Moc05g03320 (gene) Bitter gourd (OHB3-1) v2

Overview


Name	Moc05g03320
Type	gene
Organism	Momordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
Description	glyoxylate/hydroxypyruvate reductase HPR3-like
Location	chr5: 2324662 .. 2325863 (+)
RNA-Seq Expression	Moc05g03320
Synteny	Moc05g03320

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCACCCGAAAGCCAAGACCAGGATCTTCCTCAAGTTTTGGTTCTAGGTCCTCCCACAGTATTCTCGTGTCTGGAATCCCAATTCCCAAACAGATTCCATTTCCTCAAACCATGGCTTTCCAAACTCCCACTGCCCCAATTCCTCACCTCTTATGCCCAATACGTCCAAGCCTTGATCATCCCAGGTGGCAGTCTCGCACTAACCTCTGCAATTCTCGACTGCCTCCCGTCGCTGAAGCTCGTCGCCACCGCCAGCGCCGGAGTCGATCACTTGGACTTGCCGGAATTACGCCGCCGTGGGGTCGCCGTCGCCTATGCAGGAAACTTGTTCTCTGAAGATGTTGCCGATATGGCGGTCGGATTACTAATCGACGTTCTTAGGAAAGTGTCGGCGGGAGACCGGTTCATGAGGCAAGGGCTTTGGTCTACGAAAGTGAATTTTCCTCTCGGCTTAAAGGTGAAGTCAAAATGCATTCTCTACTATTGCAATTTTCTCTTTATTTTTCGTTTCCAATCACCTAACTAACAAAAGATGCGACAGATTTTGATGTTCCTAATTTGTTAAGCAATGGCTAATGAGTTGATATCTAATGGAAAAGGTGTAGTTGAATATGGATGTTTTAGATTGATGAACTTAGTGGTTGGAAAATTTCACAGTTGAGCGGCAAACGAATTGGGATTGTTGGATTGGGCAGAATAGGGTGTGAAGTTGCGAAAAGGCTGGAGGGTTTTGGGTGCAGAATCTCATATAACTCAAGGACCAAAAAGCCATTAGTTCCATACTCTTACTATTCCAATGTACATGAACTGGCAGCAGAGTGTGAAGCCCTGATCATCTGTTGTGGGTTAACTGAAGAAACCCGCCATATGATCAACAGGGAGGTGATGGTTGCTTTAGGAAAAGATGGAGTGATAATCAACATTGGTCGCGGGGCGATCGTCGATGAGAAGGAGATGATTCGATGTCTGTTACAAGGAGAGATTTGGGGTGCTGGATTGGATGTGTTTGAGAATGAACCTGAGATTCCCGAACAGCTTTTTACTCTTGACAATGTTGTGTTGTCTCCGCATGTTGCTGTGACAACCCACGAGACTTTCCTGAGATTGTCTAAATTAATGGTGGACAACTTAGAGGCATTCTTCTCAAACAGACCTCTGGTTTCTCCTCTGTGGATTAGTGGTGCAGCTGAGGAAGGCTGA

mRNA sequence

ATGGCACCCGAAAGCCAAGACCAGGATCTTCCTCAAGTTTTGGTTCTAGGTCCTCCCACAGTATTCTCGTGTCTGGAATCCCAATTCCCAAACAGATTCCATTTCCTCAAACCATGGCTTTCCAAACTCCCACTGCCCCAATTCCTCACCTCTTATGCCCAATACGTCCAAGCCTTGATCATCCCAGGTGGCAGTCTCGCACTAACCTCTGCAATTCTCGACTGCCTCCCGTCGCTGAAGCTCGTCGCCACCGCCAGCGCCGGAGTCGATCACTTGGACTTGCCGGAATTACGCCGCCGTGGGGTCGCCGTCGCCTATGCAGGAAACTTGTTCTCTGAAGATGTTGCCGATATGGCGGTCGGATTACTAATCGACGTTCTTAGGAAAGTGTCGGCGGGAGACCGGTTCATGAGGCAAGGGCTTTGGTCTACGAAAGTGAATTTTCCTCTCGGCTTAAAGTTGAGCGGCAAACGAATTGGGATTGTTGGATTGGGCAGAATAGGGTGTGAAGTTGCGAAAAGGCTGGAGGGTTTTGGGTGCAGAATCTCATATAACTCAAGGACCAAAAAGCCATTAGTTCCATACTCTTACTATTCCAATGTACATGAACTGGCAGCAGAGTGTGAAGCCCTGATCATCTGTTGTGGGTTAACTGAAGAAACCCGCCATATGATCAACAGGGAGGTGATGGTTGCTTTAGGAAAAGATGGAGTGATAATCAACATTGGTCGCGGGGCGATCGTCGATGAGAAGGAGATGATTCGATGTCTGTTACAAGGAGAGATTTGGGGTGCTGGATTGGATGTGTTTGAGAATGAACCTGAGATTCCCGAACAGCTTTTTACTCTTGACAATGTTGTGTTGTCTCCGCATGTTGCTGTGACAACCCACGAGACTTTCCTGAGATTGTCTAAATTAATGGTGGACAACTTAGAGGCATTCTTCTCAAACAGACCTCTGGTTTCTCCTCTGTGGATTAGTGGTGCAGCTGAGGAAGGCTGA

Coding sequence (CDS)

ATGGCACCCGAAAGCCAAGACCAGGATCTTCCTCAAGTTTTGGTTCTAGGTCCTCCCACAGTATTCTCGTGTCTGGAATCCCAATTCCCAAACAGATTCCATTTCCTCAAACCATGGCTTTCCAAACTCCCACTGCCCCAATTCCTCACCTCTTATGCCCAATACGTCCAAGCCTTGATCATCCCAGGTGGCAGTCTCGCACTAACCTCTGCAATTCTCGACTGCCTCCCGTCGCTGAAGCTCGTCGCCACCGCCAGCGCCGGAGTCGATCACTTGGACTTGCCGGAATTACGCCGCCGTGGGGTCGCCGTCGCCTATGCAGGAAACTTGTTCTCTGAAGATGTTGCCGATATGGCGGTCGGATTACTAATCGACGTTCTTAGGAAAGTGTCGGCGGGAGACCGGTTCATGAGGCAAGGGCTTTGGTCTACGAAAGTGAATTTTCCTCTCGGCTTAAAGTTGAGCGGCAAACGAATTGGGATTGTTGGATTGGGCAGAATAGGGTGTGAAGTTGCGAAAAGGCTGGAGGGTTTTGGGTGCAGAATCTCATATAACTCAAGGACCAAAAAGCCATTAGTTCCATACTCTTACTATTCCAATGTACATGAACTGGCAGCAGAGTGTGAAGCCCTGATCATCTGTTGTGGGTTAACTGAAGAAACCCGCCATATGATCAACAGGGAGGTGATGGTTGCTTTAGGAAAAGATGGAGTGATAATCAACATTGGTCGCGGGGCGATCGTCGATGAGAAGGAGATGATTCGATGTCTGTTACAAGGAGAGATTTGGGGTGCTGGATTGGATGTGTTTGAGAATGAACCTGAGATTCCCGAACAGCTTTTTACTCTTGACAATGTTGTGTTGTCTCCGCATGTTGCTGTGACAACCCACGAGACTTTCCTGAGATTGTCTAAATTAATGGTGGACAACTTAGAGGCATTCTTCTCAAACAGACCTCTGGTTTCTCCTCTGTGGATTAGTGGTGCAGCTGAGGAAGGCTGA

Protein sequence

MAPESQDQDLPQVLVLGPPTVFSCLESQFPNRFHFLKPWLSKLPLPQFLTSYAQYVQALIIPGGSLALTSAILDCLPSLKLVATASAGVDHLDLPELRRRGVAVAYAGNLFSEDVADMAVGLLIDVLRKVSAGDRFMRQGLWSTKVNFPLGLKLSGKRIGIVGLGRIGCEVAKRLEGFGCRISYNSRTKKPLVPYSYYSNVHELAAECEALIICCGLTEETRHMINREVMVALGKDGVIINIGRGAIVDEKEMIRCLLQGEIWGAGLDVFENEPEIPEQLFTLDNVVLSPHVAVTTHETFLRLSKLMVDNLEAFFSNRPLVSPLWISGAAEEG

Homology

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BLAST of Moc05g03320 vs. NCBI nr
Match: XP_022147257.1 (glyoxylate/hydroxypyruvate reductase HPR3-like [Momordica charantia])

HSP 1 Score: 668.3 bits (1723), Expect = 3.4e-188
Identity = 333/333 (100.00%), Postives = 333/333 (100.00%), Query Frame = 0


