Moc05g06000 (gene) Bitter gourd (OHB3-1) v2

Overview


Name	Moc05g06000
Type	gene
Organism	Momordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
Description	3-dehydrosphinganine reductase TSC10A-like
Location	chr5: 4155799 .. 4159577 (-)
RNA-Seq Expression	Moc05g06000
Synteny	Moc05g06000

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGATCAGTCCGAAAGTGTTGGAGAGAAGAGAAAGCAGTAAAAACCTCTCTGATTCGGTCACCGAGCAGTCGGACGACTCTTCAGTAACCCAAGATGACCCCTTCGACGCTTATTTCGCTGTATACCCCCTCCATCCTTCGGAGGTGGAAGAAAGGTATATATATAGTTTAATCATAGTAGGGCCCCAGAACGCTAAAAAGGTGGGGGAACAAGAGTTGTCACGATAGGAGAGAGAAATGACTAAAAGAAACCAACGATTCTCTCTTCTTAAACAACCTATATCCTCCACACTTAATTAGCATTTGATAGATTATCCAACCCCGAGCAATCTTAGTTATTGGTAGGGGTTCGGTCCGTTAGCAGGTCTTTGTTTAGTCATTCAGATAGTTACTGGCGTTTTTTTAGCTATGACATAGCTCAGCGGTAATTGACATATACCTCCATCTCTCTCTATTTTCCTTGATAAGTGAATGGATCTATAATTTGGAATGAAAAAAAAACTAACGATATTGTTTGATTTTTTCCATAAGTTAAAAAGCGATTTATGTAATTTTAGTACCTGTCGTTTAAAGTTTGTAATGATTTAATCAGTGAATTTGTTTATTAAATTTTTTTTTATATATGTATATTGATAAATCAACAAATGATGTTTATAAATTACAAATACTAAATCGTTATATAATATAAATTGACACTTGATTTTGATAAAATACATTAGGACTCGATTACTACAAATTTAAAACTATAGAGACCAGAATTTGAAAGTACATAGACTCATTTTGGCCAACCCACCATCAAAGTTTTTACTTAGGAGGTGCTCTTGAATTACTGAATATCGTGAACTAAAAAACAATGTTGACGTCTCTAGTAAACCAATGATATTGTTTATTAGGGATTCCTCCTTATTGCATCGTATTCTCTCATATTCTGGTTGTGCCATTCGTGAAAATAAGTTGAAGAGAAGAACCAATTTTCTTGCGGTTTTTTACATTCTCAATCGCCATCATCACCCTAGGTAGAAATTTTCGACCACCCCCAAACCGATGGCTATCGGTTTTTTTTTTCCTTAATTTCTTCGAGCCGTGGAATTGTTATAATCTATCAGAATTTAATCACGCCGTGTTTTATAGGAAAGAAAGGTGTAGGTCTTATTTTGACAAAAATACATGGACCAAGTAACCATACAAAAGCATCATTGTTTTTTAACATTTTTCACATGATATAGATGGACCGCTTAGATCTTGCCACGTGGATAATTTGGGTGACAAAACCCACGAAAGAAACCCAACTTCAAGAAAATCATTTACTTGACTTCTACACTGCAAAGCTTTCGTCAATGGCGGAATTCAATTACGCCTTCATCTCTCTCGTTCTTCTTCTTCTTCCTTTCGCACTCCTCGTCTTCCTCTACTTCATAGTGCGTCCGCGTCCTGTTAAGATTCCTTTGAAGAATCGTCATGTCTTCATTACGGGTGGATCCAGCGGAATCGGGCTCGCGTTGGCTCACCAGGCCGCATCGGAGGGTGCGCGTGTCTCCATCCTTGCGCGCTCTCTAAACAAGCTCGAGGACGCCAAGGACGCAATCCGGCTCTCCACCGGCATCGATGTGGCCGTCTACGCCGCCGATGTCAGAGATTACGATGCCATTTCGAGGGCTGCAGATGAAGCGGGGCCGATTGACGTTCTTATCGTGAATCAAGGCGTGTTCGTACCTCAGGAACTGGCGGAGCAGGGAATGGATGAGGTGAAATTCATGTTAGATGTTAATTTAGTGGGCACTTTTAACATGATTAAGGCTGCCCTACCTGCGATGAAGAATAGAGTGGACCGTCGACCAGCATCCATTGCTCTCATGTCTTCTCAGGCCGGTCAGGTAGTTGCGCTTCTTGTTTCTTTCTACTTTTATTGCCAAATTGTATTGATTGCTGTTTCGTGTACACAAAGGATTCAATGAACGAAGAGAGGAGCATGAAATGTTTGGTATGGTTGAGTGAATAAGTCTTAAAAGTACTTTTTAAAATTTTCAAAATTTTCATAGTTTGGTTACATAATCAATTAAAGAAGCACTTGATGAACGCTCTATGATAAGTGTTTCACCTGAAAACGCTACTCCAGAAAGTCCCAAACTCATTCTTAGTAAAAATTTCTTTAATTATGTTGGAATGGTGAATTTCATGACATCGATGACATTCAAGCACTCACAAGCTGTAGATTCAGTAGAGATGCTAAGTTTTTTACTCCGAAAAATCCTCTTGTAAATCTGTGCAGGTTGGCATTTATGGTTACACGGCTTATTCAGCCAGTAAGTTTGGGCTTCGTGGTTTGGCAGAAGCCTTGCAGCAGGAGGTCATTGGAGATGACATACATATTTCTGTCATATTCCCTCCAGACACTGACACTCCTGGATTAGCAGAAGGTACATATAAAGATAGACCTGTCTTGCTTTATTGTATTGTTCTCATAGTCCTCACTCCTCAGTCAGGGTTTCAATATTTCCCAGGTGGGCTGGAATATTTTTAGCTCAGTTTCCAGTTCAATGGTTTGTATTAAAATATGAGGGGAATGGTTTGGAAGGATAACCAATTAGAGTATTAGCTATGTTTTATTTAAATTAGTAACCATCTATTTTTTGGTTCTTTTTTGGATATCTCAGGCTACATGCTACTTAGAAGGTTTCATTAACAGAAATCCTTAAATTTATAGTATCTTAGTTGGTTTCTCCATTTAAAAGTTACCTACACTTAATTGCAGAACATAAGAGAAGACCTGAATTGACCAGTATCATTGCTGCTGGAGGAGGCAGTATGAAAGCTGAGGCAGTTGCCAAAAAAACTTTAAATGGCATTAAATCTGGTAGTTTTATAGTCCCCTGCAACTTTGAGGGAGCATTATTGTCCATATCAACTGCCGGCTTATCACCTCAGAATTCATTCTGGATGGCATTCCTTGAAGTTGTTTTTGCTGGAATAATAAGGATCGTGGCCTTGTGTTTTCAATGGGTTTGGTATGGAAGTATCGAAAAGTGGAATGCACAAAAGAAAAGTAAGTATTCATTTAAGCTATAAAACTATCAGCTTGTTGAGTCCTTAATTAATATATACTACTAAAATTGCATCAGTTGTCTAGAAGCTCAATGTTTGATTGACTATTAAAGATGCCCTCTTTTTCTGGTTAATAAGATGTATTATTGTTTATTGCACTCATCATGCAGCAATGAGATATGGTTGAAATGCAGTCAAACTTTCATAAACATCGAAGGCTAATTAGATTTTGAGTTAAATACTTGTGCAATATCTGGTTCTGGGTTTTATTTTTGTTTCAAGACAATAATCATCTCTCTTCGAATTTCTAACATACGATGATGCAAATGAATTTGTTTTCCTTCTGGACTTTTTGCTAAGCAAAGCTGAGCTGCCTTTCTGCGCTCACTCGATTGTGAAAGTCACATGAGTTCAGGATTCTAATTACCATTCGTTTCGCTATAAATTCTCATACTCCTGATGTCTGCTATAAATTAGAATCAGTGCTTATGATCTTACTTGTGCCTGAAGCTGATCTATGCTGTGAATTAACCAATTATCTAGAACAACCTGCATTCAACCCGAGAACTTTTGTTACGCTATAGTGAGGTCTATGTGGTTGAGCCCTAATGCTCATATCTGGATAAAATTGGAACTCACTTTTTCTTATAGTTTTCATTTATTTGAGCATTTCTCTCTCTGCCATTTGATGAGCATGTGTTGATAGCCTTATCTATATCTTATTGCCAGGAACGTAG

