Moc05g06050 (gene) Bitter gourd (OHB3-1) v2

Overview
NameMoc05g06050
Typegene
OrganismMomordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
DescriptionATP-dependent (S)-NAD(P)H-hydrate dehydratase
Locationchr5: 4171273 .. 4175512 (+)
RNA-Seq ExpressionMoc05g06050
SyntenyMoc05g06050
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAGTGGGACTTCTGTTGAGGCTGATGCTGAGAACATTATAAGAGCAATTACACCTTCTCTTGATCCCAACAGATACAAAGGCCAGGCGGGTAAGCTCAAAATGTACCCTGTAGTTCTATGGCTTTTCTTTTATTTTCCATCTGCGCAAATGGTTGGTAAATACTCGAGTATATTTTTTGTGATGAATCAGTTTGCAAGAACTTTATAGTTAGTCAACTTTTCAAATTGATGGTTTTTGGCTATTGGATAGATTTTGCTAACTCATTTTCAATATTGATCCCTGGTGTCAGTTTACGCTTTTGTCTTCCAATTACGTTTTATGCTTTATTTTATGATTTTCCCTGTGTTAGAATTAGAGTGTGGCTAAGGTTGAGAAATACTCTATTTAACAATCTATATATGTTTATCAGGGAAGATAGCTGTTATTGGTGGATGTCGTGAGTACACAGGTGCTCCATACTTCGCTGCTATATCAGCTTTGAAAATTGTTAGTTCAGACTCTTTTTCTTGGTTGTCTTGTTAGAGAATTTTATTCTACATTTTTGTTTATATAATTAATTTTTCCTCTTTTTACTCGTGAAGGTAAGGTGTATAACAAACAAACCATTTGTTTTTCCTTTCAGGGTGCAGATTTGTCTCACGTTTTCTGCACAAAAGATGCTGCTCCTGTCATAAAGAGCTATAGTCCAGAGTTAATTGTGCATCCCGTTTTAGAAGAATCATACAGCGTCAGGTGCTTTTTGCTTTCTATATTTCGGTCCGAGGGAAAAAAAAGAATTAAATGGAAATTTTAATATTTTTCTAATGCCTCTAATGATGTTTTAGAGATGAGGAAAAGAAGTTCATTGCAGAAAAGGTTCTTGCTGAGGTTGACAAGTGGCTGGAAAGATTTGATTGCCTTGTCATTGGTCCAGGCCTCGGAAGGGACCCATTTCTTCTGGTGATTTTGTTATTGCTTTTTGGCTAAATTATATAATTTGCTCTTGAACTATGGACTTTATTTTAAAAGCTTTAATGATATCTTCAAGTTTTCAACCTTTTGGCTGACATGTTTGAGAAAATGAGATTTTTCATATTGATTTGGCAAATGAGATAGTGAAAGCCCTCTCCTTTTTTCCTTCTTCATGCTAACTGCTGAATGAATGAAGGTAAAATCCTTCTTTACTGTAGTCCTTTTGCCACATTGGGACGAAAACCTCATTTTTGTTGTACACGTCAGTCAGTAGTTTTAAAAATAACCATTCATGTCCATTTCTCATTGTTTTTTAGTGGTTGGGAGTCTTAATTTAAATCAATCAAAAGTATGGGGGTAGGTAGTTGAGAATTTGAAAACTTAAGGAACAAATTTGAAATCAAGTGCATAGTTAGGGAAGGATGCTAGAACTTTTCCCATGTCTTAATTCTTTCGAGTAGATTGAACTAAGATGCATAGTTCGATAACGATTGTATTTTCTTATCTGAAAATGATTGTCAAGTCATATTGTTACCCAAACTTGATATGCATAAGGTACTCCCAGATGCCTTGCAAACAGAACTTCAGCAGCGTCTCTGACTATTACTTGTAATTATTGAGCTGCCCCTGCTTCAAATGATAGACTTTGAAAGTGCAGATTAGTTGGTTCTATTATAGGCTAAGGACAATGCCTCAAGATCATTAGAACTAGTAAGTCTGATGATAATTTGTTTCAAATAATCATTTTGGACAAGCTGATTAGGGCTATATATGCTAACTTGGCGTAAATTCTACAAATGGTTTCAATACCAGGGGCTGGTAACTTGATACCCCTACAATTATTTGTTCAAAGGATTTTAGGTGCGAAAATCAAATCAATTGAGATCCCAAAGATAGATACAAAAGATGTAAGAAATTCCACTCTCTGAGTCTTTACAAAAAATGGAAAAATTTTGATTCTCTGTGTCTCTCTTTTGCCTTTTCAGACCACTAAAATGGTAAAATGAAGTTAATTACTCCACTGTTTCTTCAATTACATTGGTCTGATATTTTCTGGCGGTTGCTTTGGCTGTAAGCCTATGCCTTTTTACTTTTTCAACAAGTCTTCAAACATGAATTATAATCATCCCAGAGGTTGAAGTGATTTTTCAAATCTAACAACTTTCTCCTTTGGATTTGTCATCAAATTTCTTTGTCTTGCCTATGAAACGCTACCATAAAATTTTGCTAATGGTTGAAAACTGTTGCAGGATTGTGTCAGCGAAATCATAAAGCATGCAAGGCAGACCAATATACCAATGGTTATAGACGGGGTATGCATATACCAAAGCAAATACGATTTTGCCTTGTTTATACTATTGTTATGCATGCTACTATCCAACAGGTCTCTAGGAGGTGGCTGCTGACAGCTGGTTGTGTTTGTTGCTTTTCAGGATGGACTCTTTCTTATCACTGACAATCTTCACCTTGTAAGCAATTACCCCCTGGCCGTCCTAACTCCAAATGTAAACGAATATAAACGTCTTGTGAAAAATGTGCTGCTTGCTGAAATCGATGAACAAGATGCTTCTAAGCAATTGCTGTCCCTCGTCAAAAGGTAAAGTCTTCATGTTTTCCCATCTGAATAGAGTTTCCATATGTCATGATTCTGGAATGGTGACGTACTTATTGCTCAAGTGAATCTTGTATGGACACACTACCTAGCGCAACCCCAAAATCAGCGGGTTAAGGTACATATATTTACAAAAGTTGGAAGCTGAGGGGTTGACCTCATCTGATCAGAATTCAGAATAGATGTTACTACATATTCAGGACTCATTATCGTTTTTTCTAAATGAAGTAACTCTATTTGCACATTCTGAAGTCTTTAGCCAAATGGTTCAATGTCAAGAATGGGAATGTTTTGGTGGTGTGTCCTACCCTTCCAACAGTTGATCACTCCTCGATTTAGCCTACTTCTTTCCTGAAATAATTATTTATGAAGTATGAAGGTACTAACATTAGGATGAAAATTATAACTGTATCTTACTAAAGGGATTCTTGTTGGCTAGCTATAAATAATCATCTGGCATTCTATATTGCCAAAGATAATTGGAGATAGCTTTTCCTACGCGGGTTTATGCTCCATTCTATTTTATAATTTCTTACATAGTGTACATTATTATTTAATTTAATTTTCCAGTCTTTGGTTCTCTAAACTTTTGGACATGATCAGGATTGGTGGCCTAACTATACTCAGGAAAGGGAGAGCTGACCTCATCAGTGACGGTGAGACAGGTACCGAGTACAACTCCAAAATAATTTTCCATGTCAATGTTGTAAAAGTGCTACATTCAATGTTTCTTCTCTATGATTAGGAGATTAATTGATTGTCAGTGTCACCAACCAGAAGTTTACCTCCTGGTCAGATTAGGAAAAAAACAATAAAATTCCCTCTTATGTATACTAAGCTAAGCTGTTGTCCTGATATCATGGTGGAAGTGAATCAAATTGAATATAGATTGAAAGTGTTCTTTCTAATTTCTGTAAAATCCTTTGGATTTTTGGTTTTGACCTGTCATAATGTCCATGAAGATTTAGGAATTTGTGCTTCTTAAGGGTTGTTGTAAATACTAATACGGTGTCAGAGTTTCTCCCTTGCTAGATTATACTGGATTCTTCCATTAAGTGAATTTTATGGTACCCGATGCAGTACAATCAGTGAGCATCTATGGTTCTCCTCGCCGTTGTGGTGGCCAAGGTGATATACTTTCAGGAAGGCAAGTACTGCCATGAATTAATCTTTTCAACGTACCTTATTTATATTTCTCATTCAAGAGTTGAGAACCCTAAAAGTTCACGACTTTCTTCAACAATGTCTGAAATTGTTTTCTTTCTCCCTGTGGTTTCAGTGTTGCAGTTTTCGTATCTTGGGCACGGCGGCAAGGCTTAGCCACAGATGGGAATTTGGCTACCAGGCAAGTTTTAGAATTTAAACAGCATATGATTTTCAATCTCATCTGACGGATTCTGATTCCATATATGATCTTTTATGAAGCCCTTTTACACATAGCCCAAAGAATCCAACCATATTAGGGTGCATTGCTGGATCTGCTCTACTTAGAAGGGCTGCATCACTTGCATTTGAGAATAAGAAAAGATCAACCCTTACTACCGACATCATCGAGTTCTTGTGGAGAAGGTACGGTGAACGTCGGGGATGGAGATCATTTATATATAAAAAACAGATCAACTTCTAATATTGGTTCCTTCTTGCAGTCTGGAGGATATTTCTCCGGCTCACTGA

