Moc04g37060 (gene) Bitter gourd (OHB3-1) v2

Overview


Name	Moc04g37060
Type	gene
Organism	Momordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
Description	Cytochrome P450
Location	chr4: 27766520 .. 27769195 (+)
RNA-Seq Expression	Moc04g37060
Synteny	Moc04g37060

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCGGTGGCCATGGCGATTCTGGCACTGATCTTGCTCCTCTGTTTCTGCTCTGCTCTGCTGCGATGGAATGAAGTTAGATTCAGCAAAAAAGGGCTCCCCCCTGGTACAATGGGCTGGCCTGTTTTTGGAGAAACCACTGAGTTTCTTAAACAAGGCCCCAACTTCATGAAGACCCAGAGAGCAAGGTTCCTCTTTTTTCCATCTCTCTATTTCCTCTCTCTCTCTCTCTCTAGATTTCTAACTGTTGGTTGCTCTGTTTTTTCTTTAAGGTATGGGAGCATTTTCAAATCCCATATTTTGGGGTGTCCTACTGTTGTTTCCATGGATCCAGAGGTCAATCGATATGTTCTGATGAATGAATCTAAAGGCCTTGTCCCTGGCTACCCTCAGTCCATGCTTGACATCCTCGGAAAATGTAATATCGCCGCCGTCCACGGCGGCGCCCACAAGCTCATGAGAGCTGCTCTGCTCGCCATCGTCTCCCCGGCCATGATTAAAGACCGTCTTCTACCTAAAGTTGATGACTTCATGCGGTCCCATTTGAGGAATTGGGATCGCCAAATCATTGACATCCAAGACAAAACTAAAGAAGTAAACTTTCTTGTGACGATAAATTTTTTGATTTTGTTCTGATACTCTGTATTCCTCTGTTTTTCACTCATTGAATCAATCCCTGTTTGTCTGTCTTGCAGATGGCTCTCCGGTCGTCTTTGAAGCAGATTGCGGGAATCGAGTCCGGCCCGTTATCGGAATCGTTCATTCCGGAGTTCTTCAAGCTTGTTCTTGGCACTCTCTCGCTCCCCATCGATCTTCCCGGCACCAACTACCGCCGAGGAATTCAGGTAGTTTGGATTTAGTAGTGATTTAACATCGCATCCGAACGAAAAGTACTATGTTCGAACTCCAATAACGTCGAAGGGAGAGCGTCGAGAAAATATGAATAAATGATTAAATATACCATAACGGTAACGGATTTGAGTGAGAATAACAGAGAGGCTTGTGATTTCAGGCAAGGAAGAACATCATAAAGCTGTTGCAGCAGCTGATAAACGAGAGAAGAGCCTCAAAGACAGAGAAAGACGACATGCTTGGGTTTTTTATGAGTGAGGAGAACAAATATAAGCTTAAAGATGAAGAGATTATTGATCAGATCATCACTGTCTTATACTCTGGCTATGAAACTGTTTCCACAACTTCTATGATGGCTGTCAAGTTCCTCCATGATCATCCCAAAGTTCTTCAGCAACTCAGAGTGAGTTGAAAAGAAACTGAAAGATTGAAACTTTCAAGTTCTAAAGTGCTGTTGGGATTTTTTTCCCCCTTTCTAATTAGAGATTTGAATGAAAACTGCAGGAAGAGCATATGGCTATCAGAGAGAGGAAAAATCCAGAGGACCCCATTGATTGGGATGATCTGAAAGCCATGAAGTTCACTCGTGCGGTAAGATTTTCATGTTCTTTTGCTGTCGTCTTCCTAACCGTTCTTTGAGGAGGGTAAATATAAAGACTAACTTTGATCTGTTTTTAACTGTTTAGGTGATATTTGAAACATCAAGACTGGCCACAATTGTTAATGGGGTTTTGAGGAAAACTACAAAGGACATGGAACTTAATGGTAAGTTTCATTAAAATAATGAAAATCATAAAAAAATGATAGAAATGAACTTTATAATGCATAAAAATGAGAACAAAATTTGGTTGCCCACATGCCTTTGGGGCAGGACAATATTTTTCTCTCTTTTCGAACTTTTCTTCACAAAGTAATGGGCATGGCTGCTGGTTTTTGAGATAAAAAATGGCTTAGTTTCACAATATTTTGGCCCTTTTATAGTTCACCGGATAAGTCATATATCTTCGATTAAAAAGTAAGATGTAAAAAGTAAGATGTTTGAATATCACCGGTCACACAACTTTTGTTTTGTGATATTTAGGGCTTCAAGATCCTCTTTTTGCAGTTACCAATTCATCTCCCTTTTCACTTTGGGCCACTCTCTCTCTCTCTTCTATGTTCTGTTGTGGGTCAGGCTTAGATAAGGCTCATAAAGAATGACAGAACTTCTTCACCTCCTCATTACAAAAATGTTGTGAGATCAACCCCAAAAAGTTGGGCTAAAAAGTCTCAATCTTTAACCTTTATTTTGGTTTGCTTTTACAGGCTTTCTCATTCCAAAAGGATGGAGGATTTATGTCTACACCAGAGAGATAAACTATGACCCTTTTCTCTATTCTGAACCATTCACCTTCAACCCTTGGAGATGGCTGGTGGGTCTTTTTTTATTTATTTTTTATTTTTCGCTCTCTCTCTTACCCTCTATTGCAAAATGACAACTCTGCCCTTGTATGACATTTGCAGGACAAAAACCTGGAGTCCCATAATTACTTCTTCATCTTTGGAGGTGGCAGCAGACTTTGCCCTGGGAAAGAACTGGGAGTTGCAGAGATTTCTACTTTTCTTCATTACTTTGTAACTAAATACAGGTAGCTTTCTTTTTCTTATCCATTGTTTGGTTGCTCAGAAACAGCAAGAAAATCAAAAACAGGAAGCTGAGATCAAGAGGGAACCTTTTTTTTCTTTTTGTCTTGCAGGTGGGAAGAAGTTGGAGGAGATGAGCTGTTGAAATTTCCAAGAGTGGAAGCCCCAAGTGGGTTCCACATTAGGGTTTCATCTTACTGA