mRNA sequence

ATGATCAGTCCGAAAGTGTTGGAGAGAAGAGAAAGCAGTAAAAACCTCTCTGATTCGGTCACCGAGCAGTCGGACGACTCTTCAGTAACCCAAGATGACCCCTTCGACGCTTATTTCGCTGTATACCCCCTCCATCCTTCGGAGGTGGAAGAAAGGGATTCCTCCTTATTGCATCGTATTCTCTCATATTCTGGTTGTGCCATTCGTGAAAATAAGTTGAAGAGAAGAACCAATTTTCTTGCGGTTTTTTACATTCTCAATCGCCATCATCACCCTAGCGGAATCGGGCTCGCGTTGGCTCACCAGGCCGCATCGGAGGGTGCGCGTGTCTCCATCCTTGCGCGCTCTCTAAACAAGCTCGAGGACGCCAAGGACGCAATCCGGCTCTCCACCGGCATCGATGTGGCCGTCTACGCCGCCGATGTCAGAGATTACGATGCCATTTCGAGGGCTGCAGATGAAGCGGGGCCGATTGACGTTCTTATCGTGAATCAAGGCGTGTTCGTACCTCAGGAACTGGCGGAGCAGGGAATGGATGAGGTGAAATTCATGTTAGATGTTAATTTAGTGGGCACTTTTAACATGATTAAGGCTGCCCTACCTGCGATGAAGAATAGAGTGGACCGTCGACCAGCATCCATTGCTCTCATGTCTTCTCAGGCCGGTCAGGTTGGCATTTATGGTTACACGGCTTATTCAGCCAGTAAGTTTGGGCTTCGTGGTTTGGCAGAAGCCTTGCAGCAGGAGGTCATTGGAGATGACATACATATTTCTGTCATATTCCCTCCAGACACTGACACTCCTGGATTAGCAGAAGAACATAAGAGAAGACCTGAATTGACCAGTATCATTGCTGCTGGAGGAGGCAGTATGAAAGCTGAGGCAGTTGCCAAAAAAACTTTAAATGGCATTAAATCTGGTAGTTTTATAGTCCCCTGCAACTTTGAGGGAGCATTATTGTCCATATCAACTGCCGGCTTATCACCTCAGAATTCATTCTGGATGGCATTCCTTGAAGTTGTTTTTGCTGGAATAATAAGGATCGTGGCCTTGTGTTTTCAATGGGTTTGGTATGGAAGTATCGAAAAGTGGAATGCACAAAAGAAAAGAACGTAG

Coding sequence (CDS)

ATGATCAGTCCGAAAGTGTTGGAGAGAAGAGAAAGCAGTAAAAACCTCTCTGATTCGGTCACCGAGCAGTCGGACGACTCTTCAGTAACCCAAGATGACCCCTTCGACGCTTATTTCGCTGTATACCCCCTCCATCCTTCGGAGGTGGAAGAAAGGGATTCCTCCTTATTGCATCGTATTCTCTCATATTCTGGTTGTGCCATTCGTGAAAATAAGTTGAAGAGAAGAACCAATTTTCTTGCGGTTTTTTACATTCTCAATCGCCATCATCACCCTAGCGGAATCGGGCTCGCGTTGGCTCACCAGGCCGCATCGGAGGGTGCGCGTGTCTCCATCCTTGCGCGCTCTCTAAACAAGCTCGAGGACGCCAAGGACGCAATCCGGCTCTCCACCGGCATCGATGTGGCCGTCTACGCCGCCGATGTCAGAGATTACGATGCCATTTCGAGGGCTGCAGATGAAGCGGGGCCGATTGACGTTCTTATCGTGAATCAAGGCGTGTTCGTACCTCAGGAACTGGCGGAGCAGGGAATGGATGAGGTGAAATTCATGTTAGATGTTAATTTAGTGGGCACTTTTAACATGATTAAGGCTGCCCTACCTGCGATGAAGAATAGAGTGGACCGTCGACCAGCATCCATTGCTCTCATGTCTTCTCAGGCCGGTCAGGTTGGCATTTATGGTTACACGGCTTATTCAGCCAGTAAGTTTGGGCTTCGTGGTTTGGCAGAAGCCTTGCAGCAGGAGGTCATTGGAGATGACATACATATTTCTGTCATATTCCCTCCAGACACTGACACTCCTGGATTAGCAGAAGAACATAAGAGAAGACCTGAATTGACCAGTATCATTGCTGCTGGAGGAGGCAGTATGAAAGCTGAGGCAGTTGCCAAAAAAACTTTAAATGGCATTAAATCTGGTAGTTTTATAGTCCCCTGCAACTTTGAGGGAGCATTATTGTCCATATCAACTGCCGGCTTATCACCTCAGAATTCATTCTGGATGGCATTCCTTGAAGTTGTTTTTGCTGGAATAATAAGGATCGTGGCCTTGTGTTTTCAATGGGTTTGGTATGGAAGTATCGAAAAGTGGAATGCACAAAAGAAAAGAACGTAG

Protein sequence

MISPKVLERRESSKNLSDSVTEQSDDSSVTQDDPFDAYFAVYPLHPSEVEERDSSLLHRILSYSGCAIRENKLKRRTNFLAVFYILNRHHHPSGIGLALAHQAASEGARVSILARSLNKLEDAKDAIRLSTGIDVAVYAADVRDYDAISRAADEAGPIDVLIVNQGVFVPQELAEQGMDEVKFMLDVNLVGTFNMIKAALPAMKNRVDRRPASIALMSSQAGQVGIYGYTAYSASKFGLRGLAEALQQEVIGDDIHISVIFPPDTDTPGLAEEHKRRPELTSIIAAGGGSMKAEAVAKKTLNGIKSGSFIVPCNFEGALLSISTAGLSPQNSFWMAFLEVVFAGIIRIVALCFQWVWYGSIEKWNAQKKRT

Homology

[Close]

BLAST of Moc05g06000 vs. NCBI nr
Match: XP_022138240.1 (3-dehydrosphinganine reductase TSC10A-like [Momordica charantia])

HSP 1 Score: 540.8 bits (1392), Expect = 9.2e-150
Identity = 279/279 (100.00%), Postives = 279/279 (100.00%), Query Frame = 0


Query: 93  SGIGLALAHQAASEGARVSILARSLNKLEDAKDAIRLSTGIDVAVYAADVRDYDAISRAA 152
           SGIGLALAHQAASEGARVSILARSLNKLEDAKDAIRLSTGIDVAVYAADVRDYDAISRAA
Sbjct: 49  SGIGLALAHQAASEGARVSILARSLNKLEDAKDAIRLSTGIDVAVYAADVRDYDAISRAA 108

Query: 153 DEAGPIDVLIVNQGVFVPQELAEQGMDEVKFMLDVNLVGTFNMIKAALPAMKNRVDRRPA 212
           DEAGPIDVLIVNQGVFVPQELAEQGMDEVKFMLDVNLVGTFNMIKAALPAMKNRVDRRPA
Sbjct: 109 DEAGPIDVLIVNQGVFVPQELAEQGMDEVKFMLDVNLVGTFNMIKAALPAMKNRVDRRPA 168

Query: 213 SIALMSSQAGQVGIYGYTAYSASKFGLRGLAEALQQEVIGDDIHISVIFPPDTDTPGLAE 272
           SIALMSSQAGQVGIYGYTAYSASKFGLRGLAEALQQEVIGDDIHISVIFPPDTDTPGLAE
Sbjct: 169 SIALMSSQAGQVGIYGYTAYSASKFGLRGLAEALQQEVIGDDIHISVIFPPDTDTPGLAE 228

Query: 273 EHKRRPELTSIIAAGGGSMKAEAVAKKTLNGIKSGSFIVPCNFEGALLSISTAGLSPQNS 332
           EHKRRPELTSIIAAGGGSMKAEAVAKKTLNGIKSGSFIVPCNFEGALLSISTAGLSPQNS
Sbjct: 229 EHKRRPELTSIIAAGGGSMKAEAVAKKTLNGIKSGSFIVPCNFEGALLSISTAGLSPQNS 288

Query: 333 FWMAFLEVVFAGIIRIVALCFQWVWYGSIEKWNAQKKRT 372
           FWMAFLEVVFAGIIRIVALCFQWVWYGSIEKWNAQKKRT
Sbjct: 289 FWMAFLEVVFAGIIRIVALCFQWVWYGSIEKWNAQKKRT 327

[Close]

BLAST of Moc05g06000 vs. NCBI nr
Match: XP_016899126.1 (PREDICTED: 3-dehydrosphinganine reductase TSC10A isoform X1 [Cucumis melo])

