Moc04g38930 (gene) Bitter gourd (OHB3-1) v2

Overview
NameMoc04g38930
Typegene
OrganismMomordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
DescriptionCalcium-binding EF-hand family protein
Locationchr4: 29041424 .. 29041846 (+)
RNA-Seq ExpressionMoc04g38930
SyntenyMoc04g38930
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGACAAGCTCGCATTGTACGAGAAAGTGTTCAACCAGGTCGACGGCGACGGAGACGGGAAGCTGTCTCCGCCAGAGCTGCAGCAGTGCATGGCGGCCGTCGGGGGGAGTTTGACGCTCGAGGAGGCGGAGTTGGTGGTGGGGAATTTGGATTCCGACGGCGATGGGCTGGTGGGATGGGCTGAATTTGTTGCGTTTGTGGAAGGAGTGAAGGAGGAAGAGAAGGTGCGTGATCTGAAGGAGGCGTTCAGGATGTATGAAATGGATGGTTGTGGATTCATCACGACCAAGAGTCTGAAGAGGATGCTCAGTAGGCTTGGGGAATCGAGGAGCATTGAAGAATGCAAGAAAATGATTGCTAAATTTGATCTCAATTCTGATGGCGTTCTTAACTTTGATGAGTTCAAGTTCATGATGTCCTGA

mRNA sequence

ATGGACAAGCTCGCATTGTACGAGAAAGTGTTCAACCAGGTCGACGGCGACGGAGACGGGAAGCTGTCTCCGCCAGAGCTGCAGCAGTGCATGGCGGCCGTCGGGGGGAGTTTGACGCTCGAGGAGGCGGAGTTGGTGGTGGGGAATTTGGATTCCGACGGCGATGGGCTGGTGGGATGGGCTGAATTTGTTGCGTTTGTGGAAGGAGTGAAGGAGGAAGAGAAGGTGCGTGATCTGAAGGAGGCGTTCAGGATGTATGAAATGGATGGTTGTGGATTCATCACGACCAAGAGTCTGAAGAGGATGCTCAGTAGGCTTGGGGAATCGAGGAGCATTGAAGAATGCAAGAAAATGATTGCTAAATTTGATCTCAATTCTGATGGCGTTCTTAACTTTGATGAGTTCAAGTTCATGATGTCCTGA

Coding sequence (CDS)

ATGGACAAGCTCGCATTGTACGAGAAAGTGTTCAACCAGGTCGACGGCGACGGAGACGGGAAGCTGTCTCCGCCAGAGCTGCAGCAGTGCATGGCGGCCGTCGGGGGGAGTTTGACGCTCGAGGAGGCGGAGTTGGTGGTGGGGAATTTGGATTCCGACGGCGATGGGCTGGTGGGATGGGCTGAATTTGTTGCGTTTGTGGAAGGAGTGAAGGAGGAAGAGAAGGTGCGTGATCTGAAGGAGGCGTTCAGGATGTATGAAATGGATGGTTGTGGATTCATCACGACCAAGAGTCTGAAGAGGATGCTCAGTAGGCTTGGGGAATCGAGGAGCATTGAAGAATGCAAGAAAATGATTGCTAAATTTGATCTCAATTCTGATGGCGTTCTTAACTTTGATGAGTTCAAGTTCATGATGTCCTGA

Protein sequence

MDKLALYEKVFNQVDGDGDGKLSPPELQQCMAAVGGSLTLEEAELVVGNLDSDGDGLVGWAEFVAFVEGVKEEEKVRDLKEAFRMYEMDGCGFITTKSLKRMLSRLGESRSIEECKKMIAKFDLNSDGVLNFDEFKFMMS
Homology
[Close]
BLAST of Moc04g38930 vs. NCBI nr
Match: XP_022133861.1 (putative calcium-binding protein CML19 [Momordica charantia])

HSP 1 Score: 281.2 bits (718), Expect = 4.9e-72
Identity = 140/140 (100.00%), Postives = 140/140 (100.00%), Query Frame = 0 

Query: 1   MDKLALYEKVFNQVDGDGDGKLSPPELQQCMAAVGGSLTLEEAELVVGNLDSDGDGLVGW 60
           MDKLALYEKVFNQVDGDGDGKLSPPELQQCMAAVGGSLTLEEAELVVGNLDSDGDGLVGW
Sbjct: 1   MDKLALYEKVFNQVDGDGDGKLSPPELQQCMAAVGGSLTLEEAELVVGNLDSDGDGLVGW 60

Query: 61  AEFVAFVEGVKEEEKVRDLKEAFRMYEMDGCGFITTKSLKRMLSRLGESRSIEECKKMIA 120
           AEFVAFVEGVKEEEKVRDLKEAFRMYEMDGCGFITTKSLKRMLSRLGESRSIEECKKMIA
Sbjct: 61  AEFVAFVEGVKEEEKVRDLKEAFRMYEMDGCGFITTKSLKRMLSRLGESRSIEECKKMIA 120

