Moc05g04890 (gene) Bitter gourd (OHB3-1) v2

Overview
NameMoc05g04890
Typegene
OrganismMomordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
DescriptionDNA-directed RNA polymerase II subunit 1-like
Locationchr5: 3360368 .. 3362031 (+)
RNA-Seq ExpressionMoc05g04890
SyntenyMoc05g04890
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCAAATCTTCCTCGGTTTGGCCGCACATGGCAACGTTTCTCTTCCCTCCCCCGCCCCGCCACTGCCTCACAACCGGAACAACTTCAGCCGGCACCAGCGCCGGCGCCGATGAATGGGCGTGAAATTTCACCCAACTCTCCGGTCACCGCCCAAGTTCTCCAAACTTCTCCAATTAGAGAAAGATCTTCTCGACTTCCATCTCCCACGAAGAAATTTGCCTCGCCGCCTTCTTCTCCAAAATATAGACCCGCCGCCGCCGCTTCGCCTCCCAAGCCACTGTCTCCAACTCCGTCGTACAACCGTTACGACGGCGAACGACGGTCTAGTGCCACCACATCTCCCAAAGCCATTAAACCCTCCTATACCTCTCCGCCGCTGTCGCCTGCCAAGCACAAATACCCAACCGCCGCCACCGCCGCACCGCTCTCTCCTCTAACTCTGCCTCGGTCGGAGGCGAAACATGAACCTGGGCCCACGATTCGATCCAGAAGCCCACCGGAGGTTAGTTAACTTTAAATATTACGATATATATTAATTTCTTTTTACGTAAATACTACTTTTATTGGTATAATTTAATTATTTTGATTATTGTGCTATCAAAATGTCAATTTTTTTTGGTCTACTTTCCATTTTTATTCGTTTTAGTTATCATAATTTTTAAAAGTCCCTTTTAATTTTTCATATTTTTTTTATAAAGGGACCATTTTAATTTTTTTTTGCTATGACTGAATAAATTACCTCCCAAATCAAAAGTTTAAACTCATGATTTTGATAAATTTAATTATATATATGGTCGAAAATTTTATTCGAGTTCTTTTCTGTCACAATTTTAGCGTCATATTTTATTTACTAATTTTAAAAAAATGTAATCATAAATTTGTATTAAAATATTTTTATTCCATAAGATATGTGTTGAAATGTTGCAACAAAGAACAAAAGATGTTCAACTTTAAAAAGTAAACTAAAACGGACCTATCAAGATTTATAAGATATATGAACTATGATACTCCTTGACCAAATGATTTTGAGATGACACTCGACGATATTAGTATTAGAGTTGAACAAGTTTAAACGACCATCCAAGCAAAAAAATGAGATTCAACCAACATTAATGTATCCATAATTTACGTGTGTATGAGTTTAACAAGATGACTTTCTCTTGTTTTGTTCCAATCAAATTATAGTTTATCATTGAATAATTCAGGTTAGTTCTGGTATATATTTAATCTTGTATACATATTCTTAATCAGGTCGAGCAGAAATCGATACATTATCCGAAGGTCGAGAAGCCGACGAAACCCGATCACCGGCCGTCAGAGTACAACTCCGGCAAGCCCCAATACAAGCAGCAGCAGCAACAGAGCGACGTGATAACCATCAAAGGCGAGAACGTAGGCGCCATCATGCACATAACTCAATCATCTGACGGCACAGAAATGGTGAAGAAAAAGCCAAGCACAGAGAGTGGAAACGATGATGAGAAAGGCAACAAATCAAGTTCTTTGCCGGCGAAATCATTCATGAACAGCAATTTTCAAGGGGTCAATAATTCCGTTCTGTACAACTCCTCCTTCAGCCACCGTGATCCAGGCCTGCACCTGTCTTTCTCCAAGAAGCCCGCCCATGGCCATGCCCAATCTGTTCTTGGTTCGACCTACTAG

mRNA sequence

ATGGCAAATCTTCCTCGGTTTGGCCGCACATGGCAACGTTTCTCTTCCCTCCCCCGCCCCGCCACTGCCTCACAACCGGAACAACTTCAGCCGGCACCAGCGCCGGCGCCGATGAATGGGCGTGAAATTTCACCCAACTCTCCGGTCACCGCCCAAGTTCTCCAAACTTCTCCAATTAGAGAAAGATCTTCTCGACTTCCATCTCCCACGAAGAAATTTGCCTCGCCGCCTTCTTCTCCAAAATATAGACCCGCCGCCGCCGCTTCGCCTCCCAAGCCACTGTCTCCAACTCCGTCGTACAACCGTTACGACGGCGAACGACGGTCTAGTGCCACCACATCTCCCAAAGCCATTAAACCCTCCTATACCTCTCCGCCGCTGTCGCCTGCCAAGCACAAATACCCAACCGCCGCCACCGCCGCACCGCTCTCTCCTCTAACTCTGCCTCGGTCGGAGGCGAAACATGAACCTGGGCCCACGATTCGATCCAGAAGCCCACCGGAGGTCGAGCAGAAATCGATACATTATCCGAAGGTCGAGAAGCCGACGAAACCCGATCACCGGCCGTCAGAGTACAACTCCGGCAAGCCCCAATACAAGCAGCAGCAGCAACAGAGCGACGTGATAACCATCAAAGGCGAGAACGTAGGCGCCATCATGCACATAACTCAATCATCTGACGGCACAGAAATGGTGAAGAAAAAGCCAAGCACAGAGAGTGGAAACGATGATGAGAAAGGCAACAAATCAAGTTCTTTGCCGGCGAAATCATTCATGAACAGCAATTTTCAAGGGGTCAATAATTCCGTTCTGTACAACTCCTCCTTCAGCCACCGTGATCCAGGCCTGCACCTGTCTTTCTCCAAGAAGCCCGCCCATGGCCATGCCCAATCTGTTCTTGGTTCGACCTACTAG

