Moc05g08520 (gene) Bitter gourd (OHB3-1) v2

Overview
NameMoc05g08520
Typegene
OrganismMomordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
DescriptionHXXXD-type acyl-transferase family protein
Locationchr5: 6509300 .. 6510703 (+)
RNA-Seq ExpressionMoc05g08520
SyntenyMoc05g08520
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGAGGTGAAAATTTTGTCAAGAGAAACTATTATGCCCTCTTCCCCAACTCCCCCTCACCTTAAAACCTTCGAACTTTCACTTATCGACCAATTGTCTCCTCCACTTTATATTCCTCTTTTATTTTTCTACCCCATCAAAAACAAACATGATCTCGAGACCCACAACCAAAAGAAAGCAATCGCAACACTGAAAAATTCTCTATCAAAAACTCTAACTAATTTTTTCTTGTTGGCAGGAAGAATCACCAACACGTCCATTCGTTGTAGCAACGACGAAGGAGCTGTCTTTATCGAAGCAACCGTTAACTGCAACATTTCCGACATCCTCAAAGAACCAAACAATGAGGTTTTAACGAAACTCCTCCCGTGTTCTATTCAATGCACTAAACCAACAGAAGAATATGCTCAGATTGTTGCTCAAGCCAACATCTTCAACTGTGGTGGAATTGTGATCTCTCTTTGTTTGTTGCACAAGCTCATGGATGGGATAAGTTTTAGTTGTTTTCTCAAATCTTGGGCATCCATTAATAAAGGGTCTTTGGATAATTCTTCTCAAAATTTGGTATGTTCCAACTACAAAACATTCTCCTCGCTCTTTCCCCAAACAAACTTATTGTCTTTTCATCACCAAATACACGAAGGAGTAGACGACATATGCTCGAGTTCCAAACCGAACCGTTGTTTGCAACGGTTCGTGTTCGGAGCGAAAGCAATATCAGATCTAAAAGCGAAAGCAAAATCCACTGAGGTTCCAAATCCAACCAGTGTTGAAGCATTAACTGGATTTATTTGGAATTGTGCCATGAAATTGAAAGCTACATCTATGGGAAATGATGATGATTCTCAAAGGCCATCCACTTTGTCACATGTGGTGAACTTGAGAAAGAGAATGGAGCCGCCATTGCCTGAAATCTCAATAGGGAACGTTTTGTGGGGTATGGTTGCTCATTATTGTTCATCAAATTATGAAACTCAAGTGGAGCTAAGGGATTTAGTGGTTTTGTTGAGACAATCATTTTCAAAAATCAACAATGATTTCATAAAAGGGTTGATGGGAAATGACGGAAATAGAGCTGTTTTGGAGTTGCTTGGTGAGATAAATAAGTTTCCATCTAAAGTTCCAAATTATTACTTTTTCACAAGTTGGAGAAATATGGATTTGAATGAGGTCGATTTTGGATGGGGGAAGCCTATATGGATTGGCATTGCGGGGAATTCAAATGTTATAACCAAGGATTTTATTCTTTTGTTGGATACCCCATCGGGCGATGGAATAGAGGCATGGATGGTACTCGATCAAAAGAAAATGAAACTTTTACAACAAAATCCACAATTTCTCGAGTTTGCATCGTTGAATCCGAGTATCTCTATAAAAGGCGAGGTTGAAGATTTGAATCTCTAA

mRNA sequence

ATGGAGGTGAAAATTTTGTCAAGAGAAACTATTATGCCCTCTTCCCCAACTCCCCCTCACCTTAAAACCTTCGAACTTTCACTTATCGACCAATTGTCTCCTCCACTTTATATTCCTCTTTTATTTTTCTACCCCATCAAAAACAAACATGATCTCGAGACCCACAACCAAAAGAAAGCAATCGCAACACTGAAAAATTCTCTATCAAAAACTCTAACTAATTTTTTCTTGTTGGCAGGAAGAATCACCAACACGTCCATTCGTTGTAGCAACGACGAAGGAGCTGTCTTTATCGAAGCAACCGTTAACTGCAACATTTCCGACATCCTCAAAGAACCAAACAATGAGGTTTTAACGAAACTCCTCCCGTGTTCTATTCAATGCACTAAACCAACAGAAGAATATGCTCAGATTGTTGCTCAAGCCAACATCTTCAACTGTGGTGGAATTGTGATCTCTCTTTGTTTGTTGCACAAGCTCATGGATGGGATAAGTTTTAGTTGTTTTCTCAAATCTTGGGCATCCATTAATAAAGGGTCTTTGGATAATTCTTCTCAAAATTTGGTATGTTCCAACTACAAAACATTCTCCTCGCTCTTTCCCCAAACAAACTTATTGTCTTTTCATCACCAAATACACGAAGGAGTAGACGACATATGCTCGAGTTCCAAACCGAACCGTTGTTTGCAACGGTTCGTGTTCGGAGCGAAAGCAATATCAGATCTAAAAGCGAAAGCAAAATCCACTGAGGTTCCAAATCCAACCAGTGTTGAAGCATTAACTGGATTTATTTGGAATTGTGCCATGAAATTGAAAGCTACATCTATGGGAAATGATGATGATTCTCAAAGGCCATCCACTTTGTCACATGTGGTGAACTTGAGAAAGAGAATGGAGCCGCCATTGCCTGAAATCTCAATAGGGAACGTTTTGTGGGGTATGGTTGCTCATTATTGTTCATCAAATTATGAAACTCAAGTGGAGCTAAGGGATTTAGTGGTTTTGTTGAGACAATCATTTTCAAAAATCAACAATGATTTCATAAAAGGGTTGATGGGAAATGACGGAAATAGAGCTGTTTTGGAGTTGCTTGGTGAGATAAATAAGTTTCCATCTAAAGTTCCAAATTATTACTTTTTCACAAGTTGGAGAAATATGGATTTGAATGAGGTCGATTTTGGATGGGGGAAGCCTATATGGATTGGCATTGCGGGGAATTCAAATGTTATAACCAAGGATTTTATTCTTTTGTTGGATACCCCATCGGGCGATGGAATAGAGGCATGGATGGTACTCGATCAAAAGAAAATGAAACTTTTACAACAAAATCCACAATTTCTCGAGTTTGCATCGTTGAATCCGAGTATCTCTATAAAAGGCGAGGTTGAAGATTTGAATCTCTAA

Coding sequence (CDS)

ATGGAGGTGAAAATTTTGTCAAGAGAAACTATTATGCCCTCTTCCCCAACTCCCCCTCACCTTAAAACCTTCGAACTTTCACTTATCGACCAATTGTCTCCTCCACTTTATATTCCTCTTTTATTTTTCTACCCCATCAAAAACAAACATGATCTCGAGACCCACAACCAAAAGAAAGCAATCGCAACACTGAAAAATTCTCTATCAAAAACTCTAACTAATTTTTTCTTGTTGGCAGGAAGAATCACCAACACGTCCATTCGTTGTAGCAACGACGAAGGAGCTGTCTTTATCGAAGCAACCGTTAACTGCAACATTTCCGACATCCTCAAAGAACCAAACAATGAGGTTTTAACGAAACTCCTCCCGTGTTCTATTCAATGCACTAAACCAACAGAAGAATATGCTCAGATTGTTGCTCAAGCCAACATCTTCAACTGTGGTGGAATTGTGATCTCTCTTTGTTTGTTGCACAAGCTCATGGATGGGATAAGTTTTAGTTGTTTTCTCAAATCTTGGGCATCCATTAATAAAGGGTCTTTGGATAATTCTTCTCAAAATTTGGTATGTTCCAACTACAAAACATTCTCCTCGCTCTTTCCCCAAACAAACTTATTGTCTTTTCATCACCAAATACACGAAGGAGTAGACGACATATGCTCGAGTTCCAAACCGAACCGTTGTTTGCAACGGTTCGTGTTCGGAGCGAAAGCAATATCAGATCTAAAAGCGAAAGCAAAATCCACTGAGGTTCCAAATCCAACCAGTGTTGAAGCATTAACTGGATTTATTTGGAATTGTGCCATGAAATTGAAAGCTACATCTATGGGAAATGATGATGATTCTCAAAGGCCATCCACTTTGTCACATGTGGTGAACTTGAGAAAGAGAATGGAGCCGCCATTGCCTGAAATCTCAATAGGGAACGTTTTGTGGGGTATGGTTGCTCATTATTGTTCATCAAATTATGAAACTCAAGTGGAGCTAAGGGATTTAGTGGTTTTGTTGAGACAATCATTTTCAAAAATCAACAATGATTTCATAAAAGGGTTGATGGGAAATGACGGAAATAGAGCTGTTTTGGAGTTGCTTGGTGAGATAAATAAGTTTCCATCTAAAGTTCCAAATTATTACTTTTTCACAAGTTGGAGAAATATGGATTTGAATGAGGTCGATTTTGGATGGGGGAAGCCTATATGGATTGGCATTGCGGGGAATTCAAATGTTATAACCAAGGATTTTATTCTTTTGTTGGATACCCCATCGGGCGATGGAATAGAGGCATGGATGGTACTCGATCAAAAGAAAATGAAACTTTTACAACAAAATCCACAATTTCTCGAGTTTGCATCGTTGAATCCGAGTATCTCTATAAAAGGCGAGGTTGAAGATTTGAATCTCTAA

Protein sequence

MEVKILSRETIMPSSPTPPHLKTFELSLIDQLSPPLYIPLLFFYPIKNKHDLETHNQKKAIATLKNSLSKTLTNFFLLAGRITNTSIRCSNDEGAVFIEATVNCNISDILKEPNNEVLTKLLPCSIQCTKPTEEYAQIVAQANIFNCGGIVISLCLLHKLMDGISFSCFLKSWASINKGSLDNSSQNLVCSNYKTFSSLFPQTNLLSFHHQIHEGVDDICSSSKPNRCLQRFVFGAKAISDLKAKAKSTEVPNPTSVEALTGFIWNCAMKLKATSMGNDDDSQRPSTLSHVVNLRKRMEPPLPEISIGNVLWGMVAHYCSSNYETQVELRDLVVLLRQSFSKINNDFIKGLMGNDGNRAVLELLGEINKFPSKVPNYYFFTSWRNMDLNEVDFGWGKPIWIGIAGNSNVITKDFILLLDTPSGDGIEAWMVLDQKKMKLLQQNPQFLEFASLNPSISIKGEVEDLNL
Homology
BLAST of Moc05g08520 vs. NCBI nr
Match: XP_022138192.1 (BAHD acyltransferase BIA1-like [Momordica charantia])

HSP 1 Score: 947.2 bits (2447), Expect = 5.3e-272
Identity = 467/467 (100.00%), Postives = 467/467 (100.00%), Query Frame = 0

Query: 1   MEVKILSRETIMPSSPTPPHLKTFELSLIDQLSPPLYIPLLFFYPIKNKHDLETHNQKKA 60
           MEVKILSRETIMPSSPTPPHLKTFELSLIDQLSPPLYIPLLFFYPIKNKHDLETHNQKKA
Sbjct: 1   MEVKILSRETIMPSSPTPPHLKTFELSLIDQLSPPLYIPLLFFYPIKNKHDLETHNQKKA 60

