Moc05g00930 (gene) Bitter gourd (OHB3-1) v2

Overview
NameMoc05g00930
Typegene
OrganismMomordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
DescriptionActin-depolymerizing factor
Locationchr5: 624187 .. 625956 (-)
RNA-Seq ExpressionMoc05g00930
SyntenyMoc05g00930
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGCCTCCCCCTAAGGTACATTCTAAGGCAAACTTGACTTGGCAGGCATGAACGTCGAAAGTGGCGAGGAGTCACTCGCCAACGTAGAGTGCACGTTTGAACTCCGATTGACACGAAACTTGGCGTGCTTACATATCTCACTAATATGCATCTAACGTTAAAGCCGCTCGTCCAAACTCTTGACAGAACCCCAACTTCTAGGGTCGTGGCCCGGGGCCCCAACTGGCCCCCGTTGAGCTTGTCACCGACACCACTGACGTCGCCTTCGCTTTGCATTCTGCATATGCCTTCTCTAGGCATTCTTGAGCACATCCATATGGTGTGATAGGTGATGCACCATCCTCCTTGGTCGCATCACGGCACTTCACTCTCGGCCCTTGACATGGCCCGATGGGCGACATATGCTTGTCGCTGACACTTGCACACTACGTGGGCGTCACAACCTACTCTCTACTAGTATGGTTGTGACACTGAGCATAGCAAAAGCACATACCTGGAACTTCAGCGAAAGAAGGTTCACCGGTATGTGATTTTTATGATTGATGAAAAGAAAAAAGAGGTGGTGGTGGAAAAGACTGGGAGCCCAGCTGAGAGCTTTGATGATTTCACTGCTTCGTTGCCTGAGAATGACTATAGATATGCAGTTTTTGACTTTGATTTTGTGACTTCTGAGAACTACCAAAAGAGCAAAATCTTTTTCATTTCATGGTTAGTTATTTTAATTCATTTTAAAAGTATTTCCCGGGTTAGTTCAGTAATTTGACTTATTGTTTAGTTTTCTTCTTGTTCCTTTTATGTTTGTTATCAGGTTCAACCTCCAACTATTGGTACCTCTAGTATTAAAGGAGTATTGCATTTACTTCTTGGAACTTTCATTACTGTATCTGCAAGATTCTGCTTACTTTCATGTTCTTTAACCATCTCATTATATCAAGTATTTTTCTTTGTGTTAAACCTAAAGATCAGGTGATATTTCTAGCTTAGCTGGCATGAATTAAAAACACAAATGCCTTGTGGTATGGATGAACGGTGAAAATTTTGGGGAGCCGTCAAATACCTAACGTATTTGATCAACTTTCTTTCCGATATTGTTTCATGAAATTATTGTCTTGTTGTATAGTTAAGCGTTATATATGTCACTCTGCCTTTAACAACAGGGATTTAGGAAATTTATATACGAGGGTGGTTTTCTACTTATATTATCTGAGATTAGTAATTCCACTTGGTTGTTAAATTGCTCCATGAGTTTGCCCTGTTGTTGTAATAACATAATCAGTTGTTTTAGTTCCCGCCCTCTTGCTATTGCTTTGCTGTTGTACCAAGAAACCGTTCATTCATAACTATTCTTATTGTAATATTTTTGACCTGCAGTCTGGTTCTACAAGGCATTGGCAATTACCTGCCAATCTTTGACACTCTTTCCTCTAGGGGGACGCATCCAGTCTGTAGTTCCATACCTGATTTCTTACATTTTCTGTACACTAGCAGACATATTCAATGTTTTGATACTGTTATTATAGTTGTGCCGGCGTAGAAATAAAGTGGTGCGGTGGTTATGCTGACTGAGTGACTGTTTGATGCACTCTTGCTCAAGATGATCTACTCCAGCTCTTGTGATTTTTTGACGATTACTATCTTTGCAGGTCTCCATCTGTATCAAGGATCCGTTCCAAGATGCTTTATGCAACATCAAAGGACAGGTTTAGAAGGGAATTGGAGGGAATCCATTACGAGATTCAGGCAACTGACCCAACTGAAGATGGATATTGA

mRNA sequence

ATGCCTCCCCCTAAGCCGCTCGTCCAAACTCTTGACAGAACCCCAACTTCTAGGGTCGTGGCCCGGGGCCCCAACTGGCCCCCGTTGAGCTTGTCACCGACACCACTGACGTCGCCTTCGCTTTGCATTCTGCATATGCCTTCTCTAGGCATTCTTGAGCACATCCATATGCGAAAGAAGGTTCACCGGTATGTGATTTTTATGATTGATGAAAAGAAAAAAGAGGTGGTGGTGGAAAAGACTGGGAGCCCAGCTGAGAGCTTTGATGATTTCACTGCTTCGTTGCCTGAGAATGACTATAGATATGCAGTTTTTGACTTTGATTTTGTGACTTCTGAGAACTACCAAAAGAGCAAAATCTTTTTCATTTCATGGTTCAACCTCCAACTATTGGTACCTCTAGTATTAAAGGAGTATTGCATTTACTTCTTGGAACTTTCATTACTTCTGGTTCTACAAGGCATTGGCAATTACCTGCCAATCTTTGACACTCTTTCCTCTAGGGGGACGCATCCAGTCTGTAGTTCCATACCTGATTTCTTACATTTTCTGTCTCCATCTGTATCAAGGATCCGTTCCAAGATGCTTTATGCAACATCAAAGGACAGGTTTAGAAGGGAATTGGAGGGAATCCATTACGAGATTCAGGCAACTGACCCAACTGAAGATGGATATTGA

Coding sequence (CDS)

ATGCCTCCCCCTAAGCCGCTCGTCCAAACTCTTGACAGAACCCCAACTTCTAGGGTCGTGGCCCGGGGCCCCAACTGGCCCCCGTTGAGCTTGTCACCGACACCACTGACGTCGCCTTCGCTTTGCATTCTGCATATGCCTTCTCTAGGCATTCTTGAGCACATCCATATGCGAAAGAAGGTTCACCGGTATGTGATTTTTATGATTGATGAAAAGAAAAAAGAGGTGGTGGTGGAAAAGACTGGGAGCCCAGCTGAGAGCTTTGATGATTTCACTGCTTCGTTGCCTGAGAATGACTATAGATATGCAGTTTTTGACTTTGATTTTGTGACTTCTGAGAACTACCAAAAGAGCAAAATCTTTTTCATTTCATGGTTCAACCTCCAACTATTGGTACCTCTAGTATTAAAGGAGTATTGCATTTACTTCTTGGAACTTTCATTACTTCTGGTTCTACAAGGCATTGGCAATTACCTGCCAATCTTTGACACTCTTTCCTCTAGGGGGACGCATCCAGTCTGTAGTTCCATACCTGATTTCTTACATTTTCTGTCTCCATCTGTATCAAGGATCCGTTCCAAGATGCTTTATGCAACATCAAAGGACAGGTTTAGAAGGGAATTGGAGGGAATCCATTACGAGATTCAGGCAACTGACCCAACTGAAGATGGATATTGA

Protein sequence

MPPPKPLVQTLDRTPTSRVVARGPNWPPLSLSPTPLTSPSLCILHMPSLGILEHIHMRKKVHRYVIFMIDEKKKEVVVEKTGSPAESFDDFTASLPENDYRYAVFDFDFVTSENYQKSKIFFISWFNLQLLVPLVLKEYCIYFLELSLLLVLQGIGNYLPIFDTLSSRGTHPVCSSIPDFLHFLSPSVSRIRSKMLYATSKDRFRRELEGIHYEIQATDPTEDGY
Homology
BLAST of Moc05g00930 vs. NCBI nr
Match: XP_022146448.1 (actin-depolymerizing factor-like isoform X2 [Momordica charantia])

HSP 1 Score: 193.4 bits (490), Expect = 2.2e-45
Identity = 112/183 (61.20%), Postives = 114/183 (62.30%), Query Frame = 0

Query: 49  LGILEHIH------MRKKVHRYVIFMIDEKKKEVVVEKTGSPAESFDDFTASLPENDYRY 108
           +G+ EH         RKKVHRYVIFMIDEKKKEVVVEKTGSPAESFDDFTASLPENDYRY
Sbjct: 1   MGVAEHSKSTYLELQRKKVHRYVIFMIDEKKKEVVVEKTGSPAESFDDFTASLPENDYRY 60

Query: 109 AVFDFDFVTSENYQKSKIFFISWFNLQLLVPLVLKEYCIYFLELSLLLVLQGIGNYLPIF 168
           AVFDFDFVTSENYQKSKIFFISW                                     
Sbjct: 61  AVFDFDFVTSENYQKSKIFFISW------------------------------------- 120

Query: 169 DTLSSRGTHPVCSSIPDFLHFLSPSVSRIRSKMLYATSKDRFRRELEGIHYEIQATDPTE 226
                                 SPSVSRIRSKMLYATSKDRFRRELEGIHYEIQATDPTE
Sbjct: 121 ----------------------SPSVSRIRSKMLYATSKDRFRRELEGIHYEIQATDPTE 124

BLAST of Moc05g00930 vs. NCBI nr
Match: XP_022146440.1 (actin-depolymerizing factor-like isoform X1 [Momordica charantia])

HSP 1 Score: 193.0 bits (489), Expect = 2.8e-45
Identity = 109/168 (64.88%), Postives = 109/168 (64.88%), Query Frame = 0

Query: 58  RKKVHRYVIFMIDEKKKEVVVEKTGSPAESFDDFTASLPENDYRYAVFDFDFVTSENYQK 117
           RKKVHRYVIFMIDEKKKEVVVEKTGSPAESFDDFTASLPENDYRYAVFDFDFVTSENYQK
Sbjct: 16  RKKVHRYVIFMIDEKKKEVVVEKTGSPAESFDDFTASLPENDYRYAVFDFDFVTSENYQK 75

Query: 118 SKIFFISWFNLQLLVPLVLKEYCIYFLELSLLLVLQGIGNYLPIFDTLSSRGTHPVCSSI 177
           SKIFFISW                                                    
Sbjct: 76  SKIFFISW---------------------------------------------------- 124

Query: 178 PDFLHFLSPSVSRIRSKMLYATSKDRFRRELEGIHYEIQATDPTEDGY 226
                  SPSVSRIRSKMLYATSKDRFRRELEGIHYEIQATDPTEDGY
Sbjct: 136 -------SPSVSRIRSKMLYATSKDRFRRELEGIHYEIQATDPTEDGY 124

BLAST of Moc05g00930 vs. NCBI nr
Match: RVW86811.1 (Actin-depolymerizing factor 11 [Vitis vinifera])

HSP 1 Score: 181.8 bits (460), Expect = 6.6e-42
Identity = 110/183 (60.11%), Postives = 124/183 (67.76%), Query Frame = 0

Query: 49  LGILEHIH------MRKKVHRYVIFMIDEKKKEVVVEKTGSPAESFDDFTASLPENDYRY 108
           +G+ +H         RKKVHRYVIF IDEKKKEVVVEKTG PAES+DDFTASLPEND RY
Sbjct: 55  MGVADHCKATYLELQRKKVHRYVIFKIDEKKKEVVVEKTGGPAESYDDFTASLPENDCRY 114

Query: 109 AVFDFDFVTSENYQKSKIFFISW---FNLQLLVPLVLKEYCIYFLELSLLLVLQGIGNYL 168
           A++DFDFVTSEN QKSKIFFI+W    N QL  P  L   C+  +  S   ++       
Sbjct: 115 AIYDFDFVTSENCQKSKIFFIAWQWSNNFQLFDP-SLSFPCLQEMTFSCSCLISA----Q 174

Query: 169 PIFDTLSSRGTHPVCSSIPDFLHFLSPSVSRIRSKMLYATSKDRFRRELEGIHYEIQATD 223
            I   L+  G             +LSPSVSRIR+KMLYATSKDRFRRELEGIHYEIQATD
Sbjct: 175 LIASVLAGGGV------------YLSPSVSRIRAKMLYATSKDRFRRELEGIHYEIQATD 220

BLAST of Moc05g00930 vs. NCBI nr
Match: RVW33367.1 (Actin-depolymerizing factor 11 [Vitis vinifera])

HSP 1 Score: 181.8 bits (460), Expect = 6.6e-42
Identity = 113/191 (59.16%), Postives = 126/191 (65.97%), Query Frame = 0

Query: 49  LGILEHIH------MRKKVHRYVIFMIDEKKKEVVVEKTGSPAESFDDFTASLPENDYRY 108
           +G+ +H         RKKVHRYVIF IDEKKKEVVVEKTG PAES+DDFTASLPEND RY
Sbjct: 81  MGVADHCKATYLELQRKKVHRYVIFKIDEKKKEVVVEKTGGPAESYDDFTASLPENDCRY 140

Query: 109 AVFDFDFVTSENYQKSKIFFISW---FNLQLLVPLV----LKEY---CIYFLELSLLL-V 168
           A++DFDFVTSEN QKSKIFFI+W    N QL  P +    L+E    C   +   L+  V
Sbjct: 141 AIYDFDFVTSENCQKSKIFFIAWQWSNNFQLFDPSLSFPCLQEMTFSCSCLISAQLIASV 200

Query: 169 LQGIGNYLPIFDTLSSRGTHPVCSSIPDFLHFLSPSVSRIRSKMLYATSKDRFRRELEGI 223
           L G+G Y                         LSPSVSRIR+KMLYATSKDRFRRELEGI
Sbjct: 201 LAGVGVY-------------------------LSPSVSRIRAKMLYATSKDRFRRELEGI 246

BLAST of Moc05g00930 vs. NCBI nr
Match: EXC01786.1 (Actin-depolymerizing factor [Morus notabilis])

HSP 1 Score: 178.7 bits (452), Expect = 5.5e-41
Identity = 105/167 (62.87%), Postives = 118/167 (70.66%), Query Frame = 0

Query: 58  RKKVHRYVIFMIDEKKKEVVVEKTGSPAESFDDFTASLPENDYRYAVFDFDFVTSENYQK 117
           RKKV RYVIF IDEKKKEV+VEKTG PAESFDDF ASLPEND RYAV+DFDFVTSEN QK
Sbjct: 16  RKKVFRYVIFKIDEKKKEVMVEKTGGPAESFDDFAASLPENDCRYAVYDFDFVTSENCQK 75

Query: 118 SKIFFISWFNLQLLVPLVLKEYCIYFLELSLLLVLQGIGNYLPI--FDTLSSRGTHPVCS 177
           SKIFFI+     +  P  L    +YF+ L         G+ LP+  F  L       +  
Sbjct: 76  SKIFFIACKGKDVENPSELSSASLYFMPLD--------GSPLPVYFFPPL-------IFL 135

Query: 178 SIPDFLHFLSPSVSRIRSKMLYATSKDRFRRELEGIHYEIQATDPTE 223
           + P  L   SPSVSRIR+KMLYATSKD+FRREL+GIHYEIQATDPTE
Sbjct: 136 TFPGVLLLRSPSVSRIRAKMLYATSKDKFRRELDGIHYEIQATDPTE 167

BLAST of Moc05g00930 vs. ExPASy Swiss-Prot
Match: Q8SAG3 (Actin-depolymerizing factor OS=Vitis vinifera OX=29760 PE=2 SV=1)

HSP 1 Score: 159.5 bits (402), Expect = 4.6e-38
Identity = 91/165 (55.15%), Postives = 99/165 (60.00%), Query Frame = 0

Query: 58  RKKVHRYVIFMIDEKKKEVVVEKTGSPAESFDDFTASLPENDYRYAVFDFDFVTSENYQK 117
           RKKVHRYVIF IDEKKKEVVVEKTG PAESFD+F A+LPEND RYAV+DFDFVTSEN QK
Sbjct: 27  RKKVHRYVIFKIDEKKKEVVVEKTGGPAESFDEFAAALPENDCRYAVYDFDFVTSENCQK 86

Query: 118 SKIFFISWFNLQLLVPLVLKEYCIYFLELSLLLVLQGIGNYLPIFDTLSSRGTHPVCSSI 177
           SKIFFI+W                                                    
Sbjct: 87  SKIFFIAW---------------------------------------------------- 132

Query: 178 PDFLHFLSPSVSRIRSKMLYATSKDRFRRELEGIHYEIQATDPTE 223
                  SP  SRIR+KMLYATSK+RFRREL+G+HYEIQATDPTE
Sbjct: 147 -------SPDSSRIRAKMLYATSKERFRRELDGVHYEIQATDPTE 132

BLAST of Moc05g00930 vs. ExPASy Swiss-Prot
Match: Q2QLT8 (Actin-depolymerizing factor 11 OS=Oryza sativa subsp. japonica OX=39947 GN=ADF11 PE=2 SV=1)

HSP 1 Score: 147.9 bits (372), Expect = 1.4e-34
Identity = 85/165 (51.52%), Postives = 96/165 (58.18%), Query Frame = 0

Query: 58  RKKVHRYVIFMIDEKKKEVVVEKTGSPAESFDDFTASLPENDYRYAVFDFDFVTSENYQK 117
           RKK HRYVIF ID+K KEVVVEKTGS  ESFDDF  SLPE+D RYA++DFDFVT EN QK
Sbjct: 29  RKKSHRYVIFKIDDKCKEVVVEKTGSSTESFDDFMDSLPESDCRYAIYDFDFVTEENCQK 88

Query: 118 SKIFFISWFNLQLLVPLVLKEYCIYFLELSLLLVLQGIGNYLPIFDTLSSRGTHPVCSSI 177
           SKIFF++W                                                    
Sbjct: 89  SKIFFVAW---------------------------------------------------- 134

Query: 178 PDFLHFLSPSVSRIRSKMLYATSKDRFRRELEGIHYEIQATDPTE 223
                  SPSVSRIR+KMLYATSK+RFRREL+G+HYEIQATDP+E
Sbjct: 149 -------SPSVSRIRAKMLYATSKERFRRELDGVHYEIQATDPSE 134

BLAST of Moc05g00930 vs. ExPASy Swiss-Prot
Match: Q9ZSK2 (Actin-depolymerizing factor 6 OS=Arabidopsis thaliana OX=3702 GN=ADF6 PE=1 SV=1)

HSP 1 Score: 145.6 bits (366), Expect = 6.8e-34
Identity = 83/165 (50.30%), Postives = 94/165 (56.97%), Query Frame = 0

Query: 58  RKKVHRYVIFMIDEKKKEVVVEKTGSPAESFDDFTASLPENDYRYAVFDFDFVTSENYQK 117
           RKK HRYV+F IDE KKEVVVEKTG+P ES+DDF ASLP+ND RYAV+DFDFVTSEN QK
Sbjct: 30  RKKTHRYVVFKIDESKKEVVVEKTGNPTESYDDFLASLPDNDCRYAVYDFDFVTSENCQK 89

Query: 118 SKIFFISWFNLQLLVPLVLKEYCIYFLELSLLLVLQGIGNYLPIFDTLSSRGTHPVCSSI 177
           SKIFF +W                                                    
Sbjct: 90  SKIFFFAW---------------------------------------------------- 135

Query: 178 PDFLHFLSPSVSRIRSKMLYATSKDRFRRELEGIHYEIQATDPTE 223
                  SPS S IR+K+LY+TSKD+  REL+GIHYEIQATDPTE
Sbjct: 150 -------SPSTSGIRAKVLYSTSKDQLSRELQGIHYEIQATDPTE 135

BLAST of Moc05g00930 vs. ExPASy Swiss-Prot
Match: Q9AY76 (Actin-depolymerizing factor 2 OS=Oryza sativa subsp. japonica OX=39947 GN=ADF2 PE=2 SV=1)

HSP 1 Score: 139.4 bits (350), Expect = 4.9e-32
Identity = 80/164 (48.78%), Postives = 93/164 (56.71%), Query Frame = 0

Query: 59  KKVHRYVIFMIDEKKKEVVVEKTGSPAESFDDFTASLPENDYRYAVFDFDFVTSENYQKS 118
           KK  RYVIF I+EK+K+VVVEKTG+  ES+DDF ASLPEND RYA++DFDFVT EN QKS
Sbjct: 30  KKAFRYVIFKIEEKQKQVVVEKTGATTESYDDFLASLPENDCRYALYDFDFVTGENVQKS 89

Query: 119 KIFFISWFNLQLLVPLVLKEYCIYFLELSLLLVLQGIGNYLPIFDTLSSRGTHPVCSSIP 178
           KIFFI+W                                                     
Sbjct: 90  KIFFIAW----------------------------------------------------- 134

Query: 179 DFLHFLSPSVSRIRSKMLYATSKDRFRRELEGIHYEIQATDPTE 223
                 SPS SRIR+KMLY+TSKDR ++EL+G HYEIQATDPTE
Sbjct: 150 ------SPSTSRIRAKMLYSTSKDRIKQELDGFHYEIQATDPTE 134

BLAST of Moc05g00930 vs. ExPASy Swiss-Prot
Match: Q337A5 (Actin-depolymerizing factor 10 OS=Oryza sativa subsp. japonica OX=39947 GN=ADF10 PE=2 SV=1)

HSP 1 Score: 132.9 bits (333), Expect = 4.6e-30
Identity = 73/165 (44.24%), Postives = 95/165 (57.58%), Query Frame = 0

Query: 58  RKKVHRYVIFMIDEKKKEVVVEKTGSPAESFDDFTASLPENDYRYAVFDFDFVTSENYQK 117
           R+KVHRYVIF ID++++E+VVEKTG+P ES+DDFTASLP +D RYAV+D DFV+ +N +K
Sbjct: 33  RRKVHRYVIFKIDDRREEIVVEKTGAPGESYDDFTASLPADDCRYAVYDLDFVSDDNCRK 92

Query: 118 SKIFFISWFNLQLLVPLVLKEYCIYFLELSLLLVLQGIGNYLPIFDTLSSRGTHPVCSSI 177
           SKIFFISW                                                    
Sbjct: 93  SKIFFISW---------------------------------------------------- 138

Query: 178 PDFLHFLSPSVSRIRSKMLYATSKDRFRRELEGIHYEIQATDPTE 223
                  SPSVSRIR+K +YA S+++FR EL+G+H+EIQATDP +
Sbjct: 153 -------SPSVSRIRAKTIYAVSRNQFRHELDGVHFEIQATDPDD 138

BLAST of Moc05g00930 vs. ExPASy TrEMBL
Match: A0A6J1CYL7 (actin-depolymerizing factor-like isoform X2 OS=Momordica charantia OX=3673 GN=LOC111015653 PE=3 SV=1)

HSP 1 Score: 193.4 bits (490), Expect = 1.1e-45
Identity = 112/183 (61.20%), Postives = 114/183 (62.30%), Query Frame = 0

Query: 49  LGILEHIH------MRKKVHRYVIFMIDEKKKEVVVEKTGSPAESFDDFTASLPENDYRY 108
           +G+ EH         RKKVHRYVIFMIDEKKKEVVVEKTGSPAESFDDFTASLPENDYRY
Sbjct: 1   MGVAEHSKSTYLELQRKKVHRYVIFMIDEKKKEVVVEKTGSPAESFDDFTASLPENDYRY 60

Query: 109 AVFDFDFVTSENYQKSKIFFISWFNLQLLVPLVLKEYCIYFLELSLLLVLQGIGNYLPIF 168
           AVFDFDFVTSENYQKSKIFFISW                                     
Sbjct: 61  AVFDFDFVTSENYQKSKIFFISW------------------------------------- 120

Query: 169 DTLSSRGTHPVCSSIPDFLHFLSPSVSRIRSKMLYATSKDRFRRELEGIHYEIQATDPTE 226
                                 SPSVSRIRSKMLYATSKDRFRRELEGIHYEIQATDPTE
Sbjct: 121 ----------------------SPSVSRIRSKMLYATSKDRFRRELEGIHYEIQATDPTE 124

BLAST of Moc05g00930 vs. ExPASy TrEMBL
Match: A0A6J1CX92 (actin-depolymerizing factor-like isoform X1 OS=Momordica charantia OX=3673 GN=LOC111015653 PE=3 SV=1)

HSP 1 Score: 193.0 bits (489), Expect = 1.4e-45
Identity = 109/168 (64.88%), Postives = 109/168 (64.88%), Query Frame = 0

Query: 58  RKKVHRYVIFMIDEKKKEVVVEKTGSPAESFDDFTASLPENDYRYAVFDFDFVTSENYQK 117
           RKKVHRYVIFMIDEKKKEVVVEKTGSPAESFDDFTASLPENDYRYAVFDFDFVTSENYQK
Sbjct: 16  RKKVHRYVIFMIDEKKKEVVVEKTGSPAESFDDFTASLPENDYRYAVFDFDFVTSENYQK 75

Query: 118 SKIFFISWFNLQLLVPLVLKEYCIYFLELSLLLVLQGIGNYLPIFDTLSSRGTHPVCSSI 177
           SKIFFISW                                                    
Sbjct: 76  SKIFFISW---------------------------------------------------- 124

Query: 178 PDFLHFLSPSVSRIRSKMLYATSKDRFRRELEGIHYEIQATDPTEDGY 226
                  SPSVSRIRSKMLYATSKDRFRRELEGIHYEIQATDPTEDGY
Sbjct: 136 -------SPSVSRIRSKMLYATSKDRFRRELEGIHYEIQATDPTEDGY 124

BLAST of Moc05g00930 vs. ExPASy TrEMBL
Match: A0A438HQT0 (Actin-depolymerizing factor 11 OS=Vitis vinifera OX=29760 GN=ADF11_1 PE=4 SV=1)

HSP 1 Score: 181.8 bits (460), Expect = 3.2e-42
Identity = 110/183 (60.11%), Postives = 124/183 (67.76%), Query Frame = 0

Query: 49  LGILEHIH------MRKKVHRYVIFMIDEKKKEVVVEKTGSPAESFDDFTASLPENDYRY 108
           +G+ +H         RKKVHRYVIF IDEKKKEVVVEKTG PAES+DDFTASLPEND RY
Sbjct: 55  MGVADHCKATYLELQRKKVHRYVIFKIDEKKKEVVVEKTGGPAESYDDFTASLPENDCRY 114

Query: 109 AVFDFDFVTSENYQKSKIFFISW---FNLQLLVPLVLKEYCIYFLELSLLLVLQGIGNYL 168
           A++DFDFVTSEN QKSKIFFI+W    N QL  P  L   C+  +  S   ++       
Sbjct: 115 AIYDFDFVTSENCQKSKIFFIAWQWSNNFQLFDP-SLSFPCLQEMTFSCSCLISA----Q 174

Query: 169 PIFDTLSSRGTHPVCSSIPDFLHFLSPSVSRIRSKMLYATSKDRFRRELEGIHYEIQATD 223
            I   L+  G             +LSPSVSRIR+KMLYATSKDRFRRELEGIHYEIQATD
Sbjct: 175 LIASVLAGGGV------------YLSPSVSRIRAKMLYATSKDRFRRELEGIHYEIQATD 220

BLAST of Moc05g00930 vs. ExPASy TrEMBL
Match: A0A438DD12 (Actin-depolymerizing factor 11 OS=Vitis vinifera OX=29760 GN=ADF11_0 PE=4 SV=1)

HSP 1 Score: 181.8 bits (460), Expect = 3.2e-42
Identity = 113/191 (59.16%), Postives = 126/191 (65.97%), Query Frame = 0

Query: 49  LGILEHIH------MRKKVHRYVIFMIDEKKKEVVVEKTGSPAESFDDFTASLPENDYRY 108
           +G+ +H         RKKVHRYVIF IDEKKKEVVVEKTG PAES+DDFTASLPEND RY
Sbjct: 81  MGVADHCKATYLELQRKKVHRYVIFKIDEKKKEVVVEKTGGPAESYDDFTASLPENDCRY 140

Query: 109 AVFDFDFVTSENYQKSKIFFISW---FNLQLLVPLV----LKEY---CIYFLELSLLL-V 168
           A++DFDFVTSEN QKSKIFFI+W    N QL  P +    L+E    C   +   L+  V
Sbjct: 141 AIYDFDFVTSENCQKSKIFFIAWQWSNNFQLFDPSLSFPCLQEMTFSCSCLISAQLIASV 200

Query: 169 LQGIGNYLPIFDTLSSRGTHPVCSSIPDFLHFLSPSVSRIRSKMLYATSKDRFRRELEGI 223
           L G+G Y                         LSPSVSRIR+KMLYATSKDRFRRELEGI
Sbjct: 201 LAGVGVY-------------------------LSPSVSRIRAKMLYATSKDRFRRELEGI 246

BLAST of Moc05g00930 vs. ExPASy TrEMBL
Match: W9SE02 (Actin-depolymerizing factor OS=Morus notabilis OX=981085 GN=L484_021423 PE=4 SV=1)

HSP 1 Score: 178.7 bits (452), Expect = 2.7e-41
Identity = 105/167 (62.87%), Postives = 118/167 (70.66%), Query Frame = 0

Query: 58  RKKVHRYVIFMIDEKKKEVVVEKTGSPAESFDDFTASLPENDYRYAVFDFDFVTSENYQK 117
           RKKV RYVIF IDEKKKEV+VEKTG PAESFDDF ASLPEND RYAV+DFDFVTSEN QK
Sbjct: 16  RKKVFRYVIFKIDEKKKEVMVEKTGGPAESFDDFAASLPENDCRYAVYDFDFVTSENCQK 75

Query: 118 SKIFFISWFNLQLLVPLVLKEYCIYFLELSLLLVLQGIGNYLPI--FDTLSSRGTHPVCS 177
           SKIFFI+     +  P  L    +YF+ L         G+ LP+  F  L       +  
Sbjct: 76  SKIFFIACKGKDVENPSELSSASLYFMPLD--------GSPLPVYFFPPL-------IFL 135

Query: 178 SIPDFLHFLSPSVSRIRSKMLYATSKDRFRRELEGIHYEIQATDPTE 223
           + P  L   SPSVSRIR+KMLYATSKD+FRREL+GIHYEIQATDPTE
Sbjct: 136 TFPGVLLLRSPSVSRIRAKMLYATSKDKFRRELDGIHYEIQATDPTE 167

BLAST of Moc05g00930 vs. TAIR 10
Match: AT2G31200.1 (actin depolymerizing factor 6 )

HSP 1 Score: 145.6 bits (366), Expect = 4.8e-35
Identity = 83/165 (50.30%), Postives = 94/165 (56.97%), Query Frame = 0

Query: 58  RKKVHRYVIFMIDEKKKEVVVEKTGSPAESFDDFTASLPENDYRYAVFDFDFVTSENYQK 117
           RKK HRYV+F IDE KKEVVVEKTG+P ES+DDF ASLP+ND RYAV+DFDFVTSEN QK
Sbjct: 30  RKKTHRYVVFKIDESKKEVVVEKTGNPTESYDDFLASLPDNDCRYAVYDFDFVTSENCQK 89

Query: 118 SKIFFISWFNLQLLVPLVLKEYCIYFLELSLLLVLQGIGNYLPIFDTLSSRGTHPVCSSI 177
           SKIFF +W                                                    
Sbjct: 90  SKIFFFAW---------------------------------------------------- 135

Query: 178 PDFLHFLSPSVSRIRSKMLYATSKDRFRRELEGIHYEIQATDPTE 223
                  SPS S IR+K+LY+TSKD+  REL+GIHYEIQATDPTE
Sbjct: 150 -------SPSTSGIRAKVLYSTSKDQLSRELQGIHYEIQATDPTE 135

BLAST of Moc05g00930 vs. TAIR 10
Match: AT4G00680.1 (actin depolymerizing factor 8 )

HSP 1 Score: 122.9 bits (307), Expect = 3.4e-28
Identity = 66/164 (40.24%), Postives = 91/164 (55.49%), Query Frame = 0

Query: 59  KKVHRYVIFMIDEKKKEVVVEKTGSPAESFDDFTASLPENDYRYAVFDFDFVTSENYQKS 118
           K+ +R+++F IDEK ++V +EK G+P E++DDFT+S+P+++ RYAV+DFDF T +N QKS
Sbjct: 24  KRTYRFIVFKIDEKAQQVQIEKLGNPEETYDDFTSSIPDDECRYAVYDFDFTTEDNCQKS 83

Query: 119 KIFFISWFNLQLLVPLVLKEYCIYFLELSLLLVLQGIGNYLPIFDTLSSRGTHPVCSSIP 178
           KIFFI+W                                                     
Sbjct: 84  KIFFIAW----------------------------------------------------- 128

Query: 179 DFLHFLSPSVSRIRSKMLYATSKDRFRRELEGIHYEIQATDPTE 223
                 SP  SR+RSKMLYA+SKDRF+RE+EGI  E+QATDP+E
Sbjct: 144 ------SPDTSRVRSKMLYASSKDRFKREMEGIQVELQATDPSE 128

BLAST of Moc05g00930 vs. TAIR 10
Match: AT4G34970.1 (actin depolymerizing factor 9 )

HSP 1 Score: 122.9 bits (307), Expect = 3.4e-28
Identity = 69/167 (41.32%), Postives = 88/167 (52.69%), Query Frame = 0

Query: 59  KKVHRYVIFMIDEKKKEVVVEKTGSPAESFDDFTASLPENDYRYAVFDFDFVTSENYQKS 118
           KKVHRYV++ ++EK ++V V+K G+  ES+DD  ASLPE+D RYAVFDFD+VT +N + S
Sbjct: 26  KKVHRYVVYKLEEKSRKVTVDKVGAAGESYDDLAASLPEDDCRYAVFDFDYVTVDNCRMS 85

Query: 119 KIFFISWFNLQLLVPLVLKEYCIYFLELSLLLVLQGIGNYLPIFDTLSSRGTHPVCSSIP 178
           KIFFI+W                                                     
Sbjct: 86  KIFFITW----------------------------------------------------- 133

Query: 179 DFLHFLSPSVSRIRSKMLYATSKDRFRRELEGIHYEIQATDPTEDGY 226
                 SP  SRIR KM+YATSK   RR L+G+HYE+QATDPTE G+
Sbjct: 146 ------SPEASRIREKMMYATSKSGLRRVLDGVHYELQATDPTEMGF 133

BLAST of Moc05g00930 vs. TAIR 10
Match: AT2G16700.1 (actin depolymerizing factor 5 )

HSP 1 Score: 121.7 bits (304), Expect = 7.5e-28
Identity = 71/167 (42.51%), Postives = 87/167 (52.10%), Query Frame = 0

Query: 59  KKVHRYVIFMIDEKKKEVVVEKTGSPAESFDDFTASLPENDYRYAVFDFDFVTSENYQKS 118
           KKVHRY++F I+EK ++V V+K G   ES+ D   SLP +D RYAVFDFDFVT +N +KS
Sbjct: 28  KKVHRYIVFKIEEKSRKVTVDKVGGAGESYHDLEDSLPVDDCRYAVFDFDFVTVDNCRKS 87

Query: 119 KIFFISWFNLQLLVPLVLKEYCIYFLELSLLLVLQGIGNYLPIFDTLSSRGTHPVCSSIP 178
           KIFFI+W                                                     
Sbjct: 88  KIFFIAW----------------------------------------------------- 135

Query: 179 DFLHFLSPSVSRIRSKMLYATSKDRFRRELEGIHYEIQATDPTEDGY 226
                 SP  S+IR+K+LYATSKD  RR LEGIHYE+QATDPTE G+
Sbjct: 148 ------SPEASKIRAKILYATSKDGLRRVLEGIHYELQATDPTEMGF 135

BLAST of Moc05g00930 vs. TAIR 10
Match: AT2G16700.2 (actin depolymerizing factor 5 )

HSP 1 Score: 121.7 bits (304), Expect = 7.5e-28
Identity = 71/167 (42.51%), Postives = 87/167 (52.10%), Query Frame = 0

Query: 59  KKVHRYVIFMIDEKKKEVVVEKTGSPAESFDDFTASLPENDYRYAVFDFDFVTSENYQKS 118
           KKVHRY++F I+EK ++V V+K G   ES+ D   SLP +D RYAVFDFDFVT +N +KS
Sbjct: 17  KKVHRYIVFKIEEKSRKVTVDKVGGAGESYHDLEDSLPVDDCRYAVFDFDFVTVDNCRKS 76

Query: 119 KIFFISWFNLQLLVPLVLKEYCIYFLELSLLLVLQGIGNYLPIFDTLSSRGTHPVCSSIP 178
           KIFFI+W                                                     
Sbjct: 77  KIFFIAW----------------------------------------------------- 124

Query: 179 DFLHFLSPSVSRIRSKMLYATSKDRFRRELEGIHYEIQATDPTEDGY 226
                 SP  S+IR+K+LYATSKD  RR LEGIHYE+QATDPTE G+
Sbjct: 137 ------SPEASKIRAKILYATSKDGLRRVLEGIHYELQATDPTEMGF 124

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022146448.12.2e-4561.20actin-depolymerizing factor-like isoform X2 [Momordica charantia][more]
XP_022146440.12.8e-4564.88actin-depolymerizing factor-like isoform X1 [Momordica charantia][more]
RVW86811.16.6e-4260.11Actin-depolymerizing factor 11 [Vitis vinifera][more]
RVW33367.16.6e-4259.16Actin-depolymerizing factor 11 [Vitis vinifera][more]
EXC01786.15.5e-4162.87Actin-depolymerizing factor [Morus notabilis][more]
Match NameE-valueIdentityDescription
Q8SAG34.6e-3855.15Actin-depolymerizing factor OS=Vitis vinifera OX=29760 PE=2 SV=1[more]
Q2QLT81.4e-3451.52Actin-depolymerizing factor 11 OS=Oryza sativa subsp. japonica OX=39947 GN=ADF11... [more]
Q9ZSK26.8e-3450.30Actin-depolymerizing factor 6 OS=Arabidopsis thaliana OX=3702 GN=ADF6 PE=1 SV=1[more]
Q9AY764.9e-3248.78Actin-depolymerizing factor 2 OS=Oryza sativa subsp. japonica OX=39947 GN=ADF2 P... [more]
Q337A54.6e-3044.24Actin-depolymerizing factor 10 OS=Oryza sativa subsp. japonica OX=39947 GN=ADF10... [more]
Match NameE-valueIdentityDescription
A0A6J1CYL71.1e-4561.20actin-depolymerizing factor-like isoform X2 OS=Momordica charantia OX=3673 GN=LO... [more]
A0A6J1CX921.4e-4564.88actin-depolymerizing factor-like isoform X1 OS=Momordica charantia OX=3673 GN=LO... [more]
A0A438HQT03.2e-4260.11Actin-depolymerizing factor 11 OS=Vitis vinifera OX=29760 GN=ADF11_1 PE=4 SV=1[more]
A0A438DD123.2e-4259.16Actin-depolymerizing factor 11 OS=Vitis vinifera OX=29760 GN=ADF11_0 PE=4 SV=1[more]
W9SE022.7e-4162.87Actin-depolymerizing factor OS=Morus notabilis OX=981085 GN=L484_021423 PE=4 SV=... [more]
Match NameE-valueIdentityDescription
AT2G31200.14.8e-3550.30actin depolymerizing factor 6 [more]
AT4G00680.13.4e-2840.24actin depolymerizing factor 8 [more]
AT4G34970.13.4e-2841.32actin depolymerizing factor 9 [more]
AT2G16700.17.5e-2842.51actin depolymerizing factor 5 [more]
AT2G16700.27.5e-2842.51actin depolymerizing factor 5 [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (OHB3-1) v2
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002108Actin-depolymerising factor homology domainSMARTSM00102adf_2coord: 46..224
e-value: 8.8E-16
score: 68.4
IPR002108Actin-depolymerising factor homology domainPFAMPF00241Cofilin_ADFcoord: 54..125
e-value: 7.7E-17
score: 61.2
coord: 182..222
e-value: 3.3E-8
score: 33.3
IPR002108Actin-depolymerising factor homology domainPROSITEPS51263ADF_Hcoord: 40..225
score: 16.866302
IPR029006ADF-H/Gelsolin-like domain superfamilyGENE3D3.40.20.10Severincoord: 49..224
e-value: 2.8E-39
score: 136.3
NoneNo IPR availablePANTHERPTHR11913:SF83ACTIN-DEPOLYMERIZING FACTOR-LIKEcoord: 57..126
coord: 174..222
NoneNo IPR availableSUPERFAMILY55753Actin depolymerizing proteinscoord: 182..222
NoneNo IPR availableSUPERFAMILY55753Actin depolymerizing proteinscoord: 58..125
IPR017904ADF/CofilinPANTHERPTHR11913COFILIN-RELATEDcoord: 57..126
coord: 174..222
IPR017904ADF/CofilinCDDcd11286ADF_cofilin_likecoord: 53..222
e-value: 3.33059E-35
score: 119.969

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Moc05g00930.1Moc05g00930.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0030042 actin filament depolymerization
cellular_component GO:0015629 actin cytoskeleton
molecular_function GO:0003779 actin binding