Moc05g02110 (gene) Bitter gourd (OHB3-1) v2

Overview


Name	Moc05g02110
Type	gene
Organism	Momordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
Description	Chaperone protein dnaJ 20
Location	chr5: 1417486 .. 1418729 (+)
RNA-Seq Expression	Moc05g02110
Synteny	Moc05g02110

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGCAGTGCTCCGATTTTACCTTATCAGGAAGCGAATCCCGCTTCTACATCCCCTCGAACCCTAGCATTACCGGCCGGCGGTCCTTTTCCGGCCACCGATCTCGGATTTTCTTCCACAGTTCCACGCGCTTCCCTTCTCTTTCGATCAAAGCCAAGGCCTCCTTCAACGAAGGGATCGTCTCCGGGGTCGCCGATGGCTCCTTCTACGATTTGCTCGGAATTTCTCAGTCCGGATCCTTGACGGAAATCAAGCGCGCGTACAAGCAGCTCGCCCGGAAGTACCATCCGGACGTGTCGCCGCCGGGCCGGGCGGAGGAATATACCAAGAGGTTCATTCGCGTACAGGAGGCGTACGAGACATTGTCCGATCCGAGGAGGCGAGCGCTGTATGACAGAGACATGGTTAGAGGCCTTCAAGTCGCGTTCTCTGCTCGGAGACGATACGAGGCCGATGAGGTATTGAATCAGATTATCGATTCTCGTTTGTATTATCGATTTTTTAGGGTTAATTGCGTTTGATTGTTGATGGATTTCGGATCTTATGTGTTTTTTCTTTTGCCCGCCTTGTTTTTGTGGTAATTCTGTGTTCAATTTCTCGGATGCTATTCCGAGAGAAGATAAGTGAAGGATTTGATCTAATTCTCAGATGTTTATTTGATTTTAGATCTTGTGATAGTGAAGAAATAAAACGCCGTTTTATTCTGGCTCCTGGTTCTTTGTTTCTTTCCTCTTTGTTTTTCCCTCTGTCTCTATCTGAGAACAGACGCGGGAGGATCGATAGAAAGCTGTTTCTCTTTGGAGCAAGGATTTTCCATTGGTTTATTCAGTGCTTTGTTCTGCAATGGCTGCTGTTTTCTTGATAATTTACTCCAAATATGGAAACTCGTTATAATAAATTTTAATTTTCAATATACTGCCATGCCAGTTTGCAGTTTTTCTCATGATCTAGTCGTGTTCTGGCACCTCTGAAAGTTGTTCGTCTATGTTTCCCAGAAATCCTTTTCCCCTCTCAGTGAAACCATACTTCTCATTAGCACACTTGCCAATTTTACTTTTCCATCTAGCATCATAATATTGAATCTTCCGATTTGATTTTGGTTCAGGGAGTTCCGGAGAGAAGTGGATGGAAATACTGTTGGCAAGCTCAGCTCTCAGAATTGAAAAGAAGAAGCATGGACAAGGATGTAAGAGTCAATAGCTCCTGGGGAGCTCGAATGCGCCGGCAAATGAATGAACAGGTCTGA

mRNA sequence

ATGCAGTGCTCCGATTTTACCTTATCAGGAAGCGAATCCCGCTTCTACATCCCCTCGAACCCTAGCATTACCGGCCGGCGGTCCTTTTCCGGCCACCGATCTCGGATTTTCTTCCACAGTTCCACGCGCTTCCCTTCTCTTTCGATCAAAGCCAAGGCCTCCTTCAACGAAGGGATCGTCTCCGGGGTCGCCGATGGCTCCTTCTACGATTTGCTCGGAATTTCTCAGTCCGGATCCTTGACGGAAATCAAGCGCGCGTACAAGCAGCTCGCCCGGAAGTACCATCCGGACGTGTCGCCGCCGGGCCGGGCGGAGGAATATACCAAGAGGTTCATTCGCGTACAGGAGGCGTACGAGACATTGTCCGATCCGAGGAGGCGAGCGCTGTATGACAGAGACATGGTTAGAGGCCTTCAAGTCGCGTTCTCTGCTCGGAGACGATACGAGGCCGATGAGGGAGTTCCGGAGAGAAGTGGATGGAAATACTGTTGGCAAGCTCAGCTCTCAGAATTGAAAAGAAGAAGCATGGACAAGGATGTAAGAGTCAATAGCTCCTGGGGAGCTCGAATGCGCCGGCAAATGAATGAACAGGTCTGA

Coding sequence (CDS)

ATGCAGTGCTCCGATTTTACCTTATCAGGAAGCGAATCCCGCTTCTACATCCCCTCGAACCCTAGCATTACCGGCCGGCGGTCCTTTTCCGGCCACCGATCTCGGATTTTCTTCCACAGTTCCACGCGCTTCCCTTCTCTTTCGATCAAAGCCAAGGCCTCCTTCAACGAAGGGATCGTCTCCGGGGTCGCCGATGGCTCCTTCTACGATTTGCTCGGAATTTCTCAGTCCGGATCCTTGACGGAAATCAAGCGCGCGTACAAGCAGCTCGCCCGGAAGTACCATCCGGACGTGTCGCCGCCGGGCCGGGCGGAGGAATATACCAAGAGGTTCATTCGCGTACAGGAGGCGTACGAGACATTGTCCGATCCGAGGAGGCGAGCGCTGTATGACAGAGACATGGTTAGAGGCCTTCAAGTCGCGTTCTCTGCTCGGAGACGATACGAGGCCGATGAGGGAGTTCCGGAGAGAAGTGGATGGAAATACTGTTGGCAAGCTCAGCTCTCAGAATTGAAAAGAAGAAGCATGGACAAGGATGTAAGAGTCAATAGCTCCTGGGGAGCTCGAATGCGCCGGCAAATGAATGAACAGGTCTGA