mRNA sequence

ATGAGTGGGACTTCTGTTGAGGCTGATGCTGAGAACATTATAAGAGCAATTACACCTTCTCTTGATCCCAACAGATACAAAGGCCAGGCGGGGAAGATAGCTGTTATTGGTGGATGTCGTGAGTACACAGGTGCTCCATACTTCGCTGCTATATCAGCTTTGAAAATTGGTGCAGATTTGTCTCACGTTTTCTGCACAAAAGATGCTGCTCCTGTCATAAAGAGCTATAGTCCAGAGTTAATTGTGCATCCCGTTTTAGAAGAATCATACAGCGTCAGAGATGAGGAAAAGAAGTTCATTGCAGAAAAGGTTCTTGCTGAGGTTGACAAGTGGCTGGAAAGATTTGATTGCCTTGTCATTGGTCCAGGCCTCGGAAGGGACCCATTTCTTCTGGATTGTGTCAGCGAAATCATAAAGCATGCAAGGCAGACCAATATACCAATGGTTATAGACGGGGATGGACTCTTTCTTATCACTGACAATCTTCACCTTGTAAGCAATTACCCCCTGGCCGTCCTAACTCCAAATGTAAACGAATATAAACGTCTTGTGAAAAATGTGCTGCTTGCTGAAATCGATGAACAAGATGCTTCTAAGCAATTGCTGTCCCTCGTCAAAAGGATTGGTGGCCTAACTATACTCAGGAAAGGGAGAGCTGACCTCATCAGTGACGGTGAGACAGTACAATCAGTGAGCATCTATGGTTCTCCTCGCCGTTGTGGTGGCCAAGGTGATATACTTTCAGGAAGTGTTGCAGTTTTCGTATCTTGGGCACGGCGGCAAGGCTTAGCCACAGATGGGAATTTGGCTACCAGCCCTTTTACACATAGCCCAAAGAATCCAACCATATTAGGGTGCATTGCTGGATCTGCTCTACTTAGAAGGGCTGCATCACTTGCATTTGAGAATAAGAAAAGATCAACCCTTACTACCGACATCATCGAGTTCTTGTGGAGAAGTCTGGAGGATATTTCTCCGGCTCACTGA

Coding sequence (CDS)

ATGAGTGGGACTTCTGTTGAGGCTGATGCTGAGAACATTATAAGAGCAATTACACCTTCTCTTGATCCCAACAGATACAAAGGCCAGGCGGGGAAGATAGCTGTTATTGGTGGATGTCGTGAGTACACAGGTGCTCCATACTTCGCTGCTATATCAGCTTTGAAAATTGGTGCAGATTTGTCTCACGTTTTCTGCACAAAAGATGCTGCTCCTGTCATAAAGAGCTATAGTCCAGAGTTAATTGTGCATCCCGTTTTAGAAGAATCATACAGCGTCAGAGATGAGGAAAAGAAGTTCATTGCAGAAAAGGTTCTTGCTGAGGTTGACAAGTGGCTGGAAAGATTTGATTGCCTTGTCATTGGTCCAGGCCTCGGAAGGGACCCATTTCTTCTGGATTGTGTCAGCGAAATCATAAAGCATGCAAGGCAGACCAATATACCAATGGTTATAGACGGGGATGGACTCTTTCTTATCACTGACAATCTTCACCTTGTAAGCAATTACCCCCTGGCCGTCCTAACTCCAAATGTAAACGAATATAAACGTCTTGTGAAAAATGTGCTGCTTGCTGAAATCGATGAACAAGATGCTTCTAAGCAATTGCTGTCCCTCGTCAAAAGGATTGGTGGCCTAACTATACTCAGGAAAGGGAGAGCTGACCTCATCAGTGACGGTGAGACAGTACAATCAGTGAGCATCTATGGTTCTCCTCGCCGTTGTGGTGGCCAAGGTGATATACTTTCAGGAAGTGTTGCAGTTTTCGTATCTTGGGCACGGCGGCAAGGCTTAGCCACAGATGGGAATTTGGCTACCAGCCCTTTTACACATAGCCCAAAGAATCCAACCATATTAGGGTGCATTGCTGGATCTGCTCTACTTAGAAGGGCTGCATCACTTGCATTTGAGAATAAGAAAAGATCAACCCTTACTACCGACATCATCGAGTTCTTGTGGAGAAGTCTGGAGGATATTTCTCCGGCTCACTGA

Protein sequence

MSGTSVEADAENIIRAITPSLDPNRYKGQAGKIAVIGGCREYTGAPYFAAISALKIGADLSHVFCTKDAAPVIKSYSPELIVHPVLEESYSVRDEEKKFIAEKVLAEVDKWLERFDCLVIGPGLGRDPFLLDCVSEIIKHARQTNIPMVIDGDGLFLITDNLHLVSNYPLAVLTPNVNEYKRLVKNVLLAEIDEQDASKQLLSLVKRIGGLTILRKGRADLISDGETVQSVSIYGSPRRCGGQGDILSGSVAVFVSWARRQGLATDGNLATSPFTHSPKNPTILGCIAGSALLRRAASLAFENKKRSTLTTDIIEFLWRSLEDISPAH
Homology
BLAST of Moc05g06050 vs. NCBI nr
Match: XP_022137883.1 (ATP-dependent (S)-NAD(P)H-hydrate dehydratase [Momordica charantia] >XP_022137884.1 ATP-dependent (S)-NAD(P)H-hydrate dehydratase [Momordica charantia] >XP_022137885.1 ATP-dependent (S)-NAD(P)H-hydrate dehydratase [Momordica charantia] >XP_022137886.1 ATP-dependent (S)-NAD(P)H-hydrate dehydratase [Momordica charantia] >XP_022137887.1 ATP-dependent (S)-NAD(P)H-hydrate dehydratase [Momordica charantia])

HSP 1 Score: 649.8 bits (1675), Expect = 1.2e-182
Identity = 328/328 (100.00%), Postives = 328/328 (100.00%), Query Frame = 0

Query: 1   MSGTSVEADAENIIRAITPSLDPNRYKGQAGKIAVIGGCREYTGAPYFAAISALKIGADL 60
           MSGTSVEADAENIIRAITPSLDPNRYKGQAGKIAVIGGCREYTGAPYFAAISALKIGADL
Sbjct: 1   MSGTSVEADAENIIRAITPSLDPNRYKGQAGKIAVIGGCREYTGAPYFAAISALKIGADL 60

Query: 61  SHVFCTKDAAPVIKSYSPELIVHPVLEESYSVRDEEKKFIAEKVLAEVDKWLERFDCLVI 120
           SHVFCTKDAAPVIKSYSPELIVHPVLEESYSVRDEEKKFIAEKVLAEVDKWLERFDCLVI
Sbjct: 61  SHVFCTKDAAPVIKSYSPELIVHPVLEESYSVRDEEKKFIAEKVLAEVDKWLERFDCLVI 120

Query: 121 GPGLGRDPFLLDCVSEIIKHARQTNIPMVIDGDGLFLITDNLHLVSNYPLAVLTPNVNEY 180
           GPGLGRDPFLLDCVSEIIKHARQTNIPMVIDGDGLFLITDNLHLVSNYPLAVLTPNVNEY
Sbjct: 121 GPGLGRDPFLLDCVSEIIKHARQTNIPMVIDGDGLFLITDNLHLVSNYPLAVLTPNVNEY 180

Query: 181 KRLVKNVLLAEIDEQDASKQLLSLVKRIGGLTILRKGRADLISDGETVQSVSIYGSPRRC 240
           KRLVKNVLLAEIDEQDASKQLLSLVKRIGGLTILRKGRADLISDGETVQSVSIYGSPRRC
Sbjct: 181 KRLVKNVLLAEIDEQDASKQLLSLVKRIGGLTILRKGRADLISDGETVQSVSIYGSPRRC 240

