Moc05g00660 (gene) Bitter gourd (OHB3-1) v2

Overview


Name	Moc05g00660
Type	gene
Organism	Momordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
Description	enoyl-CoA delta isomerase 2, peroxisomal-like
Location	chr5: 498409 .. 501492 (+)
RNA-Seq Expression	Moc05g00660
Synteny	Moc05g00660

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGTGCACTCTTGAGAAGCGTGGCGATGTCTTCATCCTCACTCTCACCGGCGACAACGAGCACTGGATCGGCCCACCCTTCGTTTCCTCCCTCCTCTCTCTTCTTTCCCAAGTCAAGTCCCAAGCCACGCGCGGCTCGGTTCTCCTCACCACTTCCCATGGCCGCTTCTTCTCCAACGGCTTCGACCTTCCTTGGGCCCAAGCCGCCGGCTCCAAATCTGCCGCCACTGACCGCCTAGCCCACATGGTCCACATCTTCAAGCCAGTTGTTGCCGCCCTCCTCTCCCTCCCCATGCCCACCATCGCCGCTGTCTCCGGCCACGCCGCCGCCGCCGGCTTCCTTCTCGCGCTCGCCCACGATTTCGTCCTCATGAGGCGCGATAAAGGCGTGCTCTACATGAGTGAGGTTTGATTAATTACCACTTTTTTTAGATATTATATATAATTAAATTTATGTTCGAATTTTTGTATTGTAATTTTCCTCATATATAATATTAAGTTATCATAATCCATCCCCTTGGACTAAGTTCTTATTGATAATCTAATCATGCTTGAGACTTAACTTCTTTTTTTTTAACTGATTAATCAGTGTTTCACCTTAGTCCAATTTTAGAAATTTCAATTTAATTACTCTAGTTTTGTTTTTTTAAGTACAAAACGAATCTAAGGAACGATGTCTAGTTTTATATGTTTTAAATTTTAAACTGACGATGCATAAGTTCAAATGGCTATGACACTTCTATCCCACCCTAATTGGGTTTTAAAAAAAAGTTAACCATAAGTATTAAATTGGAACTATTGGTTAAAGATTAATAAACCACTATTCAACTTAAAAAAGCTTAAAAAGCTTAAGCTGGATCAGGTAAATTTATCCTATGATTAGAGAGGAAACAATATTGATTCAAATACAAGACCTCCTCCTTTTTGTTAAGGATAAATAGACCCTATGTTAGAGTAGAATATTCTGTATTATATTGTATATGTAATTATTTAGTTTCCTTGTTTATAGGCTCCAATGATTAGCTCAAATGATTTTGGTGAGTCTAGTCTTATATATACAGCAGGGAGGTTAATGAGAAGGGAGGAGATTTTGGTGAGTCTAGTCTTATATATACAACAGAGAGGTTAATGAGAAGGGAGGAGATTTTGATTTTGATGGGTTAGATAAATTTAATTTATATATATTTTGATGGGTTGTATTTTGTCTACCCTACTCTTAATAATATTTAATGCACATTCTAATTTACTCTCAAGCTTAATTAATATATATACATATATAAATTTTTCTCACCCCTATTTATGGGTGCTTTTAGGTTAGCTAAATTTAATTTTTATATATAGATTCTCACCGATATTTATGGACATTTAACATTTCCATTTATTGATAGGTTGATTTGGGTTTAACCATGCCCGACTACTTTGCGGCATTGATGAGGTCGAAAATCGGGTCAGCTTCAGTCCGGCGAGATGTGTTGCTGACGGGGATGAAGGTGAACGGCGAATCGGCGGTGAAGTTGGGAATCGCCGATGCAGCCCATGACGGCGAGGAGGGGTTGATGGAGGCTGCGGTGGCGATGGGACAAAAATTGGCGAAGAGAAAGTGGGAGGGGGGAGCTTACGCACAGATTAGGAAGAGTCTGTATCCTGAAATGACTCCGTTACTGGATGGATTAAATGGAAAGATGTAAGCAGTAAGCCAAATGAACGACATGTCGTCTTGGTGGATTTGTTTGTTGGAGTGTTTTGGTTGATACGGTGTTGTAAAACTCAATAATTCTGGACTCTGTTTTCATCGATTAGCTGGAGGAATTTTGTTTTTCTCTGTTCTTTTGCTCCCTGTGATAATGCCAAAACCAAAAGCAAAGTGTGTTTTAAATAGCATGTTATTTGTTGAGTTTCTTCCATCTTGTTCTAAGCTTTGGACGGATTAGGAGCCATGGAATGTAGTAGTTTATTAGAAAGGTTATGACTTTATTGCTACATTTTCTATTATGATACAATCTGTATCAGCCAATTATTCATATATATATCATATATGTACCTTTCGTGAGCAAGAGGATATTTGATAGAGATATAGTGATAGTAGAATTGTCCACGCTCTTGTACGTCGAGCGTATATATATTTTTTATATAATATATAGCGGTGGGGGTTTGAACTCATGATCCGTTGGTCAGAGATAATGTCTCAACCACTTGAACTATGTTCGATTTAACTTAAAGGTAGATACTTAGAGTGGGCTAAAAAGCCACAAATTTATTTGGAGTATAGGCATTAATTATAATTGCCTAACATTTTTAAAAGAAGAAAAAAAAAAGCTTCCGTCAGTTATAGCTAGCTTGATAATTGATTGGTTAGAGAGTATCGACCAATATTCCCGAAACCGTGCAGCGTTGAGAAGCGCGGCGAGCTTTTCATTCTAACTCTGACCGGCAACGACGAGCACCGGCTGAGCCCACCCTTCATCGAATCCATCCTGGCCGCTCTTTTCCAAGTCAAGGCCCAAGCCTCCCTCGGTTCAGTTCTCGTCACGACTGCCCACGGCAAATTCTTCTCCAACGGCCTGGATCTCCCTTGGGCCCTCGTTGCGGCCTCCTTGCCTGTGGCCCAGGCCCGCCTCGGCCACATGCTTCACATTTTCAAGCGCCTCGTCACGGAACTCTTTTCCTTTCACATGCCCACCATAGCCGCTCTGACGGGGCATGCTGTCGCCGCCGGCTTCATGCTGGCGCTGGCCCACGACTATGTGATGATGAGGAGCGACCGAGGCGTGGTGTACATGAGCGAGCTAGATCTAGGTGCGAGTCAGCCCGAGTTCTTCATGGTGCTGGTTAGGTCTAAGATTGGGCCGAGTTGGCCCTGCGTGACGTGCTGAGGGGCCTGAAAGTGAAGGGCGAATGTGAAGAATGAAGATGGGGATCGTTGAGTCGGCCCACGACGGCGAGGACGGCGCGTGAAGGCTGCAGTGCGGCTTGGGGAGCAGCTCGCCTGCAGAAACTGAAAAGGTACGGAGATCAGGAAAAGTTTCCTGAGATCTCTAGATTGCTTGGATTGCCGTCCAATGCCATAGCAATTTCCAAGATCTGA

