Moc04g37960 (gene) Bitter gourd (OHB3-1) v2

Overview


Name	Moc04g37960
Type	gene
Organism	Momordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
Description	RNase H domain-containing protein
Location	chr4: 28431172 .. 28434074 (-)
RNA-Seq Expression	Moc04g37960
Synteny	Moc04g37960

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAACATCATGCTTTTGCTTGAAAACAAAACTGAAGCAAAGGCTAGACGTACGAAACTCACCGGAAGATGGATAAGAAATCGTCGGAAACTTCTCGCCGACGGCGAATCCCGGTTCAAAGGCCTCACCACAGAACGGTTAGATGATTCGAGATTTGAATAGAAGTGGAGAATCAGAAAATAGTGAGCACGCTGCACTGTGAATGTGAATACCGCGAGTGACGACTAAATGGACGGAAGACGAGCGGTAAATGTTTGAGAAATTACCATTTACTCCTCCAATTGTGAGTATGGAATAGGGATTAGATTTTAAACTAATTGATTGGATTTTTGTGTGATTTAAAAAGCTAATCTAATTTTGCATTTTTTTAAAATTATATTATTTATGAAATTTATAACTTTTTCAAACCCAAGATCCTTTTATGAAATTAAAAAAAAAAGTTTTCAAAATAAAACTTCCAAATCCATAACCCTTTTAAATTTAATAAATAAATAAATTCATCTATAAAACTCTAATGCAGACAAGGAATAATAACTTTTCAATATTATTAATGCGTTCCAAATTTGTATAATGTCCCTCGTGATGCTCATAATCATTGATTTAAATTTTATTATTGAATTGTGAATTAATAAAACTTGAGATTTTATATAAAAAGTTATGGTAAATTGCAAAAACCATCCCTAAATTATAATAGTTGTTGCAATTACACCCACAAACTTCTAATCATCAAAATTAAACCCTCCAACTTAAATAATTGTTTGAATTAATCTTTCGTGTTGGTTGGGTTAGAATTGTCGTTAAGTTCTGCATAAAAAACAAAAGGAAAAACAAAAATAAAACTCTCAAACTCAAGTTTGAAGAGCAGCGTCTGATCCTTTCTTGAAAACCTGATATGTGATGTTTGATTGCTTGCACAATTTCATCTTCCTTAAAACGCTTCCATTTTTAACTTTTTCTAGTTATTCATCTACGTAGTTTTGGGTTCCTCTTAGTCTCAATTTCGCCGAATTTTTTAAGATAATTTTGCAGAAAATGGAAGTCTGTCGCTGAGTTTGTAATTATCTCAAACAAAAGTCATCCTCTCTATACGACATTCTTTGAAGAAAATTAATGTTGTCGATACAACTAGAACTATGGAACTAGAACTATGGCTACTCCAAATGTTTGTGGTTGATGTATGAACATGGCAAGGCGAAAGCAGCAAGAAAACTCCTTGTTACACCAAACTCCCTCCCTCCAATCTCGTTAATCATCTAGAGGAAATGAAACTTAAGGTCTGGACTAAATTGTCGATTTTTTAAATTTAAGGATCAAATAGAAACTTATCATCAAACTTAGAGATCAAAAATGTATTTTAGAAATCTGTACGATACAAAAAAGAAATTGATTCCAAAAATAACTCACATGCTCGATATTAAAATAAGAGTATCATAATTATTTGTGGTACAATGAATAATCATATGATTATATTCTGTACCTGTCATGCTTTCTAAACAAAATAATCAAATACTTTTGATTTCTATAAAACAAAAAATAAATGAAGGATGATAATGAGAATAACAGTTTATTGCAATTATGAAGTCAAAAAAAAAAAAAACAGTTTATTGATGATAATATTTGTCCAAAGTTAAATGTTGTCTTATATTTTAGCTATCTATCTTCCATGTTTTTTTTTAAGTTAAACTTGAATATTTATATTCCAAGTTTTTTTTTTTATAATCATTTTATTTAAGGAAGAAGCATAATAATTTTTATATATTGGATGACTAAATATATGGTTGATAGTACAACAACACATAATAGATGGATGTGGCATTTTACGAAGAATGGCTCCTATACAGTAAGAAGTGGGTACAAGTTAGCTATGAGATTGAATCTACCTTCCCAATCCTCTAGCTCTGACAAAAATGAGAGGTGGTGGAAGGATTTCTGGAAAATGGACCTTCCCCCAAAAATTAAAATTTTTATGTGGAAAGCAATCAAAAATTGTATCCCAGTAATGACAAACTTGAAGAGAAGAGGTACGGAGGTGTCACCTATGTGTCCAATTTGCCTGAAAAAGGAGGAGACAGTGGATCACGCTCTGGTGGGTTGTAAACGGGCGTCTAAAATCTGGTCTTTGCTTCTGCCAGCATTAGATTCTTCTAATAATTTTAACGGTTGTTTTGTCGACCGGTGGACTAAGGTGATGTCGGATCTTTCGCAAAAAGAAGTCACCCTCGTTGGTATTACTAGCTGGACCCTGTGGAATGATCGAAATTCACACATTTTTAAAAATGGTACACCTGATGTTCATTTGAAATATAGATGGATTTTGGAGTATGCTGATGAATTTCTGAATAAAAATAAATTGACCTTGGACGCAAGCGGCCCCCTCCTGCAATCCCATCGTCTGGTGTCCCCCTCCAAAGGGGTTTTTAAAAATCAATACTGATGCTGCGTGTACCACCTCTGTTGGAAAGTCCGGAATAGGGATTTTAATCCGTGATGATGAAAATCGGGTACAAGCGGCGATGATTTCTTCCCAGCGTTTGGGAAGCCCCCTTTTGGTGGAAACCCTTGCTATCAGAGAAGGAATTCGTTTGGCGATGCGGATGGGTTTCAGTAAGGTGGTGGTCGAATCTGACTCCCTCGAAGCGGTTAAACTTATAGAGAAGAATGAGGGGTGGTTAAATGAGGTGGTTTCATTGATTGTTGACATCAGACATTTGTGTGTGAATTTTGACGATTTTCGAATTCAGCATGTTCGTCGTGAGGGAAATAGGGTCGCTCATATTCTCGCTAGAAATGCCATTTCTATAAATAGTCCCCTTCTTTGGCTTTGTGATTTTCCAGTTTGGCTTCTGAGCAGTGTGAAAGCTGATGATCGCACTAATATAGCCTTGGCAAGTTATTTTCTTTAA

mRNA sequence

ATGAACATCATGCTTTTGCTTGAAAACAAAACTGAAGCAAAGGCTAGACGTACGAAACTCACCGGAAGATGGATAAGAAATCGTCGGAAACTTCTCGCCGACGGCGAATCCCGGTTCAAAGGCCTCACCACAGAACGTAATGACAAACTTGAAGAGAAGAGGTACGGAGGTGTCACCTATGTGTCCAATTTGCCTGAAAAAGGAGGAGACAGTGGATCACGCTCTGGGATTTTAATCCGTGATGATGAAAATCGGGTACAAGCGGCGATGATTTCTTCCCAGCGTTTGGGAAGCCCCCTTTTGGTGGAAACCCTTGCTATCAGAGAAGGAATTCGTTTGGCGATGCGGATGGGTTTCAGTAAGGTGGTGGTCGAATCTGACTCCCTCGAAGCGGTTAAACTTATAGAGAAGAATGAGGGGTGGTTAAATGAGGTGGTTTCATTGATTGTTGACATCAGACATTTGTGTGTGAATTTTGACGATTTTCGAATTCAGCATGTTCGTCGTGAGGGAAATAGGGTCGCTCATATTCTCGCTAGAAATGCCATTTCTATAAATAGTCCCCTTCTTTGGCTTTGTGATTTTCCAGTTTGGCTTCTGAGCAGTGTGAAAGCTGATGATCGCACTAATATAGCCTTGGCAAGTTATTTTCTTTAA

Coding sequence (CDS)

ATGAACATCATGCTTTTGCTTGAAAACAAAACTGAAGCAAAGGCTAGACGTACGAAACTCACCGGAAGATGGATAAGAAATCGTCGGAAACTTCTCGCCGACGGCGAATCCCGGTTCAAAGGCCTCACCACAGAACGTAATGACAAACTTGAAGAGAAGAGGTACGGAGGTGTCACCTATGTGTCCAATTTGCCTGAAAAAGGAGGAGACAGTGGATCACGCTCTGGGATTTTAATCCGTGATGATGAAAATCGGGTACAAGCGGCGATGATTTCTTCCCAGCGTTTGGGAAGCCCCCTTTTGGTGGAAACCCTTGCTATCAGAGAAGGAATTCGTTTGGCGATGCGGATGGGTTTCAGTAAGGTGGTGGTCGAATCTGACTCCCTCGAAGCGGTTAAACTTATAGAGAAGAATGAGGGGTGGTTAAATGAGGTGGTTTCATTGATTGTTGACATCAGACATTTGTGTGTGAATTTTGACGATTTTCGAATTCAGCATGTTCGTCGTGAGGGAAATAGGGTCGCTCATATTCTCGCTAGAAATGCCATTTCTATAAATAGTCCCCTTCTTTGGCTTTGTGATTTTCCAGTTTGGCTTCTGAGCAGTGTGAAAGCTGATGATCGCACTAATATAGCCTTGGCAAGTTATTTTCTTTAA

Protein sequence

MNIMLLLENKTEAKARRTKLTGRWIRNRRKLLADGESRFKGLTTERNDKLEEKRYGGVTYVSNLPEKGGDSGSRSGILIRDDENRVQAAMISSQRLGSPLLVETLAIREGIRLAMRMGFSKVVVESDSLEAVKLIEKNEGWLNEVVSLIVDIRHLCVNFDDFRIQHVRREGNRVAHILARNAISINSPLLWLCDFPVWLLSSVKADDRTNIALASYFL

Homology

[Close]

BLAST of Moc04g37960 vs. NCBI nr
Match: XP_022158489.1 (uncharacterized protein LOC111024968 [Momordica charantia])

HSP 1 Score: 120.9 bits (302), Expect = 1.3e-23
Identity = 65/161 (40.37%), Postives = 92/161 (57.14%), Query Frame = 0


Query: 56  GGVTYVSNLPEKGGDSGSRSGILIRDDENRVQAAMISSQRLGSPLLVETLAIREGIRLAM 115
           GGV  + N      + GS  G+L+R+    +  AM+    + +PLL + LAIREG+ LA 
Sbjct: 72  GGVVKM-NTDAAVSEEGSGVGVLLREGNAEIVGAMVDFHMVKTPLLAKILAIREGLSLAT 131

Query: 116 RMGFSKVVVESDSLEAVKLIEKNEGWLNEVVSLIVDIRHLCVNFDDFRIQHVRREGNRVA 175
           R+G  +VVVE+DSLEA+ LI     W  E VS + DIR    +F +   QHV RE N VA
Sbjct: 132 RLGVHRVVVETDSLEALNLIGDKSPWKGEAVSWVEDIRAFARDFQEICFQHVFRESNAVA 191

Query: 176 HILARNAISINSPLLWLCDFPVWLLSSVKADDRTNIALASY 217
           + L R  IS+    LW  DFP+WL    +A  + ++AL ++
Sbjct: 192 YHLGRETISLRCGFLWRLDFPIWLGRLAEAGKQNSVALMAF 231

[Close]

BLAST of Moc04g37960 vs. NCBI nr
Match: XP_018855783.2 (uncharacterized protein LOC109018046 [Juglans regia] >KAF5469163.1 hypothetical protein F2P56_013257 [Juglans regia])

HSP 1 Score: 91.3 bits (225), Expect = 1.1e-14
Identity = 50/137 (36.50%), Postives = 84/137 (61.31%), Query Frame = 0


Query: 76  GILIRDD-ENRVQAAMISSQRLGSPLLVETLAIREGIRLAMRMGFSKVVVESDSLEAVKL 135
           G+++RD+ EN + A   S   L  P LVE LA+  G++  + +G SKVV+ESD L  V+ 
Sbjct: 230 GVVLRDEKENVLMAVSKSEHELHEPELVELLALLRGLQFNLHLGISKVVLESDCLLMVEA 289

Query: 136 IEKNEGWLNEVVSLIVDIRHLCVNFDDFRIQHVRREGNRVAHILARNAISINSPLLWLCD 195
           +  NE  L+   +L+ ++R+L  +F+ +++Q V R GN+VAH LA++A S+    +W+  
Sbjct: 290 LATNEDLLSSQSNLLKEVRNLLSHFEQYQVQRVIRGGNQVAHFLAKHAWSVYDLKMWMGS 349

Query: 196 FPVWLLSSVKADDRTNI 212
            P +L   +   D+TN+
Sbjct: 350 VPSFLHQYIWL-DQTNL 365

[Close]

BLAST of Moc04g37960 vs. NCBI nr
Match: XP_027067558.1 (uncharacterized protein LOC113693185 [Coffea arabica])

HSP 1 Score: 89.0 bits (219), Expect = 5.6e-14
Identity = 49/146 (33.56%), Postives = 81/146 (55.48%), Query Frame = 0


Query: 71  SGSRSGILIRD-DENRVQAAMISSQRLGSPLLVETLAIREGIRLAMRMGFSKVVVESDSL 130
           +G+  G++ RD D N VQA  I     G   + E  A+R+G+ +A   G+ ++ +++D  
Sbjct: 14  AGAELGMIARDSDGNIVQARGIRKYSSGGAEMEEADALRQGLLMAREAGWRRIEMQTDCK 73

Query: 131 EAVKLIEKNEGWLNEVVSLIVDIRHLCVNFDDFRIQHVRREGNRVAHILARNAISINSPL 190
            A++ I K       + ++I DIRHL   F D     V R+GNR AH +A+ A  + S +
Sbjct: 74  AAIETICKTGLGETPIGTIIEDIRHLSDMFQDCTFSFVYRDGNRNAHKMAQFATKLVSKI 133

Query: 191 LWLCDFPVWLLSSVKADDRTNIALAS 216
           +W   FP+W   S++ D+RTN+ L +
Sbjct: 134 IWKQSFPLWFKESIQEDNRTNVLLCN 159

[Close]

BLAST of Moc04g37960 vs. NCBI nr
Match: XP_030939190.1 (uncharacterized protein LOC115964041 [Quercus lobata])

HSP 1 Score: 86.7 bits (213), Expect = 2.8e-13
Identity = 52/132 (39.39%), Postives = 76/132 (57.58%), Query Frame = 0


Query: 76  GILIRDDENRVQAAMISSQRLGSPLL-VETLAIREGIRLAMRMGFSKVVVESDSLEAVKL 135
           G++IR+ +  V A++     L + ++ VETLA R  +  A+ +GF K+V+E DS   +KL
Sbjct: 848 GVVIRNHDGLVMASLSDVVPLPAIVIEVETLAARRAVEFALELGFEKIVLEGDSDVLIKL 907

Query: 136 IEKNEGWLNEVVSLIVDIRHLCVNFDDFRIQHVRREGNRVAHILARNAISINSPLLWLCD 195
           +      L     LI DI  L   F  FR  HVRR  N+VA++LAR AIS +   +W+ D
Sbjct: 908 LNSCSRSLAPFGHLINDIMFLASRFTCFRATHVRRHCNKVAYLLARKAISFSPLSVWMED 967

Query: 196 FPVWLLSSVKAD 207
            P  LLS ++AD
Sbjct: 968 VPPDLLSVIQAD 979

[Close]

BLAST of Moc04g37960 vs. NCBI nr
Match: XP_023919081.1 (uncharacterized protein LOC112030645 [Quercus suber])

HSP 1 Score: 86.7 bits (213), Expect = 2.8e-13
Identity = 51/143 (35.66%), Postives = 82/143 (57.34%), Query Frame = 0


Query: 65  PEKGGDSGSRSGILIRDDENRVQAAMISSQRLG-SPLLVETLAIREGIRLAMRMGFSKVV 124
           PE+   +G   G++IR+ E  V  ++     L  SPL +E  AI   +  A  +GF + +
Sbjct: 693 PEQENSAG--VGVVIRNSEGLVLGSLSKLVPLAYSPLEIEATAIATALEFASDLGFQRAI 752

Query: 125 VESDSLEAVKLIEKNEGWLNEVVSLIVDIRHLCVNFDDFRIQHVRREGNRVAHILARNAI 184
           +E+DSL  VK + ++  +L+ V  ++ +IR     F++    HV+REGN VAH LAR+AI
Sbjct: 753 IETDSLVLVKALREDSEFLSAVGLILDEIRRKVNFFNELHYSHVKREGNIVAHKLARHAI 812

Query: 185 SINSPLLWLCDFPVWLLSSVKAD 207
           S++  ++W+ D P  L   V AD
Sbjct: 813 SVSDIVVWMEDVPPLLFPVVLAD 833

[Close]

BLAST of Moc04g37960 vs. ExPASy TrEMBL
Match: A0A6J1DZK3 (uncharacterized protein LOC111024968 OS=Momordica charantia OX=3673 GN=LOC111024968 PE=4 SV=1)

HSP 1 Score: 120.9 bits (302), Expect = 6.4e-24
Identity = 65/161 (40.37%), Postives = 92/161 (57.14%), Query Frame = 0


Query: 56  GGVTYVSNLPEKGGDSGSRSGILIRDDENRVQAAMISSQRLGSPLLVETLAIREGIRLAM 115
           GGV  + N      + GS  G+L+R+    +  AM+    + +PLL + LAIREG+ LA 
Sbjct: 72  GGVVKM-NTDAAVSEEGSGVGVLLREGNAEIVGAMVDFHMVKTPLLAKILAIREGLSLAT 131

Query: 116 RMGFSKVVVESDSLEAVKLIEKNEGWLNEVVSLIVDIRHLCVNFDDFRIQHVRREGNRVA 175
           R+G  +VVVE+DSLEA+ LI     W  E VS + DIR    +F +   QHV RE N VA
Sbjct: 132 RLGVHRVVVETDSLEALNLIGDKSPWKGEAVSWVEDIRAFARDFQEICFQHVFRESNAVA 191

Query: 176 HILARNAISINSPLLWLCDFPVWLLSSVKADDRTNIALASY 217
           + L R  IS+    LW  DFP+WL    +A  + ++AL ++
Sbjct: 192 YHLGRETISLRCGFLWRLDFPIWLGRLAEAGKQNSVALMAF 231

[Close]

BLAST of Moc04g37960 vs. ExPASy TrEMBL
Match: A0A2I4HHZ2 (uncharacterized protein LOC109018046 OS=Juglans regia OX=51240 GN=LOC109018046 PE=4 SV=2)

HSP 1 Score: 91.3 bits (225), Expect = 5.5e-15
Identity = 50/137 (36.50%), Postives = 84/137 (61.31%), Query Frame = 0


Query: 76  GILIRDD-ENRVQAAMISSQRLGSPLLVETLAIREGIRLAMRMGFSKVVVESDSLEAVKL 135
           G+++RD+ EN + A   S   L  P LVE LA+  G++  + +G SKVV+ESD L  V+ 
Sbjct: 230 GVVLRDEKENVLMAVSKSEHELHEPELVELLALLRGLQFNLHLGISKVVLESDCLLMVEA 289

Query: 136 IEKNEGWLNEVVSLIVDIRHLCVNFDDFRIQHVRREGNRVAHILARNAISINSPLLWLCD 195
           +  NE  L+   +L+ ++R+L  +F+ +++Q V R GN+VAH LA++A S+    +W+  
Sbjct: 290 LATNEDLLSSQSNLLKEVRNLLSHFEQYQVQRVIRGGNQVAHFLAKHAWSVYDLKMWMGS 349

Query: 196 FPVWLLSSVKADDRTNI 212
            P +L   +   D+TN+
Sbjct: 350 VPSFLHQYIWL-DQTNL 365

[Close]

BLAST of Moc04g37960 vs. ExPASy TrEMBL
Match: A0A6P6SNV4 (uncharacterized protein LOC113693185 OS=Coffea arabica OX=13443 GN=LOC113693185 PE=4 SV=1)

HSP 1 Score: 89.0 bits (219), Expect = 2.7e-14
Identity = 49/146 (33.56%), Postives = 81/146 (55.48%), Query Frame = 0


Query: 71  SGSRSGILIRD-DENRVQAAMISSQRLGSPLLVETLAIREGIRLAMRMGFSKVVVESDSL 130
           +G+  G++ RD D N VQA  I     G   + E  A+R+G+ +A   G+ ++ +++D  
Sbjct: 14  AGAELGMIARDSDGNIVQARGIRKYSSGGAEMEEADALRQGLLMAREAGWRRIEMQTDCK 73

Query: 131 EAVKLIEKNEGWLNEVVSLIVDIRHLCVNFDDFRIQHVRREGNRVAHILARNAISINSPL 190
            A++ I K       + ++I DIRHL   F D     V R+GNR AH +A+ A  + S +
Sbjct: 74  AAIETICKTGLGETPIGTIIEDIRHLSDMFQDCTFSFVYRDGNRNAHKMAQFATKLVSKI 133

Query: 191 LWLCDFPVWLLSSVKADDRTNIALAS 216
           +W   FP+W   S++ D+RTN+ L +
Sbjct: 134 IWKQSFPLWFKESIQEDNRTNVLLCN 159

[Close]

BLAST of Moc04g37960 vs. ExPASy TrEMBL
Match: A0A6P6UIH5 (uncharacterized protein LOC113710661 OS=Coffea arabica OX=13443 GN=LOC113710661 PE=4 SV=1)

HSP 1 Score: 86.3 bits (212), Expect = 1.8e-13
Identity = 49/146 (33.56%), Postives = 81/146 (55.48%), Query Frame = 0


Query: 71   SGSRSGILIRDDE-NRVQAAMISSQRLGSPLLVETLAIREGIRLAMRMGFSKVVVESDSL 130
            +G+  G++ RD E N VQA  I         + E  A+R+G+ +A   G+ ++ +++D  
Sbjct: 1013 AGAGLGMIARDCEGNIVQARGIRKYSSAGAEMEEADALRQGLLMAREAGWRRIEMQTDCK 1072

Query: 131  EAVKLIEKNEGWLNEVVSLIVDIRHLCVNFDDFRIQHVRREGNRVAHILARNAISINSPL 190
             A++ I K       + ++I DIRHL   F D     V R+GNR AH +A+ A  + S +
Sbjct: 1073 AAIETICKTGLGETPIGTIIEDIRHLSDLFQDCTFSFVYRDGNRSAHKMAQFATKLVSKV 1132

Query: 191  LWLCDFPVWLLSSVKADDRTNIALAS 216
            +W   FP+WL  S++ D+RTN+ L +
Sbjct: 1133 IWKQSFPLWLKESIQEDNRTNVPLCN 1158

[Close]

BLAST of Moc04g37960 vs. ExPASy TrEMBL
Match: A0A1D6MWR3 (RNase H domain-containing protein OS=Zea mays OX=4577 GN=ZEAMMB73_Zm00001d041519 PE=4 SV=1)

HSP 1 Score: 83.6 bits (205), Expect = 1.1e-12
Identity = 44/132 (33.33%), Postives = 69/132 (52.27%), Query Frame = 0


Query: 76  GILIRDDENRVQAAMIS-SQRLGSPLLVETLAIREGIRLAMRMGFSKVVVESDSLEAVKL 135
           G+++R+ +  V AA     + +   L  E LA R+G+ LA  +   K ++E D+   V L
Sbjct: 83  GVVMRNSQGEVLAAATQLYEHIPDALTSEALAARDGVVLAQMLSVEKAILEVDNSVLVAL 142

Query: 136 IEKNEGWLNEVVSLIVDIRHLCVNFDDFRIQHVRREGNRVAHILARNAISINSPLLWLCD 195
           +   EG  + +  L  +I+   +NF  F++  V REGN VAH+ A  AI +   L WL  
Sbjct: 143 LRSEEGRRSAIAGLWQEIKEKSMNFRSFQVSFVNREGNEVAHLCASKAIELAPVLFWLDC 202

Query: 196 FPVWLLSSVKAD 207
           FP WL+ +   D
Sbjct: 203 FPSWLMEAASRD 214

[Close]

BLAST of Moc04g37960 vs. TAIR 10
Match: AT5G42965.1 (Polynucleotidyl transferase, ribonuclease H-like superfamily protein )

HSP 1 Score: 57.4 bits (137), Expect = 1.7e-08
Identity = 32/91 (35.16%), Postives = 52/91 (57.14%), Query Frame = 0


Query: 95  RLGSPLLVETLAIREGIRLAMRMGFSKVVVESDSLEAVKLIEKNEGWLNEVVSLIVDIRH 154
           R+ + L VE  A+R       R  + KV+ ESDS   V L+  +  + N +   I DIRH
Sbjct: 11  RVQTVLEVELEAMRWAALSMSRFNYKKVIFESDSQHLVSLLGDDTDYPN-LAPRIQDIRH 70

Query: 155 LCVNFDDFRIQHVRREGNRVAHILARNAISI 186
           +   F++ ++ H++REGN VA  +AR ++S+
Sbjct: 71  IFQQFEEVKVMHIKREGNSVADRIARESLSL 100

[Close]

BLAST of Moc04g37960 vs. TAIR 10
Match: AT2G13980.1 (Polynucleotidyl transferase, ribonuclease H-like superfamily protein )

HSP 1 Score: 51.2 bits (121), Expect = 1.2e-06
Identity = 32/104 (30.77%), Postives = 52/104 (50.00%), Query Frame = 0


Query: 98  SPLLVETLAIREGIRLAMRMGFSKVVVESDSLEAVKLIEKNEGWLNEVVSLIVDIRHLCV 157
           +PL  ET A+   ++     G+ +V++E D      L+  +    N + +L+ DIR    
Sbjct: 62  TPLEAETKALLAALQQTWIRGYLRVIMEGDCETLTNLVSGSSSH-NHLANLLDDIRIWAK 121

Query: 158 NFDDFRIQHVRREGNRVAHILARNAISINSPLLW--LCDFPVWL 200
            F + +   VRR GN+VAH LA+  +  NS   +   C  P+WL
Sbjct: 122 KFSNVQFSFVRRGGNKVAHELAK--LGCNSTCFYSDSCTLPIWL 162

[Close]

BLAST of Moc04g37960 vs. TAIR 10
Match: AT2G34320.1 (Polynucleotidyl transferase, ribonuclease H-like superfamily protein )

HSP 1 Score: 47.0 bits (110), Expect = 2.3e-05
Identity = 31/111 (27.93%), Postives = 58/111 (52.25%), Query Frame = 0


Query: 95  RLGSPLLVETLAIREGIRLAMRMGFSKVVVESDSLEAVKLIEKNEGWLNEVVSLIVDIRH 154
           R  + L  E  A+R  +    R  + +++ ESD+   V L+  ++ W   +   + DI+ 
Sbjct: 183 RTKNVLEAELEALRWAVLTMSRFNYKRIIFESDAQALVNLLNSDDFW-PTLQPALEDIQQ 242

Query: 155 LCVNFDDFRIQHVRREGNRVAHILARNAISINS--PLLWLCDFPVWLLSSV 204
           L  +F++ + +   R GN+VA  +AR +IS ++  P L+    P WL S++
Sbjct: 243 LLHHFEEVKFEFTPRGGNKVADRIARESISFSNYDPKLFSI-VPQWLRSTL 291

[Close]

BLAST of Moc04g37960 vs. TAIR 10
Match: AT1G10000.1 (Ribonuclease H-like superfamily protein )

HSP 1 Score: 45.4 bits (106), Expect = 6.6e-05
Identity = 30/104 (28.85%), Postives = 50/104 (48.08%), Query Frame = 0


Query: 83  ENRVQAAMISSQRLGSPLLVETLAIREGIRLAMRMGFSKVVVESDSLEAVKLIEKNEGWL 142
           E  +       +R  SPL  E  AI+  +  A+++  S ++V SDS   V  +  N   L
Sbjct: 188 EKEITTFSAGCRRFPSPLAAEAWAIKSAMLHALQLERSDLLVLSDSKSIVDALNSNVS-L 247

Query: 143 NEVVSLIVDIRHLCVNFDDFRIQHVRREGNRVAHILARNAISIN 187
           NE+  L+V+IR +   F     Q + R  N +A   A+ ++ I+
Sbjct: 248 NEIFGLLVEIRSIRNRFRSISFQFIPRLVNSIADAAAKLSLCIS 290

[Close]

BLAST of Moc04g37960 vs. TAIR 10
Match: AT4G29090.1 (Ribonuclease H-like superfamily protein )

HSP 1 Score: 44.7 bits (104), Expect = 1.1e-04
Identity = 34/130 (26.15%), Postives = 64/130 (49.23%), Query Frame = 0


Query: 76  GILIRDDENRVQ-AAMISSQRLGSPLLVETLAIREGIRLAMRMGFSKVVVESDSLEAVKL 135
           G ++R+++  V+     +  +L S L  E  A+R  +    R  ++ V+ ESDS   +++
Sbjct: 446 GWVLRNEKGEVKWMGARALPKLKSVLEAELEAMRWAVLSLSRFQYNYVIFESDSQVLIEI 505

Query: 136 IEKNEGWLNEVVSLIVDIRHLCVNFDDFRIQHVRREGNRVAHILARNAIS-INSPLLWLC 195
           +  +E W   +   I D++ L   F + +   + REGN +A  +AR ++S +N       
Sbjct: 506 LNNDEIW-PSLKPTIQDLQRLLSQFTEVKFVFIPREGNTLAERVARESLSFLNYDPKLYS 565

Query: 196 DFPVWLLSSV 204
             P W  SS+
Sbjct: 566 IVPSWARSSM 574

The following BLAST results are available for this feature:

BLAST of Moc04g37960 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022158489.1	1.3e-23	40.37	uncharacterized protein LOC111024968 [Momordica charantia]	[more]
XP_018855783.2	1.1e-14	36.50	uncharacterized protein LOC109018046 [Juglans regia] >KAF5469163.1 hypothetical ...	[more]
XP_027067558.1	5.6e-14	33.56	uncharacterized protein LOC113693185 [Coffea arabica]	[more]
XP_030939190.1	2.8e-13	39.39	uncharacterized protein LOC115964041 [Quercus lobata]	[more]
XP_023919081.1	2.8e-13	35.66	uncharacterized protein LOC112030645 [Quercus suber]	[more]

BLAST of Moc04g37960 vs. NCBI nr


Match Name	E-value	Identity	Description

BLAST of Moc04g37960 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1DZK3	6.4e-24	40.37	uncharacterized protein LOC111024968 OS=Momordica charantia OX=3673 GN=LOC111024...	[more]
A0A2I4HHZ2	5.5e-15	36.50	uncharacterized protein LOC109018046 OS=Juglans regia OX=51240 GN=LOC109018046 P...	[more]
A0A6P6SNV4	2.7e-14	33.56	uncharacterized protein LOC113693185 OS=Coffea arabica OX=13443 GN=LOC113693185 ...	[more]
A0A6P6UIH5	1.8e-13	33.56	uncharacterized protein LOC113710661 OS=Coffea arabica OX=13443 GN=LOC113710661 ...	[more]
A0A1D6MWR3	1.1e-12	33.33	RNase H domain-containing protein OS=Zea mays OX=4577 GN=ZEAMMB73_Zm00001d041519...	[more]

BLAST of Moc04g37960 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G42965.1	1.7e-08	35.16	Polynucleotidyl transferase, ribonuclease H-like superfamily protein	[more]
AT2G13980.1	1.2e-06	30.77	Polynucleotidyl transferase, ribonuclease H-like superfamily protein	[more]
AT2G34320.1	2.3e-05	27.93	Polynucleotidyl transferase, ribonuclease H-like superfamily protein	[more]
AT1G10000.1	6.6e-05	28.85	Ribonuclease H-like superfamily protein	[more]
AT4G29090.1	1.1e-04	26.15	Ribonuclease H-like superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (OHB3-1) v2
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002156	Ribonuclease H domain	PFAM	PF13456	RVT_3	coord: 70..182 e-value: 2.2E-24 score: 85.6
IPR002156	Ribonuclease H domain	PROSITE	PS50879	RNASE_H	coord: 25..184 score: 8.853633
IPR036397	Ribonuclease H superfamily	GENE3D	3.30.420.10		coord: 62..184 e-value: 1.4E-15 score: 59.5
None	No IPR available	PANTHER	PTHR47723	OS05G0353850 PROTEIN	coord: 73..202
IPR044730	Ribonuclease H-like domain, plant type	CDD	cd06222	RNase_H_like	coord: 67..180 e-value: 2.65546E-28 score: 101.622
IPR012337	Ribonuclease H-like superfamily	SUPERFAMILY	53098	Ribonuclease H-like	coord: 74..191

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Moc04g37960.1	Moc04g37960.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0003676	nucleic acid binding
molecular_function	GO:0004523	RNA-DNA hybrid ribonuclease activity