Moc05g04850 (gene) Bitter gourd (OHB3-1) v2

Overview
NameMoc05g04850
Typegene
OrganismMomordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
DescriptionAAA-ATPase
Locationchr5: 3343504 .. 3344949 (-)
RNA-Seq ExpressionMoc05g04850
SyntenyMoc05g04850
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGAGTTGTTTTTCAGGTTGTTTTGTTTAATCCTCATCGGAATTTGTTTCACTTGGTTGTTGCGGATTGTTCTATTCAGGACGGGGTTGATTTTCCTGGTCAAAAAATGGTGGAGAAATCTACAAGATTGTTTCCATGTTTACCAGTCTTTGAGAATCCCGGAATTCAATGAGAGCTCCCAGAGGAATCTCTTGTATTGGAAAGTCTCTGCCTATCTCAGTTCCTTGTCCTCTCTCGAGGATTCCGATTTCACCAATCTCGTCACCGGGAAGAAGCCGAATGACATCCTTCTCCGATTGGATTCGAATCAGACCGTCGTGGACGAATTTTTGGGGGCCAGATTGCTGTGGACGAATGAAGAAAACGGTAGCAGGAATTTTGTGTTGAAAATTAGAAGGGCTGATAAGCGACGAATCCTCCGCCCCTATCTTCAGCACATTCATGCACTAACCGCGGATGAGATTGAGCACAACAAGGGGGATTTGAGGCTTTTTATGAATTCGAAATTCAACAATCAATCGCATACAAGGTGGAAGTCAATCCAATTCAACCATCCCTCCACCTTTGAATCGATTTCCATGGAGCCAGATCTCAAAGAAAAGGTCAAATCCGATCTCGAATCGTTTCTCAAATCAAAACAATATTACCACAAATTAGGCAGAGTATGGAAACGGAGTTACCTTCTATATGGCCCTTCCGGAACCGGAAAATCGAGCTTTGTTGCCGCCATGGCGAATTTCCTCTCCTACGACGTTTACGACATCGACCTATTCAAAGTCTCCGATGAATCTGATCTCAAGCTTCTTCTGCTGCAGACCACCAGCAAGTCGATAATCGTCGTCGAGGATCTCGATCGGTTTCTGATCGAGAAGTCGCCGGCGTTGAGTCTATCGGCGTTGCTGAACTTCATGGACGGAATTTTGACATCGTGCTGCGCGGAGGAGAGGATGATGGTTTTCACCGTGAATTGCAAGGAACAGGTCGACCCGGCGATTCTCCGGCCGGGCCGTATCGACGTTCACATCCATTTTCCGCTCTGCGATTTCTCCGCTTTCAAGAATCTCGCAATCAACTACCTTGGCGTGAAGGATCACAAATTGTTTCCTCAGGTTGAGGAAATATTCCAAACCGGCGCCAGTTTGAGCCCGGCCGAGATCGGCGAGCTCATGATTGCGAACCGGAGCTCGCCGAGCCGAGCGATTAAATCAGTCATCTCAGCGTTGCAGACCGACGGCGAGCGGCGGAGAGTCGGCAACATGGGGCGGCGGTTGAGCAATTGCGGATCCAGGAAGTCCATCGACGAATCCGTCGAATCGAGCGGCGTCTTCTGCAGCGAAAACGCTCACACCGGAAAAGAGTTCAGGAAATTGTACGGTTTTCTGAGGATGAAAAGCAACAAAATATCTCAGTCGTTCGATCAATCTCCGCGCCACAAAGACGGTTGA

mRNA sequence

ATGGAGTTGTTTTTCAGGTTGTTTTGTTTAATCCTCATCGGAATTTGTTTCACTTGGTTGTTGCGGATTGTTCTATTCAGGACGGGGTTGATTTTCCTGGTCAAAAAATGGTGGAGAAATCTACAAGATTGTTTCCATGTTTACCAGTCTTTGAGAATCCCGGAATTCAATGAGAGCTCCCAGAGGAATCTCTTGTATTGGAAAGTCTCTGCCTATCTCAGTTCCTTGTCCTCTCTCGAGGATTCCGATTTCACCAATCTCGTCACCGGGAAGAAGCCGAATGACATCCTTCTCCGATTGGATTCGAATCAGACCGTCGTGGACGAATTTTTGGGGGCCAGATTGCTGTGGACGAATGAAGAAAACGGTAGCAGGAATTTTGTGTTGAAAATTAGAAGGGCTGATAAGCGACGAATCCTCCGCCCCTATCTTCAGCACATTCATGCACTAACCGCGGATGAGATTGAGCACAACAAGGGGGATTTGAGGCTTTTTATGAATTCGAAATTCAACAATCAATCGCATACAAGGTGGAAGTCAATCCAATTCAACCATCCCTCCACCTTTGAATCGATTTCCATGGAGCCAGATCTCAAAGAAAAGGTCAAATCCGATCTCGAATCGTTTCTCAAATCAAAACAATATTACCACAAATTAGGCAGAGTATGGAAACGGAGTTACCTTCTATATGGCCCTTCCGGAACCGGAAAATCGAGCTTTGTTGCCGCCATGGCGAATTTCCTCTCCTACGACGTTTACGACATCGACCTATTCAAAGTCTCCGATGAATCTGATCTCAAGCTTCTTCTGCTGCAGACCACCAGCAAGTCGATAATCGTCGTCGAGGATCTCGATCGGTTTCTGATCGAGAAGTCGCCGGCGTTGAGTCTATCGGCGTTGCTGAACTTCATGGACGGAATTTTGACATCGTGCTGCGCGGAGGAGAGGATGATGGTTTTCACCGTGAATTGCAAGGAACAGGTCGACCCGGCGATTCTCCGGCCGGGCCGTATCGACGTTCACATCCATTTTCCGCTCTGCGATTTCTCCGCTTTCAAGAATCTCGCAATCAACTACCTTGGCGTGAAGGATCACAAATTGTTTCCTCAGGTTGAGGAAATATTCCAAACCGGCGCCAGTTTGAGCCCGGCCGAGATCGGCGAGCTCATGATTGCGAACCGGAGCTCGCCGAGCCGAGCGATTAAATCAGTCATCTCAGCGTTGCAGACCGACGGCGAGCGGCGGAGAGTCGGCAACATGGGGCGGCGGTTGAGCAATTGCGGATCCAGGAAGTCCATCGACGAATCCGTCGAATCGAGCGGCGTCTTCTGCAGCGAAAACGCTCACACCGGAAAAGAGTTCAGGAAATTGTACGGTTTTCTGAGGATGAAAAGCAACAAAATATCTCAGTCGTTCGATCAATCTCCGCGCCACAAAGACGGTTGA

Coding sequence (CDS)

ATGGAGTTGTTTTTCAGGTTGTTTTGTTTAATCCTCATCGGAATTTGTTTCACTTGGTTGTTGCGGATTGTTCTATTCAGGACGGGGTTGATTTTCCTGGTCAAAAAATGGTGGAGAAATCTACAAGATTGTTTCCATGTTTACCAGTCTTTGAGAATCCCGGAATTCAATGAGAGCTCCCAGAGGAATCTCTTGTATTGGAAAGTCTCTGCCTATCTCAGTTCCTTGTCCTCTCTCGAGGATTCCGATTTCACCAATCTCGTCACCGGGAAGAAGCCGAATGACATCCTTCTCCGATTGGATTCGAATCAGACCGTCGTGGACGAATTTTTGGGGGCCAGATTGCTGTGGACGAATGAAGAAAACGGTAGCAGGAATTTTGTGTTGAAAATTAGAAGGGCTGATAAGCGACGAATCCTCCGCCCCTATCTTCAGCACATTCATGCACTAACCGCGGATGAGATTGAGCACAACAAGGGGGATTTGAGGCTTTTTATGAATTCGAAATTCAACAATCAATCGCATACAAGGTGGAAGTCAATCCAATTCAACCATCCCTCCACCTTTGAATCGATTTCCATGGAGCCAGATCTCAAAGAAAAGGTCAAATCCGATCTCGAATCGTTTCTCAAATCAAAACAATATTACCACAAATTAGGCAGAGTATGGAAACGGAGTTACCTTCTATATGGCCCTTCCGGAACCGGAAAATCGAGCTTTGTTGCCGCCATGGCGAATTTCCTCTCCTACGACGTTTACGACATCGACCTATTCAAAGTCTCCGATGAATCTGATCTCAAGCTTCTTCTGCTGCAGACCACCAGCAAGTCGATAATCGTCGTCGAGGATCTCGATCGGTTTCTGATCGAGAAGTCGCCGGCGTTGAGTCTATCGGCGTTGCTGAACTTCATGGACGGAATTTTGACATCGTGCTGCGCGGAGGAGAGGATGATGGTTTTCACCGTGAATTGCAAGGAACAGGTCGACCCGGCGATTCTCCGGCCGGGCCGTATCGACGTTCACATCCATTTTCCGCTCTGCGATTTCTCCGCTTTCAAGAATCTCGCAATCAACTACCTTGGCGTGAAGGATCACAAATTGTTTCCTCAGGTTGAGGAAATATTCCAAACCGGCGCCAGTTTGAGCCCGGCCGAGATCGGCGAGCTCATGATTGCGAACCGGAGCTCGCCGAGCCGAGCGATTAAATCAGTCATCTCAGCGTTGCAGACCGACGGCGAGCGGCGGAGAGTCGGCAACATGGGGCGGCGGTTGAGCAATTGCGGATCCAGGAAGTCCATCGACGAATCCGTCGAATCGAGCGGCGTCTTCTGCAGCGAAAACGCTCACACCGGAAAAGAGTTCAGGAAATTGTACGGTTTTCTGAGGATGAAAAGCAACAAAATATCTCAGTCGTTCGATCAATCTCCGCGCCACAAAGACGGTTGA

Protein sequence

MELFFRLFCLILIGICFTWLLRIVLFRTGLIFLVKKWWRNLQDCFHVYQSLRIPEFNESSQRNLLYWKVSAYLSSLSSLEDSDFTNLVTGKKPNDILLRLDSNQTVVDEFLGARLLWTNEENGSRNFVLKIRRADKRRILRPYLQHIHALTADEIEHNKGDLRLFMNSKFNNQSHTRWKSIQFNHPSTFESISMEPDLKEKVKSDLESFLKSKQYYHKLGRVWKRSYLLYGPSGTGKSSFVAAMANFLSYDVYDIDLFKVSDESDLKLLLLQTTSKSIIVVEDLDRFLIEKSPALSLSALLNFMDGILTSCCAEERMMVFTVNCKEQVDPAILRPGRIDVHIHFPLCDFSAFKNLAINYLGVKDHKLFPQVEEIFQTGASLSPAEIGELMIANRSSPSRAIKSVISALQTDGERRRVGNMGRRLSNCGSRKSIDESVESSGVFCSENAHTGKEFRKLYGFLRMKSNKISQSFDQSPRHKDG
Homology
BLAST of Moc05g04850 vs. NCBI nr
Match: XP_038903225.1 (AAA-ATPase At2g46620 [Benincasa hispida])

HSP 1 Score: 838.6 bits (2165), Expect = 2.8e-239
Identity = 423/480 (88.12%), Postives = 451/480 (93.96%), Query Frame = 0