Query: 61  IATLKNSLSKTLTNFFLLAGRITNTSIRCSNDEGAVFIEATVNCNISDILKEPNNEVLTK 120
           IATLKNSLSKTLTNFFLLAGRITNTSIRCSNDEGAVFIEATVNCNISDILKEPNNEVLTK
Sbjct: 61  IATLKNSLSKTLTNFFLLAGRITNTSIRCSNDEGAVFIEATVNCNISDILKEPNNEVLTK 120

Query: 121 LLPCSIQCTKPTEEYAQIVAQANIFNCGGIVISLCLLHKLMDGISFSCFLKSWASINKGS 180
           LLPCSIQCTKPTEEYAQIVAQANIFNCGGIVISLCLLHKLMDGISFSCFLKSWASINKGS
Sbjct: 121 LLPCSIQCTKPTEEYAQIVAQANIFNCGGIVISLCLLHKLMDGISFSCFLKSWASINKGS 180

Query: 181 LDNSSQNLVCSNYKTFSSLFPQTNLLSFHHQIHEGVDDICSSSKPNRCLQRFVFGAKAIS 240
           LDNSSQNLVCSNYKTFSSLFPQTNLLSFHHQIHEGVDDICSSSKPNRCLQRFVFGAKAIS
Sbjct: 181 LDNSSQNLVCSNYKTFSSLFPQTNLLSFHHQIHEGVDDICSSSKPNRCLQRFVFGAKAIS 240

Query: 241 DLKAKAKSTEVPNPTSVEALTGFIWNCAMKLKATSMGNDDDSQRPSTLSHVVNLRKRMEP 300
           DLKAKAKSTEVPNPTSVEALTGFIWNCAMKLKATSMGNDDDSQRPSTLSHVVNLRKRMEP
Sbjct: 241 DLKAKAKSTEVPNPTSVEALTGFIWNCAMKLKATSMGNDDDSQRPSTLSHVVNLRKRMEP 300

Query: 301 PLPEISIGNVLWGMVAHYCSSNYETQVELRDLVVLLRQSFSKINNDFIKGLMGNDGNRAV 360
           PLPEISIGNVLWGMVAHYCSSNYETQVELRDLVVLLRQSFSKINNDFIKGLMGNDGNRAV
Sbjct: 301 PLPEISIGNVLWGMVAHYCSSNYETQVELRDLVVLLRQSFSKINNDFIKGLMGNDGNRAV 360

Query: 361 LELLGEINKFPSKVPNYYFFTSWRNMDLNEVDFGWGKPIWIGIAGNSNVITKDFILLLDT 420
           LELLGEINKFPSKVPNYYFFTSWRNMDLNEVDFGWGKPIWIGIAGNSNVITKDFILLLDT
Sbjct: 361 LELLGEINKFPSKVPNYYFFTSWRNMDLNEVDFGWGKPIWIGIAGNSNVITKDFILLLDT 420

Query: 421 PSGDGIEAWMVLDQKKMKLLQQNPQFLEFASLNPSISIKGEVEDLNL 468
           PSGDGIEAWMVLDQKKMKLLQQNPQFLEFASLNPSISIKGEVEDLNL
Sbjct: 421 PSGDGIEAWMVLDQKKMKLLQQNPQFLEFASLNPSISIKGEVEDLNL 467

BLAST of Moc05g08520 vs. NCBI nr
Match: XP_038875765.1 (BAHD acyltransferase BIA1-like [Benincasa hispida])

HSP 1 Score: 555.8 bits (1431), Expect = 3.5e-154
Identity = 296/471 (62.85%), Postives = 367/471 (77.92%), Query Frame = 0

Query: 1   MEVKILSRETIMPSSPTPPHLKTFELSLIDQLSPPLYIPLLFFYPIKNKHDLETHNQKKA 60
           MEVK+LSRETI+PSSPTPPHL+TF+LSL+DQLSP LYIPLLFFYP+K  +D++  + KK 
Sbjct: 1   MEVKVLSRETIIPSSPTPPHLQTFQLSLLDQLSPMLYIPLLFFYPMKTNYDIDHQDPKKT 60

Query: 61  IATLKNSLSKTLTNFFLLAGRITNTSIRCSNDEGAVFIEATVNCNISDILKEPNNEVLTK 120
           IATLKNSLSKTL+ F+LLAGRIT+ SI C ND+GAVF EATV+CN+ +ILKEPN+EVL K
Sbjct: 61  IATLKNSLSKTLSRFYLLAGRITDKSIHC-NDKGAVFQEATVSCNMFEILKEPNSEVLAK 120

Query: 121 LLPCSIQC-TKPTEEYAQIVAQANIFNCGGIVISLCLLHKLMDGISFSCFLKSWASINKG 180
           LLPCS+ C TKP EEY QIV QANIF CGGI ISLCLLHKL+D  +FSCFL+SWA+ N+ 
Sbjct: 121 LLPCSLSCTTKPIEEYTQIVVQANIFKCGGIAISLCLLHKLIDAATFSCFLRSWATTNRE 180

Query: 181 ---SLDNS-SQNLVCSNYKTFSSLFPQTNLLSFHHQIHEGVDDICSSSKPNRCLQRFVFG 240
              S D+S + N+ C +Y +FSSLFPQTNLL F    H+    + ++      LQRFVF 
Sbjct: 181 LLLSFDSSPNNNMACYDYNSFSSLFPQTNLLPF----HKAAVTLSTTPARKHHLQRFVFR 240

Query: 241 AKAISDLKAKAKSTEVPNPTSVEALTGFIWNCAMKLKATSMGNDDDSQRPSTLSHVVNLR 300
           +KAI DLKAKAKS++VPNPT VE LTGFIW  AMK     + + D+SQ+ STLSHVVN+R
Sbjct: 241 SKAILDLKAKAKSSDVPNPTCVETLTGFIWKYAMK-----VADHDNSQKQSTLSHVVNMR 300

Query: 301 KRMEPPLPEISIGNVLWGMVAHYCSSNYETQ---VELRDLVVLLRQSFSKINN-DFIKGL 360
           K +EP LPE S+GN+LWG VAHY S+    +   +EL  LV LLRQSF K+NN ++IK L
Sbjct: 301 KIIEPSLPEDSVGNILWGTVAHYYSTTSHEEMEDLELSKLVSLLRQSFKKVNNKEYIKEL 360

Query: 361 -MGNDGNRA--VLELLGEINKFPSKVPNYYFFTSWRNMDLNEVDFGWGKPIWIGIAGNSN 420
            M +D  R+  V++L+GEINK P  + NYYFFTSW+NM+LNEVDFGWGKP+W  IAG+SN
Sbjct: 361 IMESDKGRSDGVMKLVGEINKCP--LSNYYFFTSWKNMNLNEVDFGWGKPLWFAIAGDSN 420

Query: 421 VITK-DFILLLDTPSGDG-IEAWMVLDQKKMKLLQQNPQFLEFASLNPSIS 458
            + K +F++LLD+   DG +EAW++LD+KKM+LL QN QFLEFA LNPSI+
Sbjct: 421 EMMKGNFVILLDSVLDDGSMEAWILLDEKKMQLLDQNQQFLEFALLNPSIN 459

BLAST of Moc05g08520 vs. NCBI nr
Match: XP_004152093.1 (stemmadenine O-acetyltransferase [Cucumis sativus])

HSP 1 Score: 555.1 bits (1429), Expect = 5.9e-154
Identity = 295/477 (61.84%), Postives = 365/477 (76.52%), Query Frame = 0

Query: 1   MEVKILSRETIMPSSPTPPHLKTFELSLIDQLSPPLYIPLLFFYPIKNKHD-LETHNQ-- 60
           MEVK+LS+ETI+PSSPTPPHL+TF+LSL+DQLSP LYIPLL FYP+K  +D ++ H+Q  
Sbjct: 1   MEVKVLSKETIIPSSPTPPHLQTFQLSLLDQLSPMLYIPLLLFYPMKRSYDHIDQHDQDP 60

Query: 61  KKAIATLKNSLSKTLTNFFLLAGRITNTSIRCSNDEGAVFIEATVNCNISDILKEPNNEV 120
           KK IATLK SLSKTL+ F+LLAGRI + SI C ND+GAVF+EAT+N N+ DILKEPNNEV
Sbjct: 61  KKTIATLKTSLSKTLSRFYLLAGRIIDKSIHC-NDKGAVFMEATINTNMFDILKEPNNEV 120

Query: 121 LTKLLPCSIQC-TKPTEEYAQIVAQANIFNCGGIVISLCLLHKLMDGISFSCFLKSWASI 180
           LTKLLPCS+ C TKP EEY QIV QAN+F CGGI ISLCLLHKL+D  +F CFL+SWA+ 
Sbjct: 121 LTKLLPCSLLCNTKPIEEYPQIVVQANVFKCGGIAISLCLLHKLIDAATFCCFLRSWATT 180

Query: 181 NK---GSLDNSS-QNLVCSNYKTFSSLFPQTNLLSFHHQIHEGVDDICSSS---KPNRCL 240
           N+     LD+SS  N+VC +YK+FSSLFPQTNLL FH ++    + +   S      R L
Sbjct: 181 NRELLSQLDHSSPNNMVCFDYKSFSSLFPQTNLLPFHQRLINNDNAVILPSSIFNGKRRL 240

Query: 241 QRFVFGAKAISDLKAKAKSTEVPNPTSVEALTGFIWNCAMKLKATSMGNDDDSQRPSTLS 300
           QRFVF +KAI DLKAKAKS ++PNPT VEA+T FIW   MK     + +  DSQ PSTLS
Sbjct: 241 QRFVFRSKAILDLKAKAKSCDIPNPTCVEAITCFIWKYLMK-----VADGGDSQMPSTLS 300

Query: 301 HVVNLRKRMEPPLPEISIGNVLWGMVAHYCSSNYETQ----VELRDLVVLLRQSFSKINN 360
           HVVN+RK +EP L E+S+GN++WG VAH+ S+    +    +EL  LV LLRQS  KIN 
Sbjct: 301 HVVNIRKMIEPSLGEVSLGNIMWGTVAHHFSTTRPNESFEGLELSKLVSLLRQSLKKINK 360

Query: 361 DFIKGL-MGNDGNR--AVLELLGEINKFPSKVPNYYFFTSWRNMDLNEVDFGWGKPIWIG 420
           D+IK L MG D  R   V++L+GEINK+P  + NYYFFTSW+NM LNE+DFGWGKP+W G
Sbjct: 361 DYIKELIMGGDKGRRNGVMKLVGEINKWP--ISNYYFFTSWKNMKLNELDFGWGKPLWFG 420

Query: 421 IAGNSNVITKDFILLLDTPSGDG-IEAWMVLDQKKMKLLQQNPQFLEFASLNPSISI 459
           IAG+SN +  + I+L+D  S DG IEAW++LD+K+M+LL+QNPQFLEFA LNPSI +
Sbjct: 421 IAGDSNEMMGNIIVLVDNVSDDGSIEAWILLDEKEMQLLEQNPQFLEFALLNPSIHL 469

BLAST of Moc05g08520 vs. NCBI nr
Match: KAE8649121.1 (hypothetical protein Csa_015082 [Cucumis sativus])