Query: 1   MAPESQDQDLPQVLVLGPPTVFSCLESQFPNRFHFLKPWLSKLPLPQFLTSYAQYVQALI 60
           MAPESQDQDLPQVLVLGPPTVFSCLESQFPNRFHFLKPWLSKLPLPQFLTSYAQYVQALI
Sbjct: 1   MAPESQDQDLPQVLVLGPPTVFSCLESQFPNRFHFLKPWLSKLPLPQFLTSYAQYVQALI 60

Query: 61  IPGGSLALTSAILDCLPSLKLVATASAGVDHLDLPELRRRGVAVAYAGNLFSEDVADMAV 120
           IPGGSLALTSAILDCLPSLKLVATASAGVDHLDLPELRRRGVAVAYAGNLFSEDVADMAV
Sbjct: 61  IPGGSLALTSAILDCLPSLKLVATASAGVDHLDLPELRRRGVAVAYAGNLFSEDVADMAV 120

Query: 121 GLLIDVLRKVSAGDRFMRQGLWSTKVNFPLGLKLSGKRIGIVGLGRIGCEVAKRLEGFGC 180
           GLLIDVLRKVSAGDRFMRQGLWSTKVNFPLGLKLSGKRIGIVGLGRIGCEVAKRLEGFGC
Sbjct: 121 GLLIDVLRKVSAGDRFMRQGLWSTKVNFPLGLKLSGKRIGIVGLGRIGCEVAKRLEGFGC 180

Query: 181 RISYNSRTKKPLVPYSYYSNVHELAAECEALIICCGLTEETRHMINREVMVALGKDGVII 240
           RISYNSRTKKPLVPYSYYSNVHELAAECEALIICCGLTEETRHMINREVMVALGKDGVII
Sbjct: 181 RISYNSRTKKPLVPYSYYSNVHELAAECEALIICCGLTEETRHMINREVMVALGKDGVII 240

Query: 241 NIGRGAIVDEKEMIRCLLQGEIWGAGLDVFENEPEIPEQLFTLDNVVLSPHVAVTTHETF 300
           NIGRGAIVDEKEMIRCLLQGEIWGAGLDVFENEPEIPEQLFTLDNVVLSPHVAVTTHETF
Sbjct: 241 NIGRGAIVDEKEMIRCLLQGEIWGAGLDVFENEPEIPEQLFTLDNVVLSPHVAVTTHETF 300

Query: 301 LRLSKLMVDNLEAFFSNRPLVSPLWISGAAEEG 334
           LRLSKLMVDNLEAFFSNRPLVSPLWISGAAEEG
Sbjct: 301 LRLSKLMVDNLEAFFSNRPLVSPLWISGAAEEG 333

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BLAST of Moc05g03320 vs. NCBI nr
Match: XP_022159358.1 (glyoxylate/hydroxypyruvate reductase HPR3-like [Momordica charantia])

HSP 1 Score: 532.3 bits (1370), Expect = 2.9e-147
Identity = 266/328 (81.10%), Postives = 289/328 (88.11%), Query Frame = 0


Query: 1   MAPESQDQDLPQVLVLGPPTVFSCLESQFPNRFHFLKPWLSKLPLPQFLTSYAQYVQALI 60
           MA +    +LPQVLVLG P V   LESQF +RFHFLKP  S+ PL QFL+SYAQ VQAL+
Sbjct: 1   MATKIHGDELPQVLVLGSPWVLPSLESQFSHRFHFLKPSNSQFPLLQFLSSYAQSVQALL 60

Query: 61  IPGGSLALTSAILDCLPSLKLVATASAGVDHLDLPELRRRGVAVAYAGNLFSEDVADMAV 120
           IPGGS ALTSAILDCLPSLKL  T S GVDHLDLPELRRRG+AVAYAGNL+SEDVADMAV
Sbjct: 61  IPGGSFALTSAILDCLPSLKLAVTTSVGVDHLDLPELRRRGIAVAYAGNLYSEDVADMAV 120

Query: 121 GLLIDVLRKVSAGDRFMRQGLWSTKVNFPLGLKLSGKRIGIVGLGRIGCEVAKRLEGFGC 180
           GLLIDVLRKVSA DRF+RQGLW T   FPLGLKLSGKRIGIVGLG+IGCEVAKRLEGFGC
Sbjct: 121 GLLIDVLRKVSAADRFLRQGLWPTNREFPLGLKLSGKRIGIVGLGKIGCEVAKRLEGFGC 180

Query: 181 RISYNSRTKKPLVPYSYYSNVHELAAECEALIICCGLTEETRHMINREVMVALGKDGVII 240
           ++SYNSRTKKPLVPYSY+S VHELAAECEALI+CCGLTEETRHMI+REVMVALGKDGVII
Sbjct: 181 KMSYNSRTKKPLVPYSYHSTVHELAAECEALIVCCGLTEETRHMIDREVMVALGKDGVII 240

Query: 241 NIGRGAIVDEKEMIRCLLQGEIWGAGLDVFENEPEIPEQLFTLDNVVLSPHVAVTTHETF 300
           N+GRGAI+DEKE+I CLLQGEI GAGLDVFENEPEIPE+LF +DNVVLSPHVAV THE+ 
Sbjct: 241 NVGRGAIIDEKELIECLLQGEIGGAGLDVFENEPEIPEELFGVDNVVLSPHVAVITHESM 300

Query: 301 LRLSKLMVDNLEAFFSNRPLVSPLWISG 329
             L KL+VDNLEAFFSN+PLVSP    G
Sbjct: 301 KGLCKLVVDNLEAFFSNKPLVSPFMDGG 328

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BLAST of Moc05g03320 vs. NCBI nr
Match: KAG6597182.1 (Glyoxylate/hydroxypyruvate reductase HPR3, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 530.0 bits (1364), Expect = 1.5e-146
Identity = 262/320 (81.88%), Postives = 290/320 (90.62%), Query Frame = 0


Query: 4   ESQDQDLPQVLVLGPPTVFSCLESQFPNRFHFLKPWLSKLPLPQFLTSYAQYVQALIIPG 63
           + Q  +LPQVLVL PP+VFSCLESQFPNRFHFLKPWLSKL L QFLTSYAQ V+AL+  G
Sbjct: 33  DEQTNELPQVLVLEPPSVFSCLESQFPNRFHFLKPWLSKLTLTQFLTSYAQSVRALLCRG 92

Query: 64  GSLALTSAILDCLPSLKLVATASAGVDHLDLPELRRRGVAVAYAGNLFSEDVADMAVGLL 123
           G + LTS ILDCLPSLKLV T+S GVDHLDL ELRRRGVA+AYAGNLFS+DVADMAVGLL
Sbjct: 93  GGVKLTSPILDCLPSLKLVVTSSVGVDHLDLLELRRRGVAIAYAGNLFSQDVADMAVGLL 152

Query: 124 IDVLRKVSAGDRFMRQGLWSTKVNFPLGLKLSGKRIGIVGLGRIGCEVAKRLEGFGCRIS 183
           IDVLRK+SAGDRF+RQGLWST+ NFPLGLKLSGKR+GIVGLG+IG EVAKRLEGF CRIS
Sbjct: 153 IDVLRKLSAGDRFVRQGLWSTERNFPLGLKLSGKRVGIVGLGKIGSEVAKRLEGFNCRIS 212

Query: 184 YNSRTKKPLVPYSYYSNVHELAAECEALIICCGLTEETRHMINREVMVALGKDGVIINIG 243
           YNSRTKK  VPYSY+SNV+ELA  CEALIICCGLTEETRHMINREVM+ALGKDGVIIN+G
Sbjct: 213 YNSRTKKSAVPYSYHSNVYELATNCEALIICCGLTEETRHMINREVMLALGKDGVIINVG 272

Query: 244 RGAIVDEKEMIRCLLQGEIWGAGLDVFENEPEIPEQLFTLDNVVLSPHVAVTTHETFLRL 303
           RGAI++EKEMI+CL+QGEI GAGLDVFENEP I EQLF LDNVVLSPH AV+T+E+ + L
Sbjct: 273 RGAIINEKEMIQCLIQGEIGGAGLDVFENEPNISEQLFNLDNVVLSPHAAVSTYESAVGL 332

Query: 304 SKLMVDNLEAFFSNRPLVSP 324
            KL+VDNLEAFFSN+PLVSP
Sbjct: 333 VKLLVDNLEAFFSNQPLVSP 352

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BLAST of Moc05g03320 vs. NCBI nr
Match: XP_022147048.1 (glyoxylate/hydroxypyruvate reductase HPR3-like [Momordica charantia])