Query: 241 GGQGDILSGSVAVFVSWARRQGLATDGNLATSPFTHSPKNPTILGCIAGSALLRRAASLA 300
           GGQGDILSGSVAVFVSWARRQGLATDGNLATSPFTHSPKNPTILGCIAGSALLRRAASLA
Sbjct: 241 GGQGDILSGSVAVFVSWARRQGLATDGNLATSPFTHSPKNPTILGCIAGSALLRRAASLA 300

Query: 301 FENKKRSTLTTDIIEFLWRSLEDISPAH 329
           FENKKRSTLTTDIIEFLWRSLEDISPAH
Sbjct: 301 FENKKRSTLTTDIIEFLWRSLEDISPAH 328

BLAST of Moc05g06050 vs. NCBI nr
Match: XP_023528365.1 (ATP-dependent (S)-NAD(P)H-hydrate dehydratase [Cucurbita pepo subsp. pepo])

HSP 1 Score: 609.0 bits (1569), Expect = 2.4e-170
Identity = 306/328 (93.29%), Postives = 316/328 (96.34%), Query Frame = 0

Query: 1   MSGTSVEADAENIIRAITPSLDPNRYKGQAGKIAVIGGCREYTGAPYFAAISALKIGADL 60
           MSGTS+EADAENI+RAITPSLDP RYKGQAGKIAVIGGCREYTGAPYFAAISALKIGADL
Sbjct: 36  MSGTSMEADAENILRAITPSLDPKRYKGQAGKIAVIGGCREYTGAPYFAAISALKIGADL 95

Query: 61  SHVFCTKDAAPVIKSYSPELIVHPVLEESYSVRDEEKKFIAEKVLAEVDKWLERFDCLVI 120
           SHVFCTKDAAPVIKSYSPELIVHPVLEESYSVRDEEKKF AE+VLAEVDKWLERFDCLVI
Sbjct: 96  SHVFCTKDAAPVIKSYSPELIVHPVLEESYSVRDEEKKFTAERVLAEVDKWLERFDCLVI 155

Query: 121 GPGLGRDPFLLDCVSEIIKHARQTNIPMVIDGDGLFLITDNLHLVSNYPLAVLTPNVNEY 180
           GPGLGRDPFLL+CVSEIIKHARQTNIPM+IDGDGLFLITDNLHLVSNYPLAVLTPNVNEY
Sbjct: 156 GPGLGRDPFLLECVSEIIKHARQTNIPMIIDGDGLFLITDNLHLVSNYPLAVLTPNVNEY 215

Query: 181 KRLVKNVLLAEIDEQDASKQLLSLVKRIGGLTILRKGRADLISDGETVQSVSIYGSPRRC 240
           KRLVKNVLLAE+DEQDA  QLLSL KRIGGLTILRKGRADLI DGETV+SVSIYGSPRRC
Sbjct: 216 KRLVKNVLLAEVDEQDAPNQLLSLTKRIGGLTILRKGRADLICDGETVKSVSIYGSPRRC 275

Query: 241 GGQGDILSGSVAVFVSWARRQGLATDGNLATSPFTHSPKNPTILGCIAGSALLRRAASLA 300
           GGQGDILSGSVAVFVSWAR+QGL TDGNLAT     SPKNPT+LGCIAGSALLR+AASLA
Sbjct: 276 GGQGDILSGSVAVFVSWARQQGLVTDGNLAT-----SPKNPTVLGCIAGSALLRKAASLA 335

Query: 301 FENKKRSTLTTDIIEFLWRSLEDISPAH 329
           FENKKRSTLTTDIIEFLWRSLEDISPAH
Sbjct: 336 FENKKRSTLTTDIIEFLWRSLEDISPAH 358

BLAST of Moc05g06050 vs. NCBI nr
Match: XP_022955699.1 (ATP-dependent (S)-NAD(P)H-hydrate dehydratase [Cucurbita moschata])

HSP 1 Score: 609.0 bits (1569), Expect = 2.4e-170
Identity = 306/328 (93.29%), Postives = 316/328 (96.34%), Query Frame = 0

Query: 1   MSGTSVEADAENIIRAITPSLDPNRYKGQAGKIAVIGGCREYTGAPYFAAISALKIGADL 60
           MSGTS+EADAENI+RAITPSLDP RYKGQAGKIAVIGGCREYTGAPYFAAISALKIGADL
Sbjct: 36  MSGTSMEADAENILRAITPSLDPKRYKGQAGKIAVIGGCREYTGAPYFAAISALKIGADL 95

Query: 61  SHVFCTKDAAPVIKSYSPELIVHPVLEESYSVRDEEKKFIAEKVLAEVDKWLERFDCLVI 120
           SHVFCTKDAAPVIKSYSPELIVHPVLEESYSVRDEEKKF AE+VLAEVDKWLERFDCLVI
Sbjct: 96  SHVFCTKDAAPVIKSYSPELIVHPVLEESYSVRDEEKKFTAERVLAEVDKWLERFDCLVI 155

Query: 121 GPGLGRDPFLLDCVSEIIKHARQTNIPMVIDGDGLFLITDNLHLVSNYPLAVLTPNVNEY 180
           GPGLGRDPFLL+CVSEIIKHARQTNIPM+IDGDGLFLITDNLHLVSNYPLAVLTPNVNEY
Sbjct: 156 GPGLGRDPFLLECVSEIIKHARQTNIPMIIDGDGLFLITDNLHLVSNYPLAVLTPNVNEY 215

Query: 181 KRLVKNVLLAEIDEQDASKQLLSLVKRIGGLTILRKGRADLISDGETVQSVSIYGSPRRC 240
           KRLVKNVLLAE+DEQDA  QLLSL KRIGGLTILRKGRADLI DGETV+SVSIYGSPRRC
Sbjct: 216 KRLVKNVLLAEVDEQDAPNQLLSLAKRIGGLTILRKGRADLICDGETVKSVSIYGSPRRC 275

Query: 241 GGQGDILSGSVAVFVSWARRQGLATDGNLATSPFTHSPKNPTILGCIAGSALLRRAASLA 300
           GGQGDILSGSVAVFVSWAR+QGL TDGNLAT     SPKNPT+LGCIAGSALLR+AASLA
Sbjct: 276 GGQGDILSGSVAVFVSWARQQGLVTDGNLAT-----SPKNPTVLGCIAGSALLRKAASLA 335

Query: 301 FENKKRSTLTTDIIEFLWRSLEDISPAH 329
           FENKKRSTLTTDIIEFLWRSLEDISPAH
Sbjct: 336 FENKKRSTLTTDIIEFLWRSLEDISPAH 358

BLAST of Moc05g06050 vs. NCBI nr
Match: XP_022979515.1 (ATP-dependent (S)-NAD(P)H-hydrate dehydratase [Cucurbita maxima])

HSP 1 Score: 605.1 bits (1559), Expect = 3.5e-169
Identity = 305/328 (92.99%), Postives = 315/328 (96.04%), Query Frame = 0

Query: 1   MSGTSVEADAENIIRAITPSLDPNRYKGQAGKIAVIGGCREYTGAPYFAAISALKIGADL 60
           MSGTS+EADAENI+RAITPSLDP R+KGQAGKIAVIGGCREYTGAPYFAAISALKIGADL
Sbjct: 36  MSGTSMEADAENILRAITPSLDPKRHKGQAGKIAVIGGCREYTGAPYFAAISALKIGADL 95

Query: 61  SHVFCTKDAAPVIKSYSPELIVHPVLEESYSVRDEEKKFIAEKVLAEVDKWLERFDCLVI 120
           SHVFCTKDAAPVIKSYSPELIVHPVLEESYSVRDEEKKF AE+VLAEVDKWLERFDCLVI
Sbjct: 96  SHVFCTKDAAPVIKSYSPELIVHPVLEESYSVRDEEKKFTAERVLAEVDKWLERFDCLVI 155

Query: 121 GPGLGRDPFLLDCVSEIIKHARQTNIPMVIDGDGLFLITDNLHLVSNYPLAVLTPNVNEY 180
           GPGLGRDPFLL+CVSEIIKHARQTNIPMVIDGDGLFLITDNLHLVSNYPLAVLTPNVNEY
Sbjct: 156 GPGLGRDPFLLECVSEIIKHARQTNIPMVIDGDGLFLITDNLHLVSNYPLAVLTPNVNEY 215

Query: 181 KRLVKNVLLAEIDEQDASKQLLSLVKRIGGLTILRKGRADLISDGETVQSVSIYGSPRRC 240
           KRLVKNVLLAE+DEQDA  QLLSL KRIGGLTILRKGRADLI DGETV+SVSIYGSPRRC
Sbjct: 216 KRLVKNVLLAEVDEQDAPNQLLSLAKRIGGLTILRKGRADLICDGETVKSVSIYGSPRRC 275