Coding sequence (CDS)

ATGGCAAATCTTCCTCGGTTTGGCCGCACATGGCAACGTTTCTCTTCCCTCCCCCGCCCCGCCACTGCCTCACAACCGGAACAACTTCAGCCGGCACCAGCGCCGGCGCCGATGAATGGGCGTGAAATTTCACCCAACTCTCCGGTCACCGCCCAAGTTCTCCAAACTTCTCCAATTAGAGAAAGATCTTCTCGACTTCCATCTCCCACGAAGAAATTTGCCTCGCCGCCTTCTTCTCCAAAATATAGACCCGCCGCCGCCGCTTCGCCTCCCAAGCCACTGTCTCCAACTCCGTCGTACAACCGTTACGACGGCGAACGACGGTCTAGTGCCACCACATCTCCCAAAGCCATTAAACCCTCCTATACCTCTCCGCCGCTGTCGCCTGCCAAGCACAAATACCCAACCGCCGCCACCGCCGCACCGCTCTCTCCTCTAACTCTGCCTCGGTCGGAGGCGAAACATGAACCTGGGCCCACGATTCGATCCAGAAGCCCACCGGAGGTCGAGCAGAAATCGATACATTATCCGAAGGTCGAGAAGCCGACGAAACCCGATCACCGGCCGTCAGAGTACAACTCCGGCAAGCCCCAATACAAGCAGCAGCAGCAACAGAGCGACGTGATAACCATCAAAGGCGAGAACGTAGGCGCCATCATGCACATAACTCAATCATCTGACGGCACAGAAATGGTGAAGAAAAAGCCAAGCACAGAGAGTGGAAACGATGATGAGAAAGGCAACAAATCAAGTTCTTTGCCGGCGAAATCATTCATGAACAGCAATTTTCAAGGGGTCAATAATTCCGTTCTGTACAACTCCTCCTTCAGCCACCGTGATCCAGGCCTGCACCTGTCTTTCTCCAAGAAGCCCGCCCATGGCCATGCCCAATCTGTTCTTGGTTCGACCTACTAG

Protein sequence

MANLPRFGRTWQRFSSLPRPATASQPEQLQPAPAPAPMNGREISPNSPVTAQVLQTSPIRERSSRLPSPTKKFASPPSSPKYRPAAAASPPKPLSPTPSYNRYDGERRSSATTSPKAIKPSYTSPPLSPAKHKYPTAATAAPLSPLTLPRSEAKHEPGPTIRSRSPPEVEQKSIHYPKVEKPTKPDHRPSEYNSGKPQYKQQQQQSDVITIKGENVGAIMHITQSSDGTEMVKKKPSTESGNDDEKGNKSSSLPAKSFMNSNFQGVNNSVLYNSSFSHRDPGLHLSFSKKPAHGHAQSVLGSTY
Homology
BLAST of Moc05g04890 vs. NCBI nr
Match: XP_022147442.1 (proline-rich receptor-like protein kinase PERK2 [Momordica charantia])

HSP 1 Score: 581.6 bits (1498), Expect = 3.8e-162
Identity = 304/304 (100.00%), Postives = 304/304 (100.00%), Query Frame = 0

Query: 1   MANLPRFGRTWQRFSSLPRPATASQPEQLQPAPAPAPMNGREISPNSPVTAQVLQTSPIR 60
           MANLPRFGRTWQRFSSLPRPATASQPEQLQPAPAPAPMNGREISPNSPVTAQVLQTSPIR
Sbjct: 1   MANLPRFGRTWQRFSSLPRPATASQPEQLQPAPAPAPMNGREISPNSPVTAQVLQTSPIR 60

Query: 61  ERSSRLPSPTKKFASPPSSPKYRPAAAASPPKPLSPTPSYNRYDGERRSSATTSPKAIKP 120
           ERSSRLPSPTKKFASPPSSPKYRPAAAASPPKPLSPTPSYNRYDGERRSSATTSPKAIKP
Sbjct: 61  ERSSRLPSPTKKFASPPSSPKYRPAAAASPPKPLSPTPSYNRYDGERRSSATTSPKAIKP 120

Query: 121 SYTSPPLSPAKHKYPTAATAAPLSPLTLPRSEAKHEPGPTIRSRSPPEVEQKSIHYPKVE 180
           SYTSPPLSPAKHKYPTAATAAPLSPLTLPRSEAKHEPGPTIRSRSPPEVEQKSIHYPKVE
Sbjct: 121 SYTSPPLSPAKHKYPTAATAAPLSPLTLPRSEAKHEPGPTIRSRSPPEVEQKSIHYPKVE 180