HSP 1 Score: 519.6 bits (1337), Expect = 2.0e-143
Identity = 260/324 (80.25%), Postives = 286/324 (88.27%), Query Frame = 0


Query: 1   MAPESQDQDLPQVLVLGPPTVFSCLESQFPNRFHFLKPWLSKLPLPQFLTSYAQYVQALI 60
           MA E    +LPQVLVL PP  FS LESQFPNRFHFLKPW SKLPL QFLTSYAQ   AL+
Sbjct: 1   MAVEIPADELPQVLVLAPPADFSSLESQFPNRFHFLKPWFSKLPLLQFLTSYAQSAPALL 60

Query: 61  I-PGGSLALTSAILDCLPSLKLVATASAGVDHLDLPELRRRGVAVAYAGNLFSEDVADMA 120
           + PG  LA++S ILDCLPSLKLV T SAGVDHLDLPELRR GVA+AYAGNLFSEDVADMA
Sbjct: 61  VSPGDRLAVSSNILDCLPSLKLVVTISAGVDHLDLPELRRCGVAIAYAGNLFSEDVADMA 120

Query: 121 VGLLIDVLRKVSAGDRFMRQGLWSTKVNFPLGLKLSGKRIGIVGLGRIGCEVAKRLEGFG 180
           VGLLIDVLRKVSAGDRF+R+GLW TK N PLGLKLSGKRIGIVGLG+IG EVAKRLEGFG
Sbjct: 121 VGLLIDVLRKVSAGDRFVRKGLWPTKGNPPLGLKLSGKRIGIVGLGKIGSEVAKRLEGFG 180

Query: 181 CRISYNSRTKKPLVPYSYYSNVHELAAECEALIICCGLTEETRHMINREVMVALGKDGVI 240
           C++SYNSRTKKP+V YSYYSNV+ELA  CEA+IICCGLT+ETRHMINREVM+ALGKDGV+
Sbjct: 181 CKLSYNSRTKKPIVSYSYYSNVYELATNCEAIIICCGLTKETRHMINREVMLALGKDGVM 240

Query: 241 INIGRGAIVDEKEMIRCLLQGEIWGAGLDVFENEPEIPEQLFTLDNVVLSPHVAVTTHET 300
           +N+GRGAI+DEKEMI+CL++GEI G GLDVFENEPEIPEQLFTLDNVVLSPHVAVTT E+
Sbjct: 241 VNVGRGAIIDEKEMIQCLIEGEIGGVGLDVFENEPEIPEQLFTLDNVVLSPHVAVTTKES 300

Query: 301 FLRLSKLMVDNLEAFFSNRPLVSP 324
              L KL  DNLEA F+N+PLVSP
Sbjct: 301 MEGLVKLATDNLEALFANKPLVSP 324

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BLAST of Moc05g03320 vs. NCBI nr
Match: XP_023526607.1 (glyoxylate/hydroxypyruvate reductase HPR3-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 516.5 bits (1329), Expect = 1.7e-142
Identity = 256/324 (79.01%), Postives = 286/324 (88.27%), Query Frame = 0


Query: 1   MAPESQDQDLPQVLVLGPPTVFSCLESQFPNRFHFLKPWLSKLPLPQFLTSYAQYVQALI 60
           M PE Q   LPQVLVLGPP VF  LESQFPNRFHFLKPWLS LPL QFL SYAQ   AL+
Sbjct: 1   MGPEDQANQLPQVLVLGPPPVFPYLESQFPNRFHFLKPWLSHLPLTQFLISYAQSATALL 60

Query: 61  I-PGGSLALTSAILDCLPSLKLVATASAGVDHLDLPELRRRGVAVAYAGNLFSEDVADMA 120
           I  GG++ LTSAILDCLPSL+LV TAS GVDHLDLPELRRRG+A+AYAGN+F+EDVADMA
Sbjct: 61  IRGGGTVQLTSAILDCLPSLQLVVTASVGVDHLDLPELRRRGLAIAYAGNVFTEDVADMA 120

Query: 121 VGLLIDVLRKVSAGDRFMRQGLWSTKVNFPLGLKLSGKRIGIVGLGRIGCEVAKRLEGFG 180
           +GLLIDVLRKVSAGDRF+RQGLWSTK +FPLGLKLS KRIGIVGLG+IG EVAKRLEGFG
Sbjct: 121 IGLLIDVLRKVSAGDRFVRQGLWSTKGDFPLGLKLSSKRIGIVGLGKIGSEVAKRLEGFG 180

Query: 181 CRISYNSRTKKPLVPYSYYSNVHELAAECEALIICCGLTEETRHMINREVMVALGKDGVI 240
           C++SY SR KK + PYSYYSNV+ELAA CE LIICC LTEET H+IN+EVMVALGKDGVI
Sbjct: 181 CKVSYYSRIKKSMAPYSYYSNVYELAANCEVLIICCALTEETHHIINKEVMVALGKDGVI 240

Query: 241 INIGRGAIVDEKEMIRCLLQGEIWGAGLDVFENEPEIPEQLFTLDNVVLSPHVAVTTHET 300
           +NIGRGAI++EKEMIRCL++GEI GAGLDVFENEP IPE+LF LDNVVL+PHVAV T+E+
Sbjct: 241 VNIGRGAIINEKEMIRCLIEGEIGGAGLDVFENEPRIPEELFHLDNVVLTPHVAVGTYES 300

Query: 301 FLRLSKLMVDNLEAFFSNRPLVSP 324
            + LSKLMV+NLE FFSN+ LVSP
Sbjct: 301 RMELSKLMVENLETFFSNKSLVSP 324

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BLAST of Moc05g03320 vs. ExPASy Swiss-Prot
Match: Q9LE33 (Glyoxylate/hydroxypyruvate reductase HPR3 OS=Arabidopsis thaliana OX=3702 GN=HPR3 PE=2 SV=1)

HSP 1 Score: 328.6 bits (841), Expect = 8.4e-89
Identity = 163/315 (51.75%), Postives = 220/315 (69.84%), Query Frame = 0


Query: 11  PQVLVLGPPTVFSCLESQFPNRFH-FLKPWLSKLPLPQFLTSYAQYVQALIIPGGSLALT 70
           P V++L  P   + ++      F   +    S   LP F   +A   +A +I  G L +T
Sbjct: 7   PPVVLLHRPPSLTFMDEILTREFRTLITDTSSSESLPSFFPRHASSARAFVI-SGRLPVT 66

Query: 71  SAILDCLPSLKLVATASAGVDHLDLPELRRRGVAVAYAGNLFSEDVADMAVGLLIDVLRK 130
             +L  LPSL+++   S G+DH+DL   +RRG+ +  AGN FS+DVAD AVGLLI VLR+
Sbjct: 67  DELLSHLPSLQILVCTSVGIDHIDLAACKRRGIVITNAGNAFSDDVADCAVGLLISVLRR 126

Query: 131 VSAGDRFMRQGLWSTKVNFPLGLKLSGKRIGIVGLGRIGCEVAKRLEGFGCRISYNSRTK 190
           + A DR++R G W+   +F LG K+SGKR+GIVGLG IG  VAKRLE FGC ISYNSR++
Sbjct: 127 IPAADRYVRSGNWAKFGDFQLGSKVSGKRVGIVGLGSIGSFVAKRLESFGCVISYNSRSQ 186

Query: 191 KPLVPYSYYSNVHELAAECEALIICCGLTEETRHMINREVMVALGKDGVIINIGRGAIVD 250
           K   PY YYS++  LA   + L++CC LT+ET H++NREVM  LGKDGV+IN+GRG ++D
Sbjct: 187 KQSSPYRYYSDILSLAENNDVLVLCCSLTDETHHIVNREVMELLGKDGVVINVGRGKLID 246

Query: 251 EKEMIRCLLQGEIWGAGLDVFENEPEIPEQLFTLDNVVLSPHVAVTTHETFLRLSKLMVD 310
           EKEM++CL+ G I GAGLDVFENEP +P++LF LDNVVLSPH AV T  +   ++++ + 
Sbjct: 247 EKEMVKCLVDGVIGGAGLDVFENEPAVPQELFGLDNVVLSPHFAVATPGSLDNVAQIALA 306

Query: 311 NLEAFFSNRPLVSPL 325
           NL+AFFSNRPL+SP+
Sbjct: 307 NLKAFFSNRPLLSPV 320

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BLAST of Moc05g03320 vs. ExPASy Swiss-Prot
Match: Q65CJ7 (Hydroxyphenylpyruvate reductase OS=Plectranthus scutellarioides OX=4142 GN=HPPR PE=1 SV=2)