Query: 241 GGQGDILSGSVAVFVSWARRQGLATDGNLATSPFTHSPKNPTILGCIAGSALLRRAASLA 300
           GGQGDILSGSVAVFVSWAR+QGL  DGNLAT     SPKNPT+LGCIAGSALLR+AASLA
Sbjct: 276 GGQGDILSGSVAVFVSWARQQGLVIDGNLAT-----SPKNPTVLGCIAGSALLRKAASLA 335

Query: 301 FENKKRSTLTTDIIEFLWRSLEDISPAH 329
           FENKKRSTLTTDIIEFLWRSLEDISPAH
Sbjct: 336 FENKKRSTLTTDIIEFLWRSLEDISPAH 358

BLAST of Moc05g06050 vs. NCBI nr
Match: KAG6582278.1 (ATP-dependent (S)-NAD(P)H-hydrate dehydratase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 604.4 bits (1557), Expect = 6.0e-169
Identity = 306/329 (93.01%), Postives = 316/329 (96.05%), Query Frame = 0

Query: 1   MSGTSVEADAENIIRAITPSLDPNRYKGQAGKIAVIGGCREYTGAPYFAAISALKIGADL 60
           MSGTS+EADAENI+RAITPSLDP RYKGQAGKIAVIGGCREYTGAPYFAAISALKIGADL
Sbjct: 36  MSGTSMEADAENILRAITPSLDPKRYKGQAGKIAVIGGCREYTGAPYFAAISALKIGADL 95

Query: 61  SHVFCTKDAAPVIKSYSPELIVHPVLEESYSVRD-EEKKFIAEKVLAEVDKWLERFDCLV 120
           SHVFCTKDAAPVIKSYSPELIVHPVLEESYSVRD EEKKF AE+VLAEVDKWLERFDCLV
Sbjct: 96  SHVFCTKDAAPVIKSYSPELIVHPVLEESYSVRDEEEKKFTAERVLAEVDKWLERFDCLV 155

Query: 121 IGPGLGRDPFLLDCVSEIIKHARQTNIPMVIDGDGLFLITDNLHLVSNYPLAVLTPNVNE 180
           IGPGLGRDPFLL+CVSEIIKHARQTNIPM+IDGDGLFLITDNLHLVSNYPLAVLTPNVNE
Sbjct: 156 IGPGLGRDPFLLECVSEIIKHARQTNIPMIIDGDGLFLITDNLHLVSNYPLAVLTPNVNE 215

Query: 181 YKRLVKNVLLAEIDEQDASKQLLSLVKRIGGLTILRKGRADLISDGETVQSVSIYGSPRR 240
           YKRLVKNVLLAE+DEQDA  QLLSL KRIGGLTILRKGRADLI DGETV+SVSIYGSPRR
Sbjct: 216 YKRLVKNVLLAEVDEQDAPNQLLSLAKRIGGLTILRKGRADLICDGETVKSVSIYGSPRR 275

Query: 241 CGGQGDILSGSVAVFVSWARRQGLATDGNLATSPFTHSPKNPTILGCIAGSALLRRAASL 300
           CGGQGDILSGSVAVFVSWAR+QGL TDGNLAT     SPKNPT+LGCIAGSALLR+AASL
Sbjct: 276 CGGQGDILSGSVAVFVSWARQQGLVTDGNLAT-----SPKNPTVLGCIAGSALLRKAASL 335

Query: 301 AFENKKRSTLTTDIIEFLWRSLEDISPAH 329
           AFENKKRSTLTTDIIEFLWRSLEDISPAH
Sbjct: 336 AFENKKRSTLTTDIIEFLWRSLEDISPAH 359

BLAST of Moc05g06050 vs. ExPASy Swiss-Prot
Match: F6HDM2 (ATP-dependent (S)-NAD(P)H-hydrate dehydratase OS=Vitis vinifera OX=29760 GN=VIT_05s0020g02800 PE=3 SV=1)

HSP 1 Score: 505.0 bits (1299), Expect = 6.5e-142
Identity = 256/323 (79.26%), Postives = 291/323 (90.09%), Query Frame = 0

Query: 5   SVEADAENIIRAITPSLDPNRYKGQAGKIAVIGGCREYTGAPYFAAISALKIGADLSHVF 64
           ++EADAENI+RAITP+LD  R+KGQAGKIAVIGGCREYTGAPYF+AISALKIGADLSHVF
Sbjct: 37  ALEADAENILRAITPTLDLARHKGQAGKIAVIGGCREYTGAPYFSAISALKIGADLSHVF 96

Query: 65  CTKDAAPVIKSYSPELIVHPVLEESYSVRDEEKKFIAEKVLAEVDKWLERFDCLVIGPGL 124
           CTKDAAPVIKSYSPELIVHP+LEESYSVR+E+KK I+EKVL EV KW+ERFDCLV+GPGL
Sbjct: 97  CTKDAAPVIKSYSPELIVHPLLEESYSVREEDKKAISEKVLTEVVKWMERFDCLVVGPGL 156

Query: 125 GRDPFLLDCVSEIIKHARQTNIPMVIDGDGLFLITDNLHLVSNYPLAVLTPNVNEYKRLV 184
           GRDPFLL CVSEI+KHARQ+N+P+VIDGDGLFL+T++L LVS YPLAVLTPNVNEYKRLV
Sbjct: 157 GRDPFLLGCVSEIMKHARQSNVPIVIDGDGLFLVTNSLDLVSGYPLAVLTPNVNEYKRLV 216

Query: 185 KNVLLAEIDEQDASKQLLSLVKRIGGLTILRKGRADLISDGETVQSVSIYGSPRRCGGQG 244
           + VL  E+ +QDA++QLLSL K IGG+TILRKG++DLISDGETV SV IYGSPRRCGGQG
Sbjct: 217 QKVLNCEVGDQDAAEQLLSLAKGIGGVTILRKGKSDLISDGETVNSVGIYGSPRRCGGQG 276

Query: 245 DILSGSVAVFVSWARRQGLATDGNLATSPFTHSPKNPTILGCIAGSALLRRAASLAFENK 304
           DILSGSVAVF+SWA RQ +  +G+L       SPK+PT+LG IAGSAL+R+AASLAFENK
Sbjct: 277 DILSGSVAVFLSWA-RQRIIAEGDL-----NISPKSPTVLGSIAGSALMRKAASLAFENK 336

Query: 305 KRSTLTTDIIEFLWRSLEDISPA 328
           KRSTLT DIIE L RSLEDI PA
Sbjct: 337 KRSTLTGDIIECLGRSLEDICPA 353

BLAST of Moc05g06050 vs. ExPASy Swiss-Prot
Match: Q94AF2 (ATP-dependent (S)-NAD(P)H-hydrate dehydratase OS=Arabidopsis thaliana OX=3702 GN=At5g19150 PE=1 SV=1)

HSP 1 Score: 463.4 bits (1191), Expect = 2.2e-129
Identity = 240/330 (72.73%), Postives = 280/330 (84.85%), Query Frame = 0

Query: 1   MSGTSVEADAENIIRAITPSLDPNRYKGQAGKIAVIGGCREYTGAPYFAAISALKIGADL 60
           MS TS EADAE+++R +TPSLD  R+KGQAGKIAVIGGCREYTGAPYFAAISALKIGADL
Sbjct: 45  MSSTS-EADAESVLRTVTPSLDLKRHKGQAGKIAVIGGCREYTGAPYFAAISALKIGADL 104

Query: 61  SHVFCTKDAAPVIKSYSPELIVHPVLEESYSV---RDEEKKFIAEKVLAEVDKWLERFDC 120
           SHVFCTKDAAPVIKSYSPELIVHPVLEESYS+    +E+K+ + +KVL EV KW+ERFDC
Sbjct: 105 SHVFCTKDAAPVIKSYSPELIVHPVLEESYSISQLSEEDKREVQDKVLGEVGKWMERFDC 164

Query: 121 LVIGPGLGRDPFLLDCVSEIIKHARQTNIPMVIDGDGLFLITDNLHLVSNYPLAVLTPNV 180
           LVIGPGLGRDPFLL+CVS I+  A+++N+P VIDGDGLFL+T+++ LV +YPLAVLTPNV
Sbjct: 165 LVIGPGLGRDPFLLECVSIIMLLAKKSNVPFVIDGDGLFLVTNSIDLVHSYPLAVLTPNV 224

Query: 181 NEYKRLVKNVLLAEIDEQDASKQLLSLVKRIGGLTILRKGRADLISDGETVQSVSIYGSP 240
           NEYKRLV+ VL  E+DEQ+A  QL SL K+IGG+TILRKG++DLIS+GETV+SVSIYGSP
Sbjct: 225 NEYKRLVQKVLNCEVDEQNAEDQLRSLAKQIGGVTILRKGKSDLISNGETVKSVSIYGSP 284