HSP 1 Score: 492.7 bits (1267), Expect = 2.9e-135
Identity = 250/277 (90.25%), Postives = 266/277 (96.03%), Query Frame = 0


Query: 93  SGIGLALAHQAASEGARVSILARSLNKLEDAKDAIRLSTGIDVAVYAADVRDYDAISRAA 152
           SGIGLALAHQAA+EGARVSILARSL KLE+AKDAIRLSTGIDVAVYAADVRDYDAIS+A 
Sbjct: 48  SGIGLALAHQAATEGARVSILARSLKKLEEAKDAIRLSTGIDVAVYAADVRDYDAISKAV 107

Query: 153 DEAGPIDVLIVNQGVFVPQELAEQGMDEVKFMLDVNLVGTFNMIKAALPAMKNRVDRRPA 212
           DEAGPIDVLIVNQGVFVPQELAEQ +DEVKFMLDVNL+GTFNMIKAALPAMKNRVDRRPA
Sbjct: 108 DEAGPIDVLIVNQGVFVPQELAEQELDEVKFMLDVNLLGTFNMIKAALPAMKNRVDRRPA 167

Query: 213 SIALMSSQAGQVGIYGYTAYSASKFGLRGLAEALQQEVIGDDIHISVIFPPDTDTPGLAE 272
           SIALMSSQAGQVGIYGYTAYSASKFG+RGLAEALQQEVIGDDIHIS+IFPPDTDTPG AE
Sbjct: 168 SIALMSSQAGQVGIYGYTAYSASKFGIRGLAEALQQEVIGDDIHISLIFPPDTDTPGFAE 227

Query: 273 EHKRRPELTSIIAAGGGSMKAEAVAKKTLNGIKSGSFIVPCNFEGALLSISTAGLSPQNS 332
           E K+RPELT+IIAAGGGSMK E VAKK  NGIKSGSFI+PCNFEGALLS++TAGLSPQ S
Sbjct: 228 EQKKRPELTTIIAAGGGSMKPELVAKKAFNGIKSGSFIIPCNFEGALLSVATAGLSPQRS 287

Query: 333 FWMAFLEVVFAGIIRIVALCFQWVWYGSIEKWNAQKK 370
           F+MAFLEVV AG+IRIVALCFQWVWYGSIEKW+AQ+K
Sbjct: 288 FFMAFLEVVGAGVIRIVALCFQWVWYGSIEKWHAQRK 324

[Close]

BLAST of Moc05g06000 vs. NCBI nr
Match: XP_008438707.1 (PREDICTED: 3-dehydrosphinganine reductase TSC10A isoform X2 [Cucumis melo] >KAA0049383.1 3-dehydrosphinganine reductase TSC10A isoform X2 [Cucumis melo var. makuwa] >TYK17176.1 3-dehydrosphinganine reductase TSC10A isoform X2 [Cucumis melo var. makuwa])

HSP 1 Score: 492.7 bits (1267), Expect = 2.9e-135
Identity = 250/277 (90.25%), Postives = 266/277 (96.03%), Query Frame = 0


Query: 93  SGIGLALAHQAASEGARVSILARSLNKLEDAKDAIRLSTGIDVAVYAADVRDYDAISRAA 152
           SGIGLALAHQAA+EGARVSILARSL KLE+AKDAIRLSTGIDVAVYAADVRDYDAIS+A 
Sbjct: 48  SGIGLALAHQAATEGARVSILARSLKKLEEAKDAIRLSTGIDVAVYAADVRDYDAISKAV 107

Query: 153 DEAGPIDVLIVNQGVFVPQELAEQGMDEVKFMLDVNLVGTFNMIKAALPAMKNRVDRRPA 212
           DEAGPIDVLIVNQGVFVPQELAEQ +DEVKFMLDVNL+GTFNMIKAALPAMKNRVDRRPA
Sbjct: 108 DEAGPIDVLIVNQGVFVPQELAEQELDEVKFMLDVNLLGTFNMIKAALPAMKNRVDRRPA 167

Query: 213 SIALMSSQAGQVGIYGYTAYSASKFGLRGLAEALQQEVIGDDIHISVIFPPDTDTPGLAE 272
           SIALMSSQAGQVGIYGYTAYSASKFG+RGLAEALQQEVIGDDIHIS+IFPPDTDTPG AE
Sbjct: 168 SIALMSSQAGQVGIYGYTAYSASKFGIRGLAEALQQEVIGDDIHISLIFPPDTDTPGFAE 227

Query: 273 EHKRRPELTSIIAAGGGSMKAEAVAKKTLNGIKSGSFIVPCNFEGALLSISTAGLSPQNS 332
           E K+RPELT+IIAAGGGSMK E VAKK  NGIKSGSFI+PCNFEGALLS++TAGLSPQ S
Sbjct: 228 EQKKRPELTTIIAAGGGSMKPELVAKKAFNGIKSGSFIIPCNFEGALLSVATAGLSPQRS 287

Query: 333 FWMAFLEVVFAGIIRIVALCFQWVWYGSIEKWNAQKK 370
           F+MAFLEVV AG+IRIVALCFQWVWYGSIEKW+AQ+K
Sbjct: 288 FFMAFLEVVGAGVIRIVALCFQWVWYGSIEKWHAQRK 324

[Close]

BLAST of Moc05g06000 vs. NCBI nr
Match: XP_004134147.1 (3-dehydrosphinganine reductase TSC10A [Cucumis sativus] >KGN57010.1 hypothetical protein Csa_009962 [Cucumis sativus])

HSP 1 Score: 490.0 bits (1260), Expect = 1.9e-134
Identity = 248/279 (88.89%), Postives = 265/279 (94.98%), Query Frame = 0


Query: 93  SGIGLALAHQAASEGARVSILARSLNKLEDAKDAIRLSTGIDVAVYAADVRDYDAISRAA 152
           SGIGLALAHQAA+EGARVSILARSL KLE+AKDAIRLSTGIDVAVYAADVRDYDAI +A 
Sbjct: 48  SGIGLALAHQAATEGARVSILARSLKKLEEAKDAIRLSTGIDVAVYAADVRDYDAILKAV 107

Query: 153 DEAGPIDVLIVNQGVFVPQELAEQGMDEVKFMLDVNLVGTFNMIKAALPAMKNRVDRRPA 212
           DEAGPIDVLIVNQGVFVPQELAEQ +DEVKFMLDVNL+GTFNMIKAALPAMKNRVDRRPA
Sbjct: 108 DEAGPIDVLIVNQGVFVPQELAEQELDEVKFMLDVNLLGTFNMIKAALPAMKNRVDRRPA 167

Query: 213 SIALMSSQAGQVGIYGYTAYSASKFGLRGLAEALQQEVIGDDIHISVIFPPDTDTPGLAE 272
           SIALMSSQAGQVGIYGYTAYSASKFG+RGLAEALQQEVIGDDIH+S+IFPPDTDTPG AE
Sbjct: 168 SIALMSSQAGQVGIYGYTAYSASKFGIRGLAEALQQEVIGDDIHVSLIFPPDTDTPGFAE 227

Query: 273 EHKRRPELTSIIAAGGGSMKAEAVAKKTLNGIKSGSFIVPCNFEGALLSISTAGLSPQNS 332
           E K+RPELT+IIAAGGGSMK E VAKK  NGIKSGSFI+PCNFEG LLSI+TAGLSPQ S
Sbjct: 228 EQKKRPELTTIIAAGGGSMKPELVAKKAFNGIKSGSFIIPCNFEGTLLSIATAGLSPQRS 287

Query: 333 FWMAFLEVVFAGIIRIVALCFQWVWYGSIEKWNAQKKRT 372
           F+MAF+EVV AG+IRIVALCFQWVWYGSIEKW+AQ+ RT
Sbjct: 288 FFMAFIEVVGAGVIRIVALCFQWVWYGSIEKWHAQRNRT 326

[Close]

BLAST of Moc05g06000 vs. NCBI nr
Match: XP_023539235.1 (3-dehydrosphinganine reductase TSC10A-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 488.4 bits (1256), Expect = 5.4e-134
Identity = 249/279 (89.25%), Postives = 264/279 (94.62%), Query Frame = 0


Query: 93  SGIGLALAHQAASEGARVSILARSLNKLEDAKDAIRLSTGIDVAVYAADVRDYDAISRAA 152
           SGIGLALAHQAASEGARVSILARSL KLE+AKDAIRLSTGIDVAV+AADVRDYDAIS+A 
Sbjct: 48  SGIGLALAHQAASEGARVSILARSLKKLEEAKDAIRLSTGIDVAVFAADVRDYDAISKAV 107