mRNA sequence

ATGGCGGTGGCCATGGCGATTCTGGCACTGATCTTGCTCCTCTGTTTCTGCTCTGCTCTGCTGCGATGGAATGAAGTTAGATTCAGCAAAAAAGGGCTCCCCCCTGGTACAATGGGCTGGCCTGTTTTTGGAGAAACCACTGAGTTTCTTAAACAAGGCCCCAACTTCATGAAGACCCAGAGAGCAAGGTATGGGAGCATTTTCAAATCCCATATTTTGGGGTGTCCTACTGTTGTTTCCATGGATCCAGAGGTCAATCGATATGTTCTGATGAATGAATCTAAAGGCCTTGTCCCTGGCTACCCTCAGTCCATGCTTGACATCCTCGGAAAATGTAATATCGCCGCCGTCCACGGCGGCGCCCACAAGCTCATGAGAGCTGCTCTGCTCGCCATCGTCTCCCCGGCCATGATTAAAGACCGTCTTCTACCTAAAGTTGATGACTTCATGCGGTCCCATTTGAGGAATTGGGATCGCCAAATCATTGACATCCAAGACAAAACTAAAGAAATGGCTCTCCGGTCGTCTTTGAAGCAGATTGCGGGAATCGAGTCCGGCCCGTTATCGGAATCGTTCATTCCGGAGTTCTTCAAGCTTGTTCTTGGCACTCTCTCGCTCCCCATCGATCTTCCCGGCACCAACTACCGCCGAGGAATTCAGGCAAGGAAGAACATCATAAAGCTGTTGCAGCAGCTGATAAACGAGAGAAGAGCCTCAAAGACAGAGAAAGACGACATGCTTGGGTTTTTTATGAGTGAGGAGAACAAATATAAGCTTAAAGATGAAGAGATTATTGATCAGATCATCACTGTCTTATACTCTGGCTATGAAACTGTTTCCACAACTTCTATGATGGCTGTCAAGTTCCTCCATGATCATCCCAAAGTTCTTCAGCAACTCAGAGAAGAGCATATGGCTATCAGAGAGAGGAAAAATCCAGAGGACCCCATTGATTGGGATGATCTGAAAGCCATGAAGTTCACTCGTGCGGTGATATTTGAAACATCAAGACTGGCCACAATTGTTAATGGGGTTTTGAGGAAAACTACAAAGGACATGGAACTTAATGGCTTTCTCATTCCAAAAGGATGGAGGATTTATGTCTACACCAGAGAGATAAACTATGACCCTTTTCTCTATTCTGAACCATTCACCTTCAACCCTTGGAGATGGCTGGACAAAAACCTGGAGTCCCATAATTACTTCTTCATCTTTGGAGGTGGCAGCAGACTTTGCCCTGGGAAAGAACTGGGAGTTGCAGAGATTTCTACTTTTCTTCATTACTTTGTAACTAAATACAGGTGGGAAGAAGTTGGAGGAGATGAGCTGTTGAAATTTCCAAGAGTGGAAGCCCCAAGTGGGTTCCACATTAGGGTTTCATCTTACTGA

Coding sequence (CDS)

ATGGCGGTGGCCATGGCGATTCTGGCACTGATCTTGCTCCTCTGTTTCTGCTCTGCTCTGCTGCGATGGAATGAAGTTAGATTCAGCAAAAAAGGGCTCCCCCCTGGTACAATGGGCTGGCCTGTTTTTGGAGAAACCACTGAGTTTCTTAAACAAGGCCCCAACTTCATGAAGACCCAGAGAGCAAGGTATGGGAGCATTTTCAAATCCCATATTTTGGGGTGTCCTACTGTTGTTTCCATGGATCCAGAGGTCAATCGATATGTTCTGATGAATGAATCTAAAGGCCTTGTCCCTGGCTACCCTCAGTCCATGCTTGACATCCTCGGAAAATGTAATATCGCCGCCGTCCACGGCGGCGCCCACAAGCTCATGAGAGCTGCTCTGCTCGCCATCGTCTCCCCGGCCATGATTAAAGACCGTCTTCTACCTAAAGTTGATGACTTCATGCGGTCCCATTTGAGGAATTGGGATCGCCAAATCATTGACATCCAAGACAAAACTAAAGAAATGGCTCTCCGGTCGTCTTTGAAGCAGATTGCGGGAATCGAGTCCGGCCCGTTATCGGAATCGTTCATTCCGGAGTTCTTCAAGCTTGTTCTTGGCACTCTCTCGCTCCCCATCGATCTTCCCGGCACCAACTACCGCCGAGGAATTCAGGCAAGGAAGAACATCATAAAGCTGTTGCAGCAGCTGATAAACGAGAGAAGAGCCTCAAAGACAGAGAAAGACGACATGCTTGGGTTTTTTATGAGTGAGGAGAACAAATATAAGCTTAAAGATGAAGAGATTATTGATCAGATCATCACTGTCTTATACTCTGGCTATGAAACTGTTTCCACAACTTCTATGATGGCTGTCAAGTTCCTCCATGATCATCCCAAAGTTCTTCAGCAACTCAGAGAAGAGCATATGGCTATCAGAGAGAGGAAAAATCCAGAGGACCCCATTGATTGGGATGATCTGAAAGCCATGAAGTTCACTCGTGCGGTGATATTTGAAACATCAAGACTGGCCACAATTGTTAATGGGGTTTTGAGGAAAACTACAAAGGACATGGAACTTAATGGCTTTCTCATTCCAAAAGGATGGAGGATTTATGTCTACACCAGAGAGATAAACTATGACCCTTTTCTCTATTCTGAACCATTCACCTTCAACCCTTGGAGATGGCTGGACAAAAACCTGGAGTCCCATAATTACTTCTTCATCTTTGGAGGTGGCAGCAGACTTTGCCCTGGGAAAGAACTGGGAGTTGCAGAGATTTCTACTTTTCTTCATTACTTTGTAACTAAATACAGGTGGGAAGAAGTTGGAGGAGATGAGCTGTTGAAATTTCCAAGAGTGGAAGCCCCAAGTGGGTTCCACATTAGGGTTTCATCTTACTGA