Protein sequence

MQCSDFTLSGSESRFYIPSNPSITGRRSFSGHRSRIFFHSSTRFPSLSIKAKASFNEGIVSGVADGSFYDLLGISQSGSLTEIKRAYKQLARKYHPDVSPPGRAEEYTKRFIRVQEAYETLSDPRRRALYDRDMVRGLQVAFSARRRYEADEGVPERSGWKYCWQAQLSELKRRSMDKDVRVNSSWGARMRRQMNEQV

Homology

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BLAST of Moc05g02110 vs. NCBI nr
Match: XP_008438327.1 (PREDICTED: chaperone protein dnaJ 20, chloroplastic-like [Cucumis melo])

HSP 1 Score: 317.4 bits (812), Expect = 8.8e-83
Identity = 162/204 (79.41%), Postives = 179/204 (87.75%), Query Frame = 0


Query: 1   MQCSDFTLSGSESRFYIPSNPSITGRRSFSGHRSRIFF-----HSSTRFPSLSIKAKASF 60
           MQCSDFT SGS+SRFYIPSNP+I+ RR  SG+RSR+FF      +STR PSLSI+AKASF
Sbjct: 27  MQCSDFTFSGSDSRFYIPSNPTISARRPISGYRSRVFFPHTPPFNSTRLPSLSIRAKASF 86

Query: 61  NEGIVSG-VADGSFYDLLGISQSGSLTEIKRAYKQLARKYHPDVSPPGRAEEYTKRFIRV 120
           NEG VS  VA+GSFYDLLGISQSGSL EIKRAYKQLARKYHPDVSPPG AEEYTK FIRV
Sbjct: 87  NEGFVSSEVAEGSFYDLLGISQSGSLAEIKRAYKQLARKYHPDVSPPGGAEEYTKMFIRV 146

Query: 121 QEAYETLSDPRRRALYDRDMVRGLQVAFSARRRYEADEGVPERSGWKYCWQAQLSELKRR 180
           QEAYETLSDPRRRALYDRDM+ GLQVAFSARRRY+A E V E+SGW+  W+AQ+SELKRR
Sbjct: 147 QEAYETLSDPRRRALYDRDMIGGLQVAFSARRRYDAAEEVAEKSGWRNSWEAQISELKRR 206

Query: 181 SMDKDVRVNSSWGARMRRQMNEQV 199
           SM+KD+R N SWGARMRRQMNEQ+
Sbjct: 207 SMEKDLRQNMSWGARMRRQMNEQI 230

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BLAST of Moc05g02110 vs. NCBI nr
Match: XP_022974737.1 (chaperone protein dnaJ 20, chloroplastic-like [Cucurbita maxima])

HSP 1 Score: 315.8 bits (808), Expect = 2.6e-82
Identity = 162/204 (79.41%), Postives = 176/204 (86.27%), Query Frame = 0


Query: 1   MQCSDFTLSGSESRFYIPSNPSITGRRSFSGHRSRIFFH-----SSTRFPSLSIKAKASF 60
           MQCSDFT SG + R  IPS+PSITGRR  SG +SR+FF      +STR PSLSI+AKASF
Sbjct: 1   MQCSDFTYSGGDYRILIPSSPSITGRRQISGQKSRVFFPNTPPCNSTRLPSLSIRAKASF 60

Query: 61  NEGIVS-GVADGSFYDLLGISQSGSLTEIKRAYKQLARKYHPDVSPPGRAEEYTKRFIRV 120
           N GI S   ADGSFYDLLGIS+SGSL EIKRAYKQLARKYHPDVSPPGRAEEYTKRFIRV
Sbjct: 61  NGGIASFEAADGSFYDLLGISESGSLAEIKRAYKQLARKYHPDVSPPGRAEEYTKRFIRV 120

Query: 121 QEAYETLSDPRRRALYDRDMVRGLQVAFSARRRYEADEGVPERSGWKYCWQAQLSELKRR 180
           QEAYETLSDPRRRALYDRDMV GLQVAFSARRRY+ DEGVP++S WK CW+AQ+SELKRR
Sbjct: 121 QEAYETLSDPRRRALYDRDMVGGLQVAFSARRRYDVDEGVPDKSAWKSCWKAQISELKRR 180

Query: 181 SMDKDVRVNSSWGARMRRQMNEQV 199
           SMDKD + N SWGARMR+QMNEQV
Sbjct: 181 SMDKDSKHNLSWGARMRQQMNEQV 204

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BLAST of Moc05g02110 vs. NCBI nr
Match: XP_038893222.1 (chaperone protein dnaJ 20, chloroplastic-like [Benincasa hispida])

HSP 1 Score: 314.3 bits (804), Expect = 7.4e-82
Identity = 165/204 (80.88%), Postives = 174/204 (85.29%), Query Frame = 0