Query: 181 KPTKPDHRPSEYNSGKPQYKQQQQQSDVITIKGENVGAIMHITQSSDGTEMVKKKPSTES 240
           KPTKPDHRPSEYNSGKPQYKQQQQQSDVITIKGENVGAIMHITQSSDGTEMVKKKPSTES
Sbjct: 181 KPTKPDHRPSEYNSGKPQYKQQQQQSDVITIKGENVGAIMHITQSSDGTEMVKKKPSTES 240

Query: 241 GNDDEKGNKSSSLPAKSFMNSNFQGVNNSVLYNSSFSHRDPGLHLSFSKKPAHGHAQSVL 300
           GNDDEKGNKSSSLPAKSFMNSNFQGVNNSVLYNSSFSHRDPGLHLSFSKKPAHGHAQSVL
Sbjct: 241 GNDDEKGNKSSSLPAKSFMNSNFQGVNNSVLYNSSFSHRDPGLHLSFSKKPAHGHAQSVL 300

Query: 301 GSTY 305
           GSTY
Sbjct: 301 GSTY 304

BLAST of Moc05g04890 vs. NCBI nr
Match: XP_022945677.1 (DNA-directed RNA polymerase II subunit 1-like [Cucurbita moschata])

HSP 1 Score: 355.1 bits (910), Expect = 5.8e-94
Identity = 202/299 (67.56%), Postives = 231/299 (77.26%), Query Frame = 0

Query: 1   MANLPRFGRTWQRFSSLPRPATASQPEQLQPAPAPAPMNGREISPNSPVTAQVLQTSPIR 60
           MANLPRFGRTW RFSSLPRP TA + +     P PA  +  E+ P++P TA VLQTSPI+
Sbjct: 1   MANLPRFGRTWNRFSSLPRPGTAPRLD----VPPPAATSEPEVYPSAPRTANVLQTSPIK 60

Query: 61  ERSSRLPSPTKKFASPPSSPKYRPAA-AASPPKPLSPTPSYNRYDGERRSSATTSPKAIK 120
           ERS R+ SP +K+ SPP+SPKYR AA + SP KPLSP P+YNRYDGERRSSA  SPK  K
Sbjct: 61  ERSPRITSPVRKYPSPPTSPKYRAAAPSTSPRKPLSPPPTYNRYDGERRSSALASPKTFK 120

Query: 121 PSYTSPPLSPAKHKYPTAATAAPLSPLTLPRSEAKHEPGPTIRSRSPPEVEQKSIHYPKV 180
            +YTSPP SPAKHKY T+   APLSPL LP  E +HEP P +R RSPPEV+QKS+ Y K 
Sbjct: 121 TTYTSPPRSPAKHKYSTSTVQAPLSPLALPSLEKRHEPEPVVRPRSPPEVQQKSVLYQKT 180

Query: 181 --EKPTKPDHRPSEYNSGKPQYKQ-QQQQSDVITIKGENVGAIMHITQSSDGTEMVKKKP 240
             EKP K DHR SEY+SGKPQ KQ  QQQSDVI IKGENVGA+MHITQSSD TE  KKKP
Sbjct: 181 TSEKPAKTDHRASEYSSGKPQQKQTHQQQSDVINIKGENVGAVMHITQSSDATETHKKKP 240

Query: 241 STESGNDDEK-GNKSSS-LPAKSFMNSNFQGVNNSVLYNSSFSHRDPGLHLSFSKKPAH 294
           +    +++EK  NKSSS +P KSFMNSNFQGVNNS+LYNSS SHRDPGLHL+F+KKP H
Sbjct: 241 TVSYSSENEKDSNKSSSNMPGKSFMNSNFQGVNNSILYNSSLSHRDPGLHLAFTKKPTH 295

BLAST of Moc05g04890 vs. NCBI nr
Match: KAG6597058.1 (hypothetical protein SDJN03_10238, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 354.8 bits (909), Expect = 7.6e-94
Identity = 202/299 (67.56%), Postives = 231/299 (77.26%), Query Frame = 0

Query: 1   MANLPRFGRTWQRFSSLPRPATASQPEQLQPAPAPAPMNGREISPNSPVTAQVLQTSPIR 60
           MANLPRFGRTW RFSSLPRP TA + +     P PA  +  E+ P++P TA VLQTSPI+
Sbjct: 1   MANLPRFGRTWNRFSSLPRPGTAPRLD----VPPPAATSEPEVYPSAPRTANVLQTSPIK 60

Query: 61  ERSSRLPSPTKKFASPPSSPKYRPAA-AASPPKPLSPTPSYNRYDGERRSSATTSPKAIK 120
           ERS R+ SP +K+ SPP+SPKYR AA + SP KPLSP P+YNRYDGERRSSA  SPK  K
Sbjct: 61  ERSPRITSPVRKYPSPPTSPKYRAAAPSTSPRKPLSPPPTYNRYDGERRSSALASPKTFK 120

Query: 121 PSYTSPPLSPAKHKYPTAATAAPLSPLTLPRSEAKHEPGPTIRSRSPPEVEQKSIHYPKV 180
            +YTSPP SPAKHKY T+   APLSPL LP  E +HEP P +R RSPPEV+QKS+ Y K 
Sbjct: 121 TTYTSPPRSPAKHKYSTSTVQAPLSPLALPSLEKRHEPEPMVRPRSPPEVQQKSVLYQKT 180