Query: 241 RRCGGQGDILSGSVAVFVSWARRQGLATDGNLATSPFTHSPKNPTILGCIAGSALLRRAA 300
           RRCGGQGDILSG VAVF+SWA++        L + P + S +NP ILGCIA S LLR+AA
Sbjct: 285 RRCGGQGDILSGGVAVFLSWAQQ--------LKSDPESPS-ENPAILGCIAASGLLRKAA 344

Query: 301 SLAFENKKRSTLTTDIIEFLWRSLEDISPA 328
           SLAF   KRSTLT+DIIE L  SLEDI PA
Sbjct: 345 SLAFTKHKRSTLTSDIIECLGESLEDICPA 364

BLAST of Moc05g06050 vs. ExPASy Swiss-Prot
Match: C5Y210 (ATP-dependent (S)-NAD(P)H-hydrate dehydratase OS=Sorghum bicolor OX=4558 GN=Sb05g010020 PE=3 SV=1)

HSP 1 Score: 432.6 bits (1111), Expect = 4.1e-120
Identity = 218/326 (66.87%), Postives = 260/326 (79.75%), Query Frame = 0

Query: 2   SGTSVEADAENIIRAITPSLDPNRYKGQAGKIAVIGGCREYTGAPYFAAISALKIGADLS 61
           +GT  EADAE ++R ITP LD  R+KGQAGKIAVIGGCREYTGAPYFAAISALK+GADLS
Sbjct: 76  AGTVYEADAEAVVRRITPPLDRARHKGQAGKIAVIGGCREYTGAPYFAAISALKVGADLS 135

Query: 62  HVFCTKDAAPVIKSYSPELIVHPVLEESYSVRDEEKKFIAEKVLAEVDKWLERFDCLVIG 121
           HVFCTKDAA VIKSYSPELIVHP+LEESYSVRD+E+  ++ K+L EV KW+ERFDC+V+G
Sbjct: 136 HVFCTKDAATVIKSYSPELIVHPILEESYSVRDDERASVSSKILTEVGKWMERFDCIVVG 195

Query: 122 PGLGRDPFLLDCVSEIIKHARQTNIPMVIDGDGLFLITDNLHLVSNYPLAVLTPNVNEYK 181
           PGLGRD FLLDCVS I++HARQ NIP V+DGDGLFL+T+NL LV   PLA+LTPNV EYK
Sbjct: 196 PGLGRDSFLLDCVSNIMRHARQANIPTVVDGDGLFLVTNNLSLVEGNPLAILTPNVYEYK 255

Query: 182 RLVKNVLLAEIDEQDASKQLLSLVKRIGGLTILRKGRADLISDGETVQSVSIYGSPRRCG 241
           RLV+ VL  ++DE+ AS+QL++L ++IG +TI++KG+AD+ISDG+TV  VS +GSPRRCG
Sbjct: 256 RLVQKVLNCDVDEETASEQLITLCQKIGDVTIMQKGKADVISDGKTVTQVSTFGSPRRCG 315

Query: 242 GQGDILSGSVAVFVSWARRQGLATDGNLATSPFTHSPKNPTILGCIAGSALLRRAASLAF 301
           GQGDILSGSVAVF SWAR              F  + + PT           R+AAS AF
Sbjct: 316 GQGDILSGSVAVFASWARH-------------FVLTNEQPT---------EKRKAASHAF 375

Query: 302 ENKKRSTLTTDIIEFLWRSLEDISPA 328
           E  KRST+T+DIIEFL +SLEDI PA
Sbjct: 376 EKNKRSTVTSDIIEFLGKSLEDICPA 379

BLAST of Moc05g06050 vs. ExPASy Swiss-Prot
Match: A9RY03 (ATP-dependent (S)-NAD(P)H-hydrate dehydratase OS=Physcomitrium patens OX=3218 GN=PHYPADRAFT_121160 PE=3 SV=1)

HSP 1 Score: 365.2 bits (936), Expect = 8.0e-100
Identity = 195/336 (58.04%), Postives = 237/336 (70.54%), Query Frame = 0

Query: 1   MSGTSVEADAENIIRA---ITPSLDPNRYKGQAGKIAVIGGCREYTGAPYFAAISALKIG 60
           M G S E   + I+ A   + P L P R+KGQAGKIAVIGGCREYTGAPYFAAISALK+G
Sbjct: 1   MGGNSTE--LQKILPALEKVVPVLAPGRHKGQAGKIAVIGGCREYTGAPYFAAISALKMG 60

Query: 61  ADLSHVFCTKDAAPVIKSYSPELIVHPVLEESYSV---RDEEKKFIAEKVLAEVDKWLER 120
           ADL+HVFCT  AA VIKSYSPELIVHPVL ESY V    +EE   + +KVLAEV KWL+R
Sbjct: 61  ADLAHVFCTSGAATVIKSYSPELIVHPVLHESYDVGEIGEEEISGLKDKVLAEVGKWLQR 120

Query: 121 FDCLVIGPGLGRDPFLLDCVSEIIKHARQTNIPMVIDGDGLFLITDNLHLVSNYPLAVLT 180
           FDC+VIGPGLGRDP LLDCV+ II+ A+  NIP+V+DGDGLFL+T+   L+  YPLA+LT
Sbjct: 121 FDCIVIGPGLGRDPILLDCVAAIIEEAKFKNIPLVLDGDGLFLVTNQPELIIGYPLAILT 180

Query: 181 PNVNEYKRLVKNVL---------LAEIDEQDASKQLLSLVKRIGGLTILRKGRADLISDG 240
           PNV E+KRLV  ++           E+  +D   QL  L KR+ G+TIL+KG+ D ISDG
Sbjct: 181 PNVMEHKRLVAKIVGERDQNVPQNPEVSNEDLPGQLQDLAKRMEGVTILQKGKTDYISDG 240

Query: 241 ETVQSVSIYGSPRRCGGQGDILSGSVAVFVSWARRQGLATDGNLATSPFTHSPKNPTILG 300
           +TV S   YGSPRRCGGQGD+LSGS AVFVSWA+      +             NPT++G
Sbjct: 241 KTVLSSDYYGSPRRCGGQGDVLSGSTAVFVSWAKEYFSNENAAGKKEVEERVSNNPTMVG 300

Query: 301 CIAGSALLRRAASLAFENKKRSTLTTDIIEFLWRSL 322
            +AGS L R++A+ AF   KR+T TT+IIE+L   L
Sbjct: 301 ALAGSLLCRKSAANAFAQHKRATTTTNIIEYLGHRL 334

BLAST of Moc05g06050 vs. ExPASy Swiss-Prot
Match: E1BNQ4 (ATP-dependent (S)-NAD(P)H-hydrate dehydratase OS=Bos taurus OX=9913 GN=NAXD PE=3 SV=1)

HSP 1 Score: 273.5 bits (698), Expect = 3.2e-72
Identity = 144/302 (47.68%), Postives = 193/302 (63.91%), Query Frame = 0

Query: 13  IIRAITPSLDPNRYKGQAGKIAVIGGCREYTGAPYFAAISALKIGADLSHVFCTKDAAPV 72
           ++R++ P+L   ++KGQ G+I V+GGCREYTGAPYFAAISALK+GADLSHVFCT++AAPV
Sbjct: 38  LVRSVVPALTTKKHKGQDGRIGVVGGCREYTGAPYFAAISALKVGADLSHVFCTQEAAPV 97

Query: 73  IKSYSPELIVHPVLEESYSVRDEEKKFIAEKVLAEVDKWLERFDCLVIGPGLGRDPFLLD 132
           IK+YSPELIVHPVL+   +VRD             V++WL R   LV+GPGLGRD  LL+
Sbjct: 98  IKAYSPELIVHPVLDSPEAVRD-------------VEQWLPRLHALVVGPGLGRDDALLE 157

Query: 133 CVSEIIKHARQTNIPMVIDGDGLFLITDNLHLVSNYPLAVLTPNVNEYKRLVKNVLLAEI 192
            V  I++ ++   IP+VID DGL+LI     L+  Y  AVLTPN  E+ RL + VL   +
Sbjct: 158 NVKGILEASKARGIPVVIDADGLWLIAQQPALIQGYRKAVLTPNHVEFGRLSEAVLGVPL 217

Query: 193 DEQDASKQLLSLVKRIGGLTILRKGRADLISDGETVQSVSIYGSPRRCGGQGDILSGSVA 252
           D  D    +L L + +G +T+++KG  D+ISDGE V   S  GS RRCGGQGD+LSGS+ 
Sbjct: 218 DGGDRHGAVLRLSQALGNVTVVQKGEQDVISDGEQVLECSQEGSGRRCGGQGDLLSGSLG 277