HSP 1 Score: 553.5 bits (1425), Expect = 1.7e-153
Identity = 294/479 (61.38%), Postives = 364/479 (75.99%), Query Frame = 0

Query: 1   MEVKILSRETIMPSSPTPPHLKTFELSLIDQLSPPLYIPLLFFYPIKNKHD-LETHNQ-- 60
           MEVK+LS+ETI+PSSPTPPHL+TF+LSL+DQLSP LYIPLL FYP+K  +D ++ H+Q  
Sbjct: 1   MEVKVLSKETIIPSSPTPPHLQTFQLSLLDQLSPMLYIPLLLFYPMKRSYDHIDQHDQDP 60

Query: 61  KKAIATLKNSLSKTLTNFFLLAGRITNTSIRCSNDEGAVFIEATVNCNISDILKEPNNEV 120
           KK IATLK SLSKTL+ F+LLAGRI + SI C ND+GAVF+EAT+N N+ DILKEPNNEV
Sbjct: 61  KKTIATLKTSLSKTLSRFYLLAGRIIDKSIHC-NDKGAVFMEATINTNMFDILKEPNNEV 120

Query: 121 LTKLLPCSIQC-TKPTEEYAQIVAQANIFNCGGIVISLCLLHKLMDGISFSCFLKSWASI 180
           LTKLLPCS+ C TKP EEY QIV QAN+F CGGI ISLCLLHKL+D  +F CFL+SWA+ 
Sbjct: 121 LTKLLPCSLLCNTKPIEEYPQIVVQANVFKCGGIAISLCLLHKLIDAATFCCFLRSWATT 180

Query: 181 NK---GSLDNSS-QNLVCSNYKTFSSLFPQTNLLSFHHQIHEGVDDICSSS---KPNRCL 240
           N+     LD+SS  N+VC +YK+FSSLFPQTNLL FH ++    + +   S      R L
Sbjct: 181 NRELLSQLDHSSPNNMVCFDYKSFSSLFPQTNLLPFHQRLINNDNAVILPSSIFNGKRRL 240

Query: 241 QRFVFGAKAISDLKAKAKSTEVPNPTSVEALTGFIWNCAMKLKATSMGNDDDSQRPSTLS 300
           QRFVF +KAI DLKAKAKS ++PNPT VEA+T FIW   MK     + +  DSQ PSTLS
Sbjct: 241 QRFVFRSKAILDLKAKAKSCDIPNPTCVEAITCFIWKYLMK-----VADGGDSQMPSTLS 300

Query: 301 HVVNLRKRMEPPLPEISIGNVLWGMVAHYCSSNYETQ----VELRDLVVLLRQSFSKINN 360
           HVVN+RK +EP L E+S+GN++WG VAH+ S+    +    +EL  LV LLRQS  KIN 
Sbjct: 301 HVVNIRKMIEPSLGEVSLGNIMWGTVAHHFSTTRPNESFEGLELSKLVSLLRQSLKKINK 360

Query: 361 DFIKGL-MGNDGNR--AVLELLGEINKFPSKVPNYYFFTSWRNMDLNEVDFGWGKPIWIG 420
           D+IK L MG D  R   V++L+GEINK+P  + NYYFFTSW+NM LNE+DFGWGKP+W G
Sbjct: 361 DYIKELIMGGDKGRRNGVMKLVGEINKWP--ISNYYFFTSWKNMKLNELDFGWGKPLWFG 420

Query: 421 IAGNSNVITKDFILLLDTPSGDG-IEAWMVLDQKKMKLLQQNPQFLEFASLNPSISIKG 461
           IAG+SN +  + I+L+D  S DG IEAW++LD+K+M+LL+QNPQFLEFA LNP   + G
Sbjct: 421 IAGDSNEMMGNIIVLVDNVSDDGSIEAWILLDEKEMQLLEQNPQFLEFALLNPRFCVWG 471

BLAST of Moc05g08520 vs. NCBI nr
Match: XP_008453950.1 (PREDICTED: salutaridinol 7-O-acetyltransferase-like [Cucumis melo] >TYK16967.1 salutaridinol 7-O-acetyltransferase-like [Cucumis melo var. makuwa])

HSP 1 Score: 551.6 bits (1420), Expect = 6.5e-153
Identity = 277/484 (57.23%), Postives = 355/484 (73.35%), Query Frame = 0

Query: 1   MEVKILSRETIMPSSPTPPHLKTFELSLIDQLSPPLYIPLLFFYPIKNKHDLETH-NQKK 60
           MEVK+LS+E +MPSSPTPPHLKTF+LSL+DQ SP LY PL+ FYP+ NK D   H + ++
Sbjct: 1   MEVKVLSKENVMPSSPTPPHLKTFQLSLLDQFSPVLYAPLIIFYPMDNKDDPRHHQSHEE 60

Query: 61  AIATLKNSLSKTLTNFFLLAGRITNTSIRCSNDEGAVFIEATVNCNISDILKEPNNEVLT 120
            +ATLK+SLSKTL++F+LLAGRI + SIRCS DEGAVFIEATV+C++S+ILK+PNNE L 
Sbjct: 61  LMATLKSSLSKTLSHFYLLAGRIVDKSIRCS-DEGAVFIEATVSCSMSEILKQPNNEFLM 120

Query: 121 KLLPCSIQCTKPTEEYAQIVAQANIFNCGGIVISLCLLHKLMDGISFSCFLKSWASINKG 180
           KL+PCS +CTKP EEYA ++ QAN+F+CGGI ISLCLLHKLMD  +  CFLK WA+IN+ 
Sbjct: 121 KLIPCSERCTKPIEEYAHVIVQANVFDCGGIAISLCLLHKLMDATTIGCFLKCWATINRR 180

Query: 181 S-------LDNSSQNL------------------VCSNYKTFSSLFPQTNLLSFHHQIHE 240
           S       +DN +  L                  VC +YK  SSLFPQTN L FH ++HE
Sbjct: 181 SFTTTNLTIDNGASELLTSSSMTKDDNSDPFSKMVCYDYKELSSLFPQTNFLPFHPRLHE 240

Query: 241 GVDDICSSSKPNRCLQRFVFGAKAISDLKAKAKSTEVPNPTSVEALTGFIWNCAMKLKAT 300
            +   C         QRFVF  KAI DLKAKAKS +VPNPTSVE L+GFIW CA++  +T
Sbjct: 241 ALSSNCEEKS---SFQRFVFKEKAILDLKAKAKSNDVPNPTSVEVLSGFIWKCALEAAST 300

Query: 301 SMGNDDDSQRPSTLSHVVNLRKRMEPPLPEISIGNVLWGMVAHYCSSNYETQVELRDLVV 360
            +G    SQ PS L+H VNLRKRMEPPLPE S+GN+ W +VAHY +   +TQVEL +LV 
Sbjct: 301 KLGL---SQIPSILTHAVNLRKRMEPPLPEFSVGNIFWNVVAHYLADK-KTQVELSELVS 360

Query: 361 LLRQSFSKINNDFIKGLMGNDGNRAVLELLGEINKFPSKVPNYYFFTSWRNMDLNEVDFG 420
           L+RQSF  IN ++IK ++GN+G+ A+++L+ E N    ++P  Y  TSWRNMDL+EV+FG
Sbjct: 361 LIRQSFVDINGNYIKKIVGNEGSEALVKLVWERNMKLFQIPKLYICTSWRNMDLSEVNFG 420

Query: 421 WGKPIWIGIAGNSNVITKDFILLLDTPSGDGIEAWMVLDQKKMKLLQQNPQFLEFASLNP 459
           WGKPIWIG AGNSN   K+ I+LLD  S DG+EAW++L++++M++L +N +FL FA LNP
Sbjct: 421 WGKPIWIGSAGNSNTTIKNMIVLLDAISNDGVEAWVILEEEEMQILLENQEFLNFALLNP 476

BLAST of Moc05g08520 vs. ExPASy Swiss-Prot
Match: Q94FT4 (Salutaridinol 7-O-acetyltransferase OS=Papaver somniferum OX=3469 GN=SALAT PE=1 SV=1)

HSP 1 Score: 201.8 bits (512), Expect = 1.7e-50
Identity = 146/465 (31.40%), Postives = 234/465 (50.32%), Query Frame = 0

Query: 3   VKILSRETIMPSSPTPPHLKTFELSLIDQLSP-PLYIPLLFFYPIKNKHDLETHNQKKAI 62
           V+++S+ETI P++PTP  LK F LSL+DQ  P   Y+P++ FYP    +   + N    +
Sbjct: 9   VEVISKETIKPTTPTPSQLKNFNLSLLDQCFPLYYYVPIILFYPATAANSTGSSNHHDDL 68

Query: 63  ATLKNSLSKTLTNFFLLAGRITNTSIRCSNDEGAVFIEATVNCNISDILKEPNNEVLTKL 122
             LK+SLSKTL +F+ +AGR+ +  +   +D+G  F +  +   + + + +P +  L++L
Sbjct: 69  DLLKSSLSKTLVHFYPMAGRMIDNILVDCHDQGINFYKVKIRGKMCEFMSQP-DVPLSQL 128

Query: 123 LPCSIQCTKPTEEYAQIVAQANIFNCGGIVISLCLLHKLMDGISFSCFLKSWASINKGSL 182
           LP  +      +E A ++ Q N+F+CGG  I   + HK+ D  + S F++SWAS  K S 
Sbjct: 129 LPSEVVSASVPKE-ALVIVQVNMFDCGGTAICSSVSHKIADAATMSTFIRSWASTTKTSR 188

Query: 183 DNSSQNLVCSN--YKTF--SSLFPQTNLLSFHHQIHEGVDDICSSSKP------NRCLQR 242
              S   V       +F  +SLFP +  L+       G+ +I  SS P          +R
Sbjct: 189 SGGSTAAVTDQKLIPSFDSASLFPPSERLT----SPSGMSEIPFSSTPEDTEDDKTVSKR 248

Query: 243 FVFGAKAISDLKAKAKSTEVPN-----PTSVEALTGFIWNCAMKLKATSMGNDDDSQRPS 302
           FVF    I+ ++ K +     N      T VE +T  IW   M  K+T  G         
Sbjct: 249 FVFDFAKITSVREKLQVLMHDNYKSRRQTRVEVVTSLIWKSVM--KSTPAG------FLP 308

Query: 303 TLSHVVNLRKRMEPPLPEISIGNVLWGMVAH---------------YCSSNYETQV---E 362
            + H VNLRK+M+PPL ++S GN+   + A                  S++ E+QV   E
Sbjct: 309 VVHHAVNLRKKMDPPLQDVSFGNLSVTVSAFLPATTTTTTNAVNKTINSTSSESQVVLHE 368

Query: 363 LRDLVVLLRQSFSKINND--FIKGLMGN--DGNRAVLELLGEINKFPSKVPNYYFFTSWR 422
           L D +  +R    K+  D   ++ ++ N   G+ A ++ + ++     +V N++  +SW 
Sbjct: 369 LHDFIAQMRSEIDKVKGDKGSLEKVIQNFASGHDASIKKINDV-----EVINFW-ISSWC 428