HSP 1 Score: 270.4 bits (690), Expect = 2.7e-71
Identity = 141/312 (45.19%), Postives = 209/312 (66.99%), Query Frame = 0


Query: 13  VLVLGPPTVFSCLESQFPNRFHFLKPWLSKLPLPQFLTSYAQYVQALIIPGGSLALTSAI 72
           VL++ P + +  LE +   RF   + W ++     FL   A+ ++A ++   +    + +
Sbjct: 6   VLMMCPMSTY--LEQELDKRFKLFRYW-TQPAQRDFLALQAESIRA-VVGNSNAGADAEL 65

Query: 73  LDCLPSLKLVATASAGVDHLDLPELRRRGVAVAYAGNLFSEDVADMAVGLLIDVLRKVSA 132
           +D LP L++V++ S G+D +DL +   +GV V    ++ ++DVAD+A+GL++ VLR++  
Sbjct: 66  IDALPKLEIVSSFSVGLDKVDLIKCEEKGVRVTNTPDVLTDDVADLAIGLILAVLRRICE 125

Query: 133 GDRFMRQGLWSTKVNFPLGLKLSGKRIGIVGLGRIGCEVAKRLEGFGCRISYNSRTKKPL 192
            D+++R+G W    +F L  K SGKR+GI+GLGRIG  VA+R E F C ISY SR+KKP 
Sbjct: 126 CDKYVRRGAWKFG-DFKLTTKFSGKRVGIIGLGRIGLAVAERAEAFDCPISYFSRSKKPN 185

Query: 193 VPYSYYSNVHELAAECEALIICCGLTEETRHMINREVMVALGKDGVIINIGRGAIVDEKE 252
             Y+YY +V ELA+  + L++ C LT ET H+INREV+ ALG  GV+INIGRG  VDE E
Sbjct: 186 TNYTYYGSVVELASNSDILVVACPLTPETTHIINREVIDALGPKGVLINIGRGPHVDEPE 245

Query: 253 MIRCLLQGEIWGAGLDVFENEPEIPEQLFTLDNVVLSPHVAVTTHETFLRLSKLMVDNLE 312
           ++  L++G + GAGLDVFE EPE+PE+LF L+NVVL PHV   T ET   ++ L+V NLE
Sbjct: 246 LVSALVEGRLGGAGLDVFEREPEVPEKLFGLENVVLLPHVGSGTVETRKVMADLVVGNLE 305

Query: 313 AFFSNRPLVSPL 325
           A FS +PL++P+
Sbjct: 306 AHFSGKPLLTPV 312

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BLAST of Moc05g03320 vs. ExPASy Swiss-Prot
Match: A5CAL1 (Glyoxylate/hydroxypyruvate/pyruvate reductase 2KGR OS=Vitis vinifera OX=29760 GN=2KGR PE=1 SV=1)

HSP 1 Score: 268.9 bits (686), Expect = 7.9e-71
Identity = 132/302 (43.71%), Postives = 198/302 (65.56%), Query Frame = 0


Query: 25  LESQFPNRFHFLKPWLSKLPLPQFLTSYAQYVQAL--IIPGGSLALTSAILDCLPSLKLV 84
           LE +   RF   + W      P     + ++  ++  ++    +   + +++ LP +++V
Sbjct: 16  LEQELDKRFKLFRFW----DFPSANDLFREHSNSIRAVVGNSFIGADAQMIEALPKMEIV 75

Query: 85  ATASAGVDHLDLPELRRRGVAVAYAGNLFSEDVADMAVGLLIDVLRKVSAGDRFMRQGLW 144
           ++ S G+D +DL   + +G+ V    ++ +EDVAD+A+ L++  LR++   DR++R G W
Sbjct: 76  SSFSVGLDKIDLVRCKEKGIRVTNTPDVLTEDVADLALALILATLRRICESDRYVRSGSW 135

Query: 145 STKVNFPLGLKLSGKRIGIVGLGRIGCEVAKRLEGFGCRISYNSRTKKPLVPYSYYSNVH 204
             K +F L  K +GK +GI+GLGRIG  +AKR EGF C ISY+SRT+KP   Y YY +V 
Sbjct: 136 K-KGDFKLTTKFTGKSVGIIGLGRIGSAIAKRAEGFSCPISYHSRTEKPGTNYKYYPSVV 195

Query: 205 ELAAECEALIICCGLTEETRHMINREVMVALGKDGVIINIGRGAIVDEKEMIRCLLQGEI 264
           ELA+ C+ L++ C LT ETRH+INREV+ ALG  GV+INIGRG  VDE E++  L++G +
Sbjct: 196 ELASNCQILVVACALTPETRHIINREVINALGPKGVVINIGRGLHVDEPELVSALVEGRL 255

Query: 265 WGAGLDVFENEPEIPEQLFTLDNVVLSPHVAVTTHETFLRLSKLMVDNLEAFFSNRPLVS 324
            GAGLDVFENEP +PE+L  +DNVVL PHV   T ET   ++ L++ NLEA F N+PL++
Sbjct: 256 GGAGLDVFENEPNVPEELLAMDNVVLLPHVGSGTVETRKDMADLVLGNLEAHFLNKPLLT 312

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BLAST of Moc05g03320 vs. ExPASy Swiss-Prot
Match: Q9CA90 (Glyoxylate/hydroxypyruvate reductase A HPR2 OS=Arabidopsis thaliana OX=3702 GN=HPR2 PE=1 SV=1)

HSP 1 Score: 260.8 bits (665), Expect = 2.2e-68
Identity = 137/312 (43.91%), Postives = 203/312 (65.06%), Query Frame = 0


Query: 13  VLVLGPPTVFSCLESQFPNRFHFLKPWLSKLPLPQFLTSYAQYVQALIIPGGSLALTSAI 72
           VL++ P  + S LE++   RF+ L+ W S  P    L    +     ++   S    + +
Sbjct: 6   VLMMCP--MSSYLENELEKRFNLLRFWTS--PEKSVLLETHRNSIRAVVGNASAGADAQL 65

Query: 73  LDCLPSLKLVATASAGVDHLDLPELRRRGVAVAYAGNLFSEDVADMAVGLLIDVLRKVSA 132
           +  LP+L++V++ S G+D +DL + + +G+ V    ++ +EDVAD+A+GL++ +LR++  
Sbjct: 66  ISDLPNLEIVSSFSVGLDKIDLGKCKEKGIRVTNTPDVLTEDVADLAIGLILALLRRLCE 125

Query: 133 GDRFMRQGLWSTKVNFPLGLKLSGKRIGIVGLGRIGCEVAKRLEGFGCRISYNSRTKKPL 192
            DR++R G W  +  F L  K SGK +GI+GLGRIG  +AKR E F C I+Y SRT KP 
Sbjct: 126 CDRYVRSGKWK-QGEFQLTTKFSGKSVGIIGLGRIGTAIAKRAEAFSCPINYYSRTIKPD 185

Query: 193 VPYSYYSNVHELAAECEALIICCGLTEETRHMINREVMVALGKDGVIINIGRGAIVDEKE 252
           V Y YY  V +LA   + L++ C LTE+TRH+++R+VM ALG  GV+INIGRG  VDE+E
Sbjct: 186 VAYKYYPTVVDLAQNSDILVVACPLTEQTRHIVDRQVMDALGAKGVLINIGRGPHVDEQE 245

Query: 253 MIRCLLQGEIWGAGLDVFENEPEIPEQLFTLDNVVLSPHVAVTTHETFLRLSKLMVDNLE 312
           +I+ L +G + GA LDVFE EP +PE+LF L+NVVL PHV   T ET   ++ L+V NLE
Sbjct: 246 LIKALTEGRLGGAALDVFEQEPHVPEELFGLENVVLLPHVGSGTVETRNAMADLVVGNLE 305

Query: 313 AFFSNRPLVSPL 325
           A FS + L++P+
Sbjct: 306 AHFSGKSLLTPV 312

[Close]

BLAST of Moc05g03320 vs. ExPASy Swiss-Prot
Match: Q5FTU6 (2-ketogluconate reductase OS=Gluconobacter oxydans (strain 621H) OX=290633 GN=GOX0417 PE=1 SV=1)

HSP 1 Score: 206.1 bits (523), Expect = 6.3e-52
Identity = 116/316 (36.71%), Postives = 180/316 (56.96%), Query Frame = 0


Query: 11  PQVLVLGP--PTVFSCLESQFPNRFHFLKPWLSKLPLPQFLTSYAQYVQALIIPGGSLAL 70
           P +L + P  P +   LE  F      L P+ S     + L + A  ++ +   GGS  +
Sbjct: 5   PDILTIDPLVPVMKERLEKSFT-----LHPYTSL----ENLKNIAPAIRGITTGGGS-GV 64

