Moc05g04160 (gene) Bitter gourd (OHB3-1) v2

Overview
NameMoc05g04160
Typegene
OrganismMomordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
DescriptionAB hydrolase-1 domain-containing protein
Locationchr5: 2898013 .. 2901626 (+)
RNA-Seq ExpressionMoc05g04160
SyntenyMoc05g04160
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCCACCGAGACCAATCCCATTAATGCAGCATCGCCGCCGGATCACGCGACCGGGAAATGGTACTCCGTGCCGGAGCTCCGGCTCCGCGACCACCACTTCACTGTGCCTCTCGATTACTCTCTGGATCAGGATGCTTCTCCTAAGATCTCCGTTTTTGCGCGGGAAGTTGTTTTGGGTAAATTAATCAATCAGTAATCAAGCCTTAATTCGACTTTGATATTACTTTCGACATTTTGATTAATCCATTTGTTTTTTAATTTTGGTTGCTCCTTTCTTATTTATTTGAATGATCCAGGACCAATTATGGAGCATCGATGGTTGAAACTTAGAATTTCGTAGTATAGAGGTGACTAGTGTTGATCACTAGCAGAGTTTTCTCCATTCATTTATTAATTTTGTGGAGTTATGGTGTCAGGGAAGTTATAGATTGGTGTCATTGATGTTAATAGGAATTATGACACAACAACGAAATCAATTAATGGGCATCTAATAGAACAAACTTTGTGTTGCTTAGATAATTTTTTCCTTCATTAATGAACACTTCAACCACATGAATCAATTAACTTAATGGTATGCATGAAATCGTAATGAGAAAAGAACGAGAGTAAGAAAAAAAATATATGATTAGATGTGCTTTTAACATCTTTTGAAATCTCTTTAAATTATGATTAGTTTGTGTTGCTTTATTTGTAAATCTATACCCATTTCTACTGTGATATTCAGTCATACTTTTGTATGAATGACTATTATTTGAAAATTATGGATGCACTCATTGAGTAATAGTGGGGACTTTTATGCGTATGGGCTCTGAAAATATGTGGACATCTTAGTCAGAGTACAAATAATTATCAGGAATTGTGTAGAAGTGTTGAGTATTGCTGAAGCTACTGATTCAATGATTGAGGGTCTCCTTAAATATTGAATGATCTTATTTTAACTTAGATTTATCAGGAATTGTGTAGAATTTCTTTGATAATGATTAGCAATCATCCCTTATTTGAACTTAGATTTATTGTGCCAGTTTTTACATGTACATGGATTGTTGTATGATTTTAGCACTAGTTATCCTTAACATATGCAGAATGTGCATTGCAGTGGGGAAAGAAGAGCAGTCGATGCCATATCTTTTATACTTACAAGGTGGACCGGGATTTGAGAGTCCCCGACCGACCGAAGCAAGTGGATGGATGCAAAAAGCGTGTGAAGAATTTCGTGTTGTGTTGATGGATCAGGCATGACTTTTTGTTGACTGCCTTTGGAAAAAGGCATTTGAATTTTGTAATATAAACAACTGTCTTCTTGATTGTTGATCCCGCATATATATACGTGTCATTGCAGCGAGGAACAGGATTATCAACTCCTCTGACTTCATCGTCTATGTCGCAATTTCAAAGTGCAGAGGACTTAGCCAACTACTTGAAACATTTTCGAGCTGACAACATCGTGAATGATGCTGAATTTATTAGGACTCGTCTTGTTCCTGATGCTGGACCTTGGACTATTTTGGGTCAGGTATTTAAATCCACTGTTTATTTTTTAGTTTCGAAATTTTGCTACCAACATGTTGAGCTTACTACTATTTTCTCAAGCATGTTGAAGTTATATAAAGCAAATAGCAGACTTTTTGGCTTTGAGATTTTTGAATGTGGCTCTGCTTTTGTTGTATGGGGTAATTTACACCACTACAAGTTCAAGGAAATTCTCTGTTTCCCTGTGCAGAGCTTTGGTGGTTTTTGTGCAGTTACTTATTTGAGTTTTGCACCACAAGGATTGAAACAAGTCCTCATAACTGGAGGAATCCCTCCAATTGGGAATAAATGCACTGCGGATTCTGTATATAGAGCATGCTTTGAAAAGGTTATAATTCAAAACGAAAAGTACTACAAGCGGTATCCTCAGGATGTTGAAATCATCCGTGAAGTTGCGAAATACTTGGCTGAGCATGGTGGCGGGGTGAGGACAGTTGTCTTAAAACTCGAACTAATTGCTTAAAATTCTCTCAGTTCCCTTAGCCATTCCCTGAAAAACCACCCTTTTACCTTCTGTTTTTGTTTAGGTTGTTCTTCCCTCTGGTGGTATCCTAACCCCTAAGGGGCTGCAAGTTCTTGGTCTTTTTGCTTTAGGATCTAGTACAGGTTTTGAGCGCTTGCATTATCTGTAAGGCCTCTTATTATTATTATTATTATTTTTTCTTTTTTCCTTTCTGGTCTCTATCATTTGGACCAGTGAATGGCCACCCAAGATACTGTAATCTAATTACTTTATATTTCCCACCTCAATTATGGCAGCTTTGAGAGAGTTTGGGATCCTGTAATAGTTCCTGGAGCGCCAAAACGAATCAGTTCTTTCTTCCTCCGCGCTGTTAGTATTCTACAGTTTCTATTGCTATTGAATATAACTATAGCTTCATTCTTGAATGAATCTAAATGATTAGACATATTTGCAGTGTGATAACTGGCTCTCACTTGATTCAAATCCTCTATATGTTCTTCTACATGAATCAATATATTGCCAGGTAGCTTTCTTCTTTTTCCTTTTGATTCTCAGAGTAGAACTATGTTTTGGCCCGTCAGAACTTTTTGTAGTGTTAACTTTTTGCCACTTCCTGTGCCGGTTTCCCGAAGGGTGCCTCATCTCGGTGGTCTGCTCAAAGAATAAAGAATGAACTTAAGAATAAGTTCGATGCAAATAACGCCCTGAAAGAAGGATGTCCCATGTTTTTCACAGGCGAGGTAACTGCACTATGCTCAACTGGTTAAAACACTTATATTTGAGTAGAAGGTCGTGAGTTTGAATCTCCATCTAAGTTTATATTTGAGTAGAAGGTCGTGAGTTTGAATCTCCATCTAAGTTTATAAGGCTATGCTTGACTTTGGGAATTTAAGCACAAAGATATAAAACTCCCATTGTGTATGCTCCATTAATTTGTTACACTTCTGCAGATGGTCTTCCCGTGGATGTTTGACGAGATTCATGCCTTGAGACCATTGAAAGATGCTGCTCGTATATTGGCCGAGAAAGAGGATTGGCCTCCACTATATGACGTTGCCGCTCTTAGAAACAACAAGGTATTCTGTTTCAAGAGCTTCTGCATTTCCATGTTAGCAAAAATGAGCTTGTCTTTCTCCTACATCTTCTGAATGAGTGCGTAAAAATGATCATATTGATAACGTTTTTAACCCACAAAATCTCGCACAACGTTTCATAGAAGCACGTCCATCCGTCACGGGTAGCCTAGTGGCCACTTACTTCTACAAGTTTTCTTGACAACCAAATGTAGTAGAATCAAGTAGTCGTCCTAAGAGACTACTCGAGGTACTTGCAAGTTTGCTAGATAGATATTCGAGGGAAAAAAACCAGGATCAGCTTGGGACTCGTCAACGAAAATTAACATGTTTTCTGGATTCCCCTCACAGGTTCCAGTCGCAGCTGCTGTTTATTACGAAGATATGTTTGTAAACTTCAAGCTGGCCATGGAGACAGCTTCCCAGATAGCAGGAATAAGGCTGTGGATAACCAATGAATATATGCATTCTGGTCTGCGAGATGCAGGGCCCCAAGTTCTGGATCACTTGATGGGATTGTTAAATGGAAAGAAGCCTTTATTCTGA

mRNA sequence

ATGGCCACCGAGACCAATCCCATTAATGCAGCATCGCCGCCGGATCACGCGACCGGGAAATGGTACTCCGTGCCGGAGCTCCGGCTCCGCGACCACCACTTCACTGTGCCTCTCGATTACTCTCTGGATCAGGATGCTTCTCCTAAGATCTCCGTTTTTGCGCGGGAAGTTGTTTTGGTGGGGAAAGAAGAGCAGTCGATGCCATATCTTTTATACTTACAAGGTGGACCGGGATTTGAGAGTCCCCGACCGACCGAAGCAAGTGGATGGATGCAAAAAGCGTGTGAAGAATTTCGTGTTGTGTTGATGGATCAGCGAGGAACAGGATTATCAACTCCTCTGACTTCATCGTCTATGTCGCAATTTCAAAGTGCAGAGGACTTAGCCAACTACTTGAAACATTTTCGAGCTGACAACATCGTGAATGATGCTGAATTTATTAGGACTCGTCTTGTTCCTGATGCTGGACCTTGGACTATTTTGGGTCAGAGCTTTGGTGGTTTTTGTGCAGTTACTTATTTGAGTTTTGCACCACAAGGATTGAAACAAGTCCTCATAACTGGAGGAATCCCTCCAATTGGGAATAAATGCACTGCGGATTCTGTATATAGAGCATGCTTTGAAAAGGTTATAATTCAAAACGAAAAGTACTACAAGCGGTATCCTCAGGATGTTGAAATCATCCGTGAAGTTGCGAAATACTTGGCTGAGCATGGTGGCGGGGTTGTTCTTCCCTCTGGTGGTATCCTAACCCCTAAGGGGCTGCAAGTTCTTGGTCTTTTTGCTTTAGGATCTAGTACAGGTTTTGAGCGCTTGCATTATCTCTTTGAGAGAGTTTGGGATCCTGTAATAGTTCCTGGAGCGCCAAAACGAATCAGTTCTTTCTTCCTCCGCGCTTGTGATAACTGGCTCTCACTTGATTCAAATCCTCTATATGTTCTTCTACATGAATCAATATATTGCCAGGGTGCCTCATCTCGGTGGTCTGCTCAAAGAATAAAGAATGAACTTAAGAATAAGTTCGATGCAAATAACGCCCTGAAAGAAGGATGTCCCATGTTTTTCACAGGCGAGATGGTCTTCCCGTGGATGTTTGACGAGATTCATGCCTTGAGACCATTGAAAGATGCTGCTCGTATATTGGCCGAGAAAGAGGATTGGCCTCCACTATATGACGTTGCCGCTCTTAGAAACAACAAGGTTCCAGTCGCAGCTGCTGTTTATTACGAAGATATGTTTGTAAACTTCAAGCTGGCCATGGAGACAGCTTCCCAGATAGCAGGAATAAGGCTGTGGATAACCAATGAATATATGCATTCTGGTCTGCGAGATGCAGGGCCCCAAGTTCTGGATCACTTGATGGGATTGTTAAATGGAAAGAAGCCTTTATTCTGA

Coding sequence (CDS)

ATGGCCACCGAGACCAATCCCATTAATGCAGCATCGCCGCCGGATCACGCGACCGGGAAATGGTACTCCGTGCCGGAGCTCCGGCTCCGCGACCACCACTTCACTGTGCCTCTCGATTACTCTCTGGATCAGGATGCTTCTCCTAAGATCTCCGTTTTTGCGCGGGAAGTTGTTTTGGTGGGGAAAGAAGAGCAGTCGATGCCATATCTTTTATACTTACAAGGTGGACCGGGATTTGAGAGTCCCCGACCGACCGAAGCAAGTGGATGGATGCAAAAAGCGTGTGAAGAATTTCGTGTTGTGTTGATGGATCAGCGAGGAACAGGATTATCAACTCCTCTGACTTCATCGTCTATGTCGCAATTTCAAAGTGCAGAGGACTTAGCCAACTACTTGAAACATTTTCGAGCTGACAACATCGTGAATGATGCTGAATTTATTAGGACTCGTCTTGTTCCTGATGCTGGACCTTGGACTATTTTGGGTCAGAGCTTTGGTGGTTTTTGTGCAGTTACTTATTTGAGTTTTGCACCACAAGGATTGAAACAAGTCCTCATAACTGGAGGAATCCCTCCAATTGGGAATAAATGCACTGCGGATTCTGTATATAGAGCATGCTTTGAAAAGGTTATAATTCAAAACGAAAAGTACTACAAGCGGTATCCTCAGGATGTTGAAATCATCCGTGAAGTTGCGAAATACTTGGCTGAGCATGGTGGCGGGGTTGTTCTTCCCTCTGGTGGTATCCTAACCCCTAAGGGGCTGCAAGTTCTTGGTCTTTTTGCTTTAGGATCTAGTACAGGTTTTGAGCGCTTGCATTATCTCTTTGAGAGAGTTTGGGATCCTGTAATAGTTCCTGGAGCGCCAAAACGAATCAGTTCTTTCTTCCTCCGCGCTTGTGATAACTGGCTCTCACTTGATTCAAATCCTCTATATGTTCTTCTACATGAATCAATATATTGCCAGGGTGCCTCATCTCGGTGGTCTGCTCAAAGAATAAAGAATGAACTTAAGAATAAGTTCGATGCAAATAACGCCCTGAAAGAAGGATGTCCCATGTTTTTCACAGGCGAGATGGTCTTCCCGTGGATGTTTGACGAGATTCATGCCTTGAGACCATTGAAAGATGCTGCTCGTATATTGGCCGAGAAAGAGGATTGGCCTCCACTATATGACGTTGCCGCTCTTAGAAACAACAAGGTTCCAGTCGCAGCTGCTGTTTATTACGAAGATATGTTTGTAAACTTCAAGCTGGCCATGGAGACAGCTTCCCAGATAGCAGGAATAAGGCTGTGGATAACCAATGAATATATGCATTCTGGTCTGCGAGATGCAGGGCCCCAAGTTCTGGATCACTTGATGGGATTGTTAAATGGAAAGAAGCCTTTATTCTGA

Protein sequence

MATETNPINAASPPDHATGKWYSVPELRLRDHHFTVPLDYSLDQDASPKISVFAREVVLVGKEEQSMPYLLYLQGGPGFESPRPTEASGWMQKACEEFRVVLMDQRGTGLSTPLTSSSMSQFQSAEDLANYLKHFRADNIVNDAEFIRTRLVPDAGPWTILGQSFGGFCAVTYLSFAPQGLKQVLITGGIPPIGNKCTADSVYRACFEKVIIQNEKYYKRYPQDVEIIREVAKYLAEHGGGVVLPSGGILTPKGLQVLGLFALGSSTGFERLHYLFERVWDPVIVPGAPKRISSFFLRACDNWLSLDSNPLYVLLHESIYCQGASSRWSAQRIKNELKNKFDANNALKEGCPMFFTGEMVFPWMFDEIHALRPLKDAARILAEKEDWPPLYDVAALRNNKVPVAAAVYYEDMFVNFKLAMETASQIAGIRLWITNEYMHSGLRDAGPQVLDHLMGLLNGKKPLF
Homology
BLAST of Moc05g04160 vs. NCBI nr
Match: XP_022147514.1 (uncharacterized protein LOC111016418 [Momordica charantia])

HSP 1 Score: 966.1 bits (2496), Expect = 1.1e-277
Identity = 464/464 (100.00%), Postives = 464/464 (100.00%), Query Frame = 0

Query: 1   MATETNPINAASPPDHATGKWYSVPELRLRDHHFTVPLDYSLDQDASPKISVFAREVVLV 60
           MATETNPINAASPPDHATGKWYSVPELRLRDHHFTVPLDYSLDQDASPKISVFAREVVLV
Sbjct: 54  MATETNPINAASPPDHATGKWYSVPELRLRDHHFTVPLDYSLDQDASPKISVFAREVVLV 113

Query: 61  GKEEQSMPYLLYLQGGPGFESPRPTEASGWMQKACEEFRVVLMDQRGTGLSTPLTSSSMS 120
           GKEEQSMPYLLYLQGGPGFESPRPTEASGWMQKACEEFRVVLMDQRGTGLSTPLTSSSMS
Sbjct: 114 GKEEQSMPYLLYLQGGPGFESPRPTEASGWMQKACEEFRVVLMDQRGTGLSTPLTSSSMS 173

Query: 121 QFQSAEDLANYLKHFRADNIVNDAEFIRTRLVPDAGPWTILGQSFGGFCAVTYLSFAPQG 180
           QFQSAEDLANYLKHFRADNIVNDAEFIRTRLVPDAGPWTILGQSFGGFCAVTYLSFAPQG
Sbjct: 174 QFQSAEDLANYLKHFRADNIVNDAEFIRTRLVPDAGPWTILGQSFGGFCAVTYLSFAPQG 233

Query: 181 LKQVLITGGIPPIGNKCTADSVYRACFEKVIIQNEKYYKRYPQDVEIIREVAKYLAEHGG 240
           LKQVLITGGIPPIGNKCTADSVYRACFEKVIIQNEKYYKRYPQDVEIIREVAKYLAEHGG
Sbjct: 234 LKQVLITGGIPPIGNKCTADSVYRACFEKVIIQNEKYYKRYPQDVEIIREVAKYLAEHGG 293

Query: 241 GVVLPSGGILTPKGLQVLGLFALGSSTGFERLHYLFERVWDPVIVPGAPKRISSFFLRAC 300
           GVVLPSGGILTPKGLQVLGLFALGSSTGFERLHYLFERVWDPVIVPGAPKRISSFFLRAC
Sbjct: 294 GVVLPSGGILTPKGLQVLGLFALGSSTGFERLHYLFERVWDPVIVPGAPKRISSFFLRAC 353

Query: 301 DNWLSLDSNPLYVLLHESIYCQGASSRWSAQRIKNELKNKFDANNALKEGCPMFFTGEMV 360
           DNWLSLDSNPLYVLLHESIYCQGASSRWSAQRIKNELKNKFDANNALKEGCPMFFTGEMV
Sbjct: 354 DNWLSLDSNPLYVLLHESIYCQGASSRWSAQRIKNELKNKFDANNALKEGCPMFFTGEMV 413

Query: 361 FPWMFDEIHALRPLKDAARILAEKEDWPPLYDVAALRNNKVPVAAAVYYEDMFVNFKLAM 420
           FPWMFDEIHALRPLKDAARILAEKEDWPPLYDVAALRNNKVPVAAAVYYEDMFVNFKLAM
Sbjct: 414 FPWMFDEIHALRPLKDAARILAEKEDWPPLYDVAALRNNKVPVAAAVYYEDMFVNFKLAM 473

Query: 421 ETASQIAGIRLWITNEYMHSGLRDAGPQVLDHLMGLLNGKKPLF 465
           ETASQIAGIRLWITNEYMHSGLRDAGPQVLDHLMGLLNGKKPLF
Sbjct: 474 ETASQIAGIRLWITNEYMHSGLRDAGPQVLDHLMGLLNGKKPLF 517

BLAST of Moc05g04160 vs. NCBI nr
Match: XP_008437982.1 (PREDICTED: proline iminopeptidase [Cucumis melo] >TYK17630.1 proline iminopeptidase [Cucumis melo var. makuwa])

HSP 1 Score: 862.4 bits (2227), Expect = 1.7e-246
Identity = 404/455 (88.79%), Postives = 432/455 (94.95%), Query Frame = 0

Query: 10  AASPPDHATGKWYSVPELRLRDHHFTVPLDYSLDQDASPKISVFAREVVLVGKEEQSMPY 69
           A SPP H  G WYSVPELRLRDHHF+VPL+YSLDQ +S +ISVFAREVV VGKE+Q MPY
Sbjct: 55  APSPPVHVAGTWYSVPELRLRDHHFSVPLNYSLDQGSSTRISVFAREVVSVGKEDQPMPY 114

Query: 70  LLYLQGGPGFESPRPTEASGWMQKACEEFRVVLMDQRGTGLSTPLTSSSMSQFQSAEDLA 129
           LLYLQGGPGFE  RP+EASGW+QKACEEFRV+LMDQRGTGLSTPLT SSMSQF+SAEDLA
Sbjct: 115 LLYLQGGPGFECARPSEASGWIQKACEEFRVILMDQRGTGLSTPLTPSSMSQFRSAEDLA 174

Query: 130 NYLKHFRADNIVNDAEFIRTRLVPDAGPWTILGQSFGGFCAVTYLSFAPQGLKQVLITGG 189
           NYLKHFRADNIVNDAEFIRTRLVPDA PWTILGQS+GGFCAVTYLSFAPQGLKQVLITGG
Sbjct: 175 NYLKHFRADNIVNDAEFIRTRLVPDAAPWTILGQSYGGFCAVTYLSFAPQGLKQVLITGG 234

Query: 190 IPPIGNKCTADSVYRACFEKVIIQNEKYYKRYPQDVEIIREVAKYLAEHGGGVVLPSGGI 249
           IPPIGN CTADSVYRACFEKVIIQNEKYYKRYPQD+EI+REV KYLA++GGGV+LPSGGI
Sbjct: 235 IPPIGNGCTADSVYRACFEKVIIQNEKYYKRYPQDIEIVREVVKYLADNGGGVLLPSGGI 294

Query: 250 LTPKGLQVLGLFALGSSTGFERLHYLFERVWDPVIVPGAPKRISSFFLRACDNWLSLDSN 309
           LTPKGLQ LGL ALG+STGFERLHYLFERVWDP++VPGAPKRIS FFL A DNWLSLDSN
Sbjct: 295 LTPKGLQTLGLSALGTSTGFERLHYLFERVWDPILVPGAPKRISFFFLNAIDNWLSLDSN 354

Query: 310 PLYVLLHESIYCQGASSRWSAQRIKNELKNKFDANNALKEGCPMFFTGEMVFPWMFDEIH 369
           PLYVLLHESIYCQGASSRWSAQRIKNE++NKFDAN A+KEGCP++FTGEM+FPWMFDEIH
Sbjct: 355 PLYVLLHESIYCQGASSRWSAQRIKNEVENKFDANKAVKEGCPVYFTGEMIFPWMFDEIH 414

Query: 370 ALRPLKDAARILAEKEDWPPLYDVAALRNNKVPVAAAVYYEDMFVNFKLAMETASQIAGI 429
           ALRP KDAA ILA+KEDWPPLYD+AAL+NNKVPVAAAVYYEDMFVNFKLAMETASQIAGI
Sbjct: 415 ALRPFKDAAHILADKEDWPPLYDIAALKNNKVPVAAAVYYEDMFVNFKLAMETASQIAGI 474

Query: 430 RLWITNEYMHSGLRDAGPQVLDHLMGLLNGKKPLF 465
           RLWITNE+MHSGLRDAGPQVLDHLMGLLNGKKPLF
Sbjct: 475 RLWITNEFMHSGLRDAGPQVLDHLMGLLNGKKPLF 509

BLAST of Moc05g04160 vs. NCBI nr
Match: KAA0048938.1 (proline iminopeptidase [Cucumis melo var. makuwa])

HSP 1 Score: 861.3 bits (2224), Expect = 3.8e-246
Identity = 403/455 (88.57%), Postives = 432/455 (94.95%), Query Frame = 0

Query: 10  AASPPDHATGKWYSVPELRLRDHHFTVPLDYSLDQDASPKISVFAREVVLVGKEEQSMPY 69
           A SPP H  G WYSVPELRLRDHHF+VPL+YSLDQ +S +ISVFAREVV VGKE+Q MPY
Sbjct: 9   APSPPVHVAGTWYSVPELRLRDHHFSVPLNYSLDQGSSTRISVFAREVVSVGKEDQPMPY 68

Query: 70  LLYLQGGPGFESPRPTEASGWMQKACEEFRVVLMDQRGTGLSTPLTSSSMSQFQSAEDLA 129
           LLYLQGGPGFE  RP+EASGW+QKAC+EFRV+LMDQRGTGLSTPLT SSMSQF+SAEDLA
Sbjct: 69  LLYLQGGPGFECARPSEASGWIQKACDEFRVILMDQRGTGLSTPLTPSSMSQFRSAEDLA 128

Query: 130 NYLKHFRADNIVNDAEFIRTRLVPDAGPWTILGQSFGGFCAVTYLSFAPQGLKQVLITGG 189
           NYLKHFRADNIVNDAEFIRTRLVPDA PWTILGQS+GGFCAVTYLSFAPQGLKQVLITGG
Sbjct: 129 NYLKHFRADNIVNDAEFIRTRLVPDAAPWTILGQSYGGFCAVTYLSFAPQGLKQVLITGG 188

Query: 190 IPPIGNKCTADSVYRACFEKVIIQNEKYYKRYPQDVEIIREVAKYLAEHGGGVVLPSGGI 249
           IPPIGN CTADSVYRACFEKVIIQNEKYYKRYPQD+EI+REV KYLA++GGGV+LPSGGI
Sbjct: 189 IPPIGNGCTADSVYRACFEKVIIQNEKYYKRYPQDIEIVREVVKYLADNGGGVLLPSGGI 248

Query: 250 LTPKGLQVLGLFALGSSTGFERLHYLFERVWDPVIVPGAPKRISSFFLRACDNWLSLDSN 309
           LTPKGLQ LGL ALG+STGFERLHYLFERVWDP++VPGAPKRIS FFL A DNWLSLDSN
Sbjct: 249 LTPKGLQTLGLSALGTSTGFERLHYLFERVWDPILVPGAPKRISFFFLNAIDNWLSLDSN 308

Query: 310 PLYVLLHESIYCQGASSRWSAQRIKNELKNKFDANNALKEGCPMFFTGEMVFPWMFDEIH 369
           PLYVLLHESIYCQGASSRWSAQRIKNE++NKFDAN A+KEGCP++FTGEM+FPWMFDEIH
Sbjct: 309 PLYVLLHESIYCQGASSRWSAQRIKNEVENKFDANKAVKEGCPVYFTGEMIFPWMFDEIH 368

Query: 370 ALRPLKDAARILAEKEDWPPLYDVAALRNNKVPVAAAVYYEDMFVNFKLAMETASQIAGI 429
           ALRP KDAA ILA+KEDWPPLYD+AAL+NNKVPVAAAVYYEDMFVNFKLAMETASQIAGI
Sbjct: 369 ALRPFKDAAHILADKEDWPPLYDIAALKNNKVPVAAAVYYEDMFVNFKLAMETASQIAGI 428

Query: 430 RLWITNEYMHSGLRDAGPQVLDHLMGLLNGKKPLF 465
           RLWITNE+MHSGLRDAGPQVLDHLMGLLNGKKPLF
Sbjct: 429 RLWITNEFMHSGLRDAGPQVLDHLMGLLNGKKPLF 463

BLAST of Moc05g04160 vs. NCBI nr
Match: XP_022933365.1 (uncharacterized protein LOC111440690 [Cucurbita moschata])

HSP 1 Score: 852.0 bits (2200), Expect = 2.3e-243
Identity = 399/460 (86.74%), Postives = 429/460 (93.26%), Query Frame = 0

Query: 5   TNPINAASPPDHATGKWYSVPELRLRDHHFTVPLDYSLDQDASPKISVFAREVVLVGKEE 64
           TNP N ASPP+HA G WYSVPELRLRDH+F+VPL+YSLD  +SPKISV+AREVV VGKEE
Sbjct: 41  TNPSNGASPPEHAAGTWYSVPELRLRDHYFSVPLNYSLDH-SSPKISVYAREVVSVGKEE 100

Query: 65  QSMPYLLYLQGGPGFESPRPTEASGWMQKACEEFRVVLMDQRGTGLSTPLTSSSMSQFQS 124
           Q MPYLLYLQGGPGFE PRPTEASGW+QKACEEFRV+LMDQRGTGLSTPL+ SSMSQFQS
Sbjct: 101 QPMPYLLYLQGGPGFECPRPTEASGWIQKACEEFRVILMDQRGTGLSTPLSPSSMSQFQS 160

Query: 125 AEDLANYLKHFRADNIVNDAEFIRTRLVPDAGPWTILGQSFGGFCAVTYLSFAPQGLKQV 184
           AEDLA+YLKHFRADNIVNDAEFIRTRLVPDA PWTILGQS+GGFCAVTYLSFAPQGLKQV
Sbjct: 161 AEDLADYLKHFRADNIVNDAEFIRTRLVPDAAPWTILGQSYGGFCAVTYLSFAPQGLKQV 220

Query: 185 LITGGIPPIGNKCTADSVYRACFEKVIIQNEKYYKRYPQDVEIIREVAKYLAEHGGGVVL 244
           LITGGIPPIGN CTADSVYRACFEK+IIQNEKYYKRYPQDV+I+ EV KYL E+GGG+ L
Sbjct: 221 LITGGIPPIGNGCTADSVYRACFEKIIIQNEKYYKRYPQDVKIVHEVVKYLEENGGGIPL 280

Query: 245 PSGGILTPKGLQVLGLFALGSSTGFERLHYLFERVWDPVIVPGAPKRISSFFLRACDNWL 304
           P GGILTPKGLQ LGL ALGSSTGFER+HYLFERVWDP+IVPGAPKRIS FFL A   WL
Sbjct: 281 PCGGILTPKGLQTLGLSALGSSTGFERMHYLFERVWDPIIVPGAPKRISYFFLNAISGWL 340

Query: 305 SLDSNPLYVLLHESIYCQGASSRWSAQRIKNELKNKFDANNALKEGCPMFFTGEMVFPWM 364
           SLDSNPLY L+HESIYCQGASSRWSAQRI NEL+NKFDA  A+KEGCP++FTGEM+FPWM
Sbjct: 341 SLDSNPLYGLMHESIYCQGASSRWSAQRIMNELENKFDATKAVKEGCPVYFTGEMIFPWM 400

Query: 365 FDEIHALRPLKDAARILAEKEDWPPLYDVAALRNNKVPVAAAVYYEDMFVNFKLAMETAS 424
           FDEIHAL+P KDAA ILAEKEDWPPLYD+AAL+NNKVPVAAAVYYEDM+VNFKLAMETAS
Sbjct: 401 FDEIHALKPFKDAANILAEKEDWPPLYDIAALKNNKVPVAAAVYYEDMYVNFKLAMETAS 460

Query: 425 QIAGIRLWITNEYMHSGLRDAGPQVLDHLMGLLNGKKPLF 465
           QIAGIRLW+TNE+MHSGLRD GPQVLDHLMGLLNGKKPLF
Sbjct: 461 QIAGIRLWVTNEFMHSGLRDGGPQVLDHLMGLLNGKKPLF 499

BLAST of Moc05g04160 vs. NCBI nr
Match: KAG6597118.1 (Proline iminopeptidase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 850.5 bits (2196), Expect = 6.8e-243
Identity = 398/460 (86.52%), Postives = 429/460 (93.26%), Query Frame = 0

Query: 5   TNPINAASPPDHATGKWYSVPELRLRDHHFTVPLDYSLDQDASPKISVFAREVVLVGKEE 64
           TNP N ASPP+HA G WYSVPELRLRDH+F+VPL+YSLD  +SPKISV+AREVV VGKEE
Sbjct: 41  TNPSNGASPPEHAAGTWYSVPELRLRDHYFSVPLNYSLDH-SSPKISVYAREVVSVGKEE 100

Query: 65  QSMPYLLYLQGGPGFESPRPTEASGWMQKACEEFRVVLMDQRGTGLSTPLTSSSMSQFQS 124
           Q MPYL+YLQGGPGFE PRPTEASGW+QKACEEFRV+LMDQRGTGLSTPL+ SSMSQFQ+
Sbjct: 101 QPMPYLVYLQGGPGFECPRPTEASGWIQKACEEFRVILMDQRGTGLSTPLSPSSMSQFQT 160

Query: 125 AEDLANYLKHFRADNIVNDAEFIRTRLVPDAGPWTILGQSFGGFCAVTYLSFAPQGLKQV 184
           AEDLANYLKHFRADNIVNDAEFIRTRLVPDA PWTILGQS+GGFCAVTYLSFAPQGLKQV
Sbjct: 161 AEDLANYLKHFRADNIVNDAEFIRTRLVPDAAPWTILGQSYGGFCAVTYLSFAPQGLKQV 220

Query: 185 LITGGIPPIGNKCTADSVYRACFEKVIIQNEKYYKRYPQDVEIIREVAKYLAEHGGGVVL 244
           LITGGIPPIGN CTADSVYRACFEK+IIQNEKYYKRYPQDV+I+ EV KYL E+GGGV L
Sbjct: 221 LITGGIPPIGNGCTADSVYRACFEKIIIQNEKYYKRYPQDVKIVHEVVKYLEENGGGVPL 280

Query: 245 PSGGILTPKGLQVLGLFALGSSTGFERLHYLFERVWDPVIVPGAPKRISSFFLRACDNWL 304
           P GGILTPKGLQ LGL ALGSSTGFER+HYLFERVWDP+IVPGAPKRIS FFL A   WL
Sbjct: 281 PCGGILTPKGLQTLGLSALGSSTGFERMHYLFERVWDPIIVPGAPKRISYFFLNAISGWL 340

Query: 305 SLDSNPLYVLLHESIYCQGASSRWSAQRIKNELKNKFDANNALKEGCPMFFTGEMVFPWM 364
           SLDSNPLY L+HESIYCQGASSRWSAQRI+NEL+NKFD   A+KEGCP++FTGEM+FPWM
Sbjct: 341 SLDSNPLYGLMHESIYCQGASSRWSAQRIRNELENKFDVIRAVKEGCPVYFTGEMIFPWM 400

Query: 365 FDEIHALRPLKDAARILAEKEDWPPLYDVAALRNNKVPVAAAVYYEDMFVNFKLAMETAS 424
           FDEIHAL+P KDAA ILAEKEDWPPLYD+AAL+NNKVPVAAAVYYEDM+VNFKLAMETAS
Sbjct: 401 FDEIHALKPFKDAANILAEKEDWPPLYDIAALKNNKVPVAAAVYYEDMYVNFKLAMETAS 460

Query: 425 QIAGIRLWITNEYMHSGLRDAGPQVLDHLMGLLNGKKPLF 465
           QIAGIRLW+TNE+MHSGLRD GPQVLDHLMGLLNGKKPLF
Sbjct: 461 QIAGIRLWVTNEFMHSGLRDGGPQVLDHLMGLLNGKKPLF 499

BLAST of Moc05g04160 vs. ExPASy Swiss-Prot
Match: P46547 (Proline iminopeptidase OS=Aeromonas sobria OX=646 GN=pip PE=1 SV=3)

HSP 1 Score: 382.5 bits (981), Expect = 6.8e-105
Identity = 199/435 (45.75%), Postives = 275/435 (63.22%), Query Frame = 0

Query: 22  YSVPELRLRDHHFTVPLDYSLDQDASPKISVFAREVVLVGKEEQSMPYLLYLQGGPGFES 81
           Y +  +    H FTVPLD+    D    I++F R +    + +  +P+LLYLQGGPGF +
Sbjct: 7   YVLDGIHCEPHFFTVPLDHQ-QPDDEETITLFGRTLCRKDRLDDELPWLLYLQGGPGFGA 66

Query: 82  PRPTEASGWMQKACEEFRVVLMDQRGTGLSTPLTSSSMSQFQSAEDLANYLKHFRADNIV 141
           PRP+   GW+++A +EFRV+L+DQRGTG STP+ +  ++     +  A+YL HFRAD+IV
Sbjct: 67  PRPSANGGWIKRALQEFRVLLLDQRGTGHSTPIHAELLAHLNPRQQ-ADYLSHFRADSIV 126

Query: 142 NDAEFIRTRLVPDAGPWTILGQSFGGFCAVTYLSFAPQGLKQVLITGGIPPIGNKCTADS 201
            DAE IR +L PD  PW++LGQSFGGFC++TYLS  P  L +V +TGG+ PIG   +AD 
Sbjct: 127 RDAELIREQLSPD-HPWSLLGQSFGGFCSLTYLSLFPDSLHEVYLTGGVAPIGR--SADE 186

Query: 202 VYRACFEKVIIQNEKYYKRYPQDVEIIREVAKYLAEHGGGVVLPSGGILTPKGLQVLGLF 261
           VYRA +++V  +N  ++ R+P    I   +A +L  H   V LP+G  LT + LQ  GL 
Sbjct: 187 VYRATYQRVADKNRAFFARFPHAQAIANRLATHLQRH--DVRLPNGQRLTVEQLQQQGL- 246

Query: 262 ALGSSTGFERLHYLFERVWDPVIVPGAPKRISSFFLRACDNWLSLDSNPLYVLLHESIYC 321
            LG+S  FE L+YL E  +         ++++  FL         ++NP++ +LHE IYC
Sbjct: 247 DLGASGAFEELYYLLEDAF-------IGEKLNPAFLYQVQAMQPFNTNPVFAILHELIYC 306

Query: 322 QGASSRWSAQRIKNELKNKFDANNALKEGCPMFFTGEMVFPWMFDEIHALRPLKDAARIL 381
           +GA+S W+A+R++ E    F A  A  +G    FTGEM+FPWMF++   L PLK+AA +L
Sbjct: 307 EGAASHWAAERVRGE----FPA-LAWAQGKDFAFTGEMIFPWMFEQFRELIPLKEAAHLL 366

Query: 382 AEKEDWPPLYDVAALRNNKVPVAAAVYYEDMFVNFKLAMETASQIAGIRLWITNEYMHSG 441
           AEK DW PLYD   L  NKVPVA AVY EDM+V F  + ET   ++  R WITNEY H+G
Sbjct: 367 AEKADWGPLYDPVQLARNKVPVACAVYAEDMYVEFDYSRETLKGLSNSRAWITNEYEHNG 421

Query: 442 LRDAGPQVLDHLMGL 457
           LR  G Q+LD L+ L
Sbjct: 427 LRVDGEQILDRLIRL 421

BLAST of Moc05g04160 vs. ExPASy Swiss-Prot
Match: A0A1L9WUM2 (Proline iminopeptidase aneH OS=Aspergillus aculeatus (strain ATCC 16872 / CBS 172.66 / WB 5094) OX=690307 GN=aneH PE=3 SV=1)

HSP 1 Score: 256.9 bits (655), Expect = 4.3e-67
Identity = 152/436 (34.86%), Postives = 238/436 (54.59%), Query Frame = 0

Query: 28  RLRDHHFTVPLDYSLDQDASPKISVFAREV-VLVGKEEQSMPYLLYLQGGPGFESPRPTE 87
           R  +  F VPL++S   + +  + +FAR +  ++G ++  +P++LYLQGGPG     P E
Sbjct: 25  RTSEWRFEVPLNHSKPDEGT--VRLFARSIHCVLGVDDPELPWMLYLQGGPGLGCKTPLE 84

Query: 88  ASGWMQKACEE-FRVVLMDQRGTGLSTPLTSSSMSQFQSAEDLANYLKHFRADNIVNDAE 147
            + W+    E+ +RV+ +D+RGTG S+P+T+ +++Q    +  A+ LK FRADNIV D E
Sbjct: 85  YA-WLPSILEKGYRVLFLDERGTGQSSPITAKTLAQQGDHKKQADLLKRFRADNIVRDCE 144

Query: 148 FIRTRLVPDA----GPWTILGQSFGGFCAVTYLSFAPQGLKQVLITGGIPPIGNKCTADS 207
            +R  L  DA      W+++  SFGGFCA++Y+S  P  L +V I GG  P+ N+     
Sbjct: 145 AVRKHLYQDAPADQSKWSVMAASFGGFCAISYVSMFPNSLVEVFIGGGPCPMVNE--PGQ 204

Query: 208 VYRACFEKVIIQNEKYYKRYPQDVEIIREVAKYLAEHGGGVVLPSGGILTPKGLQVLGLF 267
           V    F     +NE YYK+YP+DV  ++ + KYL E+    V  S G LTP+  Q LG+ 
Sbjct: 205 VIPRLFAVAARRNEVYYKKYPEDVGRVKRIIKYLKEN---KVALSKGTLTPERFQQLGVM 264

Query: 268 ALGSSTGFERLHYLFERVWDPVIVPGAPKRISSFFLRACDNWLSLDSNPLYVLLHESIYC 327
            LG   G + +H + +R  + +      K +++  L   +N   +  N +Y LL E +YC
Sbjct: 265 -LGLHGGIDYIHGVVQRTDNDL---DMFKFLTAPTLDLIEN-SGMAHNVIYSLLQEPMYC 324

Query: 328 QGASSRWSAQRIKNELKNKFDANNALKE-GCPMFFTGEMVFPWMFDEIHALRPLKDAARI 387
           QG +  W A + +     K D   +L E    ++FTGE +F  MF+    L+ LK  A +
Sbjct: 325 QGKAGGWCADKCR-----KADPRFSLNERNAQIWFTGEAIFSDMFESYDELKDLKPVAEL 384

Query: 388 LAEKEDWPPLYDVAALRNNKVPVAAAVYYEDMFVNFKLAMETASQIAGIRLWITNEYMHS 447
           LA   DW  LY+ A L  N+VPV  A   EDM+V++ L   TAS++  ++  + N + H 
Sbjct: 385 LARSSDWGQLYNEAQLARNEVPVYVATAVEDMYVSYDLGCHTASKVKNLQQVVNNTWYHD 442

Query: 448 GLRDAGPQVLDHLMGL 457
            +     +V+  L  L
Sbjct: 445 AVETKASEVMPALFAL 442

BLAST of Moc05g04160 vs. ExPASy TrEMBL
Match: A0A6J1D184 (uncharacterized protein LOC111016418 OS=Momordica charantia OX=3673 GN=LOC111016418 PE=3 SV=1)

HSP 1 Score: 966.1 bits (2496), Expect = 5.3e-278
Identity = 464/464 (100.00%), Postives = 464/464 (100.00%), Query Frame = 0

Query: 1   MATETNPINAASPPDHATGKWYSVPELRLRDHHFTVPLDYSLDQDASPKISVFAREVVLV 60
           MATETNPINAASPPDHATGKWYSVPELRLRDHHFTVPLDYSLDQDASPKISVFAREVVLV
Sbjct: 54  MATETNPINAASPPDHATGKWYSVPELRLRDHHFTVPLDYSLDQDASPKISVFAREVVLV 113

Query: 61  GKEEQSMPYLLYLQGGPGFESPRPTEASGWMQKACEEFRVVLMDQRGTGLSTPLTSSSMS 120
           GKEEQSMPYLLYLQGGPGFESPRPTEASGWMQKACEEFRVVLMDQRGTGLSTPLTSSSMS
Sbjct: 114 GKEEQSMPYLLYLQGGPGFESPRPTEASGWMQKACEEFRVVLMDQRGTGLSTPLTSSSMS 173

Query: 121 QFQSAEDLANYLKHFRADNIVNDAEFIRTRLVPDAGPWTILGQSFGGFCAVTYLSFAPQG 180
           QFQSAEDLANYLKHFRADNIVNDAEFIRTRLVPDAGPWTILGQSFGGFCAVTYLSFAPQG
Sbjct: 174 QFQSAEDLANYLKHFRADNIVNDAEFIRTRLVPDAGPWTILGQSFGGFCAVTYLSFAPQG 233

Query: 181 LKQVLITGGIPPIGNKCTADSVYRACFEKVIIQNEKYYKRYPQDVEIIREVAKYLAEHGG 240
           LKQVLITGGIPPIGNKCTADSVYRACFEKVIIQNEKYYKRYPQDVEIIREVAKYLAEHGG
Sbjct: 234 LKQVLITGGIPPIGNKCTADSVYRACFEKVIIQNEKYYKRYPQDVEIIREVAKYLAEHGG 293

Query: 241 GVVLPSGGILTPKGLQVLGLFALGSSTGFERLHYLFERVWDPVIVPGAPKRISSFFLRAC 300
           GVVLPSGGILTPKGLQVLGLFALGSSTGFERLHYLFERVWDPVIVPGAPKRISSFFLRAC
Sbjct: 294 GVVLPSGGILTPKGLQVLGLFALGSSTGFERLHYLFERVWDPVIVPGAPKRISSFFLRAC 353

Query: 301 DNWLSLDSNPLYVLLHESIYCQGASSRWSAQRIKNELKNKFDANNALKEGCPMFFTGEMV 360
           DNWLSLDSNPLYVLLHESIYCQGASSRWSAQRIKNELKNKFDANNALKEGCPMFFTGEMV
Sbjct: 354 DNWLSLDSNPLYVLLHESIYCQGASSRWSAQRIKNELKNKFDANNALKEGCPMFFTGEMV 413

Query: 361 FPWMFDEIHALRPLKDAARILAEKEDWPPLYDVAALRNNKVPVAAAVYYEDMFVNFKLAM 420
           FPWMFDEIHALRPLKDAARILAEKEDWPPLYDVAALRNNKVPVAAAVYYEDMFVNFKLAM
Sbjct: 414 FPWMFDEIHALRPLKDAARILAEKEDWPPLYDVAALRNNKVPVAAAVYYEDMFVNFKLAM 473

Query: 421 ETASQIAGIRLWITNEYMHSGLRDAGPQVLDHLMGLLNGKKPLF 465
           ETASQIAGIRLWITNEYMHSGLRDAGPQVLDHLMGLLNGKKPLF
Sbjct: 474 ETASQIAGIRLWITNEYMHSGLRDAGPQVLDHLMGLLNGKKPLF 517

BLAST of Moc05g04160 vs. ExPASy TrEMBL
Match: A0A5D3D1Y5 (Proline iminopeptidase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold434G005010 PE=3 SV=1)

HSP 1 Score: 862.4 bits (2227), Expect = 8.3e-247
Identity = 404/455 (88.79%), Postives = 432/455 (94.95%), Query Frame = 0

Query: 10  AASPPDHATGKWYSVPELRLRDHHFTVPLDYSLDQDASPKISVFAREVVLVGKEEQSMPY 69
           A SPP H  G WYSVPELRLRDHHF+VPL+YSLDQ +S +ISVFAREVV VGKE+Q MPY
Sbjct: 55  APSPPVHVAGTWYSVPELRLRDHHFSVPLNYSLDQGSSTRISVFAREVVSVGKEDQPMPY 114

Query: 70  LLYLQGGPGFESPRPTEASGWMQKACEEFRVVLMDQRGTGLSTPLTSSSMSQFQSAEDLA 129
           LLYLQGGPGFE  RP+EASGW+QKACEEFRV+LMDQRGTGLSTPLT SSMSQF+SAEDLA
Sbjct: 115 LLYLQGGPGFECARPSEASGWIQKACEEFRVILMDQRGTGLSTPLTPSSMSQFRSAEDLA 174

Query: 130 NYLKHFRADNIVNDAEFIRTRLVPDAGPWTILGQSFGGFCAVTYLSFAPQGLKQVLITGG 189
           NYLKHFRADNIVNDAEFIRTRLVPDA PWTILGQS+GGFCAVTYLSFAPQGLKQVLITGG
Sbjct: 175 NYLKHFRADNIVNDAEFIRTRLVPDAAPWTILGQSYGGFCAVTYLSFAPQGLKQVLITGG 234

Query: 190 IPPIGNKCTADSVYRACFEKVIIQNEKYYKRYPQDVEIIREVAKYLAEHGGGVVLPSGGI 249
           IPPIGN CTADSVYRACFEKVIIQNEKYYKRYPQD+EI+REV KYLA++GGGV+LPSGGI
Sbjct: 235 IPPIGNGCTADSVYRACFEKVIIQNEKYYKRYPQDIEIVREVVKYLADNGGGVLLPSGGI 294

Query: 250 LTPKGLQVLGLFALGSSTGFERLHYLFERVWDPVIVPGAPKRISSFFLRACDNWLSLDSN 309
           LTPKGLQ LGL ALG+STGFERLHYLFERVWDP++VPGAPKRIS FFL A DNWLSLDSN
Sbjct: 295 LTPKGLQTLGLSALGTSTGFERLHYLFERVWDPILVPGAPKRISFFFLNAIDNWLSLDSN 354

Query: 310 PLYVLLHESIYCQGASSRWSAQRIKNELKNKFDANNALKEGCPMFFTGEMVFPWMFDEIH 369
           PLYVLLHESIYCQGASSRWSAQRIKNE++NKFDAN A+KEGCP++FTGEM+FPWMFDEIH
Sbjct: 355 PLYVLLHESIYCQGASSRWSAQRIKNEVENKFDANKAVKEGCPVYFTGEMIFPWMFDEIH 414

Query: 370 ALRPLKDAARILAEKEDWPPLYDVAALRNNKVPVAAAVYYEDMFVNFKLAMETASQIAGI 429
           ALRP KDAA ILA+KEDWPPLYD+AAL+NNKVPVAAAVYYEDMFVNFKLAMETASQIAGI
Sbjct: 415 ALRPFKDAAHILADKEDWPPLYDIAALKNNKVPVAAAVYYEDMFVNFKLAMETASQIAGI 474

Query: 430 RLWITNEYMHSGLRDAGPQVLDHLMGLLNGKKPLF 465
           RLWITNE+MHSGLRDAGPQVLDHLMGLLNGKKPLF
Sbjct: 475 RLWITNEFMHSGLRDAGPQVLDHLMGLLNGKKPLF 509

BLAST of Moc05g04160 vs. ExPASy TrEMBL
Match: A0A1S3AUX5 (proline iminopeptidase OS=Cucumis melo OX=3656 GN=LOC103483239 PE=3 SV=1)

HSP 1 Score: 862.4 bits (2227), Expect = 8.3e-247
Identity = 404/455 (88.79%), Postives = 432/455 (94.95%), Query Frame = 0

Query: 10  AASPPDHATGKWYSVPELRLRDHHFTVPLDYSLDQDASPKISVFAREVVLVGKEEQSMPY 69
           A SPP H  G WYSVPELRLRDHHF+VPL+YSLDQ +S +ISVFAREVV VGKE+Q MPY
Sbjct: 55  APSPPVHVAGTWYSVPELRLRDHHFSVPLNYSLDQGSSTRISVFAREVVSVGKEDQPMPY 114

Query: 70  LLYLQGGPGFESPRPTEASGWMQKACEEFRVVLMDQRGTGLSTPLTSSSMSQFQSAEDLA 129
           LLYLQGGPGFE  RP+EASGW+QKACEEFRV+LMDQRGTGLSTPLT SSMSQF+SAEDLA
Sbjct: 115 LLYLQGGPGFECARPSEASGWIQKACEEFRVILMDQRGTGLSTPLTPSSMSQFRSAEDLA 174

Query: 130 NYLKHFRADNIVNDAEFIRTRLVPDAGPWTILGQSFGGFCAVTYLSFAPQGLKQVLITGG 189
           NYLKHFRADNIVNDAEFIRTRLVPDA PWTILGQS+GGFCAVTYLSFAPQGLKQVLITGG
Sbjct: 175 NYLKHFRADNIVNDAEFIRTRLVPDAAPWTILGQSYGGFCAVTYLSFAPQGLKQVLITGG 234

Query: 190 IPPIGNKCTADSVYRACFEKVIIQNEKYYKRYPQDVEIIREVAKYLAEHGGGVVLPSGGI 249
           IPPIGN CTADSVYRACFEKVIIQNEKYYKRYPQD+EI+REV KYLA++GGGV+LPSGGI
Sbjct: 235 IPPIGNGCTADSVYRACFEKVIIQNEKYYKRYPQDIEIVREVVKYLADNGGGVLLPSGGI 294

Query: 250 LTPKGLQVLGLFALGSSTGFERLHYLFERVWDPVIVPGAPKRISSFFLRACDNWLSLDSN 309
           LTPKGLQ LGL ALG+STGFERLHYLFERVWDP++VPGAPKRIS FFL A DNWLSLDSN
Sbjct: 295 LTPKGLQTLGLSALGTSTGFERLHYLFERVWDPILVPGAPKRISFFFLNAIDNWLSLDSN 354

Query: 310 PLYVLLHESIYCQGASSRWSAQRIKNELKNKFDANNALKEGCPMFFTGEMVFPWMFDEIH 369
           PLYVLLHESIYCQGASSRWSAQRIKNE++NKFDAN A+KEGCP++FTGEM+FPWMFDEIH
Sbjct: 355 PLYVLLHESIYCQGASSRWSAQRIKNEVENKFDANKAVKEGCPVYFTGEMIFPWMFDEIH 414

Query: 370 ALRPLKDAARILAEKEDWPPLYDVAALRNNKVPVAAAVYYEDMFVNFKLAMETASQIAGI 429
           ALRP KDAA ILA+KEDWPPLYD+AAL+NNKVPVAAAVYYEDMFVNFKLAMETASQIAGI
Sbjct: 415 ALRPFKDAAHILADKEDWPPLYDIAALKNNKVPVAAAVYYEDMFVNFKLAMETASQIAGI 474

Query: 430 RLWITNEYMHSGLRDAGPQVLDHLMGLLNGKKPLF 465
           RLWITNE+MHSGLRDAGPQVLDHLMGLLNGKKPLF
Sbjct: 475 RLWITNEFMHSGLRDAGPQVLDHLMGLLNGKKPLF 509

BLAST of Moc05g04160 vs. ExPASy TrEMBL
Match: A0A5A7U143 (Proline iminopeptidase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold171G001580 PE=3 SV=1)

HSP 1 Score: 861.3 bits (2224), Expect = 1.9e-246
Identity = 403/455 (88.57%), Postives = 432/455 (94.95%), Query Frame = 0

Query: 10  AASPPDHATGKWYSVPELRLRDHHFTVPLDYSLDQDASPKISVFAREVVLVGKEEQSMPY 69
           A SPP H  G WYSVPELRLRDHHF+VPL+YSLDQ +S +ISVFAREVV VGKE+Q MPY
Sbjct: 9   APSPPVHVAGTWYSVPELRLRDHHFSVPLNYSLDQGSSTRISVFAREVVSVGKEDQPMPY 68

Query: 70  LLYLQGGPGFESPRPTEASGWMQKACEEFRVVLMDQRGTGLSTPLTSSSMSQFQSAEDLA 129
           LLYLQGGPGFE  RP+EASGW+QKAC+EFRV+LMDQRGTGLSTPLT SSMSQF+SAEDLA
Sbjct: 69  LLYLQGGPGFECARPSEASGWIQKACDEFRVILMDQRGTGLSTPLTPSSMSQFRSAEDLA 128

Query: 130 NYLKHFRADNIVNDAEFIRTRLVPDAGPWTILGQSFGGFCAVTYLSFAPQGLKQVLITGG 189
           NYLKHFRADNIVNDAEFIRTRLVPDA PWTILGQS+GGFCAVTYLSFAPQGLKQVLITGG
Sbjct: 129 NYLKHFRADNIVNDAEFIRTRLVPDAAPWTILGQSYGGFCAVTYLSFAPQGLKQVLITGG 188

Query: 190 IPPIGNKCTADSVYRACFEKVIIQNEKYYKRYPQDVEIIREVAKYLAEHGGGVVLPSGGI 249
           IPPIGN CTADSVYRACFEKVIIQNEKYYKRYPQD+EI+REV KYLA++GGGV+LPSGGI
Sbjct: 189 IPPIGNGCTADSVYRACFEKVIIQNEKYYKRYPQDIEIVREVVKYLADNGGGVLLPSGGI 248

Query: 250 LTPKGLQVLGLFALGSSTGFERLHYLFERVWDPVIVPGAPKRISSFFLRACDNWLSLDSN 309
           LTPKGLQ LGL ALG+STGFERLHYLFERVWDP++VPGAPKRIS FFL A DNWLSLDSN
Sbjct: 249 LTPKGLQTLGLSALGTSTGFERLHYLFERVWDPILVPGAPKRISFFFLNAIDNWLSLDSN 308

Query: 310 PLYVLLHESIYCQGASSRWSAQRIKNELKNKFDANNALKEGCPMFFTGEMVFPWMFDEIH 369
           PLYVLLHESIYCQGASSRWSAQRIKNE++NKFDAN A+KEGCP++FTGEM+FPWMFDEIH
Sbjct: 309 PLYVLLHESIYCQGASSRWSAQRIKNEVENKFDANKAVKEGCPVYFTGEMIFPWMFDEIH 368

Query: 370 ALRPLKDAARILAEKEDWPPLYDVAALRNNKVPVAAAVYYEDMFVNFKLAMETASQIAGI 429
           ALRP KDAA ILA+KEDWPPLYD+AAL+NNKVPVAAAVYYEDMFVNFKLAMETASQIAGI
Sbjct: 369 ALRPFKDAAHILADKEDWPPLYDIAALKNNKVPVAAAVYYEDMFVNFKLAMETASQIAGI 428

Query: 430 RLWITNEYMHSGLRDAGPQVLDHLMGLLNGKKPLF 465
           RLWITNE+MHSGLRDAGPQVLDHLMGLLNGKKPLF
Sbjct: 429 RLWITNEFMHSGLRDAGPQVLDHLMGLLNGKKPLF 463

BLAST of Moc05g04160 vs. ExPASy TrEMBL
Match: A0A6J1F4P5 (uncharacterized protein LOC111440690 OS=Cucurbita moschata OX=3662 GN=LOC111440690 PE=3 SV=1)

HSP 1 Score: 852.0 bits (2200), Expect = 1.1e-243
Identity = 399/460 (86.74%), Postives = 429/460 (93.26%), Query Frame = 0

Query: 5   TNPINAASPPDHATGKWYSVPELRLRDHHFTVPLDYSLDQDASPKISVFAREVVLVGKEE 64
           TNP N ASPP+HA G WYSVPELRLRDH+F+VPL+YSLD  +SPKISV+AREVV VGKEE
Sbjct: 41  TNPSNGASPPEHAAGTWYSVPELRLRDHYFSVPLNYSLDH-SSPKISVYAREVVSVGKEE 100

Query: 65  QSMPYLLYLQGGPGFESPRPTEASGWMQKACEEFRVVLMDQRGTGLSTPLTSSSMSQFQS 124
           Q MPYLLYLQGGPGFE PRPTEASGW+QKACEEFRV+LMDQRGTGLSTPL+ SSMSQFQS
Sbjct: 101 QPMPYLLYLQGGPGFECPRPTEASGWIQKACEEFRVILMDQRGTGLSTPLSPSSMSQFQS 160

Query: 125 AEDLANYLKHFRADNIVNDAEFIRTRLVPDAGPWTILGQSFGGFCAVTYLSFAPQGLKQV 184
           AEDLA+YLKHFRADNIVNDAEFIRTRLVPDA PWTILGQS+GGFCAVTYLSFAPQGLKQV
Sbjct: 161 AEDLADYLKHFRADNIVNDAEFIRTRLVPDAAPWTILGQSYGGFCAVTYLSFAPQGLKQV 220

Query: 185 LITGGIPPIGNKCTADSVYRACFEKVIIQNEKYYKRYPQDVEIIREVAKYLAEHGGGVVL 244
           LITGGIPPIGN CTADSVYRACFEK+IIQNEKYYKRYPQDV+I+ EV KYL E+GGG+ L
Sbjct: 221 LITGGIPPIGNGCTADSVYRACFEKIIIQNEKYYKRYPQDVKIVHEVVKYLEENGGGIPL 280

Query: 245 PSGGILTPKGLQVLGLFALGSSTGFERLHYLFERVWDPVIVPGAPKRISSFFLRACDNWL 304
           P GGILTPKGLQ LGL ALGSSTGFER+HYLFERVWDP+IVPGAPKRIS FFL A   WL
Sbjct: 281 PCGGILTPKGLQTLGLSALGSSTGFERMHYLFERVWDPIIVPGAPKRISYFFLNAISGWL 340

Query: 305 SLDSNPLYVLLHESIYCQGASSRWSAQRIKNELKNKFDANNALKEGCPMFFTGEMVFPWM 364
           SLDSNPLY L+HESIYCQGASSRWSAQRI NEL+NKFDA  A+KEGCP++FTGEM+FPWM
Sbjct: 341 SLDSNPLYGLMHESIYCQGASSRWSAQRIMNELENKFDATKAVKEGCPVYFTGEMIFPWM 400

Query: 365 FDEIHALRPLKDAARILAEKEDWPPLYDVAALRNNKVPVAAAVYYEDMFVNFKLAMETAS 424
           FDEIHAL+P KDAA ILAEKEDWPPLYD+AAL+NNKVPVAAAVYYEDM+VNFKLAMETAS
Sbjct: 401 FDEIHALKPFKDAANILAEKEDWPPLYDIAALKNNKVPVAAAVYYEDMYVNFKLAMETAS 460

Query: 425 QIAGIRLWITNEYMHSGLRDAGPQVLDHLMGLLNGKKPLF 465
           QIAGIRLW+TNE+MHSGLRD GPQVLDHLMGLLNGKKPLF
Sbjct: 461 QIAGIRLWVTNEFMHSGLRDGGPQVLDHLMGLLNGKKPLF 499

BLAST of Moc05g04160 vs. TAIR 10
Match: AT3G61540.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 736.1 bits (1899), Expect = 1.7e-212
Identity = 344/454 (75.77%), Postives = 394/454 (86.78%), Query Frame = 0

Query: 12  SPPDHATGKWYSVPELRLRDHHFTVPLDYSLDQDASPKISVFAREVVLVGKEEQSMPYLL 71
           S  +H TGKW+SVPELRLRDH F VPLDYS    +SPKI+VFARE+V VGKEEQ+MPYLL
Sbjct: 65  SKSEHVTGKWFSVPELRLRDHRFIVPLDYS---KSSPKITVFAREIVAVGKEEQAMPYLL 124

Query: 72  YLQGGPGFESPRPTEASGWMQKACEEFRVVLMDQRGTGLSTPLTSSSMSQFQSAEDLANY 131
           YLQGGPGFE PRP+EASGW+Q+ACEEFRVVL+DQRGTGLSTPLT SSM QF+SA++LA+Y
Sbjct: 125 YLQGGPGFEGPRPSEASGWIQRACEEFRVVLLDQRGTGLSTPLTCSSMLQFKSAKELADY 184

Query: 132 LKHFRADNIVNDAEFIRTRLVPDAGPWTILGQSFGGFCAVTYLSFAPQGLKQVLITGGIP 191
           L HFRADNIV DAEFIR RLVP A PWTILGQSFGGFCA+TYLSFAP+GLKQVLITGGIP
Sbjct: 185 LVHFRADNIVKDAEFIRVRLVPKADPWTILGQSFGGFCALTYLSFAPEGLKQVLITGGIP 244

Query: 192 PIGNKCTADSVYRACFEKVIIQNEKYYKRYPQDVEIIREVAKYLAE-HGGGVVLPSGGIL 251
           PIG  CTAD VY A FE+V  QNEKYYKR+PQD+EI+RE+  YLAE  GGGV LPSGGIL
Sbjct: 245 PIGKACTADDVYEAGFEQVARQNEKYYKRFPQDIEIVRELVNYLAESEGGGVPLPSGGIL 304

Query: 252 TPKGLQVLGLFALGSSTGFERLHYLFERVWDPVIVPGAPKRISSFFLRACDNWLSLDSNP 311
           TPKGLQ LGL  LGSSTGFERLHY+ ERVWDP++V GAPK IS FFL A ++W S D+NP
Sbjct: 305 TPKGLQTLGLSGLGSSTGFERLHYMLERVWDPILVTGAPKCISQFFLNAFESWHSFDTNP 364

Query: 312 LYVLLHESIYCQGASSRWSAQRIKNELKNKFDANNALKEGCPMFFTGEMVFPWMFDEIHA 371
           LY LLHE+IYC+GASS WSA R++++ + KFDA  A+KE  P+ FTGEM+FPWMFDEIHA
Sbjct: 365 LYALLHEAIYCEGASSGWSAHRLRDKYEYKFDAMKAVKESQPVLFTGEMIFPWMFDEIHA 424

Query: 372 LRPLKDAARILAEKEDWPPLYDVAALRNNKVPVAAAVYYEDMFVNFKLAMETASQIAGIR 431
           L+P K AA +LA+KEDWPPLYDV  L+NNKVPVAAAVYYEDM+VNFKL  ETAS I+GIR
Sbjct: 425 LKPFKAAADLLAKKEDWPPLYDVPRLQNNKVPVAAAVYYEDMYVNFKLVTETASHISGIR 484

Query: 432 LWITNEYMHSGLRDAGPQVLDHLMGLLNGKKPLF 465
           LW+TNE+MHSGLRDAG Q++DHL+G++NGKKPLF
Sbjct: 485 LWVTNEFMHSGLRDAGRQIIDHLLGMINGKKPLF 515

BLAST of Moc05g04160 vs. TAIR 10
Match: AT2G14260.1 (proline iminopeptidase )

HSP 1 Score: 45.1 bits (105), Expect = 1.8e-04
Identity = 35/120 (29.17%), Postives = 53/120 (44.17%), Query Frame = 0

Query: 70  LLYLQGGPGFESPRPTEASGWMQKACEEFRVVLMDQRGTGLSTPLTSSSMSQFQSAEDLA 129
           +++L GGPG      T  S       E +R+VL DQRG G STP                
Sbjct: 99  VVFLHGGPG----GGTAPSNRRFFDPEFYRIVLFDQRGAGKSTPHAC------------- 158

Query: 130 NYLKHFRADNIVNDAEFIRTRL-VPDAGPWTILGQSFGGFCAVTYLSFAPQGLKQVLITG 189
             L+     ++VND E +R  L +P+   W + G S+G   A+ Y    P  +  +++ G
Sbjct: 159 --LEENTTWDLVNDIEKLREHLKIPE---WLVFGGSWGSTLALAYSQSHPDKVTGLVLRG 196

BLAST of Moc05g04160 vs. TAIR 10
Match: AT2G14260.2 (proline iminopeptidase )

HSP 1 Score: 45.1 bits (105), Expect = 1.8e-04
Identity = 35/120 (29.17%), Postives = 53/120 (44.17%), Query Frame = 0

Query: 70  LLYLQGGPGFESPRPTEASGWMQKACEEFRVVLMDQRGTGLSTPLTSSSMSQFQSAEDLA 129
           +++L GGPG      T  S       E +R+VL DQRG G STP                
Sbjct: 48  VVFLHGGPG----GGTAPSNRRFFDPEFYRIVLFDQRGAGKSTPHAC------------- 107

Query: 130 NYLKHFRADNIVNDAEFIRTRL-VPDAGPWTILGQSFGGFCAVTYLSFAPQGLKQVLITG 189
             L+     ++VND E +R  L +P+   W + G S+G   A+ Y    P  +  +++ G
Sbjct: 108 --LEENTTWDLVNDIEKLREHLKIPE---WLVFGGSWGSTLALAYSQSHPDKVTGLVLRG 145

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022147514.11.1e-277100.00uncharacterized protein LOC111016418 [Momordica charantia][more]
XP_008437982.11.7e-24688.79PREDICTED: proline iminopeptidase [Cucumis melo] >TYK17630.1 proline iminopeptid... [more]
KAA0048938.13.8e-24688.57proline iminopeptidase [Cucumis melo var. makuwa][more]
XP_022933365.12.3e-24386.74uncharacterized protein LOC111440690 [Cucurbita moschata][more]
KAG6597118.16.8e-24386.52Proline iminopeptidase, partial [Cucurbita argyrosperma subsp. sororia][more]
Match NameE-valueIdentityDescription
P465476.8e-10545.75Proline iminopeptidase OS=Aeromonas sobria OX=646 GN=pip PE=1 SV=3[more]
A0A1L9WUM24.3e-6734.86Proline iminopeptidase aneH OS=Aspergillus aculeatus (strain ATCC 16872 / CBS 17... [more]
Match NameE-valueIdentityDescription
A0A6J1D1845.3e-278100.00uncharacterized protein LOC111016418 OS=Momordica charantia OX=3673 GN=LOC111016... [more]
A0A5D3D1Y58.3e-24788.79Proline iminopeptidase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold4... [more]
A0A1S3AUX58.3e-24788.79proline iminopeptidase OS=Cucumis melo OX=3656 GN=LOC103483239 PE=3 SV=1[more]
A0A5A7U1431.9e-24688.57Proline iminopeptidase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold1... [more]
A0A6J1F4P51.1e-24386.74uncharacterized protein LOC111440690 OS=Cucurbita moschata OX=3662 GN=LOC1114406... [more]
Match NameE-valueIdentityDescription
AT3G61540.11.7e-21275.77alpha/beta-Hydrolases superfamily protein [more]
AT2G14260.11.8e-0429.17proline iminopeptidase [more]
AT2G14260.21.8e-0429.17proline iminopeptidase [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (OHB3-1) v2
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002410Peptidase S33PRINTSPR00793PROAMNOPTASEcoord: 100..111
score: 55.56
coord: 70..78
score: 67.13
coord: 160..174
score: 44.44
IPR000073Alpha/beta hydrolase fold-1PFAMPF00561Abhydrolase_1coord: 69..189
e-value: 1.2E-12
score: 48.1
IPR029058Alpha/Beta hydrolase foldGENE3D3.40.50.1820alpha/beta hydrolasecoord: 57..454
e-value: 6.9E-17
score: 64.0
IPR029058Alpha/Beta hydrolase foldSUPERFAMILY53474alpha/beta-Hydrolasescoord: 60..454
NoneNo IPR availablePANTHERPTHR432482-SUCCINYL-6-HYDROXY-2,4-CYCLOHEXADIENE-1-CARBOXYLATE SYNTHASEcoord: 11..464
NoneNo IPR availablePANTHERPTHR43248:SF2PROLYL AMINOPEPTIDASE-RELATEDcoord: 11..464

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Moc05g04160.1Moc05g04160.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006508 proteolysis
molecular_function GO:0008233 peptidase activity