Query: 253 VFVSWARRQGLATDGNLATSPFTHSPKNPTILGCIAGSALLRRAASLAFENKKRSTLTTD 312
           V   WA R G    G            +P ++      AL R+ +  AF+   R+T T+D
Sbjct: 278 VLAHWALRAGPQKTGG----------PSPLLVAAFGACALTRQCSQQAFQKYGRATTTSD 316

Query: 313 II 315
           ++
Sbjct: 338 MV 316

BLAST of Moc05g06050 vs. ExPASy TrEMBL
Match: A0A6J1C8J1 (ATP-dependent (S)-NAD(P)H-hydrate dehydratase OS=Momordica charantia OX=3673 GN=LOC111009202 PE=3 SV=1)

HSP 1 Score: 649.8 bits (1675), Expect = 6.0e-183
Identity = 328/328 (100.00%), Postives = 328/328 (100.00%), Query Frame = 0

Query: 1   MSGTSVEADAENIIRAITPSLDPNRYKGQAGKIAVIGGCREYTGAPYFAAISALKIGADL 60
           MSGTSVEADAENIIRAITPSLDPNRYKGQAGKIAVIGGCREYTGAPYFAAISALKIGADL
Sbjct: 1   MSGTSVEADAENIIRAITPSLDPNRYKGQAGKIAVIGGCREYTGAPYFAAISALKIGADL 60

Query: 61  SHVFCTKDAAPVIKSYSPELIVHPVLEESYSVRDEEKKFIAEKVLAEVDKWLERFDCLVI 120
           SHVFCTKDAAPVIKSYSPELIVHPVLEESYSVRDEEKKFIAEKVLAEVDKWLERFDCLVI
Sbjct: 61  SHVFCTKDAAPVIKSYSPELIVHPVLEESYSVRDEEKKFIAEKVLAEVDKWLERFDCLVI 120

Query: 121 GPGLGRDPFLLDCVSEIIKHARQTNIPMVIDGDGLFLITDNLHLVSNYPLAVLTPNVNEY 180
           GPGLGRDPFLLDCVSEIIKHARQTNIPMVIDGDGLFLITDNLHLVSNYPLAVLTPNVNEY
Sbjct: 121 GPGLGRDPFLLDCVSEIIKHARQTNIPMVIDGDGLFLITDNLHLVSNYPLAVLTPNVNEY 180

Query: 181 KRLVKNVLLAEIDEQDASKQLLSLVKRIGGLTILRKGRADLISDGETVQSVSIYGSPRRC 240
           KRLVKNVLLAEIDEQDASKQLLSLVKRIGGLTILRKGRADLISDGETVQSVSIYGSPRRC
Sbjct: 181 KRLVKNVLLAEIDEQDASKQLLSLVKRIGGLTILRKGRADLISDGETVQSVSIYGSPRRC 240

Query: 241 GGQGDILSGSVAVFVSWARRQGLATDGNLATSPFTHSPKNPTILGCIAGSALLRRAASLA 300
           GGQGDILSGSVAVFVSWARRQGLATDGNLATSPFTHSPKNPTILGCIAGSALLRRAASLA
Sbjct: 241 GGQGDILSGSVAVFVSWARRQGLATDGNLATSPFTHSPKNPTILGCIAGSALLRRAASLA 300

Query: 301 FENKKRSTLTTDIIEFLWRSLEDISPAH 329
           FENKKRSTLTTDIIEFLWRSLEDISPAH
Sbjct: 301 FENKKRSTLTTDIIEFLWRSLEDISPAH 328

BLAST of Moc05g06050 vs. ExPASy TrEMBL
Match: A0A6J1GX13 (ATP-dependent (S)-NAD(P)H-hydrate dehydratase OS=Cucurbita moschata OX=3662 GN=LOC111457620 PE=3 SV=1)

HSP 1 Score: 609.0 bits (1569), Expect = 1.2e-170
Identity = 306/328 (93.29%), Postives = 316/328 (96.34%), Query Frame = 0

Query: 1   MSGTSVEADAENIIRAITPSLDPNRYKGQAGKIAVIGGCREYTGAPYFAAISALKIGADL 60
           MSGTS+EADAENI+RAITPSLDP RYKGQAGKIAVIGGCREYTGAPYFAAISALKIGADL
Sbjct: 36  MSGTSMEADAENILRAITPSLDPKRYKGQAGKIAVIGGCREYTGAPYFAAISALKIGADL 95

Query: 61  SHVFCTKDAAPVIKSYSPELIVHPVLEESYSVRDEEKKFIAEKVLAEVDKWLERFDCLVI 120
           SHVFCTKDAAPVIKSYSPELIVHPVLEESYSVRDEEKKF AE+VLAEVDKWLERFDCLVI
Sbjct: 96  SHVFCTKDAAPVIKSYSPELIVHPVLEESYSVRDEEKKFTAERVLAEVDKWLERFDCLVI 155

Query: 121 GPGLGRDPFLLDCVSEIIKHARQTNIPMVIDGDGLFLITDNLHLVSNYPLAVLTPNVNEY 180
           GPGLGRDPFLL+CVSEIIKHARQTNIPM+IDGDGLFLITDNLHLVSNYPLAVLTPNVNEY
Sbjct: 156 GPGLGRDPFLLECVSEIIKHARQTNIPMIIDGDGLFLITDNLHLVSNYPLAVLTPNVNEY 215

Query: 181 KRLVKNVLLAEIDEQDASKQLLSLVKRIGGLTILRKGRADLISDGETVQSVSIYGSPRRC 240
           KRLVKNVLLAE+DEQDA  QLLSL KRIGGLTILRKGRADLI DGETV+SVSIYGSPRRC
Sbjct: 216 KRLVKNVLLAEVDEQDAPNQLLSLAKRIGGLTILRKGRADLICDGETVKSVSIYGSPRRC 275

Query: 241 GGQGDILSGSVAVFVSWARRQGLATDGNLATSPFTHSPKNPTILGCIAGSALLRRAASLA 300
           GGQGDILSGSVAVFVSWAR+QGL TDGNLAT     SPKNPT+LGCIAGSALLR+AASLA
Sbjct: 276 GGQGDILSGSVAVFVSWARQQGLVTDGNLAT-----SPKNPTVLGCIAGSALLRKAASLA 335

Query: 301 FENKKRSTLTTDIIEFLWRSLEDISPAH 329
           FENKKRSTLTTDIIEFLWRSLEDISPAH
Sbjct: 336 FENKKRSTLTTDIIEFLWRSLEDISPAH 358

BLAST of Moc05g06050 vs. ExPASy TrEMBL
Match: A0A6J1ITH2 (ATP-dependent (S)-NAD(P)H-hydrate dehydratase OS=Cucurbita maxima OX=3661 GN=LOC111479195 PE=3 SV=1)

HSP 1 Score: 605.1 bits (1559), Expect = 1.7e-169
Identity = 305/328 (92.99%), Postives = 315/328 (96.04%), Query Frame = 0

Query: 1   MSGTSVEADAENIIRAITPSLDPNRYKGQAGKIAVIGGCREYTGAPYFAAISALKIGADL 60
           MSGTS+EADAENI+RAITPSLDP R+KGQAGKIAVIGGCREYTGAPYFAAISALKIGADL
Sbjct: 36  MSGTSMEADAENILRAITPSLDPKRHKGQAGKIAVIGGCREYTGAPYFAAISALKIGADL 95

Query: 61  SHVFCTKDAAPVIKSYSPELIVHPVLEESYSVRDEEKKFIAEKVLAEVDKWLERFDCLVI 120
           SHVFCTKDAAPVIKSYSPELIVHPVLEESYSVRDEEKKF AE+VLAEVDKWLERFDCLVI
Sbjct: 96  SHVFCTKDAAPVIKSYSPELIVHPVLEESYSVRDEEKKFTAERVLAEVDKWLERFDCLVI 155

Query: 121 GPGLGRDPFLLDCVSEIIKHARQTNIPMVIDGDGLFLITDNLHLVSNYPLAVLTPNVNEY 180
           GPGLGRDPFLL+CVSEIIKHARQTNIPMVIDGDGLFLITDNLHLVSNYPLAVLTPNVNEY
Sbjct: 156 GPGLGRDPFLLECVSEIIKHARQTNIPMVIDGDGLFLITDNLHLVSNYPLAVLTPNVNEY 215

Query: 181 KRLVKNVLLAEIDEQDASKQLLSLVKRIGGLTILRKGRADLISDGETVQSVSIYGSPRRC 240
           KRLVKNVLLAE+DEQDA  QLLSL KRIGGLTILRKGRADLI DGETV+SVSIYGSPRRC
Sbjct: 216 KRLVKNVLLAEVDEQDAPNQLLSLAKRIGGLTILRKGRADLICDGETVKSVSIYGSPRRC 275

Query: 241 GGQGDILSGSVAVFVSWARRQGLATDGNLATSPFTHSPKNPTILGCIAGSALLRRAASLA 300
           GGQGDILSGSVAVFVSWAR+QGL  DGNLAT     SPKNPT+LGCIAGSALLR+AASLA
Sbjct: 276 GGQGDILSGSVAVFVSWARQQGLVIDGNLAT-----SPKNPTVLGCIAGSALLRKAASLA 335

Query: 301 FENKKRSTLTTDIIEFLWRSLEDISPAH 329
           FENKKRSTLTTDIIEFLWRSLEDISPAH
Sbjct: 336 FENKKRSTLTTDIIEFLWRSLEDISPAH 358

BLAST of Moc05g06050 vs. ExPASy TrEMBL
Match: A0A5D3CYW0 (ATP-dependent (S)-NAD(P)H-hydrate dehydratase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold434G00150 PE=3 SV=1)

HSP 1 Score: 600.1 bits (1546), Expect = 5.4e-168
Identity = 301/327 (92.05%), Postives = 314/327 (96.02%), Query Frame = 0

Query: 1   MSGTSVEADAENIIRAITPSLDPNRYKGQAGKIAVIGGCREYTGAPYFAAISALKIGADL 60
           MSGTS+EADAE+I+RAITP LDPNRYKGQAGKIAVIGGCREYTGAPYFAAISALKIGADL
Sbjct: 36  MSGTSIEADAEHILRAITPCLDPNRYKGQAGKIAVIGGCREYTGAPYFAAISALKIGADL 95

Query: 61  SHVFCTKDAAPVIKSYSPELIVHPVLEESYSVRDEEKKFIAEKVLAEVDKWLERFDCLVI 120
           SHVFCTKDAAPVIKSYSPELIVHPVLEESYSVRDEEKKFI+E+VLAEVDKWLERFDCLVI
Sbjct: 96  SHVFCTKDAAPVIKSYSPELIVHPVLEESYSVRDEEKKFISERVLAEVDKWLERFDCLVI 155

Query: 121 GPGLGRDPFLLDCVSEIIKHARQTNIPMVIDGDGLFLITDNLHLVSNYPLAVLTPNVNEY 180
           GPGLGRDPFLLDCVSEIIKHARQTNIPMVIDGDGLFLIT NLHLVSNYPLAVLTPNVNEY
Sbjct: 156 GPGLGRDPFLLDCVSEIIKHARQTNIPMVIDGDGLFLITGNLHLVSNYPLAVLTPNVNEY 215

Query: 181 KRLVKNVLLAEIDEQDASKQLLSLVKRIGGLTILRKGRADLISDGETVQSVSIYGSPRRC 240
           KRLV+NVLLAE+DEQDA  QLLSL KRIGG+TILRKGRADLISDGETV+SVSIYGSPRRC
Sbjct: 216 KRLVENVLLAEVDEQDAPNQLLSLAKRIGGITILRKGRADLISDGETVKSVSIYGSPRRC 275

Query: 241 GGQGDILSGSVAVFVSWARRQGLATDGNLATSPFTHSPKNPTILGCIAGSALLRRAASLA 300
           GGQGDILSGSVAVF+SWA+RQGL TD N+     T SPKNPT+LGCIAGSALLRRAASLA
Sbjct: 276 GGQGDILSGSVAVFISWAQRQGLVTDDNM-----TSSPKNPTVLGCIAGSALLRRAASLA 335

Query: 301 FENKKRSTLTTDIIEFLWRSLEDISPA 328
           FENKKRSTLTTDIIEFLWRSLEDISPA
Sbjct: 336 FENKKRSTLTTDIIEFLWRSLEDISPA 357

BLAST of Moc05g06050 vs. ExPASy TrEMBL
Match: A0A1S3AX65 (ATP-dependent (S)-NAD(P)H-hydrate dehydratase OS=Cucumis melo OX=3656 GN=LOC103483736 PE=3 SV=1)

HSP 1 Score: 599.7 bits (1545), Expect = 7.1e-168
Identity = 301/327 (92.05%), Postives = 314/327 (96.02%), Query Frame = 0

Query: 1   MSGTSVEADAENIIRAITPSLDPNRYKGQAGKIAVIGGCREYTGAPYFAAISALKIGADL 60
           MSGTS+EADAE+I+RAITP LDPNRYKGQAGKIAVIGGCREYTGAPYFAAISALKIGADL
Sbjct: 36  MSGTSIEADAEHILRAITPCLDPNRYKGQAGKIAVIGGCREYTGAPYFAAISALKIGADL 95

Query: 61  SHVFCTKDAAPVIKSYSPELIVHPVLEESYSVRDEEKKFIAEKVLAEVDKWLERFDCLVI 120
           SHVFCTKDAAPVIKSYSPELIVHPVLEESYSVRDEE+KFI+E+VLAEVDKWLERFDCLVI
Sbjct: 96  SHVFCTKDAAPVIKSYSPELIVHPVLEESYSVRDEERKFISERVLAEVDKWLERFDCLVI 155

Query: 121 GPGLGRDPFLLDCVSEIIKHARQTNIPMVIDGDGLFLITDNLHLVSNYPLAVLTPNVNEY 180
           GPGLGRDPFLLDCVSEIIKHARQTNIPMVIDGDGLFLIT NLHLVSNYPLAVLTPNVNEY
Sbjct: 156 GPGLGRDPFLLDCVSEIIKHARQTNIPMVIDGDGLFLITGNLHLVSNYPLAVLTPNVNEY 215

Query: 181 KRLVKNVLLAEIDEQDASKQLLSLVKRIGGLTILRKGRADLISDGETVQSVSIYGSPRRC 240
           KRLV+NVLLAE+DEQDA  QLLSL KRIGGLTILRKGRADLISDGETV+SVSIYGSPRRC
Sbjct: 216 KRLVENVLLAEVDEQDAPNQLLSLAKRIGGLTILRKGRADLISDGETVKSVSIYGSPRRC 275

Query: 241 GGQGDILSGSVAVFVSWARRQGLATDGNLATSPFTHSPKNPTILGCIAGSALLRRAASLA 300
           GGQGDILSGSVAVF+SWA+RQGL TD N+     T SPKNPT+LGCIAGSALLRRAASLA
Sbjct: 276 GGQGDILSGSVAVFISWAQRQGLVTDDNM-----TSSPKNPTVLGCIAGSALLRRAASLA 335

Query: 301 FENKKRSTLTTDIIEFLWRSLEDISPA 328
           FENKKRSTLTTDIIEFLWRSLEDISPA
Sbjct: 336 FENKKRSTLTTDIIEFLWRSLEDISPA 357

BLAST of Moc05g06050 vs. TAIR 10
Match: AT5G19150.1 (pfkB-like carbohydrate kinase family protein )

HSP 1 Score: 463.4 bits (1191), Expect = 1.5e-130
Identity = 240/330 (72.73%), Postives = 280/330 (84.85%), Query Frame = 0

Query: 1   MSGTSVEADAENIIRAITPSLDPNRYKGQAGKIAVIGGCREYTGAPYFAAISALKIGADL 60
           MS TS EADAE+++R +TPSLD  R+KGQAGKIAVIGGCREYTGAPYFAAISALKIGADL
Sbjct: 45  MSSTS-EADAESVLRTVTPSLDLKRHKGQAGKIAVIGGCREYTGAPYFAAISALKIGADL 104

Query: 61  SHVFCTKDAAPVIKSYSPELIVHPVLEESYSV---RDEEKKFIAEKVLAEVDKWLERFDC 120
           SHVFCTKDAAPVIKSYSPELIVHPVLEESYS+    +E+K+ + +KVL EV KW+ERFDC
Sbjct: 105 SHVFCTKDAAPVIKSYSPELIVHPVLEESYSISQLSEEDKREVQDKVLGEVGKWMERFDC 164

Query: 121 LVIGPGLGRDPFLLDCVSEIIKHARQTNIPMVIDGDGLFLITDNLHLVSNYPLAVLTPNV 180
           LVIGPGLGRDPFLL+CVS I+  A+++N+P VIDGDGLFL+T+++ LV +YPLAVLTPNV
Sbjct: 165 LVIGPGLGRDPFLLECVSIIMLLAKKSNVPFVIDGDGLFLVTNSIDLVHSYPLAVLTPNV 224

Query: 181 NEYKRLVKNVLLAEIDEQDASKQLLSLVKRIGGLTILRKGRADLISDGETVQSVSIYGSP 240
           NEYKRLV+ VL  E+DEQ+A  QL SL K+IGG+TILRKG++DLIS+GETV+SVSIYGSP
Sbjct: 225 NEYKRLVQKVLNCEVDEQNAEDQLRSLAKQIGGVTILRKGKSDLISNGETVKSVSIYGSP 284

Query: 241 RRCGGQGDILSGSVAVFVSWARRQGLATDGNLATSPFTHSPKNPTILGCIAGSALLRRAA 300
           RRCGGQGDILSG VAVF+SWA++        L + P + S +NP ILGCIA S LLR+AA
Sbjct: 285 RRCGGQGDILSGGVAVFLSWAQQ--------LKSDPESPS-ENPAILGCIAASGLLRKAA 344

Query: 301 SLAFENKKRSTLTTDIIEFLWRSLEDISPA 328
           SLAF   KRSTLT+DIIE L  SLEDI PA
Sbjct: 345 SLAFTKHKRSTLTSDIIECLGESLEDICPA 364

BLAST of Moc05g06050 vs. TAIR 10
Match: AT5G19150.2 (pfkB-like carbohydrate kinase family protein )

HSP 1 Score: 463.4 bits (1191), Expect = 1.5e-130
Identity = 240/330 (72.73%), Postives = 280/330 (84.85%), Query Frame = 0

Query: 1   MSGTSVEADAENIIRAITPSLDPNRYKGQAGKIAVIGGCREYTGAPYFAAISALKIGADL 60
           MS TS EADAE+++R +TPSLD  R+KGQAGKIAVIGGCREYTGAPYFAAISALKIGADL
Sbjct: 45  MSSTS-EADAESVLRTVTPSLDLKRHKGQAGKIAVIGGCREYTGAPYFAAISALKIGADL 104

Query: 61  SHVFCTKDAAPVIKSYSPELIVHPVLEESYSV---RDEEKKFIAEKVLAEVDKWLERFDC 120
           SHVFCTKDAAPVIKSYSPELIVHPVLEESYS+    +E+K+ + +KVL EV KW+ERFDC
Sbjct: 105 SHVFCTKDAAPVIKSYSPELIVHPVLEESYSISQLSEEDKREVQDKVLGEVGKWMERFDC 164

Query: 121 LVIGPGLGRDPFLLDCVSEIIKHARQTNIPMVIDGDGLFLITDNLHLVSNYPLAVLTPNV 180
           LVIGPGLGRDPFLL+CVS I+  A+++N+P VIDGDGLFL+T+++ LV +YPLAVLTPNV
Sbjct: 165 LVIGPGLGRDPFLLECVSIIMLLAKKSNVPFVIDGDGLFLVTNSIDLVHSYPLAVLTPNV 224

Query: 181 NEYKRLVKNVLLAEIDEQDASKQLLSLVKRIGGLTILRKGRADLISDGETVQSVSIYGSP 240
           NEYKRLV+ VL  E+DEQ+A  QL SL K+IGG+TILRKG++DLIS+GETV+SVSIYGSP
Sbjct: 225 NEYKRLVQKVLNCEVDEQNAEDQLRSLAKQIGGVTILRKGKSDLISNGETVKSVSIYGSP 284

Query: 241 RRCGGQGDILSGSVAVFVSWARRQGLATDGNLATSPFTHSPKNPTILGCIAGSALLRRAA 300
           RRCGGQGDILSG VAVF+SWA++        L + P + S +NP ILGCIA S LLR+AA
Sbjct: 285 RRCGGQGDILSGGVAVFLSWAQQ--------LKSDPESPS-ENPAILGCIAASGLLRKAA 344

Query: 301 SLAFENKKRSTLTTDIIEFLWRSLEDISPA 328
           SLAF   KRSTLT+DIIE L  SLEDI PA
Sbjct: 345 SLAFTKHKRSTLTSDIIECLGESLEDICPA 364

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022137883.11.2e-182100.00ATP-dependent (S)-NAD(P)H-hydrate dehydratase [Momordica charantia] >XP_02213788... [more]
XP_023528365.12.4e-17093.29ATP-dependent (S)-NAD(P)H-hydrate dehydratase [Cucurbita pepo subsp. pepo][more]
XP_022955699.12.4e-17093.29ATP-dependent (S)-NAD(P)H-hydrate dehydratase [Cucurbita moschata][more]
XP_022979515.13.5e-16992.99ATP-dependent (S)-NAD(P)H-hydrate dehydratase [Cucurbita maxima][more]
KAG6582278.16.0e-16993.01ATP-dependent (S)-NAD(P)H-hydrate dehydratase, partial [Cucurbita argyrosperma s... [more]
Match NameE-valueIdentityDescription
F6HDM26.5e-14279.26ATP-dependent (S)-NAD(P)H-hydrate dehydratase OS=Vitis vinifera OX=29760 GN=VIT_... [more]
Q94AF22.2e-12972.73ATP-dependent (S)-NAD(P)H-hydrate dehydratase OS=Arabidopsis thaliana OX=3702 GN... [more]
C5Y2104.1e-12066.87ATP-dependent (S)-NAD(P)H-hydrate dehydratase OS=Sorghum bicolor OX=4558 GN=Sb05... [more]
A9RY038.0e-10058.04ATP-dependent (S)-NAD(P)H-hydrate dehydratase OS=Physcomitrium patens OX=3218 GN... [more]
E1BNQ43.2e-7247.68ATP-dependent (S)-NAD(P)H-hydrate dehydratase OS=Bos taurus OX=9913 GN=NAXD PE=3... [more]
Match NameE-valueIdentityDescription
A0A6J1C8J16.0e-183100.00ATP-dependent (S)-NAD(P)H-hydrate dehydratase OS=Momordica charantia OX=3673 GN=... [more]
A0A6J1GX131.2e-17093.29ATP-dependent (S)-NAD(P)H-hydrate dehydratase OS=Cucurbita moschata OX=3662 GN=L... [more]
A0A6J1ITH21.7e-16992.99ATP-dependent (S)-NAD(P)H-hydrate dehydratase OS=Cucurbita maxima OX=3661 GN=LOC... [more]
A0A5D3CYW05.4e-16892.05ATP-dependent (S)-NAD(P)H-hydrate dehydratase OS=Cucumis melo var. makuwa OX=119... [more]
A0A1S3AX657.1e-16892.05ATP-dependent (S)-NAD(P)H-hydrate dehydratase OS=Cucumis melo OX=3656 GN=LOC1034... [more]
Match NameE-valueIdentityDescription
AT5G19150.11.5e-13072.73pfkB-like carbohydrate kinase family protein [more]
AT5G19150.21.5e-13072.73pfkB-like carbohydrate kinase family protein [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (OHB3-1) v2
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000631ATP-dependent (S)-NAD(P)H-hydrate dehydrataseTIGRFAMTIGR00196TIGR00196coord: 16..257
e-value: 6.8E-49
score: 164.6
IPR000631ATP-dependent (S)-NAD(P)H-hydrate dehydratasePFAMPF01256Carb_kinasecoord: 34..316
e-value: 1.1E-49
score: 169.2
IPR000631ATP-dependent (S)-NAD(P)H-hydrate dehydrataseHAMAPMF_01965NADHX_dehydratasecoord: 10..326
score: 29.143545
IPR000631ATP-dependent (S)-NAD(P)H-hydrate dehydratasePROSITEPS51383YJEF_C_3coord: 10..324
score: 77.930817
IPR000631ATP-dependent (S)-NAD(P)H-hydrate dehydrataseCDDcd01171YXKO-relatedcoord: 23..315
e-value: 4.23884E-83
score: 250.222
IPR029056Ribokinase-likeGENE3D3.40.1190.20coord: 9..324
e-value: 5.7E-80
score: 270.6
IPR029056Ribokinase-likeSUPERFAMILY53613Ribokinase-likecoord: 12..322
NoneNo IPR availablePANTHERPTHR12592ATP-DEPENDENT (S)-NAD(P)H-HYDRATE DEHYDRATASE FAMILY MEMBERcoord: 5..326
NoneNo IPR availablePANTHERPTHR12592:SF1ATP-DEPENDENT (S)-NAD(P)H-HYDRATE DEHYDRATASEcoord: 5..326

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Moc05g06050.1Moc05g06050.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0110051 metabolite repair
biological_process GO:0046496 nicotinamide nucleotide metabolic process
molecular_function GO:0052855 ADP-dependent NAD(P)H-hydrate dehydratase activity
molecular_function GO:0005524 ATP binding
molecular_function GO:0047453 ATP-dependent NAD(P)H-hydrate dehydratase activity