Query: 71  TSAILDCLPSLKLVATASAGVDHLDLPELRRRGVAVAYAGNLFSEDVADMAVGLLIDVLR 130
            S I+D LP+L++++    G D ++L E RRR + VA   N  ++DVADMAV L++ V+R
Sbjct: 65  PSEIMDALPNLEVISVNGVGTDRINLDEARRRNIGVAITQNTLTDDVADMAVALMMAVMR 124

Query: 131 KVSAGDRFMRQGLWSTKVNFPLGLKLSGKRIGIVGLGRIGCEVAKRLEGFGCRISYNSRT 190
            +   D F+R G W +    PLG  L+ K++GI G G IG  +AKR+  FG  ++Y +  
Sbjct: 125 SIVTNDAFVRAGKWPS-ATAPLGRSLTRKKVGIAGFGHIGQAIAKRVSAFGMEVAYFNSH 184

Query: 191 KKPLVPYSYYSNVHELAAECEALIICCGLTEETRHMINREVMVALGKDGVIINIGRGAIV 250
            +P     +  ++  LA  C+ LI+       + +MI+R+ + ALGKDG ++NI RG +V
Sbjct: 185 ARPESTCHFEPDLKALATWCDVLILAVSGGPRSANMIDRDTLDALGKDGFLVNIARGTVV 244

Query: 251 DEKEMIRCLLQGEIWGAGLDVFENEPEIPEQLFTLDNVVLSPHVAVTTHETFLRLSKLMV 310
           DE  ++  L +  I GAGLDVF+NEP I     +L N VL  H A  T ET   ++ L+V
Sbjct: 245 DEAALLSALQEKRIAGAGLDVFQNEPNINPAFLSLPNTVLQAHQASATVETRTTMANLVV 304

Query: 311 DNLEAFFSNRPLVSPL 325
           DNL A+F+++ L++P+
Sbjct: 305 DNLIAYFTDKTLLTPV 309

[Close]

BLAST of Moc05g03320 vs. ExPASy TrEMBL
Match: A0A6J1D1V9 (glyoxylate/hydroxypyruvate reductase HPR3-like OS=Momordica charantia OX=3673 GN=LOC111016253 PE=3 SV=1)

HSP 1 Score: 668.3 bits (1723), Expect = 1.7e-188
Identity = 333/333 (100.00%), Postives = 333/333 (100.00%), Query Frame = 0


Query: 1   MAPESQDQDLPQVLVLGPPTVFSCLESQFPNRFHFLKPWLSKLPLPQFLTSYAQYVQALI 60
           MAPESQDQDLPQVLVLGPPTVFSCLESQFPNRFHFLKPWLSKLPLPQFLTSYAQYVQALI
Sbjct: 1   MAPESQDQDLPQVLVLGPPTVFSCLESQFPNRFHFLKPWLSKLPLPQFLTSYAQYVQALI 60

Query: 61  IPGGSLALTSAILDCLPSLKLVATASAGVDHLDLPELRRRGVAVAYAGNLFSEDVADMAV 120
           IPGGSLALTSAILDCLPSLKLVATASAGVDHLDLPELRRRGVAVAYAGNLFSEDVADMAV
Sbjct: 61  IPGGSLALTSAILDCLPSLKLVATASAGVDHLDLPELRRRGVAVAYAGNLFSEDVADMAV 120

Query: 121 GLLIDVLRKVSAGDRFMRQGLWSTKVNFPLGLKLSGKRIGIVGLGRIGCEVAKRLEGFGC 180
           GLLIDVLRKVSAGDRFMRQGLWSTKVNFPLGLKLSGKRIGIVGLGRIGCEVAKRLEGFGC
Sbjct: 121 GLLIDVLRKVSAGDRFMRQGLWSTKVNFPLGLKLSGKRIGIVGLGRIGCEVAKRLEGFGC 180

Query: 181 RISYNSRTKKPLVPYSYYSNVHELAAECEALIICCGLTEETRHMINREVMVALGKDGVII 240
           RISYNSRTKKPLVPYSYYSNVHELAAECEALIICCGLTEETRHMINREVMVALGKDGVII
Sbjct: 181 RISYNSRTKKPLVPYSYYSNVHELAAECEALIICCGLTEETRHMINREVMVALGKDGVII 240

Query: 241 NIGRGAIVDEKEMIRCLLQGEIWGAGLDVFENEPEIPEQLFTLDNVVLSPHVAVTTHETF 300
           NIGRGAIVDEKEMIRCLLQGEIWGAGLDVFENEPEIPEQLFTLDNVVLSPHVAVTTHETF
Sbjct: 241 NIGRGAIVDEKEMIRCLLQGEIWGAGLDVFENEPEIPEQLFTLDNVVLSPHVAVTTHETF 300

Query: 301 LRLSKLMVDNLEAFFSNRPLVSPLWISGAAEEG 334
           LRLSKLMVDNLEAFFSNRPLVSPLWISGAAEEG
Sbjct: 301 LRLSKLMVDNLEAFFSNRPLVSPLWISGAAEEG 333

[Close]

BLAST of Moc05g03320 vs. ExPASy TrEMBL
Match: A0A6J1DYK8 (glyoxylate/hydroxypyruvate reductase HPR3-like OS=Momordica charantia OX=3673 GN=LOC111025775 PE=3 SV=1)

HSP 1 Score: 532.3 bits (1370), Expect = 1.4e-147
Identity = 266/328 (81.10%), Postives = 289/328 (88.11%), Query Frame = 0


Query: 1   MAPESQDQDLPQVLVLGPPTVFSCLESQFPNRFHFLKPWLSKLPLPQFLTSYAQYVQALI 60
           MA +    +LPQVLVLG P V   LESQF +RFHFLKP  S+ PL QFL+SYAQ VQAL+
Sbjct: 1   MATKIHGDELPQVLVLGSPWVLPSLESQFSHRFHFLKPSNSQFPLLQFLSSYAQSVQALL 60

Query: 61  IPGGSLALTSAILDCLPSLKLVATASAGVDHLDLPELRRRGVAVAYAGNLFSEDVADMAV 120
           IPGGS ALTSAILDCLPSLKL  T S GVDHLDLPELRRRG+AVAYAGNL+SEDVADMAV
Sbjct: 61  IPGGSFALTSAILDCLPSLKLAVTTSVGVDHLDLPELRRRGIAVAYAGNLYSEDVADMAV 120

Query: 121 GLLIDVLRKVSAGDRFMRQGLWSTKVNFPLGLKLSGKRIGIVGLGRIGCEVAKRLEGFGC 180
           GLLIDVLRKVSA DRF+RQGLW T   FPLGLKLSGKRIGIVGLG+IGCEVAKRLEGFGC
Sbjct: 121 GLLIDVLRKVSAADRFLRQGLWPTNREFPLGLKLSGKRIGIVGLGKIGCEVAKRLEGFGC 180

Query: 181 RISYNSRTKKPLVPYSYYSNVHELAAECEALIICCGLTEETRHMINREVMVALGKDGVII 240
           ++SYNSRTKKPLVPYSY+S VHELAAECEALI+CCGLTEETRHMI+REVMVALGKDGVII
Sbjct: 181 KMSYNSRTKKPLVPYSYHSTVHELAAECEALIVCCGLTEETRHMIDREVMVALGKDGVII 240

Query: 241 NIGRGAIVDEKEMIRCLLQGEIWGAGLDVFENEPEIPEQLFTLDNVVLSPHVAVTTHETF 300
           N+GRGAI+DEKE+I CLLQGEI GAGLDVFENEPEIPE+LF +DNVVLSPHVAV THE+ 
Sbjct: 241 NVGRGAIIDEKELIECLLQGEIGGAGLDVFENEPEIPEELFGVDNVVLSPHVAVITHESM 300

Query: 301 LRLSKLMVDNLEAFFSNRPLVSPLWISG 329
             L KL+VDNLEAFFSN+PLVSP    G
Sbjct: 301 KGLCKLVVDNLEAFFSNKPLVSPFMDGG 328

[Close]

BLAST of Moc05g03320 vs. ExPASy TrEMBL
Match: A0A6J1D073 (glyoxylate/hydroxypyruvate reductase HPR3-like OS=Momordica charantia OX=3673 GN=LOC111016079 PE=3 SV=1)

HSP 1 Score: 519.6 bits (1337), Expect = 9.5e-144
Identity = 260/324 (80.25%), Postives = 286/324 (88.27%), Query Frame = 0


Query: 1   MAPESQDQDLPQVLVLGPPTVFSCLESQFPNRFHFLKPWLSKLPLPQFLTSYAQYVQALI 60
           MA E    +LPQVLVL PP  FS LESQFPNRFHFLKPW SKLPL QFLTSYAQ   AL+
Sbjct: 1   MAVEIPADELPQVLVLAPPADFSSLESQFPNRFHFLKPWFSKLPLLQFLTSYAQSAPALL 60

Query: 61  I-PGGSLALTSAILDCLPSLKLVATASAGVDHLDLPELRRRGVAVAYAGNLFSEDVADMA 120
           + PG  LA++S ILDCLPSLKLV T SAGVDHLDLPELRR GVA+AYAGNLFSEDVADMA
Sbjct: 61  VSPGDRLAVSSNILDCLPSLKLVVTISAGVDHLDLPELRRCGVAIAYAGNLFSEDVADMA 120

Query: 121 VGLLIDVLRKVSAGDRFMRQGLWSTKVNFPLGLKLSGKRIGIVGLGRIGCEVAKRLEGFG 180
           VGLLIDVLRKVSAGDRF+R+GLW TK N PLGLKLSGKRIGIVGLG+IG EVAKRLEGFG
Sbjct: 121 VGLLIDVLRKVSAGDRFVRKGLWPTKGNPPLGLKLSGKRIGIVGLGKIGSEVAKRLEGFG 180

Query: 181 CRISYNSRTKKPLVPYSYYSNVHELAAECEALIICCGLTEETRHMINREVMVALGKDGVI 240
           C++SYNSRTKKP+V YSYYSNV+ELA  CEA+IICCGLT+ETRHMINREVM+ALGKDGV+
Sbjct: 181 CKLSYNSRTKKPIVSYSYYSNVYELATNCEAIIICCGLTKETRHMINREVMLALGKDGVM 240

Query: 241 INIGRGAIVDEKEMIRCLLQGEIWGAGLDVFENEPEIPEQLFTLDNVVLSPHVAVTTHET 300
           +N+GRGAI+DEKEMI+CL++GEI G GLDVFENEPEIPEQLFTLDNVVLSPHVAVTT E+
Sbjct: 241 VNVGRGAIIDEKEMIQCLIEGEIGGVGLDVFENEPEIPEQLFTLDNVVLSPHVAVTTKES 300

Query: 301 FLRLSKLMVDNLEAFFSNRPLVSP 324
              L KL  DNLEA F+N+PLVSP
Sbjct: 301 MEGLVKLATDNLEALFANKPLVSP 324

[Close]

BLAST of Moc05g03320 vs. ExPASy TrEMBL
Match: A0A6J1EEZ0 (glyoxylate/hydroxypyruvate reductase HPR3-like OS=Cucurbita moschata OX=3662 GN=LOC111431925 PE=3 SV=1)

HSP 1 Score: 513.8 bits (1322), Expect = 5.2e-142
Identity = 257/324 (79.32%), Postives = 286/324 (88.27%), Query Frame = 0


Query: 1   MAPESQDQDLPQVLVLGPPTVFSCLESQFPNRFHFLKPWLSKLPLPQFLTSYAQYVQALI 60
           M PE Q  +LPQVLVLGPP VF  LESQFPNRFHFLKPWLS LPL QFL SYAQ   AL+
Sbjct: 1   MGPEDQANELPQVLVLGPPPVFPYLESQFPNRFHFLKPWLSNLPLTQFLISYAQSATALL 60

Query: 61  I-PGGSLALTSAILDCLPSLKLVATASAGVDHLDLPELRRRGVAVAYAGNLFSEDVADMA 120
           I  GG++ LTSAILDCLPSL+LV TAS GVDHLDLPELRRRG+A+AYAGN+FSEDVADMA
Sbjct: 61  IRGGGTVQLTSAILDCLPSLQLVVTASVGVDHLDLPELRRRGLAIAYAGNVFSEDVADMA 120

Query: 121 VGLLIDVLRKVSAGDRFMRQGLWSTKVNFPLGLKLSGKRIGIVGLGRIGCEVAKRLEGFG 180
           +GLLIDV+RKVSAGDRF+RQGL STK +FPLGLKLS KRIGIVGLG+IG EVAKRLEGFG
Sbjct: 121 IGLLIDVIRKVSAGDRFVRQGLRSTKGDFPLGLKLSSKRIGIVGLGQIGSEVAKRLEGFG 180

Query: 181 CRISYNSRTKKPLVPYSYYSNVHELAAECEALIICCGLTEETRHMINREVMVALGKDGVI 240
           C+ISY SR KK + PYSYYSNV+ELAA CE LIICC LTEET H+INREVMVALGKDGVI
Sbjct: 181 CKISYYSRIKKSMAPYSYYSNVYELAANCEVLIICCALTEETHHIINREVMVALGKDGVI 240

Query: 241 INIGRGAIVDEKEMIRCLLQGEIWGAGLDVFENEPEIPEQLFTLDNVVLSPHVAVTTHET 300
           +NIGRGAI++EKEMIRCL++GEI GAGLDVFENEP IPE+LF LDNVVL+PHVAV T+E+
Sbjct: 241 VNIGRGAIINEKEMIRCLIEGEIGGAGLDVFENEPRIPEELFHLDNVVLTPHVAVGTYES 300

Query: 301 FLRLSKLMVDNLEAFFSNRPLVSP 324
            + LSKLMV+NLE FFSN+ LVSP
Sbjct: 301 RMELSKLMVENLETFFSNKSLVSP 324

[Close]

BLAST of Moc05g03320 vs. ExPASy TrEMBL
Match: A0A0A0L908 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G124900 PE=3 SV=1)

HSP 1 Score: 508.4 bits (1308), Expect = 2.2e-140
Identity = 255/323 (78.95%), Postives = 285/323 (88.24%), Query Frame = 0


Query: 1   MAPESQDQDLPQVLVLGPPTVFSCLESQFPNRFHFLKPWLSKLPLPQFLTSYAQYVQALI 60
           MA E + ++LP+VLVLGPP VFS LESQFPN+FH+LKPWLS+LPL QFLTSYAQ  QAL+
Sbjct: 1   MAMEGESKELPEVLVLGPPWVFSTLESQFPNKFHYLKPWLSQLPLHQFLTSYAQSTQALL 60

Query: 61  IPGGSLALTSAILDCLPSLKLVATASAGVDHLDLPELRRRGVAVAYAGNLFSEDVADMAV 120
           IP  S  L S ILDCLPSLKLV T SAGV+HL+  ELR RG+AVAYAGN+FSEDVADMAV
Sbjct: 61  IP-VSPPLNSPILDCLPSLKLVVTVSAGVEHLNFAELRGRGIAVAYAGNVFSEDVADMAV 120

Query: 121 GLLIDVLRKVSAGDRFMRQGLWSTKVNFPLGLKLSGKRIGIVGLGRIGCEVAKRLEGFGC 180
           GLLIDVLRKVSAGDRF++Q L   K +FPL  KLSGK+IGIVGLG+IG EVAKRLEGFGC
Sbjct: 121 GLLIDVLRKVSAGDRFVKQRLQPIKPDFPLRSKLSGKQIGIVGLGKIGSEVAKRLEGFGC 180

Query: 181 RISYNSRTKKPLVPYSYYSNVHELAAECEALIICCGLTEETRHMINREVMVALGKDGVII 240
           RISYNSRTKKPLV YSYYSNVHELA  CE LIICCGLTEET HMINREVM+ LGKDGVII
Sbjct: 181 RISYNSRTKKPLVSYSYYSNVHELATNCEVLIICCGLTEETHHMINREVMLELGKDGVII 240

Query: 241 NIGRGAIVDEKEMIRCLLQGEIWGAGLDVFENEPEIPEQLFTLDNVVLSPHVAVTTHETF 300
           NIGRGA++DEKEMIRCL++GEI GAGLDVFE EPEIP+QLFTLDNVVLSPH AVTTHE+F
Sbjct: 241 NIGRGAVIDEKEMIRCLIEGEIGGAGLDVFEFEPEIPKQLFTLDNVVLSPHTAVTTHESF 300

Query: 301 LRLSKLMVDNLEAFFSNRPLVSP 324
           + ++KL V+NLEAFFSN+PL+SP
Sbjct: 301 VGIAKLAVENLEAFFSNKPLLSP 322

[Close]

BLAST of Moc05g03320 vs. TAIR 10
Match: AT2G45630.2 (D-isomer specific 2-hydroxyacid dehydrogenase family protein )

HSP 1 Score: 353.6 bits (906), Expect = 1.7e-97
Identity = 179/327 (54.74%), Postives = 240/327 (73.39%), Query Frame = 0


Query: 1   MAPESQDQDLPQVLVLGPPTVFSCLESQF--PNRFHFLKPWLSKLPLPQFLTSYAQYVQA 60
           MA  S  + LP+VL++  P   + L   F    +F  LK + S LPLP+FL  ++  + A
Sbjct: 12  MATSSSTEKLPRVLIVKRPDAMAVLGDGFVASTKFEILKAFESPLPLPEFLAYHSDSISA 71

Query: 61  LIIPGGSLALTSAILDCLPSLKLVATASAGVDHLDLPELRRRGVAVAYAGNLFSEDVADM 120
           +I P  +  +T+ ++  LP+L+LV T SAGVDH+DL E RRRG++VA AG+ FSEDVAD 
Sbjct: 72  IIAPVAA-PVTADLIRILPNLRLVVTTSAGVDHVDLVECRRRGISVANAGSSFSEDVADT 131

Query: 121 AVGLLIDVLRKVSAGDRFMRQGLWSTKVNFPLGLKLSGKRIGIVGLGRIGCEVAKRLEGF 180
           AVGLLIDV R++SA +RF++Q  W  K ++PLG KL  KRIGIVGLG IG +VA RL+ F
Sbjct: 132 AVGLLIDVFRRISAANRFVKQRFWPLKGDYPLGSKLGRKRIGIVGLGSIGSKVATRLDAF 191

Query: 181 GCRISYNSRTKKPL-VPYSYYSNVHELAAECEALIICCGLTEETRHMINREVMVALGKDG 240
           GC+ISY+SR +KP  VPY YY ++ E+AA  +ALIICC L E+T  +IN++V+ ALGK G
Sbjct: 192 GCQISYSSRNRKPYDVPYHYYMDIEEMAANSDALIICCELNEKTLRLINKDVLSALGKRG 251

Query: 241 VIINIGRGAIVDEKEMIRCLLQGEIWGAGLDVFENEPEIPEQLFTLDNVVLSPHVAVTTH 300
           VI+N+ RGAI+DE+EM+RCL +GEI GAGLDVFE+EP +P++LF LDNVV SPH A  T 
Sbjct: 252 VIVNVARGAIIDEEEMVRCLREGEIGGAGLDVFEDEPNVPKELFELDNVVFSPHSAFMTL 311

Query: 301 ETFLRLSKLMVDNLEAFFSNRPLVSPL 325
           E    L K++V N+EAFFSN+PL++P+
Sbjct: 312 EGLEELGKVVVGNIEAFFSNKPLLTPV 337

[Close]

BLAST of Moc05g03320 vs. TAIR 10
Match: AT1G12550.1 (D-isomer specific 2-hydroxyacid dehydrogenase family protein )

HSP 1 Score: 328.6 bits (841), Expect = 6.0e-90
Identity = 163/315 (51.75%), Postives = 220/315 (69.84%), Query Frame = 0


Query: 11  PQVLVLGPPTVFSCLESQFPNRFH-FLKPWLSKLPLPQFLTSYAQYVQALIIPGGSLALT 70
           P V++L  P   + ++      F   +    S   LP F   +A   +A +I  G L +T
Sbjct: 7   PPVVLLHRPPSLTFMDEILTREFRTLITDTSSSESLPSFFPRHASSARAFVI-SGRLPVT 66

Query: 71  SAILDCLPSLKLVATASAGVDHLDLPELRRRGVAVAYAGNLFSEDVADMAVGLLIDVLRK 130
             +L  LPSL+++   S G+DH+DL   +RRG+ +  AGN FS+DVAD AVGLLI VLR+
Sbjct: 67  DELLSHLPSLQILVCTSVGIDHIDLAACKRRGIVITNAGNAFSDDVADCAVGLLISVLRR 126

Query: 131 VSAGDRFMRQGLWSTKVNFPLGLKLSGKRIGIVGLGRIGCEVAKRLEGFGCRISYNSRTK 190
           + A DR++R G W+   +F LG K+SGKR+GIVGLG IG  VAKRLE FGC ISYNSR++
Sbjct: 127 IPAADRYVRSGNWAKFGDFQLGSKVSGKRVGIVGLGSIGSFVAKRLESFGCVISYNSRSQ 186

Query: 191 KPLVPYSYYSNVHELAAECEALIICCGLTEETRHMINREVMVALGKDGVIINIGRGAIVD 250
           K   PY YYS++  LA   + L++CC LT+ET H++NREVM  LGKDGV+IN+GRG ++D
Sbjct: 187 KQSSPYRYYSDILSLAENNDVLVLCCSLTDETHHIVNREVMELLGKDGVVINVGRGKLID 246

Query: 251 EKEMIRCLLQGEIWGAGLDVFENEPEIPEQLFTLDNVVLSPHVAVTTHETFLRLSKLMVD 310
           EKEM++CL+ G I GAGLDVFENEP +P++LF LDNVVLSPH AV T  +   ++++ + 
Sbjct: 247 EKEMVKCLVDGVIGGAGLDVFENEPAVPQELFGLDNVVLSPHFAVATPGSLDNVAQIALA 306

Query: 311 NLEAFFSNRPLVSPL 325
           NL+AFFSNRPL+SP+
Sbjct: 307 NLKAFFSNRPLLSPV 320

[Close]

BLAST of Moc05g03320 vs. TAIR 10
Match: AT1G79870.1 (D-isomer specific 2-hydroxyacid dehydrogenase family protein )

HSP 1 Score: 260.8 bits (665), Expect = 1.5e-69
Identity = 137/312 (43.91%), Postives = 203/312 (65.06%), Query Frame = 0


Query: 13  VLVLGPPTVFSCLESQFPNRFHFLKPWLSKLPLPQFLTSYAQYVQALIIPGGSLALTSAI 72
           VL++ P  + S LE++   RF+ L+ W S  P    L    +     ++   S    + +
Sbjct: 6   VLMMCP--MSSYLENELEKRFNLLRFWTS--PEKSVLLETHRNSIRAVVGNASAGADAQL 65

Query: 73  LDCLPSLKLVATASAGVDHLDLPELRRRGVAVAYAGNLFSEDVADMAVGLLIDVLRKVSA 132
           +  LP+L++V++ S G+D +DL + + +G+ V    ++ +EDVAD+A+GL++ +LR++  
Sbjct: 66  ISDLPNLEIVSSFSVGLDKIDLGKCKEKGIRVTNTPDVLTEDVADLAIGLILALLRRLCE 125

Query: 133 GDRFMRQGLWSTKVNFPLGLKLSGKRIGIVGLGRIGCEVAKRLEGFGCRISYNSRTKKPL 192
            DR++R G W  +  F L  K SGK +GI+GLGRIG  +AKR E F C I+Y SRT KP 
Sbjct: 126 CDRYVRSGKWK-QGEFQLTTKFSGKSVGIIGLGRIGTAIAKRAEAFSCPINYYSRTIKPD 185

Query: 193 VPYSYYSNVHELAAECEALIICCGLTEETRHMINREVMVALGKDGVIINIGRGAIVDEKE 252
           V Y YY  V +LA   + L++ C LTE+TRH+++R+VM ALG  GV+INIGRG  VDE+E
Sbjct: 186 VAYKYYPTVVDLAQNSDILVVACPLTEQTRHIVDRQVMDALGAKGVLINIGRGPHVDEQE 245

Query: 253 MIRCLLQGEIWGAGLDVFENEPEIPEQLFTLDNVVLSPHVAVTTHETFLRLSKLMVDNLE 312
           +I+ L +G + GA LDVFE EP +PE+LF L+NVVL PHV   T ET   ++ L+V NLE
Sbjct: 246 LIKALTEGRLGGAALDVFEQEPHVPEELFGLENVVLLPHVGSGTVETRNAMADLVVGNLE 305

Query: 313 AFFSNRPLVSPL 325
           A FS + L++P+
Sbjct: 306 AHFSGKSLLTPV 312

[Close]

BLAST of Moc05g03320 vs. TAIR 10
Match: AT1G79870.2 (D-isomer specific 2-hydroxyacid dehydrogenase family protein )

HSP 1 Score: 234.2 bits (596), Expect = 1.5e-61
Identity = 127/312 (40.71%), Postives = 190/312 (60.90%), Query Frame = 0


Query: 13  VLVLGPPTVFSCLESQFPNRFHFLKPWLSKLPLPQFLTSYAQYVQALIIPGGSLALTSAI 72
           VL++ P  + S LE++   RF+ L+ W S  P    L    +     ++   S    + +
Sbjct: 6   VLMMCP--MSSYLENELEKRFNLLRFWTS--PEKSVLLETHRNSIRAVVGNASAGADAQL 65

Query: 73  LDCLPSLKLVATASAGVDHLDLPELRRRGVAVAYAGNLFSEDVADMAVGLLIDVLRKVSA 132
           +  LP+L++V++ S G+D +DL + + +G+ V    ++ +EDVAD+A+GL++ +LR++  
Sbjct: 66  ISDLPNLEIVSSFSVGLDKIDLGKCKEKGIRVTNTPDVLTEDVADLAIGLILALLRRLCE 125

Query: 133 GDRFMRQGLWSTKVNFPLGLKLSGKRIGIVGLGRIGCEVAKRLEGFGCRISYNSRTKKPL 192
            DR++R G W                      GRIG  +AKR E F C I+Y SRT KP 
Sbjct: 126 CDRYVRSGKWKQ--------------------GRIGTAIAKRAEAFSCPINYYSRTIKPD 185

Query: 193 VPYSYYSNVHELAAECEALIICCGLTEETRHMINREVMVALGKDGVIINIGRGAIVDEKE 252
           V Y YY  V +LA   + L++ C LTE+TRH+++R+VM ALG  GV+INIGRG  VDE+E
Sbjct: 186 VAYKYYPTVVDLAQNSDILVVACPLTEQTRHIVDRQVMDALGAKGVLINIGRGPHVDEQE 245

Query: 253 MIRCLLQGEIWGAGLDVFENEPEIPEQLFTLDNVVLSPHVAVTTHETFLRLSKLMVDNLE 312
           +I+ L +G + GA LDVFE EP +PE+LF L+NVVL PHV   T ET   ++ L+V NLE
Sbjct: 246 LIKALTEGRLGGAALDVFEQEPHVPEELFGLENVVLLPHVGSGTVETRNAMADLVVGNLE 293

Query: 313 AFFSNRPLVSPL 325
           A FS + L++P+
Sbjct: 306 AHFSGKSLLTPV 293

[Close]

BLAST of Moc05g03320 vs. TAIR 10
Match: AT2G45630.1 (D-isomer specific 2-hydroxyacid dehydrogenase family protein )

HSP 1 Score: 138.7 bits (348), Expect = 8.8e-33
Identity = 74/156 (47.44%), Postives = 105/156 (67.31%), Query Frame = 0


Query: 1   MAPESQDQDLPQVLVLGPPTVFSCLESQF--PNRFHFLKPWLSKLPLPQFLTSYAQYVQA 60
           MA  S  + LP+VL++  P   + L   F    +F  LK + S LPLP+FL  ++  + A
Sbjct: 12  MATSSSTEKLPRVLIVKRPDAMAVLGDGFVASTKFEILKAFESPLPLPEFLAYHSDSISA 71

Query: 61  LIIPGGSLALTSAILDCLPSLKLVATASAGVDHLDLPELRRRGVAVAYAGNLFSEDVADM 120
           +I P  +  +T+ ++  LP+L+LV T SAGVDH+DL E RRRG++VA AG+ FSEDVAD 
Sbjct: 72  IIAPVAA-PVTADLIRILPNLRLVVTTSAGVDHVDLVECRRRGISVANAGSSFSEDVADT 131

Query: 121 AVGLLIDVLRKVSAGDRFMRQGLWSTKVNFPLGLKL 155
           AVGLLIDV R++SA +RF++Q  W  K ++PLG K+
Sbjct: 132 AVGLLIDVFRRISAANRFVKQRFWPLKGDYPLGSKV 166

The following BLAST results are available for this feature:

BLAST of Moc05g03320 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022147257.1	3.4e-188	100.00	glyoxylate/hydroxypyruvate reductase HPR3-like [Momordica charantia]	[more]
XP_022159358.1	2.9e-147	81.10	glyoxylate/hydroxypyruvate reductase HPR3-like [Momordica charantia]	[more]
KAG6597182.1	1.5e-146	81.88	Glyoxylate/hydroxypyruvate reductase HPR3, partial [Cucurbita argyrosperma subsp...	[more]
XP_022147048.1	2.0e-143	80.25	glyoxylate/hydroxypyruvate reductase HPR3-like [Momordica charantia]	[more]
XP_023526607.1	1.7e-142	79.01	glyoxylate/hydroxypyruvate reductase HPR3-like [Cucurbita pepo subsp. pepo]	[more]

BLAST of Moc05g03320 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9LE33	8.4e-89	51.75	Glyoxylate/hydroxypyruvate reductase HPR3 OS=Arabidopsis thaliana OX=3702 GN=HPR...	[more]
Q65CJ7	2.7e-71	45.19	Hydroxyphenylpyruvate reductase OS=Plectranthus scutellarioides OX=4142 GN=HPPR ...	[more]
A5CAL1	7.9e-71	43.71	Glyoxylate/hydroxypyruvate/pyruvate reductase 2KGR OS=Vitis vinifera OX=29760 GN...	[more]
Q9CA90	2.2e-68	43.91	Glyoxylate/hydroxypyruvate reductase A HPR2 OS=Arabidopsis thaliana OX=3702 GN=H...	[more]
Q5FTU6	6.3e-52	36.71	2-ketogluconate reductase OS=Gluconobacter oxydans (strain 621H) OX=290633 GN=GO...	[more]

BLAST of Moc05g03320 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1D1V9	1.7e-188	100.00	glyoxylate/hydroxypyruvate reductase HPR3-like OS=Momordica charantia OX=3673 GN...	[more]
A0A6J1DYK8	1.4e-147	81.10	glyoxylate/hydroxypyruvate reductase HPR3-like OS=Momordica charantia OX=3673 GN...	[more]
A0A6J1D073	9.5e-144	80.25	glyoxylate/hydroxypyruvate reductase HPR3-like OS=Momordica charantia OX=3673 GN...	[more]
A0A6J1EEZ0	5.2e-142	79.32	glyoxylate/hydroxypyruvate reductase HPR3-like OS=Cucurbita moschata OX=3662 GN=...	[more]
A0A0A0L908	2.2e-140	78.95	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G124900 PE=3 SV=1	[more]

BLAST of Moc05g03320 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G45630.2	1.7e-97	54.74	D-isomer specific 2-hydroxyacid dehydrogenase family protein	[more]
AT1G12550.1	6.0e-90	51.75	D-isomer specific 2-hydroxyacid dehydrogenase family protein	[more]
AT1G79870.1	1.5e-69	43.91	D-isomer specific 2-hydroxyacid dehydrogenase family protein	[more]
AT1G79870.2	1.5e-61	40.71	D-isomer specific 2-hydroxyacid dehydrogenase family protein	[more]
AT2G45630.1	8.8e-33	47.44	D-isomer specific 2-hydroxyacid dehydrogenase family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (OHB3-1) v2
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	GENE3D	3.40.50.720		coord: 29..319 e-value: 4.0E-103 score: 346.1
None	No IPR available	GENE3D	3.40.50.720		coord: 112..296 e-value: 4.0E-103 score: 346.1
None	No IPR available	PANTHER	PTHR10996	2-HYDROXYACID DEHYDROGENASE-RELATED	coord: 7..324
None	No IPR available	PANTHER	PTHR10996:SF179	D-ISOMER SPECIFIC 2-HYDROXYACID DEHYDROGENASE FAMILY PROTEIN-RELATED	coord: 7..324
None	No IPR available	CDD	cd12156	HPPR	coord: 11..316 e-value: 1.36658E-144 score: 408.395
None	No IPR available	SUPERFAMILY	52283	Formate/glycerate dehydrogenase catalytic domain-like	coord: 13..147
IPR006140	D-isomer specific 2-hydroxyacid dehydrogenase, NAD-binding domain	PFAM	PF02826	2-Hacid_dh_C	coord: 120..293 e-value: 6.0E-49 score: 165.8
IPR006139	D-isomer specific 2-hydroxyacid dehydrogenase, catalytic domain	PFAM	PF00389	2-Hacid_dh	coord: 55..320 e-value: 1.0E-16 score: 61.6
IPR036291	NAD(P)-binding domain superfamily	SUPERFAMILY	51735	NAD(P)-binding Rossmann-fold domains	coord: 112..293

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Moc05g03320.1	Moc05g03320.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0005829	cytosol
molecular_function	GO:0030267	glyoxylate reductase (NADP+) activity
molecular_function	GO:0016618	hydroxypyruvate reductase activity
molecular_function	GO:0051287	NAD binding
molecular_function	GO:0016616	oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor