Moc04g39340 (gene) Bitter gourd (OHB3-1) v2

Overview
NameMoc04g39340
Typegene
OrganismMomordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
DescriptionDipeptide epimerase
Locationchr4: 29233350 .. 29236221 (+)
RNA-Seq ExpressionMoc04g39340
SyntenyMoc04g39340
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCTTTGTTTCTGTCTGCTGCTCTTCTCCCCTCTTCTTCCGCGCTTCTTCCACGCCTTCCCAGAAGCACTTCCAGGCTCTGCATCGCTTCTAGCCATGGCGGCGGCGTTGAGCTAGTTGCAGATTCTGCTGCCCCTGCCCAAAGGGTAAGTTTCGGGTTCAAAAATTTGGCGGATACTTTTTGGGTGGATGTGCAGAGGGCTGAAGGGAGGCCGTTGAGTGTTGGGCTTAATTCGCCATTGCATATTGGGGAAACGAAGCTTGAAATGGTGGACAATGTTGCAATCAGAGTGGAACTGAGTAATGGGTGTGTGGGTTGGGGGGAAGTTCAAGTGCTACCTCTTGTTACTGATGTCAATCTGGAAACAGCTCTTGGGAAGGCTGAGGATATCTGCAGCTACCTCCGGCGGACCCCGCCGACGACTCTGAATTCGGTGTTTGATGATATTGCCGGACTTCTATCCCCCCGGGAGTTTGCTCCGGTGAGTGAAGTGTTGCTAATTTAGAGCGATTTCATGGAAAGGAATGGAGTAGATGTGTTTATTTAGTAAGTTTGATGGGAATATAATTCACTTGGATCGCATCATTATTCCACAACAGTAGCCTTCTTTTATTTATGAGTTTCAACTGATCCTATAACTTGTTGAGCTAACTCCTTTCACTTACAGCTAACCTGTTGGTCCTACCATATTTGGTTGCTAGATAAAATTCATAGGATTTTAAACCTTAGGTAGGTGACTATCATAGTTTAAATAGTTTAAATGCATTTCCTCGATGCTCTTTTACTTTTTCACTGGTCCCAATACCACTAAGCCAACCAATGGAAGTTCATGTTTCCCCTTTTGATGAAGCAAATCGAGGTGAGGGATTCGAATTTTGACGAGTTCTTTATACCAATGAGTCTTTTGTTATGGGGACAATGACATAGCCCCATATCGTAAGATAAGACAAAGCACATCTCTATTAAAATGAGGTATTTTAGTTCTAAGAAGAAAAACTATACTTGACTTAAATTTAAAGTAGATCATATTATACCATTGCAAGGATATAGAGATAATTTATATTGATGAAATGATTTCAGTTTTGAATATCGGCTCTGGTTACTGTATTAAAAGCAACTCTTTAAGCGGTTTCTGTGTTCTAAAGATCAGGGCTGGGGTGGAGATGGCATTGATTGATGCAGTTGCAAATAGCATCGATGTTCCACTCTGGAGATTATTCGGTGGTGTCACAAGTACCTTAACCACTGCAATTACAGTTGAGCTCAATAAGTTTTTATGCTATAACAGTGTCTTTGCTTGATTCTTGAAGAAGATTTTTCATTATTTGTGATATATGATTCTGCAGGTCCCAATTATTTCCCCAGCAGAGGCGTCACTATTGGCTTCAAAGTATTGCAATCAAGGATTTGAGACTCTTAAGCTTGTTGTTGGGAAAAACTTTGCTGCAGAAATTGCAGCTATTGAAGCCATAAAGGAAGCTCAACCCTGCTGCTCATTCATGTTCGATGCAAACGAAGGATACACGCCCGACGAAGCAATAAAATTTCTCAAGAGATTGAAGGGTAATATTTATTTTTCAAGATTTGAAAATGAGCTAAAACTCTCTGTTATGTAGTATATTTTCAATGATTATGCTCTCTATTCCTTCCCGTCATTGATAAAATGTCTTAAATGATTATTCCATGGCAGATATGGGGATAGTTCCTCTTGTTTTTGAGCAACCTGTAGCCCGAGATGACTGGAAAGGCCTTCGCGAAGTCAGTAACTTAGCTAGAGAGTATGGAGTACCCGTTGCAGCCGATGAAAGCTGTCGGAATTCGACCGATGTTCGAAAGATTATCGACGAAAACCTCGTGGATGCCATAAACATCAAGTTACCCAAGTTTGGAGTCCTTGGAGTTCTAGAAATAATAGAGCTGGAAAGAAAATCAGGCTTGATTCTTATGATTGACAGCATGGCAGAGACGAGACTCGGGACCGGGTTTGCAGGCCATTTGGCTGCTGGCTCTGGCTGCTTCAAGTAACCTTTCACGTCTTTTTTACCACTTTAGGATTCATTCCTGGATTTTCAATGAAGTTTCTTATACAAATTGCAGGTACATTGTTCTTGATGCACCATTTTTATTAGCAGAAGATCCTGTTGTTGGAGGTTATGAAGGTAATCGTTTTTAAATCGCAATAACCGACGTGAACATTCGTGTTTACACACAATAAGCTCAATTTGGTGATGTTGTTGTTATGTTCTCAACCAGCTTCTGGTGCTGTTTACAAGTTCAATAATGCTAGAGGCCAAGGAGGCTTCCTGAATTGGGATCTTCTTCCCGGTGAGATATGTTGCCATGTTTTCATTTCATATCATCTATTTCAGTCTTATTTGCTGCCACTGCATTGTTTGAAGCCATTTTAACAGTGTAGTTGCTACAATCTCCTTCCAAATTAGCCATTCCAAGAGATTAATGTTCTCTCTGATTTTGACCAGGACAAATAATTTGAAAATTTTCTTGAATTCCAATTCAATTTAAGCTTCATTTTCATGTTTCAAACCTGTTCAAGATAAACAACTCTTTCTTGTCCCCAAGGTAAAAAGCAACATCTCATGACGTTTAGGAAATAGTTTTGAAAAGTCATACGTGGCATGTGTTAGGACAACAAATCTCTCTATATTCCACAATAGTATGATATTGTTCATCTTGACCGGAATCTCCCTTGCATGGAGAAGATTGCTTTGCTTGGTCTTAAATTCATGAATTCAGTTGAAAGCTTCCTTTTTCATGTTTTAAATTCTATGATTGAATTCAAAGTCAAAACTTGGATTGGAACCTACTGATTCTGTGGCCTAATTTCTGCAGATGCTGGTGGGTCACCTTAA

mRNA sequence

ATGGCTTTGTTTCTGTCTGCTGCTCTTCTCCCCTCTTCTTCCGCGCTTCTTCCACGCCTTCCCAGAAGCACTTCCAGGCTCTGCATCGCTTCTAGCCATGGCGGCGGCGTTGAGCTAGTTGCAGATTCTGCTGCCCCTGCCCAAAGGGTAAGTTTCGGGTTCAAAAATTTGGCGGATACTTTTTGGGTGGATGTGCAGAGGGCTGAAGGGAGGCCGTTGAGTGTTGGGCTTAATTCGCCATTGCATATTGGGGAAACGAAGCTTGAAATGGTGGACAATGTTGCAATCAGAGTGGAACTGAGTAATGGGTGTGTGGGTTGGGGGGAAGTTCAAGTGCTACCTCTTGTTACTGATGTCAATCTGGAAACAGCTCTTGGGAAGGCTGAGGATATCTGCAGCTACCTCCGGCGGACCCCGCCGACGACTCTGAATTCGGTGTTTGATGATATTGCCGGACTTCTATCCCCCCGGGAGTTTGCTCCGATCAGGGCTGGGGTGGAGATGGCATTGATTGATGCAGTTGCAAATAGCATCGATGTTCCACTCTGGAGATTATTCGGTGGTGTCACAAGTACCTTAACCACTGCAATTACAGTCCCAATTATTTCCCCAGCAGAGGCGTCACTATTGGCTTCAAAGTATTGCAATCAAGGATTTGAGACTCTTAAGCTTGTTGTTGGGAAAAACTTTGCTGCAGAAATTGCAGCTATTGAAGCCATAAAGGAAGCTCAACCCTGCTGCTCATTCATGTTCGATGCAAACGAAGGATACACGCCCGACGAAGCAATAAAATTTCTCAAGAGATTGAAGGATATGGGGATAGTTCCTCTTGTTTTTGAGCAACCTGTAGCCCGAGATGACTGGAAAGGCCTTCGCGAAGTCAGTAACTTAGCTAGAGAGTATGGAGTACCCGTTGCAGCCGATGAAAGCTGTCGGAATTCGACCGATGTTCGAAAGATTATCGACGAAAACCTCGTGGATGCCATAAACATCAAGTTACCCAAGTTTGGAGTCCTTGGAGTTCTAGAAATAATAGAGCTGGAAAGAAAATCAGGCTTGATTCTTATGATTGACAGCATGGCAGAGACGAGACTCGGGACCGGGTTTGCAGGCCATTTGGCTGCTGGCTCTGGCTGCTTCAAGTACATTGTTCTTGATGCACCATTTTTATTAGCAGAAGATCCTGTTGTTGGAGGTTATGAAGCTTCTGGTGCTGTTTACAAGTTCAATAATGCTAGAGGCCAAGGAGGCTTCCTGAATTGGGATCTTCTTCCCGATGCTGGTGGGTCACCTTAA