Query: 1   MELFFRLFCLILIGICFTWLLRIVLFRTGLIFLVKKWWRNLQDCFHVYQSLRIPEFNESS 60
           MELFFRLF +I IGICF WLLR +LFRTGLIFLVKKWW NL+DCFHVYQS RIPEFNE+S
Sbjct: 1   MELFFRLFSVIFIGICFCWLLRNLLFRTGLIFLVKKWWGNLEDCFHVYQSFRIPEFNETS 60

Query: 61  QRNLLYWKVSAYLSSLSSLEDSDFTNLVTGKKPNDILLRLDSNQTVVDEFLGARLLWTNE 120
           Q+N LY KVSAYL+SLSSLEDSDFTNL+TG KPNDI+LRLDSNQTV D FLGA++ WTNE
Sbjct: 61  QQNHLYRKVSAYLTSLSSLEDSDFTNLITGNKPNDIILRLDSNQTVQDNFLGAKVFWTNE 120

Query: 121 ENGSRNFVLKIRRADKRRILRPYLQHIHALTADEIEHNKGDLRLFMNSKFNNQSHTRWKS 180
           +NGSRNFVLKIR+ADKRRILRPYLQHIH LTADEIE  KGDL+LFMNSK NNQS TRWKS
Sbjct: 121 QNGSRNFVLKIRKADKRRILRPYLQHIHTLTADEIEQRKGDLKLFMNSKPNNQSDTRWKS 180

Query: 181 IQFNHPSTFESISMEPDLKEKVKSDLESFLKSKQYYHKLGRVWKRSYLLYGPSGTGKSSF 240
           IQF HPSTF+SI+ME DLKE+VKSDLESFLKSKQYYH+LGRVWKRSYLLYGPSGTGKSSF
Sbjct: 181 IQFKHPSTFDSIAMETDLKERVKSDLESFLKSKQYYHRLGRVWKRSYLLYGPSGTGKSSF 240

Query: 241 VAAMANFLSYDVYDIDLFKVSDESDLKLLLLQTTSKSIIVVEDLDRFLIEKSPALSLSAL 300
           VAAMANFLSYDVYDIDLFKVSD+SDLK LLLQTTSKSIIVVEDLDRFLIEKS ALSLSAL
Sbjct: 241 VAAMANFLSYDVYDIDLFKVSDDSDLKSLLLQTTSKSIIVVEDLDRFLIEKSSALSLSAL 300

Query: 301 LNFMDGILTSCCAEERMMVFTVNCKEQVDPAILRPGRIDVHIHFPLCDFSAFKNLAINYL 360
           LNFMDGILTSCCAEER+MVFTVNCKEQV+PAILRPGRIDVHIHFPLCDFSAFKNLAINYL
Sbjct: 301 LNFMDGILTSCCAEERVMVFTVNCKEQVEPAILRPGRIDVHIHFPLCDFSAFKNLAINYL 360

Query: 361 GVKDHKLFPQVEEIFQTGASLSPAEIGELMIANRSSPSRAIKSVISALQTDGERRRVGNM 420
           GVKDHKLFPQVEEIFQ GASLSPAEIGELMIANR+SPSRAIKSVISALQTDG+RRRV N+
Sbjct: 361 GVKDHKLFPQVEEIFQNGASLSPAEIGELMIANRNSPSRAIKSVISALQTDGDRRRVANI 420

Query: 421 GRRLSNCGSRKSIDESVESSGVFCSENAHTGKEFRKLYGFLRMKSNKISQSFDQSPRHKD 480
           GRRLS+CGSRKS+ ESVES GVFC+EN  TGKEF+KLYGFLRMKSNKISQSFD SP HK+
Sbjct: 421 GRRLSDCGSRKSVAESVESGGVFCNENTQTGKEFKKLYGFLRMKSNKISQSFDSSPLHKE 480

BLAST of Moc05g04850 vs. NCBI nr
Match: XP_008437854.1 (PREDICTED: AAA-ATPase At2g46620 [Cucumis melo])

HSP 1 Score: 831.2 bits (2146), Expect = 4.4e-237
Identity = 418/480 (87.08%), Postives = 447/480 (93.12%), Query Frame = 0

Query: 1   MELFFRLFCLILIGICFTWLLRIVLFRTGLIFLVKKWWRNLQDCFHVYQSLRIPEFNESS 60
           MELFFR F +I IGICF+WLLRI+LFRTGLIF+VKKWW NL+DCFHVYQS RIPEFNE+S
Sbjct: 1   MELFFRFFYVIFIGICFSWLLRILLFRTGLIFIVKKWWANLEDCFHVYQSFRIPEFNETS 60

Query: 61  QRNLLYWKVSAYLSSLSSLEDSDFTNLVTGKKPNDILLRLDSNQTVVDEFLGARLLWTNE 120
           Q N LY KVSAYL+SLSSLEDSD+TNL+TG KPNDI+LRLDSNQTV D FLGA++ WTNE
Sbjct: 61  QHNHLYRKVSAYLTSLSSLEDSDYTNLITGNKPNDIILRLDSNQTVQDNFLGAKVFWTNE 120

Query: 121 ENGSRNFVLKIRRADKRRILRPYLQHIHALTADEIEHNKGDLRLFMNSKFNNQSHTRWKS 180
           +NGSRNFVLKIR+ADKRRILRPYLQHIH LTAD+ E  KGDL+LFMNSK NN S TRWKS
Sbjct: 121 QNGSRNFVLKIRKADKRRILRPYLQHIHTLTADDNEQRKGDLKLFMNSKPNNHSDTRWKS 180

Query: 181 IQFNHPSTFESISMEPDLKEKVKSDLESFLKSKQYYHKLGRVWKRSYLLYGPSGTGKSSF 240
           IQF HPSTF+SI+ME DLKEKVKSDLESFLKSKQYYH+LGRVWKRSYLLYGPSGTGKSSF
Sbjct: 181 IQFKHPSTFDSIAMETDLKEKVKSDLESFLKSKQYYHRLGRVWKRSYLLYGPSGTGKSSF 240

Query: 241 VAAMANFLSYDVYDIDLFKVSDESDLKLLLLQTTSKSIIVVEDLDRFLIEKSPALSLSAL 300
           VAAMANFLSYDVYDIDLFKVSD+SDLK LLLQTTSKS+IVVEDLDRFLIEKS ALSLSAL
Sbjct: 241 VAAMANFLSYDVYDIDLFKVSDDSDLKFLLLQTTSKSVIVVEDLDRFLIEKSSALSLSAL 300

Query: 301 LNFMDGILTSCCAEERMMVFTVNCKEQVDPAILRPGRIDVHIHFPLCDFSAFKNLAINYL 360
           LNFMDGILTSCCAEER+MVFTVNCKEQV+PAILRPGRIDVHIHFPLCDFSAFKNLAINYL
Sbjct: 301 LNFMDGILTSCCAEERVMVFTVNCKEQVEPAILRPGRIDVHIHFPLCDFSAFKNLAINYL 360

Query: 361 GVKDHKLFPQVEEIFQTGASLSPAEIGELMIANRSSPSRAIKSVISALQTDGERRRVGNM 420
           GVKDHKLFPQVEEIFQTGASLSPAEI ELMIANR+SPSRAIKSVISALQTDG+RRRV N+
Sbjct: 361 GVKDHKLFPQVEEIFQTGASLSPAEISELMIANRNSPSRAIKSVISALQTDGDRRRVSNI 420

Query: 421 GRRLSNCGSRKSIDESVESSGVFCSENAHTGKEFRKLYGFLRMKSNKISQSFDQSPRHKD 480
           GRRLS+CGSRKS+ ES+ES GV CSEN  TGKEFRKLYGFLRMKSNKISQSFD SP  KD
Sbjct: 421 GRRLSDCGSRKSVAESIESGGVLCSENTQTGKEFRKLYGFLRMKSNKISQSFDSSPIRKD 480

BLAST of Moc05g04850 vs. NCBI nr
Match: XP_004133798.1 (AAA-ATPase At2g46620 [Cucumis sativus])

HSP 1 Score: 828.6 bits (2139), Expect = 2.9e-236
Identity = 418/480 (87.08%), Postives = 445/480 (92.71%), Query Frame = 0

Query: 1   MELFFRLFCLILIGICFTWLLRIVLFRTGLIFLVKKWWRNLQDCFHVYQSLRIPEFNESS 60
           MELFFR F  I IGICF+WLLRI+LFRTGLIFLVKKWW NL+DCFHVYQS RIPEFNE+S
Sbjct: 1   MELFFRFFSAIFIGICFSWLLRILLFRTGLIFLVKKWWANLEDCFHVYQSFRIPEFNETS 60

Query: 61  QRNLLYWKVSAYLSSLSSLEDSDFTNLVTGKKPNDILLRLDSNQTVVDEFLGARLLWTNE 120
           Q N LY KVSAYL+SLSSLEDSDFTNL+TG KPNDI+LRLDSNQTV D FLGA++ WTNE
Sbjct: 61  QHNHLYRKVSAYLTSLSSLEDSDFTNLITGNKPNDIILRLDSNQTVQDNFLGAKVFWTNE 120

Query: 121 ENGSRNFVLKIRRADKRRILRPYLQHIHALTADEIEHNKGDLRLFMNSKFNNQSHTRWKS 180
           + GSRNFVL+IR+ADKRRILRPYLQHIH LTADE E  KGDL+LFMNSK NN S TRWKS
Sbjct: 121 QKGSRNFVLRIRKADKRRILRPYLQHIHTLTADENEQRKGDLKLFMNSKPNNHSDTRWKS 180

Query: 181 IQFNHPSTFESISMEPDLKEKVKSDLESFLKSKQYYHKLGRVWKRSYLLYGPSGTGKSSF 240
           IQF HPSTF+SI+ME DLKEKVKSDLESFLKSKQYYH+LGRVWKRSYLLYGPSGTGKSSF
Sbjct: 181 IQFKHPSTFDSIAMETDLKEKVKSDLESFLKSKQYYHRLGRVWKRSYLLYGPSGTGKSSF 240

Query: 241 VAAMANFLSYDVYDIDLFKVSDESDLKLLLLQTTSKSIIVVEDLDRFLIEKSPALSLSAL 300
           VAAMANFLSYDVYDIDLFKVSD+SDLK LLLQTTSKS+IVVEDLDRFLIEKS ALSLSAL
Sbjct: 241 VAAMANFLSYDVYDIDLFKVSDDSDLKFLLLQTTSKSVIVVEDLDRFLIEKSSALSLSAL 300

Query: 301 LNFMDGILTSCCAEERMMVFTVNCKEQVDPAILRPGRIDVHIHFPLCDFSAFKNLAINYL 360
           LNFMDGILTSCCAEER+MVFTVNCKEQV+PAILRPGRIDVHIHFPLCDFSAFKNLAINYL
Sbjct: 301 LNFMDGILTSCCAEERVMVFTVNCKEQVEPAILRPGRIDVHIHFPLCDFSAFKNLAINYL 360

Query: 361 GVKDHKLFPQVEEIFQTGASLSPAEIGELMIANRSSPSRAIKSVISALQTDGERRRVGNM 420
           GVKDHKLFPQVEEIFQTGASLSPAEI ELMIANR+SPSRAIKSVISALQTDG+RRRV N+
Sbjct: 361 GVKDHKLFPQVEEIFQTGASLSPAEISELMIANRNSPSRAIKSVISALQTDGDRRRVSNI 420