Query: 181 --EKPTKPDHRPSEYNSGKPQYKQ-QQQQSDVITIKGENVGAIMHITQSSDGTEMVKKKP 240
             EKP K DHR SEY+SGKPQ KQ  QQQSDVI IKGENVGA+MHITQSSD TE  KKKP
Sbjct: 181 TSEKPAKTDHRASEYSSGKPQQKQTHQQQSDVINIKGENVGAVMHITQSSDATETHKKKP 240

Query: 241 STESGNDDEK-GNKSSS-LPAKSFMNSNFQGVNNSVLYNSSFSHRDPGLHLSFSKKPAH 294
           +    +++EK  NKSSS +P KSFMNSNFQGVNNS+LYNSS SHRDPGLHL+F+KKP H
Sbjct: 241 TVSYSSENEKDSNKSSSNMPGKSFMNSNFQGVNNSILYNSSLSHRDPGLHLAFTKKPTH 295

BLAST of Moc05g04890 vs. NCBI nr
Match: XP_023538835.1 (proline-rich extensin-like protein EPR1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 354.0 bits (907), Expect = 1.3e-93
Identity = 203/299 (67.89%), Postives = 230/299 (76.92%), Query Frame = 0

Query: 1   MANLPRFGRTWQRFSSLPRPATASQPEQLQPAPAPAPMNGREISPNSPVTAQVLQTSPIR 60
           MANLPRFGRTW RFSSLPRP TA + +   PA A  P    E+ P  P TA VLQTSPI+
Sbjct: 1   MANLPRFGRTWNRFSSLPRPGTAPRLDVPPPAAASEP----EVYPPPPRTANVLQTSPIK 60

Query: 61  ERSSRLPSPTKKFASPPSSPKYRPAA-AASPPKPLSPTPSYNRYDGERRSSATTSPKAIK 120
           ERS R+PSP +K+ SPP+SPKYR AA + SP KPLSP P+YNRYDGERR+SA  SPK  K
Sbjct: 61  ERSPRIPSPVRKYPSPPTSPKYRAAAPSTSPRKPLSPPPTYNRYDGERRTSALASPKTFK 120

Query: 121 PSYTSPPLSPAKHKYPTAATAAPLSPLTLPRSEAKHEPGPTIRSRSPPEVEQKSIHYPKV 180
            +YTSPP SPAKHKY T+   APLSPL LP  E +HEP P +R RSPPEV+QKS+ Y K 
Sbjct: 121 TTYTSPPRSPAKHKYSTSTLQAPLSPLALPSLEKRHEPEPMVRPRSPPEVQQKSVLYQKT 180

Query: 181 --EKPTKPDHRPSEYNSGKPQYKQ-QQQQSDVITIKGENVGAIMHITQSSDGTEMVKKKP 240
             EKP K DHR SEY+SGKPQ KQ  QQQSDVI IKGENVGA+MHITQSSD TE  KKKP
Sbjct: 181 TSEKPAKTDHRASEYSSGKPQQKQTHQQQSDVINIKGENVGAVMHITQSSDATETHKKKP 240

Query: 241 STESGNDDEK-GNKSSS-LPAKSFMNSNFQGVNNSVLYNSSFSHRDPGLHLSFSKKPAH 294
           +    +++EK  NKSSS +P KSFMNSNFQGVNNS+LYNSS SHRDPGLHL+F+KKP H
Sbjct: 241 TVSYSSENEKDSNKSSSNMPGKSFMNSNFQGVNNSILYNSSLSHRDPGLHLAFTKKPTH 295

BLAST of Moc05g04890 vs. NCBI nr
Match: XP_022974814.1 (DNA-directed RNA polymerase II subunit 1-like isoform X2 [Cucurbita maxima] >XP_022974815.1 DNA-directed RNA polymerase II subunit 1-like isoform X3 [Cucurbita maxima])

HSP 1 Score: 353.2 bits (905), Expect = 2.2e-93
Identity = 202/299 (67.56%), Postives = 231/299 (77.26%), Query Frame = 0

Query: 1   MANLPRFGRTWQRFSSLPRPATASQPEQLQPAPAPAPMNGREISPNSPVTAQVLQTSPIR 60
           MANLPRFGRTW RFSSLPRPATA + +     P PA  +  E+  ++P TA VLQTSPI+
Sbjct: 1   MANLPRFGRTWNRFSSLPRPATAPRFD----VPPPAATSEPEVFSSAPPTANVLQTSPIK 60

Query: 61  ERSSRLPSPTKKFASPPSSPKYRPAA-AASPPKPLSPTPSYNRYDGERRSSATTSPKAIK 120
           ERS R+ SP +K+ SPP+SPKYR AA + SP KPLSP P+YNRYDGERRSSA  SPK  K
Sbjct: 61  ERSPRITSPVRKYPSPPTSPKYRAAAPSTSPRKPLSPPPTYNRYDGERRSSAQASPKTFK 120