Query: 121 KFDLNSDGVLNFDEFKFMMS 141
           KFDLNSDGVLNFDEFKFMMS
Sbjct: 121 KFDLNSDGVLNFDEFKFMMS 140

[Close]
BLAST of Moc04g38930 vs. NCBI nr
Match: XP_022955599.1 (putative calcium-binding protein CML19 [Cucurbita moschata] >XP_022955608.1 putative calcium-binding protein CML19 [Cucurbita moschata] >KAG6602674.1 putative calcium-binding protein CML19, partial [Cucurbita argyrosperma subsp. sororia] >KAG7033361.1 putative calcium-binding protein CML19, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 255.0 bits (650), Expect = 3.8e-64
Identity = 128/140 (91.43%), Postives = 131/140 (93.57%), Query Frame = 0 

Query: 1   MDKLALYEKVFNQVDGDGDGKLSPPELQQCMAAVGGSLTLEEAELVVGNLDSDGDGLVGW 60
           MDK+ALYEKVF QVDGDGDGKLS PELQ+CMA VGGSLTLEEAE VV NLDSDGDGLV W
Sbjct: 1   MDKIALYEKVFKQVDGDGDGKLSLPELQRCMAVVGGSLTLEEAEKVVENLDSDGDGLVSW 60

Query: 61  AEFVAFVEGVKEEEKVRDLKEAFRMYEMDGCGFITTKSLKRMLSRLGESRSIEECKKMIA 120
           AEFVAF+EGV EEEKV DL EAFRMYEMDGCGFITTKSLKRMLSRLGESRSIEECKKMIA
Sbjct: 61  AEFVAFMEGVGEEEKVSDLMEAFRMYEMDGCGFITTKSLKRMLSRLGESRSIEECKKMIA 120

Query: 121 KFDLNSDGVLNFDEFKFMMS 141
           KFDLNSDGVLNFDEFKFMMS
Sbjct: 121 KFDLNSDGVLNFDEFKFMMS 140

[Close]
BLAST of Moc04g38930 vs. NCBI nr
Match: XP_022938684.1 (putative calcium-binding protein CML19 [Cucurbita moschata] >XP_023550776.1 putative calcium-binding protein CML19 isoform X2 [Cucurbita pepo subsp. pepo] >KAG7015957.1 putative calcium-binding protein CML19, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 254.2 bits (648), Expect = 6.5e-64
Identity = 126/140 (90.00%), Postives = 131/140 (93.57%), Query Frame = 0 

Query: 1   MDKLALYEKVFNQVDGDGDGKLSPPELQQCMAAVGGSLTLEEAELVVGNLDSDGDGLVGW 60
           MDK+ALYEKVF QVDGDGDGKLSP ELQ+CMA VGGSLTLEEAE+VV NLD+DGDGL+GW
Sbjct: 2   MDKMALYEKVFKQVDGDGDGKLSPLELQRCMAGVGGSLTLEEAEMVVENLDTDGDGLMGW 61

Query: 61  AEFVAFVEGVKEEEKVRDLKEAFRMYEMDGCGFITTKSLKRMLSRLGESRSIEECKKMIA 120
            EFVAFVEGV EEEKV DLKEAFRMYEMDGCGFITTKSLKRMLSRLGESRSIEEC KMIA
Sbjct: 62  GEFVAFVEGVGEEEKVSDLKEAFRMYEMDGCGFITTKSLKRMLSRLGESRSIEECNKMIA 121

Query: 121 KFDLNSDGVLNFDEFKFMMS 141
           KFDLNSDGVLNFDEF FMMS
Sbjct: 122 KFDLNSDGVLNFDEFMFMMS 141

[Close]
BLAST of Moc04g38930 vs. NCBI nr
Match: KAG6578374.1 (Protoporphyrinogen oxidase 2, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 254.2 bits (648), Expect = 6.5e-64
Identity = 126/140 (90.00%), Postives = 131/140 (93.57%), Query Frame = 0 

Query: 1    MDKLALYEKVFNQVDGDGDGKLSPPELQQCMAAVGGSLTLEEAELVVGNLDSDGDGLVGW 60
            MDK+ALYEKVF QVDGDGDGKLSP ELQ+CMA VGGSLTLEEAE+VV NLD+DGDGL+GW
Sbjct: 1309 MDKMALYEKVFKQVDGDGDGKLSPLELQRCMAGVGGSLTLEEAEMVVENLDTDGDGLMGW 1368

Query: 61   AEFVAFVEGVKEEEKVRDLKEAFRMYEMDGCGFITTKSLKRMLSRLGESRSIEECKKMIA 120
             EFVAFVEGV EEEKV DLKEAFRMYEMDGCGFITTKSLKRMLSRLGESRSIEEC KMIA
Sbjct: 1369 GEFVAFVEGVGEEEKVSDLKEAFRMYEMDGCGFITTKSLKRMLSRLGESRSIEECNKMIA 1428

Query: 121  KFDLNSDGVLNFDEFKFMMS 141
            KFDLNSDGVLNFDEF FMMS
Sbjct: 1429 KFDLNSDGVLNFDEFMFMMS 1448

[Close]
BLAST of Moc04g38930 vs. NCBI nr
Match: XP_023550775.1 (putative calcium-binding protein CML19 isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 254.2 bits (648), Expect = 6.5e-64
Identity = 126/140 (90.00%), Postives = 131/140 (93.57%), Query Frame = 0 

Query: 1   MDKLALYEKVFNQVDGDGDGKLSPPELQQCMAAVGGSLTLEEAELVVGNLDSDGDGLVGW 60
           MDK+ALYEKVF QVDGDGDGKLSP ELQ+CMA VGGSLTLEEAE+VV NLD+DGDGL+GW
Sbjct: 58  MDKMALYEKVFKQVDGDGDGKLSPLELQRCMAGVGGSLTLEEAEMVVENLDTDGDGLMGW 117

Query: 61  AEFVAFVEGVKEEEKVRDLKEAFRMYEMDGCGFITTKSLKRMLSRLGESRSIEECKKMIA 120
            EFVAFVEGV EEEKV DLKEAFRMYEMDGCGFITTKSLKRMLSRLGESRSIEEC KMIA
Sbjct: 118 GEFVAFVEGVGEEEKVSDLKEAFRMYEMDGCGFITTKSLKRMLSRLGESRSIEECNKMIA 177

Query: 121 KFDLNSDGVLNFDEFKFMMS 141
           KFDLNSDGVLNFDEF FMMS
Sbjct: 178 KFDLNSDGVLNFDEFMFMMS 197

[Close]
BLAST of Moc04g38930 vs. ExPASy Swiss-Prot
Match: Q8RYJ8 (Putative calcium-binding protein CML19 OS=Oryza sativa subsp. japonica OX=39947 GN=CML19 PE=3 SV=1)

HSP 1 Score: 134.4 bits (337), Expect = 9.8e-31
Identity = 73/139 (52.52%), Postives = 96/139 (69.06%), Query Frame = 0 

Query: 5   ALYEKVFNQVDGDGDGKLSPPELQQCM-AAVGGSLTLEEAELVVGNLDSDGDGLVGWAEF 64
           A + +VF+  D D DGK+S  EL+ CM AA+G  ++ EEAE +V + D+D DGL+   EF
Sbjct: 6   AEFRRVFSAFDRDADGKISAAELRLCMKAALGEDMSAEEAEALVSSADTDDDGLLDEEEF 65

Query: 65  VAF---VEGVKEEEKVRDLKEAFRMYEMDGCGFITTKSLKRMLSRLGESRSIEECKKMIA 124
                 +E   EEE+ R L EAFRMYEM+G G IT  SLKRMLS+LG  + IEEC+ MI 
Sbjct: 66  TKLAVQLEMGDEEERCRGLMEAFRMYEMEGEGRITPASLKRMLSKLGSHQGIEECQTMIC 125

Query: 125 KFDLNSDGVLNFDEFKFMM 140
           +FDL+ DGV++F+EFK MM
Sbjct: 126 RFDLDGDGVISFEEFKIMM 144

[Close]
BLAST of Moc04g38930 vs. ExPASy Swiss-Prot
Match: Q9FIH9 (Calcium-binding protein CML37 OS=Arabidopsis thaliana OX=3702 GN=CML37 PE=1 SV=1)

HSP 1 Score: 130.6 bits (327), Expect = 1.4e-29
Identity = 68/132 (51.52%), Postives = 91/132 (68.94%), Query Frame = 0 

Query: 10  VFNQVDGDGDGKLSPPELQQCMAAVGGSLTLEEAELVVGNLDSDGDGLVGWAEFVAFVEG 69
           VF+ +D + DGK+S  ELQ C++ +GG+L+  E E VV   D DGDG + + EF+  +EG
Sbjct: 53  VFDYMDANSDGKISGEELQSCVSLLGGALSSREVEEVVKTSDVDGDGFIDFEEFLKLMEG 112

Query: 70  V--KEEEKVRDLKEAFRMYEMDGCGFITTKSLKRMLSRLGESRSIEECKKMIAKFDLNSD 129
               +EE+ ++LKEAF MY M+G  FIT  SL+R LSRLGES +++ CK MI  FD N D
Sbjct: 113 EDGSDEERRKELKEAFGMYVMEGEEFITAASLRRTLSRLGESCTVDACKVMIRGFDQNDD 172

Query: 130 GVLNFDEFKFMM 140
           GVL+FDEF  MM
Sbjct: 173 GVLSFDEFVLMM 184

[Close]
BLAST of Moc04g38930 vs. ExPASy Swiss-Prot
Match: Q8RYK0 (Probable calcium-binding protein CML31 OS=Oryza sativa subsp. japonica OX=39947 GN=CML31 PE=2 SV=1)

HSP 1 Score: 126.7 bits (317), Expect = 2.0e-28
Identity = 70/136 (51.47%), Postives = 96/136 (70.59%), Query Frame = 0 

Query: 10  VFNQVDGDGDGKLSPPELQQCM-AAVGGSLTLEEAELVVGNLDSDGDGLVGWAEFVAFVE 69
           +F   D DGDG++S  EL+ CM   +G  ++ EEA  +V ++D+DGDGL+  AEFV  V+
Sbjct: 15  LFATFDHDGDGRISAAELRLCMKTTLGEEVSDEEAGQLVASVDADGDGLLCEAEFVRLVQ 74

Query: 70  G--VKEEEKVR--DLKEAFRMYEMDGCGFITTKSLKRMLSRLGESRSIEECKKMIAKFDL 129
              V+EE++ R   L+EAF MYEM+G G IT  SL+RML RLG  + I++C+ MI +FDL
Sbjct: 75  AAEVEEEDERRGTGLREAFGMYEMEGEGCITPTSLRRMLRRLGSDQDIDDCRAMICRFDL 134

Query: 130 NSDGVLNFDEFKFMMS 141
           N DGVL+FDEFK MM+
Sbjct: 135 NGDGVLSFDEFKIMMN 150

[Close]
BLAST of Moc04g38930 vs. ExPASy Swiss-Prot
Match: Q9SRE6 (Calcium-binding protein CML38 OS=Arabidopsis thaliana OX=3702 GN=CML38 PE=1 SV=1)

HSP 1 Score: 125.6 bits (314), Expect = 4.5e-28
Identity = 66/138 (47.83%), Postives = 92/138 (66.67%), Query Frame = 0 

Query: 2   DKLALYEKVFNQVDGDGDGKLSPPELQQCMAAVGGSLTLEEAELVVGNLDSDGDGLVGWA 61
           DK    E VF+ +D + DG++SP ELQ+    +G  L+ EEA   V   D+DGDG++ + 
Sbjct: 39  DKNRELEAVFSYMDANRDGRISPEELQKSFMTLGEQLSDEEAVAAVRLSDTDGDGMLDFE 98

Query: 62  EFVAFVEGVKEEEKVRDLKEAFRMYEMDGCGFITTKSLKRMLSRLGESRSIEECKKMIAK 121
           EF   ++   EEEK  +LK AFR+Y  +G   IT +SLK ML +LGESR+ ++C+ MI+ 
Sbjct: 99  EFSQLIKVDDEEEKKMELKGAFRLYIAEGEDCITPRSLKMMLKKLGESRTTDDCRVMISA 158

Query: 122 FDLNSDGVLNFDEFKFMM 140
           FDLN+DGVL+FDEF  MM
Sbjct: 159 FDLNADGVLSFDEFALMM 176

[Close]
BLAST of Moc04g38930 vs. ExPASy Swiss-Prot
Match: Q8RYJ9 (Putative calcium-binding protein CML23 OS=Oryza sativa subsp. japonica OX=39947 GN=CML23 PE=3 SV=1)

HSP 1 Score: 120.6 bits (301), Expect = 1.5e-26
Identity = 69/143 (48.25%), Postives = 91/143 (63.64%), Query Frame = 0 

Query: 7   YEKVFNQVDGDGDGKLSPPELQQCM-AAVGGSLTLEEAELVVGNLDSDGDGLVGWAEFVA 66
           + +VF   D DGDGK+S  EL+ C+ A++G  +  EE + ++   D+DGDGL+   EFV 
Sbjct: 7   FRRVFGSFDQDGDGKISATELRLCVKASLGEDMPDEEVQALMALADTDGDGLLDEEEFVR 66

Query: 67  FV-----EGVKEE----EKVRDLKEAFRMYEMDGCGFITTKSLKRMLSRLGESRSIEECK 126
            V     +G +EE    E  R L+EAF MYEM+G G IT  SLK MLS+LG    + EC+
Sbjct: 67  LVTEMEADGDEEEDDDDETCRCLREAFAMYEMEGRGCITPLSLKLMLSKLGTHLDVAECQ 126

Query: 127 KMIAKFDLNSDGVLNFDEFKFMM 140
            MI +FD+N DGVL FDEFK MM
Sbjct: 127 AMICRFDMNGDGVLTFDEFKTMM 149

[Close]
BLAST of Moc04g38930 vs. ExPASy TrEMBL
Match: A0A6J1BWC8 (putative calcium-binding protein CML19 OS=Momordica charantia OX=3673 GN=LOC111006309 PE=4 SV=1)

HSP 1 Score: 281.2 bits (718), Expect = 2.4e-72
Identity = 140/140 (100.00%), Postives = 140/140 (100.00%), Query Frame = 0 

Query: 1   MDKLALYEKVFNQVDGDGDGKLSPPELQQCMAAVGGSLTLEEAELVVGNLDSDGDGLVGW 60
           MDKLALYEKVFNQVDGDGDGKLSPPELQQCMAAVGGSLTLEEAELVVGNLDSDGDGLVGW
Sbjct: 1   MDKLALYEKVFNQVDGDGDGKLSPPELQQCMAAVGGSLTLEEAELVVGNLDSDGDGLVGW 60

Query: 61  AEFVAFVEGVKEEEKVRDLKEAFRMYEMDGCGFITTKSLKRMLSRLGESRSIEECKKMIA 120
           AEFVAFVEGVKEEEKVRDLKEAFRMYEMDGCGFITTKSLKRMLSRLGESRSIEECKKMIA
Sbjct: 61  AEFVAFVEGVKEEEKVRDLKEAFRMYEMDGCGFITTKSLKRMLSRLGESRSIEECKKMIA 120

Query: 121 KFDLNSDGVLNFDEFKFMMS 141
           KFDLNSDGVLNFDEFKFMMS
Sbjct: 121 KFDLNSDGVLNFDEFKFMMS 140

[Close]
BLAST of Moc04g38930 vs. ExPASy TrEMBL
Match: A0A6J1GWQ9 (putative calcium-binding protein CML19 OS=Cucurbita moschata OX=3662 GN=LOC111457555 PE=4 SV=1)

HSP 1 Score: 255.0 bits (650), Expect = 1.8e-64
Identity = 128/140 (91.43%), Postives = 131/140 (93.57%), Query Frame = 0 

Query: 1   MDKLALYEKVFNQVDGDGDGKLSPPELQQCMAAVGGSLTLEEAELVVGNLDSDGDGLVGW 60
           MDK+ALYEKVF QVDGDGDGKLS PELQ+CMA VGGSLTLEEAE VV NLDSDGDGLV W
Sbjct: 1   MDKIALYEKVFKQVDGDGDGKLSLPELQRCMAVVGGSLTLEEAEKVVENLDSDGDGLVSW 60

Query: 61  AEFVAFVEGVKEEEKVRDLKEAFRMYEMDGCGFITTKSLKRMLSRLGESRSIEECKKMIA 120
           AEFVAF+EGV EEEKV DL EAFRMYEMDGCGFITTKSLKRMLSRLGESRSIEECKKMIA
Sbjct: 61  AEFVAFMEGVGEEEKVSDLMEAFRMYEMDGCGFITTKSLKRMLSRLGESRSIEECKKMIA 120

Query: 121 KFDLNSDGVLNFDEFKFMMS 141
           KFDLNSDGVLNFDEFKFMMS
Sbjct: 121 KFDLNSDGVLNFDEFKFMMS 140

[Close]
BLAST of Moc04g38930 vs. ExPASy TrEMBL
Match: A0A6J1FET5 (putative calcium-binding protein CML19 OS=Cucurbita moschata OX=3662 GN=LOC111444836 PE=4 SV=1)

HSP 1 Score: 254.2 bits (648), Expect = 3.1e-64
Identity = 126/140 (90.00%), Postives = 131/140 (93.57%), Query Frame = 0 

Query: 1   MDKLALYEKVFNQVDGDGDGKLSPPELQQCMAAVGGSLTLEEAELVVGNLDSDGDGLVGW 60
           MDK+ALYEKVF QVDGDGDGKLSP ELQ+CMA VGGSLTLEEAE+VV NLD+DGDGL+GW
Sbjct: 2   MDKMALYEKVFKQVDGDGDGKLSPLELQRCMAGVGGSLTLEEAEMVVENLDTDGDGLMGW 61

Query: 61  AEFVAFVEGVKEEEKVRDLKEAFRMYEMDGCGFITTKSLKRMLSRLGESRSIEECKKMIA 120
            EFVAFVEGV EEEKV DLKEAFRMYEMDGCGFITTKSLKRMLSRLGESRSIEEC KMIA
Sbjct: 62  GEFVAFVEGVGEEEKVSDLKEAFRMYEMDGCGFITTKSLKRMLSRLGESRSIEECNKMIA 121

Query: 121 KFDLNSDGVLNFDEFKFMMS 141
           KFDLNSDGVLNFDEF FMMS
Sbjct: 122 KFDLNSDGVLNFDEFMFMMS 141

[Close]
BLAST of Moc04g38930 vs. ExPASy TrEMBL
Match: A0A6J1JZS3 (putative calcium-binding protein CML19 OS=Cucurbita maxima OX=3661 GN=LOC111488916 PE=4 SV=1)

HSP 1 Score: 253.8 bits (647), Expect = 4.1e-64
Identity = 126/140 (90.00%), Postives = 131/140 (93.57%), Query Frame = 0 

Query: 1   MDKLALYEKVFNQVDGDGDGKLSPPELQQCMAAVGGSLTLEEAELVVGNLDSDGDGLVGW 60
           MDK+ALYEKVF QVDGDGDGKLSP ELQ+CMA VGGSLTLEEAE+ V NLD+DGDGL+GW
Sbjct: 2   MDKMALYEKVFKQVDGDGDGKLSPLELQRCMAGVGGSLTLEEAEMAVENLDTDGDGLMGW 61

Query: 61  AEFVAFVEGVKEEEKVRDLKEAFRMYEMDGCGFITTKSLKRMLSRLGESRSIEECKKMIA 120
            EFVAFVE V EEEKV DLKEAFRMYEMDGCGFITTKSLKRMLSRLGESRSIEECKKMIA
Sbjct: 62  GEFVAFVEEVGEEEKVSDLKEAFRMYEMDGCGFITTKSLKRMLSRLGESRSIEECKKMIA 121

Query: 121 KFDLNSDGVLNFDEFKFMMS 141
           KFDLNSDGVLNFDEFKFMMS
Sbjct: 122 KFDLNSDGVLNFDEFKFMMS 141

[Close]
BLAST of Moc04g38930 vs. ExPASy TrEMBL
Match: A0A6J1JS06 (putative calcium-binding protein CML19 OS=Cucurbita maxima OX=3661 GN=LOC111487817 PE=4 SV=1)

HSP 1 Score: 251.9 bits (642), Expect = 1.6e-63
Identity = 127/140 (90.71%), Postives = 130/140 (92.86%), Query Frame = 0 

Query: 1   MDKLALYEKVFNQVDGDGDGKLSPPELQQCMAAVGGSLTLEEAELVVGNLDSDGDGLVGW 60
           MDK+ALYEKVF QVDGDGDGKLS PELQ+CMA VGGSLTLEEAE VV NLDSDGDGLV W
Sbjct: 1   MDKIALYEKVFKQVDGDGDGKLSLPELQRCMAVVGGSLTLEEAEKVVENLDSDGDGLVSW 60

Query: 61  AEFVAFVEGVKEEEKVRDLKEAFRMYEMDGCGFITTKSLKRMLSRLGESRSIEECKKMIA 120
           AEFVAF+E V EEEKV DL EAFRMYEMDGCGFITTKSLKRMLSRLGESRSIEECKKMIA
Sbjct: 61  AEFVAFMEEVGEEEKVSDLMEAFRMYEMDGCGFITTKSLKRMLSRLGESRSIEECKKMIA 120

Query: 121 KFDLNSDGVLNFDEFKFMMS 141
           KFDLNSDGVLNFDEFKFMMS
Sbjct: 121 KFDLNSDGVLNFDEFKFMMS 140

[Close]
BLAST of Moc04g38930 vs. TAIR 10
Match: AT5G42380.1 (calmodulin like 37 )

HSP 1 Score: 130.6 bits (327), Expect = 1.0e-30
Identity = 68/132 (51.52%), Postives = 91/132 (68.94%), Query Frame = 0 

Query: 10  VFNQVDGDGDGKLSPPELQQCMAAVGGSLTLEEAELVVGNLDSDGDGLVGWAEFVAFVEG 69
           VF+ +D + DGK+S  ELQ C++ +GG+L+  E E VV   D DGDG + + EF+  +EG
Sbjct: 53  VFDYMDANSDGKISGEELQSCVSLLGGALSSREVEEVVKTSDVDGDGFIDFEEFLKLMEG 112

Query: 70  V--KEEEKVRDLKEAFRMYEMDGCGFITTKSLKRMLSRLGESRSIEECKKMIAKFDLNSD 129
               +EE+ ++LKEAF MY M+G  FIT  SL+R LSRLGES +++ CK MI  FD N D
Sbjct: 113 EDGSDEERRKELKEAFGMYVMEGEEFITAASLRRTLSRLGESCTVDACKVMIRGFDQNDD 172

Query: 130 GVLNFDEFKFMM 140
           GVL+FDEF  MM
Sbjct: 173 GVLSFDEFVLMM 184

[Close]
BLAST of Moc04g38930 vs. TAIR 10
Match: AT1G76650.1 (calmodulin-like 38 )

HSP 1 Score: 125.6 bits (314), Expect = 3.2e-29
Identity = 66/138 (47.83%), Postives = 92/138 (66.67%), Query Frame = 0 

Query: 2   DKLALYEKVFNQVDGDGDGKLSPPELQQCMAAVGGSLTLEEAELVVGNLDSDGDGLVGWA 61
           DK    E VF+ +D + DG++SP ELQ+    +G  L+ EEA   V   D+DGDG++ + 
Sbjct: 39  DKNRELEAVFSYMDANRDGRISPEELQKSFMTLGEQLSDEEAVAAVRLSDTDGDGMLDFE 98

Query: 62  EFVAFVEGVKEEEKVRDLKEAFRMYEMDGCGFITTKSLKRMLSRLGESRSIEECKKMIAK 121
           EF   ++   EEEK  +LK AFR+Y  +G   IT +SLK ML +LGESR+ ++C+ MI+ 
Sbjct: 99  EFSQLIKVDDEEEKKMELKGAFRLYIAEGEDCITPRSLKMMLKKLGESRTTDDCRVMISA 158

Query: 122 FDLNSDGVLNFDEFKFMM 140
           FDLN+DGVL+FDEF  MM
Sbjct: 159 FDLNADGVLSFDEFALMM 176

[Close]
BLAST of Moc04g38930 vs. TAIR 10
Match: AT1G76650.3 (calmodulin-like 38 )

HSP 1 Score: 125.6 bits (314), Expect = 3.2e-29
Identity = 66/138 (47.83%), Postives = 92/138 (66.67%), Query Frame = 0 

Query: 2   DKLALYEKVFNQVDGDGDGKLSPPELQQCMAAVGGSLTLEEAELVVGNLDSDGDGLVGWA 61
           DK    E VF+ +D + DG++SP ELQ+    +G  L+ EEA   V   D+DGDG++ + 
Sbjct: 39  DKNRELEAVFSYMDANRDGRISPEELQKSFMTLGEQLSDEEAVAAVRLSDTDGDGMLDFE 98

Query: 62  EFVAFVEGVKEEEKVRDLKEAFRMYEMDGCGFITTKSLKRMLSRLGESRSIEECKKMIAK 121
           EF   ++   EEEK  +LK AFR+Y  +G   IT +SLK ML +LGESR+ ++C+ MI+ 
Sbjct: 99  EFSQLIKVDDEEEKKMELKGAFRLYIAEGEDCITPRSLKMMLKKLGESRTTDDCRVMISA 158

Query: 122 FDLNSDGVLNFDEFKFMM 140
           FDLN+DGVL+FDEF  MM
Sbjct: 159 FDLNADGVLSFDEFALMM 176

[Close]
BLAST of Moc04g38930 vs. TAIR 10
Match: AT1G76640.1 (Calcium-binding EF-hand family protein )

HSP 1 Score: 118.2 bits (295), Expect = 5.2e-27
Identity = 66/135 (48.89%), Postives = 86/135 (63.70%), Query Frame = 0 

Query: 8   EKVFNQVDGDGDGKLSPPELQQCMAAVGGSLTLEEAELVVGNLDSDGDGLVGWAEFVAFV 67
           E VF  +D + DG++S  EL++    +G  ++ EEAE  V   D DGDG++   EF   +
Sbjct: 24  EAVFAYMDANRDGRISAEELKKSFKTLGEQMSDEEAEAAVKLSDIDGDGMLDINEFALLI 83

Query: 68  EG---VKEEEKVRDLKEAFRMYEMDGCGFITTKSLKRMLSRLGESRSIEECKKMIAKFDL 127
           +G     EEEK R + EAFRMY  DG   IT  SLK ML +LGESR+ ++CK MI  FDL
Sbjct: 84  KGNDEFTEEEKKRKIMEAFRMYIADGEDCITPGSLKMMLMKLGESRTTDDCKVMIQAFDL 143

Query: 128 NSDGVLNFDEFKFMM 140
           N+DGVL+FDEF  MM
Sbjct: 144 NADGVLSFDEFALMM 158

[Close]
BLAST of Moc04g38930 vs. TAIR 10
Match: AT1G76650.2 (calmodulin-like 38 )

HSP 1 Score: 114.4 bits (285), Expect = 7.4e-26
Identity = 62/138 (44.93%), Postives = 87/138 (63.04%), Query Frame = 0 

Query: 2   DKLALYEKVFNQVDGDGDGKLSPPELQQCMAAVGGSLTLEEAELVVGNLDSDGDGLVGWA 61
           DK    E VF+ +D + DG++SP ELQ+    +G  L            D+DGDG++ + 
Sbjct: 39  DKNRELEAVFSYMDANRDGRISPEELQKSFMTLGEQLL----------SDTDGDGMLDFE 98

Query: 62  EFVAFVEGVKEEEKVRDLKEAFRMYEMDGCGFITTKSLKRMLSRLGESRSIEECKKMIAK 121
           EF   ++   EEEK  +LK AFR+Y  +G   IT +SLK ML +LGESR+ ++C+ MI+ 
Sbjct: 99  EFSQLIKVDDEEEKKMELKGAFRLYIAEGEDCITPRSLKMMLKKLGESRTTDDCRVMISA 158

Query: 122 FDLNSDGVLNFDEFKFMM 140
           FDLN+DGVL+FDEF  MM
Sbjct: 159 FDLNADGVLSFDEFALMM 166

The following BLAST results are available for this feature:
BLAST of Moc04g38930 vs. NCBI nr
Match NameE-valueIdentityDescription
XP_022133861.14.9e-72100.00putative calcium-binding protein CML19 [Momordica charantia][more]
XP_022955599.13.8e-6491.43putative calcium-binding protein CML19 [Cucurbita moschata] >XP_022955608.1 puta... [more]
XP_022938684.16.5e-6490.00putative calcium-binding protein CML19 [Cucurbita moschata] >XP_023550776.1 puta... [more]
KAG6578374.16.5e-6490.00Protoporphyrinogen oxidase 2, partial [Cucurbita argyrosperma subsp. sororia][more]
XP_023550775.16.5e-6490.00putative calcium-binding protein CML19 isoform X1 [Cucurbita pepo subsp. pepo][more]
BLAST of Moc04g38930 vs. ExPASy Swiss-Prot
Match NameE-valueIdentityDescription
Q8RYJ89.8e-3152.52Putative calcium-binding protein CML19 OS=Oryza sativa subsp. japonica OX=39947 ... [more]
Q9FIH91.4e-2951.52Calcium-binding protein CML37 OS=Arabidopsis thaliana OX=3702 GN=CML37 PE=1 SV=1[more]
Q8RYK02.0e-2851.47Probable calcium-binding protein CML31 OS=Oryza sativa subsp. japonica OX=39947 ... [more]
Q9SRE64.5e-2847.83Calcium-binding protein CML38 OS=Arabidopsis thaliana OX=3702 GN=CML38 PE=1 SV=1[more]
Q8RYJ91.5e-2648.25Putative calcium-binding protein CML23 OS=Oryza sativa subsp. japonica OX=39947 ... [more]
BLAST of Moc04g38930 vs. ExPASy TrEMBL
Match NameE-valueIdentityDescription
A0A6J1BWC82.4e-72100.00putative calcium-binding protein CML19 OS=Momordica charantia OX=3673 GN=LOC1110... [more]
A0A6J1GWQ91.8e-6491.43putative calcium-binding protein CML19 OS=Cucurbita moschata OX=3662 GN=LOC11145... [more]
A0A6J1FET53.1e-6490.00putative calcium-binding protein CML19 OS=Cucurbita moschata OX=3662 GN=LOC11144... [more]
A0A6J1JZS34.1e-6490.00putative calcium-binding protein CML19 OS=Cucurbita maxima OX=3661 GN=LOC1114889... [more]
A0A6J1JS061.6e-6390.71putative calcium-binding protein CML19 OS=Cucurbita maxima OX=3661 GN=LOC1114878... [more]
BLAST of Moc04g38930 vs. TAIR 10
Match NameE-valueIdentityDescription
AT5G42380.11.0e-3051.52calmodulin like 37 [more]
AT1G76650.13.2e-2947.83calmodulin-like 38 [more]
AT1G76650.33.2e-2947.83calmodulin-like 38 [more]
AT1G76640.15.2e-2748.89Calcium-binding EF-hand family protein [more]
AT1G76650.27.4e-2644.93calmodulin-like 38 [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (OHB3-1) v2
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002048EF-hand domainSMARTSM00054efh_1coord: 78..106
e-value: 0.28
score: 20.2
coord: 114..140
e-value: 0.26
score: 20.3
coord: 42..70
e-value: 72.0
score: 2.8
coord: 6..34
e-value: 0.0056
score: 25.9
IPR002048EF-hand domainPFAMPF13499EF-hand_7coord: 6..67
e-value: 6.7E-12
score: 45.7
coord: 78..139
e-value: 5.2E-11
score: 42.8
IPR002048EF-hand domainPROSITEPS50222EF_HAND_2coord: 110..140
score: 14.318138
IPR002048EF-hand domainPROSITEPS50222EF_HAND_2coord: 74..109
score: 11.33333
IPR002048EF-hand domainPROSITEPS50222EF_HAND_2coord: 2..37
score: 13.202322
IPR002048EF-hand domainPROSITEPS50222EF_HAND_2coord: 38..73
score: 9.603815
IPR002048EF-hand domainCDDcd00051EFhcoord: 78..140
e-value: 1.4833E-15
score: 64.4913
NoneNo IPR availableGENE3D1.10.238.10coord: 1..70
e-value: 3.2E-15
score: 58.1
NoneNo IPR availableGENE3D1.10.238.10coord: 71..140
e-value: 9.8E-20
score: 72.6
NoneNo IPR availablePANTHERPTHR10891:SF858EF HAND CALCIUM-BINDING FAMILY PROTEINcoord: 2..140
NoneNo IPR availablePANTHERPTHR10891EF-HAND CALCIUM-BINDING DOMAIN CONTAINING PROTEINcoord: 2..140
IPR018247EF-Hand 1, calcium-binding sitePROSITEPS00018EF_HAND_1coord: 15..27
IPR018247EF-Hand 1, calcium-binding sitePROSITEPS00018EF_HAND_1coord: 51..63
IPR018247EF-Hand 1, calcium-binding sitePROSITEPS00018EF_HAND_1coord: 123..135
IPR011992EF-hand domain pairSUPERFAMILY47473EF-handcoord: 5..139

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Moc04g38930.1Moc04g38930.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0005509 calcium ion binding