Moc04g39760 (gene) Bitter gourd (OHB3-1) v2

Overview
NameMoc04g39760
Typegene
OrganismMomordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
Descriptionalpha/beta-Hydrolases superfamily protein
Locationchr4: 29489023 .. 29490060 (-)
RNA-Seq ExpressionMoc04g39760
SyntenyMoc04g39760
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGACTTCCACCGGCCACACACAACCTCCATCTTCCTCCGCTCCCACCCTTCCATGGAAGCGTAAGCTTGCCAATCTTCTCTCCAGCTTCCTTTTCAGACGGATTTTCCGATCAGATTTCACGATCAACCGCCGCCTCCTCCGCCTTTTAGACCGCAAAGTACCTCCTTCTTCCACACCGCTGCACGGCGTCGCCTCCTTTGACACCGCCGCCTTTGCTTCCTCCCCCGACCTCTGGGCTCGTGTTTATATCCCTGTCACCTTCGCCGCCGCCGATCGTTTGCCGGTAATCGTATTCTTCCATGGCGGAGGCTTCGTTTTATTCTCCACCGACACTCCACAGGTTGACTCGTTTTCACTCGATTGCGCTCGCGAGGTTGGAGCCATCGTCGTCTCCGTCAACTACCGCCTCGCTCCGGAGCACCAATTTCCGAGCCAGTACGACGACGGATTCCACGTTCTCAAGGCAATCGACGAAGGGTTGATTTCCGAAACCCTTCCTCAGAACGCCGACCTGAGCCGCTGTTTCTTGGCAGGGGAGAGTGCCGGAGGAAACATAGCGCACCACGTGGCGGTGAGAGCTGCGGAGGATCGTCAATTCAAGAGGCTCAGAATCGCAGGCCTGATTGCAATTCAGCCATTTTTCGGAGGAGAAGAGCGGACCGAATCAGAGCTTCGATTAGGGAACGGTTCCGAATCCCGACTGAGTCTGGAATTGACGGATTGGTTTTGGAAGGCATATTTGCCGGCCGGGTGTGACCGGGAGCATCCAGCGGCGAACGTGGTCGTCGGATCGGACATTTCTGGCCTGGATTTTCCACCGACGCTACTTGTGATCGGAGGTTTGGATCCGTTGAAGGATCGGAACAAGGAGTACGGTGAGTGGTTGGAGAAATCGGGTAAGGAAGTTCGGGTCGTGGAGTATCCGAATGGGATCCATGGGTCGACTTGGAACCCGGATCTTCCAGAATATTCTACGCTGATGCAGGACACCAGGGAATTCATGGAGCATCAGCATAGCAAGCAGCTGAATTTCTAA

mRNA sequence

ATGACTTCCACCGGCCACACACAACCTCCATCTTCCTCCGCTCCCACCCTTCCATGGAAGCGTAAGCTTGCCAATCTTCTCTCCAGCTTCCTTTTCAGACGGATTTTCCGATCAGATTTCACGATCAACCGCCGCCTCCTCCGCCTTTTAGACCGCAAAGTACCTCCTTCTTCCACACCGCTGCACGGCGTCGCCTCCTTTGACACCGCCGCCTTTGCTTCCTCCCCCGACCTCTGGGCTCGTGTTTATATCCCTGTCACCTTCGCCGCCGCCGATCGTTTGCCGGTAATCGTATTCTTCCATGGCGGAGGCTTCGTTTTATTCTCCACCGACACTCCACAGGTTGACTCGTTTTCACTCGATTGCGCTCGCGAGGTTGGAGCCATCGTCGTCTCCGTCAACTACCGCCTCGCTCCGGAGCACCAATTTCCGAGCCAGTACGACGACGGATTCCACGTTCTCAAGGCAATCGACGAAGGGTTGATTTCCGAAACCCTTCCTCAGAACGCCGACCTGAGCCGCTGTTTCTTGGCAGGGGAGAGTGCCGGAGGAAACATAGCGCACCACGTGGCGGTGAGAGCTGCGGAGGATCGTCAATTCAAGAGGCTCAGAATCGCAGGCCTGATTGCAATTCAGCCATTTTTCGGAGGAGAAGAGCGGACCGAATCAGAGCTTCGATTAGGGAACGGTTCCGAATCCCGACTGAGTCTGGAATTGACGGATTGGTTTTGGAAGGCATATTTGCCGGCCGGGTGTGACCGGGAGCATCCAGCGGCGAACGTGGTCGTCGGATCGGACATTTCTGGCCTGGATTTTCCACCGACGCTACTTGTGATCGGAGGTTTGGATCCGTTGAAGGATCGGAACAAGGAGTACGGTGAGTGGTTGGAGAAATCGGGTAAGGAAGTTCGGGTCGTGGAGTATCCGAATGGGATCCATGGGTCGACTTGGAACCCGGATCTTCCAGAATATTCTACGCTGATGCAGGACACCAGGGAATTCATGGAGCATCAGCATAGCAAGCAGCTGAATTTCTAA

Coding sequence (CDS)

ATGACTTCCACCGGCCACACACAACCTCCATCTTCCTCCGCTCCCACCCTTCCATGGAAGCGTAAGCTTGCCAATCTTCTCTCCAGCTTCCTTTTCAGACGGATTTTCCGATCAGATTTCACGATCAACCGCCGCCTCCTCCGCCTTTTAGACCGCAAAGTACCTCCTTCTTCCACACCGCTGCACGGCGTCGCCTCCTTTGACACCGCCGCCTTTGCTTCCTCCCCCGACCTCTGGGCTCGTGTTTATATCCCTGTCACCTTCGCCGCCGCCGATCGTTTGCCGGTAATCGTATTCTTCCATGGCGGAGGCTTCGTTTTATTCTCCACCGACACTCCACAGGTTGACTCGTTTTCACTCGATTGCGCTCGCGAGGTTGGAGCCATCGTCGTCTCCGTCAACTACCGCCTCGCTCCGGAGCACCAATTTCCGAGCCAGTACGACGACGGATTCCACGTTCTCAAGGCAATCGACGAAGGGTTGATTTCCGAAACCCTTCCTCAGAACGCCGACCTGAGCCGCTGTTTCTTGGCAGGGGAGAGTGCCGGAGGAAACATAGCGCACCACGTGGCGGTGAGAGCTGCGGAGGATCGTCAATTCAAGAGGCTCAGAATCGCAGGCCTGATTGCAATTCAGCCATTTTTCGGAGGAGAAGAGCGGACCGAATCAGAGCTTCGATTAGGGAACGGTTCCGAATCCCGACTGAGTCTGGAATTGACGGATTGGTTTTGGAAGGCATATTTGCCGGCCGGGTGTGACCGGGAGCATCCAGCGGCGAACGTGGTCGTCGGATCGGACATTTCTGGCCTGGATTTTCCACCGACGCTACTTGTGATCGGAGGTTTGGATCCGTTGAAGGATCGGAACAAGGAGTACGGTGAGTGGTTGGAGAAATCGGGTAAGGAAGTTCGGGTCGTGGAGTATCCGAATGGGATCCATGGGTCGACTTGGAACCCGGATCTTCCAGAATATTCTACGCTGATGCAGGACACCAGGGAATTCATGGAGCATCAGCATAGCAAGCAGCTGAATTTCTAA

Protein sequence

MTSTGHTQPPSSSAPTLPWKRKLANLLSSFLFRRIFRSDFTINRRLLRLLDRKVPPSSTPLHGVASFDTAAFASSPDLWARVYIPVTFAAADRLPVIVFFHGGGFVLFSTDTPQVDSFSLDCAREVGAIVVSVNYRLAPEHQFPSQYDDGFHVLKAIDEGLISETLPQNADLSRCFLAGESAGGNIAHHVAVRAAEDRQFKRLRIAGLIAIQPFFGGEERTESELRLGNGSESRLSLELTDWFWKAYLPAGCDREHPAANVVVGSDISGLDFPPTLLVIGGLDPLKDRNKEYGEWLEKSGKEVRVVEYPNGIHGSTWNPDLPEYSTLMQDTREFMEHQHSKQLNF
Homology
BLAST of Moc04g39760 vs. NCBI nr
Match: XP_023549493.1 (probable carboxylesterase 18 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 479.9 bits (1234), Expect = 1.8e-131
Identity = 246/345 (71.30%), Postives = 279/345 (80.87%), Query Frame = 0

