Moc05g02750 (gene) Bitter gourd (OHB3-1) v2

Overview
NameMoc05g02750
Typegene
OrganismMomordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
Description3'-5' ssDNA/RNA exonuclease TatD
Locationchr5: 1881883 .. 1887359 (+)
RNA-Seq ExpressionMoc05g02750
SyntenyMoc05g02750
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAATTGGATATTTTCTTTGACGTATTGTTCTGATCGTTCGAAATTGATCGCGTTTCCGGTTTGCTCTAATAATTCGCTTCATTCTTCGGATTCTCGGAATTGGAAGACGAAGGTACTGTGCTATCTATGTGATTTTCCGTATCTCTCTCGTCTTGCTGATTCTCCAATTTCTGAAATCTTCAGTAAATGTAGTCGTAGGTCTTGTCTTGGCGGGAAATGTTCTTACGCTCTTTCTCGTCATGATTAAGTACATTGTGAAGAGTTGGACTGAAAATGGATGTAAATTGTAATAGGATTTTACGTTGAGATCCTTCACGATTGGAAAACTTAGGCACTGTGGTAGCTGTGCTATTTTCCGTATCTTTATCTCGTTCGCTCGTTCTCCAATTTCTTAGATCTTTTGCAGTATATGAAATCGTAGGTTTCTCTGAGCCGGAAATGTTCTTACACACTTTCTCGCACATAATTTATTGGATGTAGTAAGCTCTTTTCGCCTTTAAGTTGAAATCCTTCTCAATTGGAAAGCGAAGGTACTGTGCTAGCTGTGTGATTTTCCGTATATCTCTCTCACTCGCTGATTCTCCAATTTCTTAAATTTTCTGCAGTAACTGAACTAGTAGGTTTTCTCTGAGCAGTGTTCTAACGCACTTTCTCAAGATGATGAATTACATAATCAAGGATTGAATTTAAATGGATATAATAGGCTTTTAAGTTGAAATTCTTCACAGTTGGAAATTGCAGGTACTGTACTAGCTGTGCTATTTTCCTATATTCTAGGGTCTCTGATTCTCCAATTTCTTAAATCTTCTATAATAAATGAACTTGTAGGTTTCTATGAATCGGAAATGTTCTTACACACTTTCTTGACAAGATTAATTACATTTTGAAGAACTTATATGGATATAATAGGCTTTGAAGTTTAAATCCTTAAACCAACATAAGAAGAAACTCTTTGTCTTGGATCTGTTTTGAGTCTTTAGAGGGAAAAAATATCTCATCTCGATTGCTCTATTTCTGTCGGTTTTTGTCATTTGGCCTCTCATTTGTATCTGTAGTATATACATCTTTTGTATCAGATTGTGTGTATATTGGCATTCGAGACTGTATAAGTCATTCAATTGAGACCATTGGACAATTGATGTTTCCTTCTGGGTCTCGGAAATTTTTCTACATTTCTTTCTTTCAGCAACTTTCCTCGTTTTACAGACACGGGGATGGCAAAATTATCTGCTACATTGAGACTATTTGATGCACATTGTCACCTCCAAGATCCAAGAATATTTGACAAAGCACCTCAATTAATTAGCACAGCAGTGGACTCTGGTGTTGTTCGTTTTGCTGTTAATGGAATCTCTGAGGTACGATAGTTTTGCTTTGCATTCTTTGTTGTATATGTTGTTTTCAGATTTGTGCTGCCTGCCACTCATCTCTTTCCATTGTTTTCATGTTCATAGAAAGACTGGCATTTGGTCAAGCAACTGAGTGAATTACATCCTTGCATAGTTCCATCGTTCGGTCTTCATCCTTGGTAACCACTGTCCATCTCCAATACGCAGAAATCATGTATTCCATCTTCTTTTACCGGCATGCCACATATTTGCGAGGTATATGGTTGATGACATTCATCCATTTTGTTGTATTTAGGTATGTTCCTGAAAGAACTCCCGGCTGGCTTGAAAAACTTAAGGAATTTTTTGAAGCAGTGCCAAACGCAGCGGTTGGAGAGGTTGGTACTTGTGGCAGTGGCCAAAGCTATTTCCTTGACCAATTTGAGCTTTGGATATTTTATTAGGAAGCAATTTCATTTTTAAATGAAATAGGGTGAAAAGATTTAAAAATTAAAAAAGTGAGTCAGATGAGTTCTTTTTGCTTGATAATTATTCTATTCTTCTAGCATTATCCCTGGAACCAGCTTAAGAGGCATGTTTTGGGTGATTTCTGAAGTTATTGATCCATGATTGTTGACTGTTTTTCTGGAGAAAGACAGTCAATGTTCAGGTGTATGACAGATACTTATACTGAACCTTTCATGCTTATACATGGTACTAGTGCCTTCCTAAATTATGTGCCCTAAACTTTTATATTCCGTCATCTTTTATCTACCTGCTAGGATCCAGGGTAGCACATAGGTCTTTAGTGATTAATTTGTTGCCTCAACTAGCAGAGGTATTCTTTTAGTAGGTTTCCAAATTCTGTAGGGCTCTAATTTTTACCTTTTCAGATTGGTCTGGACAAAGGTTCACGAGGAAGGGAGATCAGTTTTACAGATCAGGTGTGACTATTCTACATTTTCTTCTGAGCTGATGATAGAGGTCGATGATCATTGTCCACAATGGTTTCTTGTTTCCTGTCAAAAAGGAAATTTTAAATGGTTTAAGAAGCAAATTTTCTAAACAACTGCAGTGATTTTCGTTTTTTTTTTTATGATTCCCAGACTGTTCTTGACATAAAAAGATGCTGCAGGTTGAGGTCTTTCAGCAACAACTTGAACTTGCTAAGTCTTTAGAAAAACCAGCATCCATCCATTGTGTTCGTGCTTTTGGTGATCTCCTTCAGATAATGAAGTATGACTCTTTCTAAGAATTTATGGGTTTTCTTGTTATCTGTGCTGTTCACATTCTCAATGGGGTGTCAAAATATATCTTGAATTGATATTTCTTCATCCGGCTGGGTATATTTTATAATTTTTTATTTTCTTCTTTATAACTAATATGTTTGCGGAACACTGAACCTGGGGAACCATTGTTTAGCATGGGCGCTAGTCTTAGAATTTTCATGGTTTAATTCTCCTCTTTAGCTCTAAGCTCTAGAGACACATAACTCTCCAAGAATGTCTATTAGTCTTTTTGGCTGTTGTTTGTTTGGGGTAGGGGTGGTATTTGTTTATGTCAAAAGTTGTCACTTTTAGTCATGTCCCACATTTGTTTATGTGTTGACATGTCTTGTTCCGTGTCATCACAATTGCCTCAGATCTATGGGACCTTTTCCTGCTGGTTTCATTCTGCATTCTTATCTAGGATCTGCTCAGATGGTTCCTGAGTTCTCCAAGCTAGGTGGTTACTTTTCATTTTCCGGTTTTCTCATGTCCATGGATGCAAAGAAGGCAAAGAAAATGCTGAAAATGGTTGGTTTGCTTTTGTCATGCATATTGTAATTTATGGTTTTTTTTTCTTTTGGAGTTTCTGTTGTGTGGGTAAAAGATACCAGATTAGATATTTGTTAAATCACCACTAAATTCAAAAATTTAAACCGATATATTATGGAAAATATTAATCACTCTCTTTCCTCGTGACCTCGGAAATTTTCACAATGCTCAATGAGTGGTTATCAATATAAATTGGGGAGGAAATAAAATTTTAGGGCTTTAAACATATGTCTATCATGTTAAATCCCAAAAGCTTAAGCCGATGGATTATATATAATCATTTATCAATACTTTAACAATATTAACCATACGTATATGAAAGAAAATATACGAGTACAAAATAGGGTGGAAGTAAATATTTCCTAAAAAGGCAGTGGATCATAAAAATCACATCCTATTGGCTTGTATGACAGAAATAATAATTACAAAAACTTCTGTCTAGGGCGCACCATCTGAAAGAATGGAGCCCACCAAATCCTATATGTGTCTAAACATCGTGAATTTTTCCAGGTGCCCTCGGATAGAATTTTGTTGGAGACTGATGCACCTGATGCACTTCCCAAGTTAAAGTTAGACTCTATCTATTTTGTTGAAGGCGATCCTTCCCTTCCTGAAGGTCTCAAGACCGGAGAACAATCTGCCTCAACAGGCTCAGAACAAAGTACTTTGCCTTCTTCTACAGATGCATCAACACTGCCAAAAGAGACTCTCAATCATCCAGCAAACATCCAAAATGTACTTCAGTAAATCTCTTATTCATATCTGGAAGATGGATATTCATATTCATGCATATGTATAAAAAGTGAGGAGCATATATTGTTGGTCTAACAACTTGGCATGGCACGTGGCACCCATGGTTCATTATAACAAGCTTAAAGAAAACCTGCATAACAGCTCTCTGATGATCATTGATAGCATTTTATGATAAAGATCAGCTTTTAATACTAGTTAAACCTATATATAAAGTTTCTTTTACTCTTCAATATGAGGGCATAGAGAAGTAGAATCAGCCTCGGCTTCCGTCATCCTTGTTTAATTATTACATGCTGGCGTTCTTTAGAATCTTAAATTAAAAGTTTATATAGCTCTAGATAGTAGATTAGTTCATTTTTCATTATCAATTATGTTTTTATAAAATATATTTTATGCTTAATTCTTCTCTACAGGTCCTAGTTTATGTGGCATCTTTGTTGGAAATCAGTAAAGAAGAACTTGCTGAATTGAGTTACAGAAATGCGGAACATTTATTCTCTTTCAAAGGTCTACAAACTTCTTAGTGAACAGTCCGAATATTTAGGTGATGTTTATAATAACTCGATATAATTCCCTCTATTTTTCTCAATTCTCTGACATGTATCTTACCATATAGCAATGAACTCTTCTTGTGTGGAATGAAAGTAATAGGTGATACCTTTACAGTACTTGTATTCGGTATTGTGTACCAAGAACATCTAGAACCATCAAACGATGGGCAATGCTGGACACATGACATTTCAGAGGTAGGTATATGTGTTAACTAGGTCGTTAGACCACTTACCTAGTGTCCATGCATTCAAATTATTTTCGAGTATATGAATGTGATGGAGATGACTATTAGTAGGGACATTGCATTAAAGCTGCTAGAACCAGTTCAATCTTGGAACTGTAAGGTCATAAGATGAACAAAGATATGAGCAGAAGCCAGGTAATTTATCTGCTGAAACTCTTCCTCTCTCTCATCCTTAGATAAACTTGGTCTAGCTTCTCTGTCATAATTGGACTCTCACAGATTAAAGAAGTATGAAAAATTGATTAGTAGGGGGCATAAAGTTTTTAAATATATGTAATATTAGCTCGAGTCAATGCATGCATACAGAATATGCTTGTGACACAATCGGACAAAAAATTTATCTCTAGTCTATATGCCTGTATAACTTTTCAGCACAAAAACATATTTCTGCCTTTAAACTTGGAATGAAGAAATTTTCTTCAACTATTTGTTCTATCCAAATGGCGAATCCATTCCTATGCCCAGCAAATTCATTTTGATCATTGTCTGTTAACGTTTGGTTGGTGCTATTGCTGTTTATATTTAGTTGCTTATTCCGTAGGTGTTCCATTACTTTTACTTTTCAGTAATATAGTTTTAATAATGTAGAGCTTTTCTACAGTTATGGTGTAGTGTTGGAAAAATCTTTTCAATTTAGATTTTTATTTATTTTTTAACAGAATACAGTAGAGTTAAACCTTCAGAAAATACCAAAACTAGGCACTTGTCTGTGATGAGCGTGCGCATAATTGTATCCAGTCTGATGAAGATGAGACTTCTCGAAATTAAGGGAGATCAGTAA

