Moc04g39510 (gene) Bitter gourd (OHB3-1) v2

Overview
NameMoc04g39510
Typegene
OrganismMomordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
Descriptionalkylated DNA repair protein alkB homolog 8 isoform X1
Locationchr4: 29339925 .. 29342287 (+)
RNA-Seq ExpressionMoc04g39510
SyntenyMoc04g39510
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGCCTCAAGCTTGTAGTCGTGCTTCAAGAGTGAGCAAATTCTTTTACCAAAATAGCAAGATCTTTGATTTATCCTCTGTTGTAATTGGCAGTGGGCATCATTTTAGACTTTTCAGTGCTATGAAAGAAATTAAAGTCAAAGCTGGTTCTAAGTCAGATCCTCATCTGTCAAATGATGAAACAAATGCACAACAATCATCTTGTGCAAATCAGGGATGCTCATCAAACGTGCAGTGCACCCCTGAAATTGAAAAGAAGTATGTGCATCGTGTTTATGATGCCATTGCACCCCATTTTAGTTCCACTCGATTTGCGAAGTGGCCAAAAGTTGCAGCATTCTTATCCTCATTGCCTCTAGGCTCCCTCATCCTGGATGCTGGCTGTGGTAATGGAAAGTACTTGGGTTTTACTCCCAACTGCTTTTTTATAGGATGTGATATTAGTGCTCAACTTATCAAAATTTGTAAAGAAAGGGGGCATGATGTTCTGGTGGCTGATGCAGTAAATCTTCCTTACAGAACTGGTTTTGGCGATGCAGCAATCTCTATAGCTGTATTACATCATCTAAGTACTGAGAACAGGAGGAAGAAAGCAATTGAAGAATTAATACGTGTTGTTAAAAAAGGTGGCCTAGTTTTAATTACAGTGTGGGCTGTTGAACAGGAGGATAAATCTTTATTGACGAAATGGATGCCACTTACAGAGAAGTATGTTGAAGAGTGGGTTGGACCAGGTAGTCCCCGTGTTCGTAGTCCTTCATCATTGGCACTTGAAAGCATTCCTGAAATGAATGAAAATGGTTCAGGTATTCATCTAAGGGATTCCAAGGAGAATTTCACTGGAAGTATGCCAGAAAATAAATCTCTACCATCTCAAAGTGGAGACGAGTTGCCGAGTTGTATTGATGAAAACTTATCAAAGACTGCCCAGCAAGAATATTTTGTACCATGGCACTTACCTTATCATCGTGCAGAAGTCAGCGGCACGTCTGCCAGTGCCCTTGCCAGTGGTTTAGCAAAGAAAGATGATAAAAAGGCTGCTGTTGTGTATAACAGATACTATCATGTTTTCAGTGAAGGCGAGCTTGAAAGGTATTCCTCTCTCTCATATCTATTGTATGGTTTTCATTTTCTGGTCTCCTTTTGCTTATTGGGTACGAGTGAATCACTTGAATAGATCCGGTGCCCTCTTGCATGAGTTCTATAATCTATAATGTAACAAATATCTCTTTACAGATGTGACTGTGCTGTTCTAGATATGTTCCATCTCTCATTTCCTCCCTCTAATACTCAAACATGAGCCATATATTTATGTTTAAACATAGCTGGATGAAAGTTGGACAAAAAGGGAATAATGTAGACATTTTAATATATGTGCAAAAAAAATAAATCATCTTCATAATGCACAAAGCAACAGGGTTGAAAGTAGTCTCGGTGGTAGAAAAGGTTGTTTTTGCACATGGGAGATGGCGCCGGTACTGTTGATTACCCCTTTTAGTCCAAGTTGGAAATATATGGAAAAAGAACATTATACTTTTCCTAGCTTTGACTTATTGTTTCCCTACGAGCAAAATCAATCAGAAATTGCTACCGTGAAAAAGAGATTTTTACAAAAGAGGAAAGGGGAGATAATAAGGCATCCATACCCAAGACAAAAAGGATCTTCACCAATTGGTAGCAATCTAAAAGGGGGCTACAGTATTAAGACCAAAATAAGAAGAAAATAAGGAAAAGTTGAACCTAACCTTTTGGCTGAAAGTTGGCCCTACCAAATTTCCTCTGCTATATTAGATGTTTACTTGGGGGCACCTATTGGAAGTTTGCCTTGTATAATTTGCACGTGGAGTAAATGTTAATATATGTATGCAGTAGCTTCCATTAATGTGTGCTCAGGTGATAATCAATTTTCTATCATTCCATAGTTTGACTTGAAATACCATCAACTTGAAATATAGGGAACTCAGTATACCATACTTCAAGACTTTAACTTATTTCACCTTCCTTTACCCCCCTCGAGATACGTTTCTTACGTAATATTTTGTTCTTTTACTGTTTGGGTGCAGGCTAGTTTCTGGTATGGACAACGCAGTAGTGATCGATCGATTTTATGACAAGTCAAACTGGTGTATAGTTCTTGAGAAAACAGTGTGACCTATGGAATGAGCCCACTGGCCTATATGAAGATGATTCCAACGTGGACGTGCCTGCCAACTCAAATCGTCATTATTTCAAAATGAACTCGCGGTGCTGAAGGTGGTATCCCTTGATGTTTATTGCTCTTGCTATCTCGGATCTGTTCTTGGCCAATCCTGGTTTGGAGATGGAGATCTGCAGGTTCCCTTGAAGAAAATGCATGCACTGTAA

mRNA sequence

ATGCCTCAAGCTTGTAGTCGTGCTTCAAGAGTGAGCAAATTCTTTTACCAAAATAGCAAGATCTTTGATTTATCCTCTGTTGTAATTGGCAGTGGGCATCATTTTAGACTTTTCAGTGCTATGAAAGAAATTAAAGTCAAAGCTGGTTCTAAGTCAGATCCTCATCTGTCAAATGATGAAACAAATGCACAACAATCATCTTGTGCAAATCAGGGATGCTCATCAAACGTGCAGTGCACCCCTGAAATTGAAAAGAAGTATGTGCATCGTGTTTATGATGCCATTGCACCCCATTTTAGTTCCACTCGATTTGCGAAGTGGCCAAAAGTTGCAGCATTCTTATCCTCATTGCCTCTAGGCTCCCTCATCCTGGATGCTGGCTGTGGTAATGGAAAGTACTTGGGTTTTACTCCCAACTGCTTTTTTATAGGATGTGATATTAGTGCTCAACTTATCAAAATTTGTAAAGAAAGGGGGCATGATGTTCTGGTGGCTGATGCAGTAAATCTTCCTTACAGAACTGGTTTTGGCGATGCAGCAATCTCTATAGCTGTATTACATCATCTAAGTACTGAGAACAGGAGGAAGAAAGCAATTGAAGAATTAATACGTGTTGTTAAAAAAGGTGGCCTAGTTTTAATTACAGTGTGGGCTGTTGAACAGGAGGATAAATCTTTATTGACGAAATGGATGCCACTTACAGAGAAGTATGTTGAAGAGTGGGTTGGACCAGGTATTCATCTAAGGGATTCCAAGGAGAATTTCACTGGAAGTATGCCAGAAAATAAATCTCTACCATCTCAAAGTGGAGACGAGTTGCCGAGTTGTATTGATGAAAACTTATCAAAGACTGCCCAGCAAGAATATTTTGTACCATGGCACTTACCTTATCATCGTGCAGAAGTCAGCGGCACGTCTGCCAGTGCCCTTGCCAGTGGTTTAGCAAAGAAAGATGATAAAAAGGCTGCTGTTGTGTATAACAGATACTATCATGTTTTCAGTGAAGGCGAGCTTGAAAGGCTAGTTTCTGGTATGGACAACGCAGTAGTGATCGATCGATTTTATGACAAGTCAAACTGGTGTATAGTTCTTGAGAAAACAGTGGTATCCCTTGATGTTTATTGCTCTTGCTATCTCGGATCTGTTCTTGGCCAATCCTGGTTTGGAGATGGAGATCTGCAGGTTCCCTTGAAGAAAATGCATGCACTGTAA

Coding sequence (CDS)