Query: 421 GRRLSNCGSRKSIDESVESSGVFCSENAHTGKEFRKLYGFLRMKSNKISQSFDQSPRHKD 480
           GRRLS+CGSRKS+ ES+ES GV CSEN  TGKEFRKLYGFLRMKSNKISQSFD SP  K+
Sbjct: 421 GRRLSDCGSRKSVAESIESGGVVCSENTQTGKEFRKLYGFLRMKSNKISQSFDSSPIRKE 480

BLAST of Moc05g04850 vs. NCBI nr
Match: XP_023527367.1 (AAA-ATPase At2g46620-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 815.8 bits (2106), Expect = 1.9e-232
Identity = 411/480 (85.62%), Postives = 443/480 (92.29%), Query Frame = 0

Query: 1   MELFFRLFCLILIGICFTWLLRIVLFRTGLIFLVKKWWRNLQDCFHVYQSLRIPEFNESS 60
           MELFFRLF +I IGICF WLLRIVLFRTG +F+VKKWWRNL+DCFHVYQS RIPEFN+SS
Sbjct: 1   MELFFRLFFVIFIGICFCWLLRIVLFRTGFVFVVKKWWRNLEDCFHVYQSFRIPEFNQSS 60

Query: 61  QRNLLYWKVSAYLSSLSSLEDSDFTNLVTGKKPNDILLRLDSNQTVVDEFLGARLLWTNE 120
           Q+N LY KVSAYL+SLSSLEDSDFTNL+TG  PNDI+LRLDSNQTV D FLGA+LLWTNE
Sbjct: 61  QQNHLYRKVSAYLTSLSSLEDSDFTNLITGNAPNDIILRLDSNQTVQDNFLGAKLLWTNE 120

Query: 121 ENGSRNFVLKIRRADKRRILRPYLQHIHALTADEIEHNKGDLRLFMNSKFNNQSHTRWKS 180
           ENGSRNFVL+IR+ADKRRILRPYLQHIH LTAD IE  KGDL+LFMNSK NNQS TRWKS
Sbjct: 121 ENGSRNFVLRIRKADKRRILRPYLQHIHTLTADVIEQRKGDLKLFMNSKPNNQSDTRWKS 180

Query: 181 IQFNHPSTFESISMEPDLKEKVKSDLESFLKSKQYYHKLGRVWKRSYLLYGPSGTGKSSF 240
           IQF HPSTF+SISME DLKE+VKSDLESFLKSKQYYH+LGRVWKRSYLLYGPSGTGKSSF
Sbjct: 181 IQFKHPSTFDSISMETDLKERVKSDLESFLKSKQYYHRLGRVWKRSYLLYGPSGTGKSSF 240

Query: 241 VAAMANFLSYDVYDIDLFKVSDESDLKLLLLQTTSKSIIVVEDLDRFLIEKSPALSLSAL 300
           VAAMANFLSY+VYDIDLFKVSD+SDLKLLLLQTT+KSIIVVEDLDRFL++KS  LSLSAL
Sbjct: 241 VAAMANFLSYNVYDIDLFKVSDDSDLKLLLLQTTTKSIIVVEDLDRFLMDKSSTLSLSAL 300

Query: 301 LNFMDGILTSCCAEERMMVFTVNCKEQVDPAILRPGRIDVHIHFPLCDFSAFKNLAINYL 360
           LNFMDGILTSCCAEER+MVFTVNCK+ VDPA+LRPGRIDVHIHFPLCDFSAFKNLA NYL
Sbjct: 301 LNFMDGILTSCCAEERVMVFTVNCKDLVDPAVLRPGRIDVHIHFPLCDFSAFKNLANNYL 360

Query: 361 GVKDHKLFPQVEEIFQTGASLSPAEIGELMIANRSSPSRAIKSVISALQTDGERRRVGNM 420
           GVKDHKLFPQVEEIFQ GASLSPAEI ELMI NR+SPSRAIKSVI+ALQTD ERRR+ ++
Sbjct: 361 GVKDHKLFPQVEEIFQNGASLSPAEISELMITNRNSPSRAIKSVITALQTDVERRRISSI 420

Query: 421 GRRLSNCGSRKSIDESVESSGVFCSENAHTGKEFRKLYGFLRMKSNKISQSFDQSPRHKD 480
           GRRLS+CGSRKS+ ESVES GV CSEN  TGKE RKLYGFLRMKSNKISQ+FD SP  KD
Sbjct: 421 GRRLSDCGSRKSVAESVESGGVLCSENTQTGKELRKLYGFLRMKSNKISQTFDTSPMRKD 480

BLAST of Moc05g04850 vs. NCBI nr
Match: XP_022924392.1 (AAA-ATPase At2g46620-like [Cucurbita moschata] >KAG6582688.1 AAA-ATPase, partial [Cucurbita argyrosperma subsp. sororia] >KAG7019085.1 AAA-ATPase, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 808.1 bits (2086), Expect = 4.0e-230
Identity = 409/480 (85.21%), Postives = 441/480 (91.88%), Query Frame = 0

Query: 1   MELFFRLFCLILIGICFTWLLRIVLFRTGLIFLVKKWWRNLQDCFHVYQSLRIPEFNESS 60
           MELFFR F +I IGICF WLLRIVLFRTG +F+VKK WRNL+DCFHVYQS RIPEFN+SS
Sbjct: 1   MELFFRSFFVIFIGICFCWLLRIVLFRTGFVFVVKKRWRNLEDCFHVYQSFRIPEFNQSS 60

Query: 61  QRNLLYWKVSAYLSSLSSLEDSDFTNLVTGKKPNDILLRLDSNQTVVDEFLGARLLWTNE 120
           Q+N LY KVSAYL+SLSSLEDSDFTNL+TG  PNDI+LRLDSNQTV D FLGA+LLWTNE
Sbjct: 61  QQNHLYRKVSAYLTSLSSLEDSDFTNLITGNAPNDIILRLDSNQTVQDNFLGAKLLWTNE 120

Query: 121 ENGSRNFVLKIRRADKRRILRPYLQHIHALTADEIEHNKGDLRLFMNSKFNNQSHTRWKS 180
           ENGSRNFVL+IR+ADKRRILRPYLQHIH LTAD IE  KGDL+LFMNSK NNQS TRWKS
Sbjct: 121 ENGSRNFVLRIRKADKRRILRPYLQHIHTLTADVIEQRKGDLKLFMNSKPNNQSDTRWKS 180

Query: 181 IQFNHPSTFESISMEPDLKEKVKSDLESFLKSKQYYHKLGRVWKRSYLLYGPSGTGKSSF 240
           IQF HPSTF+SISME DLKE+VKSDLESFLKSKQYYH+LGRVWKRSYLLYGPSGTGKSSF
Sbjct: 181 IQFKHPSTFDSISMETDLKERVKSDLESFLKSKQYYHRLGRVWKRSYLLYGPSGTGKSSF 240

Query: 241 VAAMANFLSYDVYDIDLFKVSDESDLKLLLLQTTSKSIIVVEDLDRFLIEKSPALSLSAL 300
           VAAMANFLSY+VYDIDLFKVSD+SDLKLLLLQTT+KSIIVVEDLDRFL++KS  LSLSAL
Sbjct: 241 VAAMANFLSYNVYDIDLFKVSDDSDLKLLLLQTTTKSIIVVEDLDRFLMDKSSTLSLSAL 300

Query: 301 LNFMDGILTSCCAEERMMVFTVNCKEQVDPAILRPGRIDVHIHFPLCDFSAFKNLAINYL 360
           LNFMDGILTSCCAEER+MVFTVNCK+ VDPA+LRPGRIDVHIHFPLCDFSAFKNLA NYL
Sbjct: 301 LNFMDGILTSCCAEERVMVFTVNCKDLVDPAVLRPGRIDVHIHFPLCDFSAFKNLANNYL 360

Query: 361 GVKDHKLFPQVEEIFQTGASLSPAEIGELMIANRSSPSRAIKSVISALQTDGERRRVGNM 420
           GVKDHKLFPQVEEIFQ GASLSPAEI ELMI NR+SPSRAIKSVI+ALQTD ERRR+ ++
Sbjct: 361 GVKDHKLFPQVEEIFQNGASLSPAEISELMITNRNSPSRAIKSVITALQTDVERRRISSI 420

Query: 421 GRRLSNCGSRKSIDESVESSGVFCSENAHTGKEFRKLYGFLRMKSNKISQSFDQSPRHKD 480
           GRRLS+CGSRKS+ ESVES GV CSEN  TGKE RKLYGFLRMKSNKISQ+FD SP  KD
Sbjct: 421 GRRLSDCGSRKSVAESVESGGVLCSENTQTGKELRKLYGFLRMKSNKISQTFDTSPMRKD 480

BLAST of Moc05g04850 vs. ExPASy Swiss-Prot
Match: F4IJ77 (AAA-ATPase At2g46620 OS=Arabidopsis thaliana OX=3702 GN=At2g46620 PE=2 SV=1)

HSP 1 Score: 583.6 bits (1503), Expect = 2.1e-165
Identity = 314/494 (63.56%), Postives = 384/494 (77.73%), Query Frame = 0

Query: 1   MELFFRLFCLILIGICFTWLLRIVLFRTGLIFLVKKWWRNLQDCFHVYQSLRIPEFNESS 60
           M + +  F L+L+     +L+RI+LF+TGLI++VK W R + D FHVYQ  ++PEFN++ 
Sbjct: 1   MGILWDSFLLLLVSTFALFLVRILLFKTGLIYMVKLWRRKIIDWFHVYQFYKVPEFNDNV 60

Query: 61  QRNLLYWKVSAYLSSLSSLEDSDFTNLVTGKKPNDILLRLDSNQTVVDEFLGARLLWTN- 120
           Q N LY KV  YL+SLSS+E+SDFTNL TGKK N+I+LRLD NQ V DEFLGAR+ W N 
Sbjct: 61  QENHLYQKVYMYLNSLSSIENSDFTNLFTGKKSNEIILRLDRNQVVGDEFLGARVCWING 120

Query: 121 -EENGSRNFVLKIRRADKRRILRPYLQHIHALTADEIEHNKGDLRLFMNSKFNN------ 180
            +E+G+RNFVLKIR+ADKRRIL  YLQHIH + +DE+E    +L+LF+N   ++      
Sbjct: 121 EDEDGARNFVLKIRKADKRRILGSYLQHIHTV-SDELEQRNTELKLFINVGIDDHLNKKK 180

Query: 181 QSHTRWKSIQFNHPSTFESISMEPDLKEKVKSDLESFLKSKQYYHKLGRVWKRSYLLYGP 240
           + + RW+SI F+HP TF++I+ME DLK KVKSDLESFLK KQYY++LGRVWKRSYLLYGP
Sbjct: 181 KKNGRWRSIPFDHPCTFDNIAMETDLKNKVKSDLESFLKGKQYYNRLGRVWKRSYLLYGP 240

Query: 241 SGTGKSSFVAAMANFLSYDVYDIDLFKVSDESDLKLLLLQTTSKSIIVVEDLDRFLIEKS 300
           SGTGKSSFVAAMANFL YDVYDIDL KV D+SDLK+LLLQT  KS+IV+EDLDR L  KS
Sbjct: 241 SGTGKSSFVAAMANFLDYDVYDIDLSKVVDDSDLKMLLLQTRGKSVIVIEDLDRHLSTKS 300

Query: 301 PALSLSALLNFMDGILTSCCAEERMMVFTVNCKEQVDPAILRPGRIDVHIHFPLCDFSAF 360
            A++LS +LNF D IL+SC A+ER+MVFT+  KEQ+DPA+LRPGR+DVHIHFPLCDF+AF
Sbjct: 301 TAVNLSGILNFTDSILSSCTADERIMVFTMTGKEQIDPAMLRPGRVDVHIHFPLCDFTAF 360

Query: 361 KNLAINYLGVKDHKLFPQVEEIFQTGASLSPAEIGELMIANRSSPSRAIKSVISALQTDG 420
           K LA NYLGVK+HKLF QVE IFQ GASLSPAEIGELMIANR+SP+RA+K VI+ALQTDG
Sbjct: 361 KTLANNYLGVKEHKLFSQVEGIFQNGASLSPAEIGELMIANRNSPTRALKHVINALQTDG 420

Query: 421 ERRRVGNMGRRLSNCGSRKSIDESV--ESSGVFCSENAHTG---KEFRKLYGFLRMKSNK 480
           +RR  G   R L   GSRKS  E V  + SG  C     +    KEFRKLYG LR+KS++
Sbjct: 421 DRRGTGR--RLLLENGSRKSTSEDVSDDMSGSLCGGGGGSSPAVKEFRKLYGLLRIKSSR 480

Query: 481 ISQSFDQSPRHKDG 482
            S SFD +   +DG
Sbjct: 481 KSGSFDVAREMRDG 491

BLAST of Moc05g04850 vs. ExPASy Swiss-Prot
Match: Q9LJJ7 (AAA-ATPase At3g28580 OS=Arabidopsis thaliana OX=3702 GN=At3g28580 PE=2 SV=1)

HSP 1 Score: 248.1 bits (632), Expect = 2.1e-64
Identity = 153/437 (35.01%), Postives = 241/437 (55.15%), Query Frame = 0

Query: 33  LVKKWWRNLQDCFHVYQSLRIPEFNESSQRNLLYWKVSAYLSSLSSLEDSDF-TNLVTGK 92
           L  +++  +Q  FH Y         E  +R+  Y  + +YLS  SS        N   G 
Sbjct: 43  LFGRFYPYIQITFHEYS-------GEHFKRSEAYLGIQSYLSKDSSARAKKLKANTTKGS 102

Query: 93  KPNDILLRLDSNQTVVDEFLGARLLWTNEENGS--------------RNFVLKIRRADKR 152
           K   I+L +D  + + D+F G R+ W +++ G+              R ++L+  R D+ 
Sbjct: 103 K--SIVLSMDDKEEITDDFEGIRVWWQSKKEGATRQSFSFYPEANEKRYYMLRFHRRDRE 162

Query: 153 RILRPYLQHIHALTADEIEHNKGDLRLFMNSKFNNQSH---TRWKSIQFNHPSTFESISM 212
            I+  YL+H+       IE    + +L+ N+    QSH   ++W  + F HP+TF++++M
Sbjct: 163 VIIERYLEHV-MREGKTIEQKNRERKLYSNTP--GQSHGNNSKWSHVTFEHPATFDTLAM 222

Query: 213 EPDLKEKVKSDLESFLKSKQYYHKLGRVWKRSYLLYGPSGTGKSSFVAAMANFLSYDVYD 272
           E + KE++KSDL  F KSK YY K+G+ WKR YLL+GP GTGKS+ +AAMANFL YDVYD
Sbjct: 223 EENKKEEIKSDLIKFSKSKDYYKKIGKAWKRGYLLFGPPGTGKSTMIAAMANFLEYDVYD 282

Query: 273 IDLFKVSDESDLKLLLLQTTSKSIIVVEDLDRFL---------------------IE--- 332
           ++L  V D + L+ LL++T++KSIIV+ED+D  L                     IE   
Sbjct: 283 LELTTVKDNTHLRRLLIETSAKSIIVIEDIDCSLNLTGQRKKKEEEEEDGDDKNTIEKKM 342

Query: 333 --------KSPALSLSALLNFMDGILTSCCAEERMMVFTVNCKEQVDPAILRPGRIDVHI 392
                   K   ++LS LLNF+DG L S C  ER++VFT N  +++DPA++R GR+D HI
Sbjct: 343 MMKNEGENKESKVTLSGLLNFIDG-LWSACGGERIIVFTTNFVDKLDPALIRKGRMDKHI 402

Query: 393 HFPLCDFSAFKNLAINYLGVKDHKLFPQVEEIFQT-GASLSPAEIGELMI--ANRSSPSR 417
               C F AFK LA NYL V++ ++F +++ + +     ++PA++GE ++  + +     
Sbjct: 403 EMSYCCFEAFKVLAKNYLDVEESEMFEEIKRLLEVEEIKMTPADVGENLLPKSEKEGGET 462

BLAST of Moc05g04850 vs. ExPASy Swiss-Prot
Match: Q9FLD5 (AAA-ATPase ASD, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=AATP1 PE=1 SV=1)

HSP 1 Score: 244.6 bits (623), Expect = 2.3e-63
Identity = 158/453 (34.88%), Postives = 247/453 (54.53%), Query Frame = 0

Query: 41  LQDCFHVYQSLRIPEFNESSQRNLLYWKVSAYLSSLSSLEDSDFT-NLVTGKKPNDILLR 100
           +Q  FH Y         E  +R+ +Y  + +YLS  SS      T N + G K   I+L 
Sbjct: 52  IQITFHEYS-------GERFKRSDVYDAIQSYLSKDSSSRAKKLTANTIKGNK--SIILS 111

Query: 101 LDSNQTVVDEFLGARLLWTNEE--------------NGSRNFVLKIRRADKRRILRPYLQ 160
           +D ++ + DEF G ++ W +++              + SR ++LK  R D+  I + YL 
Sbjct: 112 MDDHEEITDEFQGVKVWWQSKKHQSESRAISFYPKADESRFYMLKFHRRDREVITKKYLN 171

Query: 161 HIHALTADEIEHNKGDLRLFMNSKFNNQS---HTRWKSIQFNHPSTFESISMEPDLKEKV 220
           H+ +     IE    + +L+ N+   N S    T+W  + F HP+TF++++ME   KE++
Sbjct: 172 HVIS-EGKTIEVKNRERKLYSNNPSQNWSGYKQTKWSHVTFEHPATFDTLAMEYKKKEEI 231

Query: 221 KSDLESFLKSKQYYHKLGRVWKRSYLLYGPSGTGKSSFVAAMANFLSYDVYDIDLFKVSD 280
           K+DL  F  SK YY K+G+ WKR YLL+GP GTGKS+ +AAMAN L YDVYD++L  V D
Sbjct: 232 KNDLIKFSNSKDYYKKIGKAWKRGYLLFGPPGTGKSTMIAAMANLLEYDVYDLELTTVKD 291

Query: 281 ESDLKLLLLQTTSKSIIVVEDLDRFL---------------------IE----------K 340
            ++L+ LL++T+ KSIIV+ED+D  L                     IE          K
Sbjct: 292 NTELRRLLIETSGKSIIVIEDIDCSLDLTGQRKQKKDEEEDEDETSPIEKQMKKDQGENK 351

Query: 341 SPALSLSALLNFMDGILTSCCAEERMMVFTVNCKEQVDPAILRPGRIDVHIHFPLCDFSA 400
              ++LS LLNF+DG L S C  ER++VFT N  +++DPA++R GR+D HI    C F A
Sbjct: 352 GSKVTLSGLLNFIDG-LWSACGGERIIVFTTNFIDKLDPALIRKGRMDKHIEMSYCGFEA 411

Query: 401 FKNLAINYLGVK---DHKLFPQVEEIFQT-GASLSPAEIGELMIANRSSPSRAI--KSVI 439
           FK LA NYL  K   D++LF +++ + +     ++PA++GE ++      ++ I  K +I
Sbjct: 412 FKVLANNYLDAKEEDDNELFDEIKRLLEVEEIKMTPADVGENLLKKSEVETKEICLKRLI 471

BLAST of Moc05g04850 vs. ExPASy Swiss-Prot
Match: Q9FKM3 (AAA-ATPase At5g57480 OS=Arabidopsis thaliana OX=3702 GN=At5g57480 PE=3 SV=1)

HSP 1 Score: 238.8 bits (608), Expect = 1.3e-61
Identity = 163/472 (34.53%), Postives = 247/472 (52.33%), Query Frame = 0

Query: 1   MELFFRLFCLILIGICFTWLLRIVLFRTGLIFLVKKWWRNLQDCFHVYQSLRIPEFNESS 60
           M+ ++     +L  + F   L   +F   L F   K++  +   F  Y    I E  +  
Sbjct: 1   MKEYWTSLASLLGVLAFCQSLMQSIFPPELRFAFLKFFNRIFHVFSSYCYFDITEI-DGV 60

Query: 61  QRNLLYWKVSAYLSSLSSLEDSDFTNLVTGKKPNDILLRLDSNQTVVDEFLGARLLWTNE 120
             N LY  V  YLSS  S+  +   +L      + I   L +N ++VD F G  +LW + 
Sbjct: 61  NTNELYNAVQLYLSSSVSIAGNRL-SLTRAVNSSSITFGLSNNDSIVDTFNGVTVLWEHV 120

Query: 121 --------------ENGSRNFVLKIRRADKRRILRPYLQHIHALTADEIEHNKGDLRLFM 180
                             R F L+I++ DK  IL  YL +I    A+EI     D  L+ 
Sbjct: 121 VTQRQTQTFAWRPLPEEKRGFTLRIKKKDKTLILNSYLDYIME-RANEIRRKNQDRLLYT 180

Query: 181 NSK---FNNQSHTRWKSIQFNHPSTFESISMEPDLKEKVKSDLESFLKSKQYYHKLGRVW 240
           NS+    +++ H  W+S+ F HPSTFE+++M+P  K+++  DL+ F + + +Y K GR W
Sbjct: 181 NSRGGSLDSRGHP-WESVPFKHPSTFETLAMDPRKKQQIMDDLKDFAEGQVFYQKTGRAW 240

Query: 241 KRSYLLYGPSGTGKSSFVAAMANFLSYDVYDIDLFKVSDESDLKLLLLQTTSKSIIVVED 300
           KR YLLYGP GTGKSS +AAMAN+L YD+YD++L +V   S+L+ LL++T+SKSIIV+ED
Sbjct: 241 KRGYLLYGPPGTGKSSMIAAMANYLGYDIYDLELTEVHSNSELRKLLMKTSSKSIIVIED 300

Query: 301 LDRFLI-------------------------------------EKSPALSLSALLNFMDG 360
           +D  +                                           ++LS LLNF DG
Sbjct: 301 IDCSINLTNRKKNSSNVSSQRSYYDAETRNGSGSGSGGSGEEGGNGNTITLSGLLNFTDG 360