Query: 1   MQCSDFTLSGSESRFYIPSNPSITGRRSFSGHRSRIFFHS-----STRFPSLSIKAKASF 60
           MQCSDFT SGS+SRFYIPSNP+ITGRR    HRSR+FF S     STR P LSI+AKASF
Sbjct: 1   MQCSDFTFSGSDSRFYIPSNPNITGRRPIPCHRSRVFFPSTPPCNSTRSPPLSIRAKASF 60

Query: 61  NEGIVSG-VADGSFYDLLGISQSGSLTEIKRAYKQLARKYHPDVSPPGRAEEYTKRFIRV 120
           NEG VS  VA+GSFYDLLGISQSGSL EIK AYKQLARKYHPDVSPPGRAEEYTKRFIRV
Sbjct: 61  NEGTVSSEVAEGSFYDLLGISQSGSLEEIKHAYKQLARKYHPDVSPPGRAEEYTKRFIRV 120

Query: 121 QEAYETLSDPRRRALYDRDMVRGLQVAFSARRRYEADEGVPERSGWKYCWQAQLSELKRR 180
           QEAYETLSDPRRRALYDRDMV GLQVAFS RRRY+A E VP R  W+  WQAQ+SELKRR
Sbjct: 121 QEAYETLSDPRRRALYDRDMVGGLQVAFSGRRRYDAHEEVPGRREWRNGWQAQISELKRR 180

Query: 181 SMDKDVRVNSSWGARMRRQMNEQV 199
           SMD+D R N SWGARMRRQMNEQV
Sbjct: 181 SMDRDSRQNMSWGARMRRQMNEQV 204

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BLAST of Moc05g02110 vs. NCBI nr
Match: KAG6597272.1 (Chaperone protein dnaJ 20, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 314.3 bits (804), Expect = 7.4e-82
Identity = 162/204 (79.41%), Postives = 175/204 (85.78%), Query Frame = 0


Query: 1   MQCSDFTLSGSESRFYIPSNPSITGRRSFSGHRSRIFFH-----SSTRFPSLSIKAKASF 60
           MQCSDFT S  + R  IPS+PSITGRR  SG RSR+FF      +STR PSLSI+AKASF
Sbjct: 1   MQCSDFTYSVGDYRILIPSSPSITGRRQISGQRSRVFFPNTPPCNSTRLPSLSIRAKASF 60

Query: 61  NEGIVSG-VADGSFYDLLGISQSGSLTEIKRAYKQLARKYHPDVSPPGRAEEYTKRFIRV 120
           N GI S   ADGSFYDLLGIS+SGSL EIKRAYKQLARKYHPDVSPPGRAEEYTKRFIRV
Sbjct: 61  NGGIASSEAADGSFYDLLGISESGSLAEIKRAYKQLARKYHPDVSPPGRAEEYTKRFIRV 120

Query: 121 QEAYETLSDPRRRALYDRDMVRGLQVAFSARRRYEADEGVPERSGWKYCWQAQLSELKRR 180
           QEAYETLSDPRRRALYDRDMV GLQVAFSARRRY+ DEGVP++S WK CW+AQ+SELKRR
Sbjct: 121 QEAYETLSDPRRRALYDRDMVGGLQVAFSARRRYDVDEGVPDKSAWKSCWKAQISELKRR 180

Query: 181 SMDKDVRVNSSWGARMRRQMNEQV 199
           SMDKD + N SWGARMR+QMNEQV
Sbjct: 181 SMDKDSKHNLSWGARMRQQMNEQV 204

[Close]

BLAST of Moc05g02110 vs. NCBI nr
Match: KAG7028743.1 (Chaperone protein dnaJ 20, chloroplastic [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 313.2 bits (801), Expect = 1.7e-81
Identity = 161/204 (78.92%), Postives = 175/204 (85.78%), Query Frame = 0


Query: 1   MQCSDFTLSGSESRFYIPSNPSITGRRSFSGHRSRIFFH-----SSTRFPSLSIKAKASF 60
           MQCSDFT S  + R  IPS+PSITGRR  SG RSR+FF      +STR PSLSI+AKASF
Sbjct: 1   MQCSDFTYSVGDYRILIPSSPSITGRRQISGQRSRVFFPNTPPCNSTRLPSLSIRAKASF 60

Query: 61  NEGIVSG-VADGSFYDLLGISQSGSLTEIKRAYKQLARKYHPDVSPPGRAEEYTKRFIRV 120
           N GI S   ADGSFYDLLGIS+SGSL EIKRAYKQLARKYHPDVSPPGRAEEYTKRFIRV
Sbjct: 61  NGGIASSEAADGSFYDLLGISESGSLAEIKRAYKQLARKYHPDVSPPGRAEEYTKRFIRV 120

Query: 121 QEAYETLSDPRRRALYDRDMVRGLQVAFSARRRYEADEGVPERSGWKYCWQAQLSELKRR 180
           QEAYETLSDPRRRALYDRDMV GLQVAFSARRRY+ DEGVP++S WK CW+AQ+SELK+R
Sbjct: 121 QEAYETLSDPRRRALYDRDMVGGLQVAFSARRRYDVDEGVPDKSAWKSCWKAQISELKKR 180

Query: 181 SMDKDVRVNSSWGARMRRQMNEQV 199
           SMDKD + N SWGARMR+QMNEQV
Sbjct: 181 SMDKDSKHNLSWGARMRQQMNEQV 204

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BLAST of Moc05g02110 vs. ExPASy Swiss-Prot
Match: Q9SDN0 (Chaperone protein dnaJ 20, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=ATJ20 PE=1 SV=2)