Protein sequence

MAVAMAILALILLLCFCSALLRWNEVRFSKKGLPPGTMGWPVFGETTEFLKQGPNFMKTQRARYGSIFKSHILGCPTVVSMDPEVNRYVLMNESKGLVPGYPQSMLDILGKCNIAAVHGGAHKLMRAALLAIVSPAMIKDRLLPKVDDFMRSHLRNWDRQIIDIQDKTKEMALRSSLKQIAGIESGPLSESFIPEFFKLVLGTLSLPIDLPGTNYRRGIQARKNIIKLLQQLINERRASKTEKDDMLGFFMSEENKYKLKDEEIIDQIITVLYSGYETVSTTSMMAVKFLHDHPKVLQQLREEHMAIRERKNPEDPIDWDDLKAMKFTRAVIFETSRLATIVNGVLRKTTKDMELNGFLIPKGWRIYVYTREINYDPFLYSEPFTFNPWRWLDKNLESHNYFFIFGGGSRLCPGKELGVAEISTFLHYFVTKYRWEEVGGDELLKFPRVEAPSGFHIRVSSY

Homology

[Close]

BLAST of Moc04g37060 vs. NCBI nr
Match: XP_022134388.1 (cytochrome P450 85A-like [Momordica charantia])

HSP 1 Score: 937.2 bits (2421), Expect = 5.5e-269
Identity = 462/462 (100.00%), Postives = 462/462 (100.00%), Query Frame = 0


Query: 1   MAVAMAILALILLLCFCSALLRWNEVRFSKKGLPPGTMGWPVFGETTEFLKQGPNFMKTQ 60
           MAVAMAILALILLLCFCSALLRWNEVRFSKKGLPPGTMGWPVFGETTEFLKQGPNFMKTQ
Sbjct: 1   MAVAMAILALILLLCFCSALLRWNEVRFSKKGLPPGTMGWPVFGETTEFLKQGPNFMKTQ 60

Query: 61  RARYGSIFKSHILGCPTVVSMDPEVNRYVLMNESKGLVPGYPQSMLDILGKCNIAAVHGG 120
           RARYGSIFKSHILGCPTVVSMDPEVNRYVLMNESKGLVPGYPQSMLDILGKCNIAAVHGG
Sbjct: 61  RARYGSIFKSHILGCPTVVSMDPEVNRYVLMNESKGLVPGYPQSMLDILGKCNIAAVHGG 120

Query: 121 AHKLMRAALLAIVSPAMIKDRLLPKVDDFMRSHLRNWDRQIIDIQDKTKEMALRSSLKQI 180
           AHKLMRAALLAIVSPAMIKDRLLPKVDDFMRSHLRNWDRQIIDIQDKTKEMALRSSLKQI
Sbjct: 121 AHKLMRAALLAIVSPAMIKDRLLPKVDDFMRSHLRNWDRQIIDIQDKTKEMALRSSLKQI 180

Query: 181 AGIESGPLSESFIPEFFKLVLGTLSLPIDLPGTNYRRGIQARKNIIKLLQQLINERRASK 240
           AGIESGPLSESFIPEFFKLVLGTLSLPIDLPGTNYRRGIQARKNIIKLLQQLINERRASK
Sbjct: 181 AGIESGPLSESFIPEFFKLVLGTLSLPIDLPGTNYRRGIQARKNIIKLLQQLINERRASK 240

Query: 241 TEKDDMLGFFMSEENKYKLKDEEIIDQIITVLYSGYETVSTTSMMAVKFLHDHPKVLQQL 300
           TEKDDMLGFFMSEENKYKLKDEEIIDQIITVLYSGYETVSTTSMMAVKFLHDHPKVLQQL
Sbjct: 241 TEKDDMLGFFMSEENKYKLKDEEIIDQIITVLYSGYETVSTTSMMAVKFLHDHPKVLQQL 300

Query: 301 REEHMAIRERKNPEDPIDWDDLKAMKFTRAVIFETSRLATIVNGVLRKTTKDMELNGFLI 360
           REEHMAIRERKNPEDPIDWDDLKAMKFTRAVIFETSRLATIVNGVLRKTTKDMELNGFLI
Sbjct: 301 REEHMAIRERKNPEDPIDWDDLKAMKFTRAVIFETSRLATIVNGVLRKTTKDMELNGFLI 360

Query: 361 PKGWRIYVYTREINYDPFLYSEPFTFNPWRWLDKNLESHNYFFIFGGGSRLCPGKELGVA 420
           PKGWRIYVYTREINYDPFLYSEPFTFNPWRWLDKNLESHNYFFIFGGGSRLCPGKELGVA
Sbjct: 361 PKGWRIYVYTREINYDPFLYSEPFTFNPWRWLDKNLESHNYFFIFGGGSRLCPGKELGVA 420

Query: 421 EISTFLHYFVTKYRWEEVGGDELLKFPRVEAPSGFHIRVSSY 463
           EISTFLHYFVTKYRWEEVGGDELLKFPRVEAPSGFHIRVSSY
Sbjct: 421 EISTFLHYFVTKYRWEEVGGDELLKFPRVEAPSGFHIRVSSY 462

[Close]

BLAST of Moc04g37060 vs. NCBI nr
Match: XP_038890036.1 (cytochrome P450 85A-like [Benincasa hispida])

HSP 1 Score: 904.8 bits (2337), Expect = 3.0e-259
Identity = 441/462 (95.45%), Postives = 452/462 (97.84%), Query Frame = 0


Query: 1   MAVAMAILALILLLCFCSALLRWNEVRFSKKGLPPGTMGWPVFGETTEFLKQGPNFMKTQ 60
           MA+AM ILAL LLLCFCSALLRWNEVR+SKKGLPPGTMGWPVFGETTEFLKQGPNFMK+Q
Sbjct: 1   MALAMVILALFLLLCFCSALLRWNEVRYSKKGLPPGTMGWPVFGETTEFLKQGPNFMKSQ 60

Query: 61  RARYGSIFKSHILGCPTVVSMDPEVNRYVLMNESKGLVPGYPQSMLDILGKCNIAAVHGG 120
           RARYGSIFKSHILGCPT+VSMDPEVNRYVLMNESKGLVPGYPQSMLDILGKCNIAAVHGG
Sbjct: 61  RARYGSIFKSHILGCPTIVSMDPEVNRYVLMNESKGLVPGYPQSMLDILGKCNIAAVHGG 120

Query: 121 AHKLMRAALLAIVSPAMIKDRLLPKVDDFMRSHLRNWDRQIIDIQDKTKEMALRSSLKQI 180
           AHKLMR ALLAIVSP MIKDRLLPKVDDFMRSHLRNWD QIIDIQ+KTKEMALRSSLKQI
Sbjct: 121 AHKLMRGALLAIVSPTMIKDRLLPKVDDFMRSHLRNWDSQIIDIQEKTKEMALRSSLKQI 180

Query: 181 AGIESGPLSESFIPEFFKLVLGTLSLPIDLPGTNYRRGIQARKNIIKLLQQLINERRASK 240
           AGIESGPLSESFIPEFFKLVLGTLSLPIDLPGTNYRRGIQARKNIIKLLQQLINERR SK
Sbjct: 181 AGIESGPLSESFIPEFFKLVLGTLSLPIDLPGTNYRRGIQARKNIIKLLQQLINERRDSK 240

Query: 241 TEKDDMLGFFMSEENKYKLKDEEIIDQIITVLYSGYETVSTTSMMAVKFLHDHPKVLQQL 300
            +KDDMLGF MSEENKYKL DEEIIDQIITVLYSGYETVSTTSMMAVKFLHDHPKVLQQL
Sbjct: 241 IQKDDMLGFLMSEENKYKLNDEEIIDQIITVLYSGYETVSTTSMMAVKFLHDHPKVLQQL 300

Query: 301 REEHMAIRERKNPEDPIDWDDLKAMKFTRAVIFETSRLATIVNGVLRKTTKDMELNGFLI 360
           REEH+AIRE+KNPEDPIDWDDLKAMKFTRAVIFETSRLATIVNGVLRKTTKDMELNGFLI
Sbjct: 301 REEHLAIREKKNPEDPIDWDDLKAMKFTRAVIFETSRLATIVNGVLRKTTKDMELNGFLI 360

Query: 361 PKGWRIYVYTREINYDPFLYSEPFTFNPWRWLDKNLESHNYFFIFGGGSRLCPGKELGVA 420
           PKGWRIYVYTREINYDPFLYSEPFTFNPWRWLDKNLESHNYFFIFGGG+R CPGKELG+A
Sbjct: 361 PKGWRIYVYTREINYDPFLYSEPFTFNPWRWLDKNLESHNYFFIFGGGTRHCPGKELGIA 420

Query: 421 EISTFLHYFVTKYRWEEVGGDELLKFPRVEAPSGFHIRVSSY 463
           EISTFLHYFVTKYRWEEVGGD+LLKFPRVEAPSGFHIRVSSY
Sbjct: 421 EISTFLHYFVTKYRWEEVGGDKLLKFPRVEAPSGFHIRVSSY 462

[Close]

BLAST of Moc04g37060 vs. NCBI nr
Match: XP_022939540.1 (cytochrome P450 85A-like [Cucurbita moschata] >XP_023550225.1 cytochrome P450 85A-like [Cucurbita pepo subsp. pepo] >KAG7016098.1 Cytochrome P450 85A [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 894.8 bits (2311), Expect = 3.1e-256
Identity = 434/462 (93.94%), Postives = 452/462 (97.84%), Query Frame = 0


Query: 1   MAVAMAILALILLLCFCSALLRWNEVRFSKKGLPPGTMGWPVFGETTEFLKQGPNFMKTQ 60
           MA+AM ILAL LLLCFC+ALLRWNEVR+SKKGLPPGTMGWP+FGETTEFLKQGPNFMK+Q
Sbjct: 1   MALAMVILALFLLLCFCAALLRWNEVRYSKKGLPPGTMGWPIFGETTEFLKQGPNFMKSQ 60

Query: 61  RARYGSIFKSHILGCPTVVSMDPEVNRYVLMNESKGLVPGYPQSMLDILGKCNIAAVHGG 120
           RARYGSIFKSHILGCPT+V MDPEVNRYVLMNESKGLVPGYPQSMLDILGKCNIAAVHG 
Sbjct: 61  RARYGSIFKSHILGCPTIVCMDPEVNRYVLMNESKGLVPGYPQSMLDILGKCNIAAVHGS 120

Query: 121 AHKLMRAALLAIVSPAMIKDRLLPKVDDFMRSHLRNWDRQIIDIQDKTKEMALRSSLKQI 180
           AHKLMRAALL++VSP MIKDRLLPKVD+FMRSHLRNWDRQIIDIQDKTKEMAL SSLKQI
Sbjct: 121 AHKLMRAALLSVVSPTMIKDRLLPKVDEFMRSHLRNWDRQIIDIQDKTKEMALCSSLKQI 180

