADERQTTVH 298

BLAST of Moc05g09220 vs. ExPASy TrEMBL
Match: A0A6J1CUN3 (Protein MAK16 homolog OS=Momordica charantia OX=3673 GN=LOC111014743 PE=3 SV=1)

HSP 1 Score: 567.0 bits (1460), Expect = 4.6e-158
Identity = 297/298 (99.66%), Postives = 297/298 (99.66%), Query Frame = 0

Query: 1   MQHDEVIWQVIRHNHCSFMAKITTGKFCRNPYNVTGICNRSSCPLANSRYATIRDHDGVF 60
           MQHDEVIWQVIRHNHCSFMAKITTGKFCRNPYNVTGICNRSSCPLANSRYATIRDHDGVF
Sbjct: 1   MQHDEVIWQVIRHNHCSFMAKITTGKFCRNPYNVTGICNRSSCPLANSRYATIRDHDGVF 60

Query: 61  YLYMKTIERAHKPNELWERVKLPRNYEKALEIIDKHLMYWPKVLVHKTKQRLTKMTQMRI 120
           YLYMKTIERAHKPNELWERVKLPRNYEKALEIIDKHLMYWPKVLVHKTKQRLTKMTQMRI
Sbjct: 61  YLYMKTIERAHKPNELWERVKLPRNYEKALEIIDKHLMYWPKVLVHKTKQRLTKMTQMRI 120

Query: 121 RMRKLALKTREKIMTTPRKEIKREARREQKAEKAALLDKSIEKELLERLKKGVYGDIYNY 180
           RMRKLALKTREKIMTTPRKEIKREARREQKAEKAALLDKSIEKELLERLKKGVYGDIYNY
Sbjct: 121 RMRKLALKTREKIMTTPRKEIKREARREQKAEKAALLDKSIEKELLERLKKGVYGDIYNY 180

Query: 181 PVEAYNQVLEMDELQAASEEEEEEPEIEYVEGYEELEEEEDMEDFGGLAIHNPEADEDSD 240
           PVEAYNQVLEMDELQAAS EEEEEPEIEYVEGYEELEEEEDMEDFGGLAIHNPEADEDSD
Sbjct: 181 PVEAYNQVLEMDELQAAS-EEEEEPEIEYVEGYEELEEEEDMEDFGGLAIHNPEADEDSD 240

Query: 241 GIDEDLEDTDVPHKRGRKESTLSLRKLEKDAHAKLKKKARVIVEVEHEDADERQTTVH 299
           GIDEDLEDTDVPHKRGRKESTLSLRKLEKDAHAKLKKKARVIVEVEHEDADERQTTVH
Sbjct: 241 GIDEDLEDTDVPHKRGRKESTLSLRKLEKDAHAKLKKKARVIVEVEHEDADERQTTVH 297

BLAST of Moc05g09220 vs. ExPASy TrEMBL
Match: A0A6J1GW65 (Protein MAK16 homolog OS=Cucurbita moschata OX=3662 GN=LOC111458042 PE=3 SV=1)

HSP 1 Score: 547.7 bits (1410), Expect = 2.9e-152
Identity = 288/298 (96.64%), Postives = 293/298 (98.32%), Query Frame = 0

Query: 1   MQHDEVIWQVIRHNHCSFMAKITTGKFCRNPYNVTGICNRSSCPLANSRYATIRDHDGVF 60
           MQHDEVIWQVIRHNHCSFMAKI+TG FCRNPYNVTGICNRSSCPLANSRYATIRDHDGVF
Sbjct: 1   MQHDEVIWQVIRHNHCSFMAKISTGNFCRNPYNVTGICNRSSCPLANSRYATIRDHDGVF 60

Query: 61  YLYMKTIERAHKPNELWERVKLPRNYEKALEIIDKHLMYWPKVLVHKTKQRLTKMTQMRI 120
           YLYMKTIERAHKPNELWERVKLPRNYEKALEIIDKHLMYWPK+LVHKTKQRLTKMTQMRI
Sbjct: 61  YLYMKTIERAHKPNELWERVKLPRNYEKALEIIDKHLMYWPKILVHKTKQRLTKMTQMRI 120

