Moc04g37340 (gene) Bitter gourd (OHB3-1) v2

Overview
NameMoc04g37340
Typegene
OrganismMomordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
DescriptionTryptophan synthase
Locationchr4: 27969446 .. 27972360 (+)
RNA-Seq ExpressionMoc04g37340
SyntenyMoc04g37340
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCCTCTCACTTCCTTGTTGCTGCATCGTCGACTTCTCCAGCCATCAATGGCAGAGGCACTTCATTTTCCCCTTCTTTTTCTGTGTTTATATCTTCCTTTATCTTGGTTTCCAACTTCACCTGCTATGAAATGAAGCTGTAAATGGATCGAATCGTGAACCCTTTTCATTTTACTTCTTGTGGACGTTGATCGCGCTTTGTTTAGTCATTTCTCTACTTCTTTGTGTCTTTTAGTTCTGACTCAGATTGTGGAATCTTGTTTTACCTTCAAAATGACCTTTGCTTTTGGTTAAAGTTTGGAGATGAAATTGCAGAATCTGTGGGGATTTTTGGTTTGGACATAATCTAGTTGTTTTTTTTTTCCTCTTTTGGCCTTGAGGAAATGAAAGATGGTGGACTGTAAAACCAACATCAGCTGTAAAATTAAAGGCAAGAAACAGTATCTGTAAGAGAGAGAGAGATTTCACTGTTGAAGTGGCAGATTTCACTGTTGAAGTGGCATTTGTCTGGTTTATTGCTTGATGATATGCTGCTGTGTTTTGGAAGCTTTTTGCTTGCTTTAGTCAGACATGTGATGATTCTGAATCCACTTTTGCCTTTTCTTTTATTTTTCACAGTAAACTGCATTTTCCTGCTTTGCTTTCCAAACTTAATAGAGTAAATCATTATCGTGGGACTTCGCTTCTAAGGCATTCTGTAATTATCGGTCAGGTGTTATTCTTTGGATTGGAGTTCCTTTATCTGGAGGACCTCCTTTTGTCGGACTGTCATTTTATACGCCTTTGTATATCTTTTCATTTCTCTCGATGAAAGCCTAAGTTTCTGGTAAATTTTTTATTTTTTTTTAAAGGAGAGTAAATCATCTTAGCTATAGAGAACATTTAATGAAAATGCTGTGATGATTCCCCAATGGATACTTATATCTTTACACCACCCTCCCTCCCTCTCTGCCCCGTTCCCCACATTTCTGACCATTTAAACTACTTCACCTCAGTTGGGGAGGAACTGCCTGGACGCCTTTCACTAAAGAGAAATCCAAGAAATTTGAGGTTCTCAAATGGTTATAGAACAAGAGCAGCATCGAATCGTAATTTAAAGCCAGTCGAGGTCCCGCGTCGATGGTACAATCTGGTTGCAGATCTTCCTGTAAAACCTCCTCCTCCGTTGCATCCCAAGACTTTCCAACCAATCAAACCCCAAGATTTATCACCTCTCTTTCCTGATGCATTGATTGAGCAAGAGGCCAGTACTGATCGGTACATAAACATCCCCGACGAAGTTGTTGATATCTACGGGCTTTGGCGCCCGACTCCTCTTATCAGGTTTGTGGTATGGTATGTCCTTAAAATGTATGCAAGCTTATTTGCCATAATGTTTTCTGTATGACATCTTGAATCCTTTCCCTGCTAAGTTTTCCATACTTGCATTTTTCATTGCTCTAGAGTATCTAACAGCGTTTTCGATCTATGTTAGAGCCAAGAGGTTAGAGAAGCTTCTTGACACGCCAGCCCGAATTTACTACAAGTATGAAGGAGTCAGCCCTGCCGGATCACATAAAGCGAACTCTGCTGTTCCACAGGTTTGGTATAATGTACAGGAAGGTGTCAAGAATGTTGTTACAGAAACTGGGGCTGGTCAATGGGGAAGCGCTTTGGCCTTTGCTTGTAGCATATTCGGTCTCGGTTGTGAGGTAAGGGGAAATAGTTGTGATTGATATGTAGTATAAAATCGAAGTTACGATCAACTATCGATTTAGATGTCGAGAAAACTGTTCAAAACTTATACTTCTTGGTTGCTTCTGAAACATATCACAGTGAATTTGTCTTGTTTTGTTAAATAGGTGTGGCAAGTGCGTGCTTCTTATGATCAAAAACCGTATCGACGACTGATGATGCAAACTTGGGGGGTGAAAGTCCACCCATCTCCTTCAGATCTTACAGATGCAGGTAGAAAATTGCTTCAAGTCGACCCCTCGAGCCCGGGAAGTTTAGGAATTGCCATTTCAGAAGCTGTGGAGGTTGCAGCTCTGAATGCTGATACCAAGTACTGTTTGGGAAGTGTCCTCAACCATGTTTTGCTTCACCAAACCGTCATTGGTGAAGAGTGTCTAAAACAAATGGAAGCTTTAGGCGAGAGCCCGGATGTCATCATAGGATGCACCGGTGGTGGATCAAATTTTGCTGGTCTTAGCTTCCCGTTCCTCCGCGAGAAACTTGCTGGGAAAATAAACCCCATTCTTAGAGCAGTTGAACCCGCAGCCTGTCCTTCGTTAACAAAAGGCGTCTACGCTTACGATTTTGGCGATACAGCCGGGATGACTCCATTGATGAAGATGCACACCCTTGGTCACAACTTCATTCCTGACCCAATTCATGCTGGTAAGTGGTAACACATAATACGCTATCGAAACAGATATAATGCATCAAACTCTTTGTTTCTCTTGATCATCGTCTCGGCTCAACACTTTTTCGCAGGTGGATTACGGTATCATGGTATGGCTCCATTGATCTCACATGTCTATAATCTCGGTCTTTTGGAAGCTATATCACTTCCCCAGACAGAGTGCTTTCAAGGTACCAGAATTTATTCAAAAGTTAAGCGAACCAACTACTGGAATTACAGCAACAGCAACAGCAACTGAGTTTCTCTTTGTTTGCAGGTGCAATACAGTTTGCTCGATCAGAAGGACTAATAGCAGCTCCCGAGCCAACTCACGCCATAGCTGCAACGATCCGAGAGGCTCTTCGGTGTCGGGAAACCGGAGAATCGAAAGTTATTCTCACAGCCATGTGCGGGCACGGTCACTTCGACTTGCCAGCTTATGAGAAGTACCTGCAAGGAGGGATGACAGATCTTTCTTTTGCAGAGGAGAAGATCCAAGAATCACTAACCACCATTCCTCAGGTGGCTTGA

mRNA sequence

ATGGCCTCTCACTTCCTTGTTGCTGCATCGTCGACTTCTCCAGCCATCAATGGCAGAGTTGGGGAGGAACTGCCTGGACGCCTTTCACTAAAGAGAAATCCAAGAAATTTGAGGTTCTCAAATGGTTATAGAACAAGAGCAGCATCGAATCGTAATTTAAAGCCAGTCGAGGTCCCGCGTCGATGGTACAATCTGGTTGCAGATCTTCCTGTAAAACCTCCTCCTCCGTTGCATCCCAAGACTTTCCAACCAATCAAACCCCAAGATTTATCACCTCTCTTTCCTGATGCATTGATTGAGCAAGAGGCCAGTACTGATCGGTACATAAACATCCCCGACGAAGTTGTTGATATCTACGGGCTTTGGCGCCCGACTCCTCTTATCAGAGCCAAGAGGTTAGAGAAGCTTCTTGACACGCCAGCCCGAATTTACTACAAGTATGAAGGAGTCAGCCCTGCCGGATCACATAAAGCGAACTCTGCTGTTCCACAGGTTTGGTATAATGTACAGGAAGGTGTCAAGAATGTTGTTACAGAAACTGGGGCTGGTCAATGGGGAAGCGCTTTGGCCTTTGCTTGTAGCATATTCGGTCTCGGTTGTGAGGTGTGGCAAGTGCGTGCTTCTTATGATCAAAAACCGTATCGACGACTGATGATGCAAACTTGGGGGGTGAAAGTCCACCCATCTCCTTCAGATCTTACAGATGCAGGTAGAAAATTGCTTCAAGTCGACCCCTCGAGCCCGGGAAGTTTAGGAATTGCCATTTCAGAAGCTGTGGAGGTTGCAGCTCTGAATGCTGATACCAAGTACTGTTTGGGAAGTGTCCTCAACCATGTTTTGCTTCACCAAACCGTCATTGGTGAAGAGTGTCTAAAACAAATGGAAGCTTTAGGCGAGAGCCCGGATGTCATCATAGGATGCACCGGTGGTGGATCAAATTTTGCTGGTCTTAGCTTCCCGTTCCTCCGCGAGAAACTTGCTGGGAAAATAAACCCCATTCTTAGAGCAGTTGAACCCGCAGCCTGTCCTTCGTTAACAAAAGGCGTCTACGCTTACGATTTTGGCGATACAGCCGGGATGACTCCATTGATGAAGATGCACACCCTTGGTCACAACTTCATTCCTGACCCAATTCATGCTGGTGGATTACGGTATCATGGTATGGCTCCATTGATCTCACATGTCTATAATCTCGGTCTTTTGGAAGCTATATCACTTCCCCAGACAGAGTGCTTTCAAGGTGCAATACAGTTTGCTCGATCAGAAGGACTAATAGCAGCTCCCGAGCCAACTCACGCCATAGCTGCAACGATCCGAGAGGCTCTTCGGTGTCGGGAAACCGGAGAATCGAAAGTTATTCTCACAGCCATGTGCGGGCACGGTCACTTCGACTTGCCAGCTTATGAGAAGTACCTGCAAGGAGGGATGACAGATCTTTCTTTTGCAGAGGAGAAGATCCAAGAATCACTAACCACCATTCCTCAGGTGGCTTGA

Coding sequence (CDS)

ATGGCCTCTCACTTCCTTGTTGCTGCATCGTCGACTTCTCCAGCCATCAATGGCAGAGTTGGGGAGGAACTGCCTGGACGCCTTTCACTAAAGAGAAATCCAAGAAATTTGAGGTTCTCAAATGGTTATAGAACAAGAGCAGCATCGAATCGTAATTTAAAGCCAGTCGAGGTCCCGCGTCGATGGTACAATCTGGTTGCAGATCTTCCTGTAAAACCTCCTCCTCCGTTGCATCCCAAGACTTTCCAACCAATCAAACCCCAAGATTTATCACCTCTCTTTCCTGATGCATTGATTGAGCAAGAGGCCAGTACTGATCGGTACATAAACATCCCCGACGAAGTTGTTGATATCTACGGGCTTTGGCGCCCGACTCCTCTTATCAGAGCCAAGAGGTTAGAGAAGCTTCTTGACACGCCAGCCCGAATTTACTACAAGTATGAAGGAGTCAGCCCTGCCGGATCACATAAAGCGAACTCTGCTGTTCCACAGGTTTGGTATAATGTACAGGAAGGTGTCAAGAATGTTGTTACAGAAACTGGGGCTGGTCAATGGGGAAGCGCTTTGGCCTTTGCTTGTAGCATATTCGGTCTCGGTTGTGAGGTGTGGCAAGTGCGTGCTTCTTATGATCAAAAACCGTATCGACGACTGATGATGCAAACTTGGGGGGTGAAAGTCCACCCATCTCCTTCAGATCTTACAGATGCAGGTAGAAAATTGCTTCAAGTCGACCCCTCGAGCCCGGGAAGTTTAGGAATTGCCATTTCAGAAGCTGTGGAGGTTGCAGCTCTGAATGCTGATACCAAGTACTGTTTGGGAAGTGTCCTCAACCATGTTTTGCTTCACCAAACCGTCATTGGTGAAGAGTGTCTAAAACAAATGGAAGCTTTAGGCGAGAGCCCGGATGTCATCATAGGATGCACCGGTGGTGGATCAAATTTTGCTGGTCTTAGCTTCCCGTTCCTCCGCGAGAAACTTGCTGGGAAAATAAACCCCATTCTTAGAGCAGTTGAACCCGCAGCCTGTCCTTCGTTAACAAAAGGCGTCTACGCTTACGATTTTGGCGATACAGCCGGGATGACTCCATTGATGAAGATGCACACCCTTGGTCACAACTTCATTCCTGACCCAATTCATGCTGGTGGATTACGGTATCATGGTATGGCTCCATTGATCTCACATGTCTATAATCTCGGTCTTTTGGAAGCTATATCACTTCCCCAGACAGAGTGCTTTCAAGGTGCAATACAGTTTGCTCGATCAGAAGGACTAATAGCAGCTCCCGAGCCAACTCACGCCATAGCTGCAACGATCCGAGAGGCTCTTCGGTGTCGGGAAACCGGAGAATCGAAAGTTATTCTCACAGCCATGTGCGGGCACGGTCACTTCGACTTGCCAGCTTATGAGAAGTACCTGCAAGGAGGGATGACAGATCTTTCTTTTGCAGAGGAGAAGATCCAAGAATCACTAACCACCATTCCTCAGGTGGCTTGA

Protein sequence

MASHFLVAASSTSPAINGRVGEELPGRLSLKRNPRNLRFSNGYRTRAASNRNLKPVEVPRRWYNLVADLPVKPPPPLHPKTFQPIKPQDLSPLFPDALIEQEASTDRYINIPDEVVDIYGLWRPTPLIRAKRLEKLLDTPARIYYKYEGVSPAGSHKANSAVPQVWYNVQEGVKNVVTETGAGQWGSALAFACSIFGLGCEVWQVRASYDQKPYRRLMMQTWGVKVHPSPSDLTDAGRKLLQVDPSSPGSLGIAISEAVEVAALNADTKYCLGSVLNHVLLHQTVIGEECLKQMEALGESPDVIIGCTGGGSNFAGLSFPFLREKLAGKINPILRAVEPAACPSLTKGVYAYDFGDTAGMTPLMKMHTLGHNFIPDPIHAGGLRYHGMAPLISHVYNLGLLEAISLPQTECFQGAIQFARSEGLIAAPEPTHAIAATIREALRCRETGESKVILTAMCGHGHFDLPAYEKYLQGGMTDLSFAEEKIQESLTTIPQVA
Homology
BLAST of Moc04g37340 vs. NCBI nr
Match: XP_022134316.1 (uncharacterized protein LOC111006607 [Momordica charantia])

HSP 1 Score: 1012.7 bits (2617), Expect = 1.1e-291
Identity = 497/497 (100.00%), Postives = 497/497 (100.00%), Query Frame = 0

Query: 1   MASHFLVAASSTSPAINGRVGEELPGRLSLKRNPRNLRFSNGYRTRAASNRNLKPVEVPR 60
           MASHFLVAASSTSPAINGRVGEELPGRLSLKRNPRNLRFSNGYRTRAASNRNLKPVEVPR
Sbjct: 1   MASHFLVAASSTSPAINGRVGEELPGRLSLKRNPRNLRFSNGYRTRAASNRNLKPVEVPR 60

Query: 61  RWYNLVADLPVKPPPPLHPKTFQPIKPQDLSPLFPDALIEQEASTDRYINIPDEVVDIYG 120
           RWYNLVADLPVKPPPPLHPKTFQPIKPQDLSPLFPDALIEQEASTDRYINIPDEVVDIYG
Sbjct: 61  RWYNLVADLPVKPPPPLHPKTFQPIKPQDLSPLFPDALIEQEASTDRYINIPDEVVDIYG 120

Query: 121 LWRPTPLIRAKRLEKLLDTPARIYYKYEGVSPAGSHKANSAVPQVWYNVQEGVKNVVTET 180
           LWRPTPLIRAKRLEKLLDTPARIYYKYEGVSPAGSHKANSAVPQVWYNVQEGVKNVVTET
Sbjct: 121 LWRPTPLIRAKRLEKLLDTPARIYYKYEGVSPAGSHKANSAVPQVWYNVQEGVKNVVTET 180

Query: 181 GAGQWGSALAFACSIFGLGCEVWQVRASYDQKPYRRLMMQTWGVKVHPSPSDLTDAGRKL 240
           GAGQWGSALAFACSIFGLGCEVWQVRASYDQKPYRRLMMQTWGVKVHPSPSDLTDAGRKL
Sbjct: 181 GAGQWGSALAFACSIFGLGCEVWQVRASYDQKPYRRLMMQTWGVKVHPSPSDLTDAGRKL 240

Query: 241 LQVDPSSPGSLGIAISEAVEVAALNADTKYCLGSVLNHVLLHQTVIGEECLKQMEALGES 300
           LQVDPSSPGSLGIAISEAVEVAALNADTKYCLGSVLNHVLLHQTVIGEECLKQMEALGES
Sbjct: 241 LQVDPSSPGSLGIAISEAVEVAALNADTKYCLGSVLNHVLLHQTVIGEECLKQMEALGES 300

Query: 301 PDVIIGCTGGGSNFAGLSFPFLREKLAGKINPILRAVEPAACPSLTKGVYAYDFGDTAGM 360
           PDVIIGCTGGGSNFAGLSFPFLREKLAGKINPILRAVEPAACPSLTKGVYAYDFGDTAGM
Sbjct: 301 PDVIIGCTGGGSNFAGLSFPFLREKLAGKINPILRAVEPAACPSLTKGVYAYDFGDTAGM 360

Query: 361 TPLMKMHTLGHNFIPDPIHAGGLRYHGMAPLISHVYNLGLLEAISLPQTECFQGAIQFAR 420
           TPLMKMHTLGHNFIPDPIHAGGLRYHGMAPLISHVYNLGLLEAISLPQTECFQGAIQFAR
Sbjct: 361 TPLMKMHTLGHNFIPDPIHAGGLRYHGMAPLISHVYNLGLLEAISLPQTECFQGAIQFAR 420

Query: 421 SEGLIAAPEPTHAIAATIREALRCRETGESKVILTAMCGHGHFDLPAYEKYLQGGMTDLS 480
           SEGLIAAPEPTHAIAATIREALRCRETGESKVILTAMCGHGHFDLPAYEKYLQGGMTDLS
Sbjct: 421 SEGLIAAPEPTHAIAATIREALRCRETGESKVILTAMCGHGHFDLPAYEKYLQGGMTDLS 480

Query: 481 FAEEKIQESLTTIPQVA 498
           FAEEKIQESLTTIPQVA
Sbjct: 481 FAEEKIQESLTTIPQVA 497

BLAST of Moc04g37340 vs. NCBI nr
Match: XP_038890851.1 (tryptophan synthase beta chain 2 [Benincasa hispida])

HSP 1 Score: 929.9 bits (2402), Expect = 9.4e-267
Identity = 454/497 (91.35%), Postives = 475/497 (95.57%), Query Frame = 0

Query: 1   MASHFLVAASSTSPAI-NGRVGEELPGRLSLKRNPRNLRFSNGYRTRAASNRNLKPVEVP 60
           MA+ FL   S TS  I NGRVGE+L G LSLKR PR+LRFSNGYRT+AASN NLK VEVP
Sbjct: 1   MAATFLAVGSLTSSGINNGRVGEQLSGYLSLKRVPRHLRFSNGYRTKAASNPNLKAVEVP 60

Query: 61  RRWYNLVADLPVKPPPPLHPKTFQPIKPQDLSPLFPDALIEQEASTDRYINIPDEVVDIY 120
           R+WYNLVADLPVKPPPPLHPKT +PIKP+DLS LFPD LI+QEASTD+YINIPDEVVDIY
Sbjct: 61  RQWYNLVADLPVKPPPPLHPKTLEPIKPEDLSHLFPDELIKQEASTDQYINIPDEVVDIY 120

Query: 121 GLWRPTPLIRAKRLEKLLDTPARIYYKYEGVSPAGSHKANSAVPQVWYNVQEGVKNVVTE 180
           GLWRPTPLIRAKRLEKLLDTPARIYYKYEGVSPAGSHK NSAVPQV+YNVQEGVKNVVTE
Sbjct: 121 GLWRPTPLIRAKRLEKLLDTPARIYYKYEGVSPAGSHKPNSAVPQVYYNVQEGVKNVVTE 180

Query: 181 TGAGQWGSALAFACSIFGLGCEVWQVRASYDQKPYRRLMMQTWGVKVHPSPSDLTDAGRK 240
           TGAGQWG +LAFACSIFG+GCEVWQVRASYDQKPYRR+MM+TWG KVHPSPSD+TDAGRK
Sbjct: 181 TGAGQWGCSLAFACSIFGIGCEVWQVRASYDQKPYRRMMMETWGAKVHPSPSDMTDAGRK 240

Query: 241 LLQVDPSSPGSLGIAISEAVEVAALNADTKYCLGSVLNHVLLHQTVIGEECLKQMEALGE 300
            LQVDPSSPGSLGIAISEAVEVAALNADTKYCLGSVLNHVLLHQTVIGEECLKQMEALGE
Sbjct: 241 FLQVDPSSPGSLGIAISEAVEVAALNADTKYCLGSVLNHVLLHQTVIGEECLKQMEALGE 300

Query: 301 SPDVIIGCTGGGSNFAGLSFPFLREKLAGKINPILRAVEPAACPSLTKGVYAYDFGDTAG 360
           SPDVIIGCTGGGSNFAGLSFPFLREKLAGKINP++RAVEPAACPSLTKGVYAYD+GDTAG
Sbjct: 301 SPDVIIGCTGGGSNFAGLSFPFLREKLAGKINPVIRAVEPAACPSLTKGVYAYDYGDTAG 360

Query: 361 MTPLMKMHTLGHNFIPDPIHAGGLRYHGMAPLISHVYNLGLLEAISLPQTECFQGAIQFA 420
           MTPLMKMHTLGHNFIPDPIHAGGLRYHGMAPLISHVYNLGLLEAISLPQTECFQGAIQFA
Sbjct: 361 MTPLMKMHTLGHNFIPDPIHAGGLRYHGMAPLISHVYNLGLLEAISLPQTECFQGAIQFA 420

Query: 421 RSEGLIAAPEPTHAIAATIREALRCRETGESKVILTAMCGHGHFDLPAYEKYLQGGMTDL 480
           RSEGLI APEPTHAIAATI+EALRCRETGESKVILTAMCGHGHFDLPAYEKYLQGG+TDL
Sbjct: 421 RSEGLIPAPEPTHAIAATIQEALRCRETGESKVILTAMCGHGHFDLPAYEKYLQGGITDL 480

Query: 481 SFAEEKIQESLTTIPQV 497
           SFA+EKIQESL TIPQV
Sbjct: 481 SFAKEKIQESLITIPQV 497

BLAST of Moc04g37340 vs. NCBI nr
Match: XP_031741291.1 (uncharacterized protein LOC101219640 [Cucumis sativus])

HSP 1 Score: 927.9 bits (2397), Expect = 3.6e-266
Identity = 450/497 (90.54%), Postives = 474/497 (95.37%), Query Frame = 0

Query: 1   MASHFLVAASSTSPAINGRVGEELPGRLSLKRNPRNLRFSNGYRTRAASNRNLKPVEVPR 60
           MA+ FL   S TS +IN  VGE+LPG LSLKR P +LRFSNGYRT+AASN NLK VEVPR
Sbjct: 1   MAATFLTVGSLTSSSIN-NVGEQLPGCLSLKRVPAHLRFSNGYRTKAASNPNLKAVEVPR 60

Query: 61  RWYNLVADLPVKPPPPLHPKTFQPIKPQDLSPLFPDALIEQEASTDRYINIPDEVVDIYG 120
           +WYNLVADLPVKPPPPLHPKT++PIKP+DLS LFPD LI+QEAST+RYINIPDEVVDIYG
Sbjct: 61  QWYNLVADLPVKPPPPLHPKTYEPIKPEDLSHLFPDELIKQEASTERYINIPDEVVDIYG 120

Query: 121 LWRPTPLIRAKRLEKLLDTPARIYYKYEGVSPAGSHKANSAVPQVWYNVQEGVKNVVTET 180
           LWRPTPLIRAKRLEKLLDTPARIYYKYEGVSPAGSHK NSAVPQVWYN QEGVKNVVTET
Sbjct: 121 LWRPTPLIRAKRLEKLLDTPARIYYKYEGVSPAGSHKPNSAVPQVWYNAQEGVKNVVTET 180

Query: 181 GAGQWGSALAFACSIFGLGCEVWQVRASYDQKPYRRLMMQTWGVKVHPSPSDLTDAGRKL 240
           GAGQWG +LAFACSIFG+GCEVWQVRASYDQKPYRR+MM+TWG KVHPSPSD+TDAGRK 
Sbjct: 181 GAGQWGCSLAFACSIFGIGCEVWQVRASYDQKPYRRMMMETWGAKVHPSPSDITDAGRKF 240

Query: 241 LQVDPSSPGSLGIAISEAVEVAALNADTKYCLGSVLNHVLLHQTVIGEECLKQMEALGES 300
           LQVDPSSPGSLGIAISEAVEVAALNADTKYCLGSVLNHVLLHQTVIGEECLKQ+EALGE+
Sbjct: 241 LQVDPSSPGSLGIAISEAVEVAALNADTKYCLGSVLNHVLLHQTVIGEECLKQLEALGET 300

Query: 301 PDVIIGCTGGGSNFAGLSFPFLREKLAGKINPILRAVEPAACPSLTKGVYAYDFGDTAGM 360
           PD+IIGCTGGGSNFAGLSFPFLREKLAG INP++RAVEPAACPSLTKGVYAYD+GDTAGM
Sbjct: 301 PDLIIGCTGGGSNFAGLSFPFLREKLAGNINPVIRAVEPAACPSLTKGVYAYDYGDTAGM 360

Query: 361 TPLMKMHTLGHNFIPDPIHAGGLRYHGMAPLISHVYNLGLLEAISLPQTECFQGAIQFAR 420
           TPLMKMHTLGHNFIPDPIHAGGLRYHGMAPLISHVYNLGLLEAISLPQTECFQGAIQFAR
Sbjct: 361 TPLMKMHTLGHNFIPDPIHAGGLRYHGMAPLISHVYNLGLLEAISLPQTECFQGAIQFAR 420

Query: 421 SEGLIAAPEPTHAIAATIREALRCRETGESKVILTAMCGHGHFDLPAYEKYLQGGMTDLS 480
           SEGLI APEPTHAIAATIREALRCRETGESKVILTAMCGHGHFDLPAYEKYLQGG++DLS
Sbjct: 421 SEGLIPAPEPTHAIAATIREALRCRETGESKVILTAMCGHGHFDLPAYEKYLQGGISDLS 480

Query: 481 FAEEKIQESLTTIPQVA 498
           FA EKIQESLTTIPQVA
Sbjct: 481 FANEKIQESLTTIPQVA 496

BLAST of Moc04g37340 vs. NCBI nr
Match: XP_008459440.1 (PREDICTED: tryptophan synthase beta chain 2-like [Cucumis melo])

HSP 1 Score: 924.1 bits (2387), Expect = 5.2e-265
Identity = 446/497 (89.74%), Postives = 474/497 (95.37%), Query Frame = 0

Query: 1   MASHFLVAASSTSPAINGRVGEELPGRLSLKRNPRNLRFSNGYRTRAASNRNLKPVEVPR 60
           MA+ FL   S TS +IN  VGE+LPG LSLKR P +LRFSNGYRT++ASN NLK VE+PR
Sbjct: 1   MAATFLTVGSLTSSSIN-NVGEQLPGCLSLKRVPTHLRFSNGYRTKSASNPNLKAVEIPR 60

Query: 61  RWYNLVADLPVKPPPPLHPKTFQPIKPQDLSPLFPDALIEQEASTDRYINIPDEVVDIYG 120
           +WYNLVADLPVKPPPPLHPKT++PIKP+DLS LFPD LI+QEAST+RYINIPDEVVDIYG
Sbjct: 61  QWYNLVADLPVKPPPPLHPKTYEPIKPEDLSHLFPDELIKQEASTERYINIPDEVVDIYG 120

Query: 121 LWRPTPLIRAKRLEKLLDTPARIYYKYEGVSPAGSHKANSAVPQVWYNVQEGVKNVVTET 180
           LWRPTPLIRAKRLEKLLDTPARIYYKYEGVSPAGSHK NSAVPQVWYN QEGVKNVVTET
Sbjct: 121 LWRPTPLIRAKRLEKLLDTPARIYYKYEGVSPAGSHKPNSAVPQVWYNAQEGVKNVVTET 180

Query: 181 GAGQWGSALAFACSIFGLGCEVWQVRASYDQKPYRRLMMQTWGVKVHPSPSDLTDAGRKL 240
           GAGQWG +LAFACSIFG+GCEVWQVRASYDQKPYRR+MM+TWG KVHPSPSD+TDAGRK 
Sbjct: 181 GAGQWGCSLAFACSIFGIGCEVWQVRASYDQKPYRRMMMETWGAKVHPSPSDVTDAGRKF 240

Query: 241 LQVDPSSPGSLGIAISEAVEVAALNADTKYCLGSVLNHVLLHQTVIGEECLKQMEALGES 300
           LQVDPSSPGSLGIAISEAVEVAALNADTKYCLGSVLNHVLLHQTVIGEECLKQ+EALGES
Sbjct: 241 LQVDPSSPGSLGIAISEAVEVAALNADTKYCLGSVLNHVLLHQTVIGEECLKQLEALGES 300

Query: 301 PDVIIGCTGGGSNFAGLSFPFLREKLAGKINPILRAVEPAACPSLTKGVYAYDFGDTAGM 360
           PD+IIGCTGGGSNFAGLSFPFLREKLAG INP++RAVEPAACPSLTKGVYAYD+GDTAGM
Sbjct: 301 PDLIIGCTGGGSNFAGLSFPFLREKLAGNINPVIRAVEPAACPSLTKGVYAYDYGDTAGM 360

Query: 361 TPLMKMHTLGHNFIPDPIHAGGLRYHGMAPLISHVYNLGLLEAISLPQTECFQGAIQFAR 420
           TPLMKMHTLGHNFIPDPIHAGGLRYHGMAPLISHVYNLGLLEA+SLPQTECFQGAI+FAR
Sbjct: 361 TPLMKMHTLGHNFIPDPIHAGGLRYHGMAPLISHVYNLGLLEAVSLPQTECFQGAIKFAR 420

Query: 421 SEGLIAAPEPTHAIAATIREALRCRETGESKVILTAMCGHGHFDLPAYEKYLQGGMTDLS 480
           SEGLI APEPTHAIAATIREALRCRETGESKVILTAMCGHGHFDLPAYEKYLQGG+TDLS
Sbjct: 421 SEGLIPAPEPTHAIAATIREALRCRETGESKVILTAMCGHGHFDLPAYEKYLQGGITDLS 480

Query: 481 FAEEKIQESLTTIPQVA 498
           FA EK++ESLTTIPQVA
Sbjct: 481 FANEKMKESLTTIPQVA 496

BLAST of Moc04g37340 vs. NCBI nr
Match: XP_022993275.1 (uncharacterized protein LOC111489338 [Cucurbita maxima])

HSP 1 Score: 904.4 bits (2336), Expect = 4.2e-259
Identity = 438/490 (89.39%), Postives = 466/490 (95.10%), Query Frame = 0

Query: 7   VAASSTSPAINGRVGEELPGRLSLKRNPRNLRFSNGYRTRAASNRNLKPVEVPRRWYNLV 66
           + ASS S  ING       G LS++R PR+LRFSNGYRT+AASN NLK VEVPR+WYNLV
Sbjct: 1   MVASSISSGING------GGCLSIERIPRHLRFSNGYRTKAASNPNLKAVEVPRQWYNLV 60

Query: 67  ADLPVKPPPPLHPKTFQPIKPQDLSPLFPDALIEQEASTDRYINIPDEVVDIYGLWRPTP 126
           ADLPVKPPPPLHPKTF+PIKP+DLSPLFPD LI+QEAS +RYI+IPDEVVDIYGLWRPTP
Sbjct: 61  ADLPVKPPPPLHPKTFEPIKPEDLSPLFPDELIKQEASAERYIDIPDEVVDIYGLWRPTP 120

Query: 127 LIRAKRLEKLLDTPARIYYKYEGVSPAGSHKANSAVPQVWYNVQEGVKNVVTETGAGQWG 186
           LIRAKRLEKLLDTPARIYYKYEGVSPAGSHK NSAVPQVWYNVQEGVKNVVTETGAGQWG
Sbjct: 121 LIRAKRLEKLLDTPARIYYKYEGVSPAGSHKPNSAVPQVWYNVQEGVKNVVTETGAGQWG 180

Query: 187 SALAFACSIFGLGCEVWQVRASYDQKPYRRLMMQTWGVKVHPSPSDLTDAGRKLLQVDPS 246
            +LAFACS+FG+GCEVWQVRASYDQKPYRR+MM+TWG KVHPSPS++TD+GRK LQVDPS
Sbjct: 181 CSLAFACSLFGIGCEVWQVRASYDQKPYRRMMMETWGAKVHPSPSNVTDSGRKFLQVDPS 240

Query: 247 SPGSLGIAISEAVEVAALNADTKYCLGSVLNHVLLHQTVIGEECLKQMEALGESPDVIIG 306
           SPGSLGIAISEAVEVAALNADTKYCLGSVLNHVLLHQTVIGEECLKQMEALGE+PDVIIG
Sbjct: 241 SPGSLGIAISEAVEVAALNADTKYCLGSVLNHVLLHQTVIGEECLKQMEALGETPDVIIG 300

Query: 307 CTGGGSNFAGLSFPFLREKLAGKINPILRAVEPAACPSLTKGVYAYDFGDTAGMTPLMKM 366
           CTGGGSNFAGLSFPFLREKLAGKI+P++RAVEPAACPSLTKGVYAYD+GDTAGMTPLMKM
Sbjct: 301 CTGGGSNFAGLSFPFLREKLAGKISPVIRAVEPAACPSLTKGVYAYDYGDTAGMTPLMKM 360

Query: 367 HTLGHNFIPDPIHAGGLRYHGMAPLISHVYNLGLLEAISLPQTECFQGAIQFARSEGLIA 426
           HTLGHNFIPDPIHAGGLRYHGMAPLISHVY+LGLLEA+SLPQTECFQGAIQFARSEGLI 
Sbjct: 361 HTLGHNFIPDPIHAGGLRYHGMAPLISHVYDLGLLEAVSLPQTECFQGAIQFARSEGLIP 420

Query: 427 APEPTHAIAATIREALRCRETGESKVILTAMCGHGHFDLPAYEKYLQGGMTDLSFAEEKI 486
           APEPTHAIA TIREALRCRETGESKVILTAMCGHGHFDL AYEKYLQGGMTDLSFAEEKI
Sbjct: 421 APEPTHAIAETIREALRCRETGESKVILTAMCGHGHFDLAAYEKYLQGGMTDLSFAEEKI 480

Query: 487 QESLTTIPQV 497
           +ESLTTIPQ+
Sbjct: 481 KESLTTIPQL 484

BLAST of Moc04g37340 vs. ExPASy Swiss-Prot
Match: O67409 (Tryptophan synthase beta chain 2 OS=Aquifex aeolicus (strain VF5) OX=224324 GN=trpB2 PE=3 SV=1)

HSP 1 Score: 547.0 bits (1408), Expect = 2.2e-154
Identity = 263/425 (61.88%), Postives = 323/425 (76.00%), Query Frame = 0

Query: 57  EVPRRWYNLVADLPVKPPPPLHPKTFQPIKPQDLSPLFPDALIEQEASTDRYINIPDEVV 116
           E+P++W N++  LP    PPL P+T +P+KP+ L  +FP+ L+EQE S   +I+IP+EV+
Sbjct: 10  EIPKKWLNILPLLPEPLEPPLDPETMEPVKPEKLLAIFPEPLVEQEVSDKEWIDIPEEVL 69

Query: 117 DIYGLWRPTPLIRAKRLEKLLDTPARIYYKYEGVSPAGSHKANSAVPQVWYNVQEGVKNV 176
           DIY LWRPTPL RAK LE+ L TPA+I+YK E VSP GSHK N+AV Q +YN   GVK +
Sbjct: 70  DIYSLWRPTPLHRAKNLEEFLGTPAKIFYKNESVSPPGSHKPNTAVAQAYYNKISGVKRL 129

Query: 177 VTETGAGQWGSALAFACSIFGLGCEVWQVRASYDQKPYRRLMMQTWGVKVHPSPSDLTDA 236
            TETGAGQWGSAL+FA   F L C V+ VR SY+QKPYRR++M+TW  +V PSPS  T+A
Sbjct: 130 TTETGAGQWGSALSFATQFFDLQCRVYMVRVSYNQKPYRRILMETWKGEVIPSPSPYTNA 189

Query: 237 GRKLLQVDPSSPGSLGIAISEAVEVAALNADTKYCLGSVLNHVLLHQTVIGEECLKQMEA 296
           GRK  + +P  PGSLGIAISEA+E AA   DTKY LGSVLNHVLLHQTVIG E  KQME 
Sbjct: 190 GRKYYEENPEHPGSLGIAISEAIEEAASREDTKYSLGSVLNHVLLHQTVIGLEAKKQMEE 249

Query: 297 LGESPDVIIGCTGGGSNFAGLSFPFLREKLAG---KINPILRAVEPAACPSLTKGVYAYD 356
            G  PDVIIG  GGGSNFAGLSFPFL + L G   K +  + AVEP ACP+LTKG Y YD
Sbjct: 250 AGYYPDVIIGAVGGGSNFAGLSFPFLADVLRGDKRKEDLKVLAVEPEACPTLTKGEYKYD 309

Query: 357 FGDTAGMTPLMKMHTLGHNFIPDPIHAGGLRYHGMAPLISHVYNLGLLEAISLPQTECFQ 416
           FGD+ G+TPL+KM+TLGH+F+P PIHAGGLRYHG APL+  +YNLG ++A++  QTE F+
Sbjct: 310 FGDSVGLTPLIKMYTLGHDFVPSPIHAGGLRYHGDAPLVCKLYNLGYIDAVAYKQTEVFE 369

Query: 417 GAIQFARSEGLIAAPEPTHAIAATIREALRCRETGESKVILTAMCGHGHFDLPAYEKYLQ 476
            A+ FAR+EG++ APE  HAI A I EAL+C+ETGE KVIL  + GHG+FDL AY+KYL 
Sbjct: 370 AAVTFARTEGIVPAPESAHAIKAAIDEALKCKETGEEKVILFNLSGHGYFDLSAYDKYLH 429

Query: 477 GGMTD 479
           G +TD
Sbjct: 430 GELTD 434

BLAST of Moc04g37340 vs. ExPASy Swiss-Prot
Match: Q8TL44 (Tryptophan synthase beta chain 2 OS=Methanosarcina acetivorans (strain ATCC 35395 / DSM 2834 / JCM 12185 / C2A) OX=188937 GN=trpB2 PE=3 SV=1)

HSP 1 Score: 538.1 bits (1385), Expect = 1.0e-151
Identity = 250/418 (59.81%), Postives = 323/418 (77.27%), Query Frame = 0

Query: 57  EVPRRWYNLVADLPVKPPPPLHPKTFQPIKPQDLSPLFPDALIEQEASTDRYINIPDEVV 116
           E+P++WYN++ADLP    PPL P+T+QPI P  L P+FP ALI QE S+DRYI+IP+EV+
Sbjct: 22  EMPKKWYNVLADLPSPIDPPLDPRTWQPISPDALEPIFPKALIMQEMSSDRYIDIPEEVL 81

Query: 117 DIYGLWRPTPLIRAKRLEKLLDTPARIYYKYEGVSPAGSHKANSAVPQVWYNVQEGVKNV 176
           D+Y LWRP+PL RA +LEK+L +PA+IYYKYEGVSPAGSHK N+++ Q +YN++EG + +
Sbjct: 82  DVYRLWRPSPLFRAHQLEKVLKSPAKIYYKYEGVSPAGSHKTNTSIAQAYYNMKEGTERL 141

Query: 177 VTETGAGQWGSALAFACSIFGLGCEVWQVRASYDQKPYRRLMMQTWGVKVHPSPSDLTDA 236
            TETGAGQWGSAL+ AC+ F L C+V+ VR+S+ QKPYR+ ++  WG  V PSPS  T+ 
Sbjct: 142 TTETGAGQWGSALSLACNYFDLECKVYMVRSSFYQKPYRKSLITLWGGNVVPSPSPDTEF 201

Query: 237 GRKLLQVDPSSPGSLGIAISEAVEVAALNADTKYCLGSVLNHVLLHQTVIGEECLKQMEA 296
           GRK+LQ  P +PGSLGIAISEAVE A  + +TKY LGSVLNHV+LHQTVIG EC +Q+  
Sbjct: 202 GRKILQEQPDTPGSLGIAISEAVEDAIAHENTKYSLGSVLNHVVLHQTVIGAECKQQLAQ 261

Query: 297 LGESPDVIIGCTGGGSNFAGLSFPFLREKLAGKINPILRAVEPAACPSLTKGVYAYDFGD 356
           + E PDV+IGC GGGSN  G+   F++++L GK +  + AVEP+ACPSLTKG Y YDFGD
Sbjct: 262 VEEYPDVVIGCCGGGSNLGGIGLEFIKDRLEGKHSARVVAVEPSACPSLTKGEYRYDFGD 321

Query: 357 TAGMTPLMKMHTLGHNFIPDPIHAGGLRYHGMAPLISHVYNLGLLEAISLPQTECFQGAI 416
           TA MTPL+KM+TLGH  +P  IHAGGLRYHG +P+IS + + GL+EA+S  Q E F  A+
Sbjct: 322 TAEMTPLLKMYTLGHKHVPPAIHAGGLRYHGDSPIISKLCSEGLMEAVSYDQQEVFDAAV 381

Query: 417 QFARSEGLIAAPEPTHAIAATIREALRCRETGESKVILTAMCGHGHFDLPAYEKYLQG 475
           QFAR+EG++ APE +HAI   I EAL  ++TGE K IL  + GHGHFD+ +Y+KY  G
Sbjct: 382 QFARTEGIVPAPESSHAIRCAIDEALAAKQTGEEKTILFNLSGHGHFDMSSYDKYFSG 439

BLAST of Moc04g37340 vs. ExPASy Swiss-Prot
Match: Q8Q001 (Tryptophan synthase beta chain 2 OS=Methanosarcina mazei (strain ATCC BAA-159 / DSM 3647 / Goe1 / Go1 / JCM 11833 / OCM 88) OX=192952 GN=trpB2 PE=3 SV=1)

HSP 1 Score: 537.7 bits (1384), Expect = 1.4e-151
Identity = 253/418 (60.53%), Postives = 320/418 (76.56%), Query Frame = 0

Query: 57  EVPRRWYNLVADLPVKPPPPLHPKTFQPIKPQDLSPLFPDALIEQEASTDRYINIPDEVV 116
           E+P+RWYN+++DLP    PPL P+T+QPI P+ L P+F   LI QE S+DRYI+IP E++
Sbjct: 22  EMPKRWYNVLSDLPSPIDPPLDPRTWQPINPEALEPIFAKELIRQEMSSDRYIDIPAEIL 81

Query: 117 DIYGLWRPTPLIRAKRLEKLLDTPARIYYKYEGVSPAGSHKANSAVPQVWYNVQEGVKNV 176
           D+Y LWRP+PL RA +LEK L TPA+IYYKYEGVSPAGSHK N+++ Q +YN++EG + +
Sbjct: 82  DVYRLWRPSPLFRAHQLEKDLKTPAKIYYKYEGVSPAGSHKTNTSIAQAYYNMKEGTERL 141

Query: 177 VTETGAGQWGSALAFACSIFGLGCEVWQVRASYDQKPYRRLMMQTWGVKVHPSPSDLTDA 236
            TETGAGQWGSAL+ AC+ F L C+V+ VR+SY QKPYR+ +M  WG  V PSPS  T+ 
Sbjct: 142 TTETGAGQWGSALSLACNYFDLECKVYMVRSSYYQKPYRKSLMTLWGGNVVPSPSPDTEF 201

Query: 237 GRKLLQVDPSSPGSLGIAISEAVEVAALNADTKYCLGSVLNHVLLHQTVIGEECLKQMEA 296
           GRK+L+  P +PGSLGIAISEAVE A  + +TKY LGSVLNHV+LHQTVIG EC KQ+E 
Sbjct: 202 GRKILKEQPDTPGSLGIAISEAVEDAIAHDNTKYTLGSVLNHVVLHQTVIGAECKKQLEQ 261

Query: 297 LGESPDVIIGCTGGGSNFAGLSFPFLREKLAGKINPILRAVEPAACPSLTKGVYAYDFGD 356
           + E PDV+IGC GGGSN  G+   F++++L GK N  + AVEP+ACPSLTKG Y YDFGD
Sbjct: 262 VEEYPDVVIGCCGGGSNLGGIGLEFIKDRLEGKHNARVVAVEPSACPSLTKGEYRYDFGD 321

Query: 357 TAGMTPLMKMHTLGHNFIPDPIHAGGLRYHGMAPLISHVYNLGLLEAISLPQTECFQGAI 416
           TA MTPL+KM+TLGH  IP  IHAGGLRYHG +P+IS +   GL+EA+S  Q E F  A+
Sbjct: 322 TAEMTPLLKMYTLGHKHIPPAIHAGGLRYHGDSPIISKLCAEGLMEAVSYGQKEVFDAAV 381

Query: 417 QFARSEGLIAAPEPTHAIAATIREALRCRETGESKVILTAMCGHGHFDLPAYEKYLQG 475
           QFAR+EG++ APE +HAI   I EAL  ++ GE KVIL  + GHGHFD+ +Y+KY  G
Sbjct: 382 QFARTEGIVPAPESSHAIRCAIDEALEAKQKGEEKVILFNLSGHGHFDMASYDKYFSG 439

BLAST of Moc04g37340 vs. ExPASy Swiss-Prot
Match: O29028 (Tryptophan synthase beta chain 2 OS=Archaeoglobus fulgidus (strain ATCC 49558 / VC-16 / DSM 4304 / JCM 9628 / NBRC 100126) OX=224325 GN=trpB2 PE=3 SV=1)

HSP 1 Score: 535.0 bits (1377), Expect = 8.8e-151
Identity = 252/422 (59.72%), Postives = 319/422 (75.59%), Query Frame = 0

Query: 57  EVPRRWYNLVADLPVKPPPPLHPKTFQPIKPQDLSPLFPDALIEQEASTDRYINIPDEVV 116
           E+P+ WYN++ DLP   PPPLHP T +P+KP+DL P+FP  LI+QE S +R+I IP++V 
Sbjct: 15  EMPKEWYNVLPDLPEPLPPPLHPATQEPVKPEDLEPIFPKGLIQQEMSGERWIRIPEDVR 74

Query: 117 DIYGLWRPTPLIRAKRLEKLLDTPARIYYKYEGVSPAGSHKANSAVPQVWYNVQEGVKNV 176
           +IY +WRPTPL+RA+RLEK L TPARIY+KYEG SP GSHK N+AV Q +YN +EGV+ +
Sbjct: 75  EIYRIWRPTPLVRAERLEKALKTPARIYFKYEGASPPGSHKPNTAVAQAYYNAKEGVERL 134

Query: 177 VTETGAGQWGSALAFACSIFGLGCEVWQVRASYDQKPYRRLMMQTWGVKVHPSPSDLTDA 236
            TETGAGQWGSAL FA  +F + C V+ V+ S+ QKPYRR+MM+TWG +V PSPSD T+ 
Sbjct: 135 TTETGAGQWGSALCFATKLFEMACTVYMVKVSFMQKPYRRVMMETWGGEVIPSPSDRTEV 194

Query: 237 GRKLLQVDPSSPGSLGIAISEAVEVAALNADTKYCLGSVLNHVLLHQTVIGEECLKQMEA 296
           GRK+L  +P +PGSLGIAISEA+E AA N +TKY LGSVLNHVLLHQTVIG E   Q+E 
Sbjct: 195 GRKILAENPDTPGSLGIAISEAIEDAAKNENTKYSLGSVLNHVLLHQTVIGLETKAQLEK 254

Query: 297 LGESPDVIIGCTGGGSNFAGLSFPFLREKLAGKINPILRAVEPAACPSLTKGVYAYDFGD 356
           + E PDV+IGC GGGSNFAGL++PF+ +   G +  I  AVEPAACP+LT G Y YDFGD
Sbjct: 255 VDEKPDVLIGCVGGGSNFAGLTYPFVNDAKNGDLEII--AVEPAACPTLTAGEYKYDFGD 314

Query: 357 TAGMTPLMKMHTLGHNFIPDPIHAGGLRYHGMAPLISHVYNLGLLEAISLPQTECFQGAI 416
            AG+TPL+KM+TLGH+FIP PIHAGGLRYHG AP +  +   G+++A ++ Q   F+  +
Sbjct: 315 VAGLTPLLKMYTLGHDFIPPPIHAGGLRYHGDAPTLCMLVKHGVIKARAVKQLPTFEAGL 374

Query: 417 QFARSEGLIAAPEPTHAIAATIREALRCRETGESKVILTAMCGHGHFDLPAYEKYLQGGM 476
            FAR+EG+I APE  HA+ A I EA++ RE  E KVI+    GHG  DL AY+ YL G +
Sbjct: 375 LFARTEGIIPAPETNHAVRAAIDEAIKARENNEEKVIVFGFSGHGLLDLQAYDDYLAGRL 434

Query: 477 TD 479
            D
Sbjct: 435 AD 434

BLAST of Moc04g37340 vs. ExPASy Swiss-Prot
Match: Q9WZ09 (Tryptophan synthase beta chain 2 OS=Thermotoga maritima (strain ATCC 43589 / DSM 3109 / JCM 10099 / NBRC 100826 / MSB8) OX=243274 GN=trpB2 PE=3 SV=1)

HSP 1 Score: 512.3 bits (1318), Expect = 6.1e-144
Identity = 253/417 (60.67%), Postives = 315/417 (75.54%), Query Frame = 0

Query: 52  NLKPVEVPRRWYNLVADLPVKPPPPLHPKTFQPIKPQDLSPLFPDALIEQEASTDRYINI 111
           NLKP E+P+ WYN++ADLP K  PPL P+T QPI P+ LS +FP +LIEQE S +R+I I
Sbjct: 6   NLKPEEIPKHWYNVLADLPFKLDPPLDPETKQPISPEKLSVIFPMSLIEQEVSEERFIEI 65

Query: 112 PDEVVDIYGLWRPTPLIRAKRLEKLLDTPARIYYKYEGVSPAGSHKANSAVPQVWYNVQE 171
           P+ V+  Y ++RPTPLIRA  LE+ L TPARIYYKYEGVSP GSHK N+A+ Q +YN  E
Sbjct: 66  PEPVLKEYAVYRPTPLIRATFLEEYLQTPARIYYKYEGVSPTGSHKPNTAIAQAYYNKIE 125

Query: 172 GVKNVVTETGAGQWGSALAFACSIFGLGCEVWQVRASYDQKPYRRLMMQTWGVKVHPSPS 231
           GVK +VTETGAGQWGSAL++A + FGL  +V+ V+ SY QKP R+ MM  +G KV PSPS
Sbjct: 126 GVKRLVTETGAGQWGSALSYAGAKFGLEVKVFMVKVSYQQKPMRKYMMNLFGGKVTPSPS 185

Query: 232 DLTDAGRKLLQVDPSSPGSLGIAISEAVEVAALNADTKYCLGSVLNHVLLHQTVIGEECL 291
           + T+ GRK+L  DP +PGSLGIAISEA+EVA  + +TKY LGSVLNHVLLHQTVIG E  
Sbjct: 186 EETNFGRKILSEDPDNPGSLGIAISEALEVAVSDPNTKYSLGSVLNHVLLHQTVIGLEIK 245

Query: 292 KQMEALGESPDVIIGCTGGGSNFAGLSFPFLREKLAGKINPILRAVEPAACPSLTKGVYA 351
           KQ+E +GE PD+++GC GGGSNF G   PF+ +KL+G+    + A EPAACPSLTKG Y 
Sbjct: 246 KQLELIGEKPDILLGCHGGGSNFGGTILPFVPDKLSGRDIRFV-ACEPAACPSLTKGNYD 305

Query: 352 YDFGDTAGMTPLMKMHTLGHNFIPDPIHAGGLRYHGMAPLISHVYNLGLLEAISLPQTEC 411
           YDFGDTAG+TPL+KM+TLG +FIP  IHAGGLRYHG AP+I+ +   GL+EA +  Q E 
Sbjct: 306 YDFGDTAGLTPLLKMYTLGKDFIPPKIHAGGLRYHGSAPIIARLVKEGLVEAQAFDQDET 365

Query: 412 FQGAIQFARSEGLIAAPEPTHAIAATIREALRCRETGESKVILTAMCGHGHFDLPAY 469
           F+ A  FA+ EG+I APE  HAIA  IREA + +E G+ +VI+  + GHG  DL AY
Sbjct: 366 FEAAKIFAKLEGIIPAPESAHAIAGAIREAKKAKEEGKERVIVFTLSGHGLLDLTAY 421

BLAST of Moc04g37340 vs. ExPASy TrEMBL
Match: A0A6J1BXI7 (Tryptophan synthase OS=Momordica charantia OX=3673 GN=LOC111006607 PE=3 SV=1)

HSP 1 Score: 1012.7 bits (2617), Expect = 5.3e-292
Identity = 497/497 (100.00%), Postives = 497/497 (100.00%), Query Frame = 0

Query: 1   MASHFLVAASSTSPAINGRVGEELPGRLSLKRNPRNLRFSNGYRTRAASNRNLKPVEVPR 60
           MASHFLVAASSTSPAINGRVGEELPGRLSLKRNPRNLRFSNGYRTRAASNRNLKPVEVPR
Sbjct: 1   MASHFLVAASSTSPAINGRVGEELPGRLSLKRNPRNLRFSNGYRTRAASNRNLKPVEVPR 60

Query: 61  RWYNLVADLPVKPPPPLHPKTFQPIKPQDLSPLFPDALIEQEASTDRYINIPDEVVDIYG 120
           RWYNLVADLPVKPPPPLHPKTFQPIKPQDLSPLFPDALIEQEASTDRYINIPDEVVDIYG
Sbjct: 61  RWYNLVADLPVKPPPPLHPKTFQPIKPQDLSPLFPDALIEQEASTDRYINIPDEVVDIYG 120

Query: 121 LWRPTPLIRAKRLEKLLDTPARIYYKYEGVSPAGSHKANSAVPQVWYNVQEGVKNVVTET 180
           LWRPTPLIRAKRLEKLLDTPARIYYKYEGVSPAGSHKANSAVPQVWYNVQEGVKNVVTET
Sbjct: 121 LWRPTPLIRAKRLEKLLDTPARIYYKYEGVSPAGSHKANSAVPQVWYNVQEGVKNVVTET 180

Query: 181 GAGQWGSALAFACSIFGLGCEVWQVRASYDQKPYRRLMMQTWGVKVHPSPSDLTDAGRKL 240
           GAGQWGSALAFACSIFGLGCEVWQVRASYDQKPYRRLMMQTWGVKVHPSPSDLTDAGRKL
Sbjct: 181 GAGQWGSALAFACSIFGLGCEVWQVRASYDQKPYRRLMMQTWGVKVHPSPSDLTDAGRKL 240

Query: 241 LQVDPSSPGSLGIAISEAVEVAALNADTKYCLGSVLNHVLLHQTVIGEECLKQMEALGES 300
           LQVDPSSPGSLGIAISEAVEVAALNADTKYCLGSVLNHVLLHQTVIGEECLKQMEALGES
Sbjct: 241 LQVDPSSPGSLGIAISEAVEVAALNADTKYCLGSVLNHVLLHQTVIGEECLKQMEALGES 300

Query: 301 PDVIIGCTGGGSNFAGLSFPFLREKLAGKINPILRAVEPAACPSLTKGVYAYDFGDTAGM 360
           PDVIIGCTGGGSNFAGLSFPFLREKLAGKINPILRAVEPAACPSLTKGVYAYDFGDTAGM
Sbjct: 301 PDVIIGCTGGGSNFAGLSFPFLREKLAGKINPILRAVEPAACPSLTKGVYAYDFGDTAGM 360

Query: 361 TPLMKMHTLGHNFIPDPIHAGGLRYHGMAPLISHVYNLGLLEAISLPQTECFQGAIQFAR 420
           TPLMKMHTLGHNFIPDPIHAGGLRYHGMAPLISHVYNLGLLEAISLPQTECFQGAIQFAR
Sbjct: 361 TPLMKMHTLGHNFIPDPIHAGGLRYHGMAPLISHVYNLGLLEAISLPQTECFQGAIQFAR 420

Query: 421 SEGLIAAPEPTHAIAATIREALRCRETGESKVILTAMCGHGHFDLPAYEKYLQGGMTDLS 480
           SEGLIAAPEPTHAIAATIREALRCRETGESKVILTAMCGHGHFDLPAYEKYLQGGMTDLS
Sbjct: 421 SEGLIAAPEPTHAIAATIREALRCRETGESKVILTAMCGHGHFDLPAYEKYLQGGMTDLS 480

Query: 481 FAEEKIQESLTTIPQVA 498
           FAEEKIQESLTTIPQVA
Sbjct: 481 FAEEKIQESLTTIPQVA 497

BLAST of Moc04g37340 vs. ExPASy TrEMBL
Match: A0A0A0KUJ4 (Tryptophan synthase OS=Cucumis sativus OX=3659 GN=Csa_5G643330 PE=3 SV=1)

HSP 1 Score: 927.9 bits (2397), Expect = 1.7e-266
Identity = 450/497 (90.54%), Postives = 474/497 (95.37%), Query Frame = 0

Query: 1   MASHFLVAASSTSPAINGRVGEELPGRLSLKRNPRNLRFSNGYRTRAASNRNLKPVEVPR 60
           MA+ FL   S TS +IN  VGE+LPG LSLKR P +LRFSNGYRT+AASN NLK VEVPR
Sbjct: 1   MAATFLTVGSLTSSSIN-NVGEQLPGCLSLKRVPAHLRFSNGYRTKAASNPNLKSVEVPR 60

Query: 61  RWYNLVADLPVKPPPPLHPKTFQPIKPQDLSPLFPDALIEQEASTDRYINIPDEVVDIYG 120
           +WYNLVADLPVKPPPPLHPKT++PIKP+DLS LFPD LI+QEAST+RYINIPDEVVDIYG
Sbjct: 61  QWYNLVADLPVKPPPPLHPKTYEPIKPEDLSHLFPDELIKQEASTERYINIPDEVVDIYG 120

Query: 121 LWRPTPLIRAKRLEKLLDTPARIYYKYEGVSPAGSHKANSAVPQVWYNVQEGVKNVVTET 180
           LWRPTPLIRAKRLEKLLDTPARIYYKYEGVSPAGSHK NSAVPQVWYN QEGVKNVVTET
Sbjct: 121 LWRPTPLIRAKRLEKLLDTPARIYYKYEGVSPAGSHKPNSAVPQVWYNAQEGVKNVVTET 180

Query: 181 GAGQWGSALAFACSIFGLGCEVWQVRASYDQKPYRRLMMQTWGVKVHPSPSDLTDAGRKL 240
           GAGQWG +LAFACSIFG+GCEVWQVRASYDQKPYRR+MM+TWG KVHPSPSD+TDAGRK 
Sbjct: 181 GAGQWGCSLAFACSIFGIGCEVWQVRASYDQKPYRRMMMETWGAKVHPSPSDITDAGRKF 240

Query: 241 LQVDPSSPGSLGIAISEAVEVAALNADTKYCLGSVLNHVLLHQTVIGEECLKQMEALGES 300
           LQVDPSSPGSLGIAISEAVEVAALNADTKYCLGSVLNHVLLHQTVIGEECLKQ+EALGE+
Sbjct: 241 LQVDPSSPGSLGIAISEAVEVAALNADTKYCLGSVLNHVLLHQTVIGEECLKQLEALGET 300

Query: 301 PDVIIGCTGGGSNFAGLSFPFLREKLAGKINPILRAVEPAACPSLTKGVYAYDFGDTAGM 360
           PD+IIGCTGGGSNFAGLSFPFLREKLAG INP++RAVEPAACPSLTKGVYAYD+GDTAGM
Sbjct: 301 PDLIIGCTGGGSNFAGLSFPFLREKLAGNINPVIRAVEPAACPSLTKGVYAYDYGDTAGM 360

Query: 361 TPLMKMHTLGHNFIPDPIHAGGLRYHGMAPLISHVYNLGLLEAISLPQTECFQGAIQFAR 420
           TPLMKMHTLGHNFIPDPIHAGGLRYHGMAPLISHVYNLGLLEAISLPQTECFQGAIQFAR
Sbjct: 361 TPLMKMHTLGHNFIPDPIHAGGLRYHGMAPLISHVYNLGLLEAISLPQTECFQGAIQFAR 420

Query: 421 SEGLIAAPEPTHAIAATIREALRCRETGESKVILTAMCGHGHFDLPAYEKYLQGGMTDLS 480
           SEGLI APEPTHAIAATIREALRCRETGESKVILTAMCGHGHFDLPAYEKYLQGG++DLS
Sbjct: 421 SEGLIPAPEPTHAIAATIREALRCRETGESKVILTAMCGHGHFDLPAYEKYLQGGISDLS 480

Query: 481 FAEEKIQESLTTIPQVA 498
           FA EKIQESLTTIPQVA
Sbjct: 481 FANEKIQESLTTIPQVA 496

BLAST of Moc04g37340 vs. ExPASy TrEMBL
Match: A0A1S3CA62 (Tryptophan synthase OS=Cucumis melo OX=3656 GN=LOC103498574 PE=3 SV=1)

HSP 1 Score: 924.1 bits (2387), Expect = 2.5e-265
Identity = 446/497 (89.74%), Postives = 474/497 (95.37%), Query Frame = 0

Query: 1   MASHFLVAASSTSPAINGRVGEELPGRLSLKRNPRNLRFSNGYRTRAASNRNLKPVEVPR 60
           MA+ FL   S TS +IN  VGE+LPG LSLKR P +LRFSNGYRT++ASN NLK VE+PR
Sbjct: 1   MAATFLTVGSLTSSSIN-NVGEQLPGCLSLKRVPTHLRFSNGYRTKSASNPNLKAVEIPR 60

Query: 61  RWYNLVADLPVKPPPPLHPKTFQPIKPQDLSPLFPDALIEQEASTDRYINIPDEVVDIYG 120
           +WYNLVADLPVKPPPPLHPKT++PIKP+DLS LFPD LI+QEAST+RYINIPDEVVDIYG
Sbjct: 61  QWYNLVADLPVKPPPPLHPKTYEPIKPEDLSHLFPDELIKQEASTERYINIPDEVVDIYG 120

Query: 121 LWRPTPLIRAKRLEKLLDTPARIYYKYEGVSPAGSHKANSAVPQVWYNVQEGVKNVVTET 180
           LWRPTPLIRAKRLEKLLDTPARIYYKYEGVSPAGSHK NSAVPQVWYN QEGVKNVVTET
Sbjct: 121 LWRPTPLIRAKRLEKLLDTPARIYYKYEGVSPAGSHKPNSAVPQVWYNAQEGVKNVVTET 180

Query: 181 GAGQWGSALAFACSIFGLGCEVWQVRASYDQKPYRRLMMQTWGVKVHPSPSDLTDAGRKL 240
           GAGQWG +LAFACSIFG+GCEVWQVRASYDQKPYRR+MM+TWG KVHPSPSD+TDAGRK 
Sbjct: 181 GAGQWGCSLAFACSIFGIGCEVWQVRASYDQKPYRRMMMETWGAKVHPSPSDVTDAGRKF 240

Query: 241 LQVDPSSPGSLGIAISEAVEVAALNADTKYCLGSVLNHVLLHQTVIGEECLKQMEALGES 300
           LQVDPSSPGSLGIAISEAVEVAALNADTKYCLGSVLNHVLLHQTVIGEECLKQ+EALGES
Sbjct: 241 LQVDPSSPGSLGIAISEAVEVAALNADTKYCLGSVLNHVLLHQTVIGEECLKQLEALGES 300

Query: 301 PDVIIGCTGGGSNFAGLSFPFLREKLAGKINPILRAVEPAACPSLTKGVYAYDFGDTAGM 360
           PD+IIGCTGGGSNFAGLSFPFLREKLAG INP++RAVEPAACPSLTKGVYAYD+GDTAGM
Sbjct: 301 PDLIIGCTGGGSNFAGLSFPFLREKLAGNINPVIRAVEPAACPSLTKGVYAYDYGDTAGM 360

Query: 361 TPLMKMHTLGHNFIPDPIHAGGLRYHGMAPLISHVYNLGLLEAISLPQTECFQGAIQFAR 420
           TPLMKMHTLGHNFIPDPIHAGGLRYHGMAPLISHVYNLGLLEA+SLPQTECFQGAI+FAR
Sbjct: 361 TPLMKMHTLGHNFIPDPIHAGGLRYHGMAPLISHVYNLGLLEAVSLPQTECFQGAIKFAR 420

Query: 421 SEGLIAAPEPTHAIAATIREALRCRETGESKVILTAMCGHGHFDLPAYEKYLQGGMTDLS 480
           SEGLI APEPTHAIAATIREALRCRETGESKVILTAMCGHGHFDLPAYEKYLQGG+TDLS
Sbjct: 421 SEGLIPAPEPTHAIAATIREALRCRETGESKVILTAMCGHGHFDLPAYEKYLQGGITDLS 480

Query: 481 FAEEKIQESLTTIPQVA 498
           FA EK++ESLTTIPQVA
Sbjct: 481 FANEKMKESLTTIPQVA 496

BLAST of Moc04g37340 vs. ExPASy TrEMBL
Match: A0A6J1JY44 (Tryptophan synthase OS=Cucurbita maxima OX=3661 GN=LOC111489338 PE=3 SV=1)

HSP 1 Score: 904.4 bits (2336), Expect = 2.0e-259
Identity = 438/490 (89.39%), Postives = 466/490 (95.10%), Query Frame = 0

Query: 7   VAASSTSPAINGRVGEELPGRLSLKRNPRNLRFSNGYRTRAASNRNLKPVEVPRRWYNLV 66
           + ASS S  ING       G LS++R PR+LRFSNGYRT+AASN NLK VEVPR+WYNLV
Sbjct: 1   MVASSISSGING------GGCLSIERIPRHLRFSNGYRTKAASNPNLKAVEVPRQWYNLV 60

Query: 67  ADLPVKPPPPLHPKTFQPIKPQDLSPLFPDALIEQEASTDRYINIPDEVVDIYGLWRPTP 126
           ADLPVKPPPPLHPKTF+PIKP+DLSPLFPD LI+QEAS +RYI+IPDEVVDIYGLWRPTP
Sbjct: 61  ADLPVKPPPPLHPKTFEPIKPEDLSPLFPDELIKQEASAERYIDIPDEVVDIYGLWRPTP 120

Query: 127 LIRAKRLEKLLDTPARIYYKYEGVSPAGSHKANSAVPQVWYNVQEGVKNVVTETGAGQWG 186
           LIRAKRLEKLLDTPARIYYKYEGVSPAGSHK NSAVPQVWYNVQEGVKNVVTETGAGQWG
Sbjct: 121 LIRAKRLEKLLDTPARIYYKYEGVSPAGSHKPNSAVPQVWYNVQEGVKNVVTETGAGQWG 180

Query: 187 SALAFACSIFGLGCEVWQVRASYDQKPYRRLMMQTWGVKVHPSPSDLTDAGRKLLQVDPS 246
            +LAFACS+FG+GCEVWQVRASYDQKPYRR+MM+TWG KVHPSPS++TD+GRK LQVDPS
Sbjct: 181 CSLAFACSLFGIGCEVWQVRASYDQKPYRRMMMETWGAKVHPSPSNVTDSGRKFLQVDPS 240

Query: 247 SPGSLGIAISEAVEVAALNADTKYCLGSVLNHVLLHQTVIGEECLKQMEALGESPDVIIG 306
           SPGSLGIAISEAVEVAALNADTKYCLGSVLNHVLLHQTVIGEECLKQMEALGE+PDVIIG
Sbjct: 241 SPGSLGIAISEAVEVAALNADTKYCLGSVLNHVLLHQTVIGEECLKQMEALGETPDVIIG 300

Query: 307 CTGGGSNFAGLSFPFLREKLAGKINPILRAVEPAACPSLTKGVYAYDFGDTAGMTPLMKM 366
           CTGGGSNFAGLSFPFLREKLAGKI+P++RAVEPAACPSLTKGVYAYD+GDTAGMTPLMKM
Sbjct: 301 CTGGGSNFAGLSFPFLREKLAGKISPVIRAVEPAACPSLTKGVYAYDYGDTAGMTPLMKM 360

Query: 367 HTLGHNFIPDPIHAGGLRYHGMAPLISHVYNLGLLEAISLPQTECFQGAIQFARSEGLIA 426
           HTLGHNFIPDPIHAGGLRYHGMAPLISHVY+LGLLEA+SLPQTECFQGAIQFARSEGLI 
Sbjct: 361 HTLGHNFIPDPIHAGGLRYHGMAPLISHVYDLGLLEAVSLPQTECFQGAIQFARSEGLIP 420

Query: 427 APEPTHAIAATIREALRCRETGESKVILTAMCGHGHFDLPAYEKYLQGGMTDLSFAEEKI 486
           APEPTHAIA TIREALRCRETGESKVILTAMCGHGHFDL AYEKYLQGGMTDLSFAEEKI
Sbjct: 421 APEPTHAIAETIREALRCRETGESKVILTAMCGHGHFDLAAYEKYLQGGMTDLSFAEEKI 480

Query: 487 QESLTTIPQV 497
           +ESLTTIPQ+
Sbjct: 481 KESLTTIPQL 484

BLAST of Moc04g37340 vs. ExPASy TrEMBL
Match: A0A6J1FH74 (Tryptophan synthase OS=Cucurbita moschata OX=3662 GN=LOC111445435 PE=3 SV=1)

HSP 1 Score: 902.1 bits (2330), Expect = 1.0e-258
Identity = 438/490 (89.39%), Postives = 464/490 (94.69%), Query Frame = 0

Query: 7   VAASSTSPAINGRVGEELPGRLSLKRNPRNLRFSNGYRTRAASNRNLKPVEVPRRWYNLV 66
           + AS  S  ING       G LSL+R PR+LRFSNGYRT+AASN NLK VEVPR+WYNLV
Sbjct: 1   MVASLISSGING------GGCLSLERIPRHLRFSNGYRTKAASNPNLKAVEVPRQWYNLV 60

Query: 67  ADLPVKPPPPLHPKTFQPIKPQDLSPLFPDALIEQEASTDRYINIPDEVVDIYGLWRPTP 126
           ADLPVKPPPPLHPKTF+PIKP+DLSPLFPD LI+QEAS +RYI+IPDEVVDIYGLWRPTP
Sbjct: 61  ADLPVKPPPPLHPKTFEPIKPEDLSPLFPDELIKQEASAERYIDIPDEVVDIYGLWRPTP 120

Query: 127 LIRAKRLEKLLDTPARIYYKYEGVSPAGSHKANSAVPQVWYNVQEGVKNVVTETGAGQWG 186
           LIRAKRLEKLL TPARIYYKYEGVSPAGSHK NSAVPQVWYNVQEGVKNVVTETGAGQWG
Sbjct: 121 LIRAKRLEKLLGTPARIYYKYEGVSPAGSHKPNSAVPQVWYNVQEGVKNVVTETGAGQWG 180

Query: 187 SALAFACSIFGLGCEVWQVRASYDQKPYRRLMMQTWGVKVHPSPSDLTDAGRKLLQVDPS 246
            +LAFACS+FG+GCEVWQVRASYDQKPYRR+MM+TWG KVHPSPS++TD+GRK LQVDPS
Sbjct: 181 CSLAFACSLFGIGCEVWQVRASYDQKPYRRMMMETWGAKVHPSPSNVTDSGRKFLQVDPS 240

Query: 247 SPGSLGIAISEAVEVAALNADTKYCLGSVLNHVLLHQTVIGEECLKQMEALGESPDVIIG 306
           SPGSLGIAISEAVEVAALNADTKYCLGSVLNHVLLHQTVIGEECLKQMEALGE+PDVIIG
Sbjct: 241 SPGSLGIAISEAVEVAALNADTKYCLGSVLNHVLLHQTVIGEECLKQMEALGETPDVIIG 300

Query: 307 CTGGGSNFAGLSFPFLREKLAGKINPILRAVEPAACPSLTKGVYAYDFGDTAGMTPLMKM 366
           CTGGGSNFAGLSFPFLREKLAGKI+P++RAVEPAACPSLTKGVYAYD+GDTAGMTPLMKM
Sbjct: 301 CTGGGSNFAGLSFPFLREKLAGKISPVIRAVEPAACPSLTKGVYAYDYGDTAGMTPLMKM 360

Query: 367 HTLGHNFIPDPIHAGGLRYHGMAPLISHVYNLGLLEAISLPQTECFQGAIQFARSEGLIA 426
           HTLGHNFIPDPIHAGGLRYHGMAPLISHVYNLGLLEA+SLPQTECFQGAIQFARSEGLI 
Sbjct: 361 HTLGHNFIPDPIHAGGLRYHGMAPLISHVYNLGLLEAVSLPQTECFQGAIQFARSEGLIP 420

Query: 427 APEPTHAIAATIREALRCRETGESKVILTAMCGHGHFDLPAYEKYLQGGMTDLSFAEEKI 486
           APEPTHAIA TIREALRCRETGESKVILTAMCGHGHFDL AYEKYLQGGMTDLSFAEEKI
Sbjct: 421 APEPTHAIAETIREALRCRETGESKVILTAMCGHGHFDLAAYEKYLQGGMTDLSFAEEKI 480

Query: 487 QESLTTIPQV 497
           +ESLTTIPQ+
Sbjct: 481 KESLTTIPQL 484

BLAST of Moc04g37340 vs. TAIR 10
Match: AT5G38530.1 (tryptophan synthase beta type 2 )

HSP 1 Score: 771.9 bits (1992), Expect = 3.1e-223
Identity = 370/474 (78.06%), Postives = 422/474 (89.03%), Query Frame = 0

Query: 28  LSLKRNPRN-LRFSNG--YRTRAA--SNRNLKPVEVPRRWYNLVADLPVKPPPPLHPKTF 87
           L+LKR      R SNG   R +AA  S  N   VE+P++WYNLVADL VKPPPPLHPKTF
Sbjct: 32  LTLKRKSNQATRVSNGSSLRVKAALRSTHNKSVVEIPKQWYNLVADLSVKPPPPLHPKTF 91

Query: 88  QPIKPQDLSPLFPDALIEQEASTDRYINIPDEVVDIYGLWRPTPLIRAKRLEKLLDTPAR 147
           +PIKP+DL+ LFP+ LI+QEA+ +R+I+IP+EV++IY LWRPTPLIRAKRLEKLL TPAR
Sbjct: 92  EPIKPEDLAHLFPNELIKQEATQERFIDIPEEVLEIYKLWRPTPLIRAKRLEKLLQTPAR 151

Query: 148 IYYKYEGVSPAGSHKANSAVPQVWYNVQEGVKNVVTETGAGQWGSALAFACSIFGLGCEV 207
           IY+KYEG SPAGSHK N+AVPQ +YN +EGVKNVVTETGAGQWGS+LAFA S+FGL CEV
Sbjct: 152 IYFKYEGGSPAGSHKPNTAVPQAYYNAKEGVKNVVTETGAGQWGSSLAFASSLFGLDCEV 211

Query: 208 WQVRASYDQKPYRRLMMQTWGVKVHPSPSDLTDAGRKLLQVDPSSPGSLGIAISEAVEVA 267
           WQV  SY  KPYRRLMMQTWG KVHPSPSDLT+AGR++L+ DPSSPGSLGIAISEAVEVA
Sbjct: 212 WQVANSYHTKPYRRLMMQTWGAKVHPSPSDLTEAGRRILESDPSSPGSLGIAISEAVEVA 271

Query: 268 ALNADTKYCLGSVLNHVLLHQTVIGEECLKQMEALGESPDVIIGCTGGGSNFAGLSFPFL 327
           A N DTKYCLGSVLNHVLLHQT+IGEEC++QME  GE+PD+IIGCTGGGSNFAGLSFPF+
Sbjct: 272 ARNEDTKYCLGSVLNHVLLHQTIIGEECIQQMENFGETPDLIIGCTGGGSNFAGLSFPFI 331

Query: 328 REKLAGKINPILRAVEPAACPSLTKGVYAYDFGDTAGMTPLMKMHTLGHNFIPDPIHAGG 387
           REKL GKINP++RAVEP+ACPSLTKGVYAYDFGDTAG+TPLMKMHTLGH+FIPDPIHAGG
Sbjct: 332 REKLKGKINPVIRAVEPSACPSLTKGVYAYDFGDTAGLTPLMKMHTLGHDFIPDPIHAGG 391

Query: 388 LRYHGMAPLISHVYNLGLLEAISLPQTECFQGAIQFARSEGLIAAPEPTHAIAATIREAL 447
           LRYHGMAPLISHVY  G +EAIS+PQ ECFQGAIQFAR+EG+I APEPTHAIAATIREAL
Sbjct: 392 LRYHGMAPLISHVYEQGFMEAISIPQIECFQGAIQFARTEGIIPAPEPTHAIAATIREAL 451

Query: 448 RCRETGESKVILTAMCGHGHFDLPAYEKYLQGGMTDLSFAEEKIQESLTTIPQV 497
           RC+ETGE+KVIL AMCGHGHFDL +Y+KYL+G + DLSF+EEKI+ESL+ +P V
Sbjct: 452 RCKETGEAKVILMAMCGHGHFDLTSYDKYLKGELVDLSFSEEKIRESLSKVPHV 505

BLAST of Moc04g37340 vs. TAIR 10
Match: AT5G54810.1 (tryptophan synthase beta-subunit 1 )

HSP 1 Score: 119.4 bits (298), Expect = 8.2e-27
Identity = 106/349 (30.37%), Postives = 147/349 (42.12%), Query Frame = 0

Query: 143 IYYKYEGVSPAGSHKANSAVPQVWYNVQEGVKNVVTETGAGQWGSALAFACSIFGLGCEV 202
           IY K E ++  G+HK N+AV Q     + G K ++ ETGAGQ G A A  C+ FGL C +
Sbjct: 151 IYLKREDLNHTGAHKINNAVAQALLAKRLGKKRIIAETGAGQHGVATATVCARFGLECII 210

Query: 203 WQVRASYDQKPYRRLMMQTWGVK---VHPSPSDLTDAGRKLLQVDPSSPGSLGIAISEAV 262
           +      +++      M+  G +   VH   + L D                  A SEA+
Sbjct: 211 YMGAQDMERQALNVFRMRLLGAEVRGVHSGTATLKD------------------ATSEAI 270

Query: 263 EVAALNAD-TKYCLGSVLN------HVLLHQTVIGEECLKQ-MEALGESPDVIIGCTGGG 322
                N + T Y LGSV         V     VIG+E  KQ +E  G  PDV++ C GGG
Sbjct: 271 RDWVTNVETTHYILGSVAGPHPYPMMVRDFHAVIGKETRKQALEKWGGKPDVLVACVGGG 330

Query: 323 SNFAGLSFPFLREKLAGKINPILRAVEPAA--------CPSLTKGVYAYDFGDTAGMTPL 382
           SN  GL   F+ +     I      VE A           +LTKG    D G   G    
Sbjct: 331 SNAMGLFHEFVNDTEVRMI-----GVEAAGFGLDSGKHAATLTKG----DVGVLHGAMSY 390

Query: 383 MKMHTLGHNFIPDPIHAGGLRYHGMAPLISHVYNLGLLEAISLPQTECFQGAIQFARSEG 442
           +     G    P  I A GL Y G+ P  S   ++G  E  S+   E  +   + +R EG
Sbjct: 391 LLQDDDGQIIEPHSISA-GLDYPGVGPEHSFFKDMGRAEYYSITDEEALEAFKRVSRLEG 450

Query: 443 LIAAPEPTHAIAATIREALRCRETGESKVILTAMCGHGHFDLPAYEKYL 473
           +I A E +HA+A   +    C    +   ++    G G  D+    KYL
Sbjct: 451 IIPALETSHALAYLEK---LCPTLSDGTRVVLNFSGRGDKDVQTVAKYL 468

BLAST of Moc04g37340 vs. TAIR 10
Match: AT4G27070.1 (tryptophan synthase beta-subunit 2 )

HSP 1 Score: 119.0 bits (297), Expect = 1.1e-26
Identity = 107/350 (30.57%), Postives = 149/350 (42.57%), Query Frame = 0

Query: 143 IYYKYEGVSPAGSHKANSAVPQVWYNVQEGVKNVVTETGAGQWGSALAFACSIFGLGCEV 202
           IY K E ++  G+HK N+AV Q     + G K ++ ETGAGQ G A A  C+ FGL C +
Sbjct: 156 IYLKREDLNHTGAHKINNAVAQALLAKRLGKKRIIAETGAGQHGVATATVCARFGLQCII 215

Query: 203 WQVRASYDQKPYRRLMMQTWGVK---VHPSPSDLTDAGRKLLQVDPSSPGSLGIAISEAV 262
           +      +++      M+  G +   VH   + L D                  A SEA+
Sbjct: 216 YMGAQDMERQALNVFRMRLLGAEVRGVHSGTATLKD------------------ATSEAI 275

Query: 263 EVAALNAD-TKYCLGSVLN------HVLLHQTVIGEECLKQ-MEALGESPDVIIGCTGGG 322
                N + T Y LGSV         V     VIG+E  KQ ME  G  PDV++ C GGG
Sbjct: 276 RDWVTNVETTHYILGSVAGPHPYPMMVRDFHAVIGKETRKQAMEKWGGKPDVLVACVGGG 335

Query: 323 SNFAGLSFPFLREKLAGKINPILRAVEPAA--------CPSLTKGVYAYDFGDTAGMTPL 382
           SN  GL   F+ +     I      VE A           +LTKG    D G   G    
Sbjct: 336 SNAMGLFHEFVDDTEVRMI-----GVEAAGFGLDSGKHAATLTKG----DVGVLHGAMSY 395

Query: 383 MKMHTLGHNFIPDPIHAGGLRYHGMAPLISHVYNLGLLEAISLPQTECFQGAIQFARSEG 442
           +     G    P  I A GL Y G+ P  S + ++G  E  S+   E  +   + +R EG
Sbjct: 396 LLQDDDGQIIEPHSISA-GLDYPGVGPEHSFLKDVGRAEYFSVTDEEALEAFKRVSRLEG 455

Query: 443 LIAAPEPTHAIAATIREALRCRETGESKVILTAMCGHGHFDLPAYEKYLQ 474
           +I A E +HA+A   +    C    +   ++    G G  D+    KYL+
Sbjct: 456 IIPALETSHALAHLEK---LCPTLPDGARVVLNFSGRGDKDVQTAIKYLE 474

BLAST of Moc04g37340 vs. TAIR 10
Match: AT5G28237.1 (Pyridoxal-5'-phosphate-dependent enzyme family protein )

HSP 1 Score: 115.2 bits (287), Expect = 1.6e-25
Identity = 116/374 (31.02%), Postives = 165/374 (44.12%), Query Frame = 0

Query: 123 RPTPLIRAKRLEK-------LLDTPARIYYKYEGVSPAGSHKANSAVPQVWYNVQEGVKN 182
           R TPL  A+RL +        ++    IY K E +S  GSHK N+A+ Q   + + G   
Sbjct: 118 RETPLYFAERLTEHYKNIVPTIEGGPEIYLKREDLSHCGSHKINNALAQAMISRRLGCSR 177

Query: 183 VVTETGAGQWGSALAFACSIFGLGCEVWQVRASYDQKPYRRLMMQTWGVKVHPSPSDLTD 242
           VV  TGAGQ G A A AC+   L C V+   A  +++ +  L M+  G +V         
Sbjct: 178 VVAATGAGQHGVATAAACAKLSLECTVFMGAADIEKQSFNVLSMKLLGAQV--------- 237

Query: 243 AGRKLLQVDPSSPGSLGIAISEAVEVAALNADTKYCL-GSVLNH------VLLHQTVIGE 302
                     S  G+   A SEA+     N  T Y L G+V+        V   Q+VIG+
Sbjct: 238 ---------ISVEGTFKDASSEAIRNWVENLYTTYYLSGTVVGPHPCPIIVREFQSVIGK 297

Query: 303 ECLKQMEAL-GESPDVIIGCTGGGSNFAGLSFPFLREKLAGKINPILRAVEPAACPSLTK 362
           E  +Q + L G  PDV++ C G GSN  GL   F+     G  +  L  VE AA   L  
Sbjct: 298 ETRRQAKQLWGGKPDVLVACVGSGSNALGLFHEFV-----GDEDVRLVGVE-AAGLGLDS 357

Query: 363 GVYA--YDFGDTA---GMTPLMKMHTLGHNFIPDPIHAGGLRYHGMAPLISHVYNLGLLE 422
           G ++    FGD     G    +     G    P  +   GL Y G+ P IS +   G  E
Sbjct: 358 GKHSATLAFGDVGVYHGSMSYLLQDDQGQILKPHSVGV-GLEYPGVGPEISFMKETGRAE 417

Query: 423 AISLPQTECFQGAIQFARSEGLIAAPEPTHAIAATIREALRCRETGESKVILTAMCGHGH 477
             +    E  Q  ++ +R EG+I A E +HA+A   +     R+   +KV++    G G 
Sbjct: 418 FYTATDEEAIQACMRLSRLEGIIPALEASHALAFLDKLVPTLRD--GAKVVVNC-SGRGD 460

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022134316.11.1e-291100.00uncharacterized protein LOC111006607 [Momordica charantia][more]
XP_038890851.19.4e-26791.35tryptophan synthase beta chain 2 [Benincasa hispida][more]
XP_031741291.13.6e-26690.54uncharacterized protein LOC101219640 [Cucumis sativus][more]
XP_008459440.15.2e-26589.74PREDICTED: tryptophan synthase beta chain 2-like [Cucumis melo][more]
XP_022993275.14.2e-25989.39uncharacterized protein LOC111489338 [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
O674092.2e-15461.88Tryptophan synthase beta chain 2 OS=Aquifex aeolicus (strain VF5) OX=224324 GN=t... [more]
Q8TL441.0e-15159.81Tryptophan synthase beta chain 2 OS=Methanosarcina acetivorans (strain ATCC 3539... [more]
Q8Q0011.4e-15160.53Tryptophan synthase beta chain 2 OS=Methanosarcina mazei (strain ATCC BAA-159 / ... [more]
O290288.8e-15159.72Tryptophan synthase beta chain 2 OS=Archaeoglobus fulgidus (strain ATCC 49558 / ... [more]
Q9WZ096.1e-14460.67Tryptophan synthase beta chain 2 OS=Thermotoga maritima (strain ATCC 43589 / DSM... [more]
Match NameE-valueIdentityDescription
A0A6J1BXI75.3e-292100.00Tryptophan synthase OS=Momordica charantia OX=3673 GN=LOC111006607 PE=3 SV=1[more]
A0A0A0KUJ41.7e-26690.54Tryptophan synthase OS=Cucumis sativus OX=3659 GN=Csa_5G643330 PE=3 SV=1[more]
A0A1S3CA622.5e-26589.74Tryptophan synthase OS=Cucumis melo OX=3656 GN=LOC103498574 PE=3 SV=1[more]
A0A6J1JY442.0e-25989.39Tryptophan synthase OS=Cucurbita maxima OX=3661 GN=LOC111489338 PE=3 SV=1[more]
A0A6J1FH741.0e-25889.39Tryptophan synthase OS=Cucurbita moschata OX=3662 GN=LOC111445435 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT5G38530.13.1e-22378.06tryptophan synthase beta type 2 [more]
AT5G54810.18.2e-2730.37tryptophan synthase beta-subunit 1 [more]
AT4G27070.11.1e-2630.57tryptophan synthase beta-subunit 2 [more]
AT5G28237.11.6e-2531.02Pyridoxal-5'-phosphate-dependent enzyme family protein [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (OHB3-1) v2
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR006316Tryptophan synthase, beta chain-likePIRSFPIRSF500824TrpB_prokcoord: 47..481
e-value: 1.5E-194
score: 644.5
IPR006316Tryptophan synthase, beta chain-likeTIGRFAMTIGR01415TIGR01415coord: 58..474
e-value: 1.2E-189
score: 628.3
IPR023026Tryptophan synthase beta chain/beta chain-likePIRSFPIRSF001413Trp_syn_betacoord: 54..478
e-value: 2.3E-113
score: 377.4
IPR023026Tryptophan synthase beta chain/beta chain-likeHAMAPMF_00133Trp_synth_betacoord: 66..472
score: 38.115257
IPR036052Tryptophan synthase beta subunit-like PLP-dependent enzymeGENE3D3.40.50.1100coord: 74..284
e-value: 4.2E-147
score: 492.1
IPR036052Tryptophan synthase beta subunit-like PLP-dependent enzymeGENE3D3.40.50.1100coord: 123..465
e-value: 4.2E-147
score: 492.1
IPR036052Tryptophan synthase beta subunit-like PLP-dependent enzymeSUPERFAMILY53686Tryptophan synthase beta subunit-like PLP-dependent enzymescoord: 74..471
IPR001926Pyridoxal-phosphate dependent enzymePFAMPF00291PALPcoord: 121..459
e-value: 5.1E-40
score: 137.7
NoneNo IPR availablePANTHERPTHR48077:SF7BNAA04G27890D PROTEINcoord: 1..495
NoneNo IPR availablePANTHERPTHR48077TRYPTOPHAN SYNTHASE-RELATEDcoord: 1..495
IPR006653Tryptophan synthase, beta chain, conserved sitePROSITEPS00168TRP_SYNTHASE_BETAcoord: 150..164
IPR006654Tryptophan synthase, beta chainCDDcd06446Trp-synth_Bcoord: 94..468
e-value: 2.14393E-173
score: 490.51

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Moc04g37340.1Moc04g37340.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0000162 tryptophan biosynthetic process
biological_process GO:0006568 tryptophan metabolic process
molecular_function GO:0052684 L-serine hydro-lyase (adding indole, L-tryptophan-forming) activity
molecular_function GO:0030170 pyridoxal phosphate binding
molecular_function GO:0004834 tryptophan synthase activity