Moc05g02900 (gene) Bitter gourd (OHB3-1) v2

BLAST of Moc05g02900 vs. NCBI nr
Match: XP_022147136.1 (pectinesterase 2 [Momordica charantia])

HSP 1 Score: 1067.8 bits (2760), Expect = 3.1e-308
Identity = 539/539 (100.00%), Postives = 539/539 (100.00%), Query Frame = 0

Query: 1   MRTQAPQKSHISSRTQKKKRKRKMATIKIRQSLSVVALVVVLVFPSAIFGYSEKEIKSWC 60
           MRTQAPQKSHISSRTQKKKRKRKMATIKIRQSLSVVALVVVLVFPSAIFGYSEKEIKSWC
Sbjct: 1   MRTQAPQKSHISSRTQKKKRKRKMATIKIRQSLSVVALVVVLVFPSAIFGYSEKEIKSWC 60

Query: 61  SQTPYPAPCEHFLRTKAATSNNSPITDKSHFLKILVETALSTAVSAHKDAISLGPKCRNS 120
           SQTPYPAPCEHFLRTKAATSNNSPITDKSHFLKILVETALSTAVSAHKDAISLGPKCRNS
Sbjct: 61  SQTPYPAPCEHFLRTKAATSNNSPITDKSHFLKILVETALSTAVSAHKDAISLGPKCRNS 120

Query: 121 REKSAWADCVYLYDQIISRLNRTADRCSRSDAQTWLSAALTALETCRTGFDELGVSASGY 180
           REKSAWADCVYLYDQIISRLNRTADRCSRSDAQTWLSAALTALETCRTGFDELGVSASGY
Sbjct: 121 REKSAWADCVYLYDQIISRLNRTADRCSRSDAQTWLSAALTALETCRTGFDELGVSASGY 180

Query: 181 PLTANNVSKLISDGLSVNGPPSPDGFGPPSRVDGFPVWVSPGDRKLLQSGSPAGNADVVV 240
           PLTANNVSKLISDGLSVNGPPSPDGFGPPSRVDGFPVWVSPGDRKLLQSGSPAGNADVVV
Sbjct: 181 PLTANNVSKLISDGLSVNGPPSPDGFGPPSRVDGFPVWVSPGDRKLLQSGSPAGNADVVV 240

Query: 241 AKDGSGDFQTVAAAVAAAKGGGRFVIYVKAGVYSENVEIKAKNVMLVGDGIGKTVITGSR 300
           AKDGSGDFQTVAAAVAAAKGGGRFVIYVKAGVYSENVEIKAKNVMLVGDGIGKTVITGSR
Sbjct: 241 AKDGSGDFQTVAAAVAAAKGGGRFVIYVKAGVYSENVEIKAKNVMLVGDGIGKTVITGSR 300

Query: 301 SVGGGSTTFRSATVAVDGDGFIARDITFRNTAGAKNHQAVALRSGSDLSVFYRCGFEGFQ 360
           SVGGGSTTFRSATVAVDGDGFIARDITFRNTAGAKNHQAVALRSGSDLSVFYRCGFEGFQ
Sbjct: 301 SVGGGSTTFRSATVAVDGDGFIARDITFRNTAGAKNHQAVALRSGSDLSVFYRCGFEGFQ 360

Query: 361 DTLYVYAERQFYKQCDIYGTVDFIFGNAAVVIQDSNIIARDPPNKTITLTAQGRSDPNQN 420
           DTLYVYAERQFYKQCDIYGTVDFIFGNAAVVIQDSNIIARDPPNKTITLTAQGRSDPNQN
Sbjct: 361 DTLYVYAERQFYKQCDIYGTVDFIFGNAAVVIQDSNIIARDPPNKTITLTAQGRSDPNQN 420

Query: 421 TGISIHNCRVTSAGGLSGVKAYLGRPWRQYSRTVFMKSNIGGFINPAGWMEWSGNFALNT 480
           TGISIHNCRVTSAGGLSGVKAYLGRPWRQYSRTVFMKSNIGGFINPAGWMEWSGNFALNT
Sbjct: 421 TGISIHNCRVTSAGGLSGVKAYLGRPWRQYSRTVFMKSNIGGFINPAGWMEWSGNFALNT 480

Query: 481 LYYGEYSNTGPGSSTANRVKWKGYHVITSAAEASKFTVGNFIAGGSWLPNTGVPFTSGL 540
           LYYGEYSNTGPGSSTANRVKWKGYHVITSAAEASKFTVGNFIAGGSWLPNTGVPFTSGL
Sbjct: 481 LYYGEYSNTGPGSSTANRVKWKGYHVITSAAEASKFTVGNFIAGGSWLPNTGVPFTSGL 539

BLAST of Moc05g02900 vs. NCBI nr
Match: XP_038877776.1 (pectinesterase 2 [Benincasa hispida])

HSP 1 Score: 871.3 bits (2250), Expect = 4.3e-249
Identity = 434/505 (85.94%), Postives = 466/505 (92.28%), Query Frame = 0

Query: 35  VVALVVVLVFPSAIFGYSEKEIKSWCSQTPYPAPCEHFLRTKAATSNNSPITDKSHFLKI 94
           +V +V+VL+FP  I+ YSE EIK+WCSQTPYPAPCE FL+TK AT+  SPI  KSHF +I
Sbjct: 8   LVVVVIVLIFPLDIYCYSEIEIKNWCSQTPYPAPCEEFLKTK-ATTKRSPIATKSHFFEI 67

Query: 95  LVETALSTAVSAHKDAISLGPKCRNSREKSAWADCVYLYDQIISRLNRTADRCSRSDAQT 154
           LVETAL  AVSA ++A+SLGPKCRNS+E++AW DCV LYDQII+RLNRT DRC+  DAQT
Sbjct: 68  LVETALERAVSARQNALSLGPKCRNSKERTAWTDCVDLYDQIITRLNRTVDRCTPVDAQT 127

Query: 155 WLSAALTALETCRTGFDELGVSASGYPLTANNVSKLISDGLSVNGPPSPDGFGPPSRVDG 214
           WLSAALTALETCRTG +ELGVSA GYPLTANNVSKLISDGLSVN P SP+GF PP+  DG
Sbjct: 128 WLSAALTALETCRTGLEELGVSAFGYPLTANNVSKLISDGLSVNKPASPEGFEPPTMEDG 187

Query: 215 FPVWVSPGDRKLLQSGSPAGNADVVVAKDGSGDFQTVAAAVAAAKGGGRFVIYVKAGVYS 274
           FP WV PGDR+LLQSGSP   A+VVVA+DGSG+F+TV  AVAAAKGGGRFVIYVKAGVYS
Sbjct: 188 FPTWVRPGDRRLLQSGSP--KANVVVAQDGSGNFKTVKEAVAAAKGGGRFVIYVKAGVYS 247

Query: 275 ENVEIKAKNVMLVGDGIGKTVITGSRSVGGGSTTFRSATVAVDGDGFIARDITFRNTAGA 334
           ENVEI AKNVML+GDGIGKTVITGSRSVGGGSTTFRSATVAVDGDGFIARDITFRNTAGA
Sbjct: 248 ENVEINAKNVMLIGDGIGKTVITGSRSVGGGSTTFRSATVAVDGDGFIARDITFRNTAGA 307

Query: 335 KNHQAVALRSGSDLSVFYRCGFEGFQDTLYVYAERQFYKQCDIYGTVDFIFGNAAVVIQD 394
           KNHQAVALRSGSDLSVFYRCGFEGFQDTLYVYAERQFYKQCDIYGTVDFIFGNAAVVIQD
Sbjct: 308 KNHQAVALRSGSDLSVFYRCGFEGFQDTLYVYAERQFYKQCDIYGTVDFIFGNAAVVIQD 367

Query: 395 SNIIARDPPNKTITLTAQGRSDPNQNTGISIHNCRVTSAGGLSGVKAYLGRPWRQYSRTV 454
            NIIARDPPNKTIT+TAQGRSDPNQNTGISIHNCR+TSAGGLSGVKAYLGRPWRQYSRTV
Sbjct: 368 CNIIARDPPNKTITVTAQGRSDPNQNTGISIHNCRITSAGGLSGVKAYLGRPWRQYSRTV 427

Query: 455 FMKSNIGGFINPAGWMEWSGNFALNTLYYGEYSNTGPGSSTANRVKWKGYHVITSAAEAS 514
            MKS+IGGFI+PAGWMEWSGNFALNTLYYGEY NTGPG+STANRVKWKGY VITSAAEAS
Sbjct: 428 VMKSSIGGFISPAGWMEWSGNFALNTLYYGEYMNTGPGASTANRVKWKGYRVITSAAEAS 487

Query: 515 KFTVGNFIAGGSWLPNTGVPFTSGL 540
           KFTVGNFIAGGSWLP+TGVPFTSGL
Sbjct: 488 KFTVGNFIAGGSWLPSTGVPFTSGL 509

BLAST of Moc05g02900 vs. NCBI nr
Match: XP_008438193.1 (PREDICTED: pectinesterase 2 [Cucumis melo] >KAA0049055.1 pectinesterase 2 [Cucumis melo var. makuwa])

HSP 1 Score: 870.5 bits (2248), Expect = 7.3e-249
Identity = 429/511 (83.95%), Postives = 473/511 (92.56%), Query Frame = 0

Query: 30  RQSLSVVALVVVLVFPSAIFGYSEKEIKSWCSQTPYPAPCEHFLRTKAATSNNSPITDKS 89
           R S+  + ++VVL+FP  +  YSEKEIK+WCSQTPYPAPCE FL+TKA+T  ++PIT KS
Sbjct: 3   RHSIVFLVVLVVLIFPWDVLCYSEKEIKNWCSQTPYPAPCEEFLKTKASTKKSTPITTKS 62

Query: 90  HFLKILVETALSTAVSAHKDAISLGPKCRNSREKSAWADCVYLYDQIISRLNRTADRCSR 149
           HF +ILVETAL  AVSAHK+A+SLGPKCRNS+EK+AW DCV LYD+II+RLNRT+DRC+ 
Sbjct: 63  HFFEILVETALERAVSAHKNALSLGPKCRNSKEKTAWTDCVDLYDEIITRLNRTSDRCTP 122

Query: 150 SDAQTWLSAALTALETCRTGFDELGVSASGYPLTANNVSKLISDGLSVNGPPSPDGFGPP 209
           +DAQTWLSAALTALETCRTGFDELG+SA GYPLTANNVSKLISDGLSVN P SP+G+ P 
Sbjct: 123 ADAQTWLSAALTALETCRTGFDELGLSAFGYPLTANNVSKLISDGLSVNKPASPEGYEPT 182