Query: 121 RMRKLALKTREKIMTTPRKEIKREARREQKAEKAALLDKSIEKELLERLKKGVYGDIYNY 180
           RMRKLALKTREKIMTTPRKEIKREARREQKAEKAALLDKSIEKELLERLKKGVYGDIYNY
Sbjct: 121 RMRKLALKTREKIMTTPRKEIKREARREQKAEKAALLDKSIEKELLERLKKGVYGDIYNY 180

Query: 181 PVEAYNQVLEMDELQAASEEEEEEPEIEYVEGYEELEEEEDMEDFGGLAIHNPEADEDSD 240
           PVEAYN+VLEM+ELQAAS EEEEEPEIEYVEGYEELEEEEDMEDFGGLAIHNPEADEDSD
Sbjct: 181 PVEAYNEVLEMEELQAAS-EEEEEPEIEYVEGYEELEEEEDMEDFGGLAIHNPEADEDSD 240

Query: 241 GIDEDLEDTDVPHKRGRKESTLSLRKLEKDAHAKLKKKARVIVEVEHEDADERQTTVH 299
           GIDEDLED DVPHKRGRK+ST SLRKLEKDA AKLKKKARVIVEVEHEDADERQTTVH
Sbjct: 241 GIDEDLEDIDVPHKRGRKDST-SLRKLEKDARAKLKKKARVIVEVEHEDADERQTTVH 296

BLAST of Moc05g09220 vs. ExPASy TrEMBL
Match: A0A6J1I571 (Protein MAK16 homolog OS=Cucurbita maxima OX=3661 GN=LOC111470779 PE=3 SV=1)

HSP 1 Score: 547.7 bits (1410), Expect = 2.9e-152
Identity = 286/298 (95.97%), Postives = 291/298 (97.65%), Query Frame = 0

Query: 1   MQHDEVIWQVIRHNHCSFMAKITTGKFCRNPYNVTGICNRSSCPLANSRYATIRDHDGVF 60
           MQHDEVIWQVIRHNHCSFMAKITTG FCRNPYNVTGICNRSSCPLANSRYATIRDHDGVF
Sbjct: 1   MQHDEVIWQVIRHNHCSFMAKITTGNFCRNPYNVTGICNRSSCPLANSRYATIRDHDGVF 60

Query: 61  YLYMKTIERAHKPNELWERVKLPRNYEKALEIIDKHLMYWPKVLVHKTKQRLTKMTQMRI 120
           YLYMKTIERAHKPNELWERVKLPRNYEKALEIIDKHLMYWPK+LVHKTKQRLTKMTQMRI
Sbjct: 61  YLYMKTIERAHKPNELWERVKLPRNYEKALEIIDKHLMYWPKILVHKTKQRLTKMTQMRI 120

Query: 121 RMRKLALKTREKIMTTPRKEIKREARREQKAEKAALLDKSIEKELLERLKKGVYGDIYNY 180
           RMRKLALKTREKIMTTPRKEIKREARREQKAEKAALLDKSIEKELLERLKKGVYGDIYNY
Sbjct: 121 RMRKLALKTREKIMTTPRKEIKREARREQKAEKAALLDKSIEKELLERLKKGVYGDIYNY 180

Query: 181 PVEAYNQVLEMDELQAASEEEEEEPEIEYVEGYEELEEEEDMEDFGGLAIHNPEADEDSD 240
           PVEAYN+VLEM+ELQAAS EEEEEPEIEYVEGYEELEEEEDMEDFGGLAIHNPEADEDSD
Sbjct: 181 PVEAYNEVLEMEELQAAS-EEEEEPEIEYVEGYEELEEEEDMEDFGGLAIHNPEADEDSD 240

Query: 241 GIDEDLEDTDVPHKRGRKESTLSLRKLEKDAHAKLKKKARVIVEVEHEDADERQTTVH 299
           GIDEDLED DVP KRGRKEST SLRK+EKDA AKLKKKA+VIVEVEHEDADERQTT H
Sbjct: 241 GIDEDLEDIDVPRKRGRKESTYSLRKVEKDARAKLKKKAKVIVEVEHEDADERQTTAH 297

BLAST of Moc05g09220 vs. ExPASy TrEMBL
Match: A0A6J1F2I3 (Protein MAK16 homolog OS=Cucurbita moschata OX=3662 GN=LOC111439081 PE=3 SV=1)