Coding sequence (CDS)

ATGGCTTTGTTTCTGTCTGCTGCTCTTCTCCCCTCTTCTTCCGCGCTTCTTCCACGCCTTCCCAGAAGCACTTCCAGGCTCTGCATCGCTTCTAGCCATGGCGGCGGCGTTGAGCTAGTTGCAGATTCTGCTGCCCCTGCCCAAAGGGTAAGTTTCGGGTTCAAAAATTTGGCGGATACTTTTTGGGTGGATGTGCAGAGGGCTGAAGGGAGGCCGTTGAGTGTTGGGCTTAATTCGCCATTGCATATTGGGGAAACGAAGCTTGAAATGGTGGACAATGTTGCAATCAGAGTGGAACTGAGTAATGGGTGTGTGGGTTGGGGGGAAGTTCAAGTGCTACCTCTTGTTACTGATGTCAATCTGGAAACAGCTCTTGGGAAGGCTGAGGATATCTGCAGCTACCTCCGGCGGACCCCGCCGACGACTCTGAATTCGGTGTTTGATGATATTGCCGGACTTCTATCCCCCCGGGAGTTTGCTCCGATCAGGGCTGGGGTGGAGATGGCATTGATTGATGCAGTTGCAAATAGCATCGATGTTCCACTCTGGAGATTATTCGGTGGTGTCACAAGTACCTTAACCACTGCAATTACAGTCCCAATTATTTCCCCAGCAGAGGCGTCACTATTGGCTTCAAAGTATTGCAATCAAGGATTTGAGACTCTTAAGCTTGTTGTTGGGAAAAACTTTGCTGCAGAAATTGCAGCTATTGAAGCCATAAAGGAAGCTCAACCCTGCTGCTCATTCATGTTCGATGCAAACGAAGGATACACGCCCGACGAAGCAATAAAATTTCTCAAGAGATTGAAGGATATGGGGATAGTTCCTCTTGTTTTTGAGCAACCTGTAGCCCGAGATGACTGGAAAGGCCTTCGCGAAGTCAGTAACTTAGCTAGAGAGTATGGAGTACCCGTTGCAGCCGATGAAAGCTGTCGGAATTCGACCGATGTTCGAAAGATTATCGACGAAAACCTCGTGGATGCCATAAACATCAAGTTACCCAAGTTTGGAGTCCTTGGAGTTCTAGAAATAATAGAGCTGGAAAGAAAATCAGGCTTGATTCTTATGATTGACAGCATGGCAGAGACGAGACTCGGGACCGGGTTTGCAGGCCATTTGGCTGCTGGCTCTGGCTGCTTCAAGTACATTGTTCTTGATGCACCATTTTTATTAGCAGAAGATCCTGTTGTTGGAGGTTATGAAGCTTCTGGTGCTGTTTACAAGTTCAATAATGCTAGAGGCCAAGGAGGCTTCCTGAATTGGGATCTTCTTCCCGATGCTGGTGGGTCACCTTAA

Protein sequence

MALFLSAALLPSSSALLPRLPRSTSRLCIASSHGGGVELVADSAAPAQRVSFGFKNLADTFWVDVQRAEGRPLSVGLNSPLHIGETKLEMVDNVAIRVELSNGCVGWGEVQVLPLVTDVNLETALGKAEDICSYLRRTPPTTLNSVFDDIAGLLSPREFAPIRAGVEMALIDAVANSIDVPLWRLFGGVTSTLTTAITVPIISPAEASLLASKYCNQGFETLKLVVGKNFAAEIAAIEAIKEAQPCCSFMFDANEGYTPDEAIKFLKRLKDMGIVPLVFEQPVARDDWKGLREVSNLAREYGVPVAADESCRNSTDVRKIIDENLVDAINIKLPKFGVLGVLEIIELERKSGLILMIDSMAETRLGTGFAGHLAAGSGCFKYIVLDAPFLLAEDPVVGGYEASGAVYKFNNARGQGGFLNWDLLPDAGGSP
Homology
BLAST of Moc04g39340 vs. NCBI nr
Match: XP_022133667.1 (L-Ala-D/L-amino acid epimerase-like [Momordica charantia])

HSP 1 Score: 852.8 bits (2202), Expect = 1.3e-243
Identity = 431/431 (100.00%), Postives = 431/431 (100.00%), Query Frame = 0

Query: 1   MALFLSAALLPSSSALLPRLPRSTSRLCIASSHGGGVELVADSAAPAQRVSFGFKNLADT 60
           MALFLSAALLPSSSALLPRLPRSTSRLCIASSHGGGVELVADSAAPAQRVSFGFKNLADT
Sbjct: 59  MALFLSAALLPSSSALLPRLPRSTSRLCIASSHGGGVELVADSAAPAQRVSFGFKNLADT 118

Query: 61  FWVDVQRAEGRPLSVGLNSPLHIGETKLEMVDNVAIRVELSNGCVGWGEVQVLPLVTDVN 120
           FWVDVQRAEGRPLSVGLNSPLHIGETKLEMVDNVAIRVELSNGCVGWGEVQVLPLVTDVN
Sbjct: 119 FWVDVQRAEGRPLSVGLNSPLHIGETKLEMVDNVAIRVELSNGCVGWGEVQVLPLVTDVN 178

Query: 121 LETALGKAEDICSYLRRTPPTTLNSVFDDIAGLLSPREFAPIRAGVEMALIDAVANSIDV 180
           LETALGKAEDICSYLRRTPPTTLNSVFDDIAGLLSPREFAPIRAGVEMALIDAVANSIDV
Sbjct: 179 LETALGKAEDICSYLRRTPPTTLNSVFDDIAGLLSPREFAPIRAGVEMALIDAVANSIDV 238

Query: 181 PLWRLFGGVTSTLTTAITVPIISPAEASLLASKYCNQGFETLKLVVGKNFAAEIAAIEAI 240
           PLWRLFGGVTSTLTTAITVPIISPAEASLLASKYCNQGFETLKLVVGKNFAAEIAAIEAI
Sbjct: 239 PLWRLFGGVTSTLTTAITVPIISPAEASLLASKYCNQGFETLKLVVGKNFAAEIAAIEAI 298

Query: 241 KEAQPCCSFMFDANEGYTPDEAIKFLKRLKDMGIVPLVFEQPVARDDWKGLREVSNLARE 300
           KEAQPCCSFMFDANEGYTPDEAIKFLKRLKDMGIVPLVFEQPVARDDWKGLREVSNLARE
Sbjct: 299 KEAQPCCSFMFDANEGYTPDEAIKFLKRLKDMGIVPLVFEQPVARDDWKGLREVSNLARE 358

Query: 301 YGVPVAADESCRNSTDVRKIIDENLVDAINIKLPKFGVLGVLEIIELERKSGLILMIDSM 360
           YGVPVAADESCRNSTDVRKIIDENLVDAINIKLPKFGVLGVLEIIELERKSGLILMIDSM
Sbjct: 359 YGVPVAADESCRNSTDVRKIIDENLVDAINIKLPKFGVLGVLEIIELERKSGLILMIDSM 418

Query: 361 AETRLGTGFAGHLAAGSGCFKYIVLDAPFLLAEDPVVGGYEASGAVYKFNNARGQGGFLN 420
           AETRLGTGFAGHLAAGSGCFKYIVLDAPFLLAEDPVVGGYEASGAVYKFNNARGQGGFLN
Sbjct: 419 AETRLGTGFAGHLAAGSGCFKYIVLDAPFLLAEDPVVGGYEASGAVYKFNNARGQGGFLN 478

Query: 421 WDLLPDAGGSP 432
           WDLLPDAGGSP
Sbjct: 479 WDLLPDAGGSP 489

BLAST of Moc04g39340 vs. NCBI nr
Match: XP_023550334.1 (L-Ala-D/L-amino acid epimerase-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 727.2 bits (1876), Expect = 8.0e-206
Identity = 367/433 (84.76%), Postives = 393/433 (90.76%), Query Frame = 0

Query: 1   MALFLSAALLPSSSALLPRLPRSTSRLCIASSH-GGGVELVADSAAP-AQRVSFGFKNLA 60
           MAL LSAALLP SS+LL RLPRSTS+L + SSH GGGVEL+ADSAAP A+RVSFGFKNLA
Sbjct: 23  MALVLSAALLPFSSSLLQRLPRSTSKLRVVSSHGGGGVELIADSAAPSAERVSFGFKNLA 82

Query: 61  DTFWVDVQRAEGRPLSVGLNSPLHIGETKLEMVDNVAIRVELSNGCVGWGEVQVLPLVTD 120
           +TFWV+VQRAEGRP SVGLNSPL+ G++KLE V+NVAIRVEL NGCVGWGEVQVLP VTD
Sbjct: 83  ETFWVNVQRAEGRPFSVGLNSPLYFGDSKLETVENVAIRVELKNGCVGWGEVQVLPSVTD 142

Query: 121 VNLETALGKAEDICSYLRRTPPTTLNSVFDDIAGLLSPREFAPIRAGVEMALIDAVANSI 180
           VNLET L KAE +CSYLR TPP TLNS+FDDI GLLSPREFAPIRAGVEMALIDAVANSI
Sbjct: 143 VNLETVLAKAEQVCSYLRHTPPATLNSMFDDITGLLSPREFAPIRAGVEMALIDAVANSI 202

Query: 181 DVPLWRLFGGVTSTLTTAITVPIISPAEASLLASKYCNQGFETLKLVVGKNFAAEIAAIE 240
            VPLWRLFGGVTSTLTTAITVPI+SPAEA+ LASKY NQGF TLKLVVGKNFAAE+AAIE
Sbjct: 203 RVPLWRLFGGVTSTLTTAITVPIVSPAEATFLASKYYNQGFGTLKLVVGKNFAAELAAIE 262

Query: 241 AIKEAQPCCSFMFDANEGYTPDEAIKFLKRLKDMGIVPLVFEQPVARDDWKGLREVSNLA 300
           AI  A PCCS MFDANEGYT +EAIKFL++LKDMGIVPLVFEQPV RDDWKGLR+VSN+A
Sbjct: 263 AIHAALPCCSLMFDANEGYTAEEAIKFLEKLKDMGIVPLVFEQPVDRDDWKGLRQVSNVA 322

Query: 301 REYGVPVAADESCRNSTDVRKIIDENLVDAINIKLPKFGVLGVLEIIELERKSGLILMID 360
           R YG+PVA DESCR+ TDV+KIIDENLVDAINIKLPKFGVLGVLEI  L RKSGL LM+D
Sbjct: 323 RTYGIPVAVDESCRSLTDVQKIIDENLVDAINIKLPKFGVLGVLEITNLARKSGLTLMVD 382

Query: 361 SMAETRLGTGFAGHLAAGSGCFKYIVLDAPFLLAEDPVVGGYEASGAVYKFNNARGQGGF 420
           SMAETRL TGFAGHLAAG GCFKYIVLD PFLLAEDPVVGGYE SGAVYKFNNARGQGGF
Sbjct: 383 SMAETRLATGFAGHLAAGVGCFKYIVLDTPFLLAEDPVVGGYEVSGAVYKFNNARGQGGF 442

Query: 421 LNWDLLPDAGGSP 432
           LNWDLLP+AGG P
Sbjct: 443 LNWDLLPEAGGLP 455

BLAST of Moc04g39340 vs. NCBI nr
Match: XP_038884241.1 (L-Ala-D/L-amino acid epimerase-like [Benincasa hispida])

HSP 1 Score: 723.4 bits (1866), Expect = 1.2e-204
Identity = 365/435 (83.91%), Postives = 394/435 (90.57%), Query Frame = 0

Query: 1   MALFLSAALLP---SSSALLPRLPRSTSRLCIASSHGGGVELVADSAAP-AQRVSFGFKN 60
           MA  LSA L P   SSS+LL  +PR+TS+L I SSHG  VEL+AD AAP AQRVSFGF+N
Sbjct: 12  MASLLSALLFPSSSSSSSLLQHIPRTTSKLQIVSSHGSNVELIADPAAPSAQRVSFGFRN 71

Query: 61  LADTFWVDVQRAEGRPLSVGLNSPLHIGETKLEMVDNVAIRVELSNGCVGWGEVQVLPLV 120
           +ADTFWV+VQRAEGRPLS+GLNSPLH G +KLE V+NVAIRVELSNGCVGWGEVQVLP V
Sbjct: 72  VADTFWVNVQRAEGRPLSIGLNSPLHFGNSKLETVENVAIRVELSNGCVGWGEVQVLPFV 131

Query: 121 TDVNLETALGKAEDICSYLRRTPPTTLNSVFDDIAGLLSPREFAPIRAGVEMALIDAVAN 180
           TDV+LE AL KAE++C+YLR TPP TL SVFDDI G+LSPREFAPIRAGVEMALIDAVAN
Sbjct: 132 TDVSLEMALAKAEEVCNYLRLTPPATLTSVFDDITGILSPREFAPIRAGVEMALIDAVAN 191

Query: 181 SIDVPLWRLFGGVTSTLTTAITVPIISPAEASLLASKYCNQGFETLKLVVGKNFAAEIAA 240
           SI+VPLWRLFGGVTSTLTTAITVPIISP EAS+LASKY NQGF+TLKLVVGKNFAAEIAA
Sbjct: 192 SINVPLWRLFGGVTSTLTTAITVPIISPEEASILASKYYNQGFKTLKLVVGKNFAAEIAA 251

Query: 241 IEAIKEAQPCCSFMFDANEGYTPDEAIKFLKRLKDMGIVPLVFEQPVARDDWKGLREVSN 300
           IEAI  AQPCCSFMFDANEGYT DEAIKFL++LKDMG+VPLVFEQPV RDDWKGL EVSN
Sbjct: 252 IEAIHAAQPCCSFMFDANEGYTTDEAIKFLEKLKDMGVVPLVFEQPVDRDDWKGLHEVSN 311

Query: 301 LAREYGVPVAADESCRNSTDVRKIIDENLVDAINIKLPKFGVLGVLEIIELERKSGLILM 360
           +AR YG+PVA DESCR+ TDV+KIIDENLVDAINIKLPKFGVLGVLEII+L RKSGLILM
Sbjct: 312 VARMYGIPVAVDESCRSLTDVQKIIDENLVDAINIKLPKFGVLGVLEIIKLARKSGLILM 371

Query: 361 IDSMAETRLGTGFAGHLAAGSGCFKYIVLDAPFLLAEDPVVGGYEASGAVYKFNNARGQG 420
           +DSMAETRL TGFAGHLAAG GCFKYIVLD PFLLAED VVGGYEASGAVYKFNNARGQG
Sbjct: 372 VDSMAETRLATGFAGHLAAGVGCFKYIVLDTPFLLAEDHVVGGYEASGAVYKFNNARGQG 431

Query: 421 GFLNWDLLPDAGGSP 432
           GFLNW+LLPDAGG P
Sbjct: 432 GFLNWNLLPDAGGLP 446

BLAST of Moc04g39340 vs. NCBI nr
Match: KAG6578347.1 (L-Ala-D/L-amino acid epimerase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 718.8 bits (1854), Expect = 2.9e-203
Identity = 365/433 (84.30%), Postives = 392/433 (90.53%), Query Frame = 0

Query: 1   MALFLSAALLPSSSALLPRLPRSTSRLCIASSH-GGGVELVADSAA-PAQRVSFGFKNLA 60
           MAL LSAALLP SS+LL RLPRSTS+L + SSH GGGVEL+ADSAA PA+RVSFGFKNLA
Sbjct: 23  MALVLSAALLPFSSSLLQRLPRSTSKLRVVSSHGGGGVELIADSAAPPAERVSFGFKNLA 82

Query: 61  DTFWVDVQRAEGRPLSVGLNSPLHIGETKLEMVDNVAIRVELSNGCVGWGEVQVLPLVTD 120
           +TFWV+VQRAEGRP SVGLNSPL+ G++KLE V+NVAIRVEL NGCVGWGEVQV   VTD
Sbjct: 83  ETFWVNVQRAEGRPFSVGLNSPLYFGDSKLETVENVAIRVELKNGCVGWGEVQV--SVTD 142

Query: 121 VNLETALGKAEDICSYLRRTPPTTLNSVFDDIAGLLSPREFAPIRAGVEMALIDAVANSI 180
           VNLET L KAE +CSYLR TPP TLNS+FDDI GLLSPREFAPIRAGVEMALIDAVANSI
Sbjct: 143 VNLETVLAKAEQVCSYLRHTPPATLNSMFDDITGLLSPREFAPIRAGVEMALIDAVANSI 202

Query: 181 DVPLWRLFGGVTSTLTTAITVPIISPAEASLLASKYCNQGFETLKLVVGKNFAAEIAAIE 240
            VPLWRLFGGVTSTLTTAITVPI+SPAEA++LASKY NQGF TLKLVVGKNFAAE+AAIE
Sbjct: 203 RVPLWRLFGGVTSTLTTAITVPIVSPAEATILASKYYNQGFGTLKLVVGKNFAAELAAIE 262

Query: 241 AIKEAQPCCSFMFDANEGYTPDEAIKFLKRLKDMGIVPLVFEQPVARDDWKGLREVSNLA 300
           AI  A PCCS MFDANEGYT +EAIKFL++LKDMGIVPLVFEQPV RDDWKGLR+VSN+A
Sbjct: 263 AIHAALPCCSLMFDANEGYTAEEAIKFLEKLKDMGIVPLVFEQPVDRDDWKGLRQVSNVA 322

Query: 301 REYGVPVAADESCRNSTDVRKIIDENLVDAINIKLPKFGVLGVLEIIELERKSGLILMID 360
           R YG+PVA DESCR+ TDV+KIIDENLVDAINIKLPKFGVLGVLEI  L RKSGL LM+D
Sbjct: 323 RTYGIPVAVDESCRSLTDVQKIIDENLVDAINIKLPKFGVLGVLEITNLARKSGLTLMVD 382

Query: 361 SMAETRLGTGFAGHLAAGSGCFKYIVLDAPFLLAEDPVVGGYEASGAVYKFNNARGQGGF 420
           SMAETRL TGFAGHLAAG GCFKYIVLD PFLLAEDPVVGGYE SGAVYKFNNARGQGGF
Sbjct: 383 SMAETRLATGFAGHLAAGVGCFKYIVLDTPFLLAEDPVVGGYEVSGAVYKFNNARGQGGF 442

Query: 421 LNWDLLPDAGGSP 432
           LNWDLLP+AGG P
Sbjct: 443 LNWDLLPEAGGLP 453

BLAST of Moc04g39340 vs. NCBI nr
Match: XP_022938632.1 (L-Ala-D/L-amino acid epimerase-like [Cucurbita moschata])

HSP 1 Score: 716.8 bits (1849), Expect = 1.1e-202
Identity = 364/431 (84.45%), Postives = 391/431 (90.72%), Query Frame = 0

Query: 1   MALFLSAALLPSSSALLPRLPRSTSRLCIASSH-GGGVELVADSAAP-AQRVSFGFKNLA 60
           MAL LSAALLP SS+LL RLPRSTS+L + SSH GGGVEL+ADSAAP A+RVSFGFKNLA
Sbjct: 1   MALVLSAALLPFSSSLLQRLPRSTSKLRVVSSHGGGGVELIADSAAPSAERVSFGFKNLA 60

Query: 61  DTFWVDVQRAEGRPLSVGLNSPLHIGETKLEMVDNVAIRVELSNGCVGWGEVQVLPLVTD 120
           +TFWV+VQRAEGRP SVGLNSPL+ G++KLE V+NVAIRVEL NGCVGWGEVQV   VTD
Sbjct: 61  ETFWVNVQRAEGRPFSVGLNSPLYFGDSKLETVENVAIRVELKNGCVGWGEVQV--SVTD 120

Query: 121 VNLETALGKAEDICSYLRRTPPTTLNSVFDDIAGLLSPREFAPIRAGVEMALIDAVANSI 180
           VNLET L KAE +CSYLR TPP TLNS+FDDI GLLSPREFAPIRAGVEMALIDAVANSI
Sbjct: 121 VNLETVLAKAEQVCSYLRHTPPATLNSMFDDITGLLSPREFAPIRAGVEMALIDAVANSI 180

Query: 181 DVPLWRLFGGVTSTLTTAITVPIISPAEASLLASKYCNQGFETLKLVVGKNFAAEIAAIE 240
            VPLWRLFGGVTSTLTTAITVPI+SPAEA++LASKY NQGF TLKLVVGKNFAAE+AAIE
Sbjct: 181 RVPLWRLFGGVTSTLTTAITVPIVSPAEATILASKYYNQGFGTLKLVVGKNFAAELAAIE 240

Query: 241 AIKEAQPCCSFMFDANEGYTPDEAIKFLKRLKDMGIVPLVFEQPVARDDWKGLREVSNLA 300
           AI  A PCCS MFDANEGYT +EAIKFL++LKDMGIVPLVFEQPV RDDWKGLR+VSN+A
Sbjct: 241 AIHAALPCCSLMFDANEGYTAEEAIKFLEKLKDMGIVPLVFEQPVDRDDWKGLRQVSNVA 300

Query: 301 REYGVPVAADESCRNSTDVRKIIDENLVDAINIKLPKFGVLGVLEIIELERKSGLILMID 360
           R YG+PVA DESCR+ TDV+KIIDENLVDAINIKLPKFGVLGVLEI  L RKSGL LM+D
Sbjct: 301 RTYGIPVAVDESCRSLTDVQKIIDENLVDAINIKLPKFGVLGVLEITNLARKSGLTLMVD 360

Query: 361 SMAETRLGTGFAGHLAAGSGCFKYIVLDAPFLLAEDPVVGGYEASGAVYKFNNARGQGGF 420
           SMAETRL TGFAGHLAAG GCFKYIVLD PFLLAEDPVVGGYE SGAVYKFNNARGQGGF
Sbjct: 361 SMAETRLATGFAGHLAAGVGCFKYIVLDTPFLLAEDPVVGGYEVSGAVYKFNNARGQGGF 420

Query: 421 LNWDLLPDAGG 430
           LNWDLLP+AGG
Sbjct: 421 LNWDLLPEAGG 429

BLAST of Moc04g39340 vs. ExPASy Swiss-Prot
Match: B9I2J6 (L-Ala-D/L-amino acid epimerase OS=Populus trichocarpa OX=3694 GN=POPTR_0012s05040g PE=3 SV=2)

HSP 1 Score: 439.5 bits (1129), Expect = 4.4e-122
Identity = 219/378 (57.94%), Postives = 278/378 (73.54%), Query Frame = 0

Query: 46  PAQRVSFGFKNLADTFWVDVQRAEGRPLSVGLNSPLHIGETKLEMVDNVAIRVELSNGCV 105
           P Q  +F F++L +TF VDV+RAE RPL+V L +P  I  ++L+ V+NVAIR+ELS+GCV
Sbjct: 41  PKQVSTFEFRDLMETFAVDVKRAENRPLNVPLIAPFTIASSRLDKVENVAIRIELSDGCV 100

Query: 106 GWGEVQVLPLVTDVNLETALGKAEDICSYLRRTPPTTLNSVFDDIAGLLSPREFAPIRAG 165
           GWGE  +LP VT  +  TA+ KA + C  L+ +    L  V + ++ +L   EFA +RAG
Sbjct: 101 GWGEAPILPFVTAEDQSTAMIKAREACELLKNSSSMKLGLVLERVSEILPGHEFASVRAG 160

Query: 166 VEMALIDAVANSIDVPLWRLFGGVTSTLTTAITVPIISPAEASLLASKYCNQGFETLKLV 225
           VEMALIDAVA SI+VPLW LFGG + ++TT IT+PI+S AEA+ LASKY  QGF+TLKL 
Sbjct: 161 VEMALIDAVAKSINVPLWILFGGASDSITTDITIPIVSSAEAAELASKYRKQGFQTLKLK 220

Query: 226 VGKNFAAEIAAIEAIKEAQPCCSFMFDANEGYTPDEAIKFLKRLKDMGIVPLVFEQPVAR 285
           VGKN   +I  ++AI+   P C F+ DANEGY P+EAI+ L+ L  MG+ P++FEQPV R
Sbjct: 221 VGKNLKEDIEVLQAIRAVHPDCLFILDANEGYKPEEAIEVLEELHKMGVTPILFEQPVHR 280

Query: 286 DDWKGLREVSNLAR-EYGVPVAADESCRNSTDVRKIIDENLVDAINIKLPKFGVLGVLEI 345
           DDW+GL  V+++A+ +YGV VAADESCR+  D ++II  NL D INIKL K GV+G LEI
Sbjct: 281 DDWEGLGHVTHIAKGKYGVSVAADESCRSLVDAKRIIKGNLADVINIKLAKVGVVGGLEI 340

Query: 346 IELERKSGLILMIDSMAETRLGTGFAGHLAAGSGCFKYIVLDAPFLLAEDPVVGGYEASG 405
           IE  R SGL LMI  M ETRL  GFAGHLAAG GCFK+I LD P LL+EDPV+ GYE SG
Sbjct: 341 IEEARTSGLDLMIGGMVETRLAMGFAGHLAAGFGCFKFIDLDTPLLLSEDPVLEGYEVSG 400

Query: 406 AVYKFNNARGQGGFLNWD 423
           AVYKF +A+G  GFL+WD
Sbjct: 401 AVYKFTDAQGHAGFLDWD 418

BLAST of Moc04g39340 vs. ExPASy Swiss-Prot
Match: A9B055 (Aromatic dipeptide epimerase OS=Herpetosiphon aurantiacus (strain ATCC 23779 / DSM 785 / 114-95) OX=316274 GN=Haur_1114 PE=1 SV=1)

HSP 1 Score: 186.0 bits (471), Expect = 8.7e-46
Identity = 114/355 (32.11%), Postives = 181/355 (50.99%), Query Frame = 0

Query: 65  VQRAEGRPLSVGLNSPLHIGETKLEMVDNVAIRVELSNGCVGWGEVQVLPLVTDVNLETA 124
           +Q      +++ L  P  I      +  NV ++V+L++G +G GE    P V+       
Sbjct: 5   IQAISAEAINLPLTEPFAIASGAQAVAANVLVKVQLADGTLGLGEAAPFPAVSGETQTGT 64

Query: 125 LGKAEDICSYLRRTPPTTLNSVFDDIAGLL--SPREFAPIRAGVEMALIDAVANSIDVPL 184
               E + S+L           +  +A +L  +  E A  R G+EMA++DA+     +PL
Sbjct: 65  SAAIERLQSHLLGADVRG----WRKLAAMLDHAEHEAAAARCGLEMAMLDALTRHYHMPL 124

Query: 185 WRLFGGVTSTLTTAITVPIISPAEASLLASKYCNQGFETLKL-VVGKNFAAEIAAIEAIK 244
              FGGV+  L T +T+       A+  A     +G +++K+   G + A ++A + AI 
Sbjct: 125 HVFFGGVSKQLETDMTITAGDEVHAAASAKAILARGIKSIKVKTAGVDVAYDLARLRAIH 184

Query: 245 EAQPCCSFMFDANEGYTPDEAIKFLKRLKDMGIVPLVFEQPVARDDWKGLREVSNLAREY 304
           +A P    + D N GY  + A+ F    K   I  ++FEQP+ R+DW G+ +V+    + 
Sbjct: 185 QAAPTAPLIVDGNCGYDVERALAFCAACKAESIPMVLFEQPLPREDWAGMAQVT---AQS 244

Query: 305 GVPVAADESCRNSTDVRKIIDENLVDAINIKLPKFGVLGVLEIIELERKSGLILMIDSMA 364
           G  VAADES R++ DV +I  E     INIKL K GV   L++I + + +GL LMI  M 
Sbjct: 245 GFAVAADESARSAHDVLRIAREGTASVINIKLMKAGVAEGLKMIAIAQAAGLGLMIGGMV 304

Query: 365 ETRLGTGFAGHLAAGSGCFKYIVLDAPFLLAEDPVVGGYEASGAVYKFNNARGQG 417
           E+ L   F+ +LAAG+G F +I LD P  +AE P +GG+  +G   +  +  G G
Sbjct: 305 ESILAMSFSANLAAGNGGFDFIDLDTPLFIAEHPFIGGFAQTGGTLQLADVAGHG 352

BLAST of Moc04g39340 vs. ExPASy Swiss-Prot
Match: B5EFW2 (Hydrophobic dipeptide epimerase OS=Geobacter bemidjiensis (strain Bem / ATCC BAA-1014 / DSM 16622) OX=404380 GN=Gbem_2417 PE=3 SV=1)

HSP 1 Score: 170.2 bits (430), Expect = 5.0e-41
Identity = 103/332 (31.02%), Postives = 161/332 (48.49%), Query Frame = 0

Query: 65  VQRAEGRPLSVGLNSPLHIGETKLEMVDNVAIRVELSNGCVGWGEVQVLPLVTDVNLETA 124
           +Q A    +   L SP  I   + + ++NV +++   +G  G+GE  V   +T   +   
Sbjct: 7   IQDAVASVIRAPLASPFRIATGQHDELENVFLKLTTRDGVSGYGEAAVASHITGETVPGT 66

Query: 125 LGKAEDICSYLRRTPPTTLNSVFDDIAGLLSPREFAPIRAGVEMALIDAVANSIDVPLWR 184
           L   ++  + LR        S     A   +        A +EMAL+D  +    +P +R
Sbjct: 67  LANLQNAAAALRGQTVDDAESACRQFAAAFAGNHAG--LAALEMALLDLSSRVRGIPFYR 126

Query: 185 LFGGVTS-----TLTTAITVPIISPAEASLLASKYCNQGFETLKLVVGKNFAAEIAAIEA 244
           LF  V +       +T ITV I S  EA   A ++ ++GF+  K+ +G++   ++A + A
Sbjct: 127 LFAPVAALEPRLAFSTDITVVIGSLDEARATAREFASRGFKAFKIKIGRDEQLDLARVLA 186

Query: 245 IKEAQPCCSFMFDANEGYTPDEAIKFLKRLKDMGIVPLVFEQPVARDDWKGLREVSNLAR 304
           + E  P    + DAN G++    + FL RL   G+ P++ EQPV + DW GL E++    
Sbjct: 187 VHEIAPDSQIILDANMGFSAGSMLAFLDRLAAKGVRPVLLEQPVPKMDWDGLSEITAALT 246

Query: 305 EYGVPVAADESCRNSTDVRKIIDENLVDAINIKLPKFGVLGVLEIIELERKSGLILMIDS 364
                V ADES  +  D R+ ID N V AIN+K  K G+L   EI  L    G+ LM+ +
Sbjct: 247 GSETLVCADESVGSLADARRAIDSNAVSAINVKFMKSGILEGAEIARLAASRGIRLMLGA 306

Query: 365 MAETRLGTGFAGHLAAGSGCFKYIVLDAPFLL 392
           M E+ L    + H AAG  CF Y+ +D  F L
Sbjct: 307 MMESALAVTASAHFAAGLACFDYLDMDTTFFL 336

BLAST of Moc04g39340 vs. ExPASy Swiss-Prot
Match: O34508 (L-Ala-D/L-Glu epimerase OS=Bacillus subtilis (strain 168) OX=224308 GN=ykfB PE=1 SV=1)

HSP 1 Score: 169.1 bits (427), Expect = 1.1e-40
Identity = 113/354 (31.92%), Postives = 170/354 (48.02%), Query Frame = 0

Query: 67  RAEGRPLSVGLNSPLHIGETKLEMVDNVAIRVELSNGCVGWGEVQVLPLVTDVNLETALG 126
           R E   ++V L  P       +   ++V +R+   +G VGWGE     ++T  ++++   
Sbjct: 5   RIETSRIAVPLTKPFKTALRTVYTAESVIVRITYDSGAVGWGEAPPTLVITGDSMDSIES 64

Query: 127 KAEDICS-YLRRTPPTTLNSVFDDIAGLLSPREFAPIRAGVEMALIDAVANSIDVPLWRL 186
               +    L         ++  DI  LL+    A  +A VEMAL D  A    +PL+++
Sbjct: 65  AIHHVLKPALLGKSLAGYEAILHDIQHLLTGNMSA--KAAVEMALYDGWAQMCGLPLYQM 124

Query: 187 FGGVTSTLTTAITVPIISPAEASLLASKYCNQGFETLKLVVGK-NFAAEIAAIEAI-KEA 246
            GG   TL T  TV + SP E +  A  Y  QGF+TLK+ VGK + A +IA I+ I K  
Sbjct: 125 LGGYRDTLETDYTVSVNSPEEMAADAENYLKQGFQTLKIKVGKDDIATDIARIQEIRKRV 184

Query: 247 QPCCSFMFDANEGYTPDEAIKFLKRLKDMGIVPLVFEQPVARDDWKGLREVSNLAREYGV 306
                   DAN+G+ P EA+  +++++D G+   + EQPV +DD  GL++V++       
Sbjct: 185 GSAVKLRLDANQGWRPKEAVTAIRKMEDAGLGIELVEQPVHKDDLAGLKKVTDAT---DT 244

Query: 307 PVAADESCRNSTDVRKIIDENLVDAINIKLPKF-GVLGVLEIIELERKSGLILMIDSMAE 366
           P+ ADES        +++     D INIKL K  G+ G  +I  +    G+  M+ SM E
Sbjct: 245 PIMADESVFTPRQAFEVLQTRSADLINIKLMKAGGISGAEKINAMAEACGVECMVGSMIE 304

Query: 367 TRLGTGFAGHLAAGSGCFKYIVLDAPFLLAEDPVVGGYEASGAVYKFNNARGQG 417
           T+LG   A H AA          DAP +L  D   GG   SG+        G G
Sbjct: 305 TKLGITAAAHFAASKRNITRFDFDAPLMLKTDVFNGGITYSGSTISMPGKPGLG 353

BLAST of Moc04g39340 vs. ExPASy Swiss-Prot
Match: Q9WXM1 (L-Ala-D/L-Glu epimerase OS=Thermotoga maritima (strain ATCC 43589 / DSM 3109 / JCM 10099 / NBRC 100826 / MSB8) OX=243274 GN=TM_0006 PE=1 SV=1)

HSP 1 Score: 162.5 bits (410), Expect = 1.0e-38
Identity = 104/325 (32.00%), Postives = 157/325 (48.31%), Query Frame = 0

Query: 80  PLHIGETKLEMVDNVAIRVELSNGCVGWGEVQVLPLVTDVNLETALGKAEDICSYLRRTP 139
           P HI  +      NV + + L +G  G+GE      V    +E  L     +   +    
Sbjct: 19  PFHITGSVSSESRNVEVEIVLESGVKGYGEASPSFRVNGERVEALLAIENAVREMITGID 78

Query: 140 PTTLNSVFDDIAGLLSPREFAPIRAGVEMALIDAVANSIDVPLWRLFGGVTSTLTTAITV 199
                 +F+    L     F  ++A V+ A +DA++  +   +  L GG    + T  TV
Sbjct: 79  VRNYARIFEITDRLFG---FPSLKAAVQFATLDALSQELGTQVCYLLGGKRDEIETDKTV 138

Query: 200 PIISPAEASLLASKYCNQGFETLKLVVGKNFAAEIAAIEAIKEAQPCCSFMFDANEGYTP 259
            I +       A K   +GF  +K+ VG+N   +I A+E I +      ++ DAN GYT 
Sbjct: 139 GIDTVENRVKEAKKIFEEGFRVIKIKVGENLKEDIEAVEEIAKVTRGAKYIVDANMGYTQ 198

Query: 260 DEAIKFLKRLKDMGIVPLVFEQPVARDDWKGLREVSNLAREYGVPVAADESCRNSTDVRK 319
            EA++F + +   GI   V+EQPV R+D +GL+ V         PVAADES R   DV +
Sbjct: 199 KEAVEFARAVYQKGIDIAVYEQPVRREDIEGLKFV---RFHSPFPVAADESARTKFDVMR 258

Query: 320 IIDENLVDAINIKLPKFGVLGVLEIIELERKSGLILMIDSMAETRLGTGFAGHLAAGSGC 379
           ++ E  VD +NIKL K G+   L I+E+   SGL LMI  M E+ LG   + H A G+G 
Sbjct: 259 LVKEEAVDYVNIKLMKSGISDALAIVEIAESSGLKLMIGCMGESSLGINQSVHFALGTGA 318

Query: 380 FKYIVLDAPFLLAEDPVVGGYEASG 405
           F++  LD+  +L E+   G +   G
Sbjct: 319 FEFHDLDSHLMLKEEVFRGKFIQDG 337

BLAST of Moc04g39340 vs. ExPASy TrEMBL
Match: A0A6J1BXC6 (L-Ala-D/L-amino acid epimerase-like OS=Momordica charantia OX=3673 GN=LOC111006196 PE=4 SV=1)

HSP 1 Score: 852.8 bits (2202), Expect = 6.1e-244
Identity = 431/431 (100.00%), Postives = 431/431 (100.00%), Query Frame = 0

Query: 1   MALFLSAALLPSSSALLPRLPRSTSRLCIASSHGGGVELVADSAAPAQRVSFGFKNLADT 60
           MALFLSAALLPSSSALLPRLPRSTSRLCIASSHGGGVELVADSAAPAQRVSFGFKNLADT
Sbjct: 59  MALFLSAALLPSSSALLPRLPRSTSRLCIASSHGGGVELVADSAAPAQRVSFGFKNLADT 118

Query: 61  FWVDVQRAEGRPLSVGLNSPLHIGETKLEMVDNVAIRVELSNGCVGWGEVQVLPLVTDVN 120
           FWVDVQRAEGRPLSVGLNSPLHIGETKLEMVDNVAIRVELSNGCVGWGEVQVLPLVTDVN
Sbjct: 119 FWVDVQRAEGRPLSVGLNSPLHIGETKLEMVDNVAIRVELSNGCVGWGEVQVLPLVTDVN 178

Query: 121 LETALGKAEDICSYLRRTPPTTLNSVFDDIAGLLSPREFAPIRAGVEMALIDAVANSIDV 180
           LETALGKAEDICSYLRRTPPTTLNSVFDDIAGLLSPREFAPIRAGVEMALIDAVANSIDV
Sbjct: 179 LETALGKAEDICSYLRRTPPTTLNSVFDDIAGLLSPREFAPIRAGVEMALIDAVANSIDV 238

Query: 181 PLWRLFGGVTSTLTTAITVPIISPAEASLLASKYCNQGFETLKLVVGKNFAAEIAAIEAI 240
           PLWRLFGGVTSTLTTAITVPIISPAEASLLASKYCNQGFETLKLVVGKNFAAEIAAIEAI
Sbjct: 239 PLWRLFGGVTSTLTTAITVPIISPAEASLLASKYCNQGFETLKLVVGKNFAAEIAAIEAI 298

Query: 241 KEAQPCCSFMFDANEGYTPDEAIKFLKRLKDMGIVPLVFEQPVARDDWKGLREVSNLARE 300
           KEAQPCCSFMFDANEGYTPDEAIKFLKRLKDMGIVPLVFEQPVARDDWKGLREVSNLARE
Sbjct: 299 KEAQPCCSFMFDANEGYTPDEAIKFLKRLKDMGIVPLVFEQPVARDDWKGLREVSNLARE 358

Query: 301 YGVPVAADESCRNSTDVRKIIDENLVDAINIKLPKFGVLGVLEIIELERKSGLILMIDSM 360
           YGVPVAADESCRNSTDVRKIIDENLVDAINIKLPKFGVLGVLEIIELERKSGLILMIDSM
Sbjct: 359 YGVPVAADESCRNSTDVRKIIDENLVDAINIKLPKFGVLGVLEIIELERKSGLILMIDSM 418

Query: 361 AETRLGTGFAGHLAAGSGCFKYIVLDAPFLLAEDPVVGGYEASGAVYKFNNARGQGGFLN 420
           AETRLGTGFAGHLAAGSGCFKYIVLDAPFLLAEDPVVGGYEASGAVYKFNNARGQGGFLN
Sbjct: 419 AETRLGTGFAGHLAAGSGCFKYIVLDAPFLLAEDPVVGGYEASGAVYKFNNARGQGGFLN 478

Query: 421 WDLLPDAGGSP 432
           WDLLPDAGGSP
Sbjct: 479 WDLLPDAGGSP 489

BLAST of Moc04g39340 vs. ExPASy TrEMBL
Match: A0A6J1FEM1 (L-Ala-D/L-amino acid epimerase-like OS=Cucurbita moschata OX=3662 GN=LOC111444808 PE=4 SV=1)

HSP 1 Score: 716.8 bits (1849), Expect = 5.2e-203
Identity = 364/431 (84.45%), Postives = 391/431 (90.72%), Query Frame = 0

Query: 1   MALFLSAALLPSSSALLPRLPRSTSRLCIASSH-GGGVELVADSAAP-AQRVSFGFKNLA 60
           MAL LSAALLP SS+LL RLPRSTS+L + SSH GGGVEL+ADSAAP A+RVSFGFKNLA
Sbjct: 1   MALVLSAALLPFSSSLLQRLPRSTSKLRVVSSHGGGGVELIADSAAPSAERVSFGFKNLA 60

Query: 61  DTFWVDVQRAEGRPLSVGLNSPLHIGETKLEMVDNVAIRVELSNGCVGWGEVQVLPLVTD 120
           +TFWV+VQRAEGRP SVGLNSPL+ G++KLE V+NVAIRVEL NGCVGWGEVQV   VTD
Sbjct: 61  ETFWVNVQRAEGRPFSVGLNSPLYFGDSKLETVENVAIRVELKNGCVGWGEVQV--SVTD 120

Query: 121 VNLETALGKAEDICSYLRRTPPTTLNSVFDDIAGLLSPREFAPIRAGVEMALIDAVANSI 180
           VNLET L KAE +CSYLR TPP TLNS+FDDI GLLSPREFAPIRAGVEMALIDAVANSI
Sbjct: 121 VNLETVLAKAEQVCSYLRHTPPATLNSMFDDITGLLSPREFAPIRAGVEMALIDAVANSI 180

Query: 181 DVPLWRLFGGVTSTLTTAITVPIISPAEASLLASKYCNQGFETLKLVVGKNFAAEIAAIE 240
            VPLWRLFGGVTSTLTTAITVPI+SPAEA++LASKY NQGF TLKLVVGKNFAAE+AAIE
Sbjct: 181 RVPLWRLFGGVTSTLTTAITVPIVSPAEATILASKYYNQGFGTLKLVVGKNFAAELAAIE 240

Query: 241 AIKEAQPCCSFMFDANEGYTPDEAIKFLKRLKDMGIVPLVFEQPVARDDWKGLREVSNLA 300
           AI  A PCCS MFDANEGYT +EAIKFL++LKDMGIVPLVFEQPV RDDWKGLR+VSN+A
Sbjct: 241 AIHAALPCCSLMFDANEGYTAEEAIKFLEKLKDMGIVPLVFEQPVDRDDWKGLRQVSNVA 300

Query: 301 REYGVPVAADESCRNSTDVRKIIDENLVDAINIKLPKFGVLGVLEIIELERKSGLILMID 360
           R YG+PVA DESCR+ TDV+KIIDENLVDAINIKLPKFGVLGVLEI  L RKSGL LM+D
Sbjct: 301 RTYGIPVAVDESCRSLTDVQKIIDENLVDAINIKLPKFGVLGVLEITNLARKSGLTLMVD 360

Query: 361 SMAETRLGTGFAGHLAAGSGCFKYIVLDAPFLLAEDPVVGGYEASGAVYKFNNARGQGGF 420
           SMAETRL TGFAGHLAAG GCFKYIVLD PFLLAEDPVVGGYE SGAVYKFNNARGQGGF
Sbjct: 361 SMAETRLATGFAGHLAAGVGCFKYIVLDTPFLLAEDPVVGGYEVSGAVYKFNNARGQGGF 420

Query: 421 LNWDLLPDAGG 430
           LNWDLLP+AGG
Sbjct: 421 LNWDLLPEAGG 429

BLAST of Moc04g39340 vs. ExPASy TrEMBL
Match: A0A1S3B326 (L-Ala-D/L-amino acid epimerase-like OS=Cucumis melo OX=3656 GN=LOC103485448 PE=4 SV=1)

HSP 1 Score: 698.7 bits (1802), Expect = 1.5e-197
Identity = 352/430 (81.86%), Postives = 388/430 (90.23%), Query Frame = 0

Query: 5   LSAALLPSSS-ALLPRLPRST-SRLCIASSHGGGVELVADSAAP-AQRVSFGFKNLADTF 64
           LSAALLPSSS  L  R+PR+T S+L I S H   VEL+AD   P +QR+SFGF+N+ADTF
Sbjct: 6   LSAALLPSSSFGLHLRIPRTTSSKLRILSIHATNVELIADPPTPSSQRLSFGFQNVADTF 65

Query: 65  WVDVQRAEGRPLSVGLNSPLHIGETKLEMVDNVAIRVELSNGCVGWGEVQVLPLVTDVNL 124
           WV+VQRAEGRPLS+GLNSPLH G +KLE +DNVA+RVELSNGCVGWGEVQVLP VTDV+L
Sbjct: 66  WVNVQRAEGRPLSIGLNSPLHFGNSKLETLDNVAMRVELSNGCVGWGEVQVLPSVTDVSL 125

Query: 125 ETALGKAEDICSYLRRTPPTTLNSVFDDIAGLLSPREFAPIRAGVEMALIDAVANSIDVP 184
           E AL KA+++C++LRRTPP TL+SVF+D+ GLLSPREFAPIRAGVEMALIDAVANSI+VP
Sbjct: 126 EMALAKAQEVCNFLRRTPPATLSSVFNDVTGLLSPREFAPIRAGVEMALIDAVANSINVP 185

Query: 185 LWRLFGGVTSTLTTAITVPIISPAEASLLASKYCNQGFETLKLVVGKNFAAEIAAIEAIK 244
           LWRLFGGVTSTLTT ITVPI+SP EAS+LASK+CNQGFETLKLVVGKNFAAEIAAIEAI 
Sbjct: 186 LWRLFGGVTSTLTTGITVPILSPEEASILASKFCNQGFETLKLVVGKNFAAEIAAIEAIH 245

Query: 245 EAQPCCSFMFDANEGYTPDEAIKFLKRLKDMGIVPLVFEQPVARDDWKGLREVSNLAREY 304
            AQPCCSFMFDANEGYTPDEAIKFL++LKD+GIVPLVFEQPV RDDWKGL+EVSN+AR +
Sbjct: 246 AAQPCCSFMFDANEGYTPDEAIKFLEKLKDVGIVPLVFEQPVDRDDWKGLQEVSNVARMH 305

Query: 305 GVPVAADESCRNSTDVRKIIDENLVDAINIKLPKFGVLGVLEIIELERKSGLILMIDSMA 364
           G+PVA DESCR+ TDV KIIDENLVDAINIKLPKFGVLG LEII L RKSGLILM+DSMA
Sbjct: 306 GIPVAVDESCRSLTDVCKIIDENLVDAINIKLPKFGVLGALEIINLARKSGLILMVDSMA 365

Query: 365 ETRLGTGFAGHLAAGSGCFKYIVLDAPFLLAEDPVVGGYEASGAVYKFNNARGQGGFLNW 424
           ETRLGTGFAGHLAAG GCFKYIVLD P LLAEDPVVGGYEASGAVYKFNNARGQGGFLNW
Sbjct: 366 ETRLGTGFAGHLAAGVGCFKYIVLDTPLLLAEDPVVGGYEASGAVYKFNNARGQGGFLNW 425

Query: 425 DLLPDAGGSP 432
           +LLP A   P
Sbjct: 426 NLLPCASQRP 435

BLAST of Moc04g39340 vs. ExPASy TrEMBL
Match: A0A5A7SRY5 (MuDRA-like transposase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold7507G00080 PE=4 SV=1)

HSP 1 Score: 697.2 bits (1798), Expect = 4.3e-197
Identity = 350/424 (82.55%), Postives = 386/424 (91.04%), Query Frame = 0

Query: 5   LSAALLPSSS-ALLPRLPRST-SRLCIASSHGGGVELVADSAAP-AQRVSFGFKNLADTF 64
           LSAALLPSSS  L  R+PR+T S+L I S H   VEL+AD   P +QR+SFGF+N+ADTF
Sbjct: 6   LSAALLPSSSFGLHLRIPRTTSSKLRILSIHATNVELIADPPTPSSQRLSFGFQNVADTF 65

Query: 65  WVDVQRAEGRPLSVGLNSPLHIGETKLEMVDNVAIRVELSNGCVGWGEVQVLPLVTDVNL 124
           WV+VQRAEGRPLS+GLNSPLH G +KLE +DNVA+RVELSNGCVGWGEVQVLP VTDV+L
Sbjct: 66  WVNVQRAEGRPLSIGLNSPLHFGNSKLETLDNVAMRVELSNGCVGWGEVQVLPSVTDVSL 125

Query: 125 ETALGKAEDICSYLRRTPPTTLNSVFDDIAGLLSPREFAPIRAGVEMALIDAVANSIDVP 184
           E AL KA+++C++LRRTPP TL+SVF+D+ GLLSPREFAPIRAGVEMALIDAVANSI+VP
Sbjct: 126 EMALAKAQEVCNFLRRTPPATLSSVFNDVTGLLSPREFAPIRAGVEMALIDAVANSINVP 185

Query: 185 LWRLFGGVTSTLTTAITVPIISPAEASLLASKYCNQGFETLKLVVGKNFAAEIAAIEAIK 244
           LWRLFGGVTSTLTT ITVPI+SP EAS+LASK+CNQGFETLKLVVGKNFAAEIAAIEAI 
Sbjct: 186 LWRLFGGVTSTLTTGITVPILSPEEASILASKFCNQGFETLKLVVGKNFAAEIAAIEAIH 245

Query: 245 EAQPCCSFMFDANEGYTPDEAIKFLKRLKDMGIVPLVFEQPVARDDWKGLREVSNLAREY 304
            AQPCCSFMFDANEGYTPDEAIKFL++LKD+GIVPLVFEQPV RDDWKGL+EVSN+AR +
Sbjct: 246 AAQPCCSFMFDANEGYTPDEAIKFLEKLKDVGIVPLVFEQPVDRDDWKGLQEVSNVARMH 305

Query: 305 GVPVAADESCRNSTDVRKIIDENLVDAINIKLPKFGVLGVLEIIELERKSGLILMIDSMA 364
           G+PVA DESCR+ TDV KIIDENLVDAINIKLPKFGVLG LEII L RKSGLILM+DSMA
Sbjct: 306 GIPVAVDESCRSLTDVCKIIDENLVDAINIKLPKFGVLGALEIINLARKSGLILMVDSMA 365

Query: 365 ETRLGTGFAGHLAAGSGCFKYIVLDAPFLLAEDPVVGGYEASGAVYKFNNARGQGGFLNW 424
           ETRLGTGFAGHLAAG GCFKYIVLD P LLAEDPVVGGYEASGAVYKFNNARGQGGFLNW
Sbjct: 366 ETRLGTGFAGHLAAGVGCFKYIVLDTPLLLAEDPVVGGYEASGAVYKFNNARGQGGFLNW 425

Query: 425 DLLP 426
           +LLP
Sbjct: 426 NLLP 429

BLAST of Moc04g39340 vs. ExPASy TrEMBL
Match: A0A5D3C439 (L-Ala-D/L-amino acid epimerase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold453G001880 PE=4 SV=1)

HSP 1 Score: 697.2 bits (1798), Expect = 4.3e-197
Identity = 350/424 (82.55%), Postives = 386/424 (91.04%), Query Frame = 0

Query: 5   LSAALLPSSS-ALLPRLPRST-SRLCIASSHGGGVELVADSAAP-AQRVSFGFKNLADTF 64
           LSAALLPSSS  L  R+PR+T S+L I S H   VEL+AD   P +QR+SFGF+N+ADTF
Sbjct: 6   LSAALLPSSSFGLHLRIPRTTSSKLRILSIHATNVELIADPPTPSSQRLSFGFQNVADTF 65

Query: 65  WVDVQRAEGRPLSVGLNSPLHIGETKLEMVDNVAIRVELSNGCVGWGEVQVLPLVTDVNL 124
           WV+VQRAEGRPLS+GLNSPLH G +KLE +DNVA+RVELSNGCVGWGEVQVLP VTDV+L
Sbjct: 66  WVNVQRAEGRPLSIGLNSPLHFGNSKLETLDNVAMRVELSNGCVGWGEVQVLPSVTDVSL 125

Query: 125 ETALGKAEDICSYLRRTPPTTLNSVFDDIAGLLSPREFAPIRAGVEMALIDAVANSIDVP 184
           E AL KA+++C++LRRTPP TL+SVF+D+ GLLSPREFAPIRAGVEMALIDAVANSI+VP
Sbjct: 126 EMALAKAQEVCNFLRRTPPATLSSVFNDVTGLLSPREFAPIRAGVEMALIDAVANSINVP 185

Query: 185 LWRLFGGVTSTLTTAITVPIISPAEASLLASKYCNQGFETLKLVVGKNFAAEIAAIEAIK 244
           LWRLFGGVTSTLTT ITVPI+SP EAS+LASK+CNQGFETLKLVVGKNFAAEIAAIEAI 
Sbjct: 186 LWRLFGGVTSTLTTGITVPILSPEEASILASKFCNQGFETLKLVVGKNFAAEIAAIEAIH 245

Query: 245 EAQPCCSFMFDANEGYTPDEAIKFLKRLKDMGIVPLVFEQPVARDDWKGLREVSNLAREY 304
            AQPCCSFMFDANEGYTPDEAIKFL++LKD+GIVPLVFEQPV RDDWKGL+EVSN+AR +
Sbjct: 246 AAQPCCSFMFDANEGYTPDEAIKFLEKLKDVGIVPLVFEQPVDRDDWKGLQEVSNVARMH 305

Query: 305 GVPVAADESCRNSTDVRKIIDENLVDAINIKLPKFGVLGVLEIIELERKSGLILMIDSMA 364
           G+PVA DESCR+ TDV KIIDENLVDAINIKLPKFGVLG LEII L RKSGLILM+DSMA
Sbjct: 306 GIPVAVDESCRSLTDVCKIIDENLVDAINIKLPKFGVLGALEIINLARKSGLILMVDSMA 365

Query: 365 ETRLGTGFAGHLAAGSGCFKYIVLDAPFLLAEDPVVGGYEASGAVYKFNNARGQGGFLNW 424
           ETRLGTGFAGHLAAG GCFKYIVLD P LLAEDPVVGGYEASGAVYKFNNARGQGGFLNW
Sbjct: 366 ETRLGTGFAGHLAAGVGCFKYIVLDTPLLLAEDPVVGGYEASGAVYKFNNARGQGGFLNW 425

Query: 425 DLLP 426
           +LLP
Sbjct: 426 NLLP 429

BLAST of Moc04g39340 vs. TAIR 10
Match: AT3G18270.1 (cytochrome P450, family 77, subfamily A, polypeptide 5 pseudogene )

HSP 1 Score: 415.6 bits (1067), Expect = 4.8e-116
Identity = 203/370 (54.86%), Postives = 266/370 (71.89%), Query Frame = 0

Query: 54  FKNLADTFWVDVQRAEGRPLSVGLNSPLHIGETKLEMVDNVAIRVELSNGCVGWGEVQVL 113
           FK L + F V V +AE R L+V L SP  I  ++L+ V NVAIR+EL++G VGWGE  +L
Sbjct: 37  FKTLTENFTVRVLKAENRELNVALLSPFTIASSRLDSVSNVAIRIELNDGFVGWGEAPIL 96

Query: 114 PLVTDVNLETALGKAEDICSYLRRTPPTTLNSVFDDIAGLLSPREFAPIRAGVEMALIDA 173
           P VT  +   A+ KA +   +LR  P   L +V  +I   L   +FA +RAG+EMA+IDA
Sbjct: 97  PSVTAEDQIMAMVKAREASEFLRELPEMKLGNVLQEIGRFLPGHQFASVRAGMEMAMIDA 156

Query: 174 VANSIDVPLWRLFGGVTSTLTTAITVPIISPAEASLLASKYCNQGFETLKLVVGKNFAAE 233
            A S+ VPLW+LFGG +ST+TT IT+PI+SPAEAS+LASKY  +GFETLKL VGKN  A+
Sbjct: 157 AAKSVRVPLWKLFGGASSTITTDITIPIVSPAEASVLASKYRKRGFETLKLKVGKNLKAD 216

Query: 234 IAAIEAIKEAQPCCSFMFDANEGYTPDEAIKFLKRLKDMGIVPLVFEQPVARDDWKGLRE 293
           I  ++AI+   P CSF+ DANEGY  +EA+K L+ L +M + P++FEQPV RD+W+GL  
Sbjct: 217 IEVLQAIRAVHPTCSFILDANEGYQTEEAVKVLETLHEMKVTPVLFEQPVHRDNWEGLSH 276

Query: 294 VSNLAR-EYGVPVAADESCRNSTDVRKIIDENLVDAINIKLPKFGVLGVLEIIELERKSG 353
           V+  A+  +GV +AADESCR  TD++KII+ N+VD +NIKL K G+L  LE+IEL R SG
Sbjct: 277 VTRTAKNRFGVSIAADESCRGLTDLKKIIEGNIVDVVNIKLAKTGILESLEVIELARSSG 336

Query: 354 LILMIDSMAETRLGTGFAGHLAAGSGCFKYIVLDAPFLLAEDPVVGGYEASGAVYKFNNA 413
           + LMI  M ETRL  GF+GHLAAG GCF++I LD P LLA+DPV GGY+A GAVY+F + 
Sbjct: 337 IELMIGGMVETRLAMGFSGHLAAGLGCFRFIDLDTPLLLADDPVQGGYKACGAVYEFKDE 396

Query: 414 RGQGGFLNWD 423
            G GG+L W+
Sbjct: 397 GGHGGYLQWN 406

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022133667.11.3e-243100.00L-Ala-D/L-amino acid epimerase-like [Momordica charantia][more]
XP_023550334.18.0e-20684.76L-Ala-D/L-amino acid epimerase-like [Cucurbita pepo subsp. pepo][more]
XP_038884241.11.2e-20483.91L-Ala-D/L-amino acid epimerase-like [Benincasa hispida][more]
KAG6578347.12.9e-20384.30L-Ala-D/L-amino acid epimerase, partial [Cucurbita argyrosperma subsp. sororia][more]
XP_022938632.11.1e-20284.45L-Ala-D/L-amino acid epimerase-like [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
B9I2J64.4e-12257.94L-Ala-D/L-amino acid epimerase OS=Populus trichocarpa OX=3694 GN=POPTR_0012s0504... [more]
A9B0558.7e-4632.11Aromatic dipeptide epimerase OS=Herpetosiphon aurantiacus (strain ATCC 23779 / D... [more]
B5EFW25.0e-4131.02Hydrophobic dipeptide epimerase OS=Geobacter bemidjiensis (strain Bem / ATCC BAA... [more]
O345081.1e-4031.92L-Ala-D/L-Glu epimerase OS=Bacillus subtilis (strain 168) OX=224308 GN=ykfB PE=1... [more]
Q9WXM11.0e-3832.00L-Ala-D/L-Glu epimerase OS=Thermotoga maritima (strain ATCC 43589 / DSM 3109 / J... [more]
Match NameE-valueIdentityDescription
A0A6J1BXC66.1e-244100.00L-Ala-D/L-amino acid epimerase-like OS=Momordica charantia OX=3673 GN=LOC1110061... [more]
A0A6J1FEM15.2e-20384.45L-Ala-D/L-amino acid epimerase-like OS=Cucurbita moschata OX=3662 GN=LOC11144480... [more]
A0A1S3B3261.5e-19781.86L-Ala-D/L-amino acid epimerase-like OS=Cucumis melo OX=3656 GN=LOC103485448 PE=4... [more]
A0A5A7SRY54.3e-19782.55MuDRA-like transposase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold7... [more]
A0A5D3C4394.3e-19782.55L-Ala-D/L-amino acid epimerase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5... [more]
Match NameE-valueIdentityDescription
AT3G18270.14.8e-11654.86cytochrome P450, family 77, subfamily A, polypeptide 5 pseudogene [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (OHB3-1) v2
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR013342Mandelate racemase/muconate lactonizing enzyme, C-terminalSMARTSM00922MR_MLE_2coord: 204..304
e-value: 2.8E-10
score: 50.1
IPR029017Enolase-like, N-terminalGENE3D3.30.390.10coord: 72..178
e-value: 6.8E-75
score: 254.1
IPR029017Enolase-like, N-terminalSUPERFAMILY54826Enolase N-terminal domain-likecoord: 65..188
IPR013341Mandelate racemase/muconate lactonizing enzyme, N-terminal domainPFAMPF02746MR_MLE_Ncoord: 78..187
e-value: 3.1E-7
score: 30.6
IPR029065Enolase C-terminal domain-likePFAMPF13378MR_MLE_Ccoord: 214..401
e-value: 1.2E-30
score: 106.8
IPR036849Enolase-like, C-terminal domain superfamilyGENE3D3.20.20.120coord: 179..406
e-value: 6.8E-75
score: 254.1
IPR036849Enolase-like, C-terminal domain superfamilySUPERFAMILY51604Enolase C-terminal domain-likecoord: 179..425
NoneNo IPR availableSFLDSFLDS00001Enolasecoord: 68..424
e-value: 0.0
score: 173.0
NoneNo IPR availablePANTHERPTHR48073:SF4DIPEPTIDE EPIMERASEcoord: 38..424
NoneNo IPR availablePANTHERPTHR48073O-SUCCINYLBENZOATE SYNTHASE-RELATEDcoord: 38..424

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Moc04g39340.1Moc04g39340.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0016854 racemase and epimerase activity
molecular_function GO:0008270 zinc ion binding