Query: 153 DEAGPIDVLIVNQGVFVPQELAEQGMDEVKFMLDVNLVGTFNMIKAALPAMKNRVDRRPA 212
           DEAGPIDVL+VNQGVFVPQELAEQ MDEVKFMLDVNL+G F+MIKAALP MKNRVDRRPA
Sbjct: 108 DEAGPIDVLVVNQGVFVPQELAEQDMDEVKFMLDVNLLGAFHMIKAALPTMKNRVDRRPA 167

Query: 213 SIALMSSQAGQVGIYGYTAYSASKFGLRGLAEALQQEVIGDDIHISVIFPPDTDTPGLAE 272
           SIALMSSQAGQVG+YGYTAYSASKFGLRGLAEALQQEVIGD+IHIS+IFPPDTDTPGL E
Sbjct: 168 SIALMSSQAGQVGVYGYTAYSASKFGLRGLAEALQQEVIGDNIHISLIFPPDTDTPGLVE 227

Query: 273 EHKRRPELTSIIAAGGGSMKAEAVAKKTLNGIKSGSFIVPCNFEGALLSISTAGLSPQNS 332
           E KRRPELT+IIAAGGG MKAE VAKK LNGIKSGSFI+PCNFEGALLS++TAGLSPQ S
Sbjct: 228 EEKRRPELTNIIAAGGGRMKAELVAKKALNGIKSGSFIIPCNFEGALLSVATAGLSPQRS 287

Query: 333 FWMAFLEVVFAGIIRIVALCFQWVWYGSIEKWNAQKKRT 372
           F MAFLEVV AGIIRIV LCFQWVWY SI+KW+AQKKRT
Sbjct: 288 FLMAFLEVVTAGIIRIVGLCFQWVWYDSIQKWHAQKKRT 326

[Close]

BLAST of Moc05g06000 vs. ExPASy Swiss-Prot
Match: Q0WRJ2 (3-dehydrosphinganine reductase TSC10A OS=Arabidopsis thaliana OX=3702 GN=TSC10A PE=1 SV=1)

HSP 1 Score: 394.8 bits (1013), Expect = 1.1e-108
Identity = 194/278 (69.78%), Postives = 240/278 (86.33%), Query Frame = 0


Query: 93  SGIGLALAHQAASEGARVSILARSLNKLEDAKDAIRLSTGIDVAVYAADVRDYDAISRAA 152
           SGIGLALAH+AASEGARVSILARS +KLE+AK +I+L+TG++VA ++ADVRDYDA+S+A 
Sbjct: 49  SGIGLALAHRAASEGARVSILARSGSKLEEAKKSIQLATGVEVATFSADVRDYDAVSKAI 108

Query: 153 DEAGPIDVLIVNQGVFVPQELAEQGMDEVKFMLDVNLVGTFNMIKAALPAMKNRVDRRPA 212
           DE+GPIDVLIVNQGVF  +EL +   ++VKF +DVNLVG+FN+IKAALPAMK R DR PA
Sbjct: 109 DESGPIDVLIVNQGVFTAKELVKHSPEDVKFTIDVNLVGSFNVIKAALPAMKARKDRGPA 168

Query: 213 SIALMSSQAGQVGIYGYTAYSASKFGLRGLAEALQQEVIGDDIHISVIFPPDTDTPGLAE 272
           SI+L+SSQAGQVG+YGY AYSASKFGL+GLA+ALQQEVI DDIH+++IFPPDT+TPG  E
Sbjct: 169 SISLVSSQAGQVGVYGYAAYSASKFGLQGLAQALQQEVISDDIHVTLIFPPDTNTPGFEE 228

Query: 273 EHKRRPELTSIIAAGGGSMKAEAVAKKTLNGIKSGSFIVPCNFEGALLSISTAGLSPQNS 332
           E K RPE+T+IIAA  GSM+ E VAKK ++GIK+G+F V CNFEG LLS++T G+SPQ S
Sbjct: 229 EQKSRPEVTAIIAASSGSMETEEVAKKAMDGIKAGNFTVSCNFEGFLLSLATTGMSPQRS 288

Query: 333 FWMAFLEVVFAGIIRIVALCFQWVWYGSIEKWNAQKKR 371
           FW+AFLEV+ AG IR++AL FQW WY +IEKW+  K +
Sbjct: 289 FWLAFLEVITAGPIRLIALFFQWDWYKAIEKWSKTKTK 326

[Close]

BLAST of Moc05g06000 vs. ExPASy Swiss-Prot
Match: F4JZN6 (3-dehydrosphinganine reductase TSC10B OS=Arabidopsis thaliana OX=3702 GN=TSC10B PE=1 SV=1)

HSP 1 Score: 379.4 bits (973), Expect = 4.6e-104
Identity = 187/278 (67.27%), Postives = 232/278 (83.45%), Query Frame = 0


Query: 93  SGIGLALAHQAASEGARVSILARSLNKLEDAKDAIRLSTGIDVAVYAADVRDYDAISRAA 152
           SGIGLALAH+A SEGA+VSILARS  KL +AK +I+L+TG++VA ++ADVRDYDA+S+A 
Sbjct: 47  SGIGLALAHRAVSEGAKVSILARSTEKLAEAKRSIQLATGVEVATFSADVRDYDAVSKAI 106

Query: 153 DEAGPIDVLIVNQGVFVPQELAEQGMDEVKFMLDVNLVGTFNMIKAALPAMKNRVDRRPA 212
           DE+GPIDVLIVNQGVF+ +EL +Q  +EVKFM+DVNL G+FN+IKAALPAMK R  R PA
Sbjct: 107 DESGPIDVLIVNQGVFIGKELEKQSPEEVKFMIDVNLTGSFNVIKAALPAMKAREGRGPA 166

Query: 213 SIALMSSQAGQVGIYGYTAYSASKFGLRGLAEALQQEVIGDDIHISVIFPPDTDTPGLAE 272
           SI+L+SSQAGQ GIYGYTAYSASKFGL+GLA+ALQQEVI D IH++++FPPDTDTPG  +
Sbjct: 167 SISLVSSQAGQAGIYGYTAYSASKFGLQGLAQALQQEVISDGIHVTLLFPPDTDTPGFEQ 226

Query: 273 EHKRRPELTSIIAAGGGSMKAEAVAKKTLNGIKSGSFIVPCNFEGALLSISTAGLSPQNS 332
           E K+RPELTSIIAA  GSMK   VAK   +GIK+G F V C+F G LLSI++ G+SPQ S
Sbjct: 227 ELKKRPELTSIIAASSGSMKTNEVAKICFDGIKAGKFTVTCHFIGFLLSIASTGMSPQGS 286

Query: 333 FWMAFLEVVFAGIIRIVALCFQWVWYGSIEKWNAQKKR 371
           FW+A  EV+F G+IR+ +L FQW WY +IEKW+ + K+
Sbjct: 287 FWLALTEVMFGGLIRLASLVFQWQWYKTIEKWSQRNKK 324

[Close]

BLAST of Moc05g06000 vs. ExPASy Swiss-Prot
Match: Q06136 (3-ketodihydrosphingosine reductase OS=Homo sapiens OX=9606 GN=KDSR PE=1 SV=1)

HSP 1 Score: 159.8 bits (403), Expect = 5.8e-38
Identity = 99/287 (34.49%), Postives = 166/287 (57.84%), Query Frame = 0


Query: 93  SGIGLALAHQAASEGARVSILARSLNKLEDAKDAIRLSTGID---VAVYAADV-RDY--- 152
           SGIG  +A +   +GA ++++AR+ +KL  AK  I + +  D   V   + DV +DY   
Sbjct: 42  SGIGKCIAIECYKQGAFITLVARNEDKLLQAKKEIEMHSINDKQVVLCISVDVSQDYNQV 101

Query: 153 -DAISRAADEAGPIDVLIVNQGVFVPQELAEQGMDEVKFMLDVNLVGTFNMIKAALPAMK 212
            + I +A ++ GP+D+L+   G+ V  +  +  +   + ++ +N +G+    +A +  MK
Sbjct: 102 ENVIKQAQEKLGPVDMLVNCAGMAVSGKFEDLEVSTFERLMSINYLGSVYPSRAVITTMK 161

Query: 213 NRVDRRPASIALMSSQAGQVGIYGYTAYSASKFGLRGLAEALQQEVIGDDIHISVIFPPD 272
              +RR   I  +SSQAGQ+G++G+TAYSASKF +RGLAEALQ EV   +++I+V +PPD
Sbjct: 162 ---ERRVGRIVFVSSQAGQLGLFGFTAYSASKFAIRGLAEALQMEVKPYNVYITVAYPPD 221