HSP 1 Score: 545.4 bits (1404), Expect = 1.4e-151
Identity = 285/298 (95.64%), Postives = 292/298 (97.99%), Query Frame = 0

Query: 1   MQHDEVIWQVIRHNHCSFMAKITTGKFCRNPYNVTGICNRSSCPLANSRYATIRDHDGVF 60
           MQHDEVIWQVIRHNHCSFMAKI+TG FCRNPYNVTGICNRSSCPLANSRYATIRDHDGVF
Sbjct: 1   MQHDEVIWQVIRHNHCSFMAKISTGNFCRNPYNVTGICNRSSCPLANSRYATIRDHDGVF 60

Query: 61  YLYMKTIERAHKPNELWERVKLPRNYEKALEIIDKHLMYWPKVLVHKTKQRLTKMTQMRI 120
           YLYMKTIERAHKPNELWERVKLPRNYEKALEIIDKHLMYWPK+LVHKTKQRLTKMTQMRI
Sbjct: 61  YLYMKTIERAHKPNELWERVKLPRNYEKALEIIDKHLMYWPKILVHKTKQRLTKMTQMRI 120

Query: 121 RMRKLALKTREKIMTTPRKEIKREARREQKAEKAALLDKSIEKELLERLKKGVYGDIYNY 180
           RMRKLALKTREKIMTTPRKEIKREARREQKAEKAALLDKSIEKELLERLKKGVYGDIYNY
Sbjct: 121 RMRKLALKTREKIMTTPRKEIKREARREQKAEKAALLDKSIEKELLERLKKGVYGDIYNY 180

Query: 181 PVEAYNQVLEMDELQAASEEEEEEPEIEYVEGYEELEEEEDMEDFGGLAIHNPEADEDSD 240
           PVEAYN+VLEM+ELQAAS EEEEEPEIEYVEGYEELEEEEDMEDFGGLAIHNPEADEDSD
Sbjct: 181 PVEAYNEVLEMEELQAAS-EEEEEPEIEYVEGYEELEEEEDMEDFGGLAIHNPEADEDSD 240

Query: 241 GIDEDLEDTDVPHKRGRKESTLSLRKLEKDAHAKLKKKARVIVEVEHEDADERQTTVH 299
           GIDEDLED DVP KRGRKEST SLRK+EKDA AKLKKKA+VIVEVE+EDADERQTTVH
Sbjct: 241 GIDEDLEDVDVPRKRGRKESTYSLRKVEKDARAKLKKKAKVIVEVENEDADERQTTVH 297

BLAST of Moc05g09220 vs. TAIR 10
Match: AT1G23280.1 (MAK16 protein-related )

HSP 1 Score: 358.2 bits (918), Expect = 6.3e-99
Identity = 207/305 (67.87%), Postives = 240/305 (78.69%), Query Frame = 0

Query: 1   MQHDEVIWQVIRHNHCSFMAKITTGKFCRNPYNVTGICNRSSCPLANSRYATIRDHDGVF 60
           MQHDEVIWQVIRH HCS+MAKI TG FCRN YNVTGICNRSSCPLANSRYATIRDHDGVF
Sbjct: 1   MQHDEVIWQVIRHKHCSYMAKIETGIFCRNQYNVTGICNRSSCPLANSRYATIRDHDGVF 60

Query: 61  YLYMKTIERAHKPNELWERVKLPRNYEKALEIIDKHLMYWPKVLVHKTKQRLTKMTQMRI 120
           YLYMKTIERAH PN+LWERVKLP NYEKALE+IDKHL+YWPK+L HK KQRLTKMTQMRI
Sbjct: 61  YLYMKTIERAHMPNKLWERVKLPVNYEKALEMIDKHLLYWPKLLQHKVKQRLTKMTQMRI 120

Query: 121 RMRKLALKTREKIMTTPRKEIKREARREQKAEKAALLDKSIEKELLERLKKGVY-GDIYN 180
           RMRKLALKTRE ++TTPR++IKRE+RRE+KA KAA LDK+IE EL+ERLKKG+Y  +IYN
Sbjct: 121 RMRKLALKTREVVVTTPRRQIKRESRREEKAIKAAQLDKAIETELMERLKKGIYPTEIYN 180

Query: 181 YPVEAYNQVL--EMDELQAASEEEEEEPEIEYVEGYEEL--EEEEDMEDFGGLA-----I 240
                +N++L  E++      +EEEEE  IEYVEG +EL  EEEEDMEDF GL      +
Sbjct: 181 LSDSVFNKLLDREIETNDEVEKEEEEEGVIEYVEGDDELEAEEEEDMEDFSGLPSKESYL 240

Query: 241 HNPEADEDSDGIDEDLEDTDVPHKRGRKESTLSLRKLEKDAHAKLKKKARVIVEVEHEDA 296
              + D D D  D+D E+  V HK+GR     +L+K   D + K KKK RV+VEVE EDA
Sbjct: 241 EGDDHDSD-DEDDDDAEEQVVIHKKGR-----ALKK--SDDNGKAKKKPRVVVEVEQEDA 297

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022145243.11.7e-159100.00protein MAK16 homolog A isoform X1 [Momordica charantia][more]
XP_022145244.19.6e-15899.66protein MAK16 homolog isoform X2 [Momordica charantia][more]
XP_038905733.11.2e-15295.97protein MAK16 homolog isoform X2 [Benincasa hispida][more]
XP_023539083.14.6e-15295.97protein MAK16 homolog [Cucurbita pepo subsp. pepo][more]
XP_022956306.16.0e-15296.64protein MAK16 homolog [Cucurbita moschata] >XP_022956307.1 protein MAK16 homolog... [more]
Match NameE-valueIdentityDescription
Q66L332.7e-6247.71Protein MAK16 homolog A OS=Xenopus laevis OX=8355 GN=mak16-a PE=2 SV=1[more]
Q6NYD43.0e-6147.99Protein MAK16 homolog OS=Danio rerio OX=7955 GN=mak16 PE=2 SV=1[more]
Q7ZYG55.1e-6148.52Protein MAK16 homolog B OS=Xenopus laevis OX=8355 GN=mak16-b PE=2 SV=1[more]
Q6P7N18.7e-6148.85Protein MAK16 homolog OS=Xenopus tropicalis OX=8364 GN=mak16 PE=2 SV=1[more]
Q8BGS03.6e-5947.23Protein MAK16 homolog OS=Mus musculus OX=10090 GN=Mak16 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1CVF08.5e-160100.00Protein MAK16 homolog OS=Momordica charantia OX=3673 GN=LOC111014743 PE=3 SV=1[more]
A0A6J1CUN34.6e-15899.66Protein MAK16 homolog OS=Momordica charantia OX=3673 GN=LOC111014743 PE=3 SV=1[more]
A0A6J1GW652.9e-15296.64Protein MAK16 homolog OS=Cucurbita moschata OX=3662 GN=LOC111458042 PE=3 SV=1[more]
A0A6J1I5712.9e-15295.97Protein MAK16 homolog OS=Cucurbita maxima OX=3661 GN=LOC111470779 PE=3 SV=1[more]
A0A6J1F2I31.4e-15195.64Protein MAK16 homolog OS=Cucurbita moschata OX=3662 GN=LOC111439081 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT1G23280.16.3e-9967.87MAK16 protein-related [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (OHB3-1) v2
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 182..202
NoneNo IPR availableGENE3D3.30.390.110coord: 3..128
e-value: 2.8E-28
score: 100.7
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 247..263
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 229..263
NoneNo IPR availablePANTHERPTHR23405:SF7PROTEIN MAK16 HOMOLOGcoord: 1..295
NoneNo IPR availablePANTHERPTHR23405MAINTENANCE OF KILLER 16 MAK16 PROTEIN-RELATEDcoord: 1..295
IPR006958Mak16 proteinPIRSFPIRSF003352MAK16coord: 1..298
e-value: 5.6E-108
score: 358.6
IPR006958Mak16 proteinPFAMPF04874Mak16coord: 138..224
e-value: 6.2E-23
score: 81.3
IPR029004Ribosomal L28e/Mak16PFAMPF01778Ribosomal_L28ecoord: 6..117
e-value: 9.9E-39
score: 132.6

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Moc05g09220.1Moc05g09220.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0000460 maturation of 5.8S rRNA
biological_process GO:0000470 maturation of LSU-rRNA
cellular_component GO:0005730 nucleolus
cellular_component GO:0030687 preribosome, large subunit precursor