mRNA sequence

ATGTGCACTCTTGAGAAGCGTGGCGATGTCTTCATCCTCACTCTCACCGGCGACAACGAGCACTGGATCGGCCCACCCTTCGTTTCCTCCCTCCTCTCTCTTCTTTCCCAAGTCAAGTCCCAAGCCACGCGCGGCTCGGTTCTCCTCACCACTTCCCATGGCCGCTTCTTCTCCAACGGCTTCGACCTTCCTTGGGCCCAAGCCGCCGGCTCCAAATCTGCCGCCACTGACCGCCTAGCCCACATGGTCCACATCTTCAAGCCAGTTGTTGCCGCCCTCCTCTCCCTCCCCATGCCCACCATCGCCGCTGTCTCCGGCCACGCCGCCGCCGCCGGCTTCCTTCTCGCGCTCGCCCACGATTTCGTCCTCATGAGGCGCGATAAAGGCGTGCTCTACATGAGTGAGGTTGATTTGGGTTTAACCATGCCCGACTACTTTGCGGCATTGATGAGGTCGAAAATCGGGTCAGCTTCAGTCCGGCGAGATGTGTTGCTGACGGGGATGAAGGTGAACGGCGAATCGGCGGTGAAGTTGGGAATCGCCGATGCAGCCCATGACGGCGAGGAGGGGTTGATGGAGGCTGCGGTGGCGATGGGACAAAAATTGGCGAAGAGAAAGTGGGAGGGGGGAGCTTACGCACAGATTAGGAAGAGTCTGTATCCTGAAATGACTCCGTTACTGGATGGATTAAATGGAAAGATAGAGTATCGACCAATATTCCCGAAACCGTGCAGCGTTGAGAAGCGCGGCGAGCTTTTCATTCTAACTCTGACCGGCAACGACGAGCACCGGCTGAGCCCACCCTTCATCGAATCCATCCTGGCCGCTCTTTTCCAAGTCAAGGCCCAAGCCTCCCTCGGTTCAGTTCTCGTCACGACTGCCCACGGCAAATTCTTCTCCAACGGCCTGGATCTCCCTTGGGCCCTCGTTGCGGCCTCCTTGCCTGTGGCCCAGGCCCGCCTCGGCCACATGCTTCACATTTTCAAGCGCCTCGTCACGGAACTCTTTTCCTTTCACATGCCCACCATAGCCGCTCTGACGGGGCATGCTGTCGCCGCCGGCTTCATGCTGGCGCTGGCCCACGACTATGTGATGATGAGGAGCGACCGAGGCGTGGTGTACATGAGCGAGCTAGATCTAGGTGCGAGTCAGCCCGAGTTCTTCATGGTGCTGGTTAGATGGGGATCGTTGAGTCGGCCCACGACGGCGAGGACGGCGCGTGAAGGCTGCAGTGCGGCTTGGGGAGCAGCTCGCCTGCAGAAACTGAAAAGGTACGGAGATCAGGAAAAGTTTCCTGAGATCTCTAGATTGCTTGGATTGCCGTCCAATGCCATAGCAATTTCCAAGATCTGA

Coding sequence (CDS)

ATGTGCACTCTTGAGAAGCGTGGCGATGTCTTCATCCTCACTCTCACCGGCGACAACGAGCACTGGATCGGCCCACCCTTCGTTTCCTCCCTCCTCTCTCTTCTTTCCCAAGTCAAGTCCCAAGCCACGCGCGGCTCGGTTCTCCTCACCACTTCCCATGGCCGCTTCTTCTCCAACGGCTTCGACCTTCCTTGGGCCCAAGCCGCCGGCTCCAAATCTGCCGCCACTGACCGCCTAGCCCACATGGTCCACATCTTCAAGCCAGTTGTTGCCGCCCTCCTCTCCCTCCCCATGCCCACCATCGCCGCTGTCTCCGGCCACGCCGCCGCCGCCGGCTTCCTTCTCGCGCTCGCCCACGATTTCGTCCTCATGAGGCGCGATAAAGGCGTGCTCTACATGAGTGAGGTTGATTTGGGTTTAACCATGCCCGACTACTTTGCGGCATTGATGAGGTCGAAAATCGGGTCAGCTTCAGTCCGGCGAGATGTGTTGCTGACGGGGATGAAGGTGAACGGCGAATCGGCGGTGAAGTTGGGAATCGCCGATGCAGCCCATGACGGCGAGGAGGGGTTGATGGAGGCTGCGGTGGCGATGGGACAAAAATTGGCGAAGAGAAAGTGGGAGGGGGGAGCTTACGCACAGATTAGGAAGAGTCTGTATCCTGAAATGACTCCGTTACTGGATGGATTAAATGGAAAGATAGAGTATCGACCAATATTCCCGAAACCGTGCAGCGTTGAGAAGCGCGGCGAGCTTTTCATTCTAACTCTGACCGGCAACGACGAGCACCGGCTGAGCCCACCCTTCATCGAATCCATCCTGGCCGCTCTTTTCCAAGTCAAGGCCCAAGCCTCCCTCGGTTCAGTTCTCGTCACGACTGCCCACGGCAAATTCTTCTCCAACGGCCTGGATCTCCCTTGGGCCCTCGTTGCGGCCTCCTTGCCTGTGGCCCAGGCCCGCCTCGGCCACATGCTTCACATTTTCAAGCGCCTCGTCACGGAACTCTTTTCCTTTCACATGCCCACCATAGCCGCTCTGACGGGGCATGCTGTCGCCGCCGGCTTCATGCTGGCGCTGGCCCACGACTATGTGATGATGAGGAGCGACCGAGGCGTGGTGTACATGAGCGAGCTAGATCTAGGTGCGAGTCAGCCCGAGTTCTTCATGGTGCTGGTTAGATGGGGATCGTTGAGTCGGCCCACGACGGCGAGGACGGCGCGTGAAGGCTGCAGTGCGGCTTGGGGAGCAGCTCGCCTGCAGAAACTGAAAAGGTACGGAGATCAGGAAAAGTTTCCTGAGATCTCTAGATTGCTTGGATTGCCGTCCAATGCCATAGCAATTTCCAAGATCTGA

Protein sequence

MCTLEKRGDVFILTLTGDNEHWIGPPFVSSLLSLLSQVKSQATRGSVLLTTSHGRFFSNGFDLPWAQAAGSKSAATDRLAHMVHIFKPVVAALLSLPMPTIAAVSGHAAAAGFLLALAHDFVLMRRDKGVLYMSEVDLGLTMPDYFAALMRSKIGSASVRRDVLLTGMKVNGESAVKLGIADAAHDGEEGLMEAAVAMGQKLAKRKWEGGAYAQIRKSLYPEMTPLLDGLNGKIEYRPIFPKPCSVEKRGELFILTLTGNDEHRLSPPFIESILAALFQVKAQASLGSVLVTTAHGKFFSNGLDLPWALVAASLPVAQARLGHMLHIFKRLVTELFSFHMPTIAALTGHAVAAGFMLALAHDYVMMRSDRGVVYMSELDLGASQPEFFMVLVRWGSLSRPTTARTAREGCSAAWGAARLQKLKRYGDQEKFPEISRLLGLPSNAIAISKI

Homology

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BLAST of Moc05g00660 vs. NCBI nr
Match: KAG7018780.1 (Enoyl-CoA delta isomerase 3, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 534.3 bits (1375), Expect = 1.0e-147
Identity = 297/472 (62.92%), Postives = 340/472 (72.03%), Query Frame = 0


Query: 1   MCTLEKRGDVFILTLTGDNEHWIGPPFVSSLLSLLSQVKSQATRGSVLLTTSHGRFFSNG 60
           MCTLEKRG VFILTLTGDNEHWIGP F+SS LSLL+QVKSQATRGSVLLTTSHGRFFSNG
Sbjct: 1   MCTLEKRGGVFILTLTGDNEHWIGPTFISSFLSLLAQVKSQATRGSVLLTTSHGRFFSNG 60

Query: 61  FDLPWAQAAGSKSAATDRLAHMVHIFKPVVAALLSLPMPTIAAVSGHAAAAGFLLALAHD 120
           FDLPWA  A SKSAATDR+ H+VHIFK V AALLSLPMPTIAAVSGHA+AAGF+LAL HD
Sbjct: 61  FDLPWALTAASKSAATDRILHLVHIFKQVAAALLSLPMPTIAAVSGHASAAGFILALYHD 120

Query: 121 FVLMRRDKGVLYMSEVDLGLTMPDYFAALMRSKIGSASVRRDVLLTGMKVNGESAVKLGI 180
           ++LMRRDKG LYMSE+D+GL  PDY AALMRSKI SASVRRDVLL GMK+NGE+A KLGI
Sbjct: 121 YLLMRRDKGFLYMSEIDMGLPFPDYIAALMRSKISSASVRRDVLLAGMKINGETATKLGI 180

Query: 181 ADAAHDGEEGLMEAAVAMGQKLAKRKWEGGAYAQIRKSLYPEMTPLLDGLNGKIEYRPIF 240
           ADAA++GEE LM+AAVAM + LAKRKW+G                               
Sbjct: 181 ADAAYNGEEMLMDAAVAMAETLAKRKWDG------------------------------- 240

Query: 241 PKPCSVEKRGELFILTLTGNDEHRLSPPFIESILAALFQVKAQASLGSVLVTTAHGKFFS 300
            + C+VEKRG LFILTLTG+DEHRLSP +I SIL AL +VKA+AS GSVL+TT+HGKFFS
Sbjct: 241 -EMCTVEKRGNLFILTLTGDDEHRLSPSYIASILKALSKVKAEASPGSVLITTSHGKFFS 300

Query: 301 NGLDLPWALVAASLPVAQARLGHMLHIFKRLVTELFSFHMPTIAALTGHAVAAGFMLALA 360
           NG DL WA  A S+  A  RL HM+ IFK +V EL SF MPTIAA+ GHA AAGF+LAL+
Sbjct: 301 NGFDLSWAQAAGSVSAAAERLHHMVQIFKPVVAELLSFPMPTIAAIAGHAAAAGFLLALS 360

Query: 361 HDYVMMRSDRGVVYMSELDLGASQPEFFMVLV--RWGSLS------------------RP 420
           HDY++MRSDRGV+YMSE+DLG + P++F  L   + GS S                  R 
Sbjct: 361 HDYLIMRSDRGVLYMSEVDLGLTLPDYFSALFKSKIGSSSVRRDVLLTGMKVKGEPAVRM 420

Query: 421 TTARTAREGCSAAWGAA-RL--QKLKRYGDQEKF--------PEISRLLGLP 442
               +A EG      AA RL  Q  +R  + E +        PEISRLLGLP
Sbjct: 421 GIVESAHEGADGVMKAAVRLGEQLAERNWNGEPYAEIRKSLHPEISRLLGLP 440

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BLAST of Moc05g00660 vs. NCBI nr
Match: XP_022143632.1 (enoyl-CoA delta isomerase 2, peroxisomal-like [Momordica charantia])

HSP 1 Score: 456.8 bits (1174), Expect = 2.1e-124
Identity = 233/234 (99.57%), Postives = 234/234 (100.00%), Query Frame = 0


Query: 1   MCTLEKRGDVFILTLTGDNEHWIGPPFVSSLLSLLSQVKSQATRGSVLLTTSHGRFFSNG 60
           MCTLEKRGDVFILTLTGDNEHWIGPPFVSSLLSLLSQVKSQATRGSVLLTTSHGRFFSNG
Sbjct: 25  MCTLEKRGDVFILTLTGDNEHWIGPPFVSSLLSLLSQVKSQATRGSVLLTTSHGRFFSNG 84

Query: 61  FDLPWAQAAGSKSAATDRLAHMVHIFKPVVAALLSLPMPTIAAVSGHAAAAGFLLALAHD 120
           FDLPWAQAAGSKSAATDRLAHMVHIFKPVVAALLSLPMPTIAAVSGHAAAAGFLLALAHD
Sbjct: 85  FDLPWAQAAGSKSAATDRLAHMVHIFKPVVAALLSLPMPTIAAVSGHAAAAGFLLALAHD 144

Query: 121 FVLMRRDKGVLYMSEVDLGLTMPDYFAALMRSKIGSASVRRDVLLTGMKVNGESAVKLGI 180
           FVLMRRDKGVLYMSEVDLGLTMPDYFAALMRSKIGSASVRRDVLLTGMKVNGESAVKLGI
Sbjct: 145 FVLMRRDKGVLYMSEVDLGLTMPDYFAALMRSKIGSASVRRDVLLTGMKVNGESAVKLGI 204

Query: 181 ADAAHDGEEGLMEAAVAMGQKLAKRKWEGGAYAQIRKSLYPEMTPLLDGLNGKI 235
           ADAAHDGEEGLMEAAVAMGQKLAKRKWEGGAYAQIRKSLYPEMTPLLDGLNGK+
Sbjct: 205 ADAAHDGEEGLMEAAVAMGQKLAKRKWEGGAYAQIRKSLYPEMTPLLDGLNGKM 258

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BLAST of Moc05g00660 vs. NCBI nr
Match: KAF9677702.1 (hypothetical protein SADUNF_Sadunf08G0135000 [Salix dunnii])

HSP 1 Score: 449.1 bits (1154), Expect = 4.4e-122
Identity = 233/405 (57.53%), Postives = 292/405 (72.10%), Query Frame = 0


Query: 1   MCTLEK-RGDVFILTLTGDNEHWIGPPFVSSLLSLLSQVKSQATRGSVLLTTSHGRFFSN 60
           MCTLEK   ++F+LTLTGD+EH + P  + S+LS L   K +AT GSVL+TTS G+FFSN
Sbjct: 1   MCTLEKPYNNIFVLTLTGDDEHRLNPTLIGSILSALRDAKEKATSGSVLITTSRGKFFSN 60

Query: 61  GFDLPWAQAAGSKSAATDRLAHMVHIFKPVVAALLSLPMPTIAAVSGHAAAAGFLLALAH 120
           GFDL WA+AAGSK  A +RL  MV + KPVVA L+SLPMPT+AA++GHAAA G +LAL+H
Sbjct: 61  GFDLAWARAAGSKPKAIERLHQMVELLKPVVAELISLPMPTVAAINGHAAAGGLILALSH 120

Query: 121 DFVLMRRDKGVLYMSEVDLGLTMPDYFAALMRSKIGSASVRRDVLLTGMKVNGESAVKLG 180
           D+V M+RD+GVLYMSEVD+GLT+PDYF AL+R+K+GS S RRDVLL G KV G+ AV +G
Sbjct: 121 DYVYMKRDRGVLYMSEVDIGLTIPDYFNALIRAKVGSPSARRDVLLRGAKVRGDEAVSMG 180

Query: 181 IADAAHDGEEGLMEAAVAMGQKLAKRKWEGGAYAQIRKSLYPEMTPLLDGLNGKIEYRPI 240
           I + AHD EE L EA +  G++LA RKW+G  Y +IRK LYPE+   L GL G       
Sbjct: 181 IVEGAHDNEERLREATMNHGKQLASRKWDGVVYKEIRKRLYPELCVAL-GLGG------- 240

Query: 241 FPKPCSVEKRGELFILTLTGNDEHRLSPPFIESILAALFQVKAQASLGSVLVTTAHGKFF 300
                  E    +F+LTLTG+DEHRL+P  I SIL+AL   K QA+ GSVL+TT+ GKFF
Sbjct: 241 ------AETDNNIFVLTLTGDDEHRLNPTLIGSILSALRDAKEQATSGSVLITTSRGKFF 300

Query: 301 SNGLDLPWALVAASLPVAQARLGHMLHIFKRLVTELFSFHMPTIAALTGHAVAAGFMLAL 360
           SNGLDL WA  A S P A  RL HM  +FK +V EL S  MPTIAA+ GHA A G +LAL
Sbjct: 301 SNGLDLAWAQAAGSKPKAIERLYHMAELFKPVVAELISLPMPTIAAINGHAAAGGLILAL 360

Query: 361 AHDYVMMRSDRGVVYMSELDLGASQPEFFMVLVRWGSLSRPTTAR 405
           +HDYV M+ DRGV+YMSE+DLG + P++ + L+R   +  P+  R
Sbjct: 361 SHDYVYMKRDRGVLYMSEVDLGLTIPDYVIALLR-AKIGSPSARR 390

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BLAST of Moc05g00660 vs. NCBI nr
Match: XP_022975055.1 (enoyl-CoA delta isomerase 2, peroxisomal-like [Cucurbita maxima] >XP_022975341.1 enoyl-CoA delta isomerase 2, peroxisomal-like [Cucurbita maxima])

HSP 1 Score: 401.0 bits (1029), Expect = 1.4e-107
Identity = 197/234 (84.19%), Postives = 220/234 (94.02%), Query Frame = 0


Query: 1   MCTLEKRGDVFILTLTGDNEHWIGPPFVSSLLSLLSQVKSQATRGSVLLTTSHGRFFSNG 60
           MCTLE+RG++FI+TLTGD+EHWIGPPF+SS LSLL+Q+ SQAT GSVL+TTSHGRFFSNG
Sbjct: 1   MCTLERRGNLFIITLTGDDEHWIGPPFISSFLSLLTQINSQATPGSVLITTSHGRFFSNG 60

Query: 61  FDLPWAQAAGSKSAATDRLAHMVHIFKPVVAALLSLPMPTIAAVSGHAAAAGFLLALAHD 120
           F LPWAQ+A SKSAA DR+ HMVHIFKPV+AALLSLPMPTIAAVSGHAAAAGF LAL+HD
Sbjct: 61  FHLPWAQSAASKSAARDRILHMVHIFKPVLAALLSLPMPTIAAVSGHAAAAGFALALSHD 120

Query: 121 FVLMRRDKGVLYMSEVDLGLTMPDYFAALMRSKIGSASVRRDVLLTGMKVNGESAVKLGI 180
           +VLMRRDKGVLYMSE+DLG T+PDYFAALMRSKIGSASVRRDVLL GMKVNGE+AVKLGI
Sbjct: 121 YVLMRRDKGVLYMSEIDLGFTLPDYFAALMRSKIGSASVRRDVLLAGMKVNGETAVKLGI 180

Query: 181 ADAAHDGEEGLMEAAVAMGQKLAKRKWEGGAYAQIRKSLYPEMTPLLDGLNGKI 235
            DAAHDGEE LMEAAVAMG+KLAKRKWEG AYA+IRKSLYPEM+PLL+ L+GK+
Sbjct: 181 TDAAHDGEEALMEAAVAMGEKLAKRKWEGEAYAEIRKSLYPEMSPLLNELSGKL 234

[Close]

BLAST of Moc05g00660 vs. NCBI nr
Match: XP_022942426.1 (enoyl-CoA delta isomerase 2, peroxisomal-like [Cucurbita moschata])

HSP 1 Score: 398.7 bits (1023), Expect = 6.8e-107
Identity = 196/234 (83.76%), Postives = 219/234 (93.59%), Query Frame = 0


Query: 1   MCTLEKRGDVFILTLTGDNEHWIGPPFVSSLLSLLSQVKSQATRGSVLLTTSHGRFFSNG 60
           MCTLE+RGD+FI+TLTGD+EHWIGPPF+SS LSLL+Q+ SQAT GSVL+TTSHGRFFSNG
Sbjct: 1   MCTLERRGDLFIITLTGDDEHWIGPPFISSFLSLLTQINSQATPGSVLITTSHGRFFSNG 60

Query: 61  FDLPWAQAAGSKSAATDRLAHMVHIFKPVVAALLSLPMPTIAAVSGHAAAAGFLLALAHD 120
           F LPWAQ+A SKSAA DR+ HMVHIFKPV+AALLSLPMPTIAAVSGHAAAAGF LAL+HD
Sbjct: 61  FHLPWAQSAASKSAARDRILHMVHIFKPVLAALLSLPMPTIAAVSGHAAAAGFALALSHD 120

Query: 121 FVLMRRDKGVLYMSEVDLGLTMPDYFAALMRSKIGSASVRRDVLLTGMKVNGESAVKLGI 180
           +VLMRRDKGVLYMSE+DLG T+PDYFAALMRSKIGSASVRRDVLL GMKVNGE+AV+LGI
Sbjct: 121 YVLMRRDKGVLYMSEIDLGFTLPDYFAALMRSKIGSASVRRDVLLAGMKVNGETAVELGI 180

Query: 181 ADAAHDGEEGLMEAAVAMGQKLAKRKWEGGAYAQIRKSLYPEMTPLLDGLNGKI 235
            DAAHDG E LMEAAVAMG+KLAKRKWEG AYA+IRKSLYPEM+PLL+ L+GK+
Sbjct: 181 TDAAHDGGEALMEAAVAMGEKLAKRKWEGEAYAEIRKSLYPEMSPLLNELSGKL 234

[Close]

BLAST of Moc05g00660 vs. ExPASy Swiss-Prot
Match: O23299 (Enoyl-CoA delta isomerase 2, peroxisomal OS=Arabidopsis thaliana OX=3702 GN=ECI2 PE=1 SV=1)

HSP 1 Score: 309.3 bits (791), Expect = 7.1e-83
Identity = 153/239 (64.02%), Postives = 197/239 (82.43%), Query Frame = 0


Query: 1   MCTLEKRGDVFILTLTGDNEHWIGPPFVSSLLSLLSQVKSQATRGSVLLTTSHGRFFSNG 60
           MCTLEKRGD+F+LTLTGD EH   P  ++++LSLL Q KSQ+TRGS+L+TT++G+FFSNG
Sbjct: 1   MCTLEKRGDLFLLTLTGDGEHRFHPDTIATILSLLEQAKSQSTRGSILITTANGKFFSNG 60

Query: 61  FDLPWAQAAGSKSAATDRLAHMVHIFKPVVAALLSLPMPTIAAVSGHAAAAGFLLALAHD 120
           FDL WAQ AGSK+ A +RL  MV  FKPVVAALL LPMPTIAA++GHAAAAG +LAL+HD
Sbjct: 61  FDLAWAQTAGSKTGAANRLHQMVESFKPVVAALLDLPMPTIAALNGHAAAAGLILALSHD 120

Query: 121 FVLMRRDKGVLYMSEVDLGLTMPDYFAALMRSKIGSASVRRDVLLTGMKVNGESAVKLGI 180
           +V MR+D+GVLYMSEVD+GL+MPDYF+AL+R+KIG+++ RR++LL+G K+ GE AV LGI
Sbjct: 121 YVFMRKDRGVLYMSEVDIGLSMPDYFSALVRAKIGTSAARRELLLSGKKIRGEEAVGLGI 180

Query: 181 AD-AAHDGEEGLMEAAVAMGQKLAKRKWEGGAYAQIRKSLYPEMTPLLDGLNGKIEYRP 239
            D AA+D EEG++ A+V +G+KLA +KW G  YA IRKSLYPE+  +L GL  ++   P
Sbjct: 181 VDSAAYDSEEGVVVASVRLGEKLAAKKWSGEVYASIRKSLYPELCGIL-GLETRVFATP 238

[Close]

BLAST of Moc05g00660 vs. ExPASy Swiss-Prot
Match: O23300 (Enoyl-CoA delta isomerase 3 OS=Arabidopsis thaliana OX=3702 GN=ECI3 PE=1 SV=1)

HSP 1 Score: 289.3 bits (739), Expect = 7.6e-77
Identity = 143/229 (62.45%), Postives = 184/229 (80.35%), Query Frame = 0


Query: 1   MCTLEKRGDVFILTLTGDNEHWIGPPFVSSLLSLLSQVKSQATRGSVLLTTSHGRFFSNG 60
           MCTLEKRGD+F+LTLTG++EH   P  ++S+LSLL Q KSQ+T+GSVL+TT HG+FFSNG
Sbjct: 1   MCTLEKRGDLFLLTLTGEDEHRFHPDTIASVLSLLEQAKSQSTKGSVLITTGHGKFFSNG 60

Query: 61  FDLPWAQAAGSKSAATDRLAHMVHIFKPVVAALLSLPMPTIAAVSGHAAAAGFLLALAHD 120
           FDL WAQ+AG    A  R+  MV  FKPV+AALL LPMPTIAA++GHAAA+G + AL+HD
Sbjct: 61  FDLAWAQSAG--HGAIKRMHQMVKSFKPVLAALLDLPMPTIAALNGHAAASGLMFALSHD 120

Query: 121 FVLMRRDKGVLYMSEVDLGLTMPDYFAALMRSKIGSASVRRDVLLTGMKVNGESAVKLGI 180
           +V MR+D+GVLYMSEVD+GL +PDYF+AL+ +K+GS   RR++LL+G K+ GE AV LGI
Sbjct: 121 YVFMRKDRGVLYMSEVDIGLPVPDYFSALVVAKVGSGIARRELLLSGKKLKGEEAVALGI 180

Query: 181 AD-AAHDGEEGLMEAAVAMGQKLAKRKWEGGAYAQIRKSLYPEMTPLLD 229
            D AAHD  EG++EA V++G+ LA +KW G  YA IRKSLYPE+  ++D
Sbjct: 181 VDSAAHDSAEGVVEATVSLGESLAAKKWNGEVYATIRKSLYPELCRMVD 227

[Close]

BLAST of Moc05g00660 vs. ExPASy Swiss-Prot
Match: O04469 (Enoyl-CoA delta isomerase 1, peroxisomal OS=Arabidopsis thaliana OX=3702 GN=ECI1 PE=1 SV=1)

HSP 1 Score: 206.1 bits (523), Expect = 8.5e-52
Identity = 109/224 (48.66%), Postives = 157/224 (70.09%), Query Frame = 0


Query: 1   MCTLEKRGDVFILTLTGDNEHWIGPPFVSSLLSLLSQVKSQAT-RGSVLLTTSHGRFFSN 60
           MC+LEKR  +FIL LTGD EH + P  + SL S ++Q++S  +   SVL+TTS G+FFSN
Sbjct: 1   MCSLEKRDRLFILKLTGDGEHRLNPTLLDSLRSTINQIRSDPSFSQSVLITTSDGKFFSN 60

Query: 61  GFDLPWAQAAGSKSAATDRLAHMVHIFKPVVAALLSLPMPTIAAVSGHAAAAGFLLALAH 120
           G+DL  A++  S S   D         + +VA L+SLPMPTIAAV+GHA+AAG +LA++H
Sbjct: 61  GYDLALAESNPSLSVVMDAK------LRSLVADLISLPMPTIAAVTGHASAAGCILAMSH 120

Query: 121 DFVLMRRDKGVLYMSEVDLGLTMPDYFAALMRSKIGSASVRRDVLLTGMKVNGESAVKLG 180
           D+VLMRRD+G LYMSE+D+ L +P +F A++R KIGS + RRDV+LT  KV  +  VK+G
Sbjct: 121 DYVLMRRDRGFLYMSELDIELIVPAWFMAVIRGKIGSPAARRDVMLTAAKVTADVGVKMG 180

Query: 181 IADAAHDGEEGLMEAAVAMGQKLAKRKWEGGAYAQIRKSLYPEM 224
           I D+A+      +EAA+ +G+++ +R  +G  Y ++R+SL  E+
Sbjct: 181 IVDSAYGSAAETVEAAIKLGEEIVQRGGDGHVYGKMRESLLREV 218

[Close]

BLAST of Moc05g00660 vs. ExPASy Swiss-Prot
Match: Q8XA35 (Carnitinyl-CoA dehydratase OS=Escherichia coli O157:H7 OX=83334 GN=caiD PE=3 SV=3)

HSP 1 Score: 55.8 bits (133), Expect = 1.4e-06
Identity = 49/157 (31.21%), Postives = 78/157 (49.68%), Query Frame = 0


Query: 48  LLTTSHGRFFSNGFDLPWAQAAGSKSA--ATDRLAHMVHIFKPVVAALLSLPMPTIAAVS 107
           ++T +  +FFS G+DL  A    +  A       A +  IF        +L  P IAAV+
Sbjct: 51  IITGAGEKFFSAGWDLKAAAEGEAPDADFGPGGFAGLTEIF--------NLDKPVIAAVN 110

Query: 108 GHAAAAGFLLALAHDFVLMRRDKGVLYMSEVDLGLTMPDYFAALMRSKIGSASVRRDVLL 167
           G+A   GF LALA DF++   D     + E  LG+ +PD    L   KI   ++  ++++
Sbjct: 111 GYAFGGGFELALAADFIVC-ADNASFALPEAKLGI-VPDSGGVLRLPKILPPAIVNEMVM 170

Query: 168 TGMKVNGESAVKLGIADAAHDGEEGLMEAAVAMGQKL 203
           TG ++  E A++ GI +      E LM+ A  + Q+L
Sbjct: 171 TGRRMGAEEALRWGIVNRVVSQAE-LMDNARELAQQL 196

[Close]

BLAST of Moc05g00660 vs. ExPASy Swiss-Prot
Match: B1IRE0 (Carnitinyl-CoA dehydratase OS=Escherichia coli (strain ATCC 8739 / DSM 1576 / NBRC 3972 / NCIMB 8545 / WDCM 00012 / Crooks) OX=481805 GN=caiD PE=3 SV=1)

HSP 1 Score: 55.8 bits (133), Expect = 1.4e-06
Identity = 49/157 (31.21%), Postives = 78/157 (49.68%), Query Frame = 0


Query: 48  LLTTSHGRFFSNGFDLPWAQAAGSKSA--ATDRLAHMVHIFKPVVAALLSLPMPTIAAVS 107
           ++T +  +FFS G+DL  A    +  A       A +  IF        +L  P IAAV+
Sbjct: 51  IITGAGEKFFSAGWDLKAAAEGEAPDADFGPGGFAGLTEIF--------NLDKPVIAAVN 110

Query: 108 GHAAAAGFLLALAHDFVLMRRDKGVLYMSEVDLGLTMPDYFAALMRSKIGSASVRRDVLL 167
           G+A   GF LALA DF++   D     + E  LG+ +PD    L   KI   ++  ++++
Sbjct: 111 GYAFGGGFELALAADFIVC-ADNASFALPEAKLGI-VPDSGGVLRLPKILPPAIVNEMVM 170

Query: 168 TGMKVNGESAVKLGIADAAHDGEEGLMEAAVAMGQKL 203
           TG ++  E A++ GI +      E LM+ A  + Q+L
Sbjct: 171 TGRRMGAEEALRWGIVNRVVSQAE-LMDNARELAQQL 196

[Close]

BLAST of Moc05g00660 vs. ExPASy TrEMBL
Match: A0A6J1CRE1 (enoyl-CoA delta isomerase 2, peroxisomal-like OS=Momordica charantia OX=3673 GN=LOC111013487 PE=4 SV=1)

HSP 1 Score: 456.8 bits (1174), Expect = 1.0e-124
Identity = 233/234 (99.57%), Postives = 234/234 (100.00%), Query Frame = 0


Query: 1   MCTLEKRGDVFILTLTGDNEHWIGPPFVSSLLSLLSQVKSQATRGSVLLTTSHGRFFSNG 60
           MCTLEKRGDVFILTLTGDNEHWIGPPFVSSLLSLLSQVKSQATRGSVLLTTSHGRFFSNG
Sbjct: 25  MCTLEKRGDVFILTLTGDNEHWIGPPFVSSLLSLLSQVKSQATRGSVLLTTSHGRFFSNG 84

Query: 61  FDLPWAQAAGSKSAATDRLAHMVHIFKPVVAALLSLPMPTIAAVSGHAAAAGFLLALAHD 120
           FDLPWAQAAGSKSAATDRLAHMVHIFKPVVAALLSLPMPTIAAVSGHAAAAGFLLALAHD
Sbjct: 85  FDLPWAQAAGSKSAATDRLAHMVHIFKPVVAALLSLPMPTIAAVSGHAAAAGFLLALAHD 144

Query: 121 FVLMRRDKGVLYMSEVDLGLTMPDYFAALMRSKIGSASVRRDVLLTGMKVNGESAVKLGI 180
           FVLMRRDKGVLYMSEVDLGLTMPDYFAALMRSKIGSASVRRDVLLTGMKVNGESAVKLGI
Sbjct: 145 FVLMRRDKGVLYMSEVDLGLTMPDYFAALMRSKIGSASVRRDVLLTGMKVNGESAVKLGI 204

Query: 181 ADAAHDGEEGLMEAAVAMGQKLAKRKWEGGAYAQIRKSLYPEMTPLLDGLNGKI 235
           ADAAHDGEEGLMEAAVAMGQKLAKRKWEGGAYAQIRKSLYPEMTPLLDGLNGK+
Sbjct: 205 ADAAHDGEEGLMEAAVAMGQKLAKRKWEGGAYAQIRKSLYPEMTPLLDGLNGKM 258

[Close]

BLAST of Moc05g00660 vs. ExPASy TrEMBL
Match: A0A6J1IDW4 (enoyl-CoA delta isomerase 2, peroxisomal-like OS=Cucurbita maxima OX=3661 GN=LOC111474523 PE=4 SV=1)

HSP 1 Score: 401.0 bits (1029), Expect = 6.7e-108
Identity = 197/234 (84.19%), Postives = 220/234 (94.02%), Query Frame = 0


Query: 1   MCTLEKRGDVFILTLTGDNEHWIGPPFVSSLLSLLSQVKSQATRGSVLLTTSHGRFFSNG 60
           MCTLE+RG++FI+TLTGD+EHWIGPPF+SS LSLL+Q+ SQAT GSVL+TTSHGRFFSNG
Sbjct: 1   MCTLERRGNLFIITLTGDDEHWIGPPFISSFLSLLTQINSQATPGSVLITTSHGRFFSNG 60

Query: 61  FDLPWAQAAGSKSAATDRLAHMVHIFKPVVAALLSLPMPTIAAVSGHAAAAGFLLALAHD 120
           F LPWAQ+A SKSAA DR+ HMVHIFKPV+AALLSLPMPTIAAVSGHAAAAGF LAL+HD
Sbjct: 61  FHLPWAQSAASKSAARDRILHMVHIFKPVLAALLSLPMPTIAAVSGHAAAAGFALALSHD 120

Query: 121 FVLMRRDKGVLYMSEVDLGLTMPDYFAALMRSKIGSASVRRDVLLTGMKVNGESAVKLGI 180
           +VLMRRDKGVLYMSE+DLG T+PDYFAALMRSKIGSASVRRDVLL GMKVNGE+AVKLGI
Sbjct: 121 YVLMRRDKGVLYMSEIDLGFTLPDYFAALMRSKIGSASVRRDVLLAGMKVNGETAVKLGI 180

Query: 181 ADAAHDGEEGLMEAAVAMGQKLAKRKWEGGAYAQIRKSLYPEMTPLLDGLNGKI 235
            DAAHDGEE LMEAAVAMG+KLAKRKWEG AYA+IRKSLYPEM+PLL+ L+GK+
Sbjct: 181 TDAAHDGEEALMEAAVAMGEKLAKRKWEGEAYAEIRKSLYPEMSPLLNELSGKL 234

[Close]

BLAST of Moc05g00660 vs. ExPASy TrEMBL
Match: A0A6J1FNU4 (enoyl-CoA delta isomerase 2, peroxisomal-like OS=Cucurbita moschata OX=3662 GN=LOC111447470 PE=4 SV=1)

HSP 1 Score: 398.7 bits (1023), Expect = 3.3e-107
Identity = 196/234 (83.76%), Postives = 219/234 (93.59%), Query Frame = 0


Query: 1   MCTLEKRGDVFILTLTGDNEHWIGPPFVSSLLSLLSQVKSQATRGSVLLTTSHGRFFSNG 60
           MCTLE+RGD+FI+TLTGD+EHWIGPPF+SS LSLL+Q+ SQAT GSVL+TTSHGRFFSNG
Sbjct: 1   MCTLERRGDLFIITLTGDDEHWIGPPFISSFLSLLTQINSQATPGSVLITTSHGRFFSNG 60

Query: 61  FDLPWAQAAGSKSAATDRLAHMVHIFKPVVAALLSLPMPTIAAVSGHAAAAGFLLALAHD 120
           F LPWAQ+A SKSAA DR+ HMVHIFKPV+AALLSLPMPTIAAVSGHAAAAGF LAL+HD
Sbjct: 61  FHLPWAQSAASKSAARDRILHMVHIFKPVLAALLSLPMPTIAAVSGHAAAAGFALALSHD 120

Query: 121 FVLMRRDKGVLYMSEVDLGLTMPDYFAALMRSKIGSASVRRDVLLTGMKVNGESAVKLGI 180
           +VLMRRDKGVLYMSE+DLG T+PDYFAALMRSKIGSASVRRDVLL GMKVNGE+AV+LGI
Sbjct: 121 YVLMRRDKGVLYMSEIDLGFTLPDYFAALMRSKIGSASVRRDVLLAGMKVNGETAVELGI 180

Query: 181 ADAAHDGEEGLMEAAVAMGQKLAKRKWEGGAYAQIRKSLYPEMTPLLDGLNGKI 235
            DAAHDG E LMEAAVAMG+KLAKRKWEG AYA+IRKSLYPEM+PLL+ L+GK+
Sbjct: 181 TDAAHDGGEALMEAAVAMGEKLAKRKWEGEAYAEIRKSLYPEMSPLLNELSGKL 234

[Close]

BLAST of Moc05g00660 vs. ExPASy TrEMBL
Match: A0A1S3AXB9 (enoyl-CoA delta isomerase 2, peroxisomal-like OS=Cucumis melo OX=3656 GN=LOC103483621 PE=4 SV=1)

HSP 1 Score: 390.2 bits (1001), Expect = 1.2e-104
Identity = 191/234 (81.62%), Postives = 212/234 (90.60%), Query Frame = 0