ATGCCTCAAGCTTGTAGTCGTGCTTCAAGAGTGAGCAAATTCTTTTACCAAAATAGCAAGATCTTTGATTTATCCTCTGTTGTAATTGGCAGTGGGCATCATTTTAGACTTTTCAGTGCTATGAAAGAAATTAAAGTCAAAGCTGGTTCTAAGTCAGATCCTCATCTGTCAAATGATGAAACAAATGCACAACAATCATCTTGTGCAAATCAGGGATGCTCATCAAACGTGCAGTGCACCCCTGAAATTGAAAAGAAGTATGTGCATCGTGTTTATGATGCCATTGCACCCCATTTTAGTTCCACTCGATTTGCGAAGTGGCCAAAAGTTGCAGCATTCTTATCCTCATTGCCTCTAGGCTCCCTCATCCTGGATGCTGGCTGTGGTAATGGAAAGTACTTGGGTTTTACTCCCAACTGCTTTTTTATAGGATGTGATATTAGTGCTCAACTTATCAAAATTTGTAAAGAAAGGGGGCATGATGTTCTGGTGGCTGATGCAGTAAATCTTCCTTACAGAACTGGTTTTGGCGATGCAGCAATCTCTATAGCTGTATTACATCATCTAAGTACTGAGAACAGGAGGAAGAAAGCAATTGAAGAATTAATACGTGTTGTTAAAAAAGGTGGCCTAGTTTTAATTACAGTGTGGGCTGTTGAACAGGAGGATAAATCTTTATTGACGAAATGGATGCCACTTACAGAGAAGTATGTTGAAGAGTGGGTTGGACCAGGTATTCATCTAAGGGATTCCAAGGAGAATTTCACTGGAAGTATGCCAGAAAATAAATCTCTACCATCTCAAAGTGGAGACGAGTTGCCGAGTTGTATTGATGAAAACTTATCAAAGACTGCCCAGCAAGAATATTTTGTACCATGGCACTTACCTTATCATCGTGCAGAAGTCAGCGGCACGTCTGCCAGTGCCCTTGCCAGTGGTTTAGCAAAGAAAGATGATAAAAAGGCTGCTGTTGTGTATAACAGATACTATCATGTTTTCAGTGAAGGCGAGCTTGAAAGGCTAGTTTCTGGTATGGACAACGCAGTAGTGATCGATCGATTTTATGACAAGTCAAACTGGTGTATAGTTCTTGAGAAAACAGTGGTATCCCTTGATGTTTATTGCTCTTGCTATCTCGGATCTGTTCTTGGCCAATCCTGGTTTGGAGATGGAGATCTGCAGGTTCCCTTGAAGAAAATGCATGCACTGTAA

Protein sequence

MPQACSRASRVSKFFYQNSKIFDLSSVVIGSGHHFRLFSAMKEIKVKAGSKSDPHLSNDETNAQQSSCANQGCSSNVQCTPEIEKKYVHRVYDAIAPHFSSTRFAKWPKVAAFLSSLPLGSLILDAGCGNGKYLGFTPNCFFIGCDISAQLIKICKERGHDVLVADAVNLPYRTGFGDAAISIAVLHHLSTENRRKKAIEELIRVVKKGGLVLITVWAVEQEDKSLLTKWMPLTEKYVEEWVGPGIHLRDSKENFTGSMPENKSLPSQSGDELPSCIDENLSKTAQQEYFVPWHLPYHRAEVSGTSASALASGLAKKDDKKAAVVYNRYYHVFSEGELERLVSGMDNAVVIDRFYDKSNWCIVLEKTVVSLDVYCSCYLGSVLGQSWFGDGDLQVPLKKMHAL
Homology
BLAST of Moc04g39510 vs. NCBI nr
Match: XP_022152525.1 (alkylated DNA repair protein alkB homolog 8 isoform X1 [Momordica charantia] >XP_022152526.1 alkylated DNA repair protein alkB homolog 8 isoform X1 [Momordica charantia])

HSP 1 Score: 734.6 bits (1895), Expect = 4.7e-208
Identity = 368/392 (93.88%), Postives = 368/392 (93.88%), Query Frame = 0

Query: 1   MPQACSRASRVSKFFYQNSKIFDLSSVVIGSGHHFRLFSAMKEIKVKAGSKSDPHLSNDE 60
           MPQACSRASRVSKFFYQNSKIFDLSSVVIGSGHHFRLFSAMKEIKVKAGSKSDPHLSNDE
Sbjct: 1   MPQACSRASRVSKFFYQNSKIFDLSSVVIGSGHHFRLFSAMKEIKVKAGSKSDPHLSNDE 60

Query: 61  TNAQQSSCANQGCSSNVQCTPEIEKKYVHRVYDAIAPHFSSTRFAKWPKVAAFLSSLPLG 120
           TNAQQSSCANQGCSSNVQCTPEIEKKYVHRVYDAIAPHFSSTRFAKWPKVAAFLSSLPLG
Sbjct: 61  TNAQQSSCANQGCSSNVQCTPEIEKKYVHRVYDAIAPHFSSTRFAKWPKVAAFLSSLPLG 120

Query: 121 SLILDAGCGNGKYLGFTPNCFFIGCDISAQLIKICKERGHDVLVADAVNLPYRTGFGDAA 180
           SLILDAGCGNGKYLGFTPNCFFIGCDISAQLIKICKERGHDVLVADAVNLPYRTGFGDAA
Sbjct: 121 SLILDAGCGNGKYLGFTPNCFFIGCDISAQLIKICKERGHDVLVADAVNLPYRTGFGDAA 180

Query: 181 ISIAVLHHLSTENRRKKAIEELIRVVKKGGLVLITVWAVEQEDKSLLTKWMPLTEKYVEE 240
           ISIAVLHHLSTENRRKKAIEELIRVVKKGGLVLITVWAVEQEDKSLLTKWMPLTEKYVEE
Sbjct: 181 ISIAVLHHLSTENRRKKAIEELIRVVKKGGLVLITVWAVEQEDKSLLTKWMPLTEKYVEE 240

Query: 241 WVGP------------------------GIHLRDSKENFTGSMPENKSLPSQSGDELPSC 300
           WVGP                        GIHLRDSKENFTGSMPENKSLPSQSGDELPSC
Sbjct: 241 WVGPGSPRVRSPSSLALESIPEMNENGSGIHLRDSKENFTGSMPENKSLPSQSGDELPSC 300

Query: 301 IDENLSKTAQQEYFVPWHLPYHRAEVSGTSASALASGLAKKDDKKAAVVYNRYYHVFSEG 360
           IDENLSKTAQQEYFVPWHLPYHRAEVSGTSASALASGLAKKDDKKAAVVYNRYYHVFSEG
Sbjct: 301 IDENLSKTAQQEYFVPWHLPYHRAEVSGTSASALASGLAKKDDKKAAVVYNRYYHVFSEG 360

Query: 361 ELERLVSGMDNAVVIDRFYDKSNWCIVLEKTV 369
           ELERLVSGMDNAVVIDRFYDKSNWCIVLEKTV
Sbjct: 361 ELERLVSGMDNAVVIDRFYDKSNWCIVLEKTV 392

BLAST of Moc04g39510 vs. NCBI nr
Match: XP_022152527.1 (alkylated DNA repair protein alkB homolog 8 isoform X2 [Momordica charantia])

HSP 1 Score: 656.0 bits (1691), Expect = 2.1e-184
Identity = 328/352 (93.18%), Postives = 328/352 (93.18%), Query Frame = 0

Query: 41  MKEIKVKAGSKSDPHLSNDETNAQQSSCANQGCSSNVQCTPEIEKKYVHRVYDAIAPHFS 100
           MKEIKVKAGSKSDPHLSNDETNAQQSSCANQGCSSNVQCTPEIEKKYVHRVYDAIAPHFS
Sbjct: 1   MKEIKVKAGSKSDPHLSNDETNAQQSSCANQGCSSNVQCTPEIEKKYVHRVYDAIAPHFS 60

Query: 101 STRFAKWPKVAAFLSSLPLGSLILDAGCGNGKYLGFTPNCFFIGCDISAQLIKICKERGH 160
           STRFAKWPKVAAFLSSLPLGSLILDAGCGNGKYLGFTPNCFFIGCDISAQLIKICKERGH
Sbjct: 61  STRFAKWPKVAAFLSSLPLGSLILDAGCGNGKYLGFTPNCFFIGCDISAQLIKICKERGH 120

Query: 161 DVLVADAVNLPYRTGFGDAAISIAVLHHLSTENRRKKAIEELIRVVKKGGLVLITVWAVE 220
           DVLVADAVNLPYRTGFGDAAISIAVLHHLSTENRRKKAIEELIRVVKKGGLVLITVWAVE
Sbjct: 121 DVLVADAVNLPYRTGFGDAAISIAVLHHLSTENRRKKAIEELIRVVKKGGLVLITVWAVE 180

Query: 221 QEDKSLLTKWMPLTEKYVEEWVGP------------------------GIHLRDSKENFT 280
           QEDKSLLTKWMPLTEKYVEEWVGP                        GIHLRDSKENFT
Sbjct: 181 QEDKSLLTKWMPLTEKYVEEWVGPGSPRVRSPSSLALESIPEMNENGSGIHLRDSKENFT 240

Query: 281 GSMPENKSLPSQSGDELPSCIDENLSKTAQQEYFVPWHLPYHRAEVSGTSASALASGLAK 340
           GSMPENKSLPSQSGDELPSCIDENLSKTAQQEYFVPWHLPYHRAEVSGTSASALASGLAK
Sbjct: 241 GSMPENKSLPSQSGDELPSCIDENLSKTAQQEYFVPWHLPYHRAEVSGTSASALASGLAK 300

Query: 341 KDDKKAAVVYNRYYHVFSEGELERLVSGMDNAVVIDRFYDKSNWCIVLEKTV 369
           KDDKKAAVVYNRYYHVFSEGELERLVSGMDNAVVIDRFYDKSNWCIVLEKTV
Sbjct: 301 KDDKKAAVVYNRYYHVFSEGELERLVSGMDNAVVIDRFYDKSNWCIVLEKTV 352

BLAST of Moc04g39510 vs. NCBI nr
Match: XP_023518340.1 (alkylated DNA repair protein alkB homolog 8-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 635.2 bits (1637), Expect = 3.9e-178
Identity = 322/393 (81.93%), Postives = 341/393 (86.77%), Query Frame = 0

Query: 1   MPQACSRASRVSKFFYQNSKIFDLSSVVIGSGHHFRLFSAMKEIKVKAGSKSDPHLSNDE 60
           MPQA   ASRVSK F + SKIF LSSVV GSGHH RLFSAMKEIKVK GS +DPHL NDE
Sbjct: 1   MPQASYWASRVSKRFCRTSKIFGLSSVVSGSGHHCRLFSAMKEIKVKGGSNADPHLLNDE 60

Query: 61  TNAQQSSCANQGCS-SNVQCTPEIEKKYVHRVYDAIAPHFSSTRFAKWPKVAAFLSSLPL 120
            +A Q SCA QGCS SNVQCTPEIEKKYVHRVYDAIAPHFSSTRFAKWPKVA+FLSSLPL
Sbjct: 61  AHAPQLSCATQGCSASNVQCTPEIEKKYVHRVYDAIAPHFSSTRFAKWPKVASFLSSLPL 120

Query: 121 GSLILDAGCGNGKYLGFTPNCFFIGCDISAQLIKICKERGHDVLVADAVNLPYRTGFGDA 180
           GSL+LDAGCGNGKYLGF PNCFFIGCDISAQLIKIC ERGH+VLVADAVNLPYRTGFGDA
Sbjct: 121 GSLVLDAGCGNGKYLGFNPNCFFIGCDISAQLIKICDERGHEVLVADAVNLPYRTGFGDA 180

Query: 181 AISIAVLHHLSTENRRKKAIEELIRVVKKGGLVLITVWAVEQEDKSLLTKWMPLTEKYVE 240
           AISIAVLHHLSTENRR+KAIEELIRVVKKGGLVLITVWAVEQEDKSLLTKWMPL+EKYV+
Sbjct: 181 AISIAVLHHLSTENRRRKAIEELIRVVKKGGLVLITVWAVEQEDKSLLTKWMPLSEKYVD 240

Query: 241 EWVGP------------------------GIHLRDSKENFTGSMPENKSLPSQSGDELPS 300
           EWVGP                        G+ L+DSKENFTGS PENK LPS+SG++  +
Sbjct: 241 EWVGPGSPRVRSPSSLALESIPEMNENHAGVSLKDSKENFTGSKPENKPLPSRSGNDFMN 300

Query: 301 CIDENLSKTAQQEYFVPWHLPYHRAEVSGTSASALASGLAKKDDKKAAVVYNRYYHVFSE 360
           C DENL K  QQEYFVPWHLPYHRAEVSG+SASALASGLAKKDDKKAAVVYNRYYHVFSE
Sbjct: 301 CNDENLLK-IQQEYFVPWHLPYHRAEVSGSSASALASGLAKKDDKKAAVVYNRYYHVFSE 360

Query: 361 GELERLVSGMDNAVVIDRFYDKSNWCIVLEKTV 369
           GELERLVSGMD+AVV+DRFYDKSNWC+VLEKTV
Sbjct: 361 GELERLVSGMDDAVVVDRFYDKSNWCVVLEKTV 392

BLAST of Moc04g39510 vs. NCBI nr
Match: XP_022939521.1 (alkylated DNA repair protein alkB homolog 8-like isoform X1 [Cucurbita moschata])

HSP 1 Score: 631.3 bits (1627), Expect = 5.6e-177
Identity = 324/393 (82.44%), Postives = 338/393 (86.01%), Query Frame = 0

Query: 1   MPQACSRASRVSKFFYQNSKIFDLSSVVIGSGHHFRLFSAMKEIKVKAGSKSDPHLSNDE 60
           MPQA   ASRVSK F + SKIF LSSVV GSGHH RLFSAMKEIKVK GS +DPHL NDE
Sbjct: 54  MPQAFYWASRVSKRFCRTSKIFGLSSVVSGSGHHCRLFSAMKEIKVKGGSNADPHLLNDE 113

Query: 61  TNAQQSSCANQGCS-SNVQCTPEIEKKYVHRVYDAIAPHFSSTRFAKWPKVAAFLSSLPL 120
            +A Q SCANQGCS SNVQCTPEIEKKYVHRVYDAIAPHFSSTRFAKWPKVA+FLSSLP 
Sbjct: 114 AHAPQLSCANQGCSASNVQCTPEIEKKYVHRVYDAIAPHFSSTRFAKWPKVASFLSSLPR 173

Query: 121 GSLILDAGCGNGKYLGFTPNCFFIGCDISAQLIKICKERGHDVLVADAVNLPYRTGFGDA 180
           GSLILDAGCGNGKYLGF  NCFFIGCDIS QLIKIC ERGH+VLVADAVNLPYRTGFGDA
Sbjct: 174 GSLILDAGCGNGKYLGFNLNCFFIGCDISPQLIKICNERGHEVLVADAVNLPYRTGFGDA 233

Query: 181 AISIAVLHHLSTENRRKKAIEELIRVVKKGGLVLITVWAVEQEDKSLLTKWMPLTEKYVE 240
           AISIAVLHHLSTENRRKKAIEEL+RVVKKGGLVLITVWAVEQEDKSLLTKWMPL+EKYVE
Sbjct: 234 AISIAVLHHLSTENRRKKAIEELLRVVKKGGLVLITVWAVEQEDKSLLTKWMPLSEKYVE 293

Query: 241 EWVGP------------------------GIHLRDSKENFTGSMPENKSLPSQSGDELPS 300
           EWVGP                        G +L+DSK NFTGSMP N  LPSQSG++L +
Sbjct: 294 EWVGPGSPRVRSPSSLALESIPEMNENNSGTYLKDSKVNFTGSMPGNIPLPSQSGNDLAN 353

Query: 301 CIDENLSKTAQQEYFVPWHLPYHRAEVSGTSASALASGLAKKDDKKAAVVYNRYYHVFSE 360
           C DENL KT QQEYFVPWHLPYHRAEVSGTSA ALASGLAKKDDKKAAVVYNRYYHVFSE
Sbjct: 354 CNDENLWKT-QQEYFVPWHLPYHRAEVSGTSAGALASGLAKKDDKKAAVVYNRYYHVFSE 413

Query: 361 GELERLVSGMDNAVVIDRFYDKSNWCIVLEKTV 369
           GELERLVSGMDNAVV+DRFYDKSNWCIVLEKT+
Sbjct: 414 GELERLVSGMDNAVVVDRFYDKSNWCIVLEKTM 445

BLAST of Moc04g39510 vs. NCBI nr
Match: XP_022939523.1 (alkylated DNA repair protein alkB homolog 8-like isoform X2 [Cucurbita moschata])

HSP 1 Score: 631.3 bits (1627), Expect = 5.6e-177
Identity = 324/393 (82.44%), Postives = 338/393 (86.01%), Query Frame = 0

Query: 1   MPQACSRASRVSKFFYQNSKIFDLSSVVIGSGHHFRLFSAMKEIKVKAGSKSDPHLSNDE 60
           MPQA   ASRVSK F + SKIF LSSVV GSGHH RLFSAMKEIKVK GS +DPHL NDE
Sbjct: 1   MPQAFYWASRVSKRFCRTSKIFGLSSVVSGSGHHCRLFSAMKEIKVKGGSNADPHLLNDE 60

Query: 61  TNAQQSSCANQGCS-SNVQCTPEIEKKYVHRVYDAIAPHFSSTRFAKWPKVAAFLSSLPL 120
            +A Q SCANQGCS SNVQCTPEIEKKYVHRVYDAIAPHFSSTRFAKWPKVA+FLSSLP 
Sbjct: 61  AHAPQLSCANQGCSASNVQCTPEIEKKYVHRVYDAIAPHFSSTRFAKWPKVASFLSSLPR 120

Query: 121 GSLILDAGCGNGKYLGFTPNCFFIGCDISAQLIKICKERGHDVLVADAVNLPYRTGFGDA 180
           GSLILDAGCGNGKYLGF  NCFFIGCDIS QLIKIC ERGH+VLVADAVNLPYRTGFGDA
Sbjct: 121 GSLILDAGCGNGKYLGFNLNCFFIGCDISPQLIKICNERGHEVLVADAVNLPYRTGFGDA 180

Query: 181 AISIAVLHHLSTENRRKKAIEELIRVVKKGGLVLITVWAVEQEDKSLLTKWMPLTEKYVE 240
           AISIAVLHHLSTENRRKKAIEEL+RVVKKGGLVLITVWAVEQEDKSLLTKWMPL+EKYVE
Sbjct: 181 AISIAVLHHLSTENRRKKAIEELLRVVKKGGLVLITVWAVEQEDKSLLTKWMPLSEKYVE 240

Query: 241 EWVGP------------------------GIHLRDSKENFTGSMPENKSLPSQSGDELPS 300
           EWVGP                        G +L+DSK NFTGSMP N  LPSQSG++L +
Sbjct: 241 EWVGPGSPRVRSPSSLALESIPEMNENNSGTYLKDSKVNFTGSMPGNIPLPSQSGNDLAN 300

Query: 301 CIDENLSKTAQQEYFVPWHLPYHRAEVSGTSASALASGLAKKDDKKAAVVYNRYYHVFSE 360
           C DENL KT QQEYFVPWHLPYHRAEVSGTSA ALASGLAKKDDKKAAVVYNRYYHVFSE
Sbjct: 301 CNDENLWKT-QQEYFVPWHLPYHRAEVSGTSAGALASGLAKKDDKKAAVVYNRYYHVFSE 360

Query: 361 GELERLVSGMDNAVVIDRFYDKSNWCIVLEKTV 369
           GELERLVSGMDNAVV+DRFYDKSNWCIVLEKT+
Sbjct: 361 GELERLVSGMDNAVVVDRFYDKSNWCIVLEKTM 392

BLAST of Moc04g39510 vs. ExPASy Swiss-Prot
Match: Q94A09 (tRNA (carboxymethyluridine(34)-5-O)-methyltransferase OS=Arabidopsis thaliana OX=3702 GN=TRM9 PE=1 SV=1)

HSP 1 Score: 452.6 bits (1163), Expect = 4.7e-126
Identity = 240/386 (62.18%), Postives = 279/386 (72.28%), Query Frame = 0

Query: 31  SGHHFRLFSAMKEIKVKAGSKSDPHLSNDETNAQQSSCANQGCSSNVQCTPEIEKKYVHR 90
           SG +    S M++IKVK+ SK     S++E  +     ++    S+V+ TPEIEKKYVHR
Sbjct: 19  SGFYSISTSIMRDIKVKSDSKEFLTSSDEEEESVSIRVSSSSSLSSVKSTPEIEKKYVHR 78

Query: 91  VYDAIAPHFSSTRFAKWPKVAAFLSSLPLGSLILDAGCGNGKYLGFTPNCFFIGCDISAQ 150
           VYDAIAPHFSSTRFAKWPKVAAFL SLP GS+ILDAGCGNGKYLG  P+CFFIGCDIS  
Sbjct: 79  VYDAIAPHFSSTRFAKWPKVAAFLESLPSGSVILDAGCGNGKYLGLNPSCFFIGCDISHP 138

Query: 151 LIKICKERGHDVLVADAVNLPYRTGFGDAAISIAVLHHLSTENRRKKAIEELIRVVKKGG 210
           LIKIC ++G +VLVADAVNLPYR  FGDAAISIAVLHHLSTENRRKKAIEEL+RVVK GG
Sbjct: 139 LIKICSDKGQEVLVADAVNLPYREEFGDAAISIAVLHHLSTENRRKKAIEELVRVVKPGG 198

Query: 211 LVLITVWAVEQEDKSLLTKWMPLTEKYVEEWVGPG-----------------------IH 270
            VLITVWA EQED SLLTKW PL+ KYVEEWVGPG                       + 
Sbjct: 199 FVLITVWAAEQEDTSLLTKWTPLSAKYVEEWVGPGSPMNSPRVRNNPFFSLESIPETEVS 258

Query: 271 LRDSK---ENFTG--SMPEN-KSLPSQSGDEL-------------PSCIDENLS--KTAQ 330
            ++ K     F G  S+PE+ +S   Q G+ +                ++E+L   K +Q
Sbjct: 259 TKEQKVENSQFIGLESIPESEESTREQKGESIIPETKASIVEQKDEKSVEESLEALKKSQ 318

Query: 331 QEYFVPWHLPYHRAEVSGTSASALASGLAKKDDKKAAVVYNRYYHVFSEGELERLVSGMD 373
           QEYFVPWHLPYHRAEVSG SASALASGLAKKDD+K AVVYNRYYHVFSEGELERL SG+ 
Sbjct: 319 QEYFVPWHLPYHRAEVSGASASALASGLAKKDDRKGAVVYNRYYHVFSEGELERLASGVG 378

BLAST of Moc04g39510 vs. ExPASy Swiss-Prot
Match: Q07G10 (Alkylated DNA repair protein alkB homolog 8 OS=Xenopus tropicalis OX=8364 GN=alkbh8 PE=2 SV=2)

HSP 1 Score: 233.0 bits (593), Expect = 5.8e-60
Identity = 120/285 (42.11%), Postives = 171/285 (60.00%), Query Frame = 0

Query: 83  IEKKYVHRVYDAIAPHFSSTRFAKWPKVAAFLSSLPLGSLILDAGCGNGKYLGFTPNCFF 142
           +E++YVH+VYD IA HFSSTR   WPK+  FL+SLP GSL+ D GCGNGKYLG   +   
Sbjct: 369 LEREYVHKVYDDIAGHFSSTRHTPWPKIGDFLASLPKGSLVADVGCGNGKYLGVNKDLCM 428

Query: 143 IGCDISAQLIKICKERGHDVLVADAVNLPYRTGFGDAAISIAVLHHLSTENRRKKAIEEL 202
           IGCD S  L+ IC+ER  +  V DA+++P+R G  DA ISIAV+HH +TE RR  A++EL
Sbjct: 429 IGCDRSKNLVDICRERTFEAFVCDALSVPFRAGAFDACISIAVIHHFATEERRIAALQEL 488

Query: 203 IRVVKKGGLVLITVWAVEQEDKSLLTKWMPLTEKYVEEWVGPGIHLRDSKENFTGSMPEN 262
           IR+++KGG  LI VWA+EQE K   +K++                    KE+ T   P +
Sbjct: 489 IRLLRKGGKALIYVWALEQEYKKNKSKYL--------------------KESKTSQGPSS 548

Query: 263 KSLPSQSGDELPSCIDENLSKTAQQEYFVPWHL-PYHRAEVSGTSASALASGLAKKDDKK 322
            +L +    + P  I  N +    Q+  VPWHL P ++++V+  +          + +  
Sbjct: 549 DALGTNLAVKAPLPIHTNRTSFHSQDLLVPWHLKPTNKSKVTPENKE------QNEKEHG 608

Query: 323 AAVVYNRYYHVFSEGELERLVSGMDNAVVIDRFYDKSNWCIVLEK 367
              VY+R+YHVF EGELE + + + N  V   ++D+ NWC++LEK
Sbjct: 609 PDSVYHRFYHVFCEGELEAMCNRLSNVAVQHSYHDQGNWCVILEK 627

BLAST of Moc04g39510 vs. ExPASy Swiss-Prot
Match: Q80Y20 (Alkylated DNA repair protein alkB homolog 8 OS=Mus musculus OX=10090 GN=Alkbh8 PE=1 SV=1)

HSP 1 Score: 219.2 bits (557), Expect = 8.7e-56
Identity = 118/303 (38.94%), Postives = 177/303 (58.42%), Query Frame = 0

Query: 82  EIEKKYVHRVYDAIAPHFSSTRFAKWPKVAAFLSSLPLGSLILDAGCGNGKYLGFTPNCF 141
           E+E+K+VH+VY+ IA HFSSTR + WP++  FL +LP GS++ D GCGNGKYLG   + +
Sbjct: 369 ELEQKHVHQVYNEIASHFSSTRHSPWPRIVEFLKALPSGSIVADIGCGNGKYLGINKDLY 428

Query: 142 FIGCDISAQLIKICKERGHDVLVADAVNLPYRTGFGDAAISIAVLHHLSTENRRKKAIEE 201
            IGCD S  L+ IC+ER    LV DA+ +P R+G  DA ISIAV+HH +T  RR +A++E
Sbjct: 429 MIGCDRSQNLVDICRERQFQALVCDALAVPVRSGSCDACISIAVIHHFATAERRVEALQE 488

Query: 202 LIRVVKKGGLVLITVWAVEQEDKSLLTKWM----------------PLTEKYVEEWVGPG 261
           L R+++ GG  LI VWA+EQE K+  +K++                  TE+++      G
Sbjct: 489 LARLLRPGGQALIYVWAMEQEYKNQKSKYLRGKRISQGDKDELNSATSTEEFLVNQTPEG 548

Query: 262 IHLRDSKENFTGSMPENKSLPSQS--GDELPSCIDENLSKTAQQEYFVPWHLPYHRAEVS 321
           ++   +    + S+ + +   S+     ELP  I  N +    Q+  VPWHL  +     
Sbjct: 549 VNEDPALSVNSSSITKEEEYKSRKVPNSELP--IHINRTCFHSQDVLVPWHLKRN----P 608

Query: 322 GTSASALASGLAKKDDKKAAVVYNRYYHVFSEGELERLVSGMDNAVVIDRFYDKSNWCIV 367
           G   +   SG+A   D   + V++RYYHVF +GELE     + +  ++  +YD+ NWC+V
Sbjct: 609 GKDKAIEPSGVAGCPD--PSPVFHRYYHVFCDGELEASCQAVGDVSILQSYYDQGNWCVV 663

BLAST of Moc04g39510 vs. ExPASy Swiss-Prot
Match: A1A4L5 (Alkylated DNA repair protein alkB homolog 8 OS=Bos taurus OX=9913 GN=ALKBH8 PE=2 SV=1)

HSP 1 Score: 209.1 bits (531), Expect = 9.0e-53
Identity = 117/326 (35.89%), Postives = 175/326 (53.68%), Query Frame = 0

Query: 73  CSSNVQCTP-----------EIEKKYVHRVYDAIAPHFSSTRFAKWPKVAAFLSSLPLGS 132
           C S ++ TP           ++E+ YVHRVY+ IA HFSSTR   WP +  FL +LP GS
Sbjct: 349 CDSQMKETPASFPESDKEALQLEQDYVHRVYEEIARHFSSTRHTPWPHIVEFLKALPSGS 408

Query: 133 LILDAGCGNGKYLGFTPNCFFIGCDISAQLIKICKERGHDVLVADAVNLPYRTGFGDAAI 192
           L+ D GCGNGKYLG     + IGCD S  L+ IC+ER +   V DA+ +P R+G  DA I
Sbjct: 409 LVADIGCGNGKYLGINNELYMIGCDHSQNLVDICRERQYQAFVCDALAVPIRSGSCDACI 468

Query: 193 SIAVLHHLSTENRRKKAIEELIRVVKKGGLVLITVWAVEQEDKSLLTKWM--PLTEKYVE 252
           SIAV+HH +T  RR  A++EL+R+++ GG  LI VWA+EQE     +K++    T + ++
Sbjct: 469 SIAVIHHFATAERRVAALQELVRLLRPGGKALIYVWAMEQEYNKKKSKYLRENRTSQGMK 528

Query: 253 EWVGPGIHLRD---SKENFTGSMPENKSLPSQSGDELPSCIDENLSKTA----------- 312
           E +     +++    +    G+     S+ S +  +   C  +N++ +            
Sbjct: 529 EEISNDASVQELLVKQLPNVGNQDSAHSVSSINDFQDGGCNSKNVANSKLPIHTNRTSFH 588

Query: 313 QQEYFVPWHLPYH-----RAEVSGTSASALASGLAKKDDKKAAVVYNRYYHVFSEGELER 367
            Q+  VPWH   +     R E  G   S           +    V++RYYHVF EGELE 
Sbjct: 589 SQDLLVPWHFKGNPGKDKRVEPFGPLGS-----------RDPGPVFHRYYHVFCEGELEA 648

BLAST of Moc04g39510 vs. ExPASy Swiss-Prot
Match: Q95K79 (Alkylated DNA repair protein alkB homolog 8 OS=Macaca fascicularis OX=9541 GN=ALKBH8 PE=2 SV=1)

HSP 1 Score: 205.7 bits (522), Expect = 1.0e-51
Identity = 112/300 (37.33%), Postives = 158/300 (52.67%), Query Frame = 0

Query: 83  IEKKYVHRVYDAIAPHFSSTRFAKWPKVAAFLSSLPLGSLILDAGCGNGKYLGFTPNCFF 142
           +E++YVH+VY+ IA HFSSTR   WP +  FL +LP GS++ D GCGNGKYLG     + 
Sbjct: 370 LEQEYVHQVYEEIAGHFSSTRHTPWPHIVEFLKALPSGSIVADIGCGNGKYLGVNKELYM 429

Query: 143 IGCDISAQLIKICKERGHDVLVADAVNLPYRTGFGDAAISIAVLHHLSTENRRKKAIEEL 202
           +GCD S  L+ IC+ER     V DA+ +P R+G  DA ISIAV+HH +T  RR  A++E+
Sbjct: 430 VGCDRSQNLVDICRERQFQAFVCDALAVPVRSGSCDACISIAVIHHFATAERRVAALQEI 489

Query: 203 IRVVKKGGLVLITVWAVEQE---DKSLLTKWMPLTEKYVEEWVGPGIHLRDSKENF--TG 262
           +R+++ GG  LI VWA+EQE    KS   K    ++   EE        R   E     G
Sbjct: 490 VRLLRPGGKALIYVWAMEQEYNKQKSKYLKGNRNSQGKKEEMNSDTSVQRSLVEQMPDMG 549

Query: 263 SMPENKSLPSQSGDELPSC-----------IDENLSKTAQQEYFVPWHLPYHRAEVSGTS 322
           S     S+P  +  +   C           I  N +    Q+  VPWHL           
Sbjct: 550 SRDSASSVPRINDSQEGGCNSRQVSNSKLPIHVNRTSFYSQDMLVPWHLK------GNPD 609

Query: 323 ASALASGLAKKDDKKAAVVYNRYYHVFSEGELERLVSGMDNAVVIDRFYDKSNWCIVLEK 367
                        +  + V++RYYHVF EGELE L   + +  ++  +YD+ NWC++L+K
Sbjct: 610 KGKPVEPFGPIGSQDPSPVFHRYYHVFREGELEALCRTVSDVRILQSYYDQGNWCVILQK 663

BLAST of Moc04g39510 vs. ExPASy TrEMBL
Match: A0A6J1DE66 (alkylated DNA repair protein alkB homolog 8 isoform X1 OS=Momordica charantia OX=3673 GN=LOC111020230 PE=4 SV=1)

HSP 1 Score: 734.6 bits (1895), Expect = 2.3e-208
Identity = 368/392 (93.88%), Postives = 368/392 (93.88%), Query Frame = 0

Query: 1   MPQACSRASRVSKFFYQNSKIFDLSSVVIGSGHHFRLFSAMKEIKVKAGSKSDPHLSNDE 60
           MPQACSRASRVSKFFYQNSKIFDLSSVVIGSGHHFRLFSAMKEIKVKAGSKSDPHLSNDE
Sbjct: 1   MPQACSRASRVSKFFYQNSKIFDLSSVVIGSGHHFRLFSAMKEIKVKAGSKSDPHLSNDE 60

Query: 61  TNAQQSSCANQGCSSNVQCTPEIEKKYVHRVYDAIAPHFSSTRFAKWPKVAAFLSSLPLG 120
           TNAQQSSCANQGCSSNVQCTPEIEKKYVHRVYDAIAPHFSSTRFAKWPKVAAFLSSLPLG
Sbjct: 61  TNAQQSSCANQGCSSNVQCTPEIEKKYVHRVYDAIAPHFSSTRFAKWPKVAAFLSSLPLG 120

Query: 121 SLILDAGCGNGKYLGFTPNCFFIGCDISAQLIKICKERGHDVLVADAVNLPYRTGFGDAA 180
           SLILDAGCGNGKYLGFTPNCFFIGCDISAQLIKICKERGHDVLVADAVNLPYRTGFGDAA
Sbjct: 121 SLILDAGCGNGKYLGFTPNCFFIGCDISAQLIKICKERGHDVLVADAVNLPYRTGFGDAA 180

Query: 181 ISIAVLHHLSTENRRKKAIEELIRVVKKGGLVLITVWAVEQEDKSLLTKWMPLTEKYVEE 240
           ISIAVLHHLSTENRRKKAIEELIRVVKKGGLVLITVWAVEQEDKSLLTKWMPLTEKYVEE
Sbjct: 181 ISIAVLHHLSTENRRKKAIEELIRVVKKGGLVLITVWAVEQEDKSLLTKWMPLTEKYVEE 240

Query: 241 WVGP------------------------GIHLRDSKENFTGSMPENKSLPSQSGDELPSC 300
           WVGP                        GIHLRDSKENFTGSMPENKSLPSQSGDELPSC
Sbjct: 241 WVGPGSPRVRSPSSLALESIPEMNENGSGIHLRDSKENFTGSMPENKSLPSQSGDELPSC 300

Query: 301 IDENLSKTAQQEYFVPWHLPYHRAEVSGTSASALASGLAKKDDKKAAVVYNRYYHVFSEG 360
           IDENLSKTAQQEYFVPWHLPYHRAEVSGTSASALASGLAKKDDKKAAVVYNRYYHVFSEG
Sbjct: 301 IDENLSKTAQQEYFVPWHLPYHRAEVSGTSASALASGLAKKDDKKAAVVYNRYYHVFSEG 360

Query: 361 ELERLVSGMDNAVVIDRFYDKSNWCIVLEKTV 369
           ELERLVSGMDNAVVIDRFYDKSNWCIVLEKTV
Sbjct: 361 ELERLVSGMDNAVVIDRFYDKSNWCIVLEKTV 392

BLAST of Moc04g39510 vs. ExPASy TrEMBL
Match: A0A6J1DGH6 (alkylated DNA repair protein alkB homolog 8 isoform X2 OS=Momordica charantia OX=3673 GN=LOC111020230 PE=4 SV=1)

HSP 1 Score: 656.0 bits (1691), Expect = 1.0e-184
Identity = 328/352 (93.18%), Postives = 328/352 (93.18%), Query Frame = 0

Query: 41  MKEIKVKAGSKSDPHLSNDETNAQQSSCANQGCSSNVQCTPEIEKKYVHRVYDAIAPHFS 100
           MKEIKVKAGSKSDPHLSNDETNAQQSSCANQGCSSNVQCTPEIEKKYVHRVYDAIAPHFS
Sbjct: 1   MKEIKVKAGSKSDPHLSNDETNAQQSSCANQGCSSNVQCTPEIEKKYVHRVYDAIAPHFS 60

Query: 101 STRFAKWPKVAAFLSSLPLGSLILDAGCGNGKYLGFTPNCFFIGCDISAQLIKICKERGH 160
           STRFAKWPKVAAFLSSLPLGSLILDAGCGNGKYLGFTPNCFFIGCDISAQLIKICKERGH
Sbjct: 61  STRFAKWPKVAAFLSSLPLGSLILDAGCGNGKYLGFTPNCFFIGCDISAQLIKICKERGH 120

Query: 161 DVLVADAVNLPYRTGFGDAAISIAVLHHLSTENRRKKAIEELIRVVKKGGLVLITVWAVE 220
           DVLVADAVNLPYRTGFGDAAISIAVLHHLSTENRRKKAIEELIRVVKKGGLVLITVWAVE
Sbjct: 121 DVLVADAVNLPYRTGFGDAAISIAVLHHLSTENRRKKAIEELIRVVKKGGLVLITVWAVE 180

Query: 221 QEDKSLLTKWMPLTEKYVEEWVGP------------------------GIHLRDSKENFT 280
           QEDKSLLTKWMPLTEKYVEEWVGP                        GIHLRDSKENFT
Sbjct: 181 QEDKSLLTKWMPLTEKYVEEWVGPGSPRVRSPSSLALESIPEMNENGSGIHLRDSKENFT 240

Query: 281 GSMPENKSLPSQSGDELPSCIDENLSKTAQQEYFVPWHLPYHRAEVSGTSASALASGLAK 340
           GSMPENKSLPSQSGDELPSCIDENLSKTAQQEYFVPWHLPYHRAEVSGTSASALASGLAK
Sbjct: 241 GSMPENKSLPSQSGDELPSCIDENLSKTAQQEYFVPWHLPYHRAEVSGTSASALASGLAK 300

Query: 341 KDDKKAAVVYNRYYHVFSEGELERLVSGMDNAVVIDRFYDKSNWCIVLEKTV 369
           KDDKKAAVVYNRYYHVFSEGELERLVSGMDNAVVIDRFYDKSNWCIVLEKTV
Sbjct: 301 KDDKKAAVVYNRYYHVFSEGELERLVSGMDNAVVIDRFYDKSNWCIVLEKTV 352

BLAST of Moc04g39510 vs. ExPASy TrEMBL
Match: A0A6J1FLV5 (alkylated DNA repair protein alkB homolog 8-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111445406 PE=4 SV=1)

HSP 1 Score: 631.3 bits (1627), Expect = 2.7e-177
Identity = 324/393 (82.44%), Postives = 338/393 (86.01%), Query Frame = 0

Query: 1   MPQACSRASRVSKFFYQNSKIFDLSSVVIGSGHHFRLFSAMKEIKVKAGSKSDPHLSNDE 60
           MPQA   ASRVSK F + SKIF LSSVV GSGHH RLFSAMKEIKVK GS +DPHL NDE
Sbjct: 1   MPQAFYWASRVSKRFCRTSKIFGLSSVVSGSGHHCRLFSAMKEIKVKGGSNADPHLLNDE 60

Query: 61  TNAQQSSCANQGCS-SNVQCTPEIEKKYVHRVYDAIAPHFSSTRFAKWPKVAAFLSSLPL 120
            +A Q SCANQGCS SNVQCTPEIEKKYVHRVYDAIAPHFSSTRFAKWPKVA+FLSSLP 
Sbjct: 61  AHAPQLSCANQGCSASNVQCTPEIEKKYVHRVYDAIAPHFSSTRFAKWPKVASFLSSLPR 120

Query: 121 GSLILDAGCGNGKYLGFTPNCFFIGCDISAQLIKICKERGHDVLVADAVNLPYRTGFGDA 180
           GSLILDAGCGNGKYLGF  NCFFIGCDIS QLIKIC ERGH+VLVADAVNLPYRTGFGDA
Sbjct: 121 GSLILDAGCGNGKYLGFNLNCFFIGCDISPQLIKICNERGHEVLVADAVNLPYRTGFGDA 180

Query: 181 AISIAVLHHLSTENRRKKAIEELIRVVKKGGLVLITVWAVEQEDKSLLTKWMPLTEKYVE 240
           AISIAVLHHLSTENRRKKAIEEL+RVVKKGGLVLITVWAVEQEDKSLLTKWMPL+EKYVE
Sbjct: 181 AISIAVLHHLSTENRRKKAIEELLRVVKKGGLVLITVWAVEQEDKSLLTKWMPLSEKYVE 240

Query: 241 EWVGP------------------------GIHLRDSKENFTGSMPENKSLPSQSGDELPS 300
           EWVGP                        G +L+DSK NFTGSMP N  LPSQSG++L +
Sbjct: 241 EWVGPGSPRVRSPSSLALESIPEMNENNSGTYLKDSKVNFTGSMPGNIPLPSQSGNDLAN 300

Query: 301 CIDENLSKTAQQEYFVPWHLPYHRAEVSGTSASALASGLAKKDDKKAAVVYNRYYHVFSE 360
           C DENL KT QQEYFVPWHLPYHRAEVSGTSA ALASGLAKKDDKKAAVVYNRYYHVFSE
Sbjct: 301 CNDENLWKT-QQEYFVPWHLPYHRAEVSGTSAGALASGLAKKDDKKAAVVYNRYYHVFSE 360

Query: 361 GELERLVSGMDNAVVIDRFYDKSNWCIVLEKTV 369
           GELERLVSGMDNAVV+DRFYDKSNWCIVLEKT+
Sbjct: 361 GELERLVSGMDNAVVVDRFYDKSNWCIVLEKTM 392

BLAST of Moc04g39510 vs. ExPASy TrEMBL
Match: A0A6J1FG55 (alkylated DNA repair protein alkB homolog 8-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111445406 PE=4 SV=1)

HSP 1 Score: 631.3 bits (1627), Expect = 2.7e-177
Identity = 324/393 (82.44%), Postives = 338/393 (86.01%), Query Frame = 0

Query: 1   MPQACSRASRVSKFFYQNSKIFDLSSVVIGSGHHFRLFSAMKEIKVKAGSKSDPHLSNDE 60
           MPQA   ASRVSK F + SKIF LSSVV GSGHH RLFSAMKEIKVK GS +DPHL NDE
Sbjct: 54  MPQAFYWASRVSKRFCRTSKIFGLSSVVSGSGHHCRLFSAMKEIKVKGGSNADPHLLNDE 113

Query: 61  TNAQQSSCANQGCS-SNVQCTPEIEKKYVHRVYDAIAPHFSSTRFAKWPKVAAFLSSLPL 120
            +A Q SCANQGCS SNVQCTPEIEKKYVHRVYDAIAPHFSSTRFAKWPKVA+FLSSLP 
Sbjct: 114 AHAPQLSCANQGCSASNVQCTPEIEKKYVHRVYDAIAPHFSSTRFAKWPKVASFLSSLPR 173

Query: 121 GSLILDAGCGNGKYLGFTPNCFFIGCDISAQLIKICKERGHDVLVADAVNLPYRTGFGDA 180
           GSLILDAGCGNGKYLGF  NCFFIGCDIS QLIKIC ERGH+VLVADAVNLPYRTGFGDA
Sbjct: 174 GSLILDAGCGNGKYLGFNLNCFFIGCDISPQLIKICNERGHEVLVADAVNLPYRTGFGDA 233

Query: 181 AISIAVLHHLSTENRRKKAIEELIRVVKKGGLVLITVWAVEQEDKSLLTKWMPLTEKYVE 240
           AISIAVLHHLSTENRRKKAIEEL+RVVKKGGLVLITVWAVEQEDKSLLTKWMPL+EKYVE
Sbjct: 234 AISIAVLHHLSTENRRKKAIEELLRVVKKGGLVLITVWAVEQEDKSLLTKWMPLSEKYVE 293

Query: 241 EWVGP------------------------GIHLRDSKENFTGSMPENKSLPSQSGDELPS 300
           EWVGP                        G +L+DSK NFTGSMP N  LPSQSG++L +
Sbjct: 294 EWVGPGSPRVRSPSSLALESIPEMNENNSGTYLKDSKVNFTGSMPGNIPLPSQSGNDLAN 353

Query: 301 CIDENLSKTAQQEYFVPWHLPYHRAEVSGTSASALASGLAKKDDKKAAVVYNRYYHVFSE 360
           C DENL KT QQEYFVPWHLPYHRAEVSGTSA ALASGLAKKDDKKAAVVYNRYYHVFSE
Sbjct: 354 CNDENLWKT-QQEYFVPWHLPYHRAEVSGTSAGALASGLAKKDDKKAAVVYNRYYHVFSE 413

Query: 361 GELERLVSGMDNAVVIDRFYDKSNWCIVLEKTV 369
           GELERLVSGMDNAVV+DRFYDKSNWCIVLEKT+
Sbjct: 414 GELERLVSGMDNAVVVDRFYDKSNWCIVLEKTM 445

BLAST of Moc04g39510 vs. ExPASy TrEMBL
Match: A0A6J1JY21 (alkylated DNA repair protein alkB homolog 8-like isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111489322 PE=4 SV=1)

HSP 1 Score: 627.1 bits (1616), Expect = 5.1e-176
Identity = 322/393 (81.93%), Postives = 335/393 (85.24%), Query Frame = 0

Query: 1   MPQACSRASRVSKFFYQNSKIFDLSSVVIGSGHHFRLFSAMKEIKVKAGSKSDPHLSNDE 60
           MPQA   ASRVSK F   SKIF LSSVV GS HH RLFSAMKEIKVK GS +DPHL NDE
Sbjct: 1   MPQAFYWASRVSKRFCHTSKIFGLSSVVSGSVHHCRLFSAMKEIKVKGGSNADPHLLNDE 60

Query: 61  TNAQQSSCANQGCS-SNVQCTPEIEKKYVHRVYDAIAPHFSSTRFAKWPKVAAFLSSLPL 120
            +A Q SCANQGCS SNVQCTPEIEKKYVHRVYDAIAPHFSSTRFAKWPKVA+FLSSLP 
Sbjct: 61  AHAPQLSCANQGCSASNVQCTPEIEKKYVHRVYDAIAPHFSSTRFAKWPKVASFLSSLPR 120

Query: 121 GSLILDAGCGNGKYLGFTPNCFFIGCDISAQLIKICKERGHDVLVADAVNLPYRTGFGDA 180
           GSLILDAGCGNGKYLGF  NCFFIGCDIS QLIKIC ERGH+VLVADAVNLPYRTGFGDA
Sbjct: 121 GSLILDAGCGNGKYLGFNLNCFFIGCDISPQLIKICNERGHEVLVADAVNLPYRTGFGDA 180

Query: 181 AISIAVLHHLSTENRRKKAIEELIRVVKKGGLVLITVWAVEQEDKSLLTKWMPLTEKYVE 240
           AISIAVLHHLSTENRRKKAIEEL+RVVKKGGLVLITVWAVEQEDKSLLTKWMPL+EKYVE
Sbjct: 181 AISIAVLHHLSTENRRKKAIEELLRVVKKGGLVLITVWAVEQEDKSLLTKWMPLSEKYVE 240

Query: 241 EWVGP------------------------GIHLRDSKENFTGSMPENKSLPSQSGDELPS 300
           EWVGP                        G +L+DSK NFTGSMPEN  LPSQ G++  +
Sbjct: 241 EWVGPGSPRVRSPSSLALESIPEMNENNSGTYLKDSKVNFTGSMPENIPLPSQGGNDFAN 300

Query: 301 CIDENLSKTAQQEYFVPWHLPYHRAEVSGTSASALASGLAKKDDKKAAVVYNRYYHVFSE 360
           C DENL KT QQEYFVPWHLPYHRAEVSGTSA ALASGLAKKDDKKAAVVYNRYYHVFSE
Sbjct: 301 CNDENLWKT-QQEYFVPWHLPYHRAEVSGTSAGALASGLAKKDDKKAAVVYNRYYHVFSE 360

Query: 361 GELERLVSGMDNAVVIDRFYDKSNWCIVLEKTV 369
           GELERLVSGMDNAVV+DRFYDKSNWCIVLEKT+
Sbjct: 361 GELERLVSGMDNAVVVDRFYDKSNWCIVLEKTM 392

BLAST of Moc04g39510 vs. TAIR 10
Match: AT1G36310.1 (S-adenosyl-L-methionine-dependent methyltransferases superfamily protein )

HSP 1 Score: 452.6 bits (1163), Expect = 3.3e-127
Identity = 240/386 (62.18%), Postives = 279/386 (72.28%), Query Frame = 0

Query: 31  SGHHFRLFSAMKEIKVKAGSKSDPHLSNDETNAQQSSCANQGCSSNVQCTPEIEKKYVHR 90
           SG +    S M++IKVK+ SK     S++E  +     ++    S+V+ TPEIEKKYVHR
Sbjct: 19  SGFYSISTSIMRDIKVKSDSKEFLTSSDEEEESVSIRVSSSSSLSSVKSTPEIEKKYVHR 78

Query: 91  VYDAIAPHFSSTRFAKWPKVAAFLSSLPLGSLILDAGCGNGKYLGFTPNCFFIGCDISAQ 150
           VYDAIAPHFSSTRFAKWPKVAAFL SLP GS+ILDAGCGNGKYLG  P+CFFIGCDIS  
Sbjct: 79  VYDAIAPHFSSTRFAKWPKVAAFLESLPSGSVILDAGCGNGKYLGLNPSCFFIGCDISHP 138

Query: 151 LIKICKERGHDVLVADAVNLPYRTGFGDAAISIAVLHHLSTENRRKKAIEELIRVVKKGG 210
           LIKIC ++G +VLVADAVNLPYR  FGDAAISIAVLHHLSTENRRKKAIEEL+RVVK GG
Sbjct: 139 LIKICSDKGQEVLVADAVNLPYREEFGDAAISIAVLHHLSTENRRKKAIEELVRVVKPGG 198

Query: 211 LVLITVWAVEQEDKSLLTKWMPLTEKYVEEWVGPG-----------------------IH 270
            VLITVWA EQED SLLTKW PL+ KYVEEWVGPG                       + 
Sbjct: 199 FVLITVWAAEQEDTSLLTKWTPLSAKYVEEWVGPGSPMNSPRVRNNPFFSLESIPETEVS 258

Query: 271 LRDSK---ENFTG--SMPEN-KSLPSQSGDEL-------------PSCIDENLS--KTAQ 330
            ++ K     F G  S+PE+ +S   Q G+ +                ++E+L   K +Q
Sbjct: 259 TKEQKVENSQFIGLESIPESEESTREQKGESIIPETKASIVEQKDEKSVEESLEALKKSQ 318

Query: 331 QEYFVPWHLPYHRAEVSGTSASALASGLAKKDDKKAAVVYNRYYHVFSEGELERLVSGMD 373
           QEYFVPWHLPYHRAEVSG SASALASGLAKKDD+K AVVYNRYYHVFSEGELERL SG+ 
Sbjct: 319 QEYFVPWHLPYHRAEVSGASASALASGLAKKDDRKGAVVYNRYYHVFSEGELERLASGVG 378

BLAST of Moc04g39510 vs. TAIR 10
Match: AT1G36310.2 (S-adenosyl-L-methionine-dependent methyltransferases superfamily protein )

HSP 1 Score: 452.6 bits (1163), Expect = 3.3e-127
Identity = 240/386 (62.18%), Postives = 279/386 (72.28%), Query Frame = 0

Query: 31  SGHHFRLFSAMKEIKVKAGSKSDPHLSNDETNAQQSSCANQGCSSNVQCTPEIEKKYVHR 90
           SG +    S M++IKVK+ SK     S++E  +     ++    S+V+ TPEIEKKYVHR
Sbjct: 19  SGFYSISTSIMRDIKVKSDSKEFLTSSDEEEESVSIRVSSSSSLSSVKSTPEIEKKYVHR 78

Query: 91  VYDAIAPHFSSTRFAKWPKVAAFLSSLPLGSLILDAGCGNGKYLGFTPNCFFIGCDISAQ 150
           VYDAIAPHFSSTRFAKWPKVAAFL SLP GS+ILDAGCGNGKYLG  P+CFFIGCDIS  
Sbjct: 79  VYDAIAPHFSSTRFAKWPKVAAFLESLPSGSVILDAGCGNGKYLGLNPSCFFIGCDISHP 138

Query: 151 LIKICKERGHDVLVADAVNLPYRTGFGDAAISIAVLHHLSTENRRKKAIEELIRVVKKGG 210
           LIKIC ++G +VLVADAVNLPYR  FGDAAISIAVLHHLSTENRRKKAIEEL+RVVK GG
Sbjct: 139 LIKICSDKGQEVLVADAVNLPYREEFGDAAISIAVLHHLSTENRRKKAIEELVRVVKPGG 198

Query: 211 LVLITVWAVEQEDKSLLTKWMPLTEKYVEEWVGPG-----------------------IH 270
            VLITVWA EQED SLLTKW PL+ KYVEEWVGPG                       + 
Sbjct: 199 FVLITVWAAEQEDTSLLTKWTPLSAKYVEEWVGPGSPMNSPRVRNNPFFSLESIPETEVS 258

Query: 271 LRDSK---ENFTG--SMPEN-KSLPSQSGDEL-------------PSCIDENLS--KTAQ 330
            ++ K     F G  S+PE+ +S   Q G+ +                ++E+L   K +Q
Sbjct: 259 TKEQKVENSQFIGLESIPESEESTREQKGESIIPETKASIVEQKDEKSVEESLEALKKSQ 318

Query: 331 QEYFVPWHLPYHRAEVSGTSASALASGLAKKDDKKAAVVYNRYYHVFSEGELERLVSGMD 373
           QEYFVPWHLPYHRAEVSG SASALASGLAKKDD+K AVVYNRYYHVFSEGELERL SG+ 
Sbjct: 319 QEYFVPWHLPYHRAEVSGASASALASGLAKKDDRKGAVVYNRYYHVFSEGELERLASGVG 378

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022152525.14.7e-20893.88alkylated DNA repair protein alkB homolog 8 isoform X1 [Momordica charantia] >XP... [more]
XP_022152527.12.1e-18493.18alkylated DNA repair protein alkB homolog 8 isoform X2 [Momordica charantia][more]
XP_023518340.13.9e-17881.93alkylated DNA repair protein alkB homolog 8-like [Cucurbita pepo subsp. pepo][more]
XP_022939521.15.6e-17782.44alkylated DNA repair protein alkB homolog 8-like isoform X1 [Cucurbita moschata][more]
XP_022939523.15.6e-17782.44alkylated DNA repair protein alkB homolog 8-like isoform X2 [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
Q94A094.7e-12662.18tRNA (carboxymethyluridine(34)-5-O)-methyltransferase OS=Arabidopsis thaliana OX... [more]
Q07G105.8e-6042.11Alkylated DNA repair protein alkB homolog 8 OS=Xenopus tropicalis OX=8364 GN=alk... [more]
Q80Y208.7e-5638.94Alkylated DNA repair protein alkB homolog 8 OS=Mus musculus OX=10090 GN=Alkbh8 P... [more]
A1A4L59.0e-5335.89Alkylated DNA repair protein alkB homolog 8 OS=Bos taurus OX=9913 GN=ALKBH8 PE=2... [more]
Q95K791.0e-5137.33Alkylated DNA repair protein alkB homolog 8 OS=Macaca fascicularis OX=9541 GN=AL... [more]
Match NameE-valueIdentityDescription
A0A6J1DE662.3e-20893.88alkylated DNA repair protein alkB homolog 8 isoform X1 OS=Momordica charantia OX... [more]
A0A6J1DGH61.0e-18493.18alkylated DNA repair protein alkB homolog 8 isoform X2 OS=Momordica charantia OX... [more]
A0A6J1FLV52.7e-17782.44alkylated DNA repair protein alkB homolog 8-like isoform X2 OS=Cucurbita moschat... [more]
A0A6J1FG552.7e-17782.44alkylated DNA repair protein alkB homolog 8-like isoform X1 OS=Cucurbita moschat... [more]
A0A6J1JY215.1e-17681.93alkylated DNA repair protein alkB homolog 8-like isoform X2 OS=Cucurbita maxima ... [more]
Match NameE-valueIdentityDescription
AT1G36310.13.3e-12762.18S-adenosyl-L-methionine-dependent methyltransferases superfamily protein [more]
AT1G36310.23.3e-12762.18S-adenosyl-L-methionine-dependent methyltransferases superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (OHB3-1) v2
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR013216Methyltransferase type 11PFAMPF08241Methyltransf_11coord: 124..214
e-value: 1.1E-14
score: 54.8
NoneNo IPR availableGENE3D3.40.50.150Vaccinia Virus protein VP39coord: 99..372
e-value: 1.2E-59
score: 203.1
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 50..70
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 55..70
NoneNo IPR availablePANTHERPTHR13069:SF33BNAA05G09210D PROTEINcoord: 68..378
NoneNo IPR availablePANTHERPTHR13069UNCHARACTERIZEDcoord: 68..378
NoneNo IPR availableCDDcd02440AdoMet_MTasescoord: 123..219
e-value: 3.9856E-10
score: 54.7434
IPR029063S-adenosyl-L-methionine-dependent methyltransferaseSUPERFAMILY53335S-adenosyl-L-methionine-dependent methyltransferasescoord: 73..241

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Moc04g39510.1Moc04g39510.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0002098 tRNA wobble uridine modification
cellular_component GO:0005737 cytoplasm
cellular_component GO:0005634 nucleus
molecular_function GO:0008168 methyltransferase activity
molecular_function GO:0000049 tRNA binding