Query: 273 TDTPGLAEEHKRRPELTSIIAAGGGSMKAEAVAKKTLNGIKSGSFIVPCNFEGALLSIST 332
           TDTPG AEE++ +P  T +I+      K E VAK+ +     G+F      +G +LS  T
Sbjct: 222 TDTPGFAEENRTKPLETRLISETTSVCKPEQVAKQIVKDAIQGNFNSSLGSDGYMLSALT 281

Query: 333 AGLSPQNSFWMAFLEVVFAGIIRIVALCFQWVWYGSIEKWNAQKKRT 372
            G++P  S      +VV  G+ R +AL +   +   + +   Q++++
Sbjct: 282 CGMAPVTSITEGLQQVVTMGLFRTIALFYLGSFDSIVRRCMMQREKS 325

[Close]

BLAST of Moc05g06000 vs. ExPASy Swiss-Prot
Match: Q6GV12 (3-ketodihydrosphingosine reductase OS=Mus musculus OX=10090 GN=Kdsr PE=1 SV=1)

HSP 1 Score: 159.1 bits (401), Expect = 9.8e-38
Identity = 100/286 (34.97%), Postives = 163/286 (56.99%), Query Frame = 0


Query: 93  SGIGLALAHQAASEGARVSILARSLNKLEDAKDAIRLSTGID---VAVYAADV-RDY--- 152
           SGIG  +A +   +GA ++++AR+ +KL  AK  I   +  D   V   + DV +DY   
Sbjct: 42  SGIGKCIAIECYKQGAFITLVARNEDKLLQAKKDIEKHSINDKQVVLCISVDVSQDYNQV 101

Query: 153 -DAISRAADEAGPIDVLIVNQGVFVPQELAEQGMDEVKFMLDVNLVGTFNMIKAALPAMK 212
            + I +A ++ GP+D+L+   G  +  +  E  +   + ++ +N +G+    +A +  MK
Sbjct: 102 ENVIKQAQEKLGPVDMLVNCAGTSMSGKFEELEVSSFEKLMSINYLGSVYPSRAVITTMK 161

Query: 213 NRVDRRPASIALMSSQAGQVGIYGYTAYSASKFGLRGLAEALQQEVIGDDIHISVIFPPD 272
              +RR   I  +SSQAGQ+G++G+TAYS+SKF +RGLAEALQ EV   +++++V +PPD
Sbjct: 162 ---ERRVGRIVFVSSQAGQLGLFGFTAYSSSKFAIRGLAEALQMEVKPYNVYVTVAYPPD 221

Query: 273 TDTPGLAEEHKRRPELTSIIAAGGGSMKAEAVAKKTLNGIKSGSFIVPCNFEGALLSIST 332
           TDTPGLAEE+K +P  T +I+      K E VAK+ +     G+F      +G +LS  T
Sbjct: 222 TDTPGLAEENKTKPLETRLISETTAICKPEQVAKQIVKDAIQGNFNSSIGSDGYMLSSLT 281

Query: 333 AGLSPQNSFWMAFLEVVFAGIIRIVALCFQWVWYGSIEKWNAQKKR 371
            G++P  S      +VV  G+ R +AL +   +   + +   QK +
Sbjct: 282 CGMAPVTSITEGLQQVVTMGLFRTIALFYLGSFDNIVRRCMVQKAK 324

[Close]

BLAST of Moc05g06000 vs. ExPASy Swiss-Prot
Match: Q2KIJ5 (3-ketodihydrosphingosine reductase OS=Bos taurus OX=9913 GN=KDSR PE=2 SV=1)

HSP 1 Score: 154.1 bits (388), Expect = 3.2e-36
Identity = 97/286 (33.92%), Postives = 165/286 (57.69%), Query Frame = 0


Query: 93  SGIGLALAHQAASEGARVSILARSLNKLEDAKDAIRLSTGID---VAVYAADV-RDY--- 152
           SGIG  +A +   +GA ++++AR+ +KL  AK  I   +  D   V   + DV +DY   
Sbjct: 42  SGIGKCIAIECYKQGAFITLVARNEDKLLQAKKEIEKHSINDKQVVLCISVDVSQDYSQV 101

Query: 153 -DAISRAADEAGPIDVLIVNQGVFVPQELAEQGMDEVKFMLDVNLVGTFNMIKAALPAMK 212
            + I +A ++ GP+D+L+   G+ +  +  +  +   + ++ +N +G+    +A +  MK
Sbjct: 102 ENVIKQAQEKLGPVDMLVNCAGMSLSGKFEDLEVSTFERLMSINYLGSVYPSRAVIATMK 161

Query: 213 NRVDRRPASIALMSSQAGQVGIYGYTAYSASKFGLRGLAEALQQEVIGDDIHISVIFPPD 272
              +RR   +  +SSQAGQ+G++GYTAYS+SKF LRGLAEALQ EV   +++++V +PPD
Sbjct: 162 ---ERRMGRVVFVSSQAGQLGLFGYTAYSSSKFALRGLAEALQMEVKPYNVYVTVAYPPD 221

Query: 273 TDTPGLAEEHKRRPELTSIIAAGGGSMKAEAVAKKTLNGIKSGSFIVPCNFEGALLSIST 332
           TDTPG A+E++ +P  T +I+      K E VAK+ +  ++ G+F      +G +LS  T
Sbjct: 222 TDTPGFAKENQTKPLETRLISETTSVCKPEQVAKQIVKDVQ-GNFNSSIGSDGYMLSSLT 281

Query: 333 AGLSPQNSFWMAFLEVVFAGIIRIVALCFQWVWYGSIEKWNAQKKR 371
            G++P  S      +VV  G+ R +AL +   +   + +   QK +
Sbjct: 282 CGMAPVTSIMEGLQQVVTMGLFRTIALFYLGSFDSIVRRCMMQKAK 323

[Close]

BLAST of Moc05g06000 vs. ExPASy TrEMBL
Match: A0A6J1C8X1 (3-dehydrosphinganine reductase TSC10A-like OS=Momordica charantia OX=3673 GN=LOC111009463 PE=4 SV=1)

HSP 1 Score: 540.8 bits (1392), Expect = 4.4e-150
Identity = 279/279 (100.00%), Postives = 279/279 (100.00%), Query Frame = 0


Query: 93  SGIGLALAHQAASEGARVSILARSLNKLEDAKDAIRLSTGIDVAVYAADVRDYDAISRAA 152
           SGIGLALAHQAASEGARVSILARSLNKLEDAKDAIRLSTGIDVAVYAADVRDYDAISRAA
Sbjct: 49  SGIGLALAHQAASEGARVSILARSLNKLEDAKDAIRLSTGIDVAVYAADVRDYDAISRAA 108

Query: 153 DEAGPIDVLIVNQGVFVPQELAEQGMDEVKFMLDVNLVGTFNMIKAALPAMKNRVDRRPA 212
           DEAGPIDVLIVNQGVFVPQELAEQGMDEVKFMLDVNLVGTFNMIKAALPAMKNRVDRRPA
Sbjct: 109 DEAGPIDVLIVNQGVFVPQELAEQGMDEVKFMLDVNLVGTFNMIKAALPAMKNRVDRRPA 168

Query: 213 SIALMSSQAGQVGIYGYTAYSASKFGLRGLAEALQQEVIGDDIHISVIFPPDTDTPGLAE 272
           SIALMSSQAGQVGIYGYTAYSASKFGLRGLAEALQQEVIGDDIHISVIFPPDTDTPGLAE
Sbjct: 169 SIALMSSQAGQVGIYGYTAYSASKFGLRGLAEALQQEVIGDDIHISVIFPPDTDTPGLAE 228

Query: 273 EHKRRPELTSIIAAGGGSMKAEAVAKKTLNGIKSGSFIVPCNFEGALLSISTAGLSPQNS 332
           EHKRRPELTSIIAAGGGSMKAEAVAKKTLNGIKSGSFIVPCNFEGALLSISTAGLSPQNS
Sbjct: 229 EHKRRPELTSIIAAGGGSMKAEAVAKKTLNGIKSGSFIVPCNFEGALLSISTAGLSPQNS 288

Query: 333 FWMAFLEVVFAGIIRIVALCFQWVWYGSIEKWNAQKKRT 372
           FWMAFLEVVFAGIIRIVALCFQWVWYGSIEKWNAQKKRT
Sbjct: 289 FWMAFLEVVFAGIIRIVALCFQWVWYGSIEKWNAQKKRT 327