Query: 1   MCTLEKRGDVFILTLTGDNEHWIGPPFVSSLLSLLSQVKSQATRGSVLLTTSHGRFFSNG 60
           MCTLEKRG +FILTLTGDNEHWIGP F+S+ +SLL+Q+KSQATRGSVLLTTSHGRFFSNG
Sbjct: 1   MCTLEKRGSLFILTLTGDNEHWIGPTFISTFISLLAQIKSQATRGSVLLTTSHGRFFSNG 60

Query: 61  FDLPWAQAAGSKSAATDRLAHMVHIFKPVVAALLSLPMPTIAAVSGHAAAAGFLLALAHD 120
           FDLPWAQ A SKSAA DR+ HMVHIFKP+VAALLSLPMPTIAA+SGHAAAAGF LAL HD
Sbjct: 61  FDLPWAQTAPSKSAARDRIVHMVHIFKPIVAALLSLPMPTIAAISGHAAAAGFALALCHD 120

Query: 121 FVLMRRDKGVLYMSEVDLGLTMPDYFAALMRSKIGSASVRRDVLLTGMKVNGESAVKLGI 180
           +VLMRRDKGV+YMSE+DLGLTMPDYFAAL+RSKI S SVRRDVLL G KVNGE+A KLGI
Sbjct: 121 YVLMRRDKGVIYMSEIDLGLTMPDYFAALVRSKISSVSVRRDVLLAGRKVNGETAAKLGI 180

Query: 181 ADAAHDGEEGLMEAAVAMGQKLAKRKWEGGAYAQIRKSLYPEMTPLLDGLNGKI 235
            D+ HD EE LMEAAVAMG+ LA+RKWE  AYA+IRKSLYPEM+PLL+ LNGK+
Sbjct: 181 VDSVHDNEEALMEAAVAMGEMLARRKWESEAYAEIRKSLYPEMSPLLNELNGKM 234

[Close]

BLAST of Moc05g00660 vs. ExPASy TrEMBL
Match: A0A5A7U4L1 (Enoyl-CoA delta isomerase 2, peroxisomal-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold434G001340 PE=4 SV=1)

HSP 1 Score: 389.8 bits (1000), Expect = 1.5e-104
Identity = 191/234 (81.62%), Postives = 212/234 (90.60%), Query Frame = 0


Query: 1   MCTLEKRGDVFILTLTGDNEHWIGPPFVSSLLSLLSQVKSQATRGSVLLTTSHGRFFSNG 60
           MCTLEKRG +FILTLTGDNEHWIGP F+S+ +SLL+Q+KSQATRGSVLLTTSHGRFFSNG
Sbjct: 1   MCTLEKRGSLFILTLTGDNEHWIGPTFISTFISLLAQIKSQATRGSVLLTTSHGRFFSNG 60

Query: 61  FDLPWAQAAGSKSAATDRLAHMVHIFKPVVAALLSLPMPTIAAVSGHAAAAGFLLALAHD 120
           FDLPWAQ A SKSAA DR+ HMVHIFKP+VAALLSLPMPTIAA+SGHAAAAGF LAL HD
Sbjct: 61  FDLPWAQTAPSKSAARDRIVHMVHIFKPIVAALLSLPMPTIAAISGHAAAAGFALALCHD 120

Query: 121 FVLMRRDKGVLYMSEVDLGLTMPDYFAALMRSKIGSASVRRDVLLTGMKVNGESAVKLGI 180
           +VLMRRDKGV+YMSE+DLGLTMPDYFAAL+RSKI S SVRRDVLL G KVNGE+A KLGI
Sbjct: 121 YVLMRRDKGVIYMSEIDLGLTMPDYFAALVRSKISSVSVRRDVLLAGRKVNGETAAKLGI 180

Query: 181 ADAAHDGEEGLMEAAVAMGQKLAKRKWEGGAYAQIRKSLYPEMTPLLDGLNGKI 235
            D+ HD EE LMEAAVAMG+ LA+RKWE  AYA+IRKSLYPEM+PLL+ LNGK+
Sbjct: 181 IDSVHDNEEALMEAAVAMGEMLARRKWESEAYAEIRKSLYPEMSPLLNELNGKM 234

[Close]

BLAST of Moc05g00660 vs. TAIR 10
Match: AT4G14430.1 (indole-3-butyric acid response 10 )

HSP 1 Score: 309.3 bits (791), Expect = 5.1e-84
Identity = 153/239 (64.02%), Postives = 197/239 (82.43%), Query Frame = 0


Query: 1   MCTLEKRGDVFILTLTGDNEHWIGPPFVSSLLSLLSQVKSQATRGSVLLTTSHGRFFSNG 60
           MCTLEKRGD+F+LTLTGD EH   P  ++++LSLL Q KSQ+TRGS+L+TT++G+FFSNG
Sbjct: 1   MCTLEKRGDLFLLTLTGDGEHRFHPDTIATILSLLEQAKSQSTRGSILITTANGKFFSNG 60

Query: 61  FDLPWAQAAGSKSAATDRLAHMVHIFKPVVAALLSLPMPTIAAVSGHAAAAGFLLALAHD 120
           FDL WAQ AGSK+ A +RL  MV  FKPVVAALL LPMPTIAA++GHAAAAG +LAL+HD
Sbjct: 61  FDLAWAQTAGSKTGAANRLHQMVESFKPVVAALLDLPMPTIAALNGHAAAAGLILALSHD 120

Query: 121 FVLMRRDKGVLYMSEVDLGLTMPDYFAALMRSKIGSASVRRDVLLTGMKVNGESAVKLGI 180
           +V MR+D+GVLYMSEVD+GL+MPDYF+AL+R+KIG+++ RR++LL+G K+ GE AV LGI
Sbjct: 121 YVFMRKDRGVLYMSEVDIGLSMPDYFSALVRAKIGTSAARRELLLSGKKIRGEEAVGLGI 180

Query: 181 AD-AAHDGEEGLMEAAVAMGQKLAKRKWEGGAYAQIRKSLYPEMTPLLDGLNGKIEYRP 239
            D AA+D EEG++ A+V +G+KLA +KW G  YA IRKSLYPE+  +L GL  ++   P
Sbjct: 181 VDSAAYDSEEGVVVASVRLGEKLAAKKWSGEVYASIRKSLYPELCGIL-GLETRVFATP 238

[Close]

BLAST of Moc05g00660 vs. TAIR 10
Match: AT4G14440.1 (3-hydroxyacyl-CoA dehydratase 1 )

HSP 1 Score: 289.3 bits (739), Expect = 5.4e-78
Identity = 143/229 (62.45%), Postives = 184/229 (80.35%), Query Frame = 0


Query: 1   MCTLEKRGDVFILTLTGDNEHWIGPPFVSSLLSLLSQVKSQATRGSVLLTTSHGRFFSNG 60
           MCTLEKRGD+F+LTLTG++EH   P  ++S+LSLL Q KSQ+T+GSVL+TT HG+FFSNG
Sbjct: 1   MCTLEKRGDLFLLTLTGEDEHRFHPDTIASVLSLLEQAKSQSTKGSVLITTGHGKFFSNG 60

Query: 61  FDLPWAQAAGSKSAATDRLAHMVHIFKPVVAALLSLPMPTIAAVSGHAAAAGFLLALAHD 120
           FDL WAQ+AG    A  R+  MV  FKPV+AALL LPMPTIAA++GHAAA+G + AL+HD
Sbjct: 61  FDLAWAQSAG--HGAIKRMHQMVKSFKPVLAALLDLPMPTIAALNGHAAASGLMFALSHD 120