Query: 181 AGIESGPLSESFIPEFFKLVLGTLSLPIDLPGTNYRRGIQARKNIIKLLQQLINERRASK 240
           AGIESGPLSESFIPEFFKLVLGTLSLPID PGTNYR+GIQARK+IIKLLQ+LI+ERRASK
Sbjct: 181 AGIESGPLSESFIPEFFKLVLGTLSLPIDFPGTNYRQGIQARKSIIKLLQKLIDERRASK 240

Query: 241 TEKDDMLGFFMSEENKYKLKDEEIIDQIITVLYSGYETVSTTSMMAVKFLHDHPKVLQQL 300
           TEKDDMLGFFMSEENKYKLKDEEIIDQIITVLYSGYETVSTTSMMAVKFLHDHPKVLQQL
Sbjct: 241 TEKDDMLGFFMSEENKYKLKDEEIIDQIITVLYSGYETVSTTSMMAVKFLHDHPKVLQQL 300

Query: 301 REEHMAIRERKNPEDPIDWDDLKAMKFTRAVIFETSRLATIVNGVLRKTTKDMELNGFLI 360
           REEH+AIRE+KNPEDPIDWDDLKAMKFTRAVIFETSRLATIVNGVLRKTTKDMELNGFLI
Sbjct: 301 REEHLAIREKKNPEDPIDWDDLKAMKFTRAVIFETSRLATIVNGVLRKTTKDMELNGFLI 360

Query: 361 PKGWRIYVYTREINYDPFLYSEPFTFNPWRWLDKNLESHNYFFIFGGGSRLCPGKELGVA 420
           PKGWRIYVYTREINYD FLYSEP TFNPWRWLDKNLESHNYFFIFGGG+R CPGKELG+A
Sbjct: 361 PKGWRIYVYTREINYDSFLYSEPLTFNPWRWLDKNLESHNYFFIFGGGTRQCPGKELGIA 420

Query: 421 EISTFLHYFVTKYRWEEVGGDELLKFPRVEAPSGFHIRVSSY 463
           EISTFLHYFVTKYRWEEVGGD+LLKFPRVEAPSGFHIRVSSY
Sbjct: 421 EISTFLHYFVTKYRWEEVGGDKLLKFPRVEAPSGFHIRVSSY 462

[Close]

BLAST of Moc04g37060 vs. NCBI nr
Match: KAG6602538.1 (Cytochrome P450 85A, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 894.4 bits (2310), Expect = 4.1e-256
Identity = 430/462 (93.07%), Postives = 453/462 (98.05%), Query Frame = 0


Query: 1   MAVAMAILALILLLCFCSALLRWNEVRFSKKGLPPGTMGWPVFGETTEFLKQGPNFMKTQ 60
           MA+A+AILAL+LLLCFCSALLRWNEVR+ +KGLPPGTMGWP FGETTEFLKQGPNFMK+Q
Sbjct: 1   MALAIAILALVLLLCFCSALLRWNEVRYRRKGLPPGTMGWPFFGETTEFLKQGPNFMKSQ 60

Query: 61  RARYGSIFKSHILGCPTVVSMDPEVNRYVLMNESKGLVPGYPQSMLDILGKCNIAAVHGG 120
           RARYGSIFKSHILGCPT+VSMDPEVNRY+LMNESKGLVPGYPQSMLDILGKCNIAAVHGG
Sbjct: 61  RARYGSIFKSHILGCPTIVSMDPEVNRYILMNESKGLVPGYPQSMLDILGKCNIAAVHGG 120

Query: 121 AHKLMRAALLAIVSPAMIKDRLLPKVDDFMRSHLRNWDRQIIDIQDKTKEMALRSSLKQI 180
           AHK+MRAALL++VSP MI+DRLLPKVDDFMRSHLRNWD+QIIDIQ+KTKEMALRSS+KQI
Sbjct: 121 AHKVMRAALLSVVSPTMIRDRLLPKVDDFMRSHLRNWDQQIIDIQEKTKEMALRSSMKQI 180

Query: 181 AGIESGPLSESFIPEFFKLVLGTLSLPIDLPGTNYRRGIQARKNIIKLLQQLINERRASK 240
           AGIESGPLSESFIPEFFKLVLGTLSLPIDLPGTNYRRGIQARKNI+KLLQQLINERRAS+
Sbjct: 181 AGIESGPLSESFIPEFFKLVLGTLSLPIDLPGTNYRRGIQARKNIVKLLQQLINERRASE 240

Query: 241 TEKDDMLGFFMSEENKYKLKDEEIIDQIITVLYSGYETVSTTSMMAVKFLHDHPKVLQQL 300
           T+KDDMLGFFMSEENKYKL DEE+IDQIITVLYSGYETVSTTSMMAVKFLHDHPKVLQQL
Sbjct: 241 TQKDDMLGFFMSEENKYKLNDEEVIDQIITVLYSGYETVSTTSMMAVKFLHDHPKVLQQL 300

Query: 301 REEHMAIRERKNPEDPIDWDDLKAMKFTRAVIFETSRLATIVNGVLRKTTKDMELNGFLI 360
           REEH AIRE+K  EDPIDWDDLKAMKFTRAVIFETSRLATIVNGVLRKTTKDMELNGFLI
Sbjct: 301 REEHFAIREKKRAEDPIDWDDLKAMKFTRAVIFETSRLATIVNGVLRKTTKDMELNGFLI 360

Query: 361 PKGWRIYVYTREINYDPFLYSEPFTFNPWRWLDKNLESHNYFFIFGGGSRLCPGKELGVA 420
           PKGWRIYVYTREINYDPFLYSEP TFNPWRWLDKNLESHNYFFIFGGG+R CPGKELG+A
Sbjct: 361 PKGWRIYVYTREINYDPFLYSEPLTFNPWRWLDKNLESHNYFFIFGGGTRHCPGKELGIA 420

Query: 421 EISTFLHYFVTKYRWEEVGGDELLKFPRVEAPSGFHIRVSSY 463
           EISTFLHYFVTKYRWEEVGGD+LLKFPRVEAPSGFHIRVSSY
Sbjct: 421 EISTFLHYFVTKYRWEEVGGDKLLKFPRVEAPSGFHIRVSSY 462

[Close]

BLAST of Moc04g37060 vs. NCBI nr
Match: KAG7033215.1 (Cytochrome P450 85A [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 894.4 bits (2310), Expect = 4.1e-256
Identity = 430/462 (93.07%), Postives = 454/462 (98.27%), Query Frame = 0


Query: 1   MAVAMAILALILLLCFCSALLRWNEVRFSKKGLPPGTMGWPVFGETTEFLKQGPNFMKTQ 60
           MA+A+AILAL+LLLCFCSALLRWNEVR+ +KGLPPGTMGWP FGETTEFLKQGPNFMK+Q
Sbjct: 1   MALAIAILALVLLLCFCSALLRWNEVRYRRKGLPPGTMGWPFFGETTEFLKQGPNFMKSQ 60

Query: 61  RARYGSIFKSHILGCPTVVSMDPEVNRYVLMNESKGLVPGYPQSMLDILGKCNIAAVHGG 120
           RARYGSIFKSHILGCPT+VSMDPEVNRY+LMNESKGLVPGYPQSMLDILGKCNIAAVHGG
Sbjct: 61  RARYGSIFKSHILGCPTIVSMDPEVNRYILMNESKGLVPGYPQSMLDILGKCNIAAVHGG 120

Query: 121 AHKLMRAALLAIVSPAMIKDRLLPKVDDFMRSHLRNWDRQIIDIQDKTKEMALRSSLKQI 180
           AHK+MRAALL++VSP MI+DRLLPKVDDFMRSHLRNWD+QIIDIQ+KTKEMALRSS+KQI
Sbjct: 121 AHKVMRAALLSVVSPTMIRDRLLPKVDDFMRSHLRNWDQQIIDIQEKTKEMALRSSMKQI 180

Query: 181 AGIESGPLSESFIPEFFKLVLGTLSLPIDLPGTNYRRGIQARKNIIKLLQQLINERRASK 240
           AGIESGPLSESFIPEFFKLVLGTLSLPIDLPGTNYRRGIQARKNIIKLL+QLINERRAS+
Sbjct: 181 AGIESGPLSESFIPEFFKLVLGTLSLPIDLPGTNYRRGIQARKNIIKLLEQLINERRASE 240

Query: 241 TEKDDMLGFFMSEENKYKLKDEEIIDQIITVLYSGYETVSTTSMMAVKFLHDHPKVLQQL 300
           T+KDDMLGFFMSEENKYKL DEE+IDQIITVLYSGYETVSTTSMMAVKFLHDHPKVLQQL
Sbjct: 241 TQKDDMLGFFMSEENKYKLNDEEVIDQIITVLYSGYETVSTTSMMAVKFLHDHPKVLQQL 300

Query: 301 REEHMAIRERKNPEDPIDWDDLKAMKFTRAVIFETSRLATIVNGVLRKTTKDMELNGFLI 360
           REEH+AIRE+K  EDPIDWDDLKAMKFTRAVIFETSRLATIVNGVLRKTTKDMELNGFLI
Sbjct: 301 REEHLAIREKKRAEDPIDWDDLKAMKFTRAVIFETSRLATIVNGVLRKTTKDMELNGFLI 360

Query: 361 PKGWRIYVYTREINYDPFLYSEPFTFNPWRWLDKNLESHNYFFIFGGGSRLCPGKELGVA 420
           PKGWRIYVYTREINYDPFLYSEP TFNPWRWLDKNLESHNYFFIFGGG+R CPGKELG+A
Sbjct: 361 PKGWRIYVYTREINYDPFLYSEPLTFNPWRWLDKNLESHNYFFIFGGGTRHCPGKELGIA 420

Query: 421 EISTFLHYFVTKYRWEEVGGDELLKFPRVEAPSGFHIRVSSY 463
           EISTFLHYFVTKYRWEEVGGD+LLKFPRVEAPSGFHIRVSSY
Sbjct: 421 EISTFLHYFVTKYRWEEVGGDKLLKFPRVEAPSGFHIRVSSY 462

[Close]

BLAST of Moc04g37060 vs. ExPASy Swiss-Prot
Match: Q69F95 (Cytochrome P450 85A OS=Phaseolus vulgaris OX=3885 GN=BA13 PE=3 SV=2)

HSP 1 Score: 745.0 bits (1922), Expect = 5.2e-214
Identity = 354/464 (76.29%), Postives = 414/464 (89.22%), Query Frame = 0


Query: 1   MAVAMAIL-ALILLLCFCSALLRWNEVRFSKKGLPPGTMGWPVFGETTEFLKQGPNFMKT 60
           MA+ MAIL  L+LLLCFCSALL+WNEVRF +KGLPPG MGWPVFGETTEFLKQGPNFMK 
Sbjct: 1   MALFMAILGVLVLLLCFCSALLKWNEVRFRRKGLPPGAMGWPVFGETTEFLKQGPNFMKN 60

Query: 61  QRARYGSIFKSHILGCPTVVSMDPEVNRYVLMNESKGLVPGYPQSMLDILGKCNIAAVHG 120
           +RARYGS FKSHILGCPT+VSMDPE+NR++LMNE+KGLVPGYPQSMLDILG  NIAAVHG
Sbjct: 61  KRARYGSFFKSHILGCPTIVSMDPELNRFILMNEAKGLVPGYPQSMLDILGTRNIAAVHG 120

Query: 121 GAHKLMRAALLAIVSPAMIKDRLLPKVDDFMRSHLRNWDRQIIDIQDKTKEMALRSSLKQ 180
             HK MR ALL+I+SP +I+D+LLPK+D+FMR+HL +WD ++I+IQ+KTKEMA  SSLKQ
Sbjct: 121 STHKYMRGALLSIISPTLIRDQLLPKIDEFMRTHLMDWDNKVINIQEKTKEMAFLSSLKQ 180