Query: 1   MTSTGHTQPPSSSAPTLPWKRKLANLLSSFLFRRIFRSDFTINRRLLRLLDRKVPPSSTP 60
           M+STG TQ PSSS P LP KRKLA+LLSS LFR  FRSDFT+NR  LRLLDRKVPPSST 
Sbjct: 1   MSSTGDTQSPSSS-PNLPSKRKLADLLSSVLFRHAFRSDFTVNRGFLRLLDRKVPPSSTN 60

Query: 61  LHGVASFDTAAFASSPDLWARVYIPVTFAAADRLPVIVFFHGGGFVLFSTDTPQVDSFSL 120
             GVASFDT   +SS DLW RVY P T + +DR+PVI++FHGGGFV  S D+ Q+DSFS 
Sbjct: 61  -RGVASFDTTLDSSSSDLWLRVYNPPTCSESDRVPVIIYFHGGGFVFGSADSQQIDSFSR 120

Query: 121 DCAREVGAIVVSVNYRLAPEHQFPSQYDDGFHVLKAIDEGLISETLPQNADLSRCFLAGE 180
           D ARE+GAIVVSVNYRLAPEH+FPS YDDGF VLKAIDEG IS++LP+NAD+SRCF+AGE
Sbjct: 121 DFAREIGAIVVSVNYRLAPEHRFPSPYDDGFRVLKAIDEGSISKSLPENADISRCFIAGE 180

Query: 181 SAGGNIAHHVAVRAAEDRQFKRLRIAGLIAIQPFFGGEERTESELRLGNGSESRLSLELT 240
           SAGGNI HHV VRAAE  +FKRLRIAG+IAIQPF GGEERT+SE+R G G    L+LE+T
Sbjct: 181 SAGGNIVHHVTVRAAE-YEFKRLRIAGMIAIQPFLGGEERTKSEIRFGRG--GGLTLEVT 240

Query: 241 DWFWKAYLPAGCDREHPAANVV-VGSDISGLDFPPTLLVIGGLDPLKDRNKEYGEWLEKS 300
           DW+WKA+LP G  R+HPAANVV  GSD+SGL  PP L+V GGLD L DR KEY EWLE+S
Sbjct: 241 DWYWKAFLPIGSSRDHPAANVVRSGSDVSGLKLPPALVVNGGLDLLSDRIKEYCEWLERS 300

Query: 301 GKEVRVVEYPNGIHGSTWNPDLPEYSTLMQDTREFMEHQHSKQLN 345
           GKEVRVVEYPNGIHG +W  DLPEYS LMQD REF   +   +LN
Sbjct: 301 GKEVRVVEYPNGIHGFSWFSDLPEYSMLMQDAREFTRQKGLVKLN 340

BLAST of Moc04g39760 vs. NCBI nr
Match: XP_022990625.1 (probable carboxylesterase 18 [Cucurbita maxima])

HSP 1 Score: 475.3 bits (1222), Expect = 4.4e-130
Identity = 240/335 (71.64%), Postives = 274/335 (81.79%), Query Frame = 0

Query: 1   MTSTGHTQPPSSSAPTLPWKRKLANLLSSFLFRRIFRSDFTINRRLLRLLDRKVPPSSTP 60
           M+STG TQ PSSS P LP KRKLA+L +S LFR +FRSDFT+NR LLRLLDRKVPPS+T 
Sbjct: 1   MSSTGDTQSPSSS-PNLPSKRKLADLFTSVLFRHVFRSDFTVNRGLLRLLDRKVPPSTT- 60

Query: 61  LHGVASFDTAAFASSPDLWARVYIPVTFAAADRLPVIVFFHGGGFVLFSTDTPQVDSFSL 120
             GVASFDT   +SS DLW RVY P T + +DR+PVI++FHGGGFV  S D+ Q+DSFS 
Sbjct: 61  -RGVASFDTTLDSSSSDLWLRVYNPPTCSESDRMPVIIYFHGGGFVFGSADSQQIDSFSR 120

Query: 121 DCAREVGAIVVSVNYRLAPEHQFPSQYDDGFHVLKAIDEGLISETLPQNADLSRCFLAGE 180
           D ARE+GAIVVSVNYRLAPEH+FPS YDDGF VLKAIDEG I+++LP+NAD+SRCF+AGE
Sbjct: 121 DFAREIGAIVVSVNYRLAPEHRFPSPYDDGFRVLKAIDEGSIAKSLPENADISRCFIAGE 180

Query: 181 SAGGNIAHHVAVRAAEDRQFKRLRIAGLIAIQPFFGGEERTESELRLGNGSESRLSLELT 240
           SAGGNI HHV VRAA+  +FKRLRIAG+IAIQPF GGEERT+SE+R G G    L+LE  
Sbjct: 181 SAGGNIVHHVTVRAAK-YEFKRLRIAGMIAIQPFLGGEERTQSEIRFGRG--GGLTLETA 240

Query: 241 DWFWKAYLPAGCDREHPAANVV-VGSDISGLDFPPTLLVIGGLDPLKDRNKEYGEWLEKS 300
           DW+WKA+LP G  R+HPAANVV  GSD+SGL  PP L+V GGLD L DR KEY EWLEKS
Sbjct: 241 DWYWKAFLPIGSSRDHPAANVVRSGSDVSGLKLPPALVVNGGLDLLSDRIKEYCEWLEKS 300

Query: 301 GKEVRVVEYPNGIHGSTWNPDLPEYSTLMQDTREF 335
           GKEVRVVEYPNGIHG +W  DLPEYS LMQD REF
Sbjct: 301 GKEVRVVEYPNGIHGFSWFSDLPEYSMLMQDAREF 329

BLAST of Moc04g39760 vs. NCBI nr
Match: KAG6602724.1 (putative carboxylesterase 18, partial [Cucurbita argyrosperma subsp. sororia] >KAG7033410.1 putative carboxylesterase 18, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 474.2 bits (1219), Expect = 9.8e-130
Identity = 242/334 (72.46%), Postives = 272/334 (81.44%), Query Frame = 0

Query: 1   MTSTGHTQPPSSSAPTLPWKRKLANLLSSFLFRRIFRSDFTINRRLLRLLDRKVPPSSTP 60
           M STG TQ PSSS P LP KRKLA+LLSS LFR  FRSDFT+NR  LRLLDRKVPPSST 
Sbjct: 1   MPSTGDTQSPSSS-PNLPSKRKLADLLSSILFRHAFRSDFTVNRGFLRLLDRKVPPSST- 60

Query: 61  LHGVASFDTAAFASSPDLWARVYIPVTFAAADRLPVIVFFHGGGFVLFSTDTPQVDSFSL 120
             GVASFDT   +SS DLW RVY P+T + +DR+PVI++FHGGGFV  S D+ Q+DSFS 
Sbjct: 61  TRGVASFDTTLDSSSSDLWLRVYNPLTCSESDRMPVIIYFHGGGFVFGSADSQQIDSFSR 120

Query: 121 DCAREVGAIVVSVNYRLAPEHQFPSQYDDGFHVLKAIDEGLISETLPQNADLSRCFLAGE 180
           D ARE+GAIVVSVNY LAPEH+FPS YDDGF VLKAIDEG IS++LP+NAD+S CF+AGE
Sbjct: 121 DFAREIGAIVVSVNYCLAPEHRFPSPYDDGFRVLKAIDEGSISKSLPENADISSCFIAGE 180

Query: 181 SAGGNIAHHVAVRAAEDRQFKRLRIAGLIAIQPFFGGEERTESELRLGNGSESRLSLELT 240
           SAGGNI HHV VRAAE  +FKRLRIAG+IAIQPF GGEERT+SE+R G G    L+LE+T
Sbjct: 181 SAGGNIVHHVTVRAAE-YEFKRLRIAGMIAIQPFLGGEERTKSEIRFGRG--GGLTLEMT 240

Query: 241 DWFWKAYLPAGCDREHPAANVVVGSDISGLDFPPTLLVIGGLDPLKDRNKEYGEWLEKSG 300
           DW+WKA+LP G  R+HPAAN VV SD+SGL  PP L+V GGLD L DR KEY EWLEKSG
Sbjct: 241 DWYWKAFLPIGSSRDHPAAN-VVRSDVSGLKLPPALVVNGGLDLLSDRIKEYCEWLEKSG 300

Query: 301 KEVRVVEYPNGIHGSTWNPDLPEYSTLMQDTREF 335
           KEVRVVEYPNGIHG +W  DLPEYS LMQD REF
Sbjct: 301 KEVRVVEYPNGIHGFSWFSDLPEYSMLMQDAREF 328