mRNA sequence

ATGAATTGGATATTTTCTTTGACGTATTGTTCTGATCGTTCGAAATTGATCGCGTTTCCGGTTTGCTCTAATAATTCGCTTCATTCTTCGGATTCTCGGAATTGGAAGACGAAGGTACTGTGCTATCTATGTGATTTTCCGTATCTCTCTCGTCTTGCTGATTCTCCAATTTCTGAAATCTTCAGTAAATGTAGTCGTAGCAACTTTCCTCGTTTTACAGACACGGGGATGGCAAAATTATCTGCTACATTGAGACTATTTGATGCACATTGTCACCTCCAAGATCCAAGAATATTTGACAAAGCACCTCAATTAATTAGCACAGCAGTGGACTCTGGTGTTGTTCGTTTTGCTGTTAATGGAATCTCTGAGAAAGACTGGCATTTGGTCAAGCAACTGAGTGAATTACATCCTTGCATAGTTCCATCGTTCGGTCTTCATCCTTGGTATGTTCCTGAAAGAACTCCCGGCTGGCTTGAAAAACTTAAGGAATTTTTTGAAGCAGTGCCAAACGCAGCGGTTGGAGAGATTGGTCTGGACAAAGGTTCACGAGGAAGGGAGATCAGTTTTACAGATCAGGTTGAGGTCTTTCAGCAACAACTTGAACTTGCTAAGTCTTTAGAAAAACCAGCATCCATCCATTGTGTTCGTGCTTTTGGTGATCTCCTTCAGATAATGAAATCTATGGGACCTTTTCCTGCTGGTTTCATTCTGCATTCTTATCTAGGATCTGCTCAGATGGTTCCTGAGTTCTCCAAGCTAGGTGGTTACTTTTCATTTTCCGGTTTTCTCATGTCCATGGATGCAAAGAAGGCAAAGAAAATGCTGAAAATGGTGCCCTCGGATAGAATTTTGTTGGAGACTGATGCACCTGATGCACTTCCCAAGTTAAAGTTAGACTCTATCTATTTTGTTGAAGGCGATCCTTCCCTTCCTGAAGGTCTCAAGACCGGAGAACAATCTGCCTCAACAGGCTCAGAACAAAGTACTTTGCCTTCTTCTACAGATGCATCAACACTGCCAAAAGAGACTCTCAATCATCCAGCAAACATCCAAAATGTCCTAGTTTATGTGGCATCTTTGTTGGAAATCAGTAAAGAAGAACTTGCTGAATTGAGTTACAGAAATGCGGAACATTTATTCTCTTTCAAAGGTGATACCTTTACAGTACTTGTATTCGGTATTGTGTACCAAGAACATCTAGAACCATCAAACGATGGGCAATGCTGGACACATGACATTTCAGAGGTAGAATACAGTAGAGTTAAACCTTCAGAAAATACCAAAACTAGGCACTTGTCTGTGATGAGCGTGCGCATAATTGTATCCAGTCTGATGAAGATGAGACTTCTCGAAATTAAGGGAGATCAGTAA

Coding sequence (CDS)

ATGAATTGGATATTTTCTTTGACGTATTGTTCTGATCGTTCGAAATTGATCGCGTTTCCGGTTTGCTCTAATAATTCGCTTCATTCTTCGGATTCTCGGAATTGGAAGACGAAGGTACTGTGCTATCTATGTGATTTTCCGTATCTCTCTCGTCTTGCTGATTCTCCAATTTCTGAAATCTTCAGTAAATGTAGTCGTAGCAACTTTCCTCGTTTTACAGACACGGGGATGGCAAAATTATCTGCTACATTGAGACTATTTGATGCACATTGTCACCTCCAAGATCCAAGAATATTTGACAAAGCACCTCAATTAATTAGCACAGCAGTGGACTCTGGTGTTGTTCGTTTTGCTGTTAATGGAATCTCTGAGAAAGACTGGCATTTGGTCAAGCAACTGAGTGAATTACATCCTTGCATAGTTCCATCGTTCGGTCTTCATCCTTGGTATGTTCCTGAAAGAACTCCCGGCTGGCTTGAAAAACTTAAGGAATTTTTTGAAGCAGTGCCAAACGCAGCGGTTGGAGAGATTGGTCTGGACAAAGGTTCACGAGGAAGGGAGATCAGTTTTACAGATCAGGTTGAGGTCTTTCAGCAACAACTTGAACTTGCTAAGTCTTTAGAAAAACCAGCATCCATCCATTGTGTTCGTGCTTTTGGTGATCTCCTTCAGATAATGAAATCTATGGGACCTTTTCCTGCTGGTTTCATTCTGCATTCTTATCTAGGATCTGCTCAGATGGTTCCTGAGTTCTCCAAGCTAGGTGGTTACTTTTCATTTTCCGGTTTTCTCATGTCCATGGATGCAAAGAAGGCAAAGAAAATGCTGAAAATGGTGCCCTCGGATAGAATTTTGTTGGAGACTGATGCACCTGATGCACTTCCCAAGTTAAAGTTAGACTCTATCTATTTTGTTGAAGGCGATCCTTCCCTTCCTGAAGGTCTCAAGACCGGAGAACAATCTGCCTCAACAGGCTCAGAACAAAGTACTTTGCCTTCTTCTACAGATGCATCAACACTGCCAAAAGAGACTCTCAATCATCCAGCAAACATCCAAAATGTCCTAGTTTATGTGGCATCTTTGTTGGAAATCAGTAAAGAAGAACTTGCTGAATTGAGTTACAGAAATGCGGAACATTTATTCTCTTTCAAAGGTGATACCTTTACAGTACTTGTATTCGGTATTGTGTACCAAGAACATCTAGAACCATCAAACGATGGGCAATGCTGGACACATGACATTTCAGAGGTAGAATACAGTAGAGTTAAACCTTCAGAAAATACCAAAACTAGGCACTTGTCTGTGATGAGCGTGCGCATAATTGTATCCAGTCTGATGAAGATGAGACTTCTCGAAATTAAGGGAGATCAGTAA