Query: 181 IAGIESGPLSESFIPEFFKLVLGTLSLPIDLPGTNYRRGIQARKNIIKLLQQLINERRAS 240
           IAG+ES  +++ F+ EFFKLVLGTLSLPI+LP TNYR G+QARK+II +L QL+ ER+AS
Sbjct: 181 IAGMESSSIAQPFMTEFFKLVLGTLSLPINLPRTNYRGGLQARKSIISILSQLLEERKAS 240

Query: 241 KTEKDDMLGFFM-SEENKYKLKDEEIIDQIITVLYSGYETVSTTSMMAVKFLHDHPKVLQ 300
           +    DMLG  M  +EN+YKL DEEIID +IT++YSGYETVSTTSMMAVK+LHDHPKVL+
Sbjct: 241 QDVHVDMLGCLMKKDENRYKLSDEEIIDLVITIMYSGYETVSTTSMMAVKYLHDHPKVLE 300

Query: 301 QLREEHMAIRERKNPEDPIDWDDLKAMKFTRAVIFETSRLATIVNGVLRKTTKDMELNGF 360
           ++R+EH AIRERK PEDPID +DLK+M+FTRAVIFETSRLATIVNGVLRKTT DMELNG+
Sbjct: 301 EIRKEHFAIRERKKPEDPIDCNDLKSMRFTRAVIFETSRLATIVNGVLRKTTHDMELNGY 360

Query: 361 LIPKGWRIYVYTREINYDPFLYSEPFTFNPWRWLDKNLESHNYFFIFGGGSRLCPGKELG 420
           LIPKGWRIYVYTREINYDPFLY +P TFNPWRWL  +LES ++F IFGGG+R CPGKELG
Sbjct: 361 LIPKGWRIYVYTREINYDPFLYHDPLTFNPWRWLGNSLESQSHFLIFGGGTRQCPGKELG 420

Query: 421 VAEISTFLHYFVTKYRWEEVGGDELLKFPRVEAPSGFHIRVSSY 463
           +AEISTFLHYFVT+YRWEEVGGD+L+KFPRV AP+G HIRVSS+
Sbjct: 421 IAEISTFLHYFVTRYRWEEVGGDKLMKFPRVVAPNGLHIRVSSF 464

[Close]

BLAST of Moc04g37060 vs. ExPASy Swiss-Prot
Match: Q43147 (Cytochrome P450 85A1 OS=Solanum lycopersicum OX=4081 GN=CYP85A1 PE=2 SV=1)

HSP 1 Score: 721.5 bits (1861), Expect = 6.2e-207
Identity = 339/464 (73.06%), Postives = 409/464 (88.15%), Query Frame = 0


Query: 1   MAVAMAILALILLLC-FCSALLRWNEVRFSKKGLPPGTMGWPVFGETTEFLKQGPNFMKT 60
           MA  +  L+    LC FC+ALLRWN+V++++K LPPGTMGWP+FGETTEFLK GP+FMK 
Sbjct: 1   MAFFLIFLSSFFGLCIFCTALLRWNQVKYNQKNLPPGTMGWPLFGETTEFLKLGPSFMKN 60

Query: 61  QRARYGSIFKSHILGCPTVVSMDPEVNRYVLMNESKGLVPGYPQSMLDILGKCNIAAVHG 120
           QRARYGS FKSHILGCPT+VSMD E+NRY+L+NE+KGLVPGYPQSM+DILGKCNIAAV+G
Sbjct: 61  QRARYGSFFKSHILGCPTIVSMDSELNRYILVNEAKGLVPGYPQSMIDILGKCNIAAVNG 120

Query: 121 GAHKLMRAALLAIVSPAMIKDRLLPKVDDFMRSHLRNWDRQIIDIQDKTKEMALRSSLKQ 180
            AHK MR ALL+++SP MI+D+LLPK+D+FMRSHL NWD ++IDIQ+KT +MA  SSLKQ
Sbjct: 121 SAHKYMRGALLSLISPTMIRDQLLPKIDEFMRSHLTNWDNKVIDIQEKTNKMAFLSSLKQ 180

Query: 181 IAGIESGPLSESFIPEFFKLVLGTLSLPIDLPGTNYRRGIQARKNIIKLLQQLINERRAS 240
           IAGIES  L++ F+ EFF LVLGTLSLPI+LP TNY RG QARK I+ LL+ LI ERRAS
Sbjct: 181 IAGIESTSLAQEFMSEFFNLVLGTLSLPINLPNTNYHRGFQARKIIVNLLRTLIEERRAS 240

Query: 241 KTEKDDMLGFFMSEE-NKYKLKDEEIIDQIITVLYSGYETVSTTSMMAVKFLHDHPKVLQ 300
           K  + DMLG+ M+EE  ++KL D+E+ID IIT+LYSGYETVSTTSMMAVK+LHDHPKVL+
Sbjct: 241 KEIQHDMLGYLMNEEATRFKLTDDEMIDLIITILYSGYETVSTTSMMAVKYLHDHPKVLE 300

Query: 301 QLREEHMAIRERKNPEDPIDWDDLKAMKFTRAVIFETSRLATIVNGVLRKTTKDMELNGF 360
           +LR+EHMAIRE+K PEDPID++D ++M+FTRAVI ETSRLATIVNGVLRKTT+DME+NG+
Sbjct: 301 ELRKEHMAIREKKKPEDPIDYNDYRSMRFTRAVILETSRLATIVNGVLRKTTQDMEINGY 360

Query: 361 LIPKGWRIYVYTREINYDPFLYSEPFTFNPWRWLDKNLESHNYFFIFGGGSRLCPGKELG 420
           +IPKGWRIYVYTRE+NYDP LY +P++FNPWRW+DK+LE  N F +FGGG+R CPGKELG
Sbjct: 361 IIPKGWRIYVYTRELNYDPRLYPDPYSFNPWRWMDKSLEHQNSFLVFGGGTRQCPGKELG 420