Query: 121 PSYTSPPLSPAKHKYPTAATAAPLSPLTLPRSEAKHEPGPTIRSRSPPEVEQKSIHYPKV 180
            +YTSPP SPAKHKY T+   APLSPL LP  E +HEP P +R RSPPEV+QKS+ Y K 
Sbjct: 121 TTYTSPPRSPAKHKYSTSTVQAPLSPLALPSLEKRHEPEPMVRPRSPPEVQQKSVLYQKT 180

Query: 181 --EKPTKPDHRPSEYNSGKPQYKQ-QQQQSDVITIKGENVGAIMHITQSSDGTEMVKKKP 240
             EKP K DHR SEY+SGKPQ KQ  QQQSDVI IKGENVGA+MHITQSSD TE  KKKP
Sbjct: 181 TSEKPAKTDHRASEYSSGKPQQKQTHQQQSDVINIKGENVGAVMHITQSSDATETHKKKP 240

Query: 241 STESGNDDEK-GNKSSS-LPAKSFMNSNFQGVNNSVLYNSSFSHRDPGLHLSFSKKPAH 294
           +    +++EK  NKSSS +P KSFMNSNFQGVNNS+LYNSS SHRDPGLHL+F+KKP H
Sbjct: 241 TVSYSSENEKDSNKSSSNMPGKSFMNSNFQGVNNSILYNSSLSHRDPGLHLAFTKKPTH 295

BLAST of Moc05g04890 vs. ExPASy TrEMBL
Match: A0A6J1D2D2 (proline-rich receptor-like protein kinase PERK2 OS=Momordica charantia OX=3673 GN=LOC111016367 PE=4 SV=1)

HSP 1 Score: 581.6 bits (1498), Expect = 1.9e-162
Identity = 304/304 (100.00%), Postives = 304/304 (100.00%), Query Frame = 0

Query: 1   MANLPRFGRTWQRFSSLPRPATASQPEQLQPAPAPAPMNGREISPNSPVTAQVLQTSPIR 60
           MANLPRFGRTWQRFSSLPRPATASQPEQLQPAPAPAPMNGREISPNSPVTAQVLQTSPIR
Sbjct: 1   MANLPRFGRTWQRFSSLPRPATASQPEQLQPAPAPAPMNGREISPNSPVTAQVLQTSPIR 60

Query: 61  ERSSRLPSPTKKFASPPSSPKYRPAAAASPPKPLSPTPSYNRYDGERRSSATTSPKAIKP 120
           ERSSRLPSPTKKFASPPSSPKYRPAAAASPPKPLSPTPSYNRYDGERRSSATTSPKAIKP
Sbjct: 61  ERSSRLPSPTKKFASPPSSPKYRPAAAASPPKPLSPTPSYNRYDGERRSSATTSPKAIKP 120

Query: 121 SYTSPPLSPAKHKYPTAATAAPLSPLTLPRSEAKHEPGPTIRSRSPPEVEQKSIHYPKVE 180
           SYTSPPLSPAKHKYPTAATAAPLSPLTLPRSEAKHEPGPTIRSRSPPEVEQKSIHYPKVE
Sbjct: 121 SYTSPPLSPAKHKYPTAATAAPLSPLTLPRSEAKHEPGPTIRSRSPPEVEQKSIHYPKVE 180

Query: 181 KPTKPDHRPSEYNSGKPQYKQQQQQSDVITIKGENVGAIMHITQSSDGTEMVKKKPSTES 240
           KPTKPDHRPSEYNSGKPQYKQQQQQSDVITIKGENVGAIMHITQSSDGTEMVKKKPSTES
Sbjct: 181 KPTKPDHRPSEYNSGKPQYKQQQQQSDVITIKGENVGAIMHITQSSDGTEMVKKKPSTES 240

Query: 241 GNDDEKGNKSSSLPAKSFMNSNFQGVNNSVLYNSSFSHRDPGLHLSFSKKPAHGHAQSVL 300
           GNDDEKGNKSSSLPAKSFMNSNFQGVNNSVLYNSSFSHRDPGLHLSFSKKPAHGHAQSVL
Sbjct: 241 GNDDEKGNKSSSLPAKSFMNSNFQGVNNSVLYNSSFSHRDPGLHLSFSKKPAHGHAQSVL 300

Query: 301 GSTY 305
           GSTY
Sbjct: 301 GSTY 304

BLAST of Moc05g04890 vs. ExPASy TrEMBL
Match: A0A6J1G1L2 (DNA-directed RNA polymerase II subunit 1-like OS=Cucurbita moschata OX=3662 GN=LOC111449836 PE=4 SV=1)

HSP 1 Score: 355.1 bits (910), Expect = 2.8e-94
Identity = 202/299 (67.56%), Postives = 231/299 (77.26%), Query Frame = 0

Query: 1   MANLPRFGRTWQRFSSLPRPATASQPEQLQPAPAPAPMNGREISPNSPVTAQVLQTSPIR 60
           MANLPRFGRTW RFSSLPRP TA + +     P PA  +  E+ P++P TA VLQTSPI+
Sbjct: 1   MANLPRFGRTWNRFSSLPRPGTAPRLD----VPPPAATSEPEVYPSAPRTANVLQTSPIK 60

Query: 61  ERSSRLPSPTKKFASPPSSPKYRPAA-AASPPKPLSPTPSYNRYDGERRSSATTSPKAIK 120
           ERS R+ SP +K+ SPP+SPKYR AA + SP KPLSP P+YNRYDGERRSSA  SPK  K
Sbjct: 61  ERSPRITSPVRKYPSPPTSPKYRAAAPSTSPRKPLSPPPTYNRYDGERRSSALASPKTFK 120

Query: 121 PSYTSPPLSPAKHKYPTAATAAPLSPLTLPRSEAKHEPGPTIRSRSPPEVEQKSIHYPKV 180
            +YTSPP SPAKHKY T+   APLSPL LP  E +HEP P +R RSPPEV+QKS+ Y K 
Sbjct: 121 TTYTSPPRSPAKHKYSTSTVQAPLSPLALPSLEKRHEPEPVVRPRSPPEVQQKSVLYQKT 180

Query: 181 --EKPTKPDHRPSEYNSGKPQYKQ-QQQQSDVITIKGENVGAIMHITQSSDGTEMVKKKP 240
             EKP K DHR SEY+SGKPQ KQ  QQQSDVI IKGENVGA+MHITQSSD TE  KKKP
Sbjct: 181 TSEKPAKTDHRASEYSSGKPQQKQTHQQQSDVINIKGENVGAVMHITQSSDATETHKKKP 240

Query: 241 STESGNDDEK-GNKSSS-LPAKSFMNSNFQGVNNSVLYNSSFSHRDPGLHLSFSKKPAH 294
           +    +++EK  NKSSS +P KSFMNSNFQGVNNS+LYNSS SHRDPGLHL+F+KKP H
Sbjct: 241 TVSYSSENEKDSNKSSSNMPGKSFMNSNFQGVNNSILYNSSLSHRDPGLHLAFTKKPTH 295

BLAST of Moc05g04890 vs. ExPASy TrEMBL
Match: A0A6J1IEX0 (DNA-directed RNA polymerase II subunit 1-like isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111473598 PE=4 SV=1)

HSP 1 Score: 353.2 bits (905), Expect = 1.1e-93
Identity = 202/299 (67.56%), Postives = 231/299 (77.26%), Query Frame = 0

Query: 1   MANLPRFGRTWQRFSSLPRPATASQPEQLQPAPAPAPMNGREISPNSPVTAQVLQTSPIR 60
           MANLPRFGRTW RFSSLPRPATA + +     P PA  +  E+  ++P TA VLQTSPI+
Sbjct: 1   MANLPRFGRTWNRFSSLPRPATAPRFD----VPPPAATSEPEVFSSAPPTANVLQTSPIK 60

Query: 61  ERSSRLPSPTKKFASPPSSPKYRPAA-AASPPKPLSPTPSYNRYDGERRSSATTSPKAIK 120
           ERS R+ SP +K+ SPP+SPKYR AA + SP KPLSP P+YNRYDGERRSSA  SPK  K
Sbjct: 61  ERSPRITSPVRKYPSPPTSPKYRAAAPSTSPRKPLSPPPTYNRYDGERRSSAQASPKTFK 120

Query: 121 PSYTSPPLSPAKHKYPTAATAAPLSPLTLPRSEAKHEPGPTIRSRSPPEVEQKSIHYPKV 180
            +YTSPP SPAKHKY T+   APLSPL LP  E +HEP P +R RSPPEV+QKS+ Y K 
Sbjct: 121 TTYTSPPRSPAKHKYSTSTVQAPLSPLALPSLEKRHEPEPMVRPRSPPEVQQKSVLYQKT 180

Query: 181 --EKPTKPDHRPSEYNSGKPQYKQ-QQQQSDVITIKGENVGAIMHITQSSDGTEMVKKKP 240
             EKP K DHR SEY+SGKPQ KQ  QQQSDVI IKGENVGA+MHITQSSD TE  KKKP
Sbjct: 181 TSEKPAKTDHRASEYSSGKPQQKQTHQQQSDVINIKGENVGAVMHITQSSDATETHKKKP 240

Query: 241 STESGNDDEK-GNKSSS-LPAKSFMNSNFQGVNNSVLYNSSFSHRDPGLHLSFSKKPAH 294
           +    +++EK  NKSSS +P KSFMNSNFQGVNNS+LYNSS SHRDPGLHL+F+KKP H
Sbjct: 241 TVSYSSENEKDSNKSSSNMPGKSFMNSNFQGVNNSILYNSSLSHRDPGLHLAFTKKPTH 295

BLAST of Moc05g04890 vs. ExPASy TrEMBL
Match: A0A6J1IHF4 (DNA-directed RNA polymerase II subunit 1-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111473598 PE=4 SV=1)

HSP 1 Score: 352.8 bits (904), Expect = 1.4e-93
Identity = 202/299 (67.56%), Postives = 231/299 (77.26%), Query Frame = 0

Query: 1   MANLPRFGRTWQRFSSLPRPATASQPEQLQPAPAPAPMNGREISPNSPVTAQVLQTSPIR 60
           MANLPRFGRTW RFSSLPRPATA + +     P PA  +  E+  ++P TA VLQTSPI+
Sbjct: 1   MANLPRFGRTWNRFSSLPRPATAPRFD----VPPPAATSEPEVFSSAPPTANVLQTSPIK 60