[Close]

BLAST of Moc05g06000 vs. ExPASy TrEMBL
Match: A0A5D3D2X9 (3-dehydrosphinganine reductase TSC10A isoform X2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold434G00200 PE=4 SV=1)

HSP 1 Score: 492.7 bits (1267), Expect = 1.4e-135
Identity = 250/277 (90.25%), Postives = 266/277 (96.03%), Query Frame = 0


Query: 93  SGIGLALAHQAASEGARVSILARSLNKLEDAKDAIRLSTGIDVAVYAADVRDYDAISRAA 152
           SGIGLALAHQAA+EGARVSILARSL KLE+AKDAIRLSTGIDVAVYAADVRDYDAIS+A 
Sbjct: 48  SGIGLALAHQAATEGARVSILARSLKKLEEAKDAIRLSTGIDVAVYAADVRDYDAISKAV 107

Query: 153 DEAGPIDVLIVNQGVFVPQELAEQGMDEVKFMLDVNLVGTFNMIKAALPAMKNRVDRRPA 212
           DEAGPIDVLIVNQGVFVPQELAEQ +DEVKFMLDVNL+GTFNMIKAALPAMKNRVDRRPA
Sbjct: 108 DEAGPIDVLIVNQGVFVPQELAEQELDEVKFMLDVNLLGTFNMIKAALPAMKNRVDRRPA 167

Query: 213 SIALMSSQAGQVGIYGYTAYSASKFGLRGLAEALQQEVIGDDIHISVIFPPDTDTPGLAE 272
           SIALMSSQAGQVGIYGYTAYSASKFG+RGLAEALQQEVIGDDIHIS+IFPPDTDTPG AE
Sbjct: 168 SIALMSSQAGQVGIYGYTAYSASKFGIRGLAEALQQEVIGDDIHISLIFPPDTDTPGFAE 227

Query: 273 EHKRRPELTSIIAAGGGSMKAEAVAKKTLNGIKSGSFIVPCNFEGALLSISTAGLSPQNS 332
           E K+RPELT+IIAAGGGSMK E VAKK  NGIKSGSFI+PCNFEGALLS++TAGLSPQ S
Sbjct: 228 EQKKRPELTTIIAAGGGSMKPELVAKKAFNGIKSGSFIIPCNFEGALLSVATAGLSPQRS 287

Query: 333 FWMAFLEVVFAGIIRIVALCFQWVWYGSIEKWNAQKK 370
           F+MAFLEVV AG+IRIVALCFQWVWYGSIEKW+AQ+K
Sbjct: 288 FFMAFLEVVGAGVIRIVALCFQWVWYGSIEKWHAQRK 324

[Close]

BLAST of Moc05g06000 vs. ExPASy TrEMBL
Match: A0A1S4DT08 (3-dehydrosphinganine reductase TSC10A isoform X1 OS=Cucumis melo OX=3656 GN=LOC103483732 PE=4 SV=1)

HSP 1 Score: 492.7 bits (1267), Expect = 1.4e-135
Identity = 250/277 (90.25%), Postives = 266/277 (96.03%), Query Frame = 0


Query: 93  SGIGLALAHQAASEGARVSILARSLNKLEDAKDAIRLSTGIDVAVYAADVRDYDAISRAA 152
           SGIGLALAHQAA+EGARVSILARSL KLE+AKDAIRLSTGIDVAVYAADVRDYDAIS+A 
Sbjct: 48  SGIGLALAHQAATEGARVSILARSLKKLEEAKDAIRLSTGIDVAVYAADVRDYDAISKAV 107

Query: 153 DEAGPIDVLIVNQGVFVPQELAEQGMDEVKFMLDVNLVGTFNMIKAALPAMKNRVDRRPA 212
           DEAGPIDVLIVNQGVFVPQELAEQ +DEVKFMLDVNL+GTFNMIKAALPAMKNRVDRRPA
Sbjct: 108 DEAGPIDVLIVNQGVFVPQELAEQELDEVKFMLDVNLLGTFNMIKAALPAMKNRVDRRPA 167

Query: 213 SIALMSSQAGQVGIYGYTAYSASKFGLRGLAEALQQEVIGDDIHISVIFPPDTDTPGLAE 272
           SIALMSSQAGQVGIYGYTAYSASKFG+RGLAEALQQEVIGDDIHIS+IFPPDTDTPG AE
Sbjct: 168 SIALMSSQAGQVGIYGYTAYSASKFGIRGLAEALQQEVIGDDIHISLIFPPDTDTPGFAE 227

Query: 273 EHKRRPELTSIIAAGGGSMKAEAVAKKTLNGIKSGSFIVPCNFEGALLSISTAGLSPQNS 332
           E K+RPELT+IIAAGGGSMK E VAKK  NGIKSGSFI+PCNFEGALLS++TAGLSPQ S
Sbjct: 228 EQKKRPELTTIIAAGGGSMKPELVAKKAFNGIKSGSFIIPCNFEGALLSVATAGLSPQRS 287

Query: 333 FWMAFLEVVFAGIIRIVALCFQWVWYGSIEKWNAQKK 370
           F+MAFLEVV AG+IRIVALCFQWVWYGSIEKW+AQ+K
Sbjct: 288 FFMAFLEVVGAGVIRIVALCFQWVWYGSIEKWHAQRK 324

[Close]

BLAST of Moc05g06000 vs. ExPASy TrEMBL
Match: A0A1S3AX60 (3-dehydrosphinganine reductase TSC10A isoform X2 OS=Cucumis melo OX=3656 GN=LOC103483732 PE=4 SV=1)

HSP 1 Score: 492.7 bits (1267), Expect = 1.4e-135
Identity = 250/277 (90.25%), Postives = 266/277 (96.03%), Query Frame = 0


Query: 93  SGIGLALAHQAASEGARVSILARSLNKLEDAKDAIRLSTGIDVAVYAADVRDYDAISRAA 152
           SGIGLALAHQAA+EGARVSILARSL KLE+AKDAIRLSTGIDVAVYAADVRDYDAIS+A 
Sbjct: 48  SGIGLALAHQAATEGARVSILARSLKKLEEAKDAIRLSTGIDVAVYAADVRDYDAISKAV 107

Query: 153 DEAGPIDVLIVNQGVFVPQELAEQGMDEVKFMLDVNLVGTFNMIKAALPAMKNRVDRRPA 212
           DEAGPIDVLIVNQGVFVPQELAEQ +DEVKFMLDVNL+GTFNMIKAALPAMKNRVDRRPA
Sbjct: 108 DEAGPIDVLIVNQGVFVPQELAEQELDEVKFMLDVNLLGTFNMIKAALPAMKNRVDRRPA 167

Query: 213 SIALMSSQAGQVGIYGYTAYSASKFGLRGLAEALQQEVIGDDIHISVIFPPDTDTPGLAE 272
           SIALMSSQAGQVGIYGYTAYSASKFG+RGLAEALQQEVIGDDIHIS+IFPPDTDTPG AE
Sbjct: 168 SIALMSSQAGQVGIYGYTAYSASKFGIRGLAEALQQEVIGDDIHISLIFPPDTDTPGFAE 227

Query: 273 EHKRRPELTSIIAAGGGSMKAEAVAKKTLNGIKSGSFIVPCNFEGALLSISTAGLSPQNS 332
           E K+RPELT+IIAAGGGSMK E VAKK  NGIKSGSFI+PCNFEGALLS++TAGLSPQ S
Sbjct: 228 EQKKRPELTTIIAAGGGSMKPELVAKKAFNGIKSGSFIIPCNFEGALLSVATAGLSPQRS 287

Query: 333 FWMAFLEVVFAGIIRIVALCFQWVWYGSIEKWNAQKK 370
           F+MAFLEVV AG+IRIVALCFQWVWYGSIEKW+AQ+K
Sbjct: 288 FFMAFLEVVGAGVIRIVALCFQWVWYGSIEKWHAQRK 324

[Close]

BLAST of Moc05g06000 vs. ExPASy TrEMBL
Match: A0A0A0L506 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G149900 PE=4 SV=1)

HSP 1 Score: 490.0 bits (1260), Expect = 9.0e-135
Identity = 248/279 (88.89%), Postives = 265/279 (94.98%), Query Frame = 0


Query: 93  SGIGLALAHQAASEGARVSILARSLNKLEDAKDAIRLSTGIDVAVYAADVRDYDAISRAA 152
           SGIGLALAHQAA+EGARVSILARSL KLE+AKDAIRLSTGIDVAVYAADVRDYDAI +A 
Sbjct: 48  SGIGLALAHQAATEGARVSILARSLKKLEEAKDAIRLSTGIDVAVYAADVRDYDAILKAV 107

Query: 153 DEAGPIDVLIVNQGVFVPQELAEQGMDEVKFMLDVNLVGTFNMIKAALPAMKNRVDRRPA 212
           DEAGPIDVLIVNQGVFVPQELAEQ +DEVKFMLDVNL+GTFNMIKAALPAMKNRVDRRPA
Sbjct: 108 DEAGPIDVLIVNQGVFVPQELAEQELDEVKFMLDVNLLGTFNMIKAALPAMKNRVDRRPA 167

Query: 213 SIALMSSQAGQVGIYGYTAYSASKFGLRGLAEALQQEVIGDDIHISVIFPPDTDTPGLAE 272
           SIALMSSQAGQVGIYGYTAYSASKFG+RGLAEALQQEVIGDDIH+S+IFPPDTDTPG AE
Sbjct: 168 SIALMSSQAGQVGIYGYTAYSASKFGIRGLAEALQQEVIGDDIHVSLIFPPDTDTPGFAE 227

Query: 273 EHKRRPELTSIIAAGGGSMKAEAVAKKTLNGIKSGSFIVPCNFEGALLSISTAGLSPQNS 332
           E K+RPELT+IIAAGGGSMK E VAKK  NGIKSGSFI+PCNFEG LLSI+TAGLSPQ S
Sbjct: 228 EQKKRPELTTIIAAGGGSMKPELVAKKAFNGIKSGSFIIPCNFEGTLLSIATAGLSPQRS 287

Query: 333 FWMAFLEVVFAGIIRIVALCFQWVWYGSIEKWNAQKKRT 372
           F+MAF+EVV AG+IRIVALCFQWVWYGSIEKW+AQ+ RT
Sbjct: 288 FFMAFIEVVGAGVIRIVALCFQWVWYGSIEKWHAQRNRT 326

[Close]

BLAST of Moc05g06000 vs. TAIR 10
Match: AT3G06060.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 394.8 bits (1013), Expect = 7.6e-110
Identity = 194/278 (69.78%), Postives = 240/278 (86.33%), Query Frame = 0


Query: 93  SGIGLALAHQAASEGARVSILARSLNKLEDAKDAIRLSTGIDVAVYAADVRDYDAISRAA 152
           SGIGLALAH+AASEGARVSILARS +KLE+AK +I+L+TG++VA ++ADVRDYDA+S+A 
Sbjct: 49  SGIGLALAHRAASEGARVSILARSGSKLEEAKKSIQLATGVEVATFSADVRDYDAVSKAI 108

Query: 153 DEAGPIDVLIVNQGVFVPQELAEQGMDEVKFMLDVNLVGTFNMIKAALPAMKNRVDRRPA 212
           DE+GPIDVLIVNQGVF  +EL +   ++VKF +DVNLVG+FN+IKAALPAMK R DR PA
Sbjct: 109 DESGPIDVLIVNQGVFTAKELVKHSPEDVKFTIDVNLVGSFNVIKAALPAMKARKDRGPA 168

Query: 213 SIALMSSQAGQVGIYGYTAYSASKFGLRGLAEALQQEVIGDDIHISVIFPPDTDTPGLAE 272
           SI+L+SSQAGQVG+YGY AYSASKFGL+GLA+ALQQEVI DDIH+++IFPPDT+TPG  E
Sbjct: 169 SISLVSSQAGQVGVYGYAAYSASKFGLQGLAQALQQEVISDDIHVTLIFPPDTNTPGFEE 228

Query: 273 EHKRRPELTSIIAAGGGSMKAEAVAKKTLNGIKSGSFIVPCNFEGALLSISTAGLSPQNS 332
           E K RPE+T+IIAA  GSM+ E VAKK ++GIK+G+F V CNFEG LLS++T G+SPQ S
Sbjct: 229 EQKSRPEVTAIIAASSGSMETEEVAKKAMDGIKAGNFTVSCNFEGFLLSLATTGMSPQRS 288

Query: 333 FWMAFLEVVFAGIIRIVALCFQWVWYGSIEKWNAQKKR 371
           FW+AFLEV+ AG IR++AL FQW WY +IEKW+  K +
Sbjct: 289 FWLAFLEVITAGPIRLIALFFQWDWYKAIEKWSKTKTK 326

[Close]

BLAST of Moc05g06000 vs. TAIR 10
Match: AT5G19200.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 379.4 bits (973), Expect = 3.3e-105
Identity = 187/278 (67.27%), Postives = 232/278 (83.45%), Query Frame = 0


Query: 93  SGIGLALAHQAASEGARVSILARSLNKLEDAKDAIRLSTGIDVAVYAADVRDYDAISRAA 152
           SGIGLALAH+A SEGA+VSILARS  KL +AK +I+L+TG++VA ++ADVRDYDA+S+A 
Sbjct: 47  SGIGLALAHRAVSEGAKVSILARSTEKLAEAKRSIQLATGVEVATFSADVRDYDAVSKAI 106

Query: 153 DEAGPIDVLIVNQGVFVPQELAEQGMDEVKFMLDVNLVGTFNMIKAALPAMKNRVDRRPA 212
           DE+GPIDVLIVNQGVF+ +EL +Q  +EVKFM+DVNL G+FN+IKAALPAMK R  R PA
Sbjct: 107 DESGPIDVLIVNQGVFIGKELEKQSPEEVKFMIDVNLTGSFNVIKAALPAMKAREGRGPA 166

Query: 213 SIALMSSQAGQVGIYGYTAYSASKFGLRGLAEALQQEVIGDDIHISVIFPPDTDTPGLAE 272
           SI+L+SSQAGQ GIYGYTAYSASKFGL+GLA+ALQQEVI D IH++++FPPDTDTPG  +
Sbjct: 167 SISLVSSQAGQAGIYGYTAYSASKFGLQGLAQALQQEVISDGIHVTLLFPPDTDTPGFEQ 226

Query: 273 EHKRRPELTSIIAAGGGSMKAEAVAKKTLNGIKSGSFIVPCNFEGALLSISTAGLSPQNS 332
           E K+RPELTSIIAA  GSMK   VAK   +GIK+G F V C+F G LLSI++ G+SPQ S
Sbjct: 227 ELKKRPELTSIIAASSGSMKTNEVAKICFDGIKAGKFTVTCHFIGFLLSIASTGMSPQGS 286

Query: 333 FWMAFLEVVFAGIIRIVALCFQWVWYGSIEKWNAQKKR 371
           FW+A  EV+F G+IR+ +L FQW WY +IEKW+ + K+
Sbjct: 287 FWLALTEVMFGGLIRLASLVFQWQWYKTIEKWSQRNKK 324

[Close]

BLAST of Moc05g06000 vs. TAIR 10
Match: AT5G50600.1 (hydroxysteroid dehydrogenase 1 )

HSP 1 Score: 64.3 bits (155), Expect = 2.3e-10
Identity = 64/224 (28.57%), Postives = 101/224 (45.09%), Query Frame = 0


Query: 93  SGIGLALAHQAASEGARVSILARSLNKLEDAKDAIRLSTGIDVAVYAADVRDYDAISRAA 152
           SGIG  LA++ A  GA +++ AR  N+LE+  +  R     +V    ADV   D   R  
Sbjct: 57  SGIGEQLAYEYACRGACLALTARRKNRLEEVAEIARELGSPNVVTVHADVSKPDDCRRIV 116

Query: 153 DEA----GPIDVLIVNQGVFVPQELAE-QGMDEVKFMLDVNLVGTFNMIKAALPAMKNRV 212
           D+     G +D L+ N G+         + +   K +LD N  G+    +AALP ++   
Sbjct: 117 DDTITHFGRLDHLVNNAGMTQISMFENIEDITRTKAVLDTNFWGSVYTTRAALPYLR--- 176

Query: 213 DRRPASIALMSSQAGQVGIYGYTAYSASKFGLRGLAEALQQEVIGDDIHISVIFPPDTDT 272
            +    I  MSS A  +     + Y+ASK  L    E ++ E +G D+HI+++      T
Sbjct: 177 -QSNGKIVAMSSSAAWLTAPRMSFYNASKAALLSFFETMRIE-LGGDVHITIV------T 236

Query: 273 PGLAEEHKRRPELTSIIAAGGGSMKAEAVAKKTLNGIKSGSFIV 312
           PG  E      ELT      G   + E +  + +  ++ G F V
Sbjct: 237 PGYIES-----ELTQGKYFSG---EGELIVNQDMRDVQVGPFPV 261