HSP 1 Score: 172.2 bits (435), Expect = 6.0e-42
Identity = 95/162 (58.64%), Postives = 114/162 (70.37%), Query Frame = 0


Query: 36  IFFHSSTRFPSLSIKAKASFNEGIVSGVADGSFYDLLGISQSGSLTEIKRAYKQLARKYH 95
           I + + TRF S  I+++ + ++ +     D SFYDLLG+++S +L EIK+AYKQLARKYH
Sbjct: 36  ISYPTRTRFSSTRIQSRLTHDDPVKQS-EDLSFYDLLGVTESVTLPEIKQAYKQLARKYH 95

Query: 96  PDVSPPGRAEEYTKRFIRVQEAYETLSDPRRRALYDRDMVRGLQVAFSARRRYEAD-EGV 155
           PDVSPP R EEYT RFIRVQEAYETLSDPRRR LYDRD+  G   +FS RR+   D E V
Sbjct: 96  PDVSPPDRVEEYTDRFIRVQEAYETLSDPRRRVLYDRDLSMGFSFSFSGRRQNRYDQEVV 155

Query: 156 PERSGWKYCWQAQLSELKRRSMDKDVRVNSSWGARMRRQMNE 197
            E+S WK  WQ QLS L+RRS  KD     SW ARMRRQ  E
Sbjct: 156 EEKSEWKAKWQTQLSGLRRRSNQKD-NNTMSWAARMRRQQQE 195

[Close]

BLAST of Moc05g02110 vs. ExPASy Swiss-Prot
Match: Q8DKR7 (Chaperone protein DnaJ OS=Thermosynechococcus elongatus (strain BP-1) OX=197221 GN=dnaJ PE=3 SV=2)

HSP 1 Score: 74.3 bits (181), Expect = 1.7e-12
Identity = 36/65 (55.38%), Postives = 47/65 (72.31%), Query Frame = 0


Query: 68  FYDLLGISQSGSLTEIKRAYKQLARKYHPDVSPPGRAEEYTKRFIRVQEAYETLSDPRRR 127
           FY++LG+S+S    E+KRAY++LARKYHPDV+    AEE   +F  +  AYE LSDP+ R
Sbjct: 5   FYEILGVSRSADAEELKRAYRRLARKYHPDVNKEPGAEE---KFKEINRAYEVLSDPQAR 64

Query: 128 ALYDR 133
           A YDR
Sbjct: 65  ANYDR 66

[Close]

BLAST of Moc05g02110 vs. ExPASy Swiss-Prot
Match: A5VJE8 (Chaperone protein DnaJ OS=Lactobacillus reuteri (strain DSM 20016) OX=557436 GN=dnaJ PE=3 SV=1)

HSP 1 Score: 73.9 bits (180), Expect = 2.2e-12
Identity = 36/70 (51.43%), Postives = 52/70 (74.29%), Query Frame = 0


Query: 63  VADGSFYDLLGISQSGSLTEIKRAYKQLARKYHPDVSPPGRAEEYTKRFIRVQEAYETLS 122
           +A+  +YD+LG+S+  S  +IKRAY++LA KYHPDV+    AEE   +F ++ EAYETLS
Sbjct: 1   MAEQDYYDILGVSKDASEKDIKRAYRRLAAKYHPDVNHEPGAEE---KFKKINEAYETLS 60

Query: 123 DPRRRALYDR 133
           D ++RA YD+
Sbjct: 61  DSQKRAQYDQ 67

[Close]

BLAST of Moc05g02110 vs. ExPASy Swiss-Prot
Match: B2G6W4 (Chaperone protein DnaJ OS=Lactobacillus reuteri (strain JCM 1112) OX=557433 GN=dnaJ PE=3 SV=1)

HSP 1 Score: 73.9 bits (180), Expect = 2.2e-12
Identity = 36/70 (51.43%), Postives = 52/70 (74.29%), Query Frame = 0


Query: 63  VADGSFYDLLGISQSGSLTEIKRAYKQLARKYHPDVSPPGRAEEYTKRFIRVQEAYETLS 122
           +A+  +YD+LG+S+  S  +IKRAY++LA KYHPDV+    AEE   +F ++ EAYETLS
Sbjct: 1   MAEQDYYDILGVSKDASEKDIKRAYRRLAAKYHPDVNHEPGAEE---KFKKINEAYETLS 60

Query: 123 DPRRRALYDR 133
           D ++RA YD+
Sbjct: 61  DSQKRAQYDQ 67

[Close]

BLAST of Moc05g02110 vs. ExPASy Swiss-Prot
Match: Q3K3T1 (Chaperone protein DnaJ OS=Streptococcus agalactiae serotype Ia (strain ATCC 27591 / A909 / CDC SS700) OX=205921 GN=dnaJ PE=3 SV=1)

HSP 1 Score: 73.9 bits (180), Expect = 2.2e-12
Identity = 36/75 (48.00%), Postives = 51/75 (68.00%), Query Frame = 0


Query: 68  FYDLLGISQSGSLTEIKRAYKQLARKYHPDVSPPGRAEEYTKRFIRVQEAYETLSDPRRR 127
           FYD LG+S+  S  EIK+AY+++++KYHPD++    AEE   ++  VQEAYETLSD ++R
Sbjct: 6   FYDRLGVSKDASQDEIKKAYRRMSKKYHPDINKEAGAEE---KYKEVQEAYETLSDTQKR 65

Query: 128 ALYDRDMVRGLQVAF 143
           A YD+    G    F
Sbjct: 66  AAYDQYGAAGANGGF 77

[Close]

BLAST of Moc05g02110 vs. ExPASy TrEMBL
Match: A0A1S3AWS2 (chaperone protein dnaJ 20, chloroplastic-like OS=Cucumis melo OX=3656 GN=LOC103483465 PE=4 SV=1)

HSP 1 Score: 317.4 bits (812), Expect = 4.3e-83
Identity = 162/204 (79.41%), Postives = 179/204 (87.75%), Query Frame = 0


Query: 1   MQCSDFTLSGSESRFYIPSNPSITGRRSFSGHRSRIFF-----HSSTRFPSLSIKAKASF 60
           MQCSDFT SGS+SRFYIPSNP+I+ RR  SG+RSR+FF      +STR PSLSI+AKASF
Sbjct: 27  MQCSDFTFSGSDSRFYIPSNPTISARRPISGYRSRVFFPHTPPFNSTRLPSLSIRAKASF 86

Query: 61  NEGIVSG-VADGSFYDLLGISQSGSLTEIKRAYKQLARKYHPDVSPPGRAEEYTKRFIRV 120
           NEG VS  VA+GSFYDLLGISQSGSL EIKRAYKQLARKYHPDVSPPG AEEYTK FIRV
Sbjct: 87  NEGFVSSEVAEGSFYDLLGISQSGSLAEIKRAYKQLARKYHPDVSPPGGAEEYTKMFIRV 146

Query: 121 QEAYETLSDPRRRALYDRDMVRGLQVAFSARRRYEADEGVPERSGWKYCWQAQLSELKRR 180
           QEAYETLSDPRRRALYDRDM+ GLQVAFSARRRY+A E V E+SGW+  W+AQ+SELKRR
Sbjct: 147 QEAYETLSDPRRRALYDRDMIGGLQVAFSARRRYDAAEEVAEKSGWRNSWEAQISELKRR 206

Query: 181 SMDKDVRVNSSWGARMRRQMNEQV 199
           SM+KD+R N SWGARMRRQMNEQ+
Sbjct: 207 SMEKDLRQNMSWGARMRRQMNEQI 230

[Close]

BLAST of Moc05g02110 vs. ExPASy TrEMBL
Match: A0A6J1IB32 (chaperone protein dnaJ 20, chloroplastic-like OS=Cucurbita maxima OX=3661 GN=LOC111473471 PE=4 SV=1)

HSP 1 Score: 315.8 bits (808), Expect = 1.2e-82
Identity = 162/204 (79.41%), Postives = 176/204 (86.27%), Query Frame = 0


Query: 1   MQCSDFTLSGSESRFYIPSNPSITGRRSFSGHRSRIFFH-----SSTRFPSLSIKAKASF 60
           MQCSDFT SG + R  IPS+PSITGRR  SG +SR+FF      +STR PSLSI+AKASF
Sbjct: 1   MQCSDFTYSGGDYRILIPSSPSITGRRQISGQKSRVFFPNTPPCNSTRLPSLSIRAKASF 60

Query: 61  NEGIVS-GVADGSFYDLLGISQSGSLTEIKRAYKQLARKYHPDVSPPGRAEEYTKRFIRV 120
           N GI S   ADGSFYDLLGIS+SGSL EIKRAYKQLARKYHPDVSPPGRAEEYTKRFIRV
Sbjct: 61  NGGIASFEAADGSFYDLLGISESGSLAEIKRAYKQLARKYHPDVSPPGRAEEYTKRFIRV 120

Query: 121 QEAYETLSDPRRRALYDRDMVRGLQVAFSARRRYEADEGVPERSGWKYCWQAQLSELKRR 180
           QEAYETLSDPRRRALYDRDMV GLQVAFSARRRY+ DEGVP++S WK CW+AQ+SELKRR
Sbjct: 121 QEAYETLSDPRRRALYDRDMVGGLQVAFSARRRYDVDEGVPDKSAWKSCWKAQISELKRR 180

Query: 181 SMDKDVRVNSSWGARMRRQMNEQV 199
           SMDKD + N SWGARMR+QMNEQV
Sbjct: 181 SMDKDSKHNLSWGARMRQQMNEQV 204

[Close]

BLAST of Moc05g02110 vs. ExPASy TrEMBL
Match: A0A6J1GCT3 (chaperone protein dnaJ 20, chloroplastic-like OS=Cucurbita moschata OX=3662 GN=LOC111452997 PE=4 SV=1)

HSP 1 Score: 313.2 bits (801), Expect = 8.0e-82
Identity = 161/204 (78.92%), Postives = 175/204 (85.78%), Query Frame = 0


Query: 1   MQCSDFTLSGSESRFYIPSNPSITGRRSFSGHRSRIFFH-----SSTRFPSLSIKAKASF 60
           MQCSDFT S  + R  IPS+PSITGRR  SG RSR+FF      +STR PSLSI+AKASF
Sbjct: 35  MQCSDFTYSVGDYRILIPSSPSITGRRQISGQRSRVFFPNTPPCNSTRLPSLSIRAKASF 94

Query: 61  NEGIVSG-VADGSFYDLLGISQSGSLTEIKRAYKQLARKYHPDVSPPGRAEEYTKRFIRV 120
           N GI S   ADGSFYDLLGIS+SGSL EIKRAYKQLARKYHPDVSPPGRAEEYTKRFIRV
Sbjct: 95  NGGIASSEAADGSFYDLLGISESGSLAEIKRAYKQLARKYHPDVSPPGRAEEYTKRFIRV 154

Query: 121 QEAYETLSDPRRRALYDRDMVRGLQVAFSARRRYEADEGVPERSGWKYCWQAQLSELKRR 180
           QEAYETLSDPRRRALYDRDMV GLQVAFSARRRY+ DEGVP++S WK CW+AQ+SELK+R
Sbjct: 155 QEAYETLSDPRRRALYDRDMVGGLQVAFSARRRYDVDEGVPDKSAWKSCWKAQISELKKR 214

Query: 181 SMDKDVRVNSSWGARMRRQMNEQV 199
           SMDKD + N SWGARMR+QMNEQV
Sbjct: 215 SMDKDSKHNLSWGARMRQQMNEQV 238

[Close]

BLAST of Moc05g02110 vs. ExPASy TrEMBL
Match: A0A0A0L4L9 (Chaperone protein dnaJ 20, chloroplast OS=Cucumis sativus OX=3659 GN=Csa_3G129700 PE=4 SV=1)

HSP 1 Score: 306.2 bits (783), Expect = 9.8e-80
Identity = 157/203 (77.34%), Postives = 175/203 (86.21%), Query Frame = 0


Query: 1   MQCSDFTLSGSESRFYIPSNPSITGRRSFSGHRSRIFF-----HSSTRFPSLSIKAKASF 60
           MQCSD T SGS+SRFYIPSNP+ T RR  SG+RSR+ F      +STR PSLSI+AKASF
Sbjct: 14  MQCSDLTFSGSDSRFYIPSNPTTTARRPISGYRSRVCFPHTPPFNSTRLPSLSIRAKASF 73

Query: 61  NEGIVSG-VADGSFYDLLGISQSGSLTEIKRAYKQLARKYHPDVSPPGRAEEYTKRFIRV 120
           NEG VS  VA+GSFYDLLGIS+SGSL EIKRAYKQLARKYHPDVSPPG  EE TKRFIRV
Sbjct: 74  NEGFVSSEVAEGSFYDLLGISKSGSLEEIKRAYKQLARKYHPDVSPPGCVEENTKRFIRV 133

Query: 121 QEAYETLSDPRRRALYDRDMVRGLQVAFSARRRYEADEGVPERSGWKYCWQAQLSELKRR 180
           QEAYETL+DPRRRALYDRDM+ GLQVAFSARRRY+ADE V ++SGW+  W+AQ+SELKRR
Sbjct: 134 QEAYETLADPRRRALYDRDMIGGLQVAFSARRRYDADEEVAQKSGWRNSWEAQISELKRR 193

Query: 181 SMDKDVRVNSSWGARMRRQMNEQ 198
           SM+KD+R N SWGARMRRQMNEQ
Sbjct: 194 SMEKDLRPNMSWGARMRRQMNEQ 216

[Close]

BLAST of Moc05g02110 vs. ExPASy TrEMBL
Match: A0A6J1IRB6 (chaperone protein dnaJ 20, chloroplastic-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111479792 PE=4 SV=1)

HSP 1 Score: 305.4 bits (781), Expect = 1.7e-79
Identity = 158/204 (77.45%), Postives = 174/204 (85.29%), Query Frame = 0


Query: 1   MQCSDFTLSGSESRFYIPSNPSITGRRSFSGHRSRIFFHSS-----TRFPSLSIKAKASF 60
           MQCSDFT SGS+SRFYIPS+PSITGRR  +GHRS IFF ++     TR PSLSI+AKASF
Sbjct: 1   MQCSDFTFSGSDSRFYIPSSPSITGRRPITGHRSCIFFPNTLPCNLTRLPSLSIRAKASF 60

Query: 61  NEGIVSG-VADGSFYDLLGISQSGSLTEIKRAYKQLARKYHPDVSPPGRAEEYTKRFIRV 120
           NEGIVS  V+DGSFYDLLG+S+S SL EIKRAYK LARKYHPDVSP G+AEEYTKRFIRV
Sbjct: 61  NEGIVSSEVSDGSFYDLLGVSESVSLAEIKRAYKHLARKYHPDVSPIGQAEEYTKRFIRV 120

Query: 121 QEAYETLSDPRRRALYDRDMVRGLQVAFSARRRYEADEGVPERSGWKYCWQAQLSELKRR 180
            EAYETLSDPRRRALYDRDMV GLQVAFSA R Y+AD+  PE+SGW+ CWQ Q+SEL RR
Sbjct: 121 HEAYETLSDPRRRALYDRDMVGGLQVAFSAWRLYDADQEAPEKSGWRSCWQFQISELNRR 180

Query: 181 SMDKDVRVNSSWGARMRRQMNEQV 199
           SM+KD   N SWGARMRRQMNEQV
Sbjct: 181 SMNKDSSQNMSWGARMRRQMNEQV 204

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BLAST of Moc05g02110 vs. TAIR 10
Match: AT4G13830.2 (DNAJ-like 20 )

HSP 1 Score: 172.2 bits (435), Expect = 4.3e-43
Identity = 95/162 (58.64%), Postives = 114/162 (70.37%), Query Frame = 0


Query: 36  IFFHSSTRFPSLSIKAKASFNEGIVSGVADGSFYDLLGISQSGSLTEIKRAYKQLARKYH 95
           I + + TRF S  I+++ + ++ +     D SFYDLLG+++S +L EIK+AYKQLARKYH
Sbjct: 36  ISYPTRTRFSSTRIQSRLTHDDPVKQS-EDLSFYDLLGVTESVTLPEIKQAYKQLARKYH 95

Query: 96  PDVSPPGRAEEYTKRFIRVQEAYETLSDPRRRALYDRDMVRGLQVAFSARRRYEAD-EGV 155
           PDVSPP R EEYT RFIRVQEAYETLSDPRRR LYDRD+  G   +FS RR+   D E V
Sbjct: 96  PDVSPPDRVEEYTDRFIRVQEAYETLSDPRRRVLYDRDLSMGFSFSFSGRRQNRYDQEVV 155

Query: 156 PERSGWKYCWQAQLSELKRRSMDKDVRVNSSWGARMRRQMNE 197
            E+S WK  WQ QLS L+RRS  KD     SW ARMRRQ  E
Sbjct: 156 EEKSEWKAKWQTQLSGLRRRSNQKD-NNTMSWAARMRRQQQE 195

[Close]

BLAST of Moc05g02110 vs. TAIR 10
Match: AT4G13830.1 (DNAJ-like 20 )

HSP 1 Score: 135.6 bits (340), Expect = 4.4e-32
Identity = 69/117 (58.97%), Postives = 87/117 (74.36%), Query Frame = 0


Query: 36  IFFHSSTRFPSLSIKAKASFNEGIVSGVADGSFYDLLGISQSGSLTEIKRAYKQLARKYH 95
           I + + TRF S  I+++ + ++ +     D SFYDLLG+++S +L EIK+AYKQLARKYH
Sbjct: 36  ISYPTRTRFSSTRIQSRLTHDDPVKQS-EDLSFYDLLGVTESVTLPEIKQAYKQLARKYH 95

Query: 96  PDVSPPGRAEEYTKRFIRVQEAYETLSDPRRRALYDRDMVRGLQVAFSARRRYEADE 153
           PDVSPP R EEYT RFIRVQEAYETLSDPRRR LYDRD+  G   +FS RR+   D+
Sbjct: 96  PDVSPPDRVEEYTDRFIRVQEAYETLSDPRRRVLYDRDLSMGFSFSFSGRRQNRYDQ 151

[Close]

BLAST of Moc05g02110 vs. TAIR 10
Match: AT2G22360.1 (DNAJ heat shock family protein )

HSP 1 Score: 70.5 bits (171), Expect = 1.7e-12
Identity = 50/138 (36.23%), Postives = 73/138 (52.90%), Query Frame = 0


Query: 14  RFYIPSNPSITGRRSFSGH-------RSRIFFHSSTRFPSLSIKAKASF--NEGIVSGV- 73
           R Y PS    T  ++ S         +S +F H S  F S++  ++       G    V 
Sbjct: 23  RAYYPSRIESTRHQNSSSQVNCLGASKSSMFSHGSLPFLSMTGMSRNMHPPRRGSRFTVR 82

Query: 74  ADGSFYDLLGISQSGSLTEIKRAYKQLARKYHPDVSPPGRAEEYTKRFIRVQEAYETLSD 133
           AD  +Y +LG+S++ +  EIK AY++LAR YHPDV+    AEE   +F  +  AYE LSD
Sbjct: 83  ADADYYSVLGVSKNATKAEIKSAYRKLARNYHPDVNKDPGAEE---KFKEISNAYEVLSD 142

Query: 134 PRRRALYDRDMVRGLQVA 142
             +++LYDR    GL+ A
Sbjct: 143 DEKKSLYDRYGEAGLKGA 157

[Close]

BLAST of Moc05g02110 vs. TAIR 10
Match: AT4G39960.1 (Molecular chaperone Hsp40/DnaJ family protein )

HSP 1 Score: 70.5 bits (171), Expect = 1.7e-12
Identity = 43/102 (42.16%), Postives = 59/102 (57.84%), Query Frame = 0


Query: 33  RSRIFFHSSTRFPSLS-IKAKASFNEGIVSGV-ADGSFYDLLGISQSGSLTEIKRAYKQL 92
           RS +F   S  F SL+ +        G    V AD  FY +LG+S++ +  EIK AY++L
Sbjct: 49  RSSMFAQGSLPFLSLTGVSPNTHSRRGARFTVRADTDFYSVLGVSKNATKAEIKSAYRKL 108

Query: 93  ARKYHPDVSPPGRAEEYTKRFIRVQEAYETLSDPRRRALYDR 133
           AR YHPDV+    AE+   +F  +  AYE LSD  +R+LYDR
Sbjct: 109 ARSYHPDVNKDAGAED---KFKEISNAYEILSDDEKRSLYDR 147

[Close]

BLAST of Moc05g02110 vs. TAIR 10
Match: AT1G80030.1 (Molecular chaperone Hsp40/DnaJ family protein )

HSP 1 Score: 68.6 bits (166), Expect = 6.6e-12
Identity = 42/108 (38.89%), Postives = 62/108 (57.41%), Query Frame = 0


Query: 25  GRRSFSGHRSRIFFHSSTRFPSLSIKAKASFNEGIVSGVADGSFYDLLGISQSGSLTEIK 84
           G  +F  H+S +   SS+   S S   +      +V     G +Y  LG+S+S +  EIK
Sbjct: 36  GGSNFRYHKSFLSLSSSS---SSSTPYRNRRGRSLVVFATSGDYYATLGVSKSANNKEIK 95

Query: 85  RAYKQLARKYHPDVSPPGRAEEYTKRFIRVQEAYETLSDPRRRALYDR 133
            AY++LAR+YHPDV+    A   T++F  +  AYE LSD ++RALYD+
Sbjct: 96  AAYRRLARQYHPDVNKEPGA---TEKFKEISAAYEVLSDEQKRALYDQ 137

The following BLAST results are available for this feature:

BLAST of Moc05g02110 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_008438327.1	8.8e-83	79.41	PREDICTED: chaperone protein dnaJ 20, chloroplastic-like [Cucumis melo]	[more]
XP_022974737.1	2.6e-82	79.41	chaperone protein dnaJ 20, chloroplastic-like [Cucurbita maxima]	[more]
XP_038893222.1	7.4e-82	80.88	chaperone protein dnaJ 20, chloroplastic-like [Benincasa hispida]	[more]
KAG6597272.1	7.4e-82	79.41	Chaperone protein dnaJ 20, chloroplastic, partial [Cucurbita argyrosperma subsp....	[more]
KAG7028743.1	1.7e-81	78.92	Chaperone protein dnaJ 20, chloroplastic [Cucurbita argyrosperma subsp. argyrosp...	[more]

BLAST of Moc05g02110 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9SDN0	6.0e-42	58.64	Chaperone protein dnaJ 20, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=ATJ2...	[more]
Q8DKR7	1.7e-12	55.38	Chaperone protein DnaJ OS=Thermosynechococcus elongatus (strain BP-1) OX=197221 ...	[more]
A5VJE8	2.2e-12	51.43	Chaperone protein DnaJ OS=Lactobacillus reuteri (strain DSM 20016) OX=557436 GN=...	[more]
B2G6W4	2.2e-12	51.43	Chaperone protein DnaJ OS=Lactobacillus reuteri (strain JCM 1112) OX=557433 GN=d...	[more]
Q3K3T1	2.2e-12	48.00	Chaperone protein DnaJ OS=Streptococcus agalactiae serotype Ia (strain ATCC 2759...	[more]

BLAST of Moc05g02110 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A1S3AWS2	4.3e-83	79.41	chaperone protein dnaJ 20, chloroplastic-like OS=Cucumis melo OX=3656 GN=LOC1034...	[more]
A0A6J1IB32	1.2e-82	79.41	chaperone protein dnaJ 20, chloroplastic-like OS=Cucurbita maxima OX=3661 GN=LOC...	[more]
A0A6J1GCT3	8.0e-82	78.92	chaperone protein dnaJ 20, chloroplastic-like OS=Cucurbita moschata OX=3662 GN=L...	[more]
A0A0A0L4L9	9.8e-80	77.34	Chaperone protein dnaJ 20, chloroplast OS=Cucumis sativus OX=3659 GN=Csa_3G12970...	[more]
A0A6J1IRB6	1.7e-79	77.45	chaperone protein dnaJ 20, chloroplastic-like isoform X1 OS=Cucurbita maxima OX=...	[more]

BLAST of Moc05g02110 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G13830.2	4.3e-43	58.64	DNAJ-like 20	[more]
AT4G13830.1	4.4e-32	58.97	DNAJ-like 20	[more]
AT2G22360.1	1.7e-12	36.23	DNAJ heat shock family protein	[more]
AT4G39960.1	1.7e-12	42.16	Molecular chaperone Hsp40/DnaJ family protein	[more]
AT1G80030.1	6.6e-12	38.89	Molecular chaperone Hsp40/DnaJ family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (OHB3-1) v2
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001623	DnaJ domain	PRINTS	PR00625	JDOMAIN	coord: 106..126 score: 39.13 coord: 87..102 score: 48.68 coord: 126..145 score: 23.59 coord: 69..87 score: 44.89
IPR001623	DnaJ domain	SMART	SM00271	dnaj_3	coord: 66..126 e-value: 1.1E-20 score: 84.8
IPR001623	DnaJ domain	PFAM	PF00226	DnaJ	coord: 68..131 e-value: 6.4E-21 score: 74.3
IPR001623	DnaJ domain	PROSITE	PS50076	DNAJ_2	coord: 67..134 score: 20.098667
IPR001623	DnaJ domain	CDD	cd06257	DnaJ	coord: 68..123 e-value: 1.02955E-19 score: 76.8153
IPR036869	Chaperone J-domain superfamily	GENE3D	1.10.287.110	DnaJ domain	coord: 62..152 e-value: 1.3E-22 score: 81.8
IPR036869	Chaperone J-domain superfamily	SUPERFAMILY	46565	Chaperone J-domain	coord: 67..143
None	No IPR available	PANTHER	PTHR45090	CHAPERONE PROTEIN DNAJ 20 CHLOROPLASTIC	coord: 1..197
None	No IPR available	PANTHER	PTHR45090:SF4	CHAPERONE PROTEIN DNAJ 20, CHLOROPLASTIC	coord: 1..197
IPR018253	DnaJ domain, conserved site	PROSITE	PS00636	DNAJ_1	coord: 111..130

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Moc05g02110.1	Moc05g02110.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0061077	chaperone-mediated protein folding
cellular_component	GO:0009507	chloroplast