Query: 61  ERSSRLPSPTKKFASPPSSPKYRPAA-AASPPKPLSPTPSYNRYDGERRSSATTSPKAIK 120
           ERS R+ SP +K+ SPP+SPKYR AA + SP KPLSP P+YNRYDGERRSSA  SPK  K
Sbjct: 61  ERSPRITSPVRKYHSPPTSPKYRAAAPSTSPRKPLSPPPTYNRYDGERRSSAQASPKTFK 120

Query: 121 PSYTSPPLSPAKHKYPTAATAAPLSPLTLPRSEAKHEPGPTIRSRSPPEVEQKSIHYPKV 180
            +YTSPP SPAKHKY T+   APLSPL LP  E +HEP P +R RSPPEV+QKS+ Y K 
Sbjct: 121 TTYTSPPRSPAKHKYSTSTVQAPLSPLALPSLEKRHEPEPMVRPRSPPEVQQKSVLYQKT 180

Query: 181 --EKPTKPDHRPSEYNSGKPQYKQ-QQQQSDVITIKGENVGAIMHITQSSDGTEMVKKKP 240
             EKP K DHR SEY+SGKPQ KQ  QQQSDVI IKGENVGA+MHITQSSD TE  KKKP
Sbjct: 181 TSEKPAKTDHRASEYSSGKPQQKQTHQQQSDVINIKGENVGAVMHITQSSDATETHKKKP 240

Query: 241 STESGNDDEK-GNKSSS-LPAKSFMNSNFQGVNNSVLYNSSFSHRDPGLHLSFSKKPAH 294
           +    +++EK  NKSSS +P KSFMNSNFQGVNNS+LYNSS SHRDPGLHL+F+KKP H
Sbjct: 241 TVSYSSENEKDSNKSSSNMPGKSFMNSNFQGVNNSILYNSSLSHRDPGLHLAFTKKPTH 295

BLAST of Moc05g04890 vs. ExPASy TrEMBL
Match: A0A0A0L5T3 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G119290 PE=4 SV=1)

HSP 1 Score: 316.2 bits (809), Expect = 1.5e-82
Identity = 189/304 (62.17%), Postives = 225/304 (74.01%), Query Frame = 0

Query: 1   MANLPRFGRTWQRFSSLPRPATASQPEQLQPAPAPAPMNGREISPNSPVTAQVLQTSPIR 60
           M+NLPRFGR W RFSSLPRP TA++PE      A  P    E+ P++  T    QTSPI+
Sbjct: 1   MSNLPRFGRQWNRFSSLPRPGTATRPEPQPFTAATEP----EVFPSAVPTTNTFQTSPIK 60

Query: 61  ERSSRLPSPTKKFASPPSSPKYRPAAAASPPKPLSPTPSYNRYDGERRSSATTSPKAIKP 120
           +R+ RL SP KKF+SPPSSPKY  A   SP KPLSP P +NRY+GERR+SATTSPK  KP
Sbjct: 61  QRTPRLSSPVKKFSSPPSSPKYSGAGTVSPRKPLSPPPVHNRYEGERRTSATTSPKTFKP 120

Query: 121 SYTSPPLSPAKHKYPTAATA-APLSPLTLPRSEAKHEPGPTIRSRSPPEVEQKSIHY--P 180
           ++ SPP SP+K ++ T  TA APLSPL LPRS+ + EP  ++R RSPPE+EQK I Y   
Sbjct: 121 THISPPPSPSKPRHSTVPTAVAPLSPLALPRSQVRREPEHSLRPRSPPEIEQKKILYQTT 180

Query: 181 KVEKPTKPDH--RPSEYNSGKPQYKQQQQ--QSDVITIKGENVGAIMHITQSSDGTEMVK 240
             EKPTK DH  +  EY + KPQ KQQ Q  QSDVI IKGENVGA+MHITQSSDG+E++K
Sbjct: 181 TTEKPTKTDHYRQNDEYGASKPQQKQQHQQLQSDVINIKGENVGAVMHITQSSDGSEVIK 240

Query: 241 KKPST-ESGNDDEKGNKS-SSLPAKSFMNSNFQGVNNSVLYNSSFSHRDPGLHLSF-SKK 295
           KKP+  +S  ++EK NKS S+ P KSFMNSNFQGVNNS+LYNSS SHRDPGLHL+F SKK
Sbjct: 241 KKPTVGQSKENEEKTNKSNSNYPGKSFMNSNFQGVNNSILYNSSLSHRDPGLHLAFGSKK 300

BLAST of Moc05g04890 vs. TAIR 10
Match: AT2G46630.1 (unknown protein; LOCATED IN: chloroplast; EXPRESSED IN: 22 plant structures; EXPRESSED DURING: 13 growth stages; Has 110095 Blast hits to 59224 proteins in 2216 species: Archae - 177; Bacteria - 15429; Metazoa - 38345; Fungi - 18843; Plants - 13341; Viruses - 3084; Other Eukaryotes - 20876 (source: NCBI BLink). )

HSP 1 Score: 68.2 bits (165), Expect = 1.3e-11
Identity = 97/338 (28.70%), Postives = 144/338 (42.60%), Query Frame = 0