Query: 210 SRV-DGFPVWVSPGDRKLLQSGSPAGNADVVVAKDGSGDFQTVAAAVAAAKGGGRFVIYV 269
           + + DGFP WV PGDRKLLQSGSP   A+VVVA+DGSG+F+TV  AVAAAKGGGRFVIYV
Sbjct: 183 TIMDDGFPTWVRPGDRKLLQSGSP--KANVVVAQDGSGNFKTVKEAVAAAKGGGRFVIYV 242

Query: 270 KAGVYSENVEIKAKNVMLVGDGIGKTVITGSRSVGGGSTTFRSATVAVDGDGFIARDITF 329
           K+GVY+EN++IKAKNVM+VGDGIGKT+ITGSRSVGGGSTTFRSATVAVDGDGFIARDITF
Sbjct: 243 KSGVYNENLDIKAKNVMMVGDGIGKTIITGSRSVGGGSTTFRSATVAVDGDGFIARDITF 302

Query: 330 RNTAGAKNHQAVALRSGSDLSVFYRCGFEGFQDTLYVYAERQFYKQCDIYGTVDFIFGNA 389
           RNTAGAKNHQAVALRSGSDLSVFYRCGFEG+QDTLYVYAERQFYKQCDIYGTVDFIFGNA
Sbjct: 303 RNTAGAKNHQAVALRSGSDLSVFYRCGFEGYQDTLYVYAERQFYKQCDIYGTVDFIFGNA 362

Query: 390 AVVIQDSNIIARDPPNKTITLTAQGRSDPNQNTGISIHNCRVTSAGGLSGVKAYLGRPWR 449
           AVV+QD NIIARDPPNKTIT+TAQGRSDPNQNTGISIHNCR+TSAGGLSGVKAYLGRPWR
Sbjct: 363 AVVLQDCNIIARDPPNKTITVTAQGRSDPNQNTGISIHNCRITSAGGLSGVKAYLGRPWR 422

Query: 450 QYSRTVFMKSNIGGFINPAGWMEWSGNFALNTLYYGEYSNTGPGSSTANRVKWKGYHVIT 509
           QYSRTV MKS+IGGFI+PAGWM WSGNFALNTLYYGEY NTGPG+STANRV WKGY VIT
Sbjct: 423 QYSRTVVMKSSIGGFISPAGWMPWSGNFALNTLYYGEYMNTGPGASTANRVNWKGYRVIT 482

Query: 510 SAAEASKFTVGNFIAGGSWLPNTGVPFTSGL 540
           SAAEASKFTVGNFIAGGSWLP+TGVPFTSGL
Sbjct: 483 SAAEASKFTVGNFIAGGSWLPSTGVPFTSGL 511

BLAST of Moc05g02900 vs. NCBI nr
Match: TYK17508.1 (pectinesterase 2 [Cucumis melo var. makuwa])

HSP 1 Score: 867.8 bits (2241), Expect = 4.8e-248
Identity = 428/511 (83.76%), Postives = 472/511 (92.37%), Query Frame = 0

Query: 30  RQSLSVVALVVVLVFPSAIFGYSEKEIKSWCSQTPYPAPCEHFLRTKAATSNNSPITDKS 89
           R S+  + ++VVL+FP  +  YSEKEIK+WCSQTPYPAPCE FL+TKA+T  ++PIT KS
Sbjct: 3   RHSIVFLIVLVVLIFPWDVLCYSEKEIKNWCSQTPYPAPCEEFLKTKASTKKSTPITTKS 62

Query: 90  HFLKILVETALSTAVSAHKDAISLGPKCRNSREKSAWADCVYLYDQIISRLNRTADRCSR 149
           HF +ILVETAL  AVSAHK+A+SLGPKCRNS+EK+AW DCV LYD+II+RLNRT+DRC+ 
Sbjct: 63  HFFEILVETALERAVSAHKNALSLGPKCRNSKEKTAWTDCVDLYDEIITRLNRTSDRCTP 122

Query: 150 SDAQTWLSAALTALETCRTGFDELGVSASGYPLTANNVSKLISDGLSVNGPPSPDGFGPP 209
           +DAQTWLSAALTALETCRTGFDELG+SA GYPLTANNVSKLISDGLSVN P SP+G+ P 
Sbjct: 123 ADAQTWLSAALTALETCRTGFDELGLSAFGYPLTANNVSKLISDGLSVNKPASPEGYEPT 182

Query: 210 SRV-DGFPVWVSPGDRKLLQSGSPAGNADVVVAKDGSGDFQTVAAAVAAAKGGGRFVIYV 269
           + + DGFP WV PGDRKLLQSGSP   A+VVVA+DGSG+F+TV  AVAAAK GGRFVIYV
Sbjct: 183 TIMDDGFPTWVRPGDRKLLQSGSP--KANVVVAQDGSGNFKTVKEAVAAAKSGGRFVIYV 242

Query: 270 KAGVYSENVEIKAKNVMLVGDGIGKTVITGSRSVGGGSTTFRSATVAVDGDGFIARDITF 329
           K+GVY+EN++IKAKNVM+VGDGIGKT+ITGSRSVGGGSTTFRSATVAVDGDGFIARDITF
Sbjct: 243 KSGVYNENLDIKAKNVMMVGDGIGKTIITGSRSVGGGSTTFRSATVAVDGDGFIARDITF 302

Query: 330 RNTAGAKNHQAVALRSGSDLSVFYRCGFEGFQDTLYVYAERQFYKQCDIYGTVDFIFGNA 389
           RNTAGAKNHQAVALRSGSDLSVFYRCGFEG+QDTLYVYAERQFYKQCDIYGTVDFIFGNA
Sbjct: 303 RNTAGAKNHQAVALRSGSDLSVFYRCGFEGYQDTLYVYAERQFYKQCDIYGTVDFIFGNA 362

Query: 390 AVVIQDSNIIARDPPNKTITLTAQGRSDPNQNTGISIHNCRVTSAGGLSGVKAYLGRPWR 449
           AVV+QD NIIARDPPNKTIT+TAQGRSDPNQNTGISIHNCR+TSAGGLSGVKAYLGRPWR
Sbjct: 363 AVVLQDCNIIARDPPNKTITVTAQGRSDPNQNTGISIHNCRITSAGGLSGVKAYLGRPWR 422

Query: 450 QYSRTVFMKSNIGGFINPAGWMEWSGNFALNTLYYGEYSNTGPGSSTANRVKWKGYHVIT 509
           QYSRTV MKS+IGGFI+PAGWM WSGNFALNTLYYGEY NTGPG+STANRV WKGY VIT
Sbjct: 423 QYSRTVVMKSSIGGFISPAGWMPWSGNFALNTLYYGEYMNTGPGASTANRVNWKGYRVIT 482

Query: 510 SAAEASKFTVGNFIAGGSWLPNTGVPFTSGL 540
           SAAEASKFTVGNFIAGGSWLP+TGVPFTSGL
Sbjct: 483 SAAEASKFTVGNFIAGGSWLPSTGVPFTSGL 511

BLAST of Moc05g02900 vs. NCBI nr
Match: XP_004133929.1 (pectinesterase 2 [Cucumis sativus] >KGN56617.1 hypothetical protein Csa_010246 [Cucumis sativus])

HSP 1 Score: 865.1 bits (2234), Expect = 3.1e-247
Identity = 425/510 (83.33%), Postives = 470/510 (92.16%), Query Frame = 0

Query: 30  RQSLSVVALVVVLVFPSAIFGYSEKEIKSWCSQTPYPAPCEHFLRTKAATSNNSPITDKS 89
           R ++    +++VL+FPS I  YSEKEIK+WCSQTPYPAPCE FL+TK AT+  +PIT KS
Sbjct: 3   RHTIVFFVVLIVLIFPSDILCYSEKEIKNWCSQTPYPAPCEEFLKTK-ATTKKTPITTKS 62

Query: 90  HFLKILVETALSTAVSAHKDAISLGPKCRNSREKSAWADCVYLYDQIISRLNRTADRCSR 149
           HF +ILVETAL  AVSAHK+A+SLGPKCRNS+EK+AW DCV LYDQII+RLNRT+ RCS 
Sbjct: 63  HFFEILVETALERAVSAHKNALSLGPKCRNSKEKTAWTDCVDLYDQIITRLNRTSARCSP 122

Query: 150 SDAQTWLSAALTALETCRTGFDELGVSASGYPLTANNVSKLISDGLSVNGPPSPDGFGPP 209
           +DAQTWLSAALTALETCRTGF+ELG+SA GYPLTANNVSKLISDGLSVN P SP+G+ P 
Sbjct: 123 ADAQTWLSAALTALETCRTGFEELGLSAFGYPLTANNVSKLISDGLSVNKPASPEGYEPT 182

Query: 210 SRVDGFPVWVSPGDRKLLQSGSPAGNADVVVAKDGSGDFQTVAAAVAAAKGGGRFVIYVK 269
           +  DGFP WVSPG+RKLLQS SP   ADVVVA+DGSG+F+TV  A++AAKGGGRFVIY+K
Sbjct: 183 TMTDGFPTWVSPGNRKLLQSESP--KADVVVAQDGSGNFKTVKDAISAAKGGGRFVIYIK 242

Query: 270 AGVYSENVEIKAKNVMLVGDGIGKTVITGSRSVGGGSTTFRSATVAVDGDGFIARDITFR 329
           +GVY+EN++IKAKNVM+VGDGIGKT+ITGSRSVGGGSTTFRSATVAVDGDGFIARDITFR
Sbjct: 243 SGVYNENLDIKAKNVMMVGDGIGKTIITGSRSVGGGSTTFRSATVAVDGDGFIARDITFR 302

Query: 330 NTAGAKNHQAVALRSGSDLSVFYRCGFEGFQDTLYVYAERQFYKQCDIYGTVDFIFGNAA 389
           NTAGAKNHQAVALRSGSDLSVFYRCGFEG+QDTLYVYAERQFYKQCDIYGTVDFIFGNAA
Sbjct: 303 NTAGAKNHQAVALRSGSDLSVFYRCGFEGYQDTLYVYAERQFYKQCDIYGTVDFIFGNAA 362

Query: 390 VVIQDSNIIARDPPNKTITLTAQGRSDPNQNTGISIHNCRVTSAGGLSGVKAYLGRPWRQ 449
           VV+QD NIIARDPPNKTITLTAQGRSDPNQNTGISIHNCR+TS+GGLSGVKAYLGRPWRQ
Sbjct: 363 VVLQDCNIIARDPPNKTITLTAQGRSDPNQNTGISIHNCRITSSGGLSGVKAYLGRPWRQ 422

Query: 450 YSRTVFMKSNIGGFINPAGWMEWSGNFALNTLYYGEYSNTGPGSSTANRVKWKGYHVITS 509
           YSRTV MKS+IGGFI+PAGWM WSGNFALNTLYY EY NTGPG+STANRV WKGYHVITS
Sbjct: 423 YSRTVVMKSSIGGFISPAGWMPWSGNFALNTLYYAEYMNTGPGASTANRVNWKGYHVITS 482

Query: 510 AAEASKFTVGNFIAGGSWLPNTGVPFTSGL 540
           A+EASKFTVGNFIAGGSWLP+TGVPFTSGL
Sbjct: 483 ASEASKFTVGNFIAGGSWLPSTGVPFTSGL 509

BLAST of Moc05g02900 vs. ExPASy Swiss-Prot
Match: O04887 (Pectinesterase 2 OS=Citrus sinensis OX=2711 GN=PECS-2.1 PE=2 SV=1)

HSP 1 Score: 641.7 bits (1654), Expect = 7.3e-183
Identity = 323/514 (62.84%), Postives = 393/514 (76.46%), Query Frame = 0

Query: 35  VVALVVVLVFPSAIFGYSEKEIKSWCSQTPYPAPCEHFLRTKAATSNNSPITDKSHFLKI 94
           +   +V+       FGYS +E+KSWC +TP P PCE+FL  K   ++    TD   F KI
Sbjct: 7   ITVSLVLFSLSHTSFGYSPEEVKSWCGKTPNPQPCEYFLTQKTDVTSIKQDTD---FYKI 66

Query: 95  LVETALSTAVSAHKDAISLGPKCRNSREKSAWADCVYLYDQIISRLNRTADR---CSRSD 154
            ++ AL  A +A     +LG KCRN REK+AW DC  LY+  + +LN+T++    C++ D
Sbjct: 67  SLQLALERATTAQSRTYTLGSKCRNEREKAAWEDCRELYELTVLKLNQTSNSSPGCTKVD 126

Query: 155 AQTWLSAALTALETCRTGFDELGVSASGYPLTANNVSKLISDGLSVNGPPSPDGFGPPSR 214
            QTWLS ALT LETCR   ++LGV     PL +NNV+KLIS+ LS+N  P    +  PS 
Sbjct: 127 KQTWLSTALTNLETCRASLEDLGVPEYVLPLLSNNVTKLISNTLSLNKVP----YNEPSY 186

Query: 215 VDGFPVWVSPGDRKLLQSGSPAGNADVVVAKDGSGDFQTVAAAVAAAK--GGGRFVIYVK 274
            DGFP WV PGDRKLLQ+      A++VVA+DGSG+ +T+  AVAAA   GG R+VIY+K
Sbjct: 187 KDGFPTWVKPGDRKLLQT---TPRANIVVAQDGSGNVKTIQEAVAAASRAGGSRYVIYIK 246

Query: 275 AGVYSENVEIKAKNVMLVGDGIGKTVITGSRSVGGGSTTFRSATVAVDGDGFIARDITFR 334
           AG Y+EN+E+K KN+M VGDGIGKT+ITGS+SVGGG+TTF+SATVAV GD FIARDIT R
Sbjct: 247 AGTYNENIEVKLKNIMFVGDGIGKTIITGSKSVGGGATTFKSATVAVVGDNFIARDITIR 306

Query: 335 NTAGAKNHQAVALRSGSDLSVFYRCGFEGFQDTLYVYAERQFYKQCDIYGTVDFIFGNAA 394
           NTAG  NHQAVALRSGSDLSVFYRC FEG+QDTLYV+++RQFY++CDIYGTVDFIFGNAA
Sbjct: 307 NTAGPNNHQAVALRSGSDLSVFYRCSFEGYQDTLYVHSQRQFYRECDIYGTVDFIFGNAA 366

Query: 395 VVIQDSNIIARDPPNKTITLTAQGRSDPNQNTGISIHNCRVTSAGGL----SGVKAYLGR 454
           VV+Q+ NI AR PPN+T TLTAQGR+DPNQ+TGI IHNCRVT+A  L    S VK +LGR
Sbjct: 367 VVLQNCNIFARKPPNRTNTLTAQGRTDPNQSTGIIIHNCRVTAASDLKPVQSSVKTFLGR 426

Query: 455 PWRQYSRTVFMKSNIGGFINPAGWMEWSGNFALNTLYYGEYSNTGPGSSTANRVKWKGYH 514
           PW+QYSRTV++K+ +   INPAGWMEWSG+FALNTLYY EY NTGPGSSTANRVKW+GYH
Sbjct: 427 PWKQYSRTVYIKTFLDSLINPAGWMEWSGDFALNTLYYAEYMNTGPGSSTANRVKWRGYH 486

Query: 515 VITSAAEASKFTVGNFIAGGSWLPNTGVPFTSGL 540
           V+TS ++ S+FTVGNFIAG SWLP T VPFTSGL
Sbjct: 487 VLTSPSQVSQFTVGNFIAGNSWLPATNVPFTSGL 510

BLAST of Moc05g02900 vs. ExPASy Swiss-Prot
Match: O22149 (Probable pectinesterase/pectinesterase inhibitor 17 OS=Arabidopsis thaliana OX=3702 GN=PME17 PE=2 SV=2)

HSP 1 Score: 639.0 bits (1647), Expect = 4.7e-182
Identity = 322/509 (63.26%), Postives = 394/509 (77.41%), Query Frame = 0

Query: 38  LVVVLVFPSAIFGYSEKEIKSWCSQTPYPAPCEHFLRTKAATSNNSPITDKSHFLKILVE 97
           +++ ++  S + GY++K++K+WCSQTP P PCE+FL      SNN PI  +S FLKI ++
Sbjct: 12  VILCILVASTVSGYNQKDVKAWCSQTPNPKPCEYFL---THNSNNEPIKSESEFLKISMK 71

Query: 98  TALSTAVSAHKDAISLGPKCRNSREKSAWADCVYLYDQIISRLNRTAD---RCSRSDAQT 157
             L  A+ A   A +LGPKCR++REK+AW DC+ LYD  +S++N T D   +CS+ DAQT
Sbjct: 72  LVLDRAILAKTHAFTLGPKCRDTREKAAWEDCIKLYDLTVSKINETMDPNVKCSKLDAQT 131

Query: 158 WLSAALTALETCRTGFDELGVSASGYPLTANNVSKLISDGLSVNGPPSPDGFGPPSRVDG 217
           WLS ALT L+TCR GF ELGV+    PL +NNVS L+ + L++N    P  + PP + DG
Sbjct: 132 WLSTALTNLDTCRAGFLELGVTDIVLPLMSNNVSNLLCNTLAIN--KVPFNYTPPEK-DG 191

Query: 218 FPVWVSPGDRKLLQSGSPAGNADVVVAKDGSGDFQTVAAAVAAAKGGGRFVIYVKAGVYS 277
           FP WV PGDRKLLQS +P  NA  VVAKDGSG+F+T+  A+ AA G GRFVIYVK GVYS
Sbjct: 192 FPSWVKPGDRKLLQSSTPKDNA--VVAKDGSGNFKTIKEAIDAASGSGRFVIYVKQGVYS 251

Query: 278 ENVEIKAKNVMLVGDGIGKTVITGSRSVGGGSTTFRSATVAVDGDGFIARDITFRNTAGA 337
           EN+EI+ KNVML GDGIGKT+ITGS+SVGGG+TTF SATVA  GDGFIAR ITFRNTAGA
Sbjct: 252 ENLEIRKKNVMLRGDGIGKTIITGSKSVGGGTTTFNSATVAAVGDGFIARGITFRNTAGA 311

Query: 338 KNHQAVALRSGSDLSVFYRCGFEGFQDTLYVYAERQFYKQCDIYGTVDFIFGNAAVVIQD 397
            N QAVALRSGSDLSVFY+C FE +QDTLYV++ RQFY+ CD+YGTVDFIFGNAA V+Q+
Sbjct: 312 SNEQAVALRSGSDLSVFYQCSFEAYQDTLYVHSNRQFYRDCDVYGTVDFIFGNAAAVLQN 371

Query: 398 SNIIARDPPNKTITLTAQGRSDPNQNTGISIHNCRVTSAGGLSGV----KAYLGRPWRQY 457
            NI AR P +KT T+TAQGRSDPNQNTGI IHN RVT+A  L  V    K YLGRPWRQY
Sbjct: 372 CNIFARRPRSKTNTITAQGRSDPNQNTGIIIHNSRVTAASDLRPVLGSTKTYLGRPWRQY 431

Query: 458 SRTVFMKSNIGGFINPAGWMEWSGNFALNTLYYGEYSNTGPGSSTANRVKWKGYHVITSA 517
           SRTVFMK+++   I+P GW+EW GNFAL TL+Y E+ NTGPG+ST+ RV W G+ V+ SA
Sbjct: 432 SRTVFMKTSLDSLIDPRGWLEWDGNFALKTLFYAEFQNTGPGASTSGRVTWPGFRVLGSA 491

Query: 518 AEASKFTVGNFIAGGSWLPNTGVPFTSGL 540
           +EASKFTVG F+AGGSW+P++ VPFTSGL
Sbjct: 492 SEASKFTVGTFLAGGSWIPSS-VPFTSGL 511

BLAST of Moc05g02900 vs. ExPASy Swiss-Prot
Match: Q9STY3 (Probable pectinesterase/pectinesterase inhibitor 33 OS=Arabidopsis thaliana OX=3702 GN=PME33 PE=2 SV=1)

HSP 1 Score: 454.5 bits (1168), Expect = 1.6e-126
Identity = 253/508 (49.80%), Postives = 331/508 (65.16%), Query Frame = 0

Query: 59  WCSQTPYPAPCEHFLRTKAATS-NNSPITDKSHFLKILVETALSTAVSAHKDAISLGPKC 118
           WC++TP+   C ++ R  +  + N  P   +S FL++LV+ AL  AV  H   +  GP C
Sbjct: 87  WCNKTPHAETCNYYFRKSSQNNINLRPPRFRSEFLRMLVKVALDQAVITHSQTVKFGPSC 146

Query: 119 RNSREKSAWADCVYLYDQIISRLNRTAD----------RCSRSDAQTWLSAALTALETCR 178
            N++ K+AW+DCV L+   +++LNRT            +C+  DAQTWLS A T +ETCR
Sbjct: 147 TNNQRKAAWSDCVNLFQNTVAQLNRTLKGLNPAASSDVKCTDFDAQTWLSTAQTNIETCR 206

Query: 179 TGFDELGVSASGYPLTAN-NVSKLISDGLSVNG--PPSPDGFGPPSRVDGFPVWVSPGDR 238
           +G ++L VS    P+ +N N+S LI + L+VNG      D     +  + FP WVS  +R
Sbjct: 207 SGSEDLNVSDFVMPVISNKNLSDLIGNCLAVNGVLMKQHDHTTTANHKEYFPSWVSRHER 266

Query: 239 KLLQSGSPA-GNADVVVAKDGSGDFQTVAAAV---AAAKGGGRFVIYVKAGVYSENVEI- 298
           +LL S S A  +  +VVA+D SG F+++ AA+   A  +   RFVIYVK GVY EN+++ 
Sbjct: 267 RLLVSASLAKSSPHLVVAQDRSGHFRSIQAAINFAARRRFKSRFVIYVKKGVYRENIDVG 326

Query: 299 -KAKNVMLVGDGIGKTVITGSRSVGGGSTTFRSATVAVDGDGFIARDITFRNTAGAKNHQ 358
               N+MLVGDG  KT+IT  RSV  G TT+ SAT    G  F+A+D+TF NTAG    Q
Sbjct: 327 NDNHNIMLVGDGERKTIITSGRSVQHGYTTYNSATGGFGGQRFVAKDMTFINTAGPLRGQ 386

Query: 359 AVALRSGSDLSVFYRCGFEGFQDTLYVYAERQFYKQCDIYGTVDFIFGNAAVVIQDSNII 418
           AVA+RS SDLSVFYR G  GFQDTLY++++RQF+++C I GT+DFIFGNAAVV Q+  I+
Sbjct: 387 AVAVRSSSDLSVFYRVGIHGFQDTLYIHSQRQFFRECYISGTIDFIFGNAAVVFQNCMIL 446

Query: 419 ARDP-PNKTITLTAQGRSDPNQNTGISIHNCRVTSAGGLSGV----KAYLGRPWRQYSRT 478
            R P   +   +TAQGR DP QNTGI+IH+ R+ +A  L  V    K YLGRPW+ YSR 
Sbjct: 447 VRRPLHGQANIITAQGRGDPFQNTGITIHSSRIIAASDLKPVIRAYKTYLGRPWQAYSRV 506

Query: 479 VFMKSNIGGFINPAGWMEW--SGNFALNTLYYGEYSNTGPGSSTANRVKWKGYHVITSAA 538
             MK+ I   I+P GW  W    NFALNT++YGEY N GPGSST  RV+WKG+H ITS A
Sbjct: 507 TIMKTYIDNSISPLGWSPWLRGSNFALNTVFYGEYKNFGPGSSTRWRVRWKGFHAITSTA 566

Query: 539 EASKFTVGNFIAGGSWLPNTGVPFTSGL 540
            AS+FTVG+ IAGGSWLP TGVPF SGL
Sbjct: 567 VASRFTVGSLIAGGSWLPATGVPFKSGL 594

BLAST of Moc05g02900 vs. ExPASy Swiss-Prot
Match: Q9FHN5 (Probable pectinesterase/pectinesterase inhibitor 59 OS=Arabidopsis thaliana OX=3702 GN=PME59 PE=2 SV=1)

HSP 1 Score: 454.5 bits (1168), Expect = 1.6e-126
Identity = 256/533 (48.03%), Postives = 339/533 (63.60%), Query Frame = 0

Query: 33  LSVVALVVVLVFPSAIFGYSEKEIKSWCSQTPYPAPCEHFLRTKAATSNNSPITDKSHFL 92
           L ++ LV++ V P  +   +++    WC +TPYP PC+ + +     +     T  S F 
Sbjct: 13  LHLILLVLLCVHP--LTTVADRNSTDWCDKTPYPDPCKCYFKNH---NGFQQPTQLSEFR 72

Query: 93  KILVETALSTAVSAHKDAISLGPKCRNSREKSAWADCVYLYDQIISRLNRT--------- 152
            +LVE A+  A+SA  +  + G  C +S++++  ADC+ LY   I +LNRT         
Sbjct: 73  VMLVEAAMDRAISARAELTNSGKNCTDSKKQAVLADCIDLYGDTIMQLNRTLHGVSPKAG 132

Query: 153 -ADRCSRSDAQTWLSAALTALETCRTGFDELGVSASGYPLTAN-NVSKLISDGLSVNGPP 212
            A  C+  DAQTWLS ALT  ETCR G  +L V+    P+ +N  +S LIS+ L+VNG  
Sbjct: 133 AAKSCTDFDAQTWLSTALTNTETCRRGSSDLNVTDFITPIVSNTKISHLISNCLAVNGAL 192

Query: 213 SPDG--FGPPSRVDGFPVWVSPGDRKLLQSGSPAGNADVVVAKDGSGDFQTVAAAVAAA- 272
              G      +   GFP W+S  D++LL+    A  A++VVAKDGSG F TV AA+  A 
Sbjct: 193 LTAGNKGNTTANQKGFPTWLSRKDKRLLR----AVRANLVVAKDGSGHFNTVQAAIDVAG 252

Query: 273 ---KGGGRFVIYVKAGVYSENVEIKAKN--VMLVGDGIGKTVITGSRSVGGGSTTFRSAT 332
                 GRFVIYVK G+Y EN+ ++  N  +MLVGDG+  T+ITG RSV GG TT+ SAT
Sbjct: 253 RRKVTSGRFVIYVKRGIYQENINVRLNNDDIMLVGDGMRSTIITGGRSVQGGYTTYNSAT 312

Query: 333 VAVDGDGFIARDITFRNTAGAKNHQAVALRSGSDLSVFYRCGFEGFQDTLYVYAERQFYK 392
             ++G  FIA+ ITFRNTAG    QAVALRS SDLS+FY+C  EG+QDTL V+++RQFY+
Sbjct: 313 AGIEGLHFIAKGITFRNTAGPAKGQAVALRSSSDLSIFYKCSIEGYQDTLMVHSQRQFYR 372

Query: 393 QCDIYGTVDFIFGNAAVVIQDSNIIARDP-PNKTITLTAQGRSDPNQNTGISIHNCRVTS 452
           +C IYGTVDFIFGNAA V Q+  I+ R P   +   +TAQGR+DP QNTGISIHN R+  
Sbjct: 373 ECYIYGTVDFIFGNAAAVFQNCLILPRRPLKGQANVITAQGRADPFQNTGISIHNSRILP 432

Query: 453 AGGL----SGVKAYLGRPWRQYSRTVFMKSNIGGFINPAGWMEW--SGNFALNTLYYGEY 512
           A  L      VK Y+GRPW ++SRTV +++ +   ++P GW  W     F L+TL+Y EY
Sbjct: 433 APDLKPVVGTVKTYMGRPWMKFSRTVVLQTYLDNVVSPVGWSPWIEGSVFGLDTLFYAEY 492

Query: 513 SNTGPGSSTANRVKWKGYHVITSAAEASKFTVGNFIAGGSWLPNTGVPFTSGL 540
            NTGP SST  RV WKG+HV+  A++AS FTVG FIAG +WLP TG+PFTSGL
Sbjct: 493 KNTGPASSTRWRVSWKGFHVLGRASDASAFTVGKFIAGTAWLPRTGIPFTSGL 536

BLAST of Moc05g02900 vs. ExPASy Swiss-Prot
Match: O81320 (Putative pectinesterase/pectinesterase inhibitor 38 OS=Arabidopsis thaliana OX=3702 GN=PME38 PE=3 SV=1)

HSP 1 Score: 442.6 bits (1137), Expect = 6.5e-123
Identity = 247/494 (50.00%), Postives = 314/494 (63.56%), Query Frame = 0

Query: 60  CSQTPYPAPCEHFLRTKAATSNNSPITDKSHFLKILVETALSTAVSAHKDAISLGPKCRN 119
           C +TP+P  C      K     + PI   + FL++ VE  L  AV A  D   L P+  +
Sbjct: 8   CDETPHPGEC------KTLLIKHKPIRSTTQFLQVSVERTLDGAVKAKSDTYFLEPQFGS 67

Query: 120 SREKSAWADCVYLYDQIISRLNRTA----DRCSRSDAQTWLSAALTALETCRTGFDELGV 179
              K AW +C+ LY+Q I RLN +     + CSRSD Q WLS ALT L+TC+    ELGV
Sbjct: 68  ---KQAWEECMDLYEQTIHRLNESVLCPKNVCSRSDVQAWLSTALTNLDTCQEEMSELGV 127

Query: 180 SASGYPLTANNVSKLISDGLSVNGPPSPDG--FGPPSRVDGFPVWVSPGDRKLLQSGSPA 239
           S+     +  +++  + + L++N     +G  FG           +S    K L  G   
Sbjct: 128 SSH----SLESITIDVINTLAINKRMEQNGKEFG-----------ISKITMKTLSIGE-- 187

Query: 240 GNADVVVAKDGSGDFQTVAAAVAAA----KGGGRFVIYVKAGVYSE--NVEIKAKNVMLV 299
              DVVVA+DGSGD++T+  AV  A    KG  R+VI+VK GVY E  NV IK+ N+M+ 
Sbjct: 188 -KVDVVVAQDGSGDYKTIQEAVNGAGERLKGSPRYVIHVKQGVYEEYVNVGIKSNNIMIT 247

Query: 300 GDGIGKTVITGSRSVGGGSTTFRSATVAVDGDGFIARDITFRNTAGAKNHQAVALRSGSD 359
           GDGIGKT+ITG +S G G +T++SAT   +GDGF+ RDIT RNTAG +NHQAVALRS SD
Sbjct: 248 GDGIGKTIITGDKSKGRGFSTYKSATFVAEGDGFVGRDITIRNTAGPENHQAVALRSNSD 307

Query: 360 LSVFYRCGFEGFQDTLYVYAERQFYKQCDIYGTVDFIFGNAAVVIQDSNIIARDPPNKTI 419
           +SVFYRC  EG+QDTLYV++ RQF+++CDIYGTVDFIFGNAA V+Q+  I AR+PPN   
Sbjct: 308 MSVFYRCSIEGYQDTLYVHSGRQFFRECDIYGTVDFIFGNAAAVLQNCRIFARNPPNGVN 367

Query: 420 TLTAQGRSDPNQNTGISIHNCRVTSAGG--LSGVKAYLGRPWRQYSRTVFMKSNIGGFIN 479
           T+TAQ R +PNQ TGI IHN  V  A G  L GVK YLGRPWR Y+RTV + + +   I 
Sbjct: 368 TITAQSRFNPNQTTGIVIHNSVVKGAPGVQLGGVKTYLGRPWRSYARTVVIGTYLDTLIE 427

Query: 480 PAGWMEWSGNFALNTLYYGEYSNTGPGSSTANRVKWKGYHVITSAAEASKFTVGNFIAGG 539
           P GW++W    AL+TLYYGEY N+GPGS T NRV W G+HVI+   EA +FT+  FI   
Sbjct: 428 PNGWIDWDNVTALSTLYYGEYQNSGPGSGTENRVDWAGFHVISDIQEAREFTLPKFIDSA 474

BLAST of Moc05g02900 vs. ExPASy TrEMBL
Match: A0A6J1D1F9 (Pectinesterase OS=Momordica charantia OX=3673 GN=LOC111016139 PE=3 SV=1)

HSP 1 Score: 1067.8 bits (2760), Expect = 1.5e-308
Identity = 539/539 (100.00%), Postives = 539/539 (100.00%), Query Frame = 0

Query: 1   MRTQAPQKSHISSRTQKKKRKRKMATIKIRQSLSVVALVVVLVFPSAIFGYSEKEIKSWC 60
           MRTQAPQKSHISSRTQKKKRKRKMATIKIRQSLSVVALVVVLVFPSAIFGYSEKEIKSWC
Sbjct: 1   MRTQAPQKSHISSRTQKKKRKRKMATIKIRQSLSVVALVVVLVFPSAIFGYSEKEIKSWC 60

Query: 61  SQTPYPAPCEHFLRTKAATSNNSPITDKSHFLKILVETALSTAVSAHKDAISLGPKCRNS 120
           SQTPYPAPCEHFLRTKAATSNNSPITDKSHFLKILVETALSTAVSAHKDAISLGPKCRNS
Sbjct: 61  SQTPYPAPCEHFLRTKAATSNNSPITDKSHFLKILVETALSTAVSAHKDAISLGPKCRNS 120

Query: 121 REKSAWADCVYLYDQIISRLNRTADRCSRSDAQTWLSAALTALETCRTGFDELGVSASGY 180
           REKSAWADCVYLYDQIISRLNRTADRCSRSDAQTWLSAALTALETCRTGFDELGVSASGY
Sbjct: 121 REKSAWADCVYLYDQIISRLNRTADRCSRSDAQTWLSAALTALETCRTGFDELGVSASGY 180

Query: 181 PLTANNVSKLISDGLSVNGPPSPDGFGPPSRVDGFPVWVSPGDRKLLQSGSPAGNADVVV 240
           PLTANNVSKLISDGLSVNGPPSPDGFGPPSRVDGFPVWVSPGDRKLLQSGSPAGNADVVV
Sbjct: 181 PLTANNVSKLISDGLSVNGPPSPDGFGPPSRVDGFPVWVSPGDRKLLQSGSPAGNADVVV 240

Query: 241 AKDGSGDFQTVAAAVAAAKGGGRFVIYVKAGVYSENVEIKAKNVMLVGDGIGKTVITGSR 300
           AKDGSGDFQTVAAAVAAAKGGGRFVIYVKAGVYSENVEIKAKNVMLVGDGIGKTVITGSR
Sbjct: 241 AKDGSGDFQTVAAAVAAAKGGGRFVIYVKAGVYSENVEIKAKNVMLVGDGIGKTVITGSR 300

Query: 301 SVGGGSTTFRSATVAVDGDGFIARDITFRNTAGAKNHQAVALRSGSDLSVFYRCGFEGFQ 360
           SVGGGSTTFRSATVAVDGDGFIARDITFRNTAGAKNHQAVALRSGSDLSVFYRCGFEGFQ
Sbjct: 301 SVGGGSTTFRSATVAVDGDGFIARDITFRNTAGAKNHQAVALRSGSDLSVFYRCGFEGFQ 360

Query: 361 DTLYVYAERQFYKQCDIYGTVDFIFGNAAVVIQDSNIIARDPPNKTITLTAQGRSDPNQN 420
           DTLYVYAERQFYKQCDIYGTVDFIFGNAAVVIQDSNIIARDPPNKTITLTAQGRSDPNQN
Sbjct: 361 DTLYVYAERQFYKQCDIYGTVDFIFGNAAVVIQDSNIIARDPPNKTITLTAQGRSDPNQN 420

Query: 421 TGISIHNCRVTSAGGLSGVKAYLGRPWRQYSRTVFMKSNIGGFINPAGWMEWSGNFALNT 480
           TGISIHNCRVTSAGGLSGVKAYLGRPWRQYSRTVFMKSNIGGFINPAGWMEWSGNFALNT
Sbjct: 421 TGISIHNCRVTSAGGLSGVKAYLGRPWRQYSRTVFMKSNIGGFINPAGWMEWSGNFALNT 480

Query: 481 LYYGEYSNTGPGSSTANRVKWKGYHVITSAAEASKFTVGNFIAGGSWLPNTGVPFTSGL 540
           LYYGEYSNTGPGSSTANRVKWKGYHVITSAAEASKFTVGNFIAGGSWLPNTGVPFTSGL
Sbjct: 481 LYYGEYSNTGPGSSTANRVKWKGYHVITSAAEASKFTVGNFIAGGSWLPNTGVPFTSGL 539

BLAST of Moc05g02900 vs. ExPASy TrEMBL
Match: A0A5A7U603 (Pectinesterase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold171G002830 PE=3 SV=1)

HSP 1 Score: 870.5 bits (2248), Expect = 3.6e-249
Identity = 429/511 (83.95%), Postives = 473/511 (92.56%), Query Frame = 0

Query: 30  RQSLSVVALVVVLVFPSAIFGYSEKEIKSWCSQTPYPAPCEHFLRTKAATSNNSPITDKS 89
           R S+  + ++VVL+FP  +  YSEKEIK+WCSQTPYPAPCE FL+TKA+T  ++PIT KS
Sbjct: 3   RHSIVFLVVLVVLIFPWDVLCYSEKEIKNWCSQTPYPAPCEEFLKTKASTKKSTPITTKS 62

Query: 90  HFLKILVETALSTAVSAHKDAISLGPKCRNSREKSAWADCVYLYDQIISRLNRTADRCSR 149
           HF +ILVETAL  AVSAHK+A+SLGPKCRNS+EK+AW DCV LYD+II+RLNRT+DRC+ 
Sbjct: 63  HFFEILVETALERAVSAHKNALSLGPKCRNSKEKTAWTDCVDLYDEIITRLNRTSDRCTP 122

Query: 150 SDAQTWLSAALTALETCRTGFDELGVSASGYPLTANNVSKLISDGLSVNGPPSPDGFGPP 209
           +DAQTWLSAALTALETCRTGFDELG+SA GYPLTANNVSKLISDGLSVN P SP+G+ P 
Sbjct: 123 ADAQTWLSAALTALETCRTGFDELGLSAFGYPLTANNVSKLISDGLSVNKPASPEGYEPT 182

Query: 210 SRV-DGFPVWVSPGDRKLLQSGSPAGNADVVVAKDGSGDFQTVAAAVAAAKGGGRFVIYV 269
           + + DGFP WV PGDRKLLQSGSP   A+VVVA+DGSG+F+TV  AVAAAKGGGRFVIYV
Sbjct: 183 TIMDDGFPTWVRPGDRKLLQSGSP--KANVVVAQDGSGNFKTVKEAVAAAKGGGRFVIYV 242

Query: 270 KAGVYSENVEIKAKNVMLVGDGIGKTVITGSRSVGGGSTTFRSATVAVDGDGFIARDITF 329
           K+GVY+EN++IKAKNVM+VGDGIGKT+ITGSRSVGGGSTTFRSATVAVDGDGFIARDITF
Sbjct: 243 KSGVYNENLDIKAKNVMMVGDGIGKTIITGSRSVGGGSTTFRSATVAVDGDGFIARDITF 302

Query: 330 RNTAGAKNHQAVALRSGSDLSVFYRCGFEGFQDTLYVYAERQFYKQCDIYGTVDFIFGNA 389
           RNTAGAKNHQAVALRSGSDLSVFYRCGFEG+QDTLYVYAERQFYKQCDIYGTVDFIFGNA
Sbjct: 303 RNTAGAKNHQAVALRSGSDLSVFYRCGFEGYQDTLYVYAERQFYKQCDIYGTVDFIFGNA 362

Query: 390 AVVIQDSNIIARDPPNKTITLTAQGRSDPNQNTGISIHNCRVTSAGGLSGVKAYLGRPWR 449
           AVV+QD NIIARDPPNKTIT+TAQGRSDPNQNTGISIHNCR+TSAGGLSGVKAYLGRPWR
Sbjct: 363 AVVLQDCNIIARDPPNKTITVTAQGRSDPNQNTGISIHNCRITSAGGLSGVKAYLGRPWR 422

Query: 450 QYSRTVFMKSNIGGFINPAGWMEWSGNFALNTLYYGEYSNTGPGSSTANRVKWKGYHVIT 509
           QYSRTV MKS+IGGFI+PAGWM WSGNFALNTLYYGEY NTGPG+STANRV WKGY VIT
Sbjct: 423 QYSRTVVMKSSIGGFISPAGWMPWSGNFALNTLYYGEYMNTGPGASTANRVNWKGYRVIT 482

Query: 510 SAAEASKFTVGNFIAGGSWLPNTGVPFTSGL 540
           SAAEASKFTVGNFIAGGSWLP+TGVPFTSGL
Sbjct: 483 SAAEASKFTVGNFIAGGSWLPSTGVPFTSGL 511

BLAST of Moc05g02900 vs. ExPASy TrEMBL
Match: A0A1S3AVW4 (Pectinesterase OS=Cucumis melo OX=3656 GN=LOC103483370 PE=3 SV=1)

HSP 1 Score: 870.5 bits (2248), Expect = 3.6e-249
Identity = 429/511 (83.95%), Postives = 473/511 (92.56%), Query Frame = 0

Query: 30  RQSLSVVALVVVLVFPSAIFGYSEKEIKSWCSQTPYPAPCEHFLRTKAATSNNSPITDKS 89
           R S+  + ++VVL+FP  +  YSEKEIK+WCSQTPYPAPCE FL+TKA+T  ++PIT KS
Sbjct: 3   RHSIVFLVVLVVLIFPWDVLCYSEKEIKNWCSQTPYPAPCEEFLKTKASTKKSTPITTKS 62

Query: 90  HFLKILVETALSTAVSAHKDAISLGPKCRNSREKSAWADCVYLYDQIISRLNRTADRCSR 149
           HF +ILVETAL  AVSAHK+A+SLGPKCRNS+EK+AW DCV LYD+II+RLNRT+DRC+ 
Sbjct: 63  HFFEILVETALERAVSAHKNALSLGPKCRNSKEKTAWTDCVDLYDEIITRLNRTSDRCTP 122

Query: 150 SDAQTWLSAALTALETCRTGFDELGVSASGYPLTANNVSKLISDGLSVNGPPSPDGFGPP 209
           +DAQTWLSAALTALETCRTGFDELG+SA GYPLTANNVSKLISDGLSVN P SP+G+ P 
Sbjct: 123 ADAQTWLSAALTALETCRTGFDELGLSAFGYPLTANNVSKLISDGLSVNKPASPEGYEPT 182

Query: 210 SRV-DGFPVWVSPGDRKLLQSGSPAGNADVVVAKDGSGDFQTVAAAVAAAKGGGRFVIYV 269
           + + DGFP WV PGDRKLLQSGSP   A+VVVA+DGSG+F+TV  AVAAAKGGGRFVIYV
Sbjct: 183 TIMDDGFPTWVRPGDRKLLQSGSP--KANVVVAQDGSGNFKTVKEAVAAAKGGGRFVIYV 242

Query: 270 KAGVYSENVEIKAKNVMLVGDGIGKTVITGSRSVGGGSTTFRSATVAVDGDGFIARDITF 329
           K+GVY+EN++IKAKNVM+VGDGIGKT+ITGSRSVGGGSTTFRSATVAVDGDGFIARDITF
Sbjct: 243 KSGVYNENLDIKAKNVMMVGDGIGKTIITGSRSVGGGSTTFRSATVAVDGDGFIARDITF 302

Query: 330 RNTAGAKNHQAVALRSGSDLSVFYRCGFEGFQDTLYVYAERQFYKQCDIYGTVDFIFGNA 389
           RNTAGAKNHQAVALRSGSDLSVFYRCGFEG+QDTLYVYAERQFYKQCDIYGTVDFIFGNA
Sbjct: 303 RNTAGAKNHQAVALRSGSDLSVFYRCGFEGYQDTLYVYAERQFYKQCDIYGTVDFIFGNA 362

Query: 390 AVVIQDSNIIARDPPNKTITLTAQGRSDPNQNTGISIHNCRVTSAGGLSGVKAYLGRPWR 449
           AVV+QD NIIARDPPNKTIT+TAQGRSDPNQNTGISIHNCR+TSAGGLSGVKAYLGRPWR
Sbjct: 363 AVVLQDCNIIARDPPNKTITVTAQGRSDPNQNTGISIHNCRITSAGGLSGVKAYLGRPWR 422

Query: 450 QYSRTVFMKSNIGGFINPAGWMEWSGNFALNTLYYGEYSNTGPGSSTANRVKWKGYHVIT 509
           QYSRTV MKS+IGGFI+PAGWM WSGNFALNTLYYGEY NTGPG+STANRV WKGY VIT
Sbjct: 423 QYSRTVVMKSSIGGFISPAGWMPWSGNFALNTLYYGEYMNTGPGASTANRVNWKGYRVIT 482

Query: 510 SAAEASKFTVGNFIAGGSWLPNTGVPFTSGL 540
           SAAEASKFTVGNFIAGGSWLP+TGVPFTSGL
Sbjct: 483 SAAEASKFTVGNFIAGGSWLPSTGVPFTSGL 511

BLAST of Moc05g02900 vs. ExPASy TrEMBL
Match: A0A5D3D1Y4 (Pectinesterase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold434G003750 PE=3 SV=1)

HSP 1 Score: 867.8 bits (2241), Expect = 2.3e-248
Identity = 428/511 (83.76%), Postives = 472/511 (92.37%), Query Frame = 0

Query: 30  RQSLSVVALVVVLVFPSAIFGYSEKEIKSWCSQTPYPAPCEHFLRTKAATSNNSPITDKS 89
           R S+  + ++VVL+FP  +  YSEKEIK+WCSQTPYPAPCE FL+TKA+T  ++PIT KS
Sbjct: 3   RHSIVFLIVLVVLIFPWDVLCYSEKEIKNWCSQTPYPAPCEEFLKTKASTKKSTPITTKS 62

Query: 90  HFLKILVETALSTAVSAHKDAISLGPKCRNSREKSAWADCVYLYDQIISRLNRTADRCSR 149
           HF +ILVETAL  AVSAHK+A+SLGPKCRNS+EK+AW DCV LYD+II+RLNRT+DRC+ 
Sbjct: 63  HFFEILVETALERAVSAHKNALSLGPKCRNSKEKTAWTDCVDLYDEIITRLNRTSDRCTP 122

Query: 150 SDAQTWLSAALTALETCRTGFDELGVSASGYPLTANNVSKLISDGLSVNGPPSPDGFGPP 209
           +DAQTWLSAALTALETCRTGFDELG+SA GYPLTANNVSKLISDGLSVN P SP+G+ P 
Sbjct: 123 ADAQTWLSAALTALETCRTGFDELGLSAFGYPLTANNVSKLISDGLSVNKPASPEGYEPT 182

Query: 210 SRV-DGFPVWVSPGDRKLLQSGSPAGNADVVVAKDGSGDFQTVAAAVAAAKGGGRFVIYV 269
           + + DGFP WV PGDRKLLQSGSP   A+VVVA+DGSG+F+TV  AVAAAK GGRFVIYV
Sbjct: 183 TIMDDGFPTWVRPGDRKLLQSGSP--KANVVVAQDGSGNFKTVKEAVAAAKSGGRFVIYV 242

Query: 270 KAGVYSENVEIKAKNVMLVGDGIGKTVITGSRSVGGGSTTFRSATVAVDGDGFIARDITF 329
           K+GVY+EN++IKAKNVM+VGDGIGKT+ITGSRSVGGGSTTFRSATVAVDGDGFIARDITF
Sbjct: 243 KSGVYNENLDIKAKNVMMVGDGIGKTIITGSRSVGGGSTTFRSATVAVDGDGFIARDITF 302

Query: 330 RNTAGAKNHQAVALRSGSDLSVFYRCGFEGFQDTLYVYAERQFYKQCDIYGTVDFIFGNA 389
           RNTAGAKNHQAVALRSGSDLSVFYRCGFEG+QDTLYVYAERQFYKQCDIYGTVDFIFGNA
Sbjct: 303 RNTAGAKNHQAVALRSGSDLSVFYRCGFEGYQDTLYVYAERQFYKQCDIYGTVDFIFGNA 362

Query: 390 AVVIQDSNIIARDPPNKTITLTAQGRSDPNQNTGISIHNCRVTSAGGLSGVKAYLGRPWR 449
           AVV+QD NIIARDPPNKTIT+TAQGRSDPNQNTGISIHNCR+TSAGGLSGVKAYLGRPWR
Sbjct: 363 AVVLQDCNIIARDPPNKTITVTAQGRSDPNQNTGISIHNCRITSAGGLSGVKAYLGRPWR 422

Query: 450 QYSRTVFMKSNIGGFINPAGWMEWSGNFALNTLYYGEYSNTGPGSSTANRVKWKGYHVIT 509
           QYSRTV MKS+IGGFI+PAGWM WSGNFALNTLYYGEY NTGPG+STANRV WKGY VIT
Sbjct: 423 QYSRTVVMKSSIGGFISPAGWMPWSGNFALNTLYYGEYMNTGPGASTANRVNWKGYRVIT 482

Query: 510 SAAEASKFTVGNFIAGGSWLPNTGVPFTSGL 540
           SAAEASKFTVGNFIAGGSWLP+TGVPFTSGL
Sbjct: 483 SAAEASKFTVGNFIAGGSWLPSTGVPFTSGL 511

BLAST of Moc05g02900 vs. ExPASy TrEMBL
Match: A0A0A0L7N4 (Pectinesterase OS=Cucumis sativus OX=3659 GN=Csa_3G126810 PE=3 SV=1)

HSP 1 Score: 865.1 bits (2234), Expect = 1.5e-247
Identity = 425/510 (83.33%), Postives = 470/510 (92.16%), Query Frame = 0

Query: 30  RQSLSVVALVVVLVFPSAIFGYSEKEIKSWCSQTPYPAPCEHFLRTKAATSNNSPITDKS 89
           R ++    +++VL+FPS I  YSEKEIK+WCSQTPYPAPCE FL+TK AT+  +PIT KS
Sbjct: 3   RHTIVFFVVLIVLIFPSDILCYSEKEIKNWCSQTPYPAPCEEFLKTK-ATTKKTPITTKS 62

Query: 90  HFLKILVETALSTAVSAHKDAISLGPKCRNSREKSAWADCVYLYDQIISRLNRTADRCSR 149
           HF +ILVETAL  AVSAHK+A+SLGPKCRNS+EK+AW DCV LYDQII+RLNRT+ RCS 
Sbjct: 63  HFFEILVETALERAVSAHKNALSLGPKCRNSKEKTAWTDCVDLYDQIITRLNRTSARCSP 122

Query: 150 SDAQTWLSAALTALETCRTGFDELGVSASGYPLTANNVSKLISDGLSVNGPPSPDGFGPP 209
           +DAQTWLSAALTALETCRTGF+ELG+SA GYPLTANNVSKLISDGLSVN P SP+G+ P 
Sbjct: 123 ADAQTWLSAALTALETCRTGFEELGLSAFGYPLTANNVSKLISDGLSVNKPASPEGYEPT 182

Query: 210 SRVDGFPVWVSPGDRKLLQSGSPAGNADVVVAKDGSGDFQTVAAAVAAAKGGGRFVIYVK 269
           +  DGFP WVSPG+RKLLQS SP   ADVVVA+DGSG+F+TV  A++AAKGGGRFVIY+K
Sbjct: 183 TMTDGFPTWVSPGNRKLLQSESP--KADVVVAQDGSGNFKTVKDAISAAKGGGRFVIYIK 242

Query: 270 AGVYSENVEIKAKNVMLVGDGIGKTVITGSRSVGGGSTTFRSATVAVDGDGFIARDITFR 329
           +GVY+EN++IKAKNVM+VGDGIGKT+ITGSRSVGGGSTTFRSATVAVDGDGFIARDITFR
Sbjct: 243 SGVYNENLDIKAKNVMMVGDGIGKTIITGSRSVGGGSTTFRSATVAVDGDGFIARDITFR 302

Query: 330 NTAGAKNHQAVALRSGSDLSVFYRCGFEGFQDTLYVYAERQFYKQCDIYGTVDFIFGNAA 389
           NTAGAKNHQAVALRSGSDLSVFYRCGFEG+QDTLYVYAERQFYKQCDIYGTVDFIFGNAA
Sbjct: 303 NTAGAKNHQAVALRSGSDLSVFYRCGFEGYQDTLYVYAERQFYKQCDIYGTVDFIFGNAA 362

Query: 390 VVIQDSNIIARDPPNKTITLTAQGRSDPNQNTGISIHNCRVTSAGGLSGVKAYLGRPWRQ 449
           VV+QD NIIARDPPNKTITLTAQGRSDPNQNTGISIHNCR+TS+GGLSGVKAYLGRPWRQ
Sbjct: 363 VVLQDCNIIARDPPNKTITLTAQGRSDPNQNTGISIHNCRITSSGGLSGVKAYLGRPWRQ 422

Query: 450 YSRTVFMKSNIGGFINPAGWMEWSGNFALNTLYYGEYSNTGPGSSTANRVKWKGYHVITS 509
           YSRTV MKS+IGGFI+PAGWM WSGNFALNTLYY EY NTGPG+STANRV WKGYHVITS
Sbjct: 423 YSRTVVMKSSIGGFISPAGWMPWSGNFALNTLYYAEYMNTGPGASTANRVNWKGYHVITS 482

Query: 510 AAEASKFTVGNFIAGGSWLPNTGVPFTSGL 540
           A+EASKFTVGNFIAGGSWLP+TGVPFTSGL
Sbjct: 483 ASEASKFTVGNFIAGGSWLPSTGVPFTSGL 509

BLAST of Moc05g02900 vs. TAIR 10
Match: AT2G45220.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 639.0 bits (1647), Expect = 3.3e-183
Identity = 322/509 (63.26%), Postives = 394/509 (77.41%), Query Frame = 0

Query: 38  LVVVLVFPSAIFGYSEKEIKSWCSQTPYPAPCEHFLRTKAATSNNSPITDKSHFLKILVE 97
           +++ ++  S + GY++K++K+WCSQTP P PCE+FL      SNN PI  +S FLKI ++
Sbjct: 12  VILCILVASTVSGYNQKDVKAWCSQTPNPKPCEYFL---THNSNNEPIKSESEFLKISMK 71

Query: 98  TALSTAVSAHKDAISLGPKCRNSREKSAWADCVYLYDQIISRLNRTAD---RCSRSDAQT 157
             L  A+ A   A +LGPKCR++REK+AW DC+ LYD  +S++N T D   +CS+ DAQT
Sbjct: 72  LVLDRAILAKTHAFTLGPKCRDTREKAAWEDCIKLYDLTVSKINETMDPNVKCSKLDAQT 131

Query: 158 WLSAALTALETCRTGFDELGVSASGYPLTANNVSKLISDGLSVNGPPSPDGFGPPSRVDG 217
           WLS ALT L+TCR GF ELGV+    PL +NNVS L+ + L++N    P  + PP + DG
Sbjct: 132 WLSTALTNLDTCRAGFLELGVTDIVLPLMSNNVSNLLCNTLAIN--KVPFNYTPPEK-DG 191

Query: 218 FPVWVSPGDRKLLQSGSPAGNADVVVAKDGSGDFQTVAAAVAAAKGGGRFVIYVKAGVYS 277
           FP WV PGDRKLLQS +P  NA  VVAKDGSG+F+T+  A+ AA G GRFVIYVK GVYS
Sbjct: 192 FPSWVKPGDRKLLQSSTPKDNA--VVAKDGSGNFKTIKEAIDAASGSGRFVIYVKQGVYS 251

Query: 278 ENVEIKAKNVMLVGDGIGKTVITGSRSVGGGSTTFRSATVAVDGDGFIARDITFRNTAGA 337
           EN+EI+ KNVML GDGIGKT+ITGS+SVGGG+TTF SATVA  GDGFIAR ITFRNTAGA
Sbjct: 252 ENLEIRKKNVMLRGDGIGKTIITGSKSVGGGTTTFNSATVAAVGDGFIARGITFRNTAGA 311

Query: 338 KNHQAVALRSGSDLSVFYRCGFEGFQDTLYVYAERQFYKQCDIYGTVDFIFGNAAVVIQD 397
            N QAVALRSGSDLSVFY+C FE +QDTLYV++ RQFY+ CD+YGTVDFIFGNAA V+Q+
Sbjct: 312 SNEQAVALRSGSDLSVFYQCSFEAYQDTLYVHSNRQFYRDCDVYGTVDFIFGNAAAVLQN 371

Query: 398 SNIIARDPPNKTITLTAQGRSDPNQNTGISIHNCRVTSAGGLSGV----KAYLGRPWRQY 457
            NI AR P +KT T+TAQGRSDPNQNTGI IHN RVT+A  L  V    K YLGRPWRQY
Sbjct: 372 CNIFARRPRSKTNTITAQGRSDPNQNTGIIIHNSRVTAASDLRPVLGSTKTYLGRPWRQY 431

Query: 458 SRTVFMKSNIGGFINPAGWMEWSGNFALNTLYYGEYSNTGPGSSTANRVKWKGYHVITSA 517
           SRTVFMK+++   I+P GW+EW GNFAL TL+Y E+ NTGPG+ST+ RV W G+ V+ SA
Sbjct: 432 SRTVFMKTSLDSLIDPRGWLEWDGNFALKTLFYAEFQNTGPGASTSGRVTWPGFRVLGSA 491

Query: 518 AEASKFTVGNFIAGGSWLPNTGVPFTSGL 540
           +EASKFTVG F+AGGSW+P++ VPFTSGL
Sbjct: 492 SEASKFTVGTFLAGGSWIPSS-VPFTSGL 511

BLAST of Moc05g02900 vs. TAIR 10
Match: AT3G47400.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 454.5 bits (1168), Expect = 1.2e-127
Identity = 253/508 (49.80%), Postives = 331/508 (65.16%), Query Frame = 0

Query: 59  WCSQTPYPAPCEHFLRTKAATS-NNSPITDKSHFLKILVETALSTAVSAHKDAISLGPKC 118
           WC++TP+   C ++ R  +  + N  P   +S FL++LV+ AL  AV  H   +  GP C
Sbjct: 87  WCNKTPHAETCNYYFRKSSQNNINLRPPRFRSEFLRMLVKVALDQAVITHSQTVKFGPSC 146

Query: 119 RNSREKSAWADCVYLYDQIISRLNRTAD----------RCSRSDAQTWLSAALTALETCR 178
            N++ K+AW+DCV L+   +++LNRT            +C+  DAQTWLS A T +ETCR
Sbjct: 147 TNNQRKAAWSDCVNLFQNTVAQLNRTLKGLNPAASSDVKCTDFDAQTWLSTAQTNIETCR 206

Query: 179 TGFDELGVSASGYPLTAN-NVSKLISDGLSVNG--PPSPDGFGPPSRVDGFPVWVSPGDR 238
           +G ++L VS    P+ +N N+S LI + L+VNG      D     +  + FP WVS  +R
Sbjct: 207 SGSEDLNVSDFVMPVISNKNLSDLIGNCLAVNGVLMKQHDHTTTANHKEYFPSWVSRHER 266

Query: 239 KLLQSGSPA-GNADVVVAKDGSGDFQTVAAAV---AAAKGGGRFVIYVKAGVYSENVEI- 298
           +LL S S A  +  +VVA+D SG F+++ AA+   A  +   RFVIYVK GVY EN+++ 
Sbjct: 267 RLLVSASLAKSSPHLVVAQDRSGHFRSIQAAINFAARRRFKSRFVIYVKKGVYRENIDVG 326

Query: 299 -KAKNVMLVGDGIGKTVITGSRSVGGGSTTFRSATVAVDGDGFIARDITFRNTAGAKNHQ 358
               N+MLVGDG  KT+IT  RSV  G TT+ SAT    G  F+A+D+TF NTAG    Q
Sbjct: 327 NDNHNIMLVGDGERKTIITSGRSVQHGYTTYNSATGGFGGQRFVAKDMTFINTAGPLRGQ 386

Query: 359 AVALRSGSDLSVFYRCGFEGFQDTLYVYAERQFYKQCDIYGTVDFIFGNAAVVIQDSNII 418
           AVA+RS SDLSVFYR G  GFQDTLY++++RQF+++C I GT+DFIFGNAAVV Q+  I+
Sbjct: 387 AVAVRSSSDLSVFYRVGIHGFQDTLYIHSQRQFFRECYISGTIDFIFGNAAVVFQNCMIL 446

Query: 419 ARDP-PNKTITLTAQGRSDPNQNTGISIHNCRVTSAGGLSGV----KAYLGRPWRQYSRT 478
            R P   +   +TAQGR DP QNTGI+IH+ R+ +A  L  V    K YLGRPW+ YSR 
Sbjct: 447 VRRPLHGQANIITAQGRGDPFQNTGITIHSSRIIAASDLKPVIRAYKTYLGRPWQAYSRV 506

Query: 479 VFMKSNIGGFINPAGWMEW--SGNFALNTLYYGEYSNTGPGSSTANRVKWKGYHVITSAA 538
             MK+ I   I+P GW  W    NFALNT++YGEY N GPGSST  RV+WKG+H ITS A
Sbjct: 507 TIMKTYIDNSISPLGWSPWLRGSNFALNTVFYGEYKNFGPGSSTRWRVRWKGFHAITSTA 566

Query: 539 EASKFTVGNFIAGGSWLPNTGVPFTSGL 540
            AS+FTVG+ IAGGSWLP TGVPF SGL
Sbjct: 567 VASRFTVGSLIAGGSWLPATGVPFKSGL 594

BLAST of Moc05g02900 vs. TAIR 10
Match: AT5G51490.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 454.5 bits (1168), Expect = 1.2e-127
Identity = 256/533 (48.03%), Postives = 339/533 (63.60%), Query Frame = 0

Query: 33  LSVVALVVVLVFPSAIFGYSEKEIKSWCSQTPYPAPCEHFLRTKAATSNNSPITDKSHFL 92
           L ++ LV++ V P  +   +++    WC +TPYP PC+ + +     +     T  S F 
Sbjct: 13  LHLILLVLLCVHP--LTTVADRNSTDWCDKTPYPDPCKCYFKNH---NGFQQPTQLSEFR 72

Query: 93  KILVETALSTAVSAHKDAISLGPKCRNSREKSAWADCVYLYDQIISRLNRT--------- 152
            +LVE A+  A+SA  +  + G  C +S++++  ADC+ LY   I +LNRT         
Sbjct: 73  VMLVEAAMDRAISARAELTNSGKNCTDSKKQAVLADCIDLYGDTIMQLNRTLHGVSPKAG 132

Query: 153 -ADRCSRSDAQTWLSAALTALETCRTGFDELGVSASGYPLTAN-NVSKLISDGLSVNGPP 212
            A  C+  DAQTWLS ALT  ETCR G  +L V+    P+ +N  +S LIS+ L+VNG  
Sbjct: 133 AAKSCTDFDAQTWLSTALTNTETCRRGSSDLNVTDFITPIVSNTKISHLISNCLAVNGAL 192

Query: 213 SPDG--FGPPSRVDGFPVWVSPGDRKLLQSGSPAGNADVVVAKDGSGDFQTVAAAVAAA- 272
              G      +   GFP W+S  D++LL+    A  A++VVAKDGSG F TV AA+  A 
Sbjct: 193 LTAGNKGNTTANQKGFPTWLSRKDKRLLR----AVRANLVVAKDGSGHFNTVQAAIDVAG 252

Query: 273 ---KGGGRFVIYVKAGVYSENVEIKAKN--VMLVGDGIGKTVITGSRSVGGGSTTFRSAT 332
                 GRFVIYVK G+Y EN+ ++  N  +MLVGDG+  T+ITG RSV GG TT+ SAT
Sbjct: 253 RRKVTSGRFVIYVKRGIYQENINVRLNNDDIMLVGDGMRSTIITGGRSVQGGYTTYNSAT 312

Query: 333 VAVDGDGFIARDITFRNTAGAKNHQAVALRSGSDLSVFYRCGFEGFQDTLYVYAERQFYK 392
             ++G  FIA+ ITFRNTAG    QAVALRS SDLS+FY+C  EG+QDTL V+++RQFY+
Sbjct: 313 AGIEGLHFIAKGITFRNTAGPAKGQAVALRSSSDLSIFYKCSIEGYQDTLMVHSQRQFYR 372

Query: 393 QCDIYGTVDFIFGNAAVVIQDSNIIARDP-PNKTITLTAQGRSDPNQNTGISIHNCRVTS 452
           +C IYGTVDFIFGNAA V Q+  I+ R P   +   +TAQGR+DP QNTGISIHN R+  
Sbjct: 373 ECYIYGTVDFIFGNAAAVFQNCLILPRRPLKGQANVITAQGRADPFQNTGISIHNSRILP 432

Query: 453 AGGL----SGVKAYLGRPWRQYSRTVFMKSNIGGFINPAGWMEW--SGNFALNTLYYGEY 512
           A  L      VK Y+GRPW ++SRTV +++ +   ++P GW  W     F L+TL+Y EY
Sbjct: 433 APDLKPVVGTVKTYMGRPWMKFSRTVVLQTYLDNVVSPVGWSPWIEGSVFGLDTLFYAEY 492

Query: 513 SNTGPGSSTANRVKWKGYHVITSAAEASKFTVGNFIAGGSWLPNTGVPFTSGL 540
            NTGP SST  RV WKG+HV+  A++AS FTVG FIAG +WLP TG+PFTSGL
Sbjct: 493 KNTGPASSTRWRVSWKGFHVLGRASDASAFTVGKFIAGTAWLPRTGIPFTSGL 536

BLAST of Moc05g02900 vs. TAIR 10
Match: AT4G00190.1 (pectin methylesterase 38 )

HSP 1 Score: 442.6 bits (1137), Expect = 4.6e-124
Identity = 247/494 (50.00%), Postives = 314/494 (63.56%), Query Frame = 0

Query: 60  CSQTPYPAPCEHFLRTKAATSNNSPITDKSHFLKILVETALSTAVSAHKDAISLGPKCRN 119
           C +TP+P  C      K     + PI   + FL++ VE  L  AV A  D   L P+  +
Sbjct: 8   CDETPHPGEC------KTLLIKHKPIRSTTQFLQVSVERTLDGAVKAKSDTYFLEPQFGS 67

Query: 120 SREKSAWADCVYLYDQIISRLNRTA----DRCSRSDAQTWLSAALTALETCRTGFDELGV 179
              K AW +C+ LY+Q I RLN +     + CSRSD Q WLS ALT L+TC+    ELGV
Sbjct: 68  ---KQAWEECMDLYEQTIHRLNESVLCPKNVCSRSDVQAWLSTALTNLDTCQEEMSELGV 127

Query: 180 SASGYPLTANNVSKLISDGLSVNGPPSPDG--FGPPSRVDGFPVWVSPGDRKLLQSGSPA 239
           S+     +  +++  + + L++N     +G  FG           +S    K L  G   
Sbjct: 128 SSH----SLESITIDVINTLAINKRMEQNGKEFG-----------ISKITMKTLSIGE-- 187

Query: 240 GNADVVVAKDGSGDFQTVAAAVAAA----KGGGRFVIYVKAGVYSE--NVEIKAKNVMLV 299
              DVVVA+DGSGD++T+  AV  A    KG  R+VI+VK GVY E  NV IK+ N+M+ 
Sbjct: 188 -KVDVVVAQDGSGDYKTIQEAVNGAGERLKGSPRYVIHVKQGVYEEYVNVGIKSNNIMIT 247

Query: 300 GDGIGKTVITGSRSVGGGSTTFRSATVAVDGDGFIARDITFRNTAGAKNHQAVALRSGSD 359
           GDGIGKT+ITG +S G G +T++SAT   +GDGF+ RDIT RNTAG +NHQAVALRS SD
Sbjct: 248 GDGIGKTIITGDKSKGRGFSTYKSATFVAEGDGFVGRDITIRNTAGPENHQAVALRSNSD 307

Query: 360 LSVFYRCGFEGFQDTLYVYAERQFYKQCDIYGTVDFIFGNAAVVIQDSNIIARDPPNKTI 419
           +SVFYRC  EG+QDTLYV++ RQF+++CDIYGTVDFIFGNAA V+Q+  I AR+PPN   
Sbjct: 308 MSVFYRCSIEGYQDTLYVHSGRQFFRECDIYGTVDFIFGNAAAVLQNCRIFARNPPNGVN 367

Query: 420 TLTAQGRSDPNQNTGISIHNCRVTSAGG--LSGVKAYLGRPWRQYSRTVFMKSNIGGFIN 479
           T+TAQ R +PNQ TGI IHN  V  A G  L GVK YLGRPWR Y+RTV + + +   I 
Sbjct: 368 TITAQSRFNPNQTTGIVIHNSVVKGAPGVQLGGVKTYLGRPWRSYARTVVIGTYLDTLIE 427

Query: 480 PAGWMEWSGNFALNTLYYGEYSNTGPGSSTANRVKWKGYHVITSAAEASKFTVGNFIAGG 539
           P GW++W    AL+TLYYGEY N+GPGS T NRV W G+HVI+   EA +FT+  FI   
Sbjct: 428 PNGWIDWDNVTALSTLYYGEYQNSGPGSGTENRVDWAGFHVISDIQEAREFTLPKFIDSA 474

BLAST of Moc05g02900 vs. TAIR 10
Match: AT5G51500.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 441.8 bits (1135), Expect = 7.8e-124
Identity = 254/533 (47.65%), Postives = 336/533 (63.04%), Query Frame = 0

Query: 32  SLSVVALVVVLVFP-SAIFGYSEKEIKSWCSQTPYPAPCEHFLRTKAATSNNSPITDKSH 91
           SL ++ ++++ + P + +   +   I  WC +TPYP PC+ +       S     T  S 
Sbjct: 13  SLHLLLILLLCLRPLTTVADGNSTNIDGWCDKTPYPYPCKRYF---IKHSGFRLPTQISE 72

Query: 92  FLKILVETALSTAVSAHKDAISLGPKCRNSREKSAWADCVYLYDQIISRLNRTAD----- 151
           F  +LVE A+  AVSA     +    C + ++++  ADC+ LY   + +LNRT       
Sbjct: 73  FRVLLVEAAMDRAVSAWDKLTNSSKNCTDFKKQAVLADCINLYGDTVMQLNRTLQGVSSK 132

Query: 152 ---RCSRSDAQTWLSAALTALETCRTGFDELGVSASGYPLTAN-NVSKLISDGLSVNGPP 211
              RC+  DAQTWLS ALT  ETCR G  +L VS    P+ +N  +S LIS+ L+VNG  
Sbjct: 133 TGRRCTDFDAQTWLSTALTNTETCRRGSSDLNVSDFTTPIVSNTKISHLISNCLAVNGAL 192

Query: 212 SPDGFGPPSRVD--GFPVWVSPGDRKLLQSGSPAGNADVVVAKDGSGDFQTVAAAVAAA- 271
              G    +  D  GFP WVS  +R+LLQ  S    A++VVAKDGSG F+TV AA+  A 
Sbjct: 193 LTAGKNDSTTGDSKGFPTWVSRKERRLLQLQSV--RANLVVAKDGSGHFKTVQAAIDVAG 252

Query: 272 ---KGGGRFVIYVKAGVYSENVEIKAK--NVMLVGDGIGKTVITGSRSVGGGSTTFRSAT 331
                 GRFVIYVK G+Y EN+ ++    N+MLVGDG+  T+ITG RSV GG TT+ SAT
Sbjct: 253 RRKVTSGRFVIYVKRGIYQENLNVRLNNDNIMLVGDGMRYTIITGGRSVKGGYTTYSSAT 312

Query: 332 VAVDGDGFIARDITFRNTAGAKNHQAVALRSGSDLSVFYRCGFEGFQDTLYVYAERQFYK 391
             ++G  FIA+ I F+NTAG    QAVALRS SDLS+FYRC  EG+QDTL V+++RQFY+
Sbjct: 313 AGIEGLHFIAKGIAFQNTAGPAKGQAVALRSSSDLSIFYRCSIEGYQDTLMVHSQRQFYR 372

Query: 392 QCDIYGTVDFIFGNAAVVIQDSNIIARDP-PNKTITLTAQGRSDPNQNTGISIHNCRVTS 451
           +C IYGTVDFIFGNAAVV Q+  I+ R P   +   +TAQGR+D  QNTGISIHN  +  
Sbjct: 373 ECYIYGTVDFIFGNAAVVFQNCIILPRLPLKGQANVITAQGRTDLFQNTGISIHNSIIIP 432

Query: 452 AGGLS----GVKAYLGRPWRQYSRTVFMKSNIGGFINPAGWMEWS--GNFALNTLYYGEY 511
           A  L      VK Y+GRPW  YSRTV +K+ I   ++P GW  W+    + L+TL+Y EY
Sbjct: 433 APDLKPVVRSVKTYMGRPWMMYSRTVVLKTYIDSVVSPVGWSPWTKGSTYGLDTLFYAEY 492

Query: 512 SNTGPGSSTANRVKWKGYHVITSAAEASKFTVGNFIAGGSWLPNTGVPFTSGL 540
            N GP SST  RV+WKG+HV++ A++AS F+VG FIAG +WLP +G+PFTS L
Sbjct: 493 KNIGPASSTRWRVRWKGFHVLSKASDASAFSVGKFIAGTAWLPGSGIPFTSEL 540