Query: 121 FVLMRRDKGVLYMSEVDLGLTMPDYFAALMRSKIGSASVRRDVLLTGMKVNGESAVKLGI 180
           +V MR+D+GVLYMSEVD+GL +PDYF+AL+ +K+GS   RR++LL+G K+ GE AV LGI
Sbjct: 121 YVFMRKDRGVLYMSEVDIGLPVPDYFSALVVAKVGSGIARRELLLSGKKLKGEEAVALGI 180

Query: 181 AD-AAHDGEEGLMEAAVAMGQKLAKRKWEGGAYAQIRKSLYPEMTPLLD 229
            D AAHD  EG++EA V++G+ LA +KW G  YA IRKSLYPE+  ++D
Sbjct: 181 VDSAAHDSAEGVVEATVSLGESLAAKKWNGEVYATIRKSLYPELCRMVD 227

[Close]

BLAST of Moc05g00660 vs. TAIR 10
Match: AT1G65520.1 (delta(3), delta(2)-enoyl CoA isomerase 1 )

HSP 1 Score: 206.1 bits (523), Expect = 6.0e-53
Identity = 109/224 (48.66%), Postives = 157/224 (70.09%), Query Frame = 0


Query: 1   MCTLEKRGDVFILTLTGDNEHWIGPPFVSSLLSLLSQVKSQAT-RGSVLLTTSHGRFFSN 60
           MC+LEKR  +FIL LTGD EH + P  + SL S ++Q++S  +   SVL+TTS G+FFSN
Sbjct: 1   MCSLEKRDRLFILKLTGDGEHRLNPTLLDSLRSTINQIRSDPSFSQSVLITTSDGKFFSN 60

Query: 61  GFDLPWAQAAGSKSAATDRLAHMVHIFKPVVAALLSLPMPTIAAVSGHAAAAGFLLALAH 120
           G+DL  A++  S S   D         + +VA L+SLPMPTIAAV+GHA+AAG +LA++H
Sbjct: 61  GYDLALAESNPSLSVVMDAK------LRSLVADLISLPMPTIAAVTGHASAAGCILAMSH 120

Query: 121 DFVLMRRDKGVLYMSEVDLGLTMPDYFAALMRSKIGSASVRRDVLLTGMKVNGESAVKLG 180
           D+VLMRRD+G LYMSE+D+ L +P +F A++R KIGS + RRDV+LT  KV  +  VK+G
Sbjct: 121 DYVLMRRDRGFLYMSELDIELIVPAWFMAVIRGKIGSPAARRDVMLTAAKVTADVGVKMG 180

Query: 181 IADAAHDGEEGLMEAAVAMGQKLAKRKWEGGAYAQIRKSLYPEM 224
           I D+A+      +EAA+ +G+++ +R  +G  Y ++R+SL  E+
Sbjct: 181 IVDSAYGSAAETVEAAIKLGEEIVQRGGDGHVYGKMRESLLREV 218

The following BLAST results are available for this feature:

BLAST of Moc05g00660 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG7018780.1	1.0e-147	62.92	Enoyl-CoA delta isomerase 3, partial [Cucurbita argyrosperma subsp. argyrosperma...	[more]
XP_022143632.1	2.1e-124	99.57	enoyl-CoA delta isomerase 2, peroxisomal-like [Momordica charantia]	[more]
KAF9677702.1	4.4e-122	57.53	hypothetical protein SADUNF_Sadunf08G0135000 [Salix dunnii]	[more]
XP_022975055.1	1.4e-107	84.19	enoyl-CoA delta isomerase 2, peroxisomal-like [Cucurbita maxima] >XP_022975341.1...	[more]
XP_022942426.1	6.8e-107	83.76	enoyl-CoA delta isomerase 2, peroxisomal-like [Cucurbita moschata]	[more]

BLAST of Moc05g00660 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
O23299	7.1e-83	64.02	Enoyl-CoA delta isomerase 2, peroxisomal OS=Arabidopsis thaliana OX=3702 GN=ECI2...	[more]
O23300	7.6e-77	62.45	Enoyl-CoA delta isomerase 3 OS=Arabidopsis thaliana OX=3702 GN=ECI3 PE=1 SV=1	[more]
O04469	8.5e-52	48.66	Enoyl-CoA delta isomerase 1, peroxisomal OS=Arabidopsis thaliana OX=3702 GN=ECI1...	[more]
Q8XA35	1.4e-06	31.21	Carnitinyl-CoA dehydratase OS=Escherichia coli O157:H7 OX=83334 GN=caiD PE=3 SV=...	[more]
B1IRE0	1.4e-06	31.21	Carnitinyl-CoA dehydratase OS=Escherichia coli (strain ATCC 8739 / DSM 1576 / NB...	[more]

BLAST of Moc05g00660 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1CRE1	1.0e-124	99.57	enoyl-CoA delta isomerase 2, peroxisomal-like OS=Momordica charantia OX=3673 GN=...	[more]
A0A6J1IDW4	6.7e-108	84.19	enoyl-CoA delta isomerase 2, peroxisomal-like OS=Cucurbita maxima OX=3661 GN=LOC...	[more]
A0A6J1FNU4	3.3e-107	83.76	enoyl-CoA delta isomerase 2, peroxisomal-like OS=Cucurbita moschata OX=3662 GN=L...	[more]
A0A1S3AXB9	1.2e-104	81.62	enoyl-CoA delta isomerase 2, peroxisomal-like OS=Cucumis melo OX=3656 GN=LOC1034...	[more]
A0A5A7U4L1	1.5e-104	81.62	Enoyl-CoA delta isomerase 2, peroxisomal-like OS=Cucumis melo var. makuwa OX=119...	[more]

BLAST of Moc05g00660 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G14430.1	5.1e-84	64.02	indole-3-butyric acid response 10	[more]
AT4G14440.1	5.4e-78	62.45	3-hydroxyacyl-CoA dehydratase 1	[more]
AT1G65520.1	6.0e-53	48.66	delta(3), delta(2)-enoyl CoA isomerase 1	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (OHB3-1) v2
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001753	Enoyl-CoA hydratase/isomerase	PFAM	PF00378	ECH_1	coord: 28..206 e-value: 5.9E-21 score: 74.9 coord: 265..384 e-value: 4.3E-11 score: 42.6
None	No IPR available	GENE3D	3.90.226.10		coord: 1..230 e-value: 4.3E-38 score: 132.9 coord: 242..409 e-value: 2.1E-22 score: 81.5
None	No IPR available	PANTHER	PTHR11941	ENOYL-COA HYDRATASE-RELATED	coord: 1..228
None	No IPR available	PANTHER	PTHR11941:SF75	ENOYL-COA DELTA ISOMERASE 2, PEROXISOMAL	coord: 244..393 coord: 1..228
None	No IPR available	PANTHER	PTHR11941	ENOYL-COA HYDRATASE-RELATED	coord: 244..393
None	No IPR available	CDD	cd06558	crotonase-like	coord: 2..202 e-value: 4.32821E-15 score: 71.4376
None	No IPR available	CDD	cd06558	crotonase-like	coord: 244..381 e-value: 3.89583E-22 score: 91.468
IPR029045	ClpP/crotonase-like domain superfamily	SUPERFAMILY	52096	ClpP/crotonase	coord: 3..206
IPR029045	ClpP/crotonase-like domain superfamily	SUPERFAMILY	52096	ClpP/crotonase	coord: 244..392

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Moc05g00660.1	Moc05g00660.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006635	fatty acid beta-oxidation
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0004165	dodecenoyl-CoA delta-isomerase activity
molecular_function	GO:0004300	enoyl-CoA hydratase activity
molecular_function	GO:0003824	catalytic activity