Query: 16  SLPRPATASQPEQLQPAPAPAPMNGREISPNSPVTAQVLQTSPIRERSSRLPSPTKKFAS 75
           S PR      P + Q  P+P     ++  P +P   +   TSP +ERS    SP  +  S
Sbjct: 47  SPPRQRQPRSPPRQQDPPSPP---RQQQQPLTPPRQKAPPTSPPQERSP-YHSPPSRHMS 106

Query: 76  PPSSPKYRPAAAASPPKPLS-----PTPSY----------NRYDGERRSSATTSPKAIKP 135
           PP+ PK   AA   PP P S     P+P            N       SS +T+ +++K 
Sbjct: 107 PPTPPK---AATPPPPPPRSSYTSPPSPKEVQEALPPRKPNSPPSPAHSSRSTTSESVKT 166

Query: 136 SYTSPPLSPAKHKYPTAATAAPLS-PLTLPRSEAKHEPGPTIRSRSPPEVEQKSIHYPKV 195
              SP  S    K P+    +P S P +L  SE +      + +    +  + + H    
Sbjct: 167 --RSPSESENHRKAPSPRVLSPYSLPASLLHSERETTQKNILTAEKTSQTHETNHHNQNH 226

Query: 196 EKPTKPDHRPSEYNS--------GKPQYKQQQQQSD----------VITIKGENVGAIMH 255
                 +H  ++ +S        G    K  +Q S           VITI GEN GA+M 
Sbjct: 227 NHDYNQNHNYNQNHSYNQNQNHQGNNPKKMHRQPSSSDSENIMSTRVITIAGENKGAVME 286

Query: 256 ITQSSDGTEM------------------VKKKPSTESGNDDEKGNK----------SSSL 292
           I +S  G +                    + + S+ S +D+ +G K          +S+L
Sbjct: 287 ILRSPQGNKTGGSGTHSSRVSHGTGEKGRRLQSSSSSSSDEGEGKKKTTKNVPNKGNSNL 346

BLAST of Moc05g04890 vs. TAIR 10
Match: AT1G63310.1 (unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT2G20362.1); Has 78 Blast hits to 77 proteins in 11 species: Archae - 0; Bacteria - 0; Metazoa - 0; Fungi - 0; Plants - 78; Viruses - 0; Other Eukaryotes - 0 (source: NCBI BLink). )

HSP 1 Score: 46.6 bits (109), Expect = 4.1e-05
Identity = 30/95 (31.58%), Postives = 48/95 (50.53%), Query Frame = 0

Query: 194 SGKPQYKQQQQQSDVITIKGENVGAIMHITQSSDGTEMVKKKPSTESGNDDEKGNKSSSL 253
           +G  QY +++    VIT+ G N+GA M            K +     G+ D+ G+     
Sbjct: 38  AGPSQYDEEEDGIRVITLSGSNLGATM------------KTELDNNHGDRDQNGDHELDF 97

Query: 254 PAKSFMNSNFQGVNNSVLYNSSFSHRDPGLHLSFS 289
              +++NSNFQ VNNS++  + +   DPG+HL  S
Sbjct: 98  -LSTYVNSNFQAVNNSIMIGAKYETHDPGVHLDIS 119

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022147442.13.8e-162100.00proline-rich receptor-like protein kinase PERK2 [Momordica charantia][more]
XP_022945677.15.8e-9467.56DNA-directed RNA polymerase II subunit 1-like [Cucurbita moschata][more]
KAG6597058.17.6e-9467.56hypothetical protein SDJN03_10238, partial [Cucurbita argyrosperma subsp. sorori... [more]
XP_023538835.11.3e-9367.89proline-rich extensin-like protein EPR1 [Cucurbita pepo subsp. pepo][more]
XP_022974814.12.2e-9367.56DNA-directed RNA polymerase II subunit 1-like isoform X2 [Cucurbita maxima] >XP_... [more]
Match NameE-valueIdentityDescription
Match NameE-valueIdentityDescription
A0A6J1D2D21.9e-162100.00proline-rich receptor-like protein kinase PERK2 OS=Momordica charantia OX=3673 G... [more]
A0A6J1G1L22.8e-9467.56DNA-directed RNA polymerase II subunit 1-like OS=Cucurbita moschata OX=3662 GN=L... [more]
A0A6J1IEX01.1e-9367.56DNA-directed RNA polymerase II subunit 1-like isoform X2 OS=Cucurbita maxima OX=... [more]
A0A6J1IHF41.4e-9367.56DNA-directed RNA polymerase II subunit 1-like isoform X1 OS=Cucurbita maxima OX=... [more]
A0A0A0L5T31.5e-8262.17Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G119290 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
AT2G46630.11.3e-1128.70unknown protein; LOCATED IN: chloroplast; EXPRESSED IN: 22 plant structures; EXP... [more]
AT1G63310.14.1e-0531.58unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TA... [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (OHB3-1) v2
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 43..72
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 232..250
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 194..211
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 102..121
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 168..193
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..262
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 15..29
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 83..97
NoneNo IPR availablePANTHERPTHR33472:SF1EXTENSIN-RELATEDcoord: 200..293
NoneNo IPR availablePANTHERPTHR33472OS01G0106600 PROTEINcoord: 200..293

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Moc05g04890.1Moc05g04890.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0016310 phosphorylation
molecular_function GO:0016301 kinase activity