Query: 361 ILTSCCAEERMMVFTVNCKEQVDPAILRPGRIDVHIHFPLCDFSAFKNLAINYL--GVKD 415
            L SCC  ER+ VFT N  E++DPA+LR GR+D+HI+   C+F + K L  NYL  GV+D
Sbjct: 361 -LWSCCGSERIFVFTTNHIEKLDPALLRSGRMDMHIYMSFCNFPSLKILLKNYLGYGVED 420

BLAST of Moc05g04850 vs. ExPASy Swiss-Prot
Match: F4JPK8 (AAA-ATPase At4g30250 OS=Arabidopsis thaliana OX=3702 GN=At4g30250 PE=3 SV=1)

HSP 1 Score: 237.3 bits (604), Expect = 3.7e-61
Identity = 159/464 (34.27%), Postives = 247/464 (53.23%), Query Frame = 0

Query: 8   FCLILIGICFTWLLRIVLFRTGLIFLVKKWWRNLQDCFHVYQSLRIPEFNESSQRNLLYW 67
           FC  ++ + F   LR+      L FL +     ++  F  +    I E  +    N LY 
Sbjct: 17  FCQTIVQLVFPPELRLAF----LHFLTR-----IRHVFSSHIYFDITEI-DGVNTNELYN 76

Query: 68  KVSAYLSSLSSLED-----SDFTNLVTGKKPN--DILLRLDSNQTVVDEFLGARLLWTN- 127
            V  YLSS  ++ D     ++ T L   + PN   +   L +N  + D F G  +LW + 
Sbjct: 77  AVQLYLSSSVTVNDAVSSSNNNTRLSLTRVPNSSSVTFGLSNNDRITDVFNGVTILWEHV 136

Query: 128 -------------EENGSRNFVLKIRRADKRRILRPYLQHIHALTADEIEHNKGDLRLFM 187
                             R F L+I + DK  +L  YL +I    ++EI     +  L+ 
Sbjct: 137 VVQRQVQSFSWRPMPEEKRGFTLQINKRDKALVLDSYLDYIVG-KSEEIRRRNEERLLYT 196

Query: 188 NSK---FNNQSHTRWKSIQFNHPSTFESISMEPDLKEKVKSDLESFLKSKQYYHKLGRVW 247
           NS+    + +SH  W S++F HPSTF++++M+P+ K+++  DL  F   + +Y K GR W
Sbjct: 197 NSRGVSLDARSHP-WDSVRFKHPSTFDTLAMDPEKKKRIMEDLREFANGQGFYQKTGRAW 256

Query: 248 KRSYLLYGPSGTGKSSFVAAMANFLSYDVYDIDLFKVSDESDLKLLLLQTTSKSIIVVED 307
           KR YLLYGP GTGKSS +AAMAN+L YD+YD++L +V + S+L+ LL++T+SKSIIV+ED
Sbjct: 257 KRGYLLYGPPGTGKSSLIAAMANYLGYDIYDLELTEVQNNSELRKLLMKTSSKSIIVIED 316

Query: 308 LD---------------------------RFLIEKSPALSLSALLNFMDGILTSCCAEER 367
           +D                             L E   +++LS LLNF DG L SCC  E+
Sbjct: 317 IDCSISLTKRGKNKKKNGSYEYDPGLTNGSGLEEPGSSVTLSGLLNFTDG-LWSCCGSEK 376

Query: 368 MMVFTVNCKEQVDPAILRPGRIDVHIHFPLCDFSAFKNLAINYLGVK----DHKLFPQVE 417
           + VFT N  E++D A++R GR+D+H+H   C F A K L  NYL ++    D  +  ++E
Sbjct: 377 IFVFTTNHIEKLDSALMRSGRMDMHVHMGFCKFPALKILLKNYLRLEEEDMDSVVLKEME 436

BLAST of Moc05g04850 vs. ExPASy TrEMBL
Match: A0A1S3AVK0 (AAA-ATPase At2g46620 OS=Cucumis melo OX=3656 GN=LOC103483161 PE=3 SV=1)

HSP 1 Score: 831.2 bits (2146), Expect = 2.1e-237
Identity = 418/480 (87.08%), Postives = 447/480 (93.12%), Query Frame = 0

Query: 1   MELFFRLFCLILIGICFTWLLRIVLFRTGLIFLVKKWWRNLQDCFHVYQSLRIPEFNESS 60
           MELFFR F +I IGICF+WLLRI+LFRTGLIF+VKKWW NL+DCFHVYQS RIPEFNE+S
Sbjct: 1   MELFFRFFYVIFIGICFSWLLRILLFRTGLIFIVKKWWANLEDCFHVYQSFRIPEFNETS 60

Query: 61  QRNLLYWKVSAYLSSLSSLEDSDFTNLVTGKKPNDILLRLDSNQTVVDEFLGARLLWTNE 120
           Q N LY KVSAYL+SLSSLEDSD+TNL+TG KPNDI+LRLDSNQTV D FLGA++ WTNE
Sbjct: 61  QHNHLYRKVSAYLTSLSSLEDSDYTNLITGNKPNDIILRLDSNQTVQDNFLGAKVFWTNE 120

Query: 121 ENGSRNFVLKIRRADKRRILRPYLQHIHALTADEIEHNKGDLRLFMNSKFNNQSHTRWKS 180
           +NGSRNFVLKIR+ADKRRILRPYLQHIH LTAD+ E  KGDL+LFMNSK NN S TRWKS
Sbjct: 121 QNGSRNFVLKIRKADKRRILRPYLQHIHTLTADDNEQRKGDLKLFMNSKPNNHSDTRWKS 180

Query: 181 IQFNHPSTFESISMEPDLKEKVKSDLESFLKSKQYYHKLGRVWKRSYLLYGPSGTGKSSF 240
           IQF HPSTF+SI+ME DLKEKVKSDLESFLKSKQYYH+LGRVWKRSYLLYGPSGTGKSSF
Sbjct: 181 IQFKHPSTFDSIAMETDLKEKVKSDLESFLKSKQYYHRLGRVWKRSYLLYGPSGTGKSSF 240

Query: 241 VAAMANFLSYDVYDIDLFKVSDESDLKLLLLQTTSKSIIVVEDLDRFLIEKSPALSLSAL 300
           VAAMANFLSYDVYDIDLFKVSD+SDLK LLLQTTSKS+IVVEDLDRFLIEKS ALSLSAL
Sbjct: 241 VAAMANFLSYDVYDIDLFKVSDDSDLKFLLLQTTSKSVIVVEDLDRFLIEKSSALSLSAL 300

Query: 301 LNFMDGILTSCCAEERMMVFTVNCKEQVDPAILRPGRIDVHIHFPLCDFSAFKNLAINYL 360
           LNFMDGILTSCCAEER+MVFTVNCKEQV+PAILRPGRIDVHIHFPLCDFSAFKNLAINYL
Sbjct: 301 LNFMDGILTSCCAEERVMVFTVNCKEQVEPAILRPGRIDVHIHFPLCDFSAFKNLAINYL 360

Query: 361 GVKDHKLFPQVEEIFQTGASLSPAEIGELMIANRSSPSRAIKSVISALQTDGERRRVGNM 420
           GVKDHKLFPQVEEIFQTGASLSPAEI ELMIANR+SPSRAIKSVISALQTDG+RRRV N+
Sbjct: 361 GVKDHKLFPQVEEIFQTGASLSPAEISELMIANRNSPSRAIKSVISALQTDGDRRRVSNI 420

Query: 421 GRRLSNCGSRKSIDESVESSGVFCSENAHTGKEFRKLYGFLRMKSNKISQSFDQSPRHKD 480
           GRRLS+CGSRKS+ ES+ES GV CSEN  TGKEFRKLYGFLRMKSNKISQSFD SP  KD
Sbjct: 421 GRRLSDCGSRKSVAESIESGGVLCSENTQTGKEFRKLYGFLRMKSNKISQSFDSSPIRKD 480

BLAST of Moc05g04850 vs. ExPASy TrEMBL
Match: A0A0A0L5T8 (AAA domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G119330 PE=3 SV=1)

HSP 1 Score: 828.6 bits (2139), Expect = 1.4e-236
Identity = 418/480 (87.08%), Postives = 445/480 (92.71%), Query Frame = 0

Query: 1   MELFFRLFCLILIGICFTWLLRIVLFRTGLIFLVKKWWRNLQDCFHVYQSLRIPEFNESS 60
           MELFFR F  I IGICF+WLLRI+LFRTGLIFLVKKWW NL+DCFHVYQS RIPEFNE+S
Sbjct: 1   MELFFRFFSAIFIGICFSWLLRILLFRTGLIFLVKKWWANLEDCFHVYQSFRIPEFNETS 60

Query: 61  QRNLLYWKVSAYLSSLSSLEDSDFTNLVTGKKPNDILLRLDSNQTVVDEFLGARLLWTNE 120
           Q N LY KVSAYL+SLSSLEDSDFTNL+TG KPNDI+LRLDSNQTV D FLGA++ WTNE
Sbjct: 61  QHNHLYRKVSAYLTSLSSLEDSDFTNLITGNKPNDIILRLDSNQTVQDNFLGAKVFWTNE 120

Query: 121 ENGSRNFVLKIRRADKRRILRPYLQHIHALTADEIEHNKGDLRLFMNSKFNNQSHTRWKS 180
           + GSRNFVL+IR+ADKRRILRPYLQHIH LTADE E  KGDL+LFMNSK NN S TRWKS
Sbjct: 121 QKGSRNFVLRIRKADKRRILRPYLQHIHTLTADENEQRKGDLKLFMNSKPNNHSDTRWKS 180

Query: 181 IQFNHPSTFESISMEPDLKEKVKSDLESFLKSKQYYHKLGRVWKRSYLLYGPSGTGKSSF 240
           IQF HPSTF+SI+ME DLKEKVKSDLESFLKSKQYYH+LGRVWKRSYLLYGPSGTGKSSF
Sbjct: 181 IQFKHPSTFDSIAMETDLKEKVKSDLESFLKSKQYYHRLGRVWKRSYLLYGPSGTGKSSF 240

Query: 241 VAAMANFLSYDVYDIDLFKVSDESDLKLLLLQTTSKSIIVVEDLDRFLIEKSPALSLSAL 300
           VAAMANFLSYDVYDIDLFKVSD+SDLK LLLQTTSKS+IVVEDLDRFLIEKS ALSLSAL
Sbjct: 241 VAAMANFLSYDVYDIDLFKVSDDSDLKFLLLQTTSKSVIVVEDLDRFLIEKSSALSLSAL 300

Query: 301 LNFMDGILTSCCAEERMMVFTVNCKEQVDPAILRPGRIDVHIHFPLCDFSAFKNLAINYL 360
           LNFMDGILTSCCAEER+MVFTVNCKEQV+PAILRPGRIDVHIHFPLCDFSAFKNLAINYL
Sbjct: 301 LNFMDGILTSCCAEERVMVFTVNCKEQVEPAILRPGRIDVHIHFPLCDFSAFKNLAINYL 360

Query: 361 GVKDHKLFPQVEEIFQTGASLSPAEIGELMIANRSSPSRAIKSVISALQTDGERRRVGNM 420
           GVKDHKLFPQVEEIFQTGASLSPAEI ELMIANR+SPSRAIKSVISALQTDG+RRRV N+
Sbjct: 361 GVKDHKLFPQVEEIFQTGASLSPAEISELMIANRNSPSRAIKSVISALQTDGDRRRVSNI 420

Query: 421 GRRLSNCGSRKSIDESVESSGVFCSENAHTGKEFRKLYGFLRMKSNKISQSFDQSPRHKD 480
           GRRLS+CGSRKS+ ES+ES GV CSEN  TGKEFRKLYGFLRMKSNKISQSFD SP  K+
Sbjct: 421 GRRLSDCGSRKSVAESIESGGVVCSENTQTGKEFRKLYGFLRMKSNKISQSFDSSPIRKE 480

BLAST of Moc05g04850 vs. ExPASy TrEMBL
Match: A0A6J1E8T1 (AAA-ATPase At2g46620-like OS=Cucurbita moschata OX=3662 GN=LOC111431901 PE=3 SV=1)

HSP 1 Score: 808.1 bits (2086), Expect = 1.9e-230
Identity = 409/480 (85.21%), Postives = 441/480 (91.88%), Query Frame = 0

Query: 1   MELFFRLFCLILIGICFTWLLRIVLFRTGLIFLVKKWWRNLQDCFHVYQSLRIPEFNESS 60
           MELFFR F +I IGICF WLLRIVLFRTG +F+VKK WRNL+DCFHVYQS RIPEFN+SS
Sbjct: 1   MELFFRSFFVIFIGICFCWLLRIVLFRTGFVFVVKKRWRNLEDCFHVYQSFRIPEFNQSS 60

Query: 61  QRNLLYWKVSAYLSSLSSLEDSDFTNLVTGKKPNDILLRLDSNQTVVDEFLGARLLWTNE 120
           Q+N LY KVSAYL+SLSSLEDSDFTNL+TG  PNDI+LRLDSNQTV D FLGA+LLWTNE
Sbjct: 61  QQNHLYRKVSAYLTSLSSLEDSDFTNLITGNAPNDIILRLDSNQTVQDNFLGAKLLWTNE 120

Query: 121 ENGSRNFVLKIRRADKRRILRPYLQHIHALTADEIEHNKGDLRLFMNSKFNNQSHTRWKS 180
           ENGSRNFVL+IR+ADKRRILRPYLQHIH LTAD IE  KGDL+LFMNSK NNQS TRWKS
Sbjct: 121 ENGSRNFVLRIRKADKRRILRPYLQHIHTLTADVIEQRKGDLKLFMNSKPNNQSDTRWKS 180

Query: 181 IQFNHPSTFESISMEPDLKEKVKSDLESFLKSKQYYHKLGRVWKRSYLLYGPSGTGKSSF 240
           IQF HPSTF+SISME DLKE+VKSDLESFLKSKQYYH+LGRVWKRSYLLYGPSGTGKSSF
Sbjct: 181 IQFKHPSTFDSISMETDLKERVKSDLESFLKSKQYYHRLGRVWKRSYLLYGPSGTGKSSF 240

Query: 241 VAAMANFLSYDVYDIDLFKVSDESDLKLLLLQTTSKSIIVVEDLDRFLIEKSPALSLSAL 300
           VAAMANFLSY+VYDIDLFKVSD+SDLKLLLLQTT+KSIIVVEDLDRFL++KS  LSLSAL
Sbjct: 241 VAAMANFLSYNVYDIDLFKVSDDSDLKLLLLQTTTKSIIVVEDLDRFLMDKSSTLSLSAL 300

Query: 301 LNFMDGILTSCCAEERMMVFTVNCKEQVDPAILRPGRIDVHIHFPLCDFSAFKNLAINYL 360
           LNFMDGILTSCCAEER+MVFTVNCK+ VDPA+LRPGRIDVHIHFPLCDFSAFKNLA NYL
Sbjct: 301 LNFMDGILTSCCAEERVMVFTVNCKDLVDPAVLRPGRIDVHIHFPLCDFSAFKNLANNYL 360

Query: 361 GVKDHKLFPQVEEIFQTGASLSPAEIGELMIANRSSPSRAIKSVISALQTDGERRRVGNM 420
           GVKDHKLFPQVEEIFQ GASLSPAEI ELMI NR+SPSRAIKSVI+ALQTD ERRR+ ++
Sbjct: 361 GVKDHKLFPQVEEIFQNGASLSPAEISELMITNRNSPSRAIKSVITALQTDVERRRISSI 420

Query: 421 GRRLSNCGSRKSIDESVESSGVFCSENAHTGKEFRKLYGFLRMKSNKISQSFDQSPRHKD 480
           GRRLS+CGSRKS+ ESVES GV CSEN  TGKE RKLYGFLRMKSNKISQ+FD SP  KD
Sbjct: 421 GRRLSDCGSRKSVAESVESGGVLCSENTQTGKELRKLYGFLRMKSNKISQTFDTSPMRKD 480

BLAST of Moc05g04850 vs. ExPASy TrEMBL
Match: A0A6J1IWA6 (AAA-ATPase At2g46620 OS=Cucurbita maxima OX=3661 GN=LOC111479067 PE=3 SV=1)

HSP 1 Score: 806.6 bits (2082), Expect = 5.6e-230
Identity = 407/480 (84.79%), Postives = 440/480 (91.67%), Query Frame = 0

Query: 1   MELFFRLFCLILIGICFTWLLRIVLFRTGLIFLVKKWWRNLQDCFHVYQSLRIPEFNESS 60
           MELFFR F  I IGICF WLLRIVLFRTG +F+VKKWWRNL+DCFHVYQ  RIPEFN+SS
Sbjct: 1   MELFFRSFFAIFIGICFCWLLRIVLFRTGFVFVVKKWWRNLEDCFHVYQFFRIPEFNQSS 60

Query: 61  QRNLLYWKVSAYLSSLSSLEDSDFTNLVTGKKPNDILLRLDSNQTVVDEFLGARLLWTNE 120
           Q+N LY KVSAYL+SLSSLEDSDFTNL+TG  PNDI+LRLDSNQTV D FLGA+LLWTNE
Sbjct: 61  QQNHLYRKVSAYLTSLSSLEDSDFTNLITGNTPNDIILRLDSNQTVQDNFLGAKLLWTNE 120

Query: 121 ENGSRNFVLKIRRADKRRILRPYLQHIHALTADEIEHNKGDLRLFMNSKFNNQSHTRWKS 180
           E GSRNFVL+IR+ADKRRILRPYLQHIH LTAD IE  KGDL+LFMNSK NNQS TRWKS
Sbjct: 121 EYGSRNFVLRIRKADKRRILRPYLQHIHTLTADVIEQRKGDLKLFMNSKPNNQSDTRWKS 180

Query: 181 IQFNHPSTFESISMEPDLKEKVKSDLESFLKSKQYYHKLGRVWKRSYLLYGPSGTGKSSF 240
           IQF HPSTF+SISME DLKE+VKSDLESFLKSKQYYH+LGRVWKRSYLLYGPSGTGKSSF
Sbjct: 181 IQFQHPSTFDSISMETDLKERVKSDLESFLKSKQYYHRLGRVWKRSYLLYGPSGTGKSSF 240

Query: 241 VAAMANFLSYDVYDIDLFKVSDESDLKLLLLQTTSKSIIVVEDLDRFLIEKSPALSLSAL 300
           VAAMANFLSY+VYDIDLFKVSD+SDLKLLLLQTT+KSIIVVEDLDRFL++KS ALSLSAL
Sbjct: 241 VAAMANFLSYNVYDIDLFKVSDDSDLKLLLLQTTTKSIIVVEDLDRFLMDKSSALSLSAL 300

Query: 301 LNFMDGILTSCCAEERMMVFTVNCKEQVDPAILRPGRIDVHIHFPLCDFSAFKNLAINYL 360
           LNFMDG+LTSCCAEER+MVFTVNCK+ VDPA+LRPGRIDVHIHFPLCDFSAFKNLA NYL
Sbjct: 301 LNFMDGLLTSCCAEERVMVFTVNCKDLVDPAVLRPGRIDVHIHFPLCDFSAFKNLANNYL 360

Query: 361 GVKDHKLFPQVEEIFQTGASLSPAEIGELMIANRSSPSRAIKSVISALQTDGERRRVGNM 420
           GVKDHKLFPQVEEIFQ GASLSPAEI ELMI NR+SPSRAIKSVI+ALQTD ERRR+ ++
Sbjct: 361 GVKDHKLFPQVEEIFQNGASLSPAEISELMITNRNSPSRAIKSVITALQTDVERRRISSI 420

Query: 421 GRRLSNCGSRKSIDESVESSGVFCSENAHTGKEFRKLYGFLRMKSNKISQSFDQSPRHKD 480
           GRRLS+CGSRKS+ ESVES GV CSEN  TGKE RKLYGFLRMKSNKISQ+FD SP  K+
Sbjct: 421 GRRLSDCGSRKSVAESVESGGVLCSENTQTGKELRKLYGFLRMKSNKISQTFDASPMRKE 480

BLAST of Moc05g04850 vs. ExPASy TrEMBL
Match: A0A5A7U3P9 (AAA-ATPase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold291G00800 PE=3 SV=1)

HSP 1 Score: 798.9 bits (2062), Expect = 1.2e-227
Identity = 399/457 (87.31%), Postives = 426/457 (93.22%), Query Frame = 0

Query: 24  VLFRTGLIFLVKKWWRNLQDCFHVYQSLRIPEFNESSQRNLLYWKVSAYLSSLSSLEDSD 83
           + FRTGLIF+VKKWW NL+DCFHVYQS RIPEFNE+SQ N LY KVSAYL+SLSSLEDSD
Sbjct: 3   LFFRTGLIFIVKKWWANLEDCFHVYQSFRIPEFNETSQHNHLYRKVSAYLTSLSSLEDSD 62

Query: 84  FTNLVTGKKPNDILLRLDSNQTVVDEFLGARLLWTNEENGSRNFVLKIRRADKRRILRPY 143
           +TNL+TG KPNDI+LRLDSNQTV D FLGA++ WTNE+NGSRNFVLKIR+ADKRRILRPY
Sbjct: 63  YTNLITGNKPNDIILRLDSNQTVQDNFLGAKVFWTNEQNGSRNFVLKIRKADKRRILRPY 122

Query: 144 LQHIHALTADEIEHNKGDLRLFMNSKFNNQSHTRWKSIQFNHPSTFESISMEPDLKEKVK 203
           LQHIH LTAD+ E  KGDL+LFMNSK NN S TRWKSIQF HPSTF+SI+ME DLKEKVK
Sbjct: 123 LQHIHTLTADDNEQRKGDLKLFMNSKPNNHSDTRWKSIQFKHPSTFDSIAMETDLKEKVK 182

Query: 204 SDLESFLKSKQYYHKLGRVWKRSYLLYGPSGTGKSSFVAAMANFLSYDVYDIDLFKVSDE 263
           SDLESFLKSKQYYH+LGRVWKRSYLLYGPSGTGKSSFVAAMANFLSYDVYDIDLFKVSD+
Sbjct: 183 SDLESFLKSKQYYHRLGRVWKRSYLLYGPSGTGKSSFVAAMANFLSYDVYDIDLFKVSDD 242

Query: 264 SDLKLLLLQTTSKSIIVVEDLDRFLIEKSPALSLSALLNFMDGILTSCCAEERMMVFTVN 323
           SDLK LLLQTTSKS+IVVEDLDRFLIEKS ALSLSALLNFMDGILTSCCAEER+MVFTVN
Sbjct: 243 SDLKFLLLQTTSKSVIVVEDLDRFLIEKSSALSLSALLNFMDGILTSCCAEERVMVFTVN 302

Query: 324 CKEQVDPAILRPGRIDVHIHFPLCDFSAFKNLAINYLGVKDHKLFPQVEEIFQTGASLSP 383
           CKEQV+PAILRPGRIDVHIHFPLCDFSAFKNLAINYLGVKDHKLFPQVEEIFQTGASLSP
Sbjct: 303 CKEQVEPAILRPGRIDVHIHFPLCDFSAFKNLAINYLGVKDHKLFPQVEEIFQTGASLSP 362

Query: 384 AEIGELMIANRSSPSRAIKSVISALQTDGERRRVGNMGRRLSNCGSRKSIDESVESSGVF 443
           AEI ELMIANR+SPSRAIKSVISALQTDG+RRRV N+GRRLS+CGSRKS+ ES+ES GV 
Sbjct: 363 AEISELMIANRNSPSRAIKSVISALQTDGDRRRVSNIGRRLSDCGSRKSVAESIESGGVL 422

Query: 444 CSENAHTGKEFRKLYGFLRMKSNKISQSFDQSPRHKD 481
           CSEN  TGKEFRKLYGFLRMKSNKISQSFD SP  KD
Sbjct: 423 CSENTQTGKEFRKLYGFLRMKSNKISQSFDSSPIRKD 459

BLAST of Moc05g04850 vs. TAIR 10
Match: AT2G46620.1 (P-loop containing nucleoside triphosphate hydrolases superfamily protein )

HSP 1 Score: 583.6 bits (1503), Expect = 1.5e-166
Identity = 314/494 (63.56%), Postives = 384/494 (77.73%), Query Frame = 0

Query: 1   MELFFRLFCLILIGICFTWLLRIVLFRTGLIFLVKKWWRNLQDCFHVYQSLRIPEFNESS 60
           M + +  F L+L+     +L+RI+LF+TGLI++VK W R + D FHVYQ  ++PEFN++ 
Sbjct: 1   MGILWDSFLLLLVSTFALFLVRILLFKTGLIYMVKLWRRKIIDWFHVYQFYKVPEFNDNV 60

Query: 61  QRNLLYWKVSAYLSSLSSLEDSDFTNLVTGKKPNDILLRLDSNQTVVDEFLGARLLWTN- 120
           Q N LY KV  YL+SLSS+E+SDFTNL TGKK N+I+LRLD NQ V DEFLGAR+ W N 
Sbjct: 61  QENHLYQKVYMYLNSLSSIENSDFTNLFTGKKSNEIILRLDRNQVVGDEFLGARVCWING 120

Query: 121 -EENGSRNFVLKIRRADKRRILRPYLQHIHALTADEIEHNKGDLRLFMNSKFNN------ 180
            +E+G+RNFVLKIR+ADKRRIL  YLQHIH + +DE+E    +L+LF+N   ++      
Sbjct: 121 EDEDGARNFVLKIRKADKRRILGSYLQHIHTV-SDELEQRNTELKLFINVGIDDHLNKKK 180

Query: 181 QSHTRWKSIQFNHPSTFESISMEPDLKEKVKSDLESFLKSKQYYHKLGRVWKRSYLLYGP 240
           + + RW+SI F+HP TF++I+ME DLK KVKSDLESFLK KQYY++LGRVWKRSYLLYGP
Sbjct: 181 KKNGRWRSIPFDHPCTFDNIAMETDLKNKVKSDLESFLKGKQYYNRLGRVWKRSYLLYGP 240

Query: 241 SGTGKSSFVAAMANFLSYDVYDIDLFKVSDESDLKLLLLQTTSKSIIVVEDLDRFLIEKS 300
           SGTGKSSFVAAMANFL YDVYDIDL KV D+SDLK+LLLQT  KS+IV+EDLDR L  KS
Sbjct: 241 SGTGKSSFVAAMANFLDYDVYDIDLSKVVDDSDLKMLLLQTRGKSVIVIEDLDRHLSTKS 300

Query: 301 PALSLSALLNFMDGILTSCCAEERMMVFTVNCKEQVDPAILRPGRIDVHIHFPLCDFSAF 360
            A++LS +LNF D IL+SC A+ER+MVFT+  KEQ+DPA+LRPGR+DVHIHFPLCDF+AF
Sbjct: 301 TAVNLSGILNFTDSILSSCTADERIMVFTMTGKEQIDPAMLRPGRVDVHIHFPLCDFTAF 360

Query: 361 KNLAINYLGVKDHKLFPQVEEIFQTGASLSPAEIGELMIANRSSPSRAIKSVISALQTDG 420
           K LA NYLGVK+HKLF QVE IFQ GASLSPAEIGELMIANR+SP+RA+K VI+ALQTDG
Sbjct: 361 KTLANNYLGVKEHKLFSQVEGIFQNGASLSPAEIGELMIANRNSPTRALKHVINALQTDG 420

Query: 421 ERRRVGNMGRRLSNCGSRKSIDESV--ESSGVFCSENAHTG---KEFRKLYGFLRMKSNK 480
           +RR  G   R L   GSRKS  E V  + SG  C     +    KEFRKLYG LR+KS++
Sbjct: 421 DRRGTGR--RLLLENGSRKSTSEDVSDDMSGSLCGGGGGSSPAVKEFRKLYGLLRIKSSR 480

Query: 481 ISQSFDQSPRHKDG 482
            S SFD +   +DG
Sbjct: 481 KSGSFDVAREMRDG 491

BLAST of Moc05g04850 vs. TAIR 10
Match: AT3G28580.1 (P-loop containing nucleoside triphosphate hydrolases superfamily protein )

HSP 1 Score: 248.1 bits (632), Expect = 1.5e-65
Identity = 153/437 (35.01%), Postives = 241/437 (55.15%), Query Frame = 0

Query: 33  LVKKWWRNLQDCFHVYQSLRIPEFNESSQRNLLYWKVSAYLSSLSSLEDSDF-TNLVTGK 92
           L  +++  +Q  FH Y         E  +R+  Y  + +YLS  SS        N   G 
Sbjct: 43  LFGRFYPYIQITFHEYS-------GEHFKRSEAYLGIQSYLSKDSSARAKKLKANTTKGS 102

Query: 93  KPNDILLRLDSNQTVVDEFLGARLLWTNEENGS--------------RNFVLKIRRADKR 152
           K   I+L +D  + + D+F G R+ W +++ G+              R ++L+  R D+ 
Sbjct: 103 K--SIVLSMDDKEEITDDFEGIRVWWQSKKEGATRQSFSFYPEANEKRYYMLRFHRRDRE 162

Query: 153 RILRPYLQHIHALTADEIEHNKGDLRLFMNSKFNNQSH---TRWKSIQFNHPSTFESISM 212
            I+  YL+H+       IE    + +L+ N+    QSH   ++W  + F HP+TF++++M
Sbjct: 163 VIIERYLEHV-MREGKTIEQKNRERKLYSNTP--GQSHGNNSKWSHVTFEHPATFDTLAM 222

Query: 213 EPDLKEKVKSDLESFLKSKQYYHKLGRVWKRSYLLYGPSGTGKSSFVAAMANFLSYDVYD 272
           E + KE++KSDL  F KSK YY K+G+ WKR YLL+GP GTGKS+ +AAMANFL YDVYD
Sbjct: 223 EENKKEEIKSDLIKFSKSKDYYKKIGKAWKRGYLLFGPPGTGKSTMIAAMANFLEYDVYD 282

Query: 273 IDLFKVSDESDLKLLLLQTTSKSIIVVEDLDRFL---------------------IE--- 332
           ++L  V D + L+ LL++T++KSIIV+ED+D  L                     IE   
Sbjct: 283 LELTTVKDNTHLRRLLIETSAKSIIVIEDIDCSLNLTGQRKKKEEEEEDGDDKNTIEKKM 342

Query: 333 --------KSPALSLSALLNFMDGILTSCCAEERMMVFTVNCKEQVDPAILRPGRIDVHI 392
                   K   ++LS LLNF+DG L S C  ER++VFT N  +++DPA++R GR+D HI
Sbjct: 343 MMKNEGENKESKVTLSGLLNFIDG-LWSACGGERIIVFTTNFVDKLDPALIRKGRMDKHI 402

Query: 393 HFPLCDFSAFKNLAINYLGVKDHKLFPQVEEIFQT-GASLSPAEIGELMI--ANRSSPSR 417
               C F AFK LA NYL V++ ++F +++ + +     ++PA++GE ++  + +     
Sbjct: 403 EMSYCCFEAFKVLAKNYLDVEESEMFEEIKRLLEVEEIKMTPADVGENLLPKSEKEGGET 462

BLAST of Moc05g04850 vs. TAIR 10
Match: AT5G40010.1 (AAA-ATPase 1 )

HSP 1 Score: 244.6 bits (623), Expect = 1.6e-64
Identity = 158/453 (34.88%), Postives = 247/453 (54.53%), Query Frame = 0

Query: 41  LQDCFHVYQSLRIPEFNESSQRNLLYWKVSAYLSSLSSLEDSDFT-NLVTGKKPNDILLR 100
           +Q  FH Y         E  +R+ +Y  + +YLS  SS      T N + G K   I+L 
Sbjct: 52  IQITFHEYS-------GERFKRSDVYDAIQSYLSKDSSSRAKKLTANTIKGNK--SIILS 111

Query: 101 LDSNQTVVDEFLGARLLWTNEE--------------NGSRNFVLKIRRADKRRILRPYLQ 160
           +D ++ + DEF G ++ W +++              + SR ++LK  R D+  I + YL 
Sbjct: 112 MDDHEEITDEFQGVKVWWQSKKHQSESRAISFYPKADESRFYMLKFHRRDREVITKKYLN 171

Query: 161 HIHALTADEIEHNKGDLRLFMNSKFNNQS---HTRWKSIQFNHPSTFESISMEPDLKEKV 220
           H+ +     IE    + +L+ N+   N S    T+W  + F HP+TF++++ME   KE++
Sbjct: 172 HVIS-EGKTIEVKNRERKLYSNNPSQNWSGYKQTKWSHVTFEHPATFDTLAMEYKKKEEI 231

Query: 221 KSDLESFLKSKQYYHKLGRVWKRSYLLYGPSGTGKSSFVAAMANFLSYDVYDIDLFKVSD 280
           K+DL  F  SK YY K+G+ WKR YLL+GP GTGKS+ +AAMAN L YDVYD++L  V D
Sbjct: 232 KNDLIKFSNSKDYYKKIGKAWKRGYLLFGPPGTGKSTMIAAMANLLEYDVYDLELTTVKD 291

Query: 281 ESDLKLLLLQTTSKSIIVVEDLDRFL---------------------IE----------K 340
            ++L+ LL++T+ KSIIV+ED+D  L                     IE          K
Sbjct: 292 NTELRRLLIETSGKSIIVIEDIDCSLDLTGQRKQKKDEEEDEDETSPIEKQMKKDQGENK 351

Query: 341 SPALSLSALLNFMDGILTSCCAEERMMVFTVNCKEQVDPAILRPGRIDVHIHFPLCDFSA 400
              ++LS LLNF+DG L S C  ER++VFT N  +++DPA++R GR+D HI    C F A
Sbjct: 352 GSKVTLSGLLNFIDG-LWSACGGERIIVFTTNFIDKLDPALIRKGRMDKHIEMSYCGFEA 411

Query: 401 FKNLAINYLGVK---DHKLFPQVEEIFQT-GASLSPAEIGELMIANRSSPSRAI--KSVI 439
           FK LA NYL  K   D++LF +++ + +     ++PA++GE ++      ++ I  K +I
Sbjct: 412 FKVLANNYLDAKEEDDNELFDEIKRLLEVEEIKMTPADVGENLLKKSEVETKEICLKRLI 471

BLAST of Moc05g04850 vs. TAIR 10
Match: AT5G57480.1 (P-loop containing nucleoside triphosphate hydrolases superfamily protein )

HSP 1 Score: 238.8 bits (608), Expect = 9.0e-63
Identity = 163/472 (34.53%), Postives = 247/472 (52.33%), Query Frame = 0

Query: 1   MELFFRLFCLILIGICFTWLLRIVLFRTGLIFLVKKWWRNLQDCFHVYQSLRIPEFNESS 60
           M+ ++     +L  + F   L   +F   L F   K++  +   F  Y    I E  +  
Sbjct: 1   MKEYWTSLASLLGVLAFCQSLMQSIFPPELRFAFLKFFNRIFHVFSSYCYFDITEI-DGV 60

Query: 61  QRNLLYWKVSAYLSSLSSLEDSDFTNLVTGKKPNDILLRLDSNQTVVDEFLGARLLWTNE 120
             N LY  V  YLSS  S+  +   +L      + I   L +N ++VD F G  +LW + 
Sbjct: 61  NTNELYNAVQLYLSSSVSIAGNRL-SLTRAVNSSSITFGLSNNDSIVDTFNGVTVLWEHV 120

Query: 121 --------------ENGSRNFVLKIRRADKRRILRPYLQHIHALTADEIEHNKGDLRLFM 180
                             R F L+I++ DK  IL  YL +I    A+EI     D  L+ 
Sbjct: 121 VTQRQTQTFAWRPLPEEKRGFTLRIKKKDKTLILNSYLDYIME-RANEIRRKNQDRLLYT 180

Query: 181 NSK---FNNQSHTRWKSIQFNHPSTFESISMEPDLKEKVKSDLESFLKSKQYYHKLGRVW 240
           NS+    +++ H  W+S+ F HPSTFE+++M+P  K+++  DL+ F + + +Y K GR W
Sbjct: 181 NSRGGSLDSRGHP-WESVPFKHPSTFETLAMDPRKKQQIMDDLKDFAEGQVFYQKTGRAW 240

Query: 241 KRSYLLYGPSGTGKSSFVAAMANFLSYDVYDIDLFKVSDESDLKLLLLQTTSKSIIVVED 300
           KR YLLYGP GTGKSS +AAMAN+L YD+YD++L +V   S+L+ LL++T+SKSIIV+ED
Sbjct: 241 KRGYLLYGPPGTGKSSMIAAMANYLGYDIYDLELTEVHSNSELRKLLMKTSSKSIIVIED 300

Query: 301 LDRFLI-------------------------------------EKSPALSLSALLNFMDG 360
           +D  +                                           ++LS LLNF DG
Sbjct: 301 IDCSINLTNRKKNSSNVSSQRSYYDAETRNGSGSGSGGSGEEGGNGNTITLSGLLNFTDG 360

Query: 361 ILTSCCAEERMMVFTVNCKEQVDPAILRPGRIDVHIHFPLCDFSAFKNLAINYL--GVKD 415
            L SCC  ER+ VFT N  E++DPA+LR GR+D+HI+   C+F + K L  NYL  GV+D
Sbjct: 361 -LWSCCGSERIFVFTTNHIEKLDPALLRSGRMDMHIYMSFCNFPSLKILLKNYLGYGVED 420

BLAST of Moc05g04850 vs. TAIR 10
Match: AT4G30250.1 (P-loop containing nucleoside triphosphate hydrolases superfamily protein )

HSP 1 Score: 237.3 bits (604), Expect = 2.6e-62
Identity = 159/464 (34.27%), Postives = 247/464 (53.23%), Query Frame = 0

Query: 8   FCLILIGICFTWLLRIVLFRTGLIFLVKKWWRNLQDCFHVYQSLRIPEFNESSQRNLLYW 67
           FC  ++ + F   LR+      L FL +     ++  F  +    I E  +    N LY 
Sbjct: 17  FCQTIVQLVFPPELRLAF----LHFLTR-----IRHVFSSHIYFDITEI-DGVNTNELYN 76

Query: 68  KVSAYLSSLSSLED-----SDFTNLVTGKKPN--DILLRLDSNQTVVDEFLGARLLWTN- 127
            V  YLSS  ++ D     ++ T L   + PN   +   L +N  + D F G  +LW + 
Sbjct: 77  AVQLYLSSSVTVNDAVSSSNNNTRLSLTRVPNSSSVTFGLSNNDRITDVFNGVTILWEHV 136

Query: 128 -------------EENGSRNFVLKIRRADKRRILRPYLQHIHALTADEIEHNKGDLRLFM 187
                             R F L+I + DK  +L  YL +I    ++EI     +  L+ 
Sbjct: 137 VVQRQVQSFSWRPMPEEKRGFTLQINKRDKALVLDSYLDYIVG-KSEEIRRRNEERLLYT 196

Query: 188 NSK---FNNQSHTRWKSIQFNHPSTFESISMEPDLKEKVKSDLESFLKSKQYYHKLGRVW 247
           NS+    + +SH  W S++F HPSTF++++M+P+ K+++  DL  F   + +Y K GR W
Sbjct: 197 NSRGVSLDARSHP-WDSVRFKHPSTFDTLAMDPEKKKRIMEDLREFANGQGFYQKTGRAW 256

Query: 248 KRSYLLYGPSGTGKSSFVAAMANFLSYDVYDIDLFKVSDESDLKLLLLQTTSKSIIVVED 307
           KR YLLYGP GTGKSS +AAMAN+L YD+YD++L +V + S+L+ LL++T+SKSIIV+ED
Sbjct: 257 KRGYLLYGPPGTGKSSLIAAMANYLGYDIYDLELTEVQNNSELRKLLMKTSSKSIIVIED 316

Query: 308 LD---------------------------RFLIEKSPALSLSALLNFMDGILTSCCAEER 367
           +D                             L E   +++LS LLNF DG L SCC  E+
Sbjct: 317 IDCSISLTKRGKNKKKNGSYEYDPGLTNGSGLEEPGSSVTLSGLLNFTDG-LWSCCGSEK 376

Query: 368 MMVFTVNCKEQVDPAILRPGRIDVHIHFPLCDFSAFKNLAINYLGVK----DHKLFPQVE 417
           + VFT N  E++D A++R GR+D+H+H   C F A K L  NYL ++    D  +  ++E
Sbjct: 377 IFVFTTNHIEKLDSALMRSGRMDMHVHMGFCKFPALKILLKNYLRLEEEDMDSVVLKEME 436

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038903225.12.8e-23988.13AAA-ATPase At2g46620 [Benincasa hispida][more]
XP_008437854.14.4e-23787.08PREDICTED: AAA-ATPase At2g46620 [Cucumis melo][more]
XP_004133798.12.9e-23687.08AAA-ATPase At2g46620 [Cucumis sativus][more]
XP_023527367.11.9e-23285.63AAA-ATPase At2g46620-like [Cucurbita pepo subsp. pepo][more]
XP_022924392.14.0e-23085.21AAA-ATPase At2g46620-like [Cucurbita moschata] >KAG6582688.1 AAA-ATPase, partial... [more]
Match NameE-valueIdentityDescription
F4IJ772.1e-16563.56AAA-ATPase At2g46620 OS=Arabidopsis thaliana OX=3702 GN=At2g46620 PE=2 SV=1[more]
Q9LJJ72.1e-6435.01AAA-ATPase At3g28580 OS=Arabidopsis thaliana OX=3702 GN=At3g28580 PE=2 SV=1[more]
Q9FLD52.3e-6334.88AAA-ATPase ASD, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=AATP1 PE=1 SV=1[more]
Q9FKM31.3e-6134.53AAA-ATPase At5g57480 OS=Arabidopsis thaliana OX=3702 GN=At5g57480 PE=3 SV=1[more]
F4JPK83.7e-6134.27AAA-ATPase At4g30250 OS=Arabidopsis thaliana OX=3702 GN=At4g30250 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
A0A1S3AVK02.1e-23787.08AAA-ATPase At2g46620 OS=Cucumis melo OX=3656 GN=LOC103483161 PE=3 SV=1[more]
A0A0A0L5T81.4e-23687.08AAA domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G119330 PE=3 SV... [more]
A0A6J1E8T11.9e-23085.21AAA-ATPase At2g46620-like OS=Cucurbita moschata OX=3662 GN=LOC111431901 PE=3 SV=... [more]
A0A6J1IWA65.6e-23084.79AAA-ATPase At2g46620 OS=Cucurbita maxima OX=3661 GN=LOC111479067 PE=3 SV=1[more]
A0A5A7U3P91.2e-22787.31AAA-ATPase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold291G00800 PE=... [more]
Match NameE-valueIdentityDescription
AT2G46620.11.5e-16663.56P-loop containing nucleoside triphosphate hydrolases superfamily protein [more]
AT3G28580.11.5e-6535.01P-loop containing nucleoside triphosphate hydrolases superfamily protein [more]
AT5G40010.11.6e-6434.88AAA-ATPase 1 [more]
AT5G57480.19.0e-6334.53P-loop containing nucleoside triphosphate hydrolases superfamily protein [more]
AT4G30250.12.6e-6234.27P-loop containing nucleoside triphosphate hydrolases superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (OHB3-1) v2
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR003593AAA+ ATPase domainSMARTSM00382AAA_5coord: 223..348
e-value: 3.0E-5
score: 33.5
IPR025753AAA-type ATPase, N-terminal domainPFAMPF14363AAA_assoccoord: 33..119
e-value: 1.7E-11
score: 44.3
NoneNo IPR availableGENE3D6.10.280.40coord: 344..406
e-value: 3.9E-6
score: 28.7
NoneNo IPR availablePANTHERPTHR23070BCS1 AAA-TYPE ATPASEcoord: 8..476
NoneNo IPR availablePANTHERPTHR23070:SF176BNAC04G00800D PROTEINcoord: 8..476
NoneNo IPR availableCDDcd00009AAAcoord: 224..346
e-value: 4.99357E-10
score: 56.0003
IPR027417P-loop containing nucleoside triphosphate hydrolaseGENE3D3.40.50.300coord: 176..343
e-value: 8.9E-34
score: 118.8
IPR027417P-loop containing nucleoside triphosphate hydrolaseSUPERFAMILY52540P-loop containing nucleoside triphosphate hydrolasescoord: 161..407
IPR003959ATPase, AAA-type, corePFAMPF00004AAAcoord: 228..346
e-value: 8.3E-13
score: 48.9

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Moc05g04850.1Moc05g04850.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0005524 ATP binding
molecular_function GO:0016887 ATP hydrolysis activity