[Close]

BLAST of Moc05g06000 vs. TAIR 10
Match: AT5G50700.1 (hydroxysteroid dehydrogenase 1 )

HSP 1 Score: 64.3 bits (155), Expect = 2.3e-10
Identity = 64/224 (28.57%), Postives = 101/224 (45.09%), Query Frame = 0


Query: 93  SGIGLALAHQAASEGARVSILARSLNKLEDAKDAIRLSTGIDVAVYAADVRDYDAISRAA 152
           SGIG  LA++ A  GA +++ AR  N+LE+  +  R     +V    ADV   D   R  
Sbjct: 57  SGIGEQLAYEYACRGACLALTARRKNRLEEVAEIARELGSPNVVTVHADVSKPDDCRRIV 116

Query: 153 DEA----GPIDVLIVNQGVFVPQELAE-QGMDEVKFMLDVNLVGTFNMIKAALPAMKNRV 212
           D+     G +D L+ N G+         + +   K +LD N  G+    +AALP ++   
Sbjct: 117 DDTITHFGRLDHLVNNAGMTQISMFENIEDITRTKAVLDTNFWGSVYTTRAALPYLR--- 176

Query: 213 DRRPASIALMSSQAGQVGIYGYTAYSASKFGLRGLAEALQQEVIGDDIHISVIFPPDTDT 272
            +    I  MSS A  +     + Y+ASK  L    E ++ E +G D+HI+++      T
Sbjct: 177 -QSNGKIVAMSSSAAWLTAPRMSFYNASKAALLSFFETMRIE-LGGDVHITIV------T 236

Query: 273 PGLAEEHKRRPELTSIIAAGGGSMKAEAVAKKTLNGIKSGSFIV 312
           PG  E      ELT      G   + E +  + +  ++ G F V
Sbjct: 237 PGYIES-----ELTQGKYFSG---EGELIVNQDMRDVQVGPFPV 261

[Close]

BLAST of Moc05g06000 vs. TAIR 10
Match: AT2G29370.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 61.2 bits (147), Expect = 2.0e-09
Identity = 51/180 (28.33%), Postives = 82/180 (45.56%), Query Frame = 0


Query: 94  GIGLALAHQAASEGARVSILARSLNKLEDAKDAIRLSTGIDVAVYAADVRDYDAISRAAD 153
           G+G A+  + A  GARV   AR   +L+++    + + G+ V     DV   D   +  +
Sbjct: 29  GLGKAVVEELAMLGARVHTCARDETQLQESLREWQ-AKGLQVTTSVCDVSSRDQREKLME 88

Query: 154 EA-----GPIDVLIVNQGVFVPQELAEQGMDEVKFMLDVNLVGTFNMIKAALPAMKNRVD 213
                  G + +L+ N G+ V +   E   +E  F++  NL  TF+  + A P +K    
Sbjct: 89  TVSSLFQGKLSILVPNVGIGVLKPTTECTAEEFSFIIATNLESTFHFSQLAHPLLK---A 148

Query: 214 RRPASIALMSSQAGQVGIYGYTAYSASKFGLRGLAEALQQEVIGDDIHISVIFPPDTDTP 269
               +I LMSS AG V +   + Y A+K  +  LA  L  E   D+I  + + P    TP
Sbjct: 149 SGSGNIVLMSSVAGVVNLGNTSIYGATKGAMNQLARNLACEWASDNIRANSVCPWFITTP 204

The following BLAST results are available for this feature:

BLAST of Moc05g06000 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022138240.1	9.2e-150	100.00	3-dehydrosphinganine reductase TSC10A-like [Momordica charantia]	[more]
XP_016899126.1	2.9e-135	90.25	PREDICTED: 3-dehydrosphinganine reductase TSC10A isoform X1 [Cucumis melo]	[more]
XP_008438707.1	2.9e-135	90.25	PREDICTED: 3-dehydrosphinganine reductase TSC10A isoform X2 [Cucumis melo] >KAA0...	[more]
XP_004134147.1	1.9e-134	88.89	3-dehydrosphinganine reductase TSC10A [Cucumis sativus] >KGN57010.1 hypothetical...	[more]
XP_023539235.1	5.4e-134	89.25	3-dehydrosphinganine reductase TSC10A-like [Cucurbita pepo subsp. pepo]	[more]

BLAST of Moc05g06000 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q0WRJ2	1.1e-108	69.78	3-dehydrosphinganine reductase TSC10A OS=Arabidopsis thaliana OX=3702 GN=TSC10A ...	[more]
F4JZN6	4.6e-104	67.27	3-dehydrosphinganine reductase TSC10B OS=Arabidopsis thaliana OX=3702 GN=TSC10B ...	[more]
Q06136	5.8e-38	34.49	3-ketodihydrosphingosine reductase OS=Homo sapiens OX=9606 GN=KDSR PE=1 SV=1	[more]
Q6GV12	9.8e-38	34.97	3-ketodihydrosphingosine reductase OS=Mus musculus OX=10090 GN=Kdsr PE=1 SV=1	[more]
Q2KIJ5	3.2e-36	33.92	3-ketodihydrosphingosine reductase OS=Bos taurus OX=9913 GN=KDSR PE=2 SV=1	[more]

BLAST of Moc05g06000 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1C8X1	4.4e-150	100.00	3-dehydrosphinganine reductase TSC10A-like OS=Momordica charantia OX=3673 GN=LOC...	[more]
A0A5D3D2X9	1.4e-135	90.25	3-dehydrosphinganine reductase TSC10A isoform X2 OS=Cucumis melo var. makuwa OX=...	[more]
A0A1S4DT08	1.4e-135	90.25	3-dehydrosphinganine reductase TSC10A isoform X1 OS=Cucumis melo OX=3656 GN=LOC1...	[more]
A0A1S3AX60	1.4e-135	90.25	3-dehydrosphinganine reductase TSC10A isoform X2 OS=Cucumis melo OX=3656 GN=LOC1...	[more]
A0A0A0L506	9.0e-135	88.89	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G149900 PE=4 SV=1	[more]

BLAST of Moc05g06000 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G06060.1	7.6e-110	69.78	NAD(P)-binding Rossmann-fold superfamily protein	[more]
AT5G19200.1	3.3e-105	67.27	NAD(P)-binding Rossmann-fold superfamily protein	[more]
AT5G50600.1	2.3e-10	28.57	hydroxysteroid dehydrogenase 1	[more]
AT5G50700.1	2.3e-10	28.57	hydroxysteroid dehydrogenase 1	[more]
AT2G29370.1	2.0e-09	28.33	NAD(P)-binding Rossmann-fold superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (OHB3-1) v2
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002347	Short-chain dehydrogenase/reductase SDR	PRINTS	PR00081	GDHRDH	coord: 232..251 score: 34.62 coord: 156..167 score: 45.43 coord: 206..222 score: 23.5
IPR002347	Short-chain dehydrogenase/reductase SDR	PFAM	PF00106	adh_short	coord: 93..275 e-value: 7.6E-39 score: 133.2
None	No IPR available	GENE3D	3.40.50.720		coord: 92..318 e-value: 2.6E-50 score: 173.2
None	No IPR available	PIRSR	PIRSR000095-1	PIRSR000095-1	coord: 93..262 e-value: 1.0E-25 score: 88.3
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 1..32
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 17..31
None	No IPR available	PANTHER	PTHR43550:SF8	3-DEHYDROSPHINGANINE REDUCTASE TSC10A	coord: 92..369
None	No IPR available	PANTHER	PTHR43550	3-KETODIHYDROSPHINGOSINE REDUCTASE	coord: 92..369
IPR020904	Short-chain dehydrogenase/reductase, conserved site	PROSITE	PS00061	ADH_SHORT	coord: 219..247
IPR045022	3-ketodihydrosphingosine reductase KDSR-like	CDD	cd08939	KDSR-like_SDR_c	coord: 93..317 e-value: 1.09406E-80 score: 244.855
IPR036291	NAD(P)-binding domain superfamily	SUPERFAMILY	51735	NAD(P)-binding Rossmann-fold domains	coord: 93..311

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Moc05g06000.1	Moc05g06000.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006666	3-keto-sphinganine metabolic process
biological_process	GO:0030148	sphingolipid biosynthetic process
cellular_component	GO:0005789	endoplasmic reticulum membrane
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0047560	3-dehydrosphinganine reductase activity
molecular_function	GO:0016491	oxidoreductase activity