Query: 423 NMDLNEVDFGWGKPIWIGIAGNSNVITKDFILLLDTPSGDGIEAW 430
            M L E+DFGWGKPIW+ +  N     K+   + DT  G+GIE W
Sbjct: 429 RMGLYEIDFGWGKPIWVTVDPNIKP-NKNCFFMNDTKCGEGIEVW 452

BLAST of Moc05g08520 vs. ExPASy Swiss-Prot
Match: O23393 (BAHD acyltransferase BIA1 OS=Arabidopsis thaliana OX=3702 GN=BIA1 PE=2 SV=1)

HSP 1 Score: 200.3 bits (508), Expect = 4.8e-50
Identity = 136/466 (29.18%), Postives = 231/466 (49.57%), Query Frame = 0

Query: 2   EVKILSRETIMPSSPTPPHLKTFELSLIDQLSPPLYIPLLFFYPIKNKHDLETHNQKKAI 61
           ++++  +E I P+SP+P      +LS++D   P +Y+  +FFY      DL T + +   
Sbjct: 4   KLEVTGKEVIKPASPSP--RDRLQLSILDLYCPGIYVSTIFFY------DLITESSEVFS 63

Query: 62  ATLKNSLSKTLTNFFLLAGRITNTSIRCSNDEGAVFIEATVNCNISDILKEPNNEVLTKL 121
             LK SLS+TL+ F+ LAGRI   SI C NDEGAVF EA  +  + D L+  N + L+  
Sbjct: 64  ENLKLSLSETLSRFYPLAGRIEGLSISC-NDEGAVFTEARTDLLLPDFLRNLNTDSLSGF 123

Query: 122 LPCSIQCTKPTEEYAQIVAQANIFNCG-GIVISLCLLHKLMDGISFSCFLKSWASINKGS 181
           LP ++   +    +  +  +   F  G G+ +S+ + HK+ D  S   F+K WA+     
Sbjct: 124 LP-TLAAGESPAAWPLLSVKVTFFGSGSGVAVSVSVSHKICDIASLVTFVKDWATTTAKG 183

Query: 182 LDNSSQNLVCSN---------YKTFSSLFPQTNLLSFHHQIHEGVDDICSSSKPNRCLQR 241
             NS+     +          Y+ F S    +N+ S +                   L+R
Sbjct: 184 KSNSTIEFAETTIYPPPPSHMYEQFPSTDSDSNITSKY------------------VLKR 243

Query: 242 FVFGAKAISDLKAKAKSTEVPNPTSVEALTGFIWNCAMKLKATSMGNDDDSQRPSTLSHV 301
           FVF    I++LK KA S  VP PT VEA+   IW CA     +++       R + +   
Sbjct: 244 FVFEPSKIAELKHKAASESVPVPTRVEAIMSLIWRCARNSSRSNL----LIPRQAVMWQA 303

Query: 302 VNLRKRMEPPL-PEISIGNVLWGMVAHYCSSNYETQVELRDLVVLLRQSFSKINNDFIKG 361
           +++R R+   + P+  IGN+  G        + E++ E+ ++V   R++  ++N    + 
Sbjct: 304 MDIRLRIPSSVAPKDVIGNLQSGF---SLKKDAESEFEIPEIVATFRKNKERVNEMIKES 363

Query: 362 LMGNDGNRAVLELLGEINKFPSKVPNYYFFTSWRNMDLNEVDFGWGKPIWIGIAGNSNVI 421
           L GN   +++L L+ E     +++ + Y  +SW      EVDFG G P+W+G A ++   
Sbjct: 364 LQGNTIGQSLLSLMAETVSESTEI-DRYIMSSWCRKPFYEVDFGSGSPVWVGYASHTIYD 423

Query: 422 TKDFILLLDTPSGDGIEAWMVLDQKKMKLLQQNPQFLEFASLNPSI 457
               ++L+D+  GDG+EAW+ L ++ M +   + + L +A LNP +
Sbjct: 424 NMVGVVLIDSKEGDGVEAWISLPEEDMSVFVDDQELLAYAVLNPPV 433

BLAST of Moc05g08520 vs. ExPASy Swiss-Prot
Match: I3PLR4 ((13S,14R)-1,13-dihydroxy-N-methylcanadine 13-O-acetyltransferase AT1 OS=Papaver somniferum OX=3469 GN=AT1 PE=1 SV=1)

HSP 1 Score: 196.1 bits (497), Expect = 9.1e-49
Identity = 143/465 (30.75%), Postives = 224/465 (48.17%), Query Frame = 0

Query: 3   VKILSRETIMPSSPTPPHLKTFELSLIDQLSPPLYIPLLFFYPIKNKHDLETHNQKKAIA 62
           V+++S+ETI P +PTP  L+ + +SL+DQ S  +Y+P++ FYP  +  +         + 
Sbjct: 9   VEVISKETIKPRNPTPYQLRNYNMSLLDQYSSLVYVPIILFYPAASDANSTGSKHHDDLH 68

Query: 63  TLKNSLSKTLTNFFLLAGRITNTSIRCSNDEGAVFIEATVNCNISDILKEPNNEVLTKLL 122
            LK SLS+TL +F+ +AGR+ +      NDEG  F E  +   + D + + ++  L+ LL
Sbjct: 69  LLKRSLSETLVHFYPMAGRMKDNMTVDCNDEGIDFFEVRIKGRMCDFMMK-SDAHLSLLL 128

Query: 123 PCSIQCTKPTEEYAQIVAQANIFNCGGIVISLCLLHKLMDGISFSCFLKSWAS----INK 182
           P  +  T   +E AQ++ Q N+F+CGG  I  C+ +K+ D  +   F++S A       +
Sbjct: 129 PSEVASTNFVKE-AQVIVQVNMFDCGGTAICFCISNKIADACTMITFIRSLAGTTNIARR 188

Query: 183 GS---LDNSSQNLVCSNYKTFSSLFPQTNLLSFHHQIHEGVDDICSSSKPNRCLQRFVFG 242
           GS      ++QNLV S   T  SLFP +  L+   Q+     D  S+S      +RFVF 
Sbjct: 189 GSSIAAPTTNQNLVPSFDST--SLFPPSEQLA--SQVSYPTQD--STSVDKLVSKRFVFD 248

Query: 243 AKAISDLKAKAKST-----EVPNPTSVEALTGFIWNCAMKLKATSMGNDDDSQRPSTLSH 302
           A  I+  + K +S      +   PT VE ++  IW  A+K               ST++H
Sbjct: 249 AAKITSAREKLQSLMHDKYKCHRPTRVEVVSALIWKSAVKSAPPG--------SISTVTH 308

Query: 303 VVNLRKRMEPPLPEISIGNVLWGMVA---------------HYCSSNYETQV---ELRDL 362
            +N RK+M+PPL + S GN+   + A                  S++ E QV   EL D 
Sbjct: 309 AMNFRKKMDPPLQDASFGNLCVVVTAVLPATTATTTNPATKKVSSTSNEEQVALDELSDF 368

Query: 363 VVLLRQSFSKINND-------FIKGLMGNDGNRAVLELLGEINKFPSKVPNYYFFTSWRN 422
           V LLR+   K+  D         K + G+D +      + + +    KV    F TSW  
Sbjct: 369 VALLRREIDKVKGDKGCMEKIIQKFIYGHDAS------VAKDSDVEDKV-TALFMTSWCK 428

Query: 423 MDLNEVDFGWGKPIWIGIAGNSNVITKDFILLLDTPSGDGIEAWM 431
               E DFGWG P+W+          K+ + + D   G+GIE W+
Sbjct: 429 FGFYEADFGWGTPVWVTTVPLIEPKYKNMVFMNDMKCGEGIEVWV 450

BLAST of Moc05g08520 vs. ExPASy Swiss-Prot
Match: A0A2K8FQU5 (Tabersonine-19-hydroxy-O-acetyltransferase OS=Catharanthus roseus OX=4058 GN=TAT PE=1 SV=1)

HSP 1 Score: 195.7 bits (496), Expect = 1.2e-48
Identity = 146/455 (32.09%), Postives = 220/455 (48.35%), Query Frame = 0

Query: 3   VKILSRETIMPSSPTPPHLKTFELSLIDQLSPPLYIPLLFFYPIKNKHDLETHNQKKAIA 62
           V+I+S+  I PSSPTP  L+ + LS IDQ        L FFY         +H   K   
Sbjct: 6   VEIVSKMFIKPSSPTPQSLRRYNLSSIDQTIDSEVTSLAFFYTYN-----PSHESSKIGD 65

Query: 63  TLKNSLSKTLTNFFLLAGRITNTSIRCSNDEGAVFIEATVNCNISDILKEPNNEVLTKLL 122
            LKNSLSKTL +++  AGR+        NDEG  F+E  ++  ++DILK   +     +L
Sbjct: 66  LLKNSLSKTLVSYYQFAGRLIENDYIDCNDEGVEFVEVRIHGRMNDILKRGKSFATDLVL 125

Query: 123 PCSIQCTKPTEEYAQIVAQANIFNCGGIVISLCLLHKLMDGISFSCFLKSWASINKGSLD 182
           P  I       E + ++ Q + F+CGGI I     HK+ DG+S   F+K WAS    S  
Sbjct: 126 PTRIIA---LHEDSLLIVQLSHFDCGGIAIGFGASHKVSDGVSNVMFMKDWASSTSLSTF 185

Query: 183 NSSQNLVCSNYKTFSSLF-PQTNLLSFHHQIHEGVDDICSSSKPNRCL-QRFVFGAKAIS 242
           +    L+     T  S+F P+ N L  +  I              +CL +RFVF  +AI 
Sbjct: 186 HKPTPLL-----TADSIFPPEDNKLLSNKSI----------VSFQQCLGKRFVFSTEAIE 245

Query: 243 DLKAKAKSTEVPNPTSVEALTGFIWNCAMKLKATSMGNDDDSQRPSTLSHVVNLRKRMEP 302
            LK+KA    +  P+ VE +T F+  CA          D   ++P  +   VNLR  +  
Sbjct: 246 KLKSKAIEYGIQKPSRVEVVTAFLCQCAANC-------DLPRKKPYAIISAVNLRPYL-- 305

Query: 303 PLPEISIGNVLWGMVAHYCSSNYETQVELRDLVVLLRQSFSKINNDFI--KGLMGNDGNR 362
            LP+ SIGN+      ++C ++     +   L+  LR    K+  + I  + L      +
Sbjct: 306 ALPQNSIGNI---FSFYFCINDEGMDNQFSALISKLRNGKQKLLENIISKEKLTYESQMQ 365

Query: 363 AVLELLGEINKFPSKVPNYYFFTSWRNMDLNEVDFGWGKPIWIGIAGNSNVITKDFILLL 422
            + + L ++N       + YF +SW    + ++DFGWGKPI   +        KD ILL+
Sbjct: 366 ELTKCLDQLN---ISSLDTYFCSSWCRFPVYDIDFGWGKPI---LVSPFQPHVKDLILLM 419

Query: 423 DTPSGDGIEAWMVLDQKKMKLLQQNPQFLEFASLN 454
           D+P GDGIEA + +++KKM   ++N +   FA L+
Sbjct: 426 DSPEGDGIEALITMEEKKMAAFEKNEELRSFAYLD 419

BLAST of Moc05g08520 vs. ExPASy Swiss-Prot
Match: A0A2P1GIW7 (Stemmadenine O-acetyltransferase OS=Catharanthus roseus OX=4058 GN=SAT PE=1 SV=1)

HSP 1 Score: 193.7 bits (491), Expect = 4.5e-48
Identity = 137/450 (30.44%), Postives = 226/450 (50.22%), Query Frame = 0

Query: 2   EVKILSRETIMPSSPTPPHLKTFELSLIDQLSPPLYIPLLFFYPIKNKHDLETHNQKKAI 61
           +++ILS E I PSSPTP  LKT +LS +DQ+    +IP++ FYP +   +L+   + +  
Sbjct: 4   QMQILSEELIQPSSPTPQTLKTHKLSHLDQVLLTCHIPIILFYPNQLDSNLDRAQRSE-- 63

Query: 62  ATLKNSLSKTLTNFFLLAGRIT-NTSIRCSNDEGAVFIEATVNCNISDILKEPNNEVLTK 121
             LK SLS  LT F+ LAGRI  N+S+ C ND G  F+EA V+  +S+ +K    + L +
Sbjct: 64  -NLKRSLSTVLTQFYPLAGRININSSVDC-NDSGVPFLEARVHSQLSEAIKNVAIDELNQ 123

Query: 122 LLPCSIQCTKPTEEYA-----QIVAQANIFNCGGIVISLCLLHKLMDGISFSCFLKSWAS 181
            LP   Q     EE        +  + + F CGG  I +C+ HK+ D +S + FL SW +
Sbjct: 124 YLP--FQPYPGGEESGLKKDIPLAVKISCFECGGTAIGVCISHKIADALSLATFLNSWTA 183

Query: 182 INKGSLDNSSQNLVCSNYKTFSSLFPQTNLLSFHHQIHEGVDDICSSSKPNRCLQRFVFG 241
             +   D     +V  N+   S  FP    +     + +           N  ++RFVF 
Sbjct: 184 TCQEETD-----IVQPNFDLGSHHFPPMESIPAPEFLPD----------ENIVMKRFVFD 243

Query: 242 AKAISDLKAK--AKSTEVPNPTSVEALTGFIWNCAMKLKATSMGNDDDSQRPSTLSHVVN 301
            + +  LKA+  + +TEV N + V+ +   IW   + +         D++     +  VN
Sbjct: 244 KEKLEALKAQLASSATEVKNSSRVQIVIAVIWKQFIDVTRAKF----DTKNKLVAAQAVN 303

Query: 302 LRKRMEPPLPEISIGNVLWGMVAHYCSSNYETQVELRDLVVLLRQSFSKINNDFIKGLMG 361
           LR RM PP P+ ++GN     +A    +  E   +  DLV  L+ S +KI+++ +K L  
Sbjct: 304 LRSRMNPPFPQSAMGN-----IATMAYAVAEEDKDFSDLVGPLKTSLAKIDDEHVKEL-- 363

Query: 362 NDGNRAVLELLGEINKFPSKVPNYYFFTSWRNMDLNEVDFGWGKPIWIGIAGNSNVITKD 421
               + V  L      + ++    + F+SW  +   ++DFGWGKP+ +     + V  K+
Sbjct: 364 ---QKGVTYL-----DYEAEPQELFSFSSWCRLGFYDLDFGWGKPVSV---CTTTVPMKN 410

Query: 422 FILLLDTPSGDGIEAWMVLDQKKMKLLQQN 444
            + L+DT + DG+EAW+ + + +M +L  +
Sbjct: 424 LVYLMDTRNEDGMEAWISMAEDEMSMLSSD 410

BLAST of Moc05g08520 vs. ExPASy TrEMBL
Match: A0A6J1CAD9 (BAHD acyltransferase BIA1-like OS=Momordica charantia OX=3673 GN=LOC111009425 PE=3 SV=1)

HSP 1 Score: 947.2 bits (2447), Expect = 2.6e-272
Identity = 467/467 (100.00%), Postives = 467/467 (100.00%), Query Frame = 0

Query: 1   MEVKILSRETIMPSSPTPPHLKTFELSLIDQLSPPLYIPLLFFYPIKNKHDLETHNQKKA 60
           MEVKILSRETIMPSSPTPPHLKTFELSLIDQLSPPLYIPLLFFYPIKNKHDLETHNQKKA
Sbjct: 1   MEVKILSRETIMPSSPTPPHLKTFELSLIDQLSPPLYIPLLFFYPIKNKHDLETHNQKKA 60

Query: 61  IATLKNSLSKTLTNFFLLAGRITNTSIRCSNDEGAVFIEATVNCNISDILKEPNNEVLTK 120
           IATLKNSLSKTLTNFFLLAGRITNTSIRCSNDEGAVFIEATVNCNISDILKEPNNEVLTK
Sbjct: 61  IATLKNSLSKTLTNFFLLAGRITNTSIRCSNDEGAVFIEATVNCNISDILKEPNNEVLTK 120

Query: 121 LLPCSIQCTKPTEEYAQIVAQANIFNCGGIVISLCLLHKLMDGISFSCFLKSWASINKGS 180
           LLPCSIQCTKPTEEYAQIVAQANIFNCGGIVISLCLLHKLMDGISFSCFLKSWASINKGS
Sbjct: 121 LLPCSIQCTKPTEEYAQIVAQANIFNCGGIVISLCLLHKLMDGISFSCFLKSWASINKGS 180

Query: 181 LDNSSQNLVCSNYKTFSSLFPQTNLLSFHHQIHEGVDDICSSSKPNRCLQRFVFGAKAIS 240
           LDNSSQNLVCSNYKTFSSLFPQTNLLSFHHQIHEGVDDICSSSKPNRCLQRFVFGAKAIS
Sbjct: 181 LDNSSQNLVCSNYKTFSSLFPQTNLLSFHHQIHEGVDDICSSSKPNRCLQRFVFGAKAIS 240

Query: 241 DLKAKAKSTEVPNPTSVEALTGFIWNCAMKLKATSMGNDDDSQRPSTLSHVVNLRKRMEP 300
           DLKAKAKSTEVPNPTSVEALTGFIWNCAMKLKATSMGNDDDSQRPSTLSHVVNLRKRMEP
Sbjct: 241 DLKAKAKSTEVPNPTSVEALTGFIWNCAMKLKATSMGNDDDSQRPSTLSHVVNLRKRMEP 300

Query: 301 PLPEISIGNVLWGMVAHYCSSNYETQVELRDLVVLLRQSFSKINNDFIKGLMGNDGNRAV 360
           PLPEISIGNVLWGMVAHYCSSNYETQVELRDLVVLLRQSFSKINNDFIKGLMGNDGNRAV
Sbjct: 301 PLPEISIGNVLWGMVAHYCSSNYETQVELRDLVVLLRQSFSKINNDFIKGLMGNDGNRAV 360

Query: 361 LELLGEINKFPSKVPNYYFFTSWRNMDLNEVDFGWGKPIWIGIAGNSNVITKDFILLLDT 420
           LELLGEINKFPSKVPNYYFFTSWRNMDLNEVDFGWGKPIWIGIAGNSNVITKDFILLLDT
Sbjct: 361 LELLGEINKFPSKVPNYYFFTSWRNMDLNEVDFGWGKPIWIGIAGNSNVITKDFILLLDT 420

Query: 421 PSGDGIEAWMVLDQKKMKLLQQNPQFLEFASLNPSISIKGEVEDLNL 468
           PSGDGIEAWMVLDQKKMKLLQQNPQFLEFASLNPSISIKGEVEDLNL
Sbjct: 421 PSGDGIEAWMVLDQKKMKLLQQNPQFLEFASLNPSISIKGEVEDLNL 467

BLAST of Moc05g08520 vs. ExPASy TrEMBL
Match: A0A0A0KXH2 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G016510 PE=3 SV=1)

HSP 1 Score: 555.1 bits (1429), Expect = 2.9e-154
Identity = 295/477 (61.84%), Postives = 365/477 (76.52%), Query Frame = 0

Query: 1   MEVKILSRETIMPSSPTPPHLKTFELSLIDQLSPPLYIPLLFFYPIKNKHD-LETHNQ-- 60
           MEVK+LS+ETI+PSSPTPPHL+TF+LSL+DQLSP LYIPLL FYP+K  +D ++ H+Q  
Sbjct: 1   MEVKVLSKETIIPSSPTPPHLQTFQLSLLDQLSPMLYIPLLLFYPMKRSYDHIDQHDQDP 60

Query: 61  KKAIATLKNSLSKTLTNFFLLAGRITNTSIRCSNDEGAVFIEATVNCNISDILKEPNNEV 120
           KK IATLK SLSKTL+ F+LLAGRI + SI C ND+GAVF+EAT+N N+ DILKEPNNEV
Sbjct: 61  KKTIATLKTSLSKTLSRFYLLAGRIIDKSIHC-NDKGAVFMEATINTNMFDILKEPNNEV 120

Query: 121 LTKLLPCSIQC-TKPTEEYAQIVAQANIFNCGGIVISLCLLHKLMDGISFSCFLKSWASI 180
           LTKLLPCS+ C TKP EEY QIV QAN+F CGGI ISLCLLHKL+D  +F CFL+SWA+ 
Sbjct: 121 LTKLLPCSLLCNTKPIEEYPQIVVQANVFKCGGIAISLCLLHKLIDAATFCCFLRSWATT 180

Query: 181 NK---GSLDNSS-QNLVCSNYKTFSSLFPQTNLLSFHHQIHEGVDDICSSS---KPNRCL 240
           N+     LD+SS  N+VC +YK+FSSLFPQTNLL FH ++    + +   S      R L
Sbjct: 181 NRELLSQLDHSSPNNMVCFDYKSFSSLFPQTNLLPFHQRLINNDNAVILPSSIFNGKRRL 240

Query: 241 QRFVFGAKAISDLKAKAKSTEVPNPTSVEALTGFIWNCAMKLKATSMGNDDDSQRPSTLS 300
           QRFVF +KAI DLKAKAKS ++PNPT VEA+T FIW   MK     + +  DSQ PSTLS
Sbjct: 241 QRFVFRSKAILDLKAKAKSCDIPNPTCVEAITCFIWKYLMK-----VADGGDSQMPSTLS 300

Query: 301 HVVNLRKRMEPPLPEISIGNVLWGMVAHYCSSNYETQ----VELRDLVVLLRQSFSKINN 360
           HVVN+RK +EP L E+S+GN++WG VAH+ S+    +    +EL  LV LLRQS  KIN 
Sbjct: 301 HVVNIRKMIEPSLGEVSLGNIMWGTVAHHFSTTRPNESFEGLELSKLVSLLRQSLKKINK 360

Query: 361 DFIKGL-MGNDGNR--AVLELLGEINKFPSKVPNYYFFTSWRNMDLNEVDFGWGKPIWIG 420
           D+IK L MG D  R   V++L+GEINK+P  + NYYFFTSW+NM LNE+DFGWGKP+W G
Sbjct: 361 DYIKELIMGGDKGRRNGVMKLVGEINKWP--ISNYYFFTSWKNMKLNELDFGWGKPLWFG 420

Query: 421 IAGNSNVITKDFILLLDTPSGDG-IEAWMVLDQKKMKLLQQNPQFLEFASLNPSISI 459
           IAG+SN +  + I+L+D  S DG IEAW++LD+K+M+LL+QNPQFLEFA LNPSI +
Sbjct: 421 IAGDSNEMMGNIIVLVDNVSDDGSIEAWILLDEKEMQLLEQNPQFLEFALLNPSIHL 469

BLAST of Moc05g08520 vs. ExPASy TrEMBL
Match: A0A5D3CZ08 (Salutaridinol 7-O-acetyltransferase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold130G001080 PE=3 SV=1)

HSP 1 Score: 551.6 bits (1420), Expect = 3.2e-153
Identity = 277/484 (57.23%), Postives = 355/484 (73.35%), Query Frame = 0

Query: 1   MEVKILSRETIMPSSPTPPHLKTFELSLIDQLSPPLYIPLLFFYPIKNKHDLETH-NQKK 60
           MEVK+LS+E +MPSSPTPPHLKTF+LSL+DQ SP LY PL+ FYP+ NK D   H + ++
Sbjct: 1   MEVKVLSKENVMPSSPTPPHLKTFQLSLLDQFSPVLYAPLIIFYPMDNKDDPRHHQSHEE 60

Query: 61  AIATLKNSLSKTLTNFFLLAGRITNTSIRCSNDEGAVFIEATVNCNISDILKEPNNEVLT 120
            +ATLK+SLSKTL++F+LLAGRI + SIRCS DEGAVFIEATV+C++S+ILK+PNNE L 
Sbjct: 61  LMATLKSSLSKTLSHFYLLAGRIVDKSIRCS-DEGAVFIEATVSCSMSEILKQPNNEFLM 120

Query: 121 KLLPCSIQCTKPTEEYAQIVAQANIFNCGGIVISLCLLHKLMDGISFSCFLKSWASINKG 180
           KL+PCS +CTKP EEYA ++ QAN+F+CGGI ISLCLLHKLMD  +  CFLK WA+IN+ 
Sbjct: 121 KLIPCSERCTKPIEEYAHVIVQANVFDCGGIAISLCLLHKLMDATTIGCFLKCWATINRR 180

Query: 181 S-------LDNSSQNL------------------VCSNYKTFSSLFPQTNLLSFHHQIHE 240
           S       +DN +  L                  VC +YK  SSLFPQTN L FH ++HE
Sbjct: 181 SFTTTNLTIDNGASELLTSSSMTKDDNSDPFSKMVCYDYKELSSLFPQTNFLPFHPRLHE 240

Query: 241 GVDDICSSSKPNRCLQRFVFGAKAISDLKAKAKSTEVPNPTSVEALTGFIWNCAMKLKAT 300
            +   C         QRFVF  KAI DLKAKAKS +VPNPTSVE L+GFIW CA++  +T
Sbjct: 241 ALSSNCEEKS---SFQRFVFKEKAILDLKAKAKSNDVPNPTSVEVLSGFIWKCALEAAST 300

Query: 301 SMGNDDDSQRPSTLSHVVNLRKRMEPPLPEISIGNVLWGMVAHYCSSNYETQVELRDLVV 360
            +G    SQ PS L+H VNLRKRMEPPLPE S+GN+ W +VAHY +   +TQVEL +LV 
Sbjct: 301 KLGL---SQIPSILTHAVNLRKRMEPPLPEFSVGNIFWNVVAHYLADK-KTQVELSELVS 360

Query: 361 LLRQSFSKINNDFIKGLMGNDGNRAVLELLGEINKFPSKVPNYYFFTSWRNMDLNEVDFG 420
           L+RQSF  IN ++IK ++GN+G+ A+++L+ E N    ++P  Y  TSWRNMDL+EV+FG
Sbjct: 361 LIRQSFVDINGNYIKKIVGNEGSEALVKLVWERNMKLFQIPKLYICTSWRNMDLSEVNFG 420

Query: 421 WGKPIWIGIAGNSNVITKDFILLLDTPSGDGIEAWMVLDQKKMKLLQQNPQFLEFASLNP 459
           WGKPIWIG AGNSN   K+ I+LLD  S DG+EAW++L++++M++L +N +FL FA LNP
Sbjct: 421 WGKPIWIGSAGNSNTTIKNMIVLLDAISNDGVEAWVILEEEEMQILLENQEFLNFALLNP 476

BLAST of Moc05g08520 vs. ExPASy TrEMBL
Match: A0A1S3BY96 (salutaridinol 7-O-acetyltransferase-like OS=Cucumis melo OX=3656 GN=LOC103494518 PE=3 SV=1)

HSP 1 Score: 551.6 bits (1420), Expect = 3.2e-153
Identity = 277/484 (57.23%), Postives = 355/484 (73.35%), Query Frame = 0

Query: 1   MEVKILSRETIMPSSPTPPHLKTFELSLIDQLSPPLYIPLLFFYPIKNKHDLETH-NQKK 60
           MEVK+LS+E +MPSSPTPPHLKTF+LSL+DQ SP LY PL+ FYP+ NK D   H + ++
Sbjct: 1   MEVKVLSKENVMPSSPTPPHLKTFQLSLLDQFSPVLYAPLIIFYPMDNKDDPRHHQSHEE 60

Query: 61  AIATLKNSLSKTLTNFFLLAGRITNTSIRCSNDEGAVFIEATVNCNISDILKEPNNEVLT 120
            +ATLK+SLSKTL++F+LLAGRI + SIRCS DEGAVFIEATV+C++S+ILK+PNNE L 
Sbjct: 61  LMATLKSSLSKTLSHFYLLAGRIVDKSIRCS-DEGAVFIEATVSCSMSEILKQPNNEFLM 120

Query: 121 KLLPCSIQCTKPTEEYAQIVAQANIFNCGGIVISLCLLHKLMDGISFSCFLKSWASINKG 180
           KL+PCS +CTKP EEYA ++ QAN+F+CGGI ISLCLLHKLMD  +  CFLK WA+IN+ 
Sbjct: 121 KLIPCSERCTKPIEEYAHVIVQANVFDCGGIAISLCLLHKLMDATTIGCFLKCWATINRR 180

Query: 181 S-------LDNSSQNL------------------VCSNYKTFSSLFPQTNLLSFHHQIHE 240
           S       +DN +  L                  VC +YK  SSLFPQTN L FH ++HE
Sbjct: 181 SFTTTNLTIDNGASELLTSSSMTKDDNSDPFSKMVCYDYKELSSLFPQTNFLPFHPRLHE 240

Query: 241 GVDDICSSSKPNRCLQRFVFGAKAISDLKAKAKSTEVPNPTSVEALTGFIWNCAMKLKAT 300
            +   C         QRFVF  KAI DLKAKAKS +VPNPTSVE L+GFIW CA++  +T
Sbjct: 241 ALSSNCEEKS---SFQRFVFKEKAILDLKAKAKSNDVPNPTSVEVLSGFIWKCALEAAST 300

Query: 301 SMGNDDDSQRPSTLSHVVNLRKRMEPPLPEISIGNVLWGMVAHYCSSNYETQVELRDLVV 360
            +G    SQ PS L+H VNLRKRMEPPLPE S+GN+ W +VAHY +   +TQVEL +LV 
Sbjct: 301 KLGL---SQIPSILTHAVNLRKRMEPPLPEFSVGNIFWNVVAHYLADK-KTQVELSELVS 360

Query: 361 LLRQSFSKINNDFIKGLMGNDGNRAVLELLGEINKFPSKVPNYYFFTSWRNMDLNEVDFG 420
           L+RQSF  IN ++IK ++GN+G+ A+++L+ E N    ++P  Y  TSWRNMDL+EV+FG
Sbjct: 361 LIRQSFVDINGNYIKKIVGNEGSEALVKLVWERNMKLFQIPKLYICTSWRNMDLSEVNFG 420

Query: 421 WGKPIWIGIAGNSNVITKDFILLLDTPSGDGIEAWMVLDQKKMKLLQQNPQFLEFASLNP 459
           WGKPIWIG AGNSN   K+ I+LLD  S DG+EAW++L++++M++L +N +FL FA LNP
Sbjct: 421 WGKPIWIGSAGNSNTTIKNMIVLLDAISNDGVEAWVILEEEEMQILLENQEFLNFALLNP 476

BLAST of Moc05g08520 vs. ExPASy TrEMBL
Match: A0A6J1GVT5 (salutaridinol 7-O-acetyltransferase-like OS=Cucurbita moschata OX=3662 GN=LOC111458004 PE=3 SV=1)

HSP 1 Score: 545.4 bits (1404), Expect = 2.3e-151
Identity = 286/464 (61.64%), Postives = 355/464 (76.51%), Query Frame = 0

Query: 1   MEVKILSRETIMPSSPTPPHLKTFELSLIDQLSPPLYIPLLFFYPIKNKHDLETHNQKKA 60
           MEVK+LSRETI+PSSPTPPHLKTFELSL+DQLSP LY PLLFFYPI      +T++    
Sbjct: 1   MEVKVLSRETIIPSSPTPPHLKTFELSLLDQLSPMLYTPLLFFYPI-----TKTYDPNPT 60

Query: 61  IATLKNSLSKTLTNFFLLAGRITNTSIRCSNDEGAVFIEATVNCNISDILKEPNNEVLTK 120
           +ATLK+SLS+TLT F+LLAGRI   SI CS D+GAVFIEA+V+CN+S ILK+PNNEVL K
Sbjct: 61  MATLKDSLSQTLTRFYLLAGRIVGKSIHCS-DQGAVFIEASVSCNMSHILKQPNNEVLRK 120

Query: 121 LLPCSIQCTKPTEEYAQIVAQANIFNCGGIVISLCLLHKLMDGISFSCFLKSWASINKGS 180
           LLPCS+ C+ P EE  Q+V QANIF+CGGI ISLCLLHKLMDG +F+ FL SWAS NKGS
Sbjct: 121 LLPCSLLCSNPAEESPQVVVQANIFSCGGIAISLCLLHKLMDGATFTSFLTSWASTNKGS 180

Query: 181 LDNSSQNLVCSNYKTFSSLFPQTNLLSFHHQIHEGVDDICSSSKPNRCLQRFVFGAKAIS 240
           LD+SS N++C +YK FSSLFPQTNLL FH +       +   +   RCL+RFVFG+K++ 
Sbjct: 181 LDSSS-NMICYDYKAFSSLFPQTNLLPFHQK-------LAKPANRKRCLRRFVFGSKSLL 240

Query: 241 DLKAKAKSTEVPNPTSVEALTGFIWNCAMKLKATSMGNDDDSQRPSTLSHVVNLRKRMEP 300
           +LKAKA+S  VPNP+ VEALTGFIW  AM           D Q  STLSHVVNLR RM+P
Sbjct: 241 NLKAKAESRAVPNPSCVEALTGFIWKHAMMAA--------DCQGSSTLSHVVNLRGRMKP 300

Query: 301 PLPEISIGNVLWGMVAHYCSSNYETQVELRDLVVLLRQSFSKINNDFI-KGLMGND-GNR 360
            LPE+S+GN++WG VAH   S+   ++ELR LV LLRQSF +IN D+I + L+G+D G  
Sbjct: 301 ALPEVSVGNIMWGAVAH---SSAMERLELRALVGLLRQSFEEINKDYIERMLIGSDKGFE 360

Query: 361 AVLELLGEINKFPSKVPNYYFFTSWRNMDLNEVDFGWGKPIWIGIAGNSNVITKDFILLL 420
           AVL+ +GE+NK+   + NYYF TSWRNM LNEVDFGWG+P WI IA ++N +  + I+L+
Sbjct: 361 AVLKFVGEMNKW--GMSNYYFCTSWRNMKLNEVDFGWGRPAWIAIAWDTNELMANVIVLV 420

Query: 421 DTPSGDGIEAWMVLDQKKMKLLQQNPQFLEFASLNPSISIKGEV 463
           D   GDGIEAW++LD+K+M++L+QNPQFLEFA LNPSI +   +
Sbjct: 421 DGVMGDGIEAWILLDEKEMEILEQNPQFLEFALLNPSIYLADNI 437

BLAST of Moc05g08520 vs. TAIR 10
Match: AT3G26040.1 (HXXXD-type acyl-transferase family protein )

HSP 1 Score: 270.4 bits (690), Expect = 2.7e-72
Identity = 173/464 (37.28%), Postives = 265/464 (57.11%), Query Frame = 0

Query: 1   MEVKILSRETIMPSSPTPPHLKTFELSLIDQLSPPLYIPLLFFYPIKNKHDLETHNQKKA 60
           M V ++SR+ I PSSPTP HLK F+LSL++QL P ++ P++FFY   N     +    + 
Sbjct: 1   MRVDVVSRDIIKPSSPTPNHLKKFKLSLLEQLGPTIFGPMVFFYSANN-----SIKPTEQ 60

Query: 61  IATLKNSLSKTLTNFFLLAGRIT-NTSIRCSNDEGAVFIEATVNCNISDILKEPNNEVLT 120
           +  LK SLS+TLT+F+ LAGR+  N SI C ND GA F+EA VN  +S++L EP+++ L 
Sbjct: 61  LQMLKKSLSETLTHFYPLAGRLKGNISIDC-NDSGADFLEARVNSPLSNLLLEPSSDSLQ 120

Query: 121 KLLPCSIQCTKPTEEYAQIVAQANIFNCGGIVISLCLLHKLMDGISFSCFLKSWASINKG 180
           +L+P S+   +       ++AQA+ F CG + I +C+ HKL D  S   F+KSWA+I   
Sbjct: 121 QLIPTSVDSIETRTRL--LLAQASFFECGSMSIGVCISHKLADATSIGLFMKSWAAI--- 180

Query: 181 SLDNSSQNLVCSNYKTFSSLFPQTNLLSFHHQIHEGVDDICSSSKPNRCL-QRFVFGAKA 240
           S   S + +    + T   +FP  N              +      N+ L +RF+F + +
Sbjct: 181 SSRGSIKTIGAPVFDTV-KIFPPGNFSE-----TSPAPVVEPEIMMNQTLSKRFIFDSSS 240

Query: 241 ISDLKAKAKSTEVPNPTSVEALTGFIWNCAMKLKATSMGNDDDSQRPSTLSHVVNLRKRM 300
           I  L+AKA S EV  PT VEA++  IW  AMK   T  G    + +PS L++ V+LR R+
Sbjct: 241 IQALQAKASSFEVNQPTRVEAVSALIWKSAMKATRTVSG----TSKPSILANSVSLRSRV 300

Query: 301 EPPLPEISIGNVLWGMVAHYCSSNYE--TQVELRDLVVLLRQSFSKINNDFIKGLMGNDG 360
            PP  + SIGN    +V+++ +   E   Q +L+ LV  +R++  +  +  I  L+GN  
Sbjct: 301 SPPFTKNSIGN----LVSYFAAKAEEGINQTKLQTLVSKIRKAKQRFRDIHIPKLVGNPN 360

Query: 361 NRAVL----ELLGEINKFPSKVPNYYFFTSWRNMDLNEVDFGWGKPIWIGIAGNSNVITK 420
              ++    +  G++    S   ++Y F+S     L E DFGWGKP+W+G     +V  K
Sbjct: 361 ATEIICSYQKEAGDM--IASGDFDFYIFSSACRFGLYETDFGWGKPVWVGF---PSVRQK 420

Query: 421 DFILLLDTPSGDGIEAWMVLDQKKMKLLQQNPQFLEFASLNPSI 457
           + + LLDT    GIEAW+ L++++M L +Q+ + L+FASLNPS+
Sbjct: 421 NIVTLLDTKEAGGIEAWVNLNEQEMNLFEQDRELLQFASLNPSV 434

BLAST of Moc05g08520 vs. TAIR 10
Match: AT1G24420.1 (HXXXD-type acyl-transferase family protein )

HSP 1 Score: 246.1 bits (627), Expect = 5.5e-65
Identity = 167/460 (36.30%), Postives = 249/460 (54.13%), Query Frame = 0

Query: 3   VKILSRETIMPSSPTPPHLKTFELSLIDQLSPPLYIPLLFFYPIKNKHDLETHNQKKAIA 62
           V+ILSRE + PSSPTP   +   LSL+D LS P+Y   L FY    ++ L    ++ ++ 
Sbjct: 5   VEILSREIVKPSSPTPDDKRILNLSLLDILSSPMYTGALLFYAADPQNLLGFSTEETSL- 64

Query: 63  TLKNSLSKTLTNFFLLAGRITNTSIRCSNDEGAVFIEATVNCNISDILKEPNNEVLTKLL 122
            LK SLSKTL  F+ LAGRI  + + C NDEGAVFIEA V+  +S+ LK P  E L  L+
Sbjct: 65  KLKKSLSKTLPIFYPLAGRIIGSFVEC-NDEGAVFIEARVDHLLSEFLKCPVPESLELLI 124

Query: 123 PCSIQCTKPTEEYAQIVAQANIFNCGGIVISLCLLHKLMDGISFSCFLKSWASINKGSLD 182
           P   + ++    +  ++ QAN F+CGG+VI++C+ HK+ D  S + F++ WA  ++G   
Sbjct: 125 PVEAK-SREAVTWPVLLIQANFFSCGGLVITICVSHKITDATSLAMFIRGWAESSRG--- 184

Query: 183 NSSQNLVCSNYKTFSSLFPQ--TNLLSFHHQIHEGVDDICSSSKPNRCLQRFVFGAKAIS 242
                +      T S +FP+    L S      E V+++   +K      RFVF A  I 
Sbjct: 185 ---LGITLIPSFTASEVFPKPLDELPSKPMDRKEEVEEMSCVTK------RFVFDASKIK 244

Query: 243 DLKAKAKSTEVPNPTSVEALTGFIWNCAMKLKATSMGNDDDSQRPSTLSHVVNLRKRMEP 302
            L+AKA    V NPT VEA+T   W C  K+   S      + R S L  +VNLR +++ 
Sbjct: 245 KLRAKASRNLVKNPTRVEAVTALFWRCVTKVSRLS----SLTPRTSVLQILVNLRGKVD- 304

Query: 303 PLPEISIGNVLWGMVAHYCSSNYETQVELRDLVVLLRQSFSKINNDFIKGLMGNDGNRAV 362
            L E +IGN+L  M+     +  E   ++ D +   ++ FS    +  K       +  +
Sbjct: 305 SLCENTIGNMLSLMILKNEEAAIERIQDVVDEIRRAKEIFSLNCKEMSK------SSSRI 364

Query: 363 LELLGEINKFPSK--VPNYYFFTSWRNMDLNEVDFGWGKPIWIGIAGNSNVITKDFILLL 422
            ELL EI K   +    + +   SW  + L + DFGWGKP+W+   G S+   K+ +LL+
Sbjct: 365 FELLEEIGKVYGRGNEMDLWMSNSWCKLGLYDADFGWGKPVWVTGRGTSHF--KNLMLLI 424

Query: 423 DTPSGDGIEAWMVLDQKKMKLLQQNPQFLEFASLNPSISI 459
           DT  G+GIEAW+ L +++M L + + + LE ASLNP + I
Sbjct: 425 DTKDGEGIEAWITLTEEQMSLFECDQELLESASLNPPVLI 436

BLAST of Moc05g08520 vs. TAIR 10
Match: AT4G15390.1 (HXXXD-type acyl-transferase family protein )

HSP 1 Score: 238.0 bits (606), Expect = 1.5e-62
Identity = 154/472 (32.63%), Postives = 249/472 (52.75%), Query Frame = 0

Query: 1   MEVKILSRETIMPSSPTPPHLKTFELSLIDQLSPPLYIPLLFFYPIKNKHDLETHNQKKA 60
           M+V+ +S+E I PSSPTP +L+T +LS+ D + PP+Y     FY    K+DL   +Q+  
Sbjct: 6   MKVETISKEIIKPSSPTPNNLQTLQLSIYDHILPPVYTVAFLFY---TKNDL--ISQEHT 65

Query: 61  IATLKNSLSKTLTNFFLLAGRITNTSIRCSNDEGAVFIEATV-NCNISDILKEPNNEVLT 120
              LK SLS+TLT F+ LAGRIT  ++ C+ DEGA+F++A V NC +++ LK P+ + L 
Sbjct: 66  SHKLKTSLSETLTKFYPLAGRITGVTVDCT-DEGAIFVDARVNNCPLTEFLKCPDFDALQ 125

Query: 121 KLLPCSIQCTK--PTEEYAQIVAQANIFNCGGIVISLCLLHKLMDGISFSCFLKSWASIN 180
           +LLP  +          +  ++ +A  F CGG+ I +C+ HK+ D  S S F++SWA+  
Sbjct: 126 QLLPLDVVDNPYVAAATWPLLLVKATYFGCGGMAIGICITHKIADAASISTFIRSWAATA 185

Query: 181 KGSLDNSSQNLVCSNYKTFSSLFPQTNLLSFHHQIHEGVDDICSSSKPNRCLQRFVFGAK 240
           +G  D ++     S     ++ +P  N  +F     E       + K +   +RFVF A 
Sbjct: 186 RGENDAAAME---SPVFAGANFYPPAN-EAFKLPADE------QAGKRSSITKRFVFEAS 245

Query: 241 AISDLKAKAKSTE-VPNPTSVEALTGFIWNCAMKLKATSMGNDDDSQRPSTLSHVVNLRK 300
            + DL+ KA S E V  PT VE++T  IW C +    T+  +         L  + NLR 
Sbjct: 246 KVEDLRTKAASEETVDQPTRVESVTALIWKCFVASSKTTTCDH------KVLVQLANLRS 305

Query: 301 RMEPPLPEISIGNVLWGMVAHYCSSNYETQVE--LRDL--------VVLLRQSFSKINND 360
           ++   L E SIGN+++  V        E ++E  +RDL         V+L +  S  ++ 
Sbjct: 306 KIPSLLQESSIGNLMFSSVVLSIGRGGEVKIEEAVRDLRKKKEELGTVILDEGGSSDSSS 365

Query: 361 FIKGLMGNDGNRAVLELLGEINKFPSKVPNYYFFTSWRNMDLNEVDFGWGKPIWIGIAGN 420
            I       G++    +L   ++   +    Y  +SW  + L E  FGW  P+W  + GN
Sbjct: 366 MI-------GSKLANLMLTNYSRLSYETHEPYTVSSWCKLPLYEASFGWDSPVW--VVGN 425

Query: 421 SNVITKDFILLLDTPSGDGIEAWMVLDQKKMKLLQQNPQFLEFASLNPSISI 459
            + +  +  +L+D+  G GIEA++ L ++ M   +QNP+ L FA++NPS+ +
Sbjct: 426 VSPVLGNLAMLIDSKDGQGIEAFVTLPEENMSSFEQNPELLAFATMNPSVLV 446

BLAST of Moc05g08520 vs. TAIR 10
Match: AT5G47950.1 (HXXXD-type acyl-transferase family protein )

HSP 1 Score: 234.6 bits (597), Expect = 1.6e-61
Identity = 157/464 (33.84%), Postives = 239/464 (51.51%), Query Frame = 0

Query: 1   MEVKILSRETIMPSSPTPPHLKTFELSLIDQLSPPLYIPLLFFYPIKNKHDLETHNQKKA 60
           M+V+ + +E I PS+ TP  L T +LS++D L PP+Y     FY    K DL   +Q++ 
Sbjct: 4   MKVETIGKEIIKPSATTPTDLSTLQLSIMDILMPPVYAVAFLFY---TKDDL--ISQEQT 63

Query: 61  IATLKNSLSKTLTNFFLLAGRITNTSIRCSNDEGAVFIEATV-NCNISDILKEPNNEVLT 120
             TLK SLS+ LT F  LAGR+   +I+ S DEGAVF+EA V NC++S  L+ P+ E L 
Sbjct: 64  SHTLKTSLSEILTKFHPLAGRVNGVTIK-STDEGAVFVEARVDNCDLSGFLRSPDTESLK 123

Query: 121 KLLPCSIQCTKPTEEYAQIVAQANIFNCGGIVISLCLLHKLMDGISFSCFLKSWASINKG 180
           +LLP      +P   +  ++ +A  F CGG+ I LC+ H+L D  S S FL++WA+  +G
Sbjct: 124 QLLPVD---DEPAPTWPLLLVKATYFRCGGMAIGLCISHRLADAASLSIFLQAWAATARG 183

Query: 181 SLDNSSQNLVCSNYKTFSSLFPQTNLLSFHHQIHEGVDDICSSSKPNRCLQRFVFGAKAI 240
             D  +    CS     + L+P  N         E +             +RFVF A  I
Sbjct: 184 ESDLVASPDFCS-----TKLYPAAN---------EAIKIPGEVVNRTSVTKRFVFVASKI 243

Query: 241 SDLKAKAKSTEVPNPTSVEALTGFIWNCAMKLKATSMGNDDDSQRPSTLSHVVNLRKRME 300
            +L+ K  S  VP PT V+++T  IW C +           D+ R   L    NLR ++ 
Sbjct: 244 EELRNKVASDVVPRPTRVQSVTSLIWKCVVTAST-------DTIREKALFQPANLRTKIP 303

Query: 301 PPLPEISIGNVLWGMVAHYCSSNYETQVELRDLVVLLRQSFSKINNDFIKGLMGNDGNRA 360
             L E  IGN+L+  +     +  +    +++L         K   D    +   +G+  
Sbjct: 304 SLLSENQIGNLLFNSLILDGKAGMDIVETVKEL--------QKRAEDLSGLVQDEEGSSM 363

Query: 361 VL--ELLGEI--NKFPSKVPNYYFFTSWRNMDLNEVDFGWGKPIWIGIAGNSNVITKDFI 420
            +   L GEI  +KF  +  + +  TSW  + L +  FGWG P+W+  + + N+   D++
Sbjct: 364 TIGWRLFGEIINSKFNREDHDMHSVTSWCKIPLYDACFGWGSPVWVAGSVSPNL---DYV 423

Query: 421 -LLLDTPSGDGIEAWMVLDQKKMKLLQQNPQFLEFASLNPSISI 459
            +L+D+  G GIEAW+ L Q+ M LL+Q+ + L FAS NPS+ I
Sbjct: 424 TVLIDSKDGQGIEAWVTLPQENMLLLEQSTELLAFASPNPSVLI 426

BLAST of Moc05g08520 vs. TAIR 10
Match: AT3G30280.1 (HXXXD-type acyl-transferase family protein )

HSP 1 Score: 229.6 bits (584), Expect = 5.3e-60
Identity = 160/468 (34.19%), Postives = 248/468 (52.99%), Query Frame = 0

Query: 1   MEVKILSRETIMPSSPTPPHLKTFELSLIDQLSPPLYIPLLFFYPIKNKHDLETHNQKKA 60
           M+V+ +++E I PSS TP  L+T +LS+ D + PP+Y     FY    K DL +  Q   
Sbjct: 4   MKVETIAKEIIKPSSTTPNDLQTLQLSIYDHILPPVYTVAFLFY---TKDDLISPEQSS- 63

Query: 61  IATLKNSLSKTLTNFFLLAGRITNTSIRCSNDEGAVFIEATV-NCNISDILKEPNNEVLT 120
              LK SL++TLT F+ LAGRI   +I C NDEGAVF++A V N  +SD L+ P+ + L 
Sbjct: 64  -HKLKTSLAETLTKFYPLAGRIKGVTIDC-NDEGAVFVDARVNNYPLSDFLRSPDFKTLQ 123

Query: 121 KLLPCSI--QCTKPTEEYAQIVAQANIFNCGGIVISLCLLHKLMDGISFSCFLKSWASIN 180
           +LLP  +     +    +  ++ +A  F CGG+ I LC+ HK+ D  S S F++SWA++ 
Sbjct: 124 QLLPLDVIDDPYEAATTWPLLLVKATYFPCGGMAIGLCITHKIADATSISTFIQSWAAMA 183

Query: 181 KGSLDNSSQNLVCSNYKTFSSLFPQTNLLSFHHQIHEGVDDICSSSKPNRCLQRFVFGAK 240
           +G     + ++V       ++ +P  N L F   + E  + I   +K      RFVF A 
Sbjct: 184 RG----EAGDIVADPVFAAANFYPPANEL-FKFPVDENANKISQITK------RFVFSAS 243

Query: 241 AISDLKAKAKSTE-VPNPTSVEALTGFIWNCAMKLKATSMGNDDDSQRPSTLSHVVNLRK 300
            + +L+ KA S + V  P  VE++T  +W  A    A+S  N  D +    L    NLR 
Sbjct: 244 KLEELRTKAASEDFVGRPKRVESVTALLWK-AFVAAASSNTNACDMK---VLIQPSNLRP 303

Query: 301 RMEPPLPEISIGNVLWGMVAHYCSSNYETQVE--LRDLVVLLRQSFSKINNDFIKGLMGN 360
           ++   L E  IGN+++  V    S   E ++E  +RD    LR+    + N  IK   G+
Sbjct: 304 KIPSLLAESLIGNIMFSSVVLGISQQEEIKIEKAVRD----LRKQGDDLQN-VIKEEGGS 363

Query: 361 D----GNRAVLELLGEINKFPSKVPNYYFFTSWRNMDLNEVDFGWGKPIWIGIAGNSNVI 420
                G++    +L   +K   +    Y  +SW  + L E  FGWG P+W  IAGN   +
Sbjct: 364 SSAMIGSKLANLMLTNYSKLSYETHEPYTVSSWCKLPLYEASFGWGSPVW--IAGNVAPM 423

Query: 421 TKDFILLLDTPSGDGIEAWMVLDQKKMKLLQQNPQFLEFASLNPSISI 459
            ++  +L+D+  G GIEA++ L ++ M  L+QN + L FAS+NPS+ +
Sbjct: 424 LENVTMLIDSKDGQGIEAFVTLPEENMLSLEQNTELLAFASVNPSVLV 443

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022138192.15.3e-272100.00BAHD acyltransferase BIA1-like [Momordica charantia][more]
XP_038875765.13.5e-15462.85BAHD acyltransferase BIA1-like [Benincasa hispida][more]
XP_004152093.15.9e-15461.84stemmadenine O-acetyltransferase [Cucumis sativus][more]
KAE8649121.11.7e-15361.38hypothetical protein Csa_015082 [Cucumis sativus][more]
XP_008453950.16.5e-15357.23PREDICTED: salutaridinol 7-O-acetyltransferase-like [Cucumis melo] >TYK16967.1 s... [more]
Match NameE-valueIdentityDescription
Q94FT41.7e-5031.40Salutaridinol 7-O-acetyltransferase OS=Papaver somniferum OX=3469 GN=SALAT PE=1 ... [more]
O233934.8e-5029.18BAHD acyltransferase BIA1 OS=Arabidopsis thaliana OX=3702 GN=BIA1 PE=2 SV=1[more]
I3PLR49.1e-4930.75(13S,14R)-1,13-dihydroxy-N-methylcanadine 13-O-acetyltransferase AT1 OS=Papaver ... [more]
A0A2K8FQU51.2e-4832.09Tabersonine-19-hydroxy-O-acetyltransferase OS=Catharanthus roseus OX=4058 GN=TAT... [more]
A0A2P1GIW74.5e-4830.44Stemmadenine O-acetyltransferase OS=Catharanthus roseus OX=4058 GN=SAT PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1CAD92.6e-272100.00BAHD acyltransferase BIA1-like OS=Momordica charantia OX=3673 GN=LOC111009425 PE... [more]
A0A0A0KXH22.9e-15461.84Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G016510 PE=3 SV=1[more]
A0A5D3CZ083.2e-15357.23Salutaridinol 7-O-acetyltransferase-like OS=Cucumis melo var. makuwa OX=1194695 ... [more]
A0A1S3BY963.2e-15357.23salutaridinol 7-O-acetyltransferase-like OS=Cucumis melo OX=3656 GN=LOC103494518... [more]
A0A6J1GVT52.3e-15161.64salutaridinol 7-O-acetyltransferase-like OS=Cucurbita moschata OX=3662 GN=LOC111... [more]
Match NameE-valueIdentityDescription
AT3G26040.12.7e-7237.28HXXXD-type acyl-transferase family protein [more]
AT1G24420.15.5e-6536.30HXXXD-type acyl-transferase family protein [more]
AT4G15390.11.5e-6232.63HXXXD-type acyl-transferase family protein [more]
AT5G47950.11.6e-6133.84HXXXD-type acyl-transferase family protein [more]
AT3G30280.15.3e-6034.19HXXXD-type acyl-transferase family protein [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (OHB3-1) v2
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR003480TransferasePFAMPF02458Transferasecoord: 1..446
e-value: 2.9E-81
score: 273.4
IPR023213Chloramphenicol acetyltransferase-like domain superfamilyGENE3D3.30.559.10coord: 228..448
e-value: 1.7E-55
score: 189.4
IPR023213Chloramphenicol acetyltransferase-like domain superfamilyGENE3D3.30.559.10coord: 1..213
e-value: 1.0E-55
score: 190.4
NoneNo IPR availablePANTHERPTHR31623:SF59HXXXD-TYPE ACYL-TRANSFERASE FAMILY PROTEINcoord: 4..456
NoneNo IPR availablePANTHERPTHR31623F21J9.9coord: 4..456

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Moc05g08520.1Moc05g08520.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0016747 acyltransferase activity, transferring groups other than amino-acyl groups