Query: 421 VAEISTFLHYFVTKYRWEEVGGDELLKFPRVEAPSGFHIRVSSY 463
           VAEISTFLHYFVTKYRWEE+GGD+L+KFPRVEAP+G  IRVS++
Sbjct: 421 VAEISTFLHYFVTKYRWEEIGGDKLMKFPRVEAPNGLRIRVSAH 464

[Close]

BLAST of Moc04g37060 vs. ExPASy Swiss-Prot
Match: Q940V4 (Cytochrome P450 85A2 OS=Arabidopsis thaliana OX=3702 GN=CYP85A2 PE=2 SV=1)

HSP 1 Score: 686.0 bits (1769), Expect = 2.9e-196
Identity = 322/465 (69.25%), Postives = 392/465 (84.30%), Query Frame = 0


Query: 1   MAVAMAILA-LILLLCFCSALLRWNEVRFSKKGLPPGTMGWPVFGETTEFLKQGPNFMKT 60
           M + M IL  L++++C C+ALLRWN++R+SKKGLPPGTMGWP+FGETTEFLKQGP+FMK 
Sbjct: 1   MGIMMMILGLLVIIVCLCTALLRWNQMRYSKKGLPPGTMGWPIFGETTEFLKQGPDFMKN 60

Query: 61  QRARYGSIFKSHILGCPTVVSMDPEVNRYVLMNESKGLVPGYPQSMLDILGKCNIAAVHG 120
           QR RYGS FKSHILGCPT+VSMD E+NRY+LMNESKGLV GYPQSMLDILG CNIAAVHG
Sbjct: 61  QRLRYGSFFKSHILGCPTIVSMDAELNRYILMNESKGLVAGYPQSMLDILGTCNIAAVHG 120

Query: 121 GAHKLMRAALLAIVSPAMIKDRLLPKVDDFMRSHLRNW-DRQIIDIQDKTKEMALRSSLK 180
            +H+LMR +LL+++SP M+KD LLPK+DDFMR++L  W D + +DIQ+KTK MA  SSL 
Sbjct: 121 PSHRLMRGSLLSLISPTMMKDHLLPKIDDFMRNYLCGWDDLETVDIQEKTKHMAFLSSLL 180

Query: 181 QIAGIESGPLSESFIPEFFKLVLGTLSLPIDLPGTNYRRGIQARKNIIKLLQQLINERRA 240
           QIA     P  E +  EFFKLV+GTLS+PID+PGTNYR G+QAR NI +LL +L+ ER+ 
Sbjct: 181 QIAETLKKPEVEEYRTEFFKLVVGTLSVPIDIPGTNYRSGVQARNNIDRLLTELMQERKE 240

Query: 241 SKTEKDDMLGFFM-SEENKYKLKDEEIIDQIITVLYSGYETVSTTSMMAVKFLHDHPKVL 300
           S     DMLG+ M  E+N+Y L D+EI DQ++T+LYSGYETVSTTSMMA+K+LHDHPK L
Sbjct: 241 SGETFTDMLGYLMKKEDNRYLLTDKEIRDQVVTILYSGYETVSTTSMMALKYLHDHPKAL 300

Query: 301 QQLREEHMAIRERKNPEDPIDWDDLKAMKFTRAVIFETSRLATIVNGVLRKTTKDMELNG 360
           ++LR EH+AIRERK P++P+  DD+K+MKFTRAVIFETSRLATIVNGVLRKTT D+ELNG
Sbjct: 301 EELRREHLAIRERKRPDEPLTLDDIKSMKFTRAVIFETSRLATIVNGVLRKTTHDLELNG 360

Query: 361 FLIPKGWRIYVYTREINYDPFLYSEPFTFNPWRWLDKNLESHNYFFIFGGGSRLCPGKEL 420
           +LIPKGWRIYVYTREINYD  LY +P  FNPWRW++K+LES +YF +FGGG RLCPGKEL
Sbjct: 361 YLIPKGWRIYVYTREINYDTSLYEDPMIFNPWRWMEKSLESKSYFLLFGGGVRLCPGKEL 420

Query: 421 GVAEISTFLHYFVTKYRWEEVGGDELLKFPRVEAPSGFHIRVSSY 463
           G++E+S+FLHYFVTKYRWEE G D+L+ FPRV AP G+H++ S Y
Sbjct: 421 GISEVSSFLHYFVTKYRWEENGEDKLMVFPRVSAPKGYHLKCSPY 465

[Close]

BLAST of Moc04g37060 vs. ExPASy Swiss-Prot
Match: Q9FMA5 (Cytochrome P450 85A1 OS=Arabidopsis thaliana OX=3702 GN=CYP85A1 PE=2 SV=1)

HSP 1 Score: 681.0 bits (1756), Expect = 9.3e-195
Identity = 321/465 (69.03%), Postives = 389/465 (83.66%), Query Frame = 0


Query: 1   MAVAMAILALILLL-CFCSALLRWNEVRFSKKGLPPGTMGWPVFGETTEFLKQGPNFMKT 60
           M   M ++ L+L++   CSALLRWN++R++K GLPPGTMGWP+FGETTEFLKQGPNFM+ 
Sbjct: 1   MGAMMVMMGLLLIIVSLCSALLRWNQMRYTKNGLPPGTMGWPIFGETTEFLKQGPNFMRN 60

Query: 61  QRARYGSIFKSHILGCPTVVSMDPEVNRYVLMNESKGLVPGYPQSMLDILGKCNIAAVHG 120
           QR RYGS FKSH+LGCPT++SMD EVNRY+L NESKGLVPGYPQSMLDILG CN+AAVHG
Sbjct: 61  QRLRYGSFFKSHