Moc05g08480 (gene) Bitter gourd (OHB3-1) v2

BLAST of Moc05g08480 vs. NCBI nr
Match: XP_022138133.1 (uncharacterized protein LOC111009380 isoform X1 [Momordica charantia])

HSP 1 Score: 741.9 bits (1914), Expect = 2.8e-210
Identity = 382/382 (100.00%), Postives = 382/382 (100.00%), Query Frame = 0

Query: 1   MGEAPAKALRRILDSPGVHLGPACFDALSAKLIERAGFSYCFTSGFSISASRLALPDTGF 60
           MGEAPAKALRRILDSPGVHLGPACFDALSAKLIERAGFSYCFTSGFSISASRLALPDTGF
Sbjct: 1   MGEAPAKALRRILDSPGVHLGPACFDALSAKLIERAGFSYCFTSGFSISASRLALPDTGF 60

Query: 61  MSYGEMLDQGQQITEAVSIPVIGDGDNGYGNALNVKRTIKGFIRAGFAGILLEDQVSPKA 120
           MSYGEMLDQGQQITEAVSIPVIGDGDNGYGNALNVKRTIKGFIRAGFAGILLEDQVSPKA
Sbjct: 61  MSYGEMLDQGQQITEAVSIPVIGDGDNGYGNALNVKRTIKGFIRAGFAGILLEDQVSPKA 120

Query: 121 CGHTRGRKVVPREEAVSCIRAAVDARKESGSDIVIVARTDARQAVSLDEALWRTKAFADA 180
           CGHTRGRKVVPREEAVSCIRAAVDARKESGSDIVIVARTDARQAVSLDEALWRTKAFADA
Sbjct: 121 CGHTRGRKVVPREEAVSCIRAAVDARKESGSDIVIVARTDARQAVSLDEALWRTKAFADA 180

Query: 181 GADVLFIDALASIEEMKAFCKISPEVPKMANMLEGGGKTPILNPLELAEIGYKVVVYPLS 240
           GADVLFIDALASIEEMKAFCKISPEVPKMANMLEGGGKTPILNPLELAEIGYKVVVYPLS
Sbjct: 181 GADVLFIDALASIEEMKAFCKISPEVPKMANMLEGGGKTPILNPLELAEIGYKVVVYPLS 240

Query: 241 LVGISIRAMQDALLALKEGQIPSERMPSFEEMKEILGFNSYYEEEEKYASAVSLPSQGDG 300
           LVGISIRAMQDALLALKEGQIPSERMPSFEEMKEILGFNSYYEEEEKYASAVSLPSQGDG
Sbjct: 241 LVGISIRAMQDALLALKEGQIPSERMPSFEEMKEILGFNSYYEEEEKYASAVSLPSQGDG 300

Query: 301 SKHPSSGILSRRLMVKITGKDGLEKLDVRIPAGSLDGVANIVPALGGVNIKELLNDAAEE 360
           SKHPSSGILSRRLMVKITGKDGLEKLDVRIPAGSLDGVANIVPALGGVNIKELLNDAAEE
Sbjct: 301 SKHPSSGILSRRLMVKITGKDGLEKLDVRIPAGSLDGVANIVPALGGVNIKELLNDAAEE 360

Query: 361 VGGKLLLDFIDKTGDRIHVFLE 383
           VGGKLLLDFIDKTGDRIHVFLE
Sbjct: 361 VGGKLLLDFIDKTGDRIHVFLE 382

BLAST of Moc05g08480 vs. NCBI nr
Match: XP_022138134.1 (uncharacterized protein LOC111009380 isoform X2 [Momordica charantia])

HSP 1 Score: 734.9 bits (1896), Expect = 3.4e-208
Identity = 381/382 (99.74%), Postives = 381/382 (99.74%), Query Frame = 0

Query: 1   MGEAPAKALRRILDSPGVHLGPACFDALSAKLIERAGFSYCFTSGFSISASRLALPDTGF 60
           MGEAPAKALRRILDSPGVHLGPACFDALSAKLIERAGFSYCFTSGFSISASRLALPDTGF
Sbjct: 1   MGEAPAKALRRILDSPGVHLGPACFDALSAKLIERAGFSYCFTSGFSISASRLALPDTGF 60

Query: 61  MSYGEMLDQGQQITEAVSIPVIGDGDNGYGNALNVKRTIKGFIRAGFAGILLEDQVSPKA 120
           MSYGEMLDQGQQITEAVSIPVIGDGDNGYGNALNVKRTIKGFIRAGFAGILLEDQVSPKA
Sbjct: 61  MSYGEMLDQGQQITEAVSIPVIGDGDNGYGNALNVKRTIKGFIRAGFAGILLEDQVSPKA 120

Query: 121 CGHTRGRKVVPREEAVSCIRAAVDARKESGSDIVIVARTDARQAVSLDEALWRTKAFADA 180
           CGHTRGRKVVPREEAVSCIRAAVDARKESGSDIVIVARTDARQAVSLDEALWRTKAFADA
Sbjct: 121 CGHTRGRKVVPREEAVSCIRAAVDARKESGSDIVIVARTDARQAVSLDEALWRTKAFADA 180

Query: 181 GADVLFIDALASIEEMKAFCKISPEVPKMANMLEGGGKTPILNPLELAEIGYKVVVYPLS 240
           GADVLFIDALASIEEMKAFCKISPEVPKMANMLEGGGKTPILNPLELAEIGYKVVVYPLS
Sbjct: 181 GADVLFIDALASIEEMKAFCKISPEVPKMANMLEGGGKTPILNPLELAEIGYKVVVYPLS 240

Query: 241 LVGISIRAMQDALLALKEGQIPSERMPSFEEMKEILGFNSYYEEEEKYASAVSLPSQGDG 300
           LVGISIRAMQDALLALKEGQIPSERMPSFEEMKEILGFNSYYEEEEKYASAVSLPSQ DG
Sbjct: 241 LVGISIRAMQDALLALKEGQIPSERMPSFEEMKEILGFNSYYEEEEKYASAVSLPSQ-DG 300

Query: 301 SKHPSSGILSRRLMVKITGKDGLEKLDVRIPAGSLDGVANIVPALGGVNIKELLNDAAEE 360
           SKHPSSGILSRRLMVKITGKDGLEKLDVRIPAGSLDGVANIVPALGGVNIKELLNDAAEE
Sbjct: 301 SKHPSSGILSRRLMVKITGKDGLEKLDVRIPAGSLDGVANIVPALGGVNIKELLNDAAEE 360

Query: 361 VGGKLLLDFIDKTGDRIHVFLE 383
           VGGKLLLDFIDKTGDRIHVFLE
Sbjct: 361 VGGKLLLDFIDKTGDRIHVFLE 381

BLAST of Moc05g08480 vs. NCBI nr
Match: KAG6582070.1 (2,3-dimethylmalate lyase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 674.5 bits (1739), Expect = 5.5e-190
Identity = 339/382 (88.74%), Postives = 362/382 (94.76%), Query Frame = 0

Query: 1   MGEAPAKALRRILDSPGVHLGPACFDALSAKLIERAGFSYCFTSGFSISASRLALPDTGF 60
           MGE PAKALR+ILDSPGVHLGPACFDALSAKLI+RAGFSYCFTSGFSISASRL LPDTGF
Sbjct: 228 MGETPAKALRKILDSPGVHLGPACFDALSAKLIQRAGFSYCFTSGFSISASRLGLPDTGF 287

Query: 61  MSYGEMLDQGQQITEAVSIPVIGDGDNGYGNALNVKRTIKGFIRAGFAGILLEDQVSPKA 120
           MSYGEMLDQG QIT+AVSIPVIGDGDNGYGNA+N+KRT+KG+IRAGFAGI+LEDQVSPKA
Sbjct: 288 MSYGEMLDQGLQITQAVSIPVIGDGDNGYGNAMNLKRTVKGYIRAGFAGIILEDQVSPKA 347

Query: 121 CGHTRGRKVVPREEAVSCIRAAVDARKESGSDIVIVARTDARQAVSLDEALWRTKAFADA 180
           CGHTRGRKVV REEAVSCIRAAVDARKESGSDIVIVARTDARQAVSLDEALWRTKAFADA
Sbjct: 348 CGHTRGRKVVSREEAVSCIRAAVDARKESGSDIVIVARTDARQAVSLDEALWRTKAFADA 407

Query: 181 GADVLFIDALASIEEMKAFCKISPEVPKMANMLEGGGKTPILNPLELAEIGYKVVVYPLS 240
           GAD+LFIDALAS+EEMKAFCK+SPEVPKMANMLEGGGKTPIL+PLELAEIGYKVVVYPLS
Sbjct: 408 GADILFIDALASVEEMKAFCKVSPEVPKMANMLEGGGKTPILSPLELAEIGYKVVVYPLS 467

Query: 241 LVGISIRAMQDALLALKEGQIPSERMPSFEEMKEILGFNSYYEEEEKYASAVSLPSQGDG 300
           L+G+S+RAMQDALLA+K GQIPSERMPSFEEMKEILGFNSYYEEEEKYA+A SLPS GDG
Sbjct: 468 LMGVSMRAMQDALLAIKGGQIPSERMPSFEEMKEILGFNSYYEEEEKYAAAASLPSPGDG 527

Query: 301 SKHPSSGILSRRLMVKITGKDGLEKLDVRIPAGSLDGVANIVPALGGVNIKELLNDAAEE 360
           SKHP S I  RRL VKITG DGLE+LD RIPAGSLD + +IVPALGG+NIK+LL+DAAEE
Sbjct: 528 SKHPPSDISPRRLRVKITGTDGLERLDARIPAGSLDTLTSIVPALGGINIKQLLDDAAEE 587

Query: 361 VGGKLLLDFIDKTGDRIHVFLE 383
           VGGK LLDF D TGDRI VFLE
Sbjct: 588 VGGKALLDFTDTTGDRIQVFLE 609

BLAST of Moc05g08480 vs. NCBI nr
Match: XP_023526814.1 (uncharacterized protein LOC111790200 [Cucurbita pepo subsp. pepo] >XP_023526816.1 uncharacterized protein LOC111790200 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 674.5 bits (1739), Expect = 5.5e-190
Identity = 338/382 (88.48%), Postives = 363/382 (95.03%), Query Frame = 0

Query: 1   MGEAPAKALRRILDSPGVHLGPACFDALSAKLIERAGFSYCFTSGFSISASRLALPDTGF 60
           MGE PAKALR+ILDSPGVHLGPACFDALSAKLI+RAGFSYCFTSGFSISASRL LPDTGF
Sbjct: 1   MGETPAKALRKILDSPGVHLGPACFDALSAKLIQRAGFSYCFTSGFSISASRLGLPDTGF 60

Query: 61  MSYGEMLDQGQQITEAVSIPVIGDGDNGYGNALNVKRTIKGFIRAGFAGILLEDQVSPKA 120
           MSYGEMLDQG QIT+AVSIPVIGDGDNGYGNA+N+KRT+KG+IRAGFAGI+LEDQVSPKA
Sbjct: 61  MSYGEMLDQGLQITQAVSIPVIGDGDNGYGNAMNLKRTVKGYIRAGFAGIILEDQVSPKA 120

Query: 121 CGHTRGRKVVPREEAVSCIRAAVDARKESGSDIVIVARTDARQAVSLDEALWRTKAFADA 180
           CGHTRGRKVV REEAVSCIRAAVDARKESGSDIVIVAR+DARQAVSLDEALWRTKAFADA
Sbjct: 121 CGHTRGRKVVSREEAVSCIRAAVDARKESGSDIVIVARSDARQAVSLDEALWRTKAFADA 180

Query: 181 GADVLFIDALASIEEMKAFCKISPEVPKMANMLEGGGKTPILNPLELAEIGYKVVVYPLS 240
           GAD+LFIDALAS+EEMKAFCK+SPEVPKMANMLEGGGKTPIL+PLELAEIGYKVVVYPLS
Sbjct: 181 GADILFIDALASVEEMKAFCKVSPEVPKMANMLEGGGKTPILSPLELAEIGYKVVVYPLS 240

Query: 241 LVGISIRAMQDALLALKEGQIPSERMPSFEEMKEILGFNSYYEEEEKYASAVSLPSQGDG 300
           L+G+S+RAMQDALLA+K GQIPSERMPSFEEMKEILGFNSYYEEEEKYA+A SLPS GDG
Sbjct: 241 LMGVSMRAMQDALLAIKGGQIPSERMPSFEEMKEILGFNSYYEEEEKYAAAASLPSPGDG 300

Query: 301 SKHPSSGILSRRLMVKITGKDGLEKLDVRIPAGSLDGVANIVPALGGVNIKELLNDAAEE 360
           SKHP S I  RRL VKITG DGLE+LD RIPAGSLD + NIVPALGG+NIK+L++DAAEE
Sbjct: 301 SKHPPSDISPRRLRVKITGTDGLERLDARIPAGSLDTLTNIVPALGGINIKKLIDDAAEE 360

Query: 361 VGGKLLLDFIDKTGDRIHVFLE 383
           VGGK+LLDF D TGDRI VFLE
Sbjct: 361 VGGKVLLDFTDTTGDRIQVFLE 382

BLAST of Moc05g08480 vs. NCBI nr
Match: XP_022979811.1 (uncharacterized protein LOC111479401 [Cucurbita maxima] >XP_022979812.1 uncharacterized protein LOC111479401 [Cucurbita maxima])

HSP 1 Score: 671.4 bits (1731), Expect = 4.6e-189
Identity = 337/382 (88.22%), Postives = 362/382 (94.76%), Query Frame = 0

Query: 1   MGEAPAKALRRILDSPGVHLGPACFDALSAKLIERAGFSYCFTSGFSISASRLALPDTGF 60
           MGE PAKALR+ILDSPGVHLGPACFDALSAKLI+RAGFSYCFTSGFSISASRL LPDTGF
Sbjct: 1   MGETPAKALRKILDSPGVHLGPACFDALSAKLIQRAGFSYCFTSGFSISASRLGLPDTGF 60

Query: 61  MSYGEMLDQGQQITEAVSIPVIGDGDNGYGNALNVKRTIKGFIRAGFAGILLEDQVSPKA 120
           MSYGEMLDQG QIT+AVSIPVIGDGDNGYGNA+N+KRT+KG+IRAGFAGI+LEDQVSPKA
Sbjct: 61  MSYGEMLDQGLQITQAVSIPVIGDGDNGYGNAMNLKRTVKGYIRAGFAGIILEDQVSPKA 120

Query: 121 CGHTRGRKVVPREEAVSCIRAAVDARKESGSDIVIVARTDARQAVSLDEALWRTKAFADA 180
           CGHTRGRKVV REEAVSCIRAA DARKESGSDIVIVARTDARQAVSLDEALWRTKAFADA
Sbjct: 121 CGHTRGRKVVSREEAVSCIRAAFDARKESGSDIVIVARTDARQAVSLDEALWRTKAFADA 180

Query: 181 GADVLFIDALASIEEMKAFCKISPEVPKMANMLEGGGKTPILNPLELAEIGYKVVVYPLS 240
           GAD+LFIDALAS+EEMKAFCK+SPEVPKMANMLEGGGKTPIL+PLELAEIGYKVVVYPLS
Sbjct: 181 GADILFIDALASVEEMKAFCKVSPEVPKMANMLEGGGKTPILSPLELAEIGYKVVVYPLS 240

Query: 241 LVGISIRAMQDALLALKEGQIPSERMPSFEEMKEILGFNSYYEEEEKYASAVSLPSQGDG 300
           L+G+S+RAMQDALLA+K GQIPSERMPSFEEMKEILGFNSYYEEEEKYA+A SLPS GDG
Sbjct: 241 LMGVSMRAMQDALLAIKGGQIPSERMPSFEEMKEILGFNSYYEEEEKYAAAASLPSPGDG 300

Query: 301 SKHPSSGILSRRLMVKITGKDGLEKLDVRIPAGSLDGVANIVPALGGVNIKELLNDAAEE 360
           SKHP S I +R+L VKITG DGLE+LD RIPAGSL  + NIVPALGG+NIK+LL+DAAEE
Sbjct: 301 SKHPPSDISARKLRVKITGTDGLERLDARIPAGSLATLTNIVPALGGINIKKLLDDAAEE 360

Query: 361 VGGKLLLDFIDKTGDRIHVFLE 383
           VGGK+LLDF D TGDRI VFLE
Sbjct: 361 VGGKVLLDFTDTTGDRIQVFLE 382

BLAST of Moc05g08480 vs. ExPASy Swiss-Prot
Match: Q0QLE4 (2,3-dimethylmalate lyase OS=Eubacterium barkeri OX=1528 GN=Dml PE=1 SV=1)

HSP 1 Score: 211.5 bits (537), Expect = 1.7e-53
Identity = 119/290 (41.03%), Postives = 176/290 (60.69%), Query Frame = 0

Query: 6   AKALRRILDSPGVHLGPACFDALSAKLIERAGFSYCFTSGFSISASRLALPDTGFMSYGE 65
           A  +R +L +  + + P   DA++AK+I R GF   + +G+  SAS L  PD G ++  E
Sbjct: 4   AAKMRELLSTKKMVVAPGAHDAMTAKVIGRLGFDAVYMTGYGQSASHLGQPDVGLLTMTE 63

Query: 66  MLDQGQQITEAVSIPVIGDGDNGYGNALNVKRTIKGFIRAGFAGILLEDQVSPKACGHTR 125
           M+ +   I EA  +PVI D D G+GNA+NV RT++ + +AG A I LEDQV PK CGH  
Sbjct: 64  MVARANAIVEAAGVPVIADADTGFGNAVNVMRTVREYEKAGVAVIQLEDQVMPKKCGHMV 123

Query: 126 GRKVVPREEAVSCIRAAVDARKESGSDIVIVARTDARQAVSLDEALWRTKAFADAGADVL 185
           GR++V +EE V  I+AAVD R     D +I+ARTDAR    +DEAL R  A+ +AGAD++
Sbjct: 124 GREIVSKEEMVGKIKAAVDTR--VNPDFMIMARTDARTTKGIDEALERGLAYKEAGADII 183

Query: 186 FIDALASIEEMKAFCKISPEVP--KMANMLEGGGKTPILNPLELAEIGYKVVVYPLSLVG 245
           FI++    EEMK   +I+  +P   +ANM+E GG+TP+L   EL  +GY + +YP + + 
Sbjct: 184 FIESPEGEEEMK---RINETIPGYTLANMVE-GGRTPLLKNAELEALGYNITIYPTASIY 243

Query: 246 ISIRAMQDALLALKEGQIPS---ERMPSFEEMKEILGFNSYYEEEEKYAS 291
           ++ +AM D   ALK     +   + M +F E  +++G     E E  YA+
Sbjct: 244 VATKAMVDLWTALKNDDTTAGVMDTMVTFSEFNDLMGLEKIREVEHNYAT 287

BLAST of Moc05g08480 vs. ExPASy Swiss-Prot
Match: O49290 (Carboxyvinyl-carboxyphosphonate phosphorylmutase, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=At1g77060 PE=1 SV=2)

HSP 1 Score: 198.4 bits (503), Expect = 1.5e-49
Identity = 115/287 (40.07%), Postives = 178/287 (62.02%), Query Frame = 0

Query: 11  RILDSPGVHLGPACFDALSAKLIERAGFSYCFTSGFSISASRLALPDTGFMSYGEMLDQG 70
           R+++  G+ L P C+DALSA ++++ GFS  F SG+++SAS L  PD G ++  EM    
Sbjct: 47  RLIEEQGIVLMPGCYDALSAAIVQQTGFSAGFISGYALSASLLGKPDFGLLTPPEMAATA 106

Query: 71  QQI-TEAVSIPVIGDGDNGYGNALNVKRTIKGFIRAGFAGILLEDQVSPKACGHTRGRKV 130
           + +   A +IP+I D D G GNALN++RT+K  I AG AG  LEDQ  PK CGH RG++V
Sbjct: 107 RSVCASAPNIPIIADADTGGGNALNIQRTVKDLIAAGAAGCFLEDQAWPKKCGHMRGKQV 166

Query: 131 VPREEAVSCIRAAVDARKESGSDIVIVARTDARQAVS---LDEALWRTKAFADAGADVLF 190
           +P EE  + I +A DA  +  SD  +VARTD R   +   L++A+ R   + +AGAD  F
Sbjct: 167 IPAEEHAAKIASARDAIGD--SDFFLVARTDVRATSAKSGLEDAIARVNLYMEAGADASF 226

Query: 191 IDALASIEEMKAFCKISPEVPKMANMLEGGGKTPILNPLELAEIGYKVVVYPLSLVGISI 250
           ++A    +E+K   K +    ++ NM+E GG TP+  P EL E+G+ ++V+PL+ +  S 
Sbjct: 227 VEAPRDDDELKEIGKRTKGY-RVCNMIE-GGVTPLHTPDELKEMGFHLIVHPLTALYAST 286

Query: 251 RAMQDALLALKEG---QIPSERMPSFEEMKEILGFNSYYEEEEKYAS 291
           RA+ D L  LKE    +   ++M +FEE   ++  +S++E E +Y++
Sbjct: 287 RALVDVLKTLKENGSTRDHLQKMATFEEFNSLVDLDSWFELEARYSN 329

BLAST of Moc05g08480 vs. ExPASy Swiss-Prot
Match: P11435 (Carboxyvinyl-carboxyphosphonate phosphorylmutase OS=Streptomyces hygroscopicus OX=1912 GN=bcpA PE=1 SV=3)

HSP 1 Score: 198.0 bits (502), Expect = 2.0e-49
Identity = 115/294 (39.12%), Postives = 170/294 (57.82%), Query Frame = 0

Query: 1   MGEAPAKALRRILDSPGVHLGPACFDALSAKLIERAGFSYCFTSGFSISASRLALPDTGF 60
           M    A+  R ++++P + + P+ +DALSAK+I++AGF     +G   SAS L LPD GF
Sbjct: 1   MAVTKARTFRELMNAPEILVVPSAYDALSAKVIQQAGFPAVHMTGSGTSASMLGLPDLGF 60

Query: 61  MSYGEMLDQGQQITEAVSIPVIGDGDNGYGNALNVKRTIKGFIRAGFAGILLEDQVSPKA 120
            S  E     + I   V +PVI D D GYGNA++V R  + F R G  G  LEDQV+PK 
Sbjct: 61  TSVSEQAINLKNIVLTVDVPVIMDADAGYGNAMSVWRATREFERVGIVGYHLEDQVNPKR 120

Query: 121 CGHTRGRKVVPREEAVSCIRAAVDARKESGSDIVIVARTDARQAVSLDEALWRTKAFADA 180
           CGH  G++++  EE    I AAV+AR++   D  I+ARTDAR++  LDEA+ R++ +  A
Sbjct: 121 CGHLEGKRLISTEEMTGKIEAAVEARED--EDFTIIARTDARESFGLDEAIRRSREYVAA 180

Query: 181 GADVLFIDALASIEEMKAFCKISPEVPKMANMLEGGGKTPILNPLELAEIGYKVVVYPLS 240
           GAD +F++A+  +EEMK   +   + P +ANM+E GGKTP L   EL  IGY + +YPLS
Sbjct: 181 GADCIFLEAMLDVEEMKR-VRDEIDAPLLANMVE-GGKTPWLTTKELESIGYNLAIYPLS 240

Query: 241 LVGISIRAMQDALLALKEGQIPSE------RMPSFEEMKEILGFNSYYEEEEKY 289
               +   ++     L+E     +         SF E+ E+  ++   E E ++
Sbjct: 241 GWMAAASVLRKLFTELREAGTTQKFWDDMGLKMSFAELFEVFEYSKISELEARF 290

BLAST of Moc05g08480 vs. ExPASy Swiss-Prot
Match: Q05957 (Petal death protein OS=Dianthus caryophyllus OX=3570 GN=PDP PE=1 SV=1)

HSP 1 Score: 191.4 bits (485), Expect = 1.8e-47
Identity = 113/284 (39.79%), Postives = 174/284 (61.27%), Query Frame = 0

Query: 9   LRRILDSPGVHLGPACFDALSAKLIERAGFSYCFTSGFSISASRLALPDTGFMSYGEMLD 68
           + R+++  G  L P   DALSA ++E+ GF   F SG+S+SA+ L LPD G ++  E+++
Sbjct: 31  MHRLIEEHGSVLMPGVQDALSAAVVEKTGFHAAFVSGYSVSAAMLGLPDFGLLTTTEVVE 90

Query: 69  QGQQITEAV-SIPVIGDGDNGYGNALNVKRTIKGFIRAGFAGILLEDQVSPKACGHTRGR 128
             ++IT A  ++ V+ DGD G G  LNV+R I+  I AG  G+ LEDQV PK CGH RG+
Sbjct: 91  ATRRITAAAPNLCVVVDGDTGGGGPLNVQRFIRELISAGAKGVFLEDQVWPKKCGHMRGK 150

Query: 129 KVVPREEAVSCIRAAVDARKESGSDIVIVARTDARQAVSLDEALWRTKAFADAGADVLFI 188
            VVP EE    I AA +A  +  SD  +VARTDAR    L+E + R   + +AGAD  F+
Sbjct: 151 AVVPAEEHALKIAAAREAIGD--SDFFLVARTDARAPHGLEEGIRRANLYKEAGADATFV 210

Query: 189 DALASIEEMKAFCKISPEVPKMANMLEGGGKTPILNPLELAEIGYKVVVYPLSLVGISIR 248
           +A A+++E+K     +  + ++ANM+E GGKTP+  P E  E+G+ ++ + L+ V  + R
Sbjct: 211 EAPANVDELKEVSAKTKGL-RIANMIE-GGKTPLHTPEEFKEMGFHLIAHSLTAVYATAR 270

Query: 249 AMQDALLALKE---GQIPSERMPSFEEMKEILGFNSYYEEEEKY 289
           A+ + +  LKE    +   ++M +F E  E++   S+YE E K+
Sbjct: 271 ALVNIMKILKEKGTTRDDLDQMATFSEFNELISLESWYEMESKF 310

BLAST of Moc05g08480 vs. ExPASy Swiss-Prot
Match: Q9YFM7 (2-methylisocitrate lyase OS=Aeropyrum pernix (strain ATCC 700893 / DSM 11879 / JCM 9820 / NBRC 100138 / K1) OX=272557 GN=prpB PE=3 SV=2)

HSP 1 Score: 179.5 bits (454), Expect = 7.2e-44
Identity = 109/307 (35.50%), Postives = 177/307 (57.65%), Query Frame = 0

Query: 3   EAPAKALRRILDSPGVHLGPACFDALSAKLIERAGFSYCFTSGFSISASRLALPDTGFMS 62
           E P   LR +++   + + P  ++   A L ER GF   + SG +I+ S LA+PD G ++
Sbjct: 10  ERPGLVLRELIEKRDIVVAPGVYNPAVALLAERMGFEALYLSGAAITGS-LAMPDLGLIT 69

Query: 63  YGEMLDQGQQITEAVSIPVIGDGDNGYGNALNVKRTIKGFIRAGFAGILLEDQVSPKACG 122
             E+      IT  V +PVI D D G+G A+NV+RT++   RAG A I +EDQV PK CG
Sbjct: 70  LSELAMFTSYITRVVRVPVIVDADTGFGEAINVERTVRELERAGAAAIQIEDQVMPKKCG 129

Query: 123 HTRGRKVVPREEAVSCIRAAVDARKESGSDIVIVARTDARQAVSLDEALWRTKAFADAGA 182
           H +G+ ++  E+ V  I AAV AR+    D +IVARTDAR     ++A+ R + + +AGA
Sbjct: 130 HLQGKALISPEDMVKKIIAAVGARR----DALIVARTDARGVEGFEKAVERAQLYVEAGA 189

Query: 183 DVLFIDALASIEEMKAFCKISPEVPKMANMLEGGGKTPILNPLELAEIGYKVVVYPLSLV 242
           D++F +AL S+EE + F +   + P +ANM E  GKTP +   +  E GYK+V++P++  
Sbjct: 190 DIIFPEALTSLEEFREFAR-RVKAPLLANMTE-FGKTPYITVDQFREAGYKIVIFPVTTF 249

Query: 243 GISIRAMQDALLALKEGQIPS---ERMPSFEEMKEILGFNSYYEEEEKYASAVSLPSQGD 302
             S++A +  L  + E        +++ +  E  +++G++ Y    EK  + VS  ++  
Sbjct: 250 RASLKASETVLREIMEKGTQKDILDKLYTRTEFYDLIGYHDY----EKRDAEVSRKAEEL 305

Query: 303 GSKHPSS 307
            ++H +S
Sbjct: 310 LARHNNS 305

BLAST of Moc05g08480 vs. ExPASy TrEMBL
Match: A0A6J1CC73 (Isocitrate lyase OS=Momordica charantia OX=3673 GN=LOC111009380 PE=4 SV=1)

HSP 1 Score: 741.9 bits (1914), Expect = 1.4e-210
Identity = 382/382 (100.00%), Postives = 382/382 (100.00%), Query Frame = 0

Query: 1   MGEAPAKALRRILDSPGVHLGPACFDALSAKLIERAGFSYCFTSGFSISASRLALPDTGF 60
           MGEAPAKALRRILDSPGVHLGPACFDALSAKLIERAGFSYCFTSGFSISASRLALPDTGF
Sbjct: 1   MGEAPAKALRRILDSPGVHLGPACFDALSAKLIERAGFSYCFTSGFSISASRLALPDTGF 60

Query: 61  MSYGEMLDQGQQITEAVSIPVIGDGDNGYGNALNVKRTIKGFIRAGFAGILLEDQVSPKA 120
           MSYGEMLDQGQQITEAVSIPVIGDGDNGYGNALNVKRTIKGFIRAGFAGILLEDQVSPKA
Sbjct: 61  MSYGEMLDQGQQITEAVSIPVIGDGDNGYGNALNVKRTIKGFIRAGFAGILLEDQVSPKA 120

Query: 121 CGHTRGRKVVPREEAVSCIRAAVDARKESGSDIVIVARTDARQAVSLDEALWRTKAFADA 180
           CGHTRGRKVVPREEAVSCIRAAVDARKESGSDIVIVARTDARQAVSLDEALWRTKAFADA
Sbjct: 121 CGHTRGRKVVPREEAVSCIRAAVDARKESGSDIVIVARTDARQAVSLDEALWRTKAFADA 180

Query: 181 GADVLFIDALASIEEMKAFCKISPEVPKMANMLEGGGKTPILNPLELAEIGYKVVVYPLS 240
           GADVLFIDALASIEEMKAFCKISPEVPKMANMLEGGGKTPILNPLELAEIGYKVVVYPLS
Sbjct: 181 GADVLFIDALASIEEMKAFCKISPEVPKMANMLEGGGKTPILNPLELAEIGYKVVVYPLS 240

Query: 241 LVGISIRAMQDALLALKEGQIPSERMPSFEEMKEILGFNSYYEEEEKYASAVSLPSQGDG 300
           LVGISIRAMQDALLALKEGQIPSERMPSFEEMKEILGFNSYYEEEEKYASAVSLPSQGDG
Sbjct: 241 LVGISIRAMQDALLALKEGQIPSERMPSFEEMKEILGFNSYYEEEEKYASAVSLPSQGDG 300

Query: 301 SKHPSSGILSRRLMVKITGKDGLEKLDVRIPAGSLDGVANIVPALGGVNIKELLNDAAEE 360
           SKHPSSGILSRRLMVKITGKDGLEKLDVRIPAGSLDGVANIVPALGGVNIKELLNDAAEE
Sbjct: 301 SKHPSSGILSRRLMVKITGKDGLEKLDVRIPAGSLDGVANIVPALGGVNIKELLNDAAEE 360

Query: 361 VGGKLLLDFIDKTGDRIHVFLE 383
           VGGKLLLDFIDKTGDRIHVFLE
Sbjct: 361 VGGKLLLDFIDKTGDRIHVFLE 382

BLAST of Moc05g08480 vs. ExPASy TrEMBL
Match: A0A6J1C976 (Isocitrate lyase OS=Momordica charantia OX=3673 GN=LOC111009380 PE=4 SV=1)

HSP 1 Score: 734.9 bits (1896), Expect = 1.7e-208
Identity = 381/382 (99.74%), Postives = 381/382 (99.74%), Query Frame = 0

Query: 1   MGEAPAKALRRILDSPGVHLGPACFDALSAKLIERAGFSYCFTSGFSISASRLALPDTGF 60
           MGEAPAKALRRILDSPGVHLGPACFDALSAKLIERAGFSYCFTSGFSISASRLALPDTGF
Sbjct: 1   MGEAPAKALRRILDSPGVHLGPACFDALSAKLIERAGFSYCFTSGFSISASRLALPDTGF 60

Query: 61  MSYGEMLDQGQQITEAVSIPVIGDGDNGYGNALNVKRTIKGFIRAGFAGILLEDQVSPKA 120
           MSYGEMLDQGQQITEAVSIPVIGDGDNGYGNALNVKRTIKGFIRAGFAGILLEDQVSPKA
Sbjct: 61  MSYGEMLDQGQQITEAVSIPVIGDGDNGYGNALNVKRTIKGFIRAGFAGILLEDQVSPKA 120

Query: 121 CGHTRGRKVVPREEAVSCIRAAVDARKESGSDIVIVARTDARQAVSLDEALWRTKAFADA 180
           CGHTRGRKVVPREEAVSCIRAAVDARKESGSDIVIVARTDARQAVSLDEALWRTKAFADA
Sbjct: 121 CGHTRGRKVVPREEAVSCIRAAVDARKESGSDIVIVARTDARQAVSLDEALWRTKAFADA 180

Query: 181 GADVLFIDALASIEEMKAFCKISPEVPKMANMLEGGGKTPILNPLELAEIGYKVVVYPLS 240
           GADVLFIDALASIEEMKAFCKISPEVPKMANMLEGGGKTPILNPLELAEIGYKVVVYPLS
Sbjct: 181 GADVLFIDALASIEEMKAFCKISPEVPKMANMLEGGGKTPILNPLELAEIGYKVVVYPLS 240

Query: 241 LVGISIRAMQDALLALKEGQIPSERMPSFEEMKEILGFNSYYEEEEKYASAVSLPSQGDG 300
           LVGISIRAMQDALLALKEGQIPSERMPSFEEMKEILGFNSYYEEEEKYASAVSLPSQ DG
Sbjct: 241 LVGISIRAMQDALLALKEGQIPSERMPSFEEMKEILGFNSYYEEEEKYASAVSLPSQ-DG 300

Query: 301 SKHPSSGILSRRLMVKITGKDGLEKLDVRIPAGSLDGVANIVPALGGVNIKELLNDAAEE 360
           SKHPSSGILSRRLMVKITGKDGLEKLDVRIPAGSLDGVANIVPALGGVNIKELLNDAAEE
Sbjct: 301 SKHPSSGILSRRLMVKITGKDGLEKLDVRIPAGSLDGVANIVPALGGVNIKELLNDAAEE 360

Query: 361 VGGKLLLDFIDKTGDRIHVFLE 383
           VGGKLLLDFIDKTGDRIHVFLE
Sbjct: 361 VGGKLLLDFIDKTGDRIHVFLE 381

BLAST of Moc05g08480 vs. ExPASy TrEMBL
Match: A0A6J1IPQ6 (Isocitrate lyase OS=Cucurbita maxima OX=3661 GN=LOC111479401 PE=4 SV=1)

HSP 1 Score: 671.4 bits (1731), Expect = 2.2e-189
Identity = 337/382 (88.22%), Postives = 362/382 (94.76%), Query Frame = 0

Query: 1   MGEAPAKALRRILDSPGVHLGPACFDALSAKLIERAGFSYCFTSGFSISASRLALPDTGF 60
           MGE PAKALR+ILDSPGVHLGPACFDALSAKLI+RAGFSYCFTSGFSISASRL LPDTGF
Sbjct: 1   MGETPAKALRKILDSPGVHLGPACFDALSAKLIQRAGFSYCFTSGFSISASRLGLPDTGF 60

Query: 61  MSYGEMLDQGQQITEAVSIPVIGDGDNGYGNALNVKRTIKGFIRAGFAGILLEDQVSPKA 120
           MSYGEMLDQG QIT+AVSIPVIGDGDNGYGNA+N+KRT+KG+IRAGFAGI+LEDQVSPKA
Sbjct: 61  MSYGEMLDQGLQITQAVSIPVIGDGDNGYGNAMNLKRTVKGYIRAGFAGIILEDQVSPKA 120

Query: 121 CGHTRGRKVVPREEAVSCIRAAVDARKESGSDIVIVARTDARQAVSLDEALWRTKAFADA 180
           CGHTRGRKVV REEAVSCIRAA DARKESGSDIVIVARTDARQAVSLDEALWRTKAFADA
Sbjct: 121 CGHTRGRKVVSREEAVSCIRAAFDARKESGSDIVIVARTDARQAVSLDEALWRTKAFADA 180

Query: 181 GADVLFIDALASIEEMKAFCKISPEVPKMANMLEGGGKTPILNPLELAEIGYKVVVYPLS 240
           GAD+LFIDALAS+EEMKAFCK+SPEVPKMANMLEGGGKTPIL+PLELAEIGYKVVVYPLS
Sbjct: 181 GADILFIDALASVEEMKAFCKVSPEVPKMANMLEGGGKTPILSPLELAEIGYKVVVYPLS 240

Query: 241 LVGISIRAMQDALLALKEGQIPSERMPSFEEMKEILGFNSYYEEEEKYASAVSLPSQGDG 300
           L+G+S+RAMQDALLA+K GQIPSERMPSFEEMKEILGFNSYYEEEEKYA+A SLPS GDG
Sbjct: 241 LMGVSMRAMQDALLAIKGGQIPSERMPSFEEMKEILGFNSYYEEEEKYAAAASLPSPGDG 300

Query: 301 SKHPSSGILSRRLMVKITGKDGLEKLDVRIPAGSLDGVANIVPALGGVNIKELLNDAAEE 360
           SKHP S I +R+L VKITG DGLE+LD RIPAGSL  + NIVPALGG+NIK+LL+DAAEE
Sbjct: 301 SKHPPSDISARKLRVKITGTDGLERLDARIPAGSLATLTNIVPALGGINIKKLLDDAAEE 360

Query: 361 VGGKLLLDFIDKTGDRIHVFLE 383
           VGGK+LLDF D TGDRI VFLE
Sbjct: 361 VGGKVLLDFTDTTGDRIQVFLE 382

BLAST of Moc05g08480 vs. ExPASy TrEMBL
Match: A0A6J1GVA0 (Isocitrate lyase OS=Cucurbita moschata OX=3662 GN=LOC111457862 PE=4 SV=1)

HSP 1 Score: 669.5 bits (1726), Expect = 8.5e-189
Identity = 336/382 (87.96%), Postives = 361/382 (94.50%), Query Frame = 0

Query: 1   MGEAPAKALRRILDSPGVHLGPACFDALSAKLIERAGFSYCFTSGFSISASRLALPDTGF 60
           MGE PAKALR+ILDSPGVHLGPACFDALSAKLI+RAGFSYCFTSGFSISASRL LPDTGF
Sbjct: 1   MGETPAKALRKILDSPGVHLGPACFDALSAKLIQRAGFSYCFTSGFSISASRLGLPDTGF 60

Query: 61  MSYGEMLDQGQQITEAVSIPVIGDGDNGYGNALNVKRTIKGFIRAGFAGILLEDQVSPKA 120
           MSYGEMLDQG QIT+AVSIPVIGDGDNGYGNA+N+KRT+KG+IRAGFAGI+LEDQVSPKA
Sbjct: 61  MSYGEMLDQGLQITQAVSIPVIGDGDNGYGNAMNLKRTVKGYIRAGFAGIILEDQVSPKA 120

Query: 121 CGHTRGRKVVPREEAVSCIRAAVDARKESGSDIVIVARTDARQAVSLDEALWRTKAFADA 180
           CGHTRGRKVV REEAVSCIRAAVDARKESG DIVIVARTDARQAVSLDEALWRTKAFADA
Sbjct: 121 CGHTRGRKVVSREEAVSCIRAAVDARKESGYDIVIVARTDARQAVSLDEALWRTKAFADA 180

Query: 181 GADVLFIDALASIEEMKAFCKISPEVPKMANMLEGGGKTPILNPLELAEIGYKVVVYPLS 240
           GAD+LFIDALAS+EEMKAFCK+SPEVPKMANMLEGGGKTPIL+PLELAEIGYKVVVYPLS
Sbjct: 181 GADILFIDALASVEEMKAFCKVSPEVPKMANMLEGGGKTPILSPLELAEIGYKVVVYPLS 240

Query: 241 LVGISIRAMQDALLALKEGQIPSERMPSFEEMKEILGFNSYYEEEEKYASAVSLPSQGDG 300
           L+G+S+RAMQDALLA+K GQIPSERMPSFEEMKEILGFNSYYEEE+KYA+A SLPS GDG
Sbjct: 241 LMGVSMRAMQDALLAIKGGQIPSERMPSFEEMKEILGFNSYYEEEDKYAAAASLPSPGDG 300

Query: 301 SKHPSSGILSRRLMVKITGKDGLEKLDVRIPAGSLDGVANIVPALGGVNIKELLNDAAEE 360
           SKHP S I  RRL VKITG DGLE+LD RIPAGSLD + +IVPALGG+NIK+LL+DAAEE
Sbjct: 301 SKHPPSDISPRRLRVKITGTDGLERLDARIPAGSLDTLTSIVPALGGINIKQLLDDAAEE 360

Query: 361 VGGKLLLDFIDKTGDRIHVFLE 383
           VGGK+LLDF D T DRI VFLE
Sbjct: 361 VGGKVLLDFTDTTSDRIQVFLE 382

BLAST of Moc05g08480 vs. ExPASy TrEMBL
Match: A0A6J1GWR7 (Isocitrate lyase OS=Cucurbita moschata OX=3662 GN=LOC111457859 PE=4 SV=1)

HSP 1 Score: 598.2 bits (1541), Expect = 2.4e-167
Identity = 314/424 (74.06%), Postives = 348/424 (82.08%), Query Frame = 0

Query: 1   MGEAPAKALRRILDSPGVHLGPACFDALSAKLIERAGFSYCFTSGFSISASRLALPDTGF 60
           M E+PAKALRRIL+ PGVH GPACFDALSAKL+ERAGF YCFTSGFSISA+RLALPDTGF
Sbjct: 74  MAESPAKALRRILEMPGVHQGPACFDALSAKLVERAGFLYCFTSGFSISAARLALPDTGF 133

Query: 61  MSYGEMLDQGQQITEAVSIPVIGDGDNGYGNALNVKRTIKGFIRAGFAGILLEDQVSPKA 120
           +SYGEMLDQGQ IT++VSIPVIGDGDNGYGNA+NVKRT+KG+IRAGFAGI+LEDQVSPKA
Sbjct: 134 ISYGEMLDQGQLITQSVSIPVIGDGDNGYGNAMNVKRTVKGYIRAGFAGIILEDQVSPKA 193

Query: 121 CGHTRGRKVVPREEAVSCIRAAVDARKESGSDIVIVARTDARQAVSLDEALWRTKAFADA 180
           CGHT+GRKVV REEAV  I+AAVDARKESGSDIVIVARTD+RQAVSL E+LWR +AFADA
Sbjct: 194 CGHTQGRKVVSREEAVLRIKAAVDARKESGSDIVIVARTDSRQAVSLSESLWRARAFADA 253

Query: 181 GADVLFIDALASIEEMKAFCKISPEVPKMANMLEGGGKTPILNPLELAEIGYKVVVYPLS 240
           GADVLFIDALAS +EM+AFCKISP++PKMANMLEGGGKTPI NPLEL EIGYK+V YPLS
Sbjct: 254 GADVLFIDALASKDEMEAFCKISPKIPKMANMLEGGGKTPIFNPLELEEIGYKLVAYPLS 313

Query: 241 LVGISIRAMQDALLALKEGQIPSE-RMPSFEEMKEILGFNSYYEEEEKYASAVSLPS--- 300
           LVG+SIRAMQDALLA+K G++PS   +P+F EMKEILGFNSYYEEE KYASAVS PS   
Sbjct: 314 LVGVSIRAMQDALLAIKGGRLPSPGTLPTFGEMKEILGFNSYYEEERKYASAVSQPSIKV 373

Query: 301 --------------------------------------QGDGSKHPSSGILSRRLMVKIT 360
                                                   D SK P SGI SRRL VKIT
Sbjct: 374 ESNISSLQRRVEDDSEKKDENPQGPVVEVITPEIYRSYDADESKGPFSGIWSRRLRVKIT 433

Query: 361 GKDGLEKLDVRIPAGSLDGVANIVPALGGVNIKELLNDAAEEVGGKLLLDFIDKTGDRIH 383
           G+DG E+LDVRIPAG L+G+ NIVPALGGVNIKEL++DAA EVGGKLLLDF+D  GDRI 
Sbjct: 434 GRDGFERLDVRIPAGFLEGLTNIVPALGGVNIKELMDDAAGEVGGKLLLDFVDGMGDRIE 493

BLAST of Moc05g08480 vs. TAIR 10
Match: AT2G43180.3 (Phosphoenolpyruvate carboxylase family protein )

HSP 1 Score: 485.3 bits (1248), Expect = 4.4e-137
Identity = 258/411 (62.77%), Postives = 314/411 (76.40%), Query Frame = 0

Query: 4   APAKALRRILDSPGVHLGPACFDALSAKLIERAGFSYCFTSGFSISASRLALPDTGFMSY 63
           +PAK LR I+ SPGV  GP CFDALSAKLIERAGF YC TSGFSISASRL LPD G +SY
Sbjct: 71  SPAKKLRNIMQSPGVLQGPCCFDALSAKLIERAGFPYCITSGFSISASRLGLPDKGLISY 130

Query: 64  GEMLDQGQQITEAVSIPVIGDGDNGYGNALNVKRTIKGFIRAGFAGILLEDQVSPKACGH 123
           GEM+DQGQQIT++VSIPVIGDG NGYGNA+NVKRT+KG+I+AGFAGI++ D+V    C +
Sbjct: 131 GEMVDQGQQITQSVSIPVIGDGGNGYGNAMNVKRTVKGYIKAGFAGIIINDEV---CCEN 190

Query: 124 TRG-RKVVPREEAVSCIRAAVDARKESGSDIVIVARTDARQAVSLDEALWRTKAFADAGA 183
           T+  R+VV REEAV  ++AAVDAR+E  SDIVIVA+TD+R+A+SL+E+L R +AF DAGA
Sbjct: 191 TKSERRVVSREEAVMRVKAAVDARRECDSDIVIVAQTDSREAISLEESLIRARAFTDAGA 250

Query: 184 DVLFIDALASIEEMKAFCKISPEVPKMANMLEGGGKTPILNPLELAEIGYKVVVYPLSLV 243
           DVL +D+L S EEMKAFC + P VPK+ANMLE GGK PILNPLEL EIGYK+V YP+SL+
Sbjct: 251 DVLSVDSLVSREEMKAFCNVYPLVPKLANMLESGGKIPILNPLELEEIGYKLVAYPISLI 310

Query: 244 GISIRAMQDALLALKEGQIPSE-RMPSFEEMKEILGFNSYYEEEEKYASA---------- 303
           G+SI+AMQDALLA+K G+IP    M S EE+ EILGF++Y EEE++YA++          
Sbjct: 311 GVSIQAMQDALLAIKGGRIPPPGSMASLEEIGEILGFDTYEEEEKRYATSSSDRVSSSSV 370

Query: 304 --------------------VSLPSQGDGSKHPSSGILSRRLMVKITGKDGLEKLDVRIP 363
                               V  P   +  ++P S I SR L +KI G+DG EKLDVRIP
Sbjct: 371 YRNQRVAKDDPEQREDLIVEVITPEVYNEPRNPFSRIWSRSLRIKIIGRDGFEKLDVRIP 430

Query: 364 AGSLDGVANIVPALGGVNIKELLNDAAEEVGGKLLLDFIDKTGDRIHVFLE 383
           AG L+GV NIVPALGGVN+K+L++DAA+EVGGK+LLDF D  GDRI VFLE
Sbjct: 431 AGFLEGVTNIVPALGGVNLKQLMDDAADEVGGKILLDFKDTAGDRIQVFLE 478

BLAST of Moc05g08480 vs. TAIR 10
Match: AT2G43180.1 (Phosphoenolpyruvate carboxylase family protein )

HSP 1 Score: 485.0 bits (1247), Expect = 5.7e-137
Identity = 258/412 (62.62%), Postives = 314/412 (76.21%), Query Frame = 0

Query: 4   APAKALRRILDSPGVHLGPACFDALSAKLIERAGFSYCFTSGFSISASRLALPDTGFMSY 63
           +PAK LR I+ SPGV  GP CFDALSAKLIERAGF YC TSGFSISASRL LPD G +SY
Sbjct: 71  SPAKKLRNIMQSPGVLQGPCCFDALSAKLIERAGFPYCITSGFSISASRLGLPDKGLISY 130

Query: 64  GEMLDQGQQITEAVSIPVIGDGDNGYGNALNVKRTIKGFIRAGFAGILLEDQVSPKACGH 123
           GEM+DQGQQIT++VSIPVIGDG NGYGNA+NVKRT+KG+I+AGFAGI++ D+V    C +
Sbjct: 131 GEMVDQGQQITQSVSIPVIGDGGNGYGNAMNVKRTVKGYIKAGFAGIIINDEV---CCEN 190

Query: 124 TRG-RKVVPREEAVSCIRAAVDARKESGSDIVIVARTDARQAVSLDEALWRTKAFADAGA 183
           T+  R+VV REEAV  ++AAVDAR+E  SDIVIVA+TD+R+A+SL+E+L R +AF DAGA
Sbjct: 191 TKSERRVVSREEAVMRVKAAVDARRECDSDIVIVAQTDSREAISLEESLIRARAFTDAGA 250

Query: 184 DVLFIDALASIEEMKAFCKISPEVPKMANMLEGGGKTPILNPLELAEIGYKVVVYPLSLV 243
           DVL +D+L S EEMKAFC + P VPK+ANMLE GGK PILNPLEL EIGYK+V YP+SL+
Sbjct: 251 DVLSVDSLVSREEMKAFCNVYPLVPKLANMLESGGKIPILNPLELEEIGYKLVAYPISLI 310

Query: 244 GISIRAMQDALLALKEGQIPSE-RMPSFEEMKEILGFNSYYEEEEKYASA---------- 303
           G+SI+AMQDALLA+K G+IP    M S EE+ EILGF++Y EEE++YA++          
Sbjct: 311 GVSIQAMQDALLAIKGGRIPPPGSMASLEEIGEILGFDTYEEEEKRYATSSSDREVSSSS 370

Query: 304 ---------------------VSLPSQGDGSKHPSSGILSRRLMVKITGKDGLEKLDVRI 363
                                V  P   +  ++P S I SR L +KI G+DG EKLDVRI
Sbjct: 371 VYRNQRVAKDDPEQREDLIVEVITPEVYNEPRNPFSRIWSRSLRIKIIGRDGFEKLDVRI 430

Query: 364 PAGSLDGVANIVPALGGVNIKELLNDAAEEVGGKLLLDFIDKTGDRIHVFLE 383
           PAG L+GV NIVPALGGVN+K+L++DAA+EVGGK+LLDF D  GDRI VFLE
Sbjct: 431 PAGFLEGVTNIVPALGGVNLKQLMDDAADEVGGKILLDFKDTAGDRIQVFLE 479

BLAST of Moc05g08480 vs. TAIR 10
Match: AT2G43180.2 (Phosphoenolpyruvate carboxylase family protein )

HSP 1 Score: 426.4 bits (1095), Expect = 2.4e-119
Identity = 229/375 (61.07%), Postives = 280/375 (74.67%), Query Frame = 0

Query: 4   APAKALRRILDSPGVHLGPACFDALSAKLIERAGFSYCFTSGFSISASRLALPDTGFMSY 63
           +PAK LR I+ SPGV  GP CFDALSAKLIERAGF YC TSGFSISASRL LPD G +SY
Sbjct: 71  SPAKKLRNIMQSPGVLQGPCCFDALSAKLIERAGFPYCITSGFSISASRLGLPDKGLISY 130

Query: 64  GEMLDQGQQITEAVSIPVIGDGDNGYGNALNVKRTIKGFIRAGFAGILLEDQVSPKACGH 123
           GEM+DQGQQIT++VSIPVIGDG NGYGNA+NVKRT+KG+I+AGFAGI++ D+V    C +
Sbjct: 131 GEMVDQGQQITQSVSIPVIGDGGNGYGNAMNVKRTVKGYIKAGFAGIIINDEV---CCEN 190

Query: 124 TRG-RKVVPREEAVSCIRAAVDARKESGSDIVIVARTDARQAVSLDEALWRTKAFADAGA 183
           T+  R+VV REEAV  ++AAVDAR+E  SDIVIVA+TD+R+A+SL+E+L R +AF DAGA
Sbjct: 191 TKSERRVVSREEAVMRVKAAVDARRECDSDIVIVAQTDSREAISLEESLIRARAFTDAGA 250

Query: 184 DVLFIDALASIEEMKAFCKISPEVPKMANMLEGGGKTPILNPLELAEIGYKVVVYPLSLV 243
           DVL +D+L S EEMKAFC + P VPK+ANMLE GGK PILNPLEL EIGYK+V YP+SL+
Sbjct: 251 DVLSVDSLVSREEMKAFCNVYPLVPKLANMLESGGKIPILNPLELEEIGYKLVAYPISLI 310

Query: 244 GISIRAMQDALLALKEGQIPSE-RMPSFEEMKEILGFNSYYEEEEKYASA---------- 303
           G+SI+AMQDALLA+K G+IP    M S EE+ EILGF++Y EEE++YA++          
Sbjct: 311 GVSIQAMQDALLAIKGGRIPPPGSMASLEEIGEILGFDTYEEEEKRYATSSSDREVSSSS 370

Query: 304 ---------------------VSLPSQGDGSKHPSSGILSRRLMVKITGKDGLEKLDVRI 346
                                V  P   +  ++P S I SR L +KI G+DG EKLDVRI
Sbjct: 371 VYRNQRVAKDDPEQREDLIVEVITPEVYNEPRNPFSRIWSRSLRIKIIGRDGFEKLDVRI 430

BLAST of Moc05g08480 vs. TAIR 10
Match: AT2G43180.4 (Phosphoenolpyruvate carboxylase family protein )

HSP 1 Score: 409.8 bits (1052), Expect = 2.3e-114
Identity = 221/365 (60.55%), Postives = 270/365 (73.97%), Query Frame = 0

Query: 4   APAKALRRILDSPGVHLGPACFDALSAKLIERAGFSYCFTSGFSISASRLALPDTGFMSY 63
           +PAK LR I+ SPGV  GP CFDALSAKLIERAGF YC TSGFSISASRL LPD G +SY
Sbjct: 71  SPAKKLRNIMQSPGVLQGPCCFDALSAKLIERAGFPYCITSGFSISASRLGLPDKGLISY 130

Query: 64  GEMLDQGQQITEAVSIPVIGDGDNGYGNALNVKRTIKGFIRAGFAGILLEDQVSPKACGH 123
           GEM+DQGQQIT++VSIPVIGDG NGYGNA+NVKRT+KG+I+AGFAGI++ D+V    C +
Sbjct: 131 GEMVDQGQQITQSVSIPVIGDGGNGYGNAMNVKRTVKGYIKAGFAGIIINDEV---CCEN 190

Query: 124 TRG-RKVVPREEAVSCIRAAVDARKESGSDIVIVARTDARQAVSLDEALWRTKAFADAGA 183
           T+  R+VV REEAV  ++AAVDAR+E  SDIVIVA+TD+R+A+SL+E+L R +AF DAGA
Sbjct: 191 TKSERRVVSREEAVMRVKAAVDARRECDSDIVIVAQTDSREAISLEESLIRARAFTDAGA 250

Query: 184 DVLFIDALASIEEMKAFCKISPEVPKMANMLEGGGKTPILNPLELAEIGYKVVVYPLSLV 243
           DVL +D+L S EEMKAFC + P VPK+ANMLE GGK PILNPLEL EIGYK+V YP+SL+
Sbjct: 251 DVLSVDSLVSREEMKAFCNVYPLVPKLANMLESGGKIPILNPLELEEIGYKLVAYPISLI 310

Query: 244 GISIRAMQDALLALKEGQIPSE-RMPSFEEMKEILGFNSYYEEEEKYASA---------- 303
           G+SI+AMQDALLA+K G+IP    M S EE+ EILGF++Y EEE++YA++          
Sbjct: 311 GVSIQAMQDALLAIKGGRIPPPGSMASLEEIGEILGFDTYEEEEKRYATSSSDREVSSSS 370

Query: 304 ---------------------VSLPSQGDGSKHPSSGILSRRLMVKITGKDGLEKLDVRI 336
                                V  P   +  ++P S I SR L +KI G+DG EKLDVRI
Sbjct: 371 VYRNQRVAKDDPEQREDLIVEVITPEVYNEPRNPFSRIWSRSLRIKIIGRDGFEKLDVRI 430

BLAST of Moc05g08480 vs. TAIR 10
Match: AT1G77060.1 (Phosphoenolpyruvate carboxylase family protein )

HSP 1 Score: 198.4 bits (503), Expect = 1.1e-50
Identity = 115/287 (40.07%), Postives = 178/287 (62.02%), Query Frame = 0

Query: 11  RILDSPGVHLGPACFDALSAKLIERAGFSYCFTSGFSISASRLALPDTGFMSYGEMLDQG 70
           R+++  G+ L P C+DALSA ++++ GFS  F SG+++SAS L  PD G ++  EM    
Sbjct: 47  RLIEEQGIVLMPGCYDALSAAIVQQTGFSAGFISGYALSASLLGKPDFGLLTPPEMAATA 106

Query: 71  QQI-TEAVSIPVIGDGDNGYGNALNVKRTIKGFIRAGFAGILLEDQVSPKACGHTRGRKV 130
           + +   A +IP+I D D G GNALN++RT+K  I AG AG  LEDQ  PK CGH RG++V
Sbjct: 107 RSVCASAPNIPIIADADTGGGNALNIQRTVKDLIAAGAAGCFLEDQAWPKKCGHMRGKQV 166

Query: 131 VPREEAVSCIRAAVDARKESGSDIVIVARTDARQAVS---LDEALWRTKAFADAGADVLF 190
           +P EE  + I +A DA  +  SD  +VARTD R   +   L++A+ R   + +AGAD  F
Sbjct: 167 IPAEEHAAKIASARDAIGD--SDFFLVARTDVRATSAKSGLEDAIARVNLYMEAGADASF 226

Query: 191 IDALASIEEMKAFCKISPEVPKMANMLEGGGKTPILNPLELAEIGYKVVVYPLSLVGISI 250
           ++A    +E+K   K +    ++ NM+E GG TP+  P EL E+G+ ++V+PL+ +  S 
Sbjct: 227 VEAPRDDDELKEIGKRTKGY-RVCNMIE-GGVTPLHTPDELKEMGFHLIVHPLTALYAST 286

Query: 251 RAMQDALLALKEG---QIPSERMPSFEEMKEILGFNSYYEEEEKYAS 291
           RA+ D L  LKE    +   ++M +FEE   ++  +S++E E +Y++
Sbjct: 287 RALVDVLKTLKENGSTRDHLQKMATFEEFNSLVDLDSWFELEARYSN 329