Protein sequence

MNWIFSLTYCSDRSKLIAFPVCSNNSLHSSDSRNWKTKVLCYLCDFPYLSRLADSPISEIFSKCSRSNFPRFTDTGMAKLSATLRLFDAHCHLQDPRIFDKAPQLISTAVDSGVVRFAVNGISEKDWHLVKQLSELHPCIVPSFGLHPWYVPERTPGWLEKLKEFFEAVPNAAVGEIGLDKGSRGREISFTDQVEVFQQQLELAKSLEKPASIHCVRAFGDLLQIMKSMGPFPAGFILHSYLGSAQMVPEFSKLGGYFSFSGFLMSMDAKKAKKMLKMVPSDRILLETDAPDALPKLKLDSIYFVEGDPSLPEGLKTGEQSASTGSEQSTLPSSTDASTLPKETLNHPANIQNVLVYVASLLEISKEELAELSYRNAEHLFSFKGDTFTVLVFGIVYQEHLEPSNDGQCWTHDISEVEYSRVKPSENTKTRHLSVMSVRIIVSSLMKMRLLEIKGDQ
Homology
BLAST of Moc05g02750 vs. NCBI nr
Match: XP_022146895.1 (uncharacterized protein LOC111015984 [Momordica charantia] >XP_022146896.1 uncharacterized protein LOC111015984 [Momordica charantia] >XP_022146897.1 uncharacterized protein LOC111015984 [Momordica charantia])

HSP 1 Score: 617.5 bits (1591), Expect = 9.5e-173
Identity = 309/309 (100.00%), Postives = 309/309 (100.00%), Query Frame = 0

Query: 77  MAKLSATLRLFDAHCHLQDPRIFDKAPQLISTAVDSGVVRFAVNGISEKDWHLVKQLSEL 136
           MAKLSATLRLFDAHCHLQDPRIFDKAPQLISTAVDSGVVRFAVNGISEKDWHLVKQLSEL
Sbjct: 1   MAKLSATLRLFDAHCHLQDPRIFDKAPQLISTAVDSGVVRFAVNGISEKDWHLVKQLSEL 60

Query: 137 HPCIVPSFGLHPWYVPERTPGWLEKLKEFFEAVPNAAVGEIGLDKGSRGREISFTDQVEV 196
           HPCIVPSFGLHPWYVPERTPGWLEKLKEFFEAVPNAAVGEIGLDKGSRGREISFTDQVEV
Sbjct: 61  HPCIVPSFGLHPWYVPERTPGWLEKLKEFFEAVPNAAVGEIGLDKGSRGREISFTDQVEV 120

Query: 197 FQQQLELAKSLEKPASIHCVRAFGDLLQIMKSMGPFPAGFILHSYLGSAQMVPEFSKLGG 256
           FQQQLELAKSLEKPASIHCVRAFGDLLQIMKSMGPFPAGFILHSYLGSAQMVPEFSKLGG
Sbjct: 121 FQQQLELAKSLEKPASIHCVRAFGDLLQIMKSMGPFPAGFILHSYLGSAQMVPEFSKLGG 180

Query: 257 YFSFSGFLMSMDAKKAKKMLKMVPSDRILLETDAPDALPKLKLDSIYFVEGDPSLPEGLK 316
           YFSFSGFLMSMDAKKAKKMLKMVPSDRILLETDAPDALPKLKLDSIYFVEGDPSLPEGLK
Sbjct: 181 YFSFSGFLMSMDAKKAKKMLKMVPSDRILLETDAPDALPKLKLDSIYFVEGDPSLPEGLK 240

Query: 317 TGEQSASTGSEQSTLPSSTDASTLPKETLNHPANIQNVLVYVASLLEISKEELAELSYRN 376
           TGEQSASTGSEQSTLPSSTDASTLPKETLNHPANIQNVLVYVASLLEISKEELAELSYRN
Sbjct: 241 TGEQSASTGSEQSTLPSSTDASTLPKETLNHPANIQNVLVYVASLLEISKEELAELSYRN 300

Query: 377 AEHLFSFKG 386
           AEHLFSFKG
Sbjct: 301 AEHLFSFKG 309

BLAST of Moc05g02750 vs. NCBI nr
Match: KAA0049069.1 (putative metal-dependent hydrolase YabD isoform X1 [Cucumis melo var. makuwa] >TYK17493.1 putative metal-dependent hydrolase YabD isoform X1 [Cucumis melo var. makuwa])

HSP 1 Score: 577.4 bits (1487), Expect = 1.1e-160
Identity = 292/331 (88.22%), Postives = 307/331 (92.75%), Query Frame = 0

Query: 77  MAKLSATLRLFDAHCHLQDPRIFDKAPQLISTAVDSGVVRFAVNGISEKDWHLVKQLSEL 136
           MAKLS+TLRLFDAHCHLQDPRIFDKAPQLIS AVDSGVVRFAVNG SEKDWHLVKQLSEL
Sbjct: 1   MAKLSSTLRLFDAHCHLQDPRIFDKAPQLISVAVDSGVVRFAVNGTSEKDWHLVKQLSEL 60

Query: 137 HPCIVPSFGLHPWYVPERTPGWLEKLKEFFEAVPNAAVGEIGLDKGSRGREISFTDQVEV 196
           HPCIVPSFGLHPWYVP RTP WLEKLKEFFEAVP+AAVGEIGLDKGSRGREI+FTDQ+EV
Sbjct: 61  HPCIVPSFGLHPWYVPGRTPVWLEKLKEFFEAVPSAAVGEIGLDKGSRGREINFTDQIEV 120

Query: 197 FQQQLELAKSLEKPASIHCVRAFGDLLQIMKSMGPFPAGFILHSYLGSAQMVPEFSKLGG 256
           FQQQLELAKSLEKPASIHCVRAFGDLL+IMKSMGPFPAGFILHSYLGSA+MVPEFSKLG 
Sbjct: 121 FQQQLELAKSLEKPASIHCVRAFGDLLKIMKSMGPFPAGFILHSYLGSAEMVPEFSKLGA 180

Query: 257 YFSFSGFLMSMDAKKAKKMLKMVPSDRILLETDAPDALPKLKLDSIYFVEGDPSLPEGLK 316
           YFSFSGFLMSM+AKKAKKMLK VPSDRILLETDAPDALPK K +S+Y VEGDP+L E LK
Sbjct: 181 YFSFSGFLMSMEAKKAKKMLKTVPSDRILLETDAPDALPKSKSNSLYLVEGDPALTEFLK 240

Query: 317 TGEQSASTGSEQST-LPSSTDASTLPKETLNHPANIQNVLVYVASLLEISKEELAELSYR 376
             EQSA TGS+QST L SS DASTLP+ETLNHPANIQNVL YVASLL ISKEELAELSYR
Sbjct: 241 AQEQSAPTGSQQSTLLCSSADASTLPRETLNHPANIQNVLDYVASLLGISKEELAELSYR 300

Query: 377 NAEHLFSFKGDTFTVLVFGIVYQEHLEPSND 407
           NAEHLFSFKGD F VLVFGIVYQ+  + SN+
Sbjct: 301 NAEHLFSFKGDAFLVLVFGIVYQQQEKSSNN 331

BLAST of Moc05g02750 vs. NCBI nr
Match: KAG6597222.1 (hypothetical protein SDJN03_10402, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 571.6 bits (1472), Expect = 6.0e-159
Identity = 298/355 (83.94%), Postives = 314/355 (88.45%), Query Frame = 0

Query: 44  CDFPYL------SRLADSPISEIFSKCSRSNFPRFTDT-----GMAKLSATLRLFDAHCH 103
           C+F YL        L    ISE   K +   F + +       GM+KLSATLRLFDAHCH
Sbjct: 15  CEFTYLPLSISHDFLRSWKISEKLCKRALRRFLKRSSASIGRRGMSKLSATLRLFDAHCH 74

Query: 104 LQDPRIFDKAPQLISTAVDSGVVRFAVNGISEKDWHLVKQLSELHPCIVPSFGLHPWYVP 163
           LQDPRIFDKAPQLISTAVDSGV+RFAVNGISEKDWHLVKQLSE HPCI+PSFGLHPWYVP
Sbjct: 75  LQDPRIFDKAPQLISTAVDSGVLRFAVNGISEKDWHLVKQLSESHPCIIPSFGLHPWYVP 134

Query: 164 ERTPGWLEKLKEFFEAVPNAAVGEIGLDKGSRGREISFTDQVEVFQQQLELAKSLEKPAS 223
           ERT GWLEKLKEFFEAVP AAVGEIGLDKGS+G+EI+F DQVEVFQQQLELAKSLE+PAS
Sbjct: 135 ERTTGWLEKLKEFFEAVPTAAVGEIGLDKGSKGKEINFIDQVEVFQQQLELAKSLERPAS 194

Query: 224 IHCVRAFGDLLQIMKSMGPFPAGFILHSYLGSAQMVPEFSKLGGYFSFSGFLMSMDAKKA 283
           IHCVRAFGDLLQIMKSMGPFPAGFILHSYLGSAQMVPEFSKLG YFSFSGFLMSM+AKKA
Sbjct: 195 IHCVRAFGDLLQIMKSMGPFPAGFILHSYLGSAQMVPEFSKLGAYFSFSGFLMSMEAKKA 254

Query: 284 KKMLKMVPSDRILLETDAPDALPKLKLDSIYFVEGDPSLPEGLKTGEQSAS-TGSEQSTL 343
           KKMLKMVPSDRILLETDAPDALPK KLDS+YFVEGDPSLPE LKT EQSA+ TGS+QSTL
Sbjct: 255 KKMLKMVPSDRILLETDAPDALPKSKLDSLYFVEGDPSLPECLKTQEQSAAQTGSQQSTL 314

Query: 344 P-SSTDASTLPKETLNHPANIQNVLVYVASLLEISKEELAELSYRNAEHLFSFKG 386
           P SSTDASTLPK TLNHPANIQNVLVYVASLL+ISKEELAELSYRNAE LFSFKG
Sbjct: 315 PGSSTDASTLPKGTLNHPANIQNVLVYVASLLDISKEELAELSYRNAERLFSFKG 369

BLAST of Moc05g02750 vs. NCBI nr
Match: XP_023538824.1 (uncharacterized protein LOC111799635 [Cucurbita pepo subsp. pepo] >XP_023538825.1 uncharacterized protein LOC111799635 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 566.6 bits (1459), Expect = 1.9e-157
Identity = 286/311 (91.96%), Postives = 299/311 (96.14%), Query Frame = 0

Query: 77  MAKLSATLRLFDAHCHLQDPRIFDKAPQLISTAVDSGVVRFAVNGISEKDWHLVKQLSEL 136
           M+KLSATLRLFDAHCHLQDPRIFDKAPQLISTAVDSGV+RFAVNGISEKDWHLVKQLSEL
Sbjct: 1   MSKLSATLRLFDAHCHLQDPRIFDKAPQLISTAVDSGVLRFAVNGISEKDWHLVKQLSEL 60

Query: 137 HPCIVPSFGLHPWYVPERTPGWLEKLKEFFEAVPNAAVGEIGLDKGSRGREISFTDQVEV 196
           HPCI+PSFGLHPWYVPERT GWLEKLKEFFEAVP AAVGEIGLD+GS+G+EI+F DQVEV
Sbjct: 61  HPCIIPSFGLHPWYVPERTTGWLEKLKEFFEAVPTAAVGEIGLDRGSKGKEINFIDQVEV 120

Query: 197 FQQQLELAKSLEKPASIHCVRAFGDLLQIMKSMGPFPAGFILHSYLGSAQMVPEFSKLGG 256
           F+QQLELAKSLE+PASIHCVRAFGDLLQIMKSMGPFPAGFILHSYLGSAQMVPEFSKLG 
Sbjct: 121 FRQQLELAKSLERPASIHCVRAFGDLLQIMKSMGPFPAGFILHSYLGSAQMVPEFSKLGA 180

Query: 257 YFSFSGFLMSMDAKKAKKMLKMVPSDRILLETDAPDALPKLKLDSIYFVEGDPSLPEGLK 316
           YFSFSGFLMSM+AKKAKKMLKMVPSDRILLETDAPDALPK KLDS+YFVEGDPSLPE LK
Sbjct: 181 YFSFSGFLMSMEAKKAKKMLKMVPSDRILLETDAPDALPKSKLDSLYFVEGDPSLPECLK 240

Query: 317 TGEQSAS-TGSEQSTLP-SSTDASTLPKETLNHPANIQNVLVYVASLLEISKEELAELSY 376
           T EQSA+ TGS+QSTLP SSTDASTLPK TLNHPANIQNVLVYVASLLEISKEELAELSY
Sbjct: 241 TQEQSAAQTGSQQSTLPGSSTDASTLPKGTLNHPANIQNVLVYVASLLEISKEELAELSY 300

Query: 377 RNAEHLFSFKG 386
           RNAE LFSF G
Sbjct: 301 RNAERLFSFTG 311

BLAST of Moc05g02750 vs. NCBI nr
Match: XP_022921728.1 (uncharacterized protein LOC111464387 [Cucurbita moschata] >XP_022964694.1 uncharacterized protein LOC111464387 [Cucurbita moschata])

HSP 1 Score: 565.8 bits (1457), Expect = 3.3e-157
Identity = 286/311 (91.96%), Postives = 298/311 (95.82%), Query Frame = 0

Query: 77  MAKLSATLRLFDAHCHLQDPRIFDKAPQLISTAVDSGVVRFAVNGISEKDWHLVKQLSEL 136
           M+KLSATLRLFDAHCHLQDPRIFDKAPQLISTAVDSGV+RFAVNGISEKDWHLVKQLSE 
Sbjct: 1   MSKLSATLRLFDAHCHLQDPRIFDKAPQLISTAVDSGVLRFAVNGISEKDWHLVKQLSES 60

Query: 137 HPCIVPSFGLHPWYVPERTPGWLEKLKEFFEAVPNAAVGEIGLDKGSRGREISFTDQVEV 196
           HPCI+PSFGLHPWYVPERT GWLEKLKEFFEAVP AAVGEIGLDKGS+G+EI+F DQVEV
Sbjct: 61  HPCIIPSFGLHPWYVPERTTGWLEKLKEFFEAVPTAAVGEIGLDKGSKGKEINFIDQVEV 120

Query: 197 FQQQLELAKSLEKPASIHCVRAFGDLLQIMKSMGPFPAGFILHSYLGSAQMVPEFSKLGG 256
           FQQQLELAKSLE+PASIHCVRAFGDLLQIMKSMGPFPAGFILHSYLGSAQMVPEFSKLG 
Sbjct: 121 FQQQLELAKSLERPASIHCVRAFGDLLQIMKSMGPFPAGFILHSYLGSAQMVPEFSKLGA 180

Query: 257 YFSFSGFLMSMDAKKAKKMLKMVPSDRILLETDAPDALPKLKLDSIYFVEGDPSLPEGLK 316
           YFSFSGFLMSM+AKKAKKMLKMVPSDRILLETDAPDALPK KLDS+YFVEGDPSLPE LK
Sbjct: 181 YFSFSGFLMSMEAKKAKKMLKMVPSDRILLETDAPDALPKSKLDSLYFVEGDPSLPECLK 240

Query: 317 TGEQSAS-TGSEQSTLP-SSTDASTLPKETLNHPANIQNVLVYVASLLEISKEELAELSY 376
           T EQSA+ TGS+QSTLP SSTDASTLPK TLNHPANIQNVLVYVASLL+ISKEELAELSY
Sbjct: 241 TQEQSAAQTGSQQSTLPGSSTDASTLPKGTLNHPANIQNVLVYVASLLDISKEELAELSY 300

Query: 377 RNAEHLFSFKG 386
           RNAE LFSF G
Sbjct: 301 RNAERLFSFTG 311

BLAST of Moc05g02750 vs. ExPASy Swiss-Prot
Match: P37545 (Uncharacterized metal-dependent hydrolase YabD OS=Bacillus subtilis (strain 168) OX=224308 GN=yabD PE=3 SV=1)

HSP 1 Score: 108.2 bits (269), Expect = 2.5e-22
Identity = 84/298 (28.19%), Postives = 129/298 (43.29%), Query Frame = 0

Query: 86  LFDAHCHLQDPRIFDKAPQLISTAVDSGVVRFAVNGISEKDWHLVKQLSELHPCIVPSFG 145
           LFD H HL   +      ++I  A    V R  V G          ++ E +  I  + G
Sbjct: 2   LFDTHAHLNAEQYDTDLEEVIERAKAEKVERIVVVGFDRPTITRAMEMIEEYDFIYAAIG 61

Query: 146 LHPWYVPERTPGWLEKLKEFFEAVPNAAVGEIGLDK--GSRGREISFTDQVEVFQQQLEL 205
            HP    + T   L  +KE        A+GE+GLD       ++I    Q EVF+ Q+ L
Sbjct: 62  WHPVDAIDMTEEDLAWIKELSAHEKVVAIGEMGLDYHWDKSPKDI----QKEVFRNQIAL 121

Query: 206 AKSLEKPASIHCVRAFGDLLQIMKSMGPFPAGFILHSYLGSAQMVPEFSKLGGYFSFSGF 265
           AK +  P  IH   A  D++ I+K  G    G I+H + GSA++  E  K+  Y SF G 
Sbjct: 122 AKEVNLPIIIHNRDATEDVVTILKEEGAEAVGGIMHCFTGSAEVARECMKMNFYLSFGGP 181

Query: 266 LMSMDAKKAKKMLKMVPSDRILLETDAPDALPKLKLDSIYFVEGDPSLPEGLKTGEQSAS 325
           +   +AKK K+++K +P+DR+L+ETD P            F+   P              
Sbjct: 182 VTFKNAKKPKEVVKEIPNDRLLIETDCP------------FLTPHPF------------- 241

Query: 326 TGSEQSTLPSSTDASTLPKETLNHPANIQNVLVYVASLLEISKEELAELSYRNAEHLF 382
                             +   N P+ ++ V   +A L E++ EE+A ++  NA+ LF
Sbjct: 242 ------------------RGKRNEPSYVKYVAEQIAELKEMTFEEIASITTENAKRLF 252

BLAST of Moc05g02750 vs. ExPASy Swiss-Prot
Match: P39408 (Uncharacterized metal-dependent hydrolase YjjV OS=Escherichia coli (strain K12) OX=83333 GN=yjjV PE=1 SV=2)

HSP 1 Score: 95.1 bits (235), Expect = 2.1e-18
Identity = 68/231 (29.44%), Postives = 105/231 (45.45%), Query Frame = 0

Query: 85  RLFDAHCHLQDPRIFDKAPQLISTAVDSGVVRFAVNGISEKDWHLVKQLSELHPCIVPSF 144
           R  D HCH   P         +  A  +GV +  V     +++  V  L+E +  +  + 
Sbjct: 4   RFIDTHCHFDFPPFSGDEEASLQRAAQAGVGKIIVPATEAENFARVLALAENYQPLYAAL 63

Query: 145 GLHPWYVPERTPGWLEKLKEFFEAVPN--AAVGEIGLDKGSRGREISFTDQVEVFQQQLE 204
           GLHP  + + +   LE+L++  E  P    AVGEIGLD    G +  F  Q  +  +QL+
Sbjct: 64  GLHPGMLEKHSDVSLEQLQQALERRPAKVVAVGEIGLD--LFGDDPQFERQQWLLDEQLK 123

Query: 205 LAKSLEKPASIHCVRAFGDLLQIMKSMGPFPAGFILHSYLGSAQMVPEFSKLGGYFSFSG 264
           LAK  + P  +H  R    L   +K     P   ++H + GS Q    F +LG      G
Sbjct: 124 LAKRYDLPVILHSRRTHDKLAMHLK-RHDLPRTGVVHGFSGSLQQAERFVQLGYKIGVGG 183

Query: 265 FLMSMDAKKAKKMLKMVPSDRILLETDAPDALPKLKLDSIYFVEGDPSLPE 314
            +    A K + ++  +P   +LLETDAPD    + L+     +G P+ PE
Sbjct: 184 TITYPRASKTRDVIAKLPLASLLLETDAPD----MPLNGF---QGQPNRPE 224

BLAST of Moc05g02750 vs. ExPASy Swiss-Prot
Match: Q8K9J1 (Uncharacterized metal-dependent hydrolase BUsg_343 OS=Buchnera aphidicola subsp. Schizaphis graminum (strain Sg) OX=198804 GN=BUsg_343 PE=3 SV=1)

HSP 1 Score: 94.0 bits (232), Expect = 4.8e-18
Identity = 61/218 (27.98%), Postives = 106/218 (48.62%), Query Frame = 0

Query: 86  LFDAHCHL--------QDPRIFDKAPQLISTAVDSGVVRFAVNGISEKDWHLVKQLSELH 145
           L D+HCH+         D  +  +  ++++ A ++ V +F     S ++++  K+L   +
Sbjct: 3   LIDSHCHIDQMNIDQKNDDLLHQEIEKILNKAYENHVKKFLTVSTSIQNFYKTKKLLSTY 62

Query: 146 PCIVPSFGLHPWYVPERTPGWLEKLKEFFEAVPNAAVGEIGLDKGSRGREISFTDQVEVF 205
             I  S G+HP Y  ++    L +LK+        A+GE GLD        +   Q + F
Sbjct: 63  DSIFYSCGIHPLYC-QKEIECLNELKKLSYDKCVIALGETGLDYYYSFETKNL--QEKFF 122

Query: 206 QQQLELAKSLEKPASIHCVRAFGDLLQIMKSMGPFPAGFILHSYLGSAQMVPEFSKLGGY 265
           ++ + +A +L KP  IH   A  D ++I+K       G ILHS+  S +   E   +G Y
Sbjct: 123 REHIRIAITLNKPIIIHSRNAIKDTIKILKEENAEKCGGILHSFTESEKSAFELLDMGFY 182

Query: 266 FSFSGFLMSMDAKKAKKMLKMVPSDRILLETDAPDALP 296
            SFSG +    + +    LK +P +++L+ETD+P   P
Sbjct: 183 ISFSGIITFKKSIELCNTLKKIPLEKLLIETDSPYLAP 217

BLAST of Moc05g02750 vs. ExPASy Swiss-Prot
Match: P73335 (D-aminoacyl-tRNA deacylase OS=Synechocystis sp. (strain PCC 6803 / Kazusa) OX=1111708 GN=dtd3 PE=1 SV=1)

HSP 1 Score: 90.5 bits (223), Expect = 5.3e-17
Identity = 75/301 (24.92%), Postives = 126/301 (41.86%), Query Frame = 0

Query: 84  LRLFDAHCHLQDPRIFDKAPQLISTAVDSGVVRFAVNGISEKDWHLVKQLSELHPCIVPS 143
           + L D H H+          QL      +GVV+   + +  +++  ++ L++  P +  +
Sbjct: 1   MHLVDTHVHINFDVFAADLDQLQHRWRQAGVVQLVHSCVKPQEFDQIQSLADRFPELFFA 60

Query: 144 FGLHPWYVPERTPGWLEKLKEFFEAVPN-AAVGEIGLDKGSRGREISFTDQVEVFQQQLE 203
            GLHP    +       ++  + +A     A+GE+GLD            Q+EVF+ QL 
Sbjct: 61  VGLHPLDAEDWQDNTAGQILAYAKADDRVVAIGEMGLDFFKADNR---DHQIEVFRAQLA 120

Query: 204 LAKSLEKPASIHCVRAFGDLLQIMKSM--GPFPAGFILHSYLGSAQMVPEFSKLGGYFSF 263
           +A+ L KP  IHC  A   + Q++        P   ++H + G+ +    F  LG Y SF
Sbjct: 121 IARELNKPVIIHCRDAAQTMRQVLTDFQAESGPVAGVMHCWGGTPEETQWFLDLGFYISF 180

Query: 264 SGFLMSMDAKKAKKMLKMVPSDRILLETDAPDALPKLKLDSIYFVEGDPSLPEGLKTGEQ 323
           SG +    A+  +   +MVP DR+L+ETD P   P               +P+  K    
Sbjct: 181 SGTVTFKKAEGIQASAQMVPPDRLLVETDCPFLAP---------------VPQRGKR--- 240

Query: 324 SASTGSEQSTLPSSTDASTLPKETLNHPANIQNVLVYVASLLEISKEELAELSYRNAEHL 382
                                    N PA +++V   +A+L  +  E LA+ +  NA +L
Sbjct: 241 -------------------------NEPAFVRHVAEAIAALRHVPLETLAQQTTTNARNL 255

BLAST of Moc05g02750 vs. ExPASy Swiss-Prot
Match: E6WHK1 (3'-5' ssDNA/RNA exonuclease TatD OS=Pantoea sp. (strain At-9b) OX=592316 GN=tatD PE=3 SV=1)

HSP 1 Score: 90.1 bits (222), Expect = 6.9e-17
Identity = 58/214 (27.10%), Postives = 102/214 (47.66%), Query Frame = 0

Query: 86  LFDAHCHLQDPRIFDKAPQLISTAVDSGVVRFAVNGISEKDWHLVKQLSELHPCIV-PSF 145
           +FD   +L   +      Q+++ A D+GV    + G +  +    ++L+E HP     + 
Sbjct: 1   MFDIGVNLTSTQFAKDREQVVARARDAGVTGLLITGTNALESQQAQRLAEWHPGYCWSTA 60

Query: 146 GLHPWYVPERTPGWLEKLKEFFEAVPNAAVGEIGLDKGSRGREISFTDQVE-VFQQQLEL 205
           G+HP +    +      L+   E+    A+GE GLD     R  S  DQ E  F  QL+L
Sbjct: 61  GVHPHHASAWSAETANTLRRLAESEQVVAIGECGLD---FNRNFSAHDQQEYAFDAQLQL 120

Query: 206 AKSLEKPASIHCVRAFGDLLQIMKSMGPFPAGFILHSYLGSAQMVPEFSKLGGYFSFSGF 265
           A  L+ P  +HC  A      I++   P   G + H + G+ + +     +G     +G+
Sbjct: 121 AAELQLPVFLHCREAHDRFAAILQPWLPKLVGAVAHCFTGTREELEACLAMGLSIGITGW 180

Query: 266 LM-SMDAKKAKKMLKMVPSDRILLETDAPDALPK 297
           +       + +++L ++P++R+LLETDAP  LP+
Sbjct: 181 VCDERRGMELRELLPLIPAERLLLETDAPWLLPR 211

BLAST of Moc05g02750 vs. ExPASy TrEMBL
Match: A0A6J1CYM1 (uncharacterized protein LOC111015984 OS=Momordica charantia OX=3673 GN=LOC111015984 PE=4 SV=1)

HSP 1 Score: 617.5 bits (1591), Expect = 4.6e-173
Identity = 309/309 (100.00%), Postives = 309/309 (100.00%), Query Frame = 0

Query: 77  MAKLSATLRLFDAHCHLQDPRIFDKAPQLISTAVDSGVVRFAVNGISEKDWHLVKQLSEL 136
           MAKLSATLRLFDAHCHLQDPRIFDKAPQLISTAVDSGVVRFAVNGISEKDWHLVKQLSEL
Sbjct: 1   MAKLSATLRLFDAHCHLQDPRIFDKAPQLISTAVDSGVVRFAVNGISEKDWHLVKQLSEL 60

Query: 137 HPCIVPSFGLHPWYVPERTPGWLEKLKEFFEAVPNAAVGEIGLDKGSRGREISFTDQVEV 196
           HPCIVPSFGLHPWYVPERTPGWLEKLKEFFEAVPNAAVGEIGLDKGSRGREISFTDQVEV
Sbjct: 61  HPCIVPSFGLHPWYVPERTPGWLEKLKEFFEAVPNAAVGEIGLDKGSRGREISFTDQVEV 120

Query: 197 FQQQLELAKSLEKPASIHCVRAFGDLLQIMKSMGPFPAGFILHSYLGSAQMVPEFSKLGG 256
           FQQQLELAKSLEKPASIHCVRAFGDLLQIMKSMGPFPAGFILHSYLGSAQMVPEFSKLGG
Sbjct: 121 FQQQLELAKSLEKPASIHCVRAFGDLLQIMKSMGPFPAGFILHSYLGSAQMVPEFSKLGG 180

Query: 257 YFSFSGFLMSMDAKKAKKMLKMVPSDRILLETDAPDALPKLKLDSIYFVEGDPSLPEGLK 316
           YFSFSGFLMSMDAKKAKKMLKMVPSDRILLETDAPDALPKLKLDSIYFVEGDPSLPEGLK
Sbjct: 181 YFSFSGFLMSMDAKKAKKMLKMVPSDRILLETDAPDALPKLKLDSIYFVEGDPSLPEGLK 240

Query: 317 TGEQSASTGSEQSTLPSSTDASTLPKETLNHPANIQNVLVYVASLLEISKEELAELSYRN 376
           TGEQSASTGSEQSTLPSSTDASTLPKETLNHPANIQNVLVYVASLLEISKEELAELSYRN
Sbjct: 241 TGEQSASTGSEQSTLPSSTDASTLPKETLNHPANIQNVLVYVASLLEISKEELAELSYRN 300

Query: 377 AEHLFSFKG 386
           AEHLFSFKG
Sbjct: 301 AEHLFSFKG 309

BLAST of Moc05g02750 vs. ExPASy TrEMBL
Match: A0A5A7U6E3 (Putative metal-dependent hydrolase YabD isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold434G003580 PE=4 SV=1)

HSP 1 Score: 577.4 bits (1487), Expect = 5.3e-161
Identity = 292/331 (88.22%), Postives = 307/331 (92.75%), Query Frame = 0

Query: 77  MAKLSATLRLFDAHCHLQDPRIFDKAPQLISTAVDSGVVRFAVNGISEKDWHLVKQLSEL 136
           MAKLS+TLRLFDAHCHLQDPRIFDKAPQLIS AVDSGVVRFAVNG SEKDWHLVKQLSEL
Sbjct: 1   MAKLSSTLRLFDAHCHLQDPRIFDKAPQLISVAVDSGVVRFAVNGTSEKDWHLVKQLSEL 60

Query: 137 HPCIVPSFGLHPWYVPERTPGWLEKLKEFFEAVPNAAVGEIGLDKGSRGREISFTDQVEV 196
           HPCIVPSFGLHPWYVP RTP WLEKLKEFFEAVP+AAVGEIGLDKGSRGREI+FTDQ+EV
Sbjct: 61  HPCIVPSFGLHPWYVPGRTPVWLEKLKEFFEAVPSAAVGEIGLDKGSRGREINFTDQIEV 120

Query: 197 FQQQLELAKSLEKPASIHCVRAFGDLLQIMKSMGPFPAGFILHSYLGSAQMVPEFSKLGG 256
           FQQQLELAKSLEKPASIHCVRAFGDLL+IMKSMGPFPAGFILHSYLGSA+MVPEFSKLG 
Sbjct: 121 FQQQLELAKSLEKPASIHCVRAFGDLLKIMKSMGPFPAGFILHSYLGSAEMVPEFSKLGA 180

Query: 257 YFSFSGFLMSMDAKKAKKMLKMVPSDRILLETDAPDALPKLKLDSIYFVEGDPSLPEGLK 316
           YFSFSGFLMSM+AKKAKKMLK VPSDRILLETDAPDALPK K +S+Y VEGDP+L E LK
Sbjct: 181 YFSFSGFLMSMEAKKAKKMLKTVPSDRILLETDAPDALPKSKSNSLYLVEGDPALTEFLK 240

Query: 317 TGEQSASTGSEQST-LPSSTDASTLPKETLNHPANIQNVLVYVASLLEISKEELAELSYR 376
             EQSA TGS+QST L SS DASTLP+ETLNHPANIQNVL YVASLL ISKEELAELSYR
Sbjct: 241 AQEQSAPTGSQQSTLLCSSADASTLPRETLNHPANIQNVLDYVASLLGISKEELAELSYR 300

Query: 377 NAEHLFSFKGDTFTVLVFGIVYQEHLEPSND 407
           NAEHLFSFKGD F VLVFGIVYQ+  + SN+
Sbjct: 301 NAEHLFSFKGDAFLVLVFGIVYQQQEKSSNN 331

BLAST of Moc05g02750 vs. ExPASy TrEMBL
Match: A0A6J1HP04 (uncharacterized protein LOC111464387 OS=Cucurbita moschata OX=3662 GN=LOC111464387 PE=4 SV=1)

HSP 1 Score: 565.8 bits (1457), Expect = 1.6e-157
Identity = 286/311 (91.96%), Postives = 298/311 (95.82%), Query Frame = 0

Query: 77  MAKLSATLRLFDAHCHLQDPRIFDKAPQLISTAVDSGVVRFAVNGISEKDWHLVKQLSEL 136
           M+KLSATLRLFDAHCHLQDPRIFDKAPQLISTAVDSGV+RFAVNGISEKDWHLVKQLSE 
Sbjct: 1   MSKLSATLRLFDAHCHLQDPRIFDKAPQLISTAVDSGVLRFAVNGISEKDWHLVKQLSES 60

Query: 137 HPCIVPSFGLHPWYVPERTPGWLEKLKEFFEAVPNAAVGEIGLDKGSRGREISFTDQVEV 196
           HPCI+PSFGLHPWYVPERT GWLEKLKEFFEAVP AAVGEIGLDKGS+G+EI+F DQVEV
Sbjct: 61  HPCIIPSFGLHPWYVPERTTGWLEKLKEFFEAVPTAAVGEIGLDKGSKGKEINFIDQVEV 120

Query: 197 FQQQLELAKSLEKPASIHCVRAFGDLLQIMKSMGPFPAGFILHSYLGSAQMVPEFSKLGG 256
           FQQQLELAKSLE+PASIHCVRAFGDLLQIMKSMGPFPAGFILHSYLGSAQMVPEFSKLG 
Sbjct: 121 FQQQLELAKSLERPASIHCVRAFGDLLQIMKSMGPFPAGFILHSYLGSAQMVPEFSKLGA 180

Query: 257 YFSFSGFLMSMDAKKAKKMLKMVPSDRILLETDAPDALPKLKLDSIYFVEGDPSLPEGLK 316
           YFSFSGFLMSM+AKKAKKMLKMVPSDRILLETDAPDALPK KLDS+YFVEGDPSLPE LK
Sbjct: 181 YFSFSGFLMSMEAKKAKKMLKMVPSDRILLETDAPDALPKSKLDSLYFVEGDPSLPECLK 240

Query: 317 TGEQSAS-TGSEQSTLP-SSTDASTLPKETLNHPANIQNVLVYVASLLEISKEELAELSY 376
           T EQSA+ TGS+QSTLP SSTDASTLPK TLNHPANIQNVLVYVASLL+ISKEELAELSY
Sbjct: 241 TQEQSAAQTGSQQSTLPGSSTDASTLPKGTLNHPANIQNVLVYVASLLDISKEELAELSY 300

Query: 377 RNAEHLFSFKG 386
           RNAE LFSF G
Sbjct: 301 RNAERLFSFTG 311

BLAST of Moc05g02750 vs. ExPASy TrEMBL
Match: A0A0A0L4G2 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G127010 PE=4 SV=1)

HSP 1 Score: 562.4 bits (1448), Expect = 1.8e-156
Identity = 284/311 (91.32%), Postives = 294/311 (94.53%), Query Frame = 0

Query: 76  GMAKLSATLRLFDAHCHLQDPRIFDKAPQLISTAVDSGVVRFAVNGISEKDWHLVKQLSE 135
           GMAKLS+TLRLFDAHCHLQDPRIFDKAPQLIS AVDSGVVRFAVNGISEKDWHLVKQLSE
Sbjct: 44  GMAKLSSTLRLFDAHCHLQDPRIFDKAPQLISAAVDSGVVRFAVNGISEKDWHLVKQLSE 103

Query: 136 LHPCIVPSFGLHPWYVPERTPGWLEKLKEFFEAVPNAAVGEIGLDKGSRGREISFTDQVE 195
           LHPCIVPSFGLHPWYVP RTPGWLEKLKEFFEAVP+AAVGEIGLDKGSRGREI+FTDQ+E
Sbjct: 104 LHPCIVPSFGLHPWYVPGRTPGWLEKLKEFFEAVPSAAVGEIGLDKGSRGREINFTDQIE 163

Query: 196 VFQQQLELAKSLEKPASIHCVRAFGDLLQIMKSMGPFPAGFILHSYLGSAQMVPEFSKLG 255
           VFQQQLELAKSLEKPASIHCVRAFGDLL IMKSMGPFPAGFILHSYLGSA+MVPEFSKLG
Sbjct: 164 VFQQQLELAKSLEKPASIHCVRAFGDLLNIMKSMGPFPAGFILHSYLGSAEMVPEFSKLG 223

Query: 256 GYFSFSGFLMSMDAKKAKKMLKMVPSDRILLETDAPDALPKLKLDSIYFVEGDPSLPEGL 315
            YFSFSGFLMSM+AKKAKKMLKMVPSDRILLETDAPDALPK K +S+Y VEGDPSL E L
Sbjct: 224 AYFSFSGFLMSMEAKKAKKMLKMVPSDRILLETDAPDALPKSKSNSLYLVEGDPSLTEFL 283

Query: 316 KTGEQSASTGSEQST-LPSSTDASTLPKETLNHPANIQNVLVYVASLLEISKEELAELSY 375
           K  EQSA TGS+QST L SSTDASTLP+ETLNHPANIQNVL YVASLL ISKEELAELSY
Sbjct: 284 KAQEQSAPTGSQQSTLLCSSTDASTLPRETLNHPANIQNVLDYVASLLGISKEELAELSY 343

Query: 376 RNAEHLFSFKG 386
           RNAEHLFSF G
Sbjct: 344 RNAEHLFSFNG 354

BLAST of Moc05g02750 vs. ExPASy TrEMBL
Match: A0A6J1IGS5 (uncharacterized protein LOC111472748 OS=Cucurbita maxima OX=3661 GN=LOC111472748 PE=4 SV=1)

HSP 1 Score: 559.7 bits (1441), Expect = 1.1e-155
Identity = 282/311 (90.68%), Postives = 298/311 (95.82%), Query Frame = 0

Query: 77  MAKLSATLRLFDAHCHLQDPRIFDKAPQLISTAVDSGVVRFAVNGISEKDWHLVKQLSEL 136
           M+KLSATLRLFDAHCHLQDPRIFDKAPQLISTAVDSGV+RFAVNGISEKDWHLVKQLSEL
Sbjct: 1   MSKLSATLRLFDAHCHLQDPRIFDKAPQLISTAVDSGVLRFAVNGISEKDWHLVKQLSEL 60

Query: 137 HPCIVPSFGLHPWYVPERTPGWLEKLKEFFEAVPNAAVGEIGLDKGSRGREISFTDQVEV 196
           HPCI+PSFGLHPW VPERT GWLEKLKEFFEAVP AAVGEIGLDKGS+G+EI+F DQVEV
Sbjct: 61  HPCIIPSFGLHPWCVPERTTGWLEKLKEFFEAVPTAAVGEIGLDKGSKGKEINFIDQVEV 120

Query: 197 FQQQLELAKSLEKPASIHCVRAFGDLLQIMKSMGPFPAGFILHSYLGSAQMVPEFSKLGG 256
           F+QQLELAKSLE+PASIHCVRAFGDLLQIMKSMGPFPAGFILHSYLGSAQMVPEF+KLG 
Sbjct: 121 FRQQLELAKSLERPASIHCVRAFGDLLQIMKSMGPFPAGFILHSYLGSAQMVPEFTKLGA 180

Query: 257 YFSFSGFLMSMDAKKAKKMLKMVPSDRILLETDAPDALPKLKLDSIYFVEGDPSLPEGLK 316
           YFSFSGFLMSM+AKKAKKMLKMVPSDRILLETDAPDALPK K+DS+YFVEGDPSLPE LK
Sbjct: 181 YFSFSGFLMSMEAKKAKKMLKMVPSDRILLETDAPDALPKSKVDSLYFVEGDPSLPECLK 240

Query: 317 TGEQSAS-TGSEQSTLP-SSTDASTLPKETLNHPANIQNVLVYVASLLEISKEELAELSY 376
           T EQSA+ TGS+QST+P SSTDASTLPK TLNHPANIQNVLVYVASLL+ISKEELAELSY
Sbjct: 241 TQEQSAAQTGSQQSTVPGSSTDASTLPKGTLNHPANIQNVLVYVASLLDISKEELAELSY 300

Query: 377 RNAEHLFSFKG 386
           RNAE LFSF G
Sbjct: 301 RNAERLFSFTG 311

BLAST of Moc05g02750 vs. TAIR 10
Match: AT5G17570.1 (TatD related DNase )

HSP 1 Score: 423.3 bits (1087), Expect = 2.4e-118
Identity = 206/302 (68.21%), Postives = 246/302 (81.46%), Query Frame = 0

Query: 84  LRLFDAHCHLQDPRIFDKAPQLISTAVDSGVVRFAVNGISEKDWHLVKQLSELHPCIVPS 143
           ++LFDAHCHLQDPRI  KAPQ+IS+AV SGV  FAVNG+SEKDW LVK++   +P +VP 
Sbjct: 1   MKLFDAHCHLQDPRIITKAPQIISSAVASGVSAFAVNGVSEKDWSLVKEMGAKYPSVVPC 60

Query: 144 FGLHPWYVPERTPGWLEKLKEFFEAVPNAAVGEIGLDKGSRGREISFTDQVEVFQQQLEL 203
           FG+HPWYV ER+P W E LK FFE  P AAVGEIGLDKGS+GREI F++QV VF+QQLEL
Sbjct: 61  FGIHPWYVAERSPQWFETLKSFFETTPTAAVGEIGLDKGSKGREIDFSEQVTVFRQQLEL 120

Query: 204 AKSLEKPASIHCVRAFGDLLQIMKSMGPFPAGFILHSYLGSAQMVPEFSKLGGYFSFSGF 263
           AK L+KPAS+HCVRAFGDLL+I+KS+GPFP+G +LHSYLGSA+MVPEF+KLG YFSFSGF
Sbjct: 121 AKELKKPASVHCVRAFGDLLEILKSVGPFPSGVMLHSYLGSAEMVPEFAKLGAYFSFSGF 180

Query: 264 LMSMDAKKAKKMLKMVPSDRILLETDAPDALPKLKLDSIYFVEGDPSLPEGLKTGEQSAS 323
           LMSM  KKAKKMLK VPSDRILLETD+PDALPK +   +YFV+GDPSLPE   + +   S
Sbjct: 181 LMSMSEKKAKKMLKAVPSDRILLETDSPDALPKAESGCLYFVDGDPSLPEEGNSAQDLDS 240

Query: 324 TGSEQSTLPSSTDASTLPKETLNHPANIQNVLVYVASLLEISKEELAELSYRNAEHLFSF 383
              ++  +  S+D+  L KETLNHPANI  VL YVA LL++  EELAELSY+NA  LFS+
Sbjct: 241 ASYDKPNV--SSDSMKLTKETLNHPANIHIVLGYVAQLLDMKNEELAELSYQNAVRLFSY 300

Query: 384 KG 386
           +G
Sbjct: 301 EG 300

BLAST of Moc05g02750 vs. TAIR 10
Match: AT3G03500.1 (TatD related DNase )

HSP 1 Score: 327.0 bits (837), Expect = 2.4e-89
Identity = 163/302 (53.97%), Postives = 206/302 (68.21%), Query Frame = 0

Query: 84  LRLFDAHCHLQDPRIFDKAPQLISTAVDSGVVRFAVNGISEKDWHLVKQLSELHPCIVPS 143
           ++LFDAHCHLQD R+ DKA QLIS A+  GV  FAVNG SEKDW+LVK++ E +P +VP 
Sbjct: 1   MKLFDAHCHLQDKRVIDKASQLISAALAVGVTNFAVNGTSEKDWNLVKEMGETYPSVVPC 60

Query: 144 FGLHPWYVPERTPGWLEKLKEFFEAVPNAAVGEIGLDKGSRGREISFTDQVEVFQQQLEL 203
           FGLHPW++ +R+P W + LK+FFE  P AAVGEIGLDKG     I ++DQ+ VF+ QLEL
Sbjct: 61  FGLHPWFIADRSPHWFKTLKKFFETTPTAAVGEIGLDKGPLAGGIDYSDQLVVFRPQLEL 120

Query: 204 AKSLEKPASIHCVRAFGDLLQIMKSMGPFPAGFILHSYLGSAQMVPEFSKLGGYFSFSGF 263
           AK L KP ++HC+ AF DLL+IM+S+GPFPAG ILHS+ GSA++VP+ ++LG YFSFSG+
Sbjct: 121 AKELNKPVAVHCIDAFDDLLEIMRSIGPFPAGVILHSFNGSAEVVPKLAELGAYFSFSGW 180

Query: 264 LMSMDAKKAKKMLKMVPSDRILLETDAPDALPKLKLDSIYFVEGDPSLPEGLKTGEQSAS 323
              +D K AKK LK +PSDR+LLETD+PD LPK                           
Sbjct: 181 FTYIDEKIAKKTLKSIPSDRLLLETDSPDGLPK--------------------------- 240

Query: 324 TGSEQSTLPSSTDASTLPKETLNHPANIQNVLVYVASLLEISKEELAELSYRNAEHLFSF 383
                     S ++S+ PK TLN PANI  VL YVA+L  + KEELAELSY N+  LFS+
Sbjct: 241 ----------SDESSSDPKPTLNEPANILAVLDYVANLSNMKKEELAELSYVNSVRLFSY 265

Query: 384 KG 386
            G
Sbjct: 301 PG 265

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022146895.19.5e-173100.00uncharacterized protein LOC111015984 [Momordica charantia] >XP_022146896.1 uncha... [more]
KAA0049069.11.1e-16088.22putative metal-dependent hydrolase YabD isoform X1 [Cucumis melo var. makuwa] >T... [more]
KAG6597222.16.0e-15983.94hypothetical protein SDJN03_10402, partial [Cucurbita argyrosperma subsp. sorori... [more]
XP_023538824.11.9e-15791.96uncharacterized protein LOC111799635 [Cucurbita pepo subsp. pepo] >XP_023538825.... [more]
XP_022921728.13.3e-15791.96uncharacterized protein LOC111464387 [Cucurbita moschata] >XP_022964694.1 unchar... [more]
Match NameE-valueIdentityDescription
P375452.5e-2228.19Uncharacterized metal-dependent hydrolase YabD OS=Bacillus subtilis (strain 168)... [more]
P394082.1e-1829.44Uncharacterized metal-dependent hydrolase YjjV OS=Escherichia coli (strain K12) ... [more]
Q8K9J14.8e-1827.98Uncharacterized metal-dependent hydrolase BUsg_343 OS=Buchnera aphidicola subsp.... [more]
P733355.3e-1724.92D-aminoacyl-tRNA deacylase OS=Synechocystis sp. (strain PCC 6803 / Kazusa) OX=11... [more]
E6WHK16.9e-1727.103'-5' ssDNA/RNA exonuclease TatD OS=Pantoea sp. (strain At-9b) OX=592316 GN=tatD... [more]
Match NameE-valueIdentityDescription
A0A6J1CYM14.6e-173100.00uncharacterized protein LOC111015984 OS=Momordica charantia OX=3673 GN=LOC111015... [more]
A0A5A7U6E35.3e-16188.22Putative metal-dependent hydrolase YabD isoform X1 OS=Cucumis melo var. makuwa O... [more]
A0A6J1HP041.6e-15791.96uncharacterized protein LOC111464387 OS=Cucurbita moschata OX=3662 GN=LOC1114643... [more]
A0A0A0L4G21.8e-15691.32Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G127010 PE=4 SV=1[more]
A0A6J1IGS51.1e-15590.68uncharacterized protein LOC111472748 OS=Cucurbita maxima OX=3661 GN=LOC111472748... [more]
Match NameE-valueIdentityDescription
AT5G17570.12.4e-11868.21TatD related DNase [more]
AT3G03500.12.4e-8953.97TatD related DNase [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (OHB3-1) v2
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableGENE3D3.20.20.140coord: 77..384
e-value: 2.3E-71
score: 241.9
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 316..343
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 314..343
NoneNo IPR availablePANTHERPTHR47176:SF1OSJNBA0020J04.13 PROTEINcoord: 84..385
NoneNo IPR availablePANTHERPTHR47176OSJNBA0020J04.13 PROTEINcoord: 84..385
IPR001130TatD familyPFAMPF01026TatD_DNasecoord: 87..382
e-value: 1.7E-69
score: 234.1
IPR001130TatD familyCDDcd01310TatD_DNAsecoord: 86..382
e-value: 1.12677E-65
score: 209.737
IPR032466Metal-dependent hydrolaseSUPERFAMILY51556Metallo-dependent hydrolasescoord: 84..382

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Moc05g02750.1Moc05g02750.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0016788 hydrolase activity, acting on ester bonds