BLAST of Moc04g39760 vs. NCBI nr
Match: XP_022964989.1 (probable carboxylesterase 18 [Cucurbita moschata])

HSP 1 Score: 471.5 bits (1212), Expect = 6.4e-129
Identity = 239/334 (71.56%), Postives = 272/334 (81.44%), Query Frame = 0

Query: 1   MTSTGHTQPPSSSAPTLPWKRKLANLLSSFLFRRIFRSDFTINRRLLRLLDRKVPPSSTP 60
           M STG TQ PSSS P LP KR+LA+LLSS LFR  FRSDFT+NR  LRLLDRKVPPSST 
Sbjct: 1   MPSTGDTQSPSSS-PNLPSKRRLADLLSSVLFRHAFRSDFTVNRGFLRLLDRKVPPSST- 60

Query: 61  LHGVASFDTAAFASSPDLWARVYIPVTFAAADRLPVIVFFHGGGFVLFSTDTPQVDSFSL 120
             GVASFDT   +SS DLW RVY P+T + +DR+PVI++FHGGGFV  S D+ ++DSFS 
Sbjct: 61  TRGVASFDTTLDSSSSDLWLRVYNPLTCSESDRMPVIIYFHGGGFVFGSADSQKIDSFSR 120

Query: 121 DCAREVGAIVVSVNYRLAPEHQFPSQYDDGFHVLKAIDEGLISETLPQNADLSRCFLAGE 180
           D ARE+GAIVVSVNYRLAPEH+FPS YDDGF VLKAIDEG IS++LP+NAD+S CF+AGE
Sbjct: 121 DFAREIGAIVVSVNYRLAPEHRFPSPYDDGFRVLKAIDEGSISKSLPENADISSCFIAGE 180

Query: 181 SAGGNIAHHVAVRAAEDRQFKRLRIAGLIAIQPFFGGEERTESELRLGNGSESRLSLELT 240
           SAGGNI HHV VRAAE  +FKRL+IAG+IAIQPF GGEERT+SE+R G G    L+LE+T
Sbjct: 181 SAGGNIVHHVTVRAAE-YEFKRLKIAGMIAIQPFLGGEERTKSEIRFGKG--GGLTLEMT 240

Query: 241 DWFWKAYLPAGCDREHPAANVVVGSDISGLDFPPTLLVIGGLDPLKDRNKEYGEWLEKSG 300
           DW+WKA+LP G  R+HP AN VV SD+SGL  PP L+V GGLD L DR KEY EWLEKSG
Sbjct: 241 DWYWKAFLPIGSSRDHPGAN-VVRSDVSGLKLPPALVVNGGLDLLSDRIKEYCEWLEKSG 300

Query: 301 KEVRVVEYPNGIHGSTWNPDLPEYSTLMQDTREF 335
           KEVRVVEYPNGIHG +W  DLPEYS LMQD REF
Sbjct: 301 KEVRVVEYPNGIHGFSWFSDLPEYSMLMQDAREF 328

BLAST of Moc04g39760 vs. NCBI nr
Match: XP_004138821.1 (probable carboxylesterase 18 [Cucumis sativus] >KGN63083.1 hypothetical protein Csa_021962 [Cucumis sativus])

HSP 1 Score: 458.4 bits (1178), Expect = 5.6e-125
Identity = 223/335 (66.57%), Postives = 270/335 (80.60%), Query Frame = 0

Query: 1   MTSTGHTQPPSSSAPTLPWKRKLANLLSSFLFRRIFRSDFTINRRLLRLLDRKVPPSSTP 60
           M STG  Q P+SS PTLPWKRKLA+L +SFLFR+ FRSDFT+NRR LRL DRK+PP ++ 
Sbjct: 1   MASTGDAQSPNSSLPTLPWKRKLADLFTSFLFRQAFRSDFTVNRRFLRLFDRKLPPFTS- 60

Query: 61  LHGVASFDTAAFASSPDLWARVYIPVTFAAADRLPVIVFFHGGGFVLFSTDTPQVDSFSL 120
             GVA+ D    +S+ DLW RVY P+TF+ +D LPVI++FHGGGFV  S D P  D+F  
Sbjct: 61  -RGVAASDATIDSSTSDLWIRVYNPLTFSNSDPLPVIIYFHGGGFVYGSADAPPTDTFCR 120

Query: 121 DCAREVGAIVVSVNYRLAPEHQFPSQYDDGFHVLKAIDEGLISETLPQNADLSRCFLAGE 180
           D ARE+GAIV+SVNYRLAPE +FPSQ+DDGFHVLKA+D+G ISET+P+NADL RCF+AGE
Sbjct: 121 DFAREIGAIVISVNYRLAPEDRFPSQFDDGFHVLKAMDKGAISETVPENADLRRCFIAGE 180

Query: 181 SAGGNIAHHVAVRAAEDRQFKRLRIAGLIAIQPFFGGEERTESELRLGNGSESRLSLELT 240
           SAGGNIAHHV VRAAE  +FKR++I G+I IQPFFGGEER +SE+R G G    L+LE+T
Sbjct: 181 SAGGNIAHHVTVRAAES-EFKRVKIVGMILIQPFFGGEERRDSEIRFGRG--YGLTLEMT 240

Query: 241 DWFWKAYLPAGCDREHPAANVVVGSDISGLDFPPTLLVIGGLDPLKDRNKEYGEWLEKSG 300
           DWFWKA+LP G +R+H AAN VVGS ISG+  P  L+VIGGLD L+DRN+EY EWL+KSG
Sbjct: 241 DWFWKAWLPVGSNRDHTAAN-VVGSSISGVKVPAALVVIGGLDLLRDRNREYVEWLKKSG 300

Query: 301 KEVRVVEYPNGIHGSTWNPDLPEYSTLMQDTREFM 336
           +EVRVVEYPNG HG    PDLPEYS L+QD ++F+
Sbjct: 301 QEVRVVEYPNGTHGFIGKPDLPEYSMLIQDAKQFI 329

BLAST of Moc04g39760 vs. ExPASy Swiss-Prot
Match: Q9LT10 (Probable carboxylesterase 18 OS=Arabidopsis thaliana OX=3702 GN=CXE18 PE=1 SV=1)

HSP 1 Score: 283.9 bits (725), Expect = 2.5e-75
Identity = 153/327 (46.79%), Postives = 207/327 (63.30%), Query Frame = 0

Query: 16  TLPWKRKLANLLSSFLFRRIFRSDFTINRRLLRLLDRKVPPSSTPLHGVASFDTAAFASS 75
           TLP K ++A  + S +     R D TINRR LRL D + PP+  P++ V++ D      S
Sbjct: 12  TLPLKTRIALTVISTMTDNAQRPDGTINRRFLRLFDFRAPPNPKPVNIVSTSDFVV-DQS 71

Query: 76  PDLWARVYIPVTFAAADRLPVIVFFHGGGFVLFSTDTPQVDSFSLDCAREVGAIVVSVNY 135
            DLW R+Y P    + D++PV+VFFHGGGF   S +    D+     AR++ A V+SVNY
Sbjct: 72  RDLWFRLYTP--HVSGDKIPVVVFFHGGGFAFLSPNAYPYDNVCRRFARKLPAYVISVNY 131

Query: 136 RLAPEHQFPSQYDDGFHVLKAIDEGLISETLPQNADLSRCFLAGESAGGNIAHHVAVRAA 195
           RLAPEH++P+QYDDGF  LK I+E      LP NADLSRCF AG+SAGGNIAH+VA+R  
Sbjct: 132 RLAPEHRYPAQYDDGFDALKYIEEN-HGSILPANADLSRCFFAGDSAGGNIAHNVAIRIC 191

Query: 196 ED--RQFKRLRIAGLIAIQPFFGGEERTESELRLGNGSESRLSLELTDWFWKAYLPAGCD 255
            +    F  +++ GLI+IQPFFGGEERTE+E +L       +S + TDW WKA    G +
Sbjct: 192 REPRSSFTAVKLIGLISIQPFFGGEERTEAEKQLVGA--PLVSPDRTDWCWKA---MGLN 251

Query: 256 REHPAANV--VVGSDISGLDFPPTLLVIGGLDPLKDRNKEYGEWLEKSGKEVRVVEYPNG 315
           R+H A NV      DISGLD+P T++V+ G DPLKD  + Y EWL+  GK+  ++EYPN 
Sbjct: 252 RDHEAVNVGGPNAVDISGLDYPETMVVVAGFDPLKDWQRSYYEWLKLCGKKATLIEYPNM 311

Query: 316 IHGSTWNPDLPEYSTLMQDTREFMEHQ 339
            H     P+LPE   L+   ++F++ +
Sbjct: 312 FHAFYIFPELPEAGQLIMRIKDFVDER 329

BLAST of Moc04g39760 vs. ExPASy Swiss-Prot
Match: Q940G6 (Gibberellin receptor GID1C OS=Arabidopsis thaliana OX=3702 GN=GID1C PE=1 SV=1)

HSP 1 Score: 209.9 bits (533), Expect = 4.5e-53
Identity = 128/327 (39.14%), Postives = 183/327 (55.96%), Query Frame = 0

Query: 26  LLSSF--LFRRIFRSDFTINRRLLRLLDRKVPPSSTPLHGVASFDTAAFASSPDLWARVY 85
           L+S+F   +  + R D T NR L   LDRKVP ++ P++GV SFD        +L +RVY
Sbjct: 23  LISNFKLAYNLLRRPDGTFNRHLAEFLDRKVPANANPVNGVFSFD-VIIDRQTNLLSRVY 82

Query: 86  IPVTFAAA------------DRLPVIVFFHGGGFVLFSTDTPQVDSFSLDCAREVGAIVV 145
            P     +            + +PVIVFFHGG F   S ++   D+         GA+VV
Sbjct: 83  RPADAGTSPSITDLQNPVDGEIVPVIVFFHGGSFAHSSANSAIYDTLCRRLVGLCGAVVV 142

Query: 146 SVNYRLAPEHQFPSQYDDGFHVLKAIDEGLISETLPQNADLS-RCFLAGESAGGNIAHHV 205
           SVNYR APE+++P  YDDG+ VLK ++    S  L    D   R FLAG+S+GGNI H+V
Sbjct: 143 SVNYRRAPENRYPCAYDDGWAVLKWVNS---SSWLRSKKDSKVRIFLAGDSSGGNIVHNV 202

Query: 206 AVRAAEDRQFKRLRIAGLIAIQPFFGGEERTESELRLGNGSESRLSLELTDWFWKAYLPA 265
           AVRA E     R+ + G I + P FGG ERTESE RL    +  +++   DW+W+A+LP 
Sbjct: 203 AVRAVE----SRIDVLGNILLNPMFGGTERTESEKRL--DGKYFVTVRDRDWYWRAFLPE 262

Query: 266 GCDREHPAANVV--VGSDISGLDFPPTLLVIGGLDPLKDRNKEYGEWLEKSGKEVRVVEY 325
           G DREHPA +        + GL FP +L+V+ GLD ++D   +Y E L+K+G+EV+++  
Sbjct: 263 GEDREHPACSPFGPRSKSLEGLSFPKSLVVVAGLDLIQDWQLKYAEGLKKAGQEVKLLYL 322

Query: 326 PNGIHGSTWNPDLPEYSTLMQDTREFM 336
                G    P+   + T+M +   F+
Sbjct: 323 EQATIGFYLLPNNNHFHTVMDEIAAFV 339

BLAST of Moc04g39760 vs. ExPASy Swiss-Prot
Match: Q6L545 (Gibberellin receptor GID1 OS=Oryza sativa subsp. japonica OX=39947 GN=GID1 PE=1 SV=1)

HSP 1 Score: 203.4 bits (516), Expect = 4.2e-51
Identity = 127/335 (37.91%), Postives = 181/335 (54.03%), Query Frame = 0

Query: 26  LLSSF--LFRRIFRSDFTINRRLLRLLDRKVPPSSTPLHGVASFD--------------- 85
           L+S+F   +  + R+D T  R L   LDR+VP ++ PL GV+SFD               
Sbjct: 23  LISNFKLSYNILRRADGTFERDLGEYLDRRVPANARPLEGVSSFDHIIDQSVGLEVRIYR 82

Query: 86  TAAFASSPDLWARVYIPV-----TFAAADRLPVIVFFHGGGFVLFSTDTPQVDSFSLDCA 145
            AA   + +  A V  P+        AA+  PVI+FFHGG FV  S  +   DS      
Sbjct: 83  AAAEGDAEEGAAAVTRPILEFLTDAPAAEPFPVIIFFHGGSFVHSSASSTIYDSLCRRFV 142

Query: 146 REVGAIVVSVNYRLAPEHQFPSQYDDGFHVLKAIDEGLISETLPQNADL-SRCFLAGESA 205
           +    +VVSVNYR APEH++P  YDDG+  LK +   +    +    D  +R FL+G+S+
Sbjct: 143 KLSKGVVVSVNYRRAPEHRYPCAYDDGWTALKWV---MSQPFMRSGGDAQARVFLSGDSS 202

Query: 206 GGNIAHHVAVRAAEDRQFKRLRIAGLIAIQPFFGGEERTESELRLGNGSESRLSLELTDW 265
           GGNIAHHVAVRAA++     +++ G I +   FGG ERTESE RL    +  ++L+  DW
Sbjct: 203 GGNIAHHVAVRAADE----GVKVCGNILLNAMFGGTERTESERRL--DGKYFVTLQDRDW 262

Query: 266 FWKAYLPAGCDREHPAANVV--VGSDISGLDFPPTLLVIGGLDPLKDRNKEYGEWLEKSG 325
           +WKAYLP   DR+HPA N     G  + GL F  +L+++ GLD   DR   Y + L + G
Sbjct: 263 YWKAYLPEDADRDHPACNPFGPNGRRLGGLPFAKSLIIVSGLDLTCDRQLAYADALREDG 322

Query: 326 KEVRVVEYPNGIHGSTWNPDLPEYSTLMQDTREFM 336
             V+VV+  N   G    P+   Y  +M++  +F+
Sbjct: 323 HHVKVVQCENATVGFYLLPNTVHYHEVMEEISDFL 348

BLAST of Moc04g39760 vs. ExPASy Swiss-Prot
Match: Q9LYC1 (Gibberellin receptor GID1B OS=Arabidopsis thaliana OX=3702 GN=GID1B PE=1 SV=1)

HSP 1 Score: 199.5 bits (506), Expect = 6.1e-50
Identity = 119/337 (35.31%), Postives = 187/337 (55.49%), Query Frame = 0

Query: 26  LLSSF--LFRRIFRSDFTINRRLLRLLDRKVPPSSTPLHGVASFDTAAFASSPDLWARVY 85
           L+S+F   ++ + R D + NR L   LDRKVP +S PL GV SFD     S+ +L  R+Y
Sbjct: 23  LISNFKLAYKVLRRPDGSFNRDLAEFLDRKVPANSFPLDGVFSFDHV--DSTTNLLTRIY 82

Query: 86  IPVT---------------FAAADRLPVIVFFHGGGFVLFSTDTPQVDSFSLDCAREVGA 145
            P +                +  + +PV++FFHGG F   S ++   D+F        G 
Sbjct: 83  QPASLLHQTRHGTLELTKPLSTTEIVPVLIFFHGGSFTHSSANSAIYDTFCRRLVTICGV 142

Query: 146 IVVSVNYRLAPEHQFPSQYDDGFHVLKAIDEGLISETLPQNADLS-RCFLAGESAGGNIA 205
           +VVSV+YR +PEH++P  YDDG++ L  +   +    L    D +   +LAG+S+GGNIA
Sbjct: 143 VVVSVDYRRSPEHRYPCAYDDGWNALNWVKSRV---WLQSGKDSNVYVYLAGDSSGGNIA 202

Query: 206 HHVAVRAAEDRQFKRLRIAGLIAIQPFFGGEERTESELRLGNGSESRLSLELTDWFWKAY 265
           H+VAVRA  +     +++ G I + P FGG+ERT+SE  L    +  ++++  DW+W+AY
Sbjct: 203 HNVAVRATNE----GVKVLGNILLHPMFGGQERTQSEKTL--DGKYFVTIQDRDWYWRAY 262

Query: 266 LPAGCDREHPAANVV--VGSDISGLDFPPTLLVIGGLDPLKDRNKEYGEWLEKSGKEVRV 325
           LP G DR+HPA N     G  + G++FP +L+V+ GLD ++D    Y + L+K+G EV +
Sbjct: 263 LPEGEDRDHPACNPFGPRGQSLKGVNFPKSLVVVAGLDLVQDWQLAYVDGLKKTGLEVNL 322

Query: 326 VEYPNGIHGSTWNPDLPEYSTLMQDTREFMEHQHSKQ 343
           +       G  + P+   +  LM++  +F+      Q
Sbjct: 323 LYLKQATIGFYFLPNNDHFHCLMEELNKFVHSIEDSQ 348

BLAST of Moc04g39760 vs. ExPASy Swiss-Prot
Match: Q9MAA7 (Gibberellin receptor GID1A OS=Arabidopsis thaliana OX=3702 GN=GID1A PE=1 SV=1)

HSP 1 Score: 190.3 bits (482), Expect = 3.7e-47
Identity = 123/331 (37.16%), Postives = 175/331 (52.87%), Query Frame = 0

Query: 26  LLSSF--LFRRIFRSDFTINRRLLRLLDRKVPPSSTPLHGVASFDTAAFASSPDLWARVY 85
           L+S+F   +  + R D T NR L   LDRKV  ++ P+ GV SFD        +L +RVY
Sbjct: 23  LISNFKVAYNILRRPDGTFNRHLAEYLDRKVTANANPVDGVFSFD-VLIDRRINLLSRVY 82

Query: 86  IPVTF--------------AAADRLPVIVFFHGGGFVLFSTDTPQVDSFSLDCAREVG-- 145
            P                    D +PVI+FFHGG F   S ++   D+    C R VG  
Sbjct: 83  RPAYADQEQPPSILDLEKPVDGDIVPVILFFHGGSFAHSSANSAIYDTL---CRRLVGLC 142

Query: 146 -AIVVSVNYRLAPEHQFPSQYDDGFHVLKAIDEGLISETLPQNADLSRCFLAGESAGGNI 205
             +VVSVNYR APE+ +P  YDDG+  L  ++    S    +       FLAG+S+GGNI
Sbjct: 143 KCVVVSVNYRRAPENPYPCAYDDGWIALNWVNSR--SWLKSKKDSKVHIFLAGDSSGGNI 202

Query: 206 AHHVAVRAAEDRQFKRLRIAGLIAIQPFFGGEERTESELRLGNGSESRLSLELTDWFWKA 265
           AH+VA+RA E      + + G I + P FGG ERTESE  L    +  +++   DW+WKA
Sbjct: 203 AHNVALRAGE----SGIDVLGNILLNPMFGGNERTESEKSL--DGKYFVTVRDRDWYWKA 262

Query: 266 YLPAGCDREHPAANVVV--GSDISGLDFPPTLLVIGGLDPLKDRNKEYGEWLEKSGKEVR 325
           +LP G DREHPA N     G  + G+ FP +L+V+ GLD ++D    Y E L+K+G+EV+
Sbjct: 263 FLPEGEDREHPACNPFSPRGKSLEGVSFPKSLVVVAGLDLIRDWQLAYAEGLKKAGQEVK 322

Query: 326 VVEYPNGIHGSTWNPDLPEYSTLMQDTREFM 336
           ++       G    P+   +  +M +   F+
Sbjct: 323 LMHLEKATVGFYLLPNNNHFHNVMDEISAFV 341

BLAST of Moc04g39760 vs. ExPASy TrEMBL
Match: A0A6J1JQL4 (probable carboxylesterase 18 OS=Cucurbita maxima OX=3661 GN=LOC111487452 PE=4 SV=1)

HSP 1 Score: 475.3 bits (1222), Expect = 2.1e-130
Identity = 240/335 (71.64%), Postives = 274/335 (81.79%), Query Frame = 0

Query: 1   MTSTGHTQPPSSSAPTLPWKRKLANLLSSFLFRRIFRSDFTINRRLLRLLDRKVPPSSTP 60
           M+STG TQ PSSS P LP KRKLA+L +S LFR +FRSDFT+NR LLRLLDRKVPPS+T 
Sbjct: 1   MSSTGDTQSPSSS-PNLPSKRKLADLFTSVLFRHVFRSDFTVNRGLLRLLDRKVPPSTT- 60

Query: 61  LHGVASFDTAAFASSPDLWARVYIPVTFAAADRLPVIVFFHGGGFVLFSTDTPQVDSFSL 120
             GVASFDT   +SS DLW RVY P T + +DR+PVI++FHGGGFV  S D+ Q+DSFS 
Sbjct: 61  -RGVASFDTTLDSSSSDLWLRVYNPPTCSESDRMPVIIYFHGGGFVFGSADSQQIDSFSR 120

Query: 121 DCAREVGAIVVSVNYRLAPEHQFPSQYDDGFHVLKAIDEGLISETLPQNADLSRCFLAGE 180
           D ARE+GAIVVSVNYRLAPEH+FPS YDDGF VLKAIDEG I+++LP+NAD+SRCF+AGE
Sbjct: 121 DFAREIGAIVVSVNYRLAPEHRFPSPYDDGFRVLKAIDEGSIAKSLPENADISRCFIAGE 180

Query: 181 SAGGNIAHHVAVRAAEDRQFKRLRIAGLIAIQPFFGGEERTESELRLGNGSESRLSLELT 240
           SAGGNI HHV VRAA+  +FKRLRIAG+IAIQPF GGEERT+SE+R G G    L+LE  
Sbjct: 181 SAGGNIVHHVTVRAAK-YEFKRLRIAGMIAIQPFLGGEERTQSEIRFGRG--GGLTLETA 240

Query: 241 DWFWKAYLPAGCDREHPAANVV-VGSDISGLDFPPTLLVIGGLDPLKDRNKEYGEWLEKS 300
           DW+WKA+LP G  R+HPAANVV  GSD+SGL  PP L+V GGLD L DR KEY EWLEKS
Sbjct: 241 DWYWKAFLPIGSSRDHPAANVVRSGSDVSGLKLPPALVVNGGLDLLSDRIKEYCEWLEKS 300

Query: 301 GKEVRVVEYPNGIHGSTWNPDLPEYSTLMQDTREF 335
           GKEVRVVEYPNGIHG +W  DLPEYS LMQD REF
Sbjct: 301 GKEVRVVEYPNGIHGFSWFSDLPEYSMLMQDAREF 329

BLAST of Moc04g39760 vs. ExPASy TrEMBL
Match: A0A6J1HPS2 (probable carboxylesterase 18 OS=Cucurbita moschata OX=3662 GN=LOC111464932 PE=4 SV=1)

HSP 1 Score: 471.5 bits (1212), Expect = 3.1e-129
Identity = 239/334 (71.56%), Postives = 272/334 (81.44%), Query Frame = 0

Query: 1   MTSTGHTQPPSSSAPTLPWKRKLANLLSSFLFRRIFRSDFTINRRLLRLLDRKVPPSSTP 60
           M STG TQ PSSS P LP KR+LA+LLSS LFR  FRSDFT+NR  LRLLDRKVPPSST 
Sbjct: 1   MPSTGDTQSPSSS-PNLPSKRRLADLLSSVLFRHAFRSDFTVNRGFLRLLDRKVPPSST- 60

Query: 61  LHGVASFDTAAFASSPDLWARVYIPVTFAAADRLPVIVFFHGGGFVLFSTDTPQVDSFSL 120
             GVASFDT   +SS DLW RVY P+T + +DR+PVI++FHGGGFV  S D+ ++DSFS 
Sbjct: 61  TRGVASFDTTLDSSSSDLWLRVYNPLTCSESDRMPVIIYFHGGGFVFGSADSQKIDSFSR 120

Query: 121 DCAREVGAIVVSVNYRLAPEHQFPSQYDDGFHVLKAIDEGLISETLPQNADLSRCFLAGE 180
           D ARE+GAIVVSVNYRLAPEH+FPS YDDGF VLKAIDEG IS++LP+NAD+S CF+AGE
Sbjct: 121 DFAREIGAIVVSVNYRLAPEHRFPSPYDDGFRVLKAIDEGSISKSLPENADISSCFIAGE 180

Query: 181 SAGGNIAHHVAVRAAEDRQFKRLRIAGLIAIQPFFGGEERTESELRLGNGSESRLSLELT 240
           SAGGNI HHV VRAAE  +FKRL+IAG+IAIQPF GGEERT+SE+R G G    L+LE+T
Sbjct: 181 SAGGNIVHHVTVRAAE-YEFKRLKIAGMIAIQPFLGGEERTKSEIRFGKG--GGLTLEMT 240

Query: 241 DWFWKAYLPAGCDREHPAANVVVGSDISGLDFPPTLLVIGGLDPLKDRNKEYGEWLEKSG 300
           DW+WKA+LP G  R+HP AN VV SD+SGL  PP L+V GGLD L DR KEY EWLEKSG
Sbjct: 241 DWYWKAFLPIGSSRDHPGAN-VVRSDVSGLKLPPALVVNGGLDLLSDRIKEYCEWLEKSG 300

Query: 301 KEVRVVEYPNGIHGSTWNPDLPEYSTLMQDTREF 335
           KEVRVVEYPNGIHG +W  DLPEYS LMQD REF
Sbjct: 301 KEVRVVEYPNGIHGFSWFSDLPEYSMLMQDAREF 328

BLAST of Moc04g39760 vs. ExPASy TrEMBL
Match: A0A0A0LMQ6 (Abhydrolase_3 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G401430 PE=4 SV=1)

HSP 1 Score: 458.4 bits (1178), Expect = 2.7e-125
Identity = 223/335 (66.57%), Postives = 270/335 (80.60%), Query Frame = 0

Query: 1   MTSTGHTQPPSSSAPTLPWKRKLANLLSSFLFRRIFRSDFTINRRLLRLLDRKVPPSSTP 60
           M STG  Q P+SS PTLPWKRKLA+L +SFLFR+ FRSDFT+NRR LRL DRK+PP ++ 
Sbjct: 1   MASTGDAQSPNSSLPTLPWKRKLADLFTSFLFRQAFRSDFTVNRRFLRLFDRKLPPFTS- 60

Query: 61  LHGVASFDTAAFASSPDLWARVYIPVTFAAADRLPVIVFFHGGGFVLFSTDTPQVDSFSL 120
             GVA+ D    +S+ DLW RVY P+TF+ +D LPVI++FHGGGFV  S D P  D+F  
Sbjct: 61  -RGVAASDATIDSSTSDLWIRVYNPLTFSNSDPLPVIIYFHGGGFVYGSADAPPTDTFCR 120

Query: 121 DCAREVGAIVVSVNYRLAPEHQFPSQYDDGFHVLKAIDEGLISETLPQNADLSRCFLAGE 180
           D ARE+GAIV+SVNYRLAPE +FPSQ+DDGFHVLKA+D+G ISET+P+NADL RCF+AGE
Sbjct: 121 DFAREIGAIVISVNYRLAPEDRFPSQFDDGFHVLKAMDKGAISETVPENADLRRCFIAGE 180

Query: 181 SAGGNIAHHVAVRAAEDRQFKRLRIAGLIAIQPFFGGEERTESELRLGNGSESRLSLELT 240
           SAGGNIAHHV VRAAE  +FKR++I G+I IQPFFGGEER +SE+R G G    L+LE+T
Sbjct: 181 SAGGNIAHHVTVRAAES-EFKRVKIVGMILIQPFFGGEERRDSEIRFGRG--YGLTLEMT 240

Query: 241 DWFWKAYLPAGCDREHPAANVVVGSDISGLDFPPTLLVIGGLDPLKDRNKEYGEWLEKSG 300
           DWFWKA+LP G +R+H AAN VVGS ISG+  P  L+VIGGLD L+DRN+EY EWL+KSG
Sbjct: 241 DWFWKAWLPVGSNRDHTAAN-VVGSSISGVKVPAALVVIGGLDLLRDRNREYVEWLKKSG 300

Query: 301 KEVRVVEYPNGIHGSTWNPDLPEYSTLMQDTREFM 336
           +EVRVVEYPNG HG    PDLPEYS L+QD ++F+
Sbjct: 301 QEVRVVEYPNGTHGFIGKPDLPEYSMLIQDAKQFI 329

BLAST of Moc04g39760 vs. ExPASy TrEMBL
Match: A0A5A7T4I6 (Putative carboxylesterase 18 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold453G00840 PE=4 SV=1)

HSP 1 Score: 454.9 bits (1169), Expect = 3.0e-124
Identity = 226/331 (68.28%), Postives = 265/331 (80.06%), Query Frame = 0

Query: 1   MTSTGHTQPPSSSAPTLPWKRKLANLLSSFLFRRIFRSDFTINRRLLRLLDRKVPPSSTP 60
           M S G  Q P+SS PTLPW RKLA+L +SFLFRR FRSDFT+NRRLL LLDRKVPP +T 
Sbjct: 1   MASAGDAQSPNSSPPTLPWNRKLADLFTSFLFRRAFRSDFTVNRRLLGLLDRKVPPFTT- 60

Query: 61  LHGVASFDTAAFASSPD-LWARVYIPVTFAAADRLPVIVFFHGGGFVLFSTDTPQVDSFS 120
             GVA+ D    +SS D LW RVY P+TF  +D LPVIVFFHGGGFV  S D+P +D+F 
Sbjct: 61  -RGVAASDMNIDSSSSDHLWIRVYNPLTFFNSDPLPVIVFFHGGGFVYGSADSPPIDTFC 120

Query: 121 LDCAREVGAIVVSVNYRLAPEHQFPSQYDDGFHVLKAIDEGLISETLPQNADLSRCFLAG 180
            D ARE+GAIV+SVNYRLAPEH+FPSQ+DDGFHVLKA++EG+ISETLP+NADL RCF+AG
Sbjct: 121 RDFAREIGAIVISVNYRLAPEHRFPSQFDDGFHVLKAMEEGVISETLPENADLRRCFIAG 180

Query: 181 ESAGGNIAHHVAVRAAEDRQFKRLRIAGLIAIQPFFGGEERTESELRLGNGSESRLSLEL 240
           ESAGGNIAHHV VRAAE  + KRL+I G+I IQPF GGEER +SE+R G G    +++E+
Sbjct: 181 ESAGGNIAHHVTVRAAEG-ELKRLKIVGMILIQPFLGGEERRDSEIRFGRG--YGMTVEM 240

Query: 241 TDWFWKAYLPAGCDREHPAANVVVGSDISGLDFPPTLLVIGGLDPLKDRNKEYGEWLEKS 300
            DWFWKA+LP G +R+HPAAN VV S ISG+  PP ++VIGGLD L+DR KEY EWL+K 
Sbjct: 241 ADWFWKAWLPVGSNRDHPAAN-VVRSSISGVKLPPAVVVIGGLDLLRDRQKEYVEWLKKW 300

Query: 301 GKEVRVVEYPNGIHGSTWNPDLPEYSTLMQD 331
           G+EVRVVEYPNG HG  W PDLPEYS LMQD
Sbjct: 301 GEEVRVVEYPNGTHGFIWKPDLPEYSMLMQD 325

BLAST of Moc04g39760 vs. ExPASy TrEMBL
Match: A0A1S3B2W2 (probable carboxylesterase 18 OS=Cucumis melo OX=3656 GN=LOC103485400 PE=4 SV=1)

HSP 1 Score: 454.9 bits (1169), Expect = 3.0e-124
Identity = 226/331 (68.28%), Postives = 265/331 (80.06%), Query Frame = 0

Query: 1   MTSTGHTQPPSSSAPTLPWKRKLANLLSSFLFRRIFRSDFTINRRLLRLLDRKVPPSSTP 60
           M S G  Q P+SS PTLPW RKLA+L +SFLFRR FRSDFT+NRRLL LLDRKVPP +T 
Sbjct: 1   MASAGDAQSPNSSPPTLPWNRKLADLFTSFLFRRAFRSDFTVNRRLLGLLDRKVPPFTT- 60

Query: 61  LHGVASFDTAAFASSPD-LWARVYIPVTFAAADRLPVIVFFHGGGFVLFSTDTPQVDSFS 120
             GVA+ D    +SS D LW RVY P+TF  +D LPVIVFFHGGGFV  S D+P +D+F 
Sbjct: 61  -RGVAASDMNIDSSSSDHLWIRVYNPLTFFNSDPLPVIVFFHGGGFVYGSADSPPIDTFC 120

Query: 121 LDCAREVGAIVVSVNYRLAPEHQFPSQYDDGFHVLKAIDEGLISETLPQNADLSRCFLAG 180
            D ARE+GAIV+SVNYRLAPEH+FPSQ+DDGFHVLKA++EG+ISETLP+NADL RCF+AG
Sbjct: 121 RDFAREIGAIVISVNYRLAPEHRFPSQFDDGFHVLKAMEEGVISETLPENADLRRCFIAG 180

Query: 181 ESAGGNIAHHVAVRAAEDRQFKRLRIAGLIAIQPFFGGEERTESELRLGNGSESRLSLEL 240
           ESAGGNIAHHV VRAAE  + KRL+I G+I IQPF GGEER +SE+R G G    +++E+
Sbjct: 181 ESAGGNIAHHVTVRAAEG-ELKRLKIVGMILIQPFLGGEERRDSEIRFGRG--YGMTVEM 240

Query: 241 TDWFWKAYLPAGCDREHPAANVVVGSDISGLDFPPTLLVIGGLDPLKDRNKEYGEWLEKS 300
            DWFWKA+LP G +R+HPAAN VV S ISG+  PP ++VIGGLD L+DR KEY EWL+K 
Sbjct: 241 ADWFWKAWLPVGSNRDHPAAN-VVRSSISGVKLPPAVVVIGGLDLLRDRQKEYVEWLKKW 300

Query: 301 GKEVRVVEYPNGIHGSTWNPDLPEYSTLMQD 331
           G+EVRVVEYPNG HG  W PDLPEYS LMQD
Sbjct: 301 GEEVRVVEYPNGTHGFIWKPDLPEYSMLMQD 325

BLAST of Moc04g39760 vs. TAIR 10
Match: AT5G23530.1 (carboxyesterase 18 )

HSP 1 Score: 283.9 bits (725), Expect = 1.7e-76
Identity = 153/327 (46.79%), Postives = 207/327 (63.30%), Query Frame = 0

Query: 16  TLPWKRKLANLLSSFLFRRIFRSDFTINRRLLRLLDRKVPPSSTPLHGVASFDTAAFASS 75
           TLP K ++A  + S +     R D TINRR LRL D + PP+  P++ V++ D      S
Sbjct: 12  TLPLKTRIALTVISTMTDNAQRPDGTINRRFLRLFDFRAPPNPKPVNIVSTSDFVV-DQS 71

Query: 76  PDLWARVYIPVTFAAADRLPVIVFFHGGGFVLFSTDTPQVDSFSLDCAREVGAIVVSVNY 135
            DLW R+Y P    + D++PV+VFFHGGGF   S +    D+     AR++ A V+SVNY
Sbjct: 72  RDLWFRLYTP--HVSGDKIPVVVFFHGGGFAFLSPNAYPYDNVCRRFARKLPAYVISVNY 131

Query: 136 RLAPEHQFPSQYDDGFHVLKAIDEGLISETLPQNADLSRCFLAGESAGGNIAHHVAVRAA 195
           RLAPEH++P+QYDDGF  LK I+E      LP NADLSRCF AG+SAGGNIAH+VA+R  
Sbjct: 132 RLAPEHRYPAQYDDGFDALKYIEEN-HGSILPANADLSRCFFAGDSAGGNIAHNVAIRIC 191

Query: 196 ED--RQFKRLRIAGLIAIQPFFGGEERTESELRLGNGSESRLSLELTDWFWKAYLPAGCD 255
            +    F  +++ GLI+IQPFFGGEERTE+E +L       +S + TDW WKA    G +
Sbjct: 192 REPRSSFTAVKLIGLISIQPFFGGEERTEAEKQLVGA--PLVSPDRTDWCWKA---MGLN 251

Query: 256 REHPAANV--VVGSDISGLDFPPTLLVIGGLDPLKDRNKEYGEWLEKSGKEVRVVEYPNG 315
           R+H A NV      DISGLD+P T++V+ G DPLKD  + Y EWL+  GK+  ++EYPN 
Sbjct: 252 RDHEAVNVGGPNAVDISGLDYPETMVVVAGFDPLKDWQRSYYEWLKLCGKKATLIEYPNM 311

Query: 316 IHGSTWNPDLPEYSTLMQDTREFMEHQ 339
            H     P+LPE   L+   ++F++ +
Sbjct: 312 FHAFYIFPELPEAGQLIMRIKDFVDER 329

BLAST of Moc04g39760 vs. TAIR 10
Match: AT5G27320.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 209.9 bits (533), Expect = 3.2e-54
Identity = 128/327 (39.14%), Postives = 183/327 (55.96%), Query Frame = 0

Query: 26  LLSSF--LFRRIFRSDFTINRRLLRLLDRKVPPSSTPLHGVASFDTAAFASSPDLWARVY 85
           L+S+F   +  + R D T NR L   LDRKVP ++ P++GV SFD        +L +RVY
Sbjct: 23  LISNFKLAYNLLRRPDGTFNRHLAEFLDRKVPANANPVNGVFSFD-VIIDRQTNLLSRVY 82

Query: 86  IPVTFAAA------------DRLPVIVFFHGGGFVLFSTDTPQVDSFSLDCAREVGAIVV 145
            P     +            + +PVIVFFHGG F   S ++   D+         GA+VV
Sbjct: 83  RPADAGTSPSITDLQNPVDGEIVPVIVFFHGGSFAHSSANSAIYDTLCRRLVGLCGAVVV 142

Query: 146 SVNYRLAPEHQFPSQYDDGFHVLKAIDEGLISETLPQNADLS-RCFLAGESAGGNIAHHV 205
           SVNYR APE+++P  YDDG+ VLK ++    S  L    D   R FLAG+S+GGNI H+V
Sbjct: 143 SVNYRRAPENRYPCAYDDGWAVLKWVNS---SSWLRSKKDSKVRIFLAGDSSGGNIVHNV 202

Query: 206 AVRAAEDRQFKRLRIAGLIAIQPFFGGEERTESELRLGNGSESRLSLELTDWFWKAYLPA 265
           AVRA E     R+ + G I + P FGG ERTESE RL    +  +++   DW+W+A+LP 
Sbjct: 203 AVRAVE----SRIDVLGNILLNPMFGGTERTESEKRL--DGKYFVTVRDRDWYWRAFLPE 262

Query: 266 GCDREHPAANVV--VGSDISGLDFPPTLLVIGGLDPLKDRNKEYGEWLEKSGKEVRVVEY 325
           G DREHPA +        + GL FP +L+V+ GLD ++D   +Y E L+K+G+EV+++  
Sbjct: 263 GEDREHPACSPFGPRSKSLEGLSFPKSLVVVAGLDLIQDWQLKYAEGLKKAGQEVKLLYL 322

Query: 326 PNGIHGSTWNPDLPEYSTLMQDTREFM 336
                G    P+   + T+M +   F+
Sbjct: 323 EQATIGFYLLPNNNHFHTVMDEIAAFV 339

BLAST of Moc04g39760 vs. TAIR 10
Match: AT3G63010.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 199.5 bits (506), Expect = 4.3e-51
Identity = 119/337 (35.31%), Postives = 187/337 (55.49%), Query Frame = 0

Query: 26  LLSSF--LFRRIFRSDFTINRRLLRLLDRKVPPSSTPLHGVASFDTAAFASSPDLWARVY 85
           L+S+F   ++ + R D + NR L   LDRKVP +S PL GV SFD     S+ +L  R+Y
Sbjct: 23  LISNFKLAYKVLRRPDGSFNRDLAEFLDRKVPANSFPLDGVFSFDHV--DSTTNLLTRIY 82

Query: 86  IPVT---------------FAAADRLPVIVFFHGGGFVLFSTDTPQVDSFSLDCAREVGA 145
            P +                +  + +PV++FFHGG F   S ++   D+F        G 
Sbjct: 83  QPASLLHQTRHGTLELTKPLSTTEIVPVLIFFHGGSFTHSSANSAIYDTFCRRLVTICGV 142

Query: 146 IVVSVNYRLAPEHQFPSQYDDGFHVLKAIDEGLISETLPQNADLS-RCFLAGESAGGNIA 205
           +VVSV+YR +PEH++P  YDDG++ L  +   +    L    D +   +LAG+S+GGNIA
Sbjct: 143 VVVSVDYRRSPEHRYPCAYDDGWNALNWVKSRV---WLQSGKDSNVYVYLAGDSSGGNIA 202

Query: 206 HHVAVRAAEDRQFKRLRIAGLIAIQPFFGGEERTESELRLGNGSESRLSLELTDWFWKAY 265
           H+VAVRA  +     +++ G I + P FGG+ERT+SE  L    +  ++++  DW+W+AY
Sbjct: 203 HNVAVRATNE----GVKVLGNILLHPMFGGQERTQSEKTL--DGKYFVTIQDRDWYWRAY 262

Query: 266 LPAGCDREHPAANVV--VGSDISGLDFPPTLLVIGGLDPLKDRNKEYGEWLEKSGKEVRV 325
           LP G DR+HPA N     G  + G++FP +L+V+ GLD ++D    Y + L+K+G EV +
Sbjct: 263 LPEGEDRDHPACNPFGPRGQSLKGVNFPKSLVVVAGLDLVQDWQLAYVDGLKKTGLEVNL 322

Query: 326 VEYPNGIHGSTWNPDLPEYSTLMQDTREFMEHQHSKQ 343
           +       G  + P+   +  LM++  +F+      Q
Sbjct: 323 LYLKQATIGFYFLPNNDHFHCLMEELNKFVHSIEDSQ 348

BLAST of Moc04g39760 vs. TAIR 10
Match: AT3G05120.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 190.3 bits (482), Expect = 2.6e-48
Identity = 123/331 (37.16%), Postives = 175/331 (52.87%), Query Frame = 0

Query: 26  LLSSF--LFRRIFRSDFTINRRLLRLLDRKVPPSSTPLHGVASFDTAAFASSPDLWARVY 85
           L+S+F   +  + R D T NR L   LDRKV  ++ P+ GV SFD        +L +RVY
Sbjct: 23  LISNFKVAYNILRRPDGTFNRHLAEYLDRKVTANANPVDGVFSFD-VLIDRRINLLSRVY 82

Query: 86  IPVTF--------------AAADRLPVIVFFHGGGFVLFSTDTPQVDSFSLDCAREVG-- 145
            P                    D +PVI+FFHGG F   S ++   D+    C R VG  
Sbjct: 83  RPAYADQEQPPSILDLEKPVDGDIVPVILFFHGGSFAHSSANSAIYDTL---CRRLVGLC 142

Query: 146 -AIVVSVNYRLAPEHQFPSQYDDGFHVLKAIDEGLISETLPQNADLSRCFLAGESAGGNI 205
             +VVSVNYR APE+ +P  YDDG+  L  ++    S    +       FLAG+S+GGNI
Sbjct: 143 KCVVVSVNYRRAPENPYPCAYDDGWIALNWVNSR--SWLKSKKDSKVHIFLAGDSSGGNI 202

Query: 206 AHHVAVRAAEDRQFKRLRIAGLIAIQPFFGGEERTESELRLGNGSESRLSLELTDWFWKA 265
           AH+VA+RA E      + + G I + P FGG ERTESE  L    +  +++   DW+WKA
Sbjct: 203 AHNVALRAGE----SGIDVLGNILLNPMFGGNERTESEKSL--DGKYFVTVRDRDWYWKA 262

Query: 266 YLPAGCDREHPAANVVV--GSDISGLDFPPTLLVIGGLDPLKDRNKEYGEWLEKSGKEVR 325
           +LP G DREHPA N     G  + G+ FP +L+V+ GLD ++D    Y E L+K+G+EV+
Sbjct: 263 FLPEGEDREHPACNPFSPRGKSLEGVSFPKSLVVVAGLDLIRDWQLAYAEGLKKAGQEVK 322

Query: 326 VVEYPNGIHGSTWNPDLPEYSTLMQDTREFM 336
           ++       G    P+   +  +M +   F+
Sbjct: 323 LMHLEKATVGFYLLPNNNHFHNVMDEISAFV 341

BLAST of Moc04g39760 vs. TAIR 10
Match: AT5G06570.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 179.1 bits (453), Expect = 6.1e-45
Identity = 101/259 (39.00%), Postives = 147/259 (56.76%), Query Frame = 0

Query: 67  FDTAAFASSPDLWARVYIPVTFAAADRLPVIVFFHGGGFVLFSTDTPQVDSFSLDCAREV 126
           F  + +    +L  R+Y P++ +    LPV+VFFHGGGF   S   P   +F L  A  +
Sbjct: 49  FKDSIYHKPNNLHLRLYKPISASNRTALPVVVFFHGGGFCFGSRSWPHFHNFCLTLASSL 108

Query: 127 GAIVVSVNYRLAPEHQFPSQYDDGFHVLKAIDEGLISETLPQ------NADLSRCFLAGE 186
            A+VVS +YRLAPEH+ P+ ++D   VL  + +  +S+ +        + D  R F+ G+
Sbjct: 109 NALVVSPDYRLAPEHRLPAAFEDAEAVLTWLWDQAVSDGVNHWFEDGTDVDFDRVFVVGD 168

Query: 187 SAGGNIAHHVAVR-AAEDRQFKRLRIAGLIAIQPFFGGEERTESELRLGNG-SESRLSLE 246
           S+GGNIAH +AVR  +   +   +R+ G + + PFFGGEERT SE    NG SE+ LSL+
Sbjct: 169 SSGGNIAHQLAVRFGSGSIELTPVRVRGYVLMGPFFGGEERTNSE----NGPSEALLSLD 228

Query: 247 LTDWFWKAYLPAGCDREHPAANVV--VGSDISGLDFPPTLLVIGGLDPLKDRNKEYGEWL 306
           L D FW+  LP G  R+H  AN        +  +   P L+++GG + L+DR KEY   L
Sbjct: 229 LLDKFWRLSLPNGATRDHHMANPFGPTSPTLESISLEPMLVIVGGSELLRDRAKEYAYKL 288

Query: 307 EK-SGKEVRVVEYPNGIHG 315
           +K  GK V  +E+ N  HG
Sbjct: 289 KKMGGKRVDYIEFENKEHG 303

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_023549493.11.8e-13171.30probable carboxylesterase 18 [Cucurbita pepo subsp. pepo][more]
XP_022990625.14.4e-13071.64probable carboxylesterase 18 [Cucurbita maxima][more]
KAG6602724.19.8e-13072.46putative carboxylesterase 18, partial [Cucurbita argyrosperma subsp. sororia] >K... [more]
XP_022964989.16.4e-12971.56probable carboxylesterase 18 [Cucurbita moschata][more]
XP_004138821.15.6e-12566.57probable carboxylesterase 18 [Cucumis sativus] >KGN63083.1 hypothetical protein ... [more]
Match NameE-valueIdentityDescription
Q9LT102.5e-7546.79Probable carboxylesterase 18 OS=Arabidopsis thaliana OX=3702 GN=CXE18 PE=1 SV=1[more]
Q940G64.5e-5339.14Gibberellin receptor GID1C OS=Arabidopsis thaliana OX=3702 GN=GID1C PE=1 SV=1[more]
Q6L5454.2e-5137.91Gibberellin receptor GID1 OS=Oryza sativa subsp. japonica OX=39947 GN=GID1 PE=1 ... [more]
Q9LYC16.1e-5035.31Gibberellin receptor GID1B OS=Arabidopsis thaliana OX=3702 GN=GID1B PE=1 SV=1[more]
Q9MAA73.7e-4737.16Gibberellin receptor GID1A OS=Arabidopsis thaliana OX=3702 GN=GID1A PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1JQL42.1e-13071.64probable carboxylesterase 18 OS=Cucurbita maxima OX=3661 GN=LOC111487452 PE=4 SV... [more]
A0A6J1HPS23.1e-12971.56probable carboxylesterase 18 OS=Cucurbita moschata OX=3662 GN=LOC111464932 PE=4 ... [more]
A0A0A0LMQ62.7e-12566.57Abhydrolase_3 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G4014... [more]
A0A5A7T4I63.0e-12468.28Putative carboxylesterase 18 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_sca... [more]
A0A1S3B2W23.0e-12468.28probable carboxylesterase 18 OS=Cucumis melo OX=3656 GN=LOC103485400 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
AT5G23530.11.7e-7646.79carboxyesterase 18 [more]
AT5G27320.13.2e-5439.14alpha/beta-Hydrolases superfamily protein [more]
AT3G63010.14.3e-5135.31alpha/beta-Hydrolases superfamily protein [more]
AT3G05120.12.6e-4837.16alpha/beta-Hydrolases superfamily protein [more]
AT5G06570.16.1e-4539.00alpha/beta-Hydrolases superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (OHB3-1) v2
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR013094Alpha/beta hydrolase fold-3PFAMPF07859Abhydrolase_3coord: 97..314
e-value: 1.4E-58
score: 198.3
IPR029058Alpha/Beta hydrolase foldGENE3D3.40.50.1820alpha/beta hydrolasecoord: 9..344
e-value: 5.3E-87
score: 294.3
IPR029058Alpha/Beta hydrolase foldSUPERFAMILY53474alpha/beta-Hydrolasescoord: 52..336
NoneNo IPR availablePANTHERPTHR23024:SF434ACETYL ESTERASEcoord: 12..338
NoneNo IPR availablePANTHERPTHR23024ARYLACETAMIDE DEACETYLASEcoord: 12..338

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Moc04g39760.1Moc04g39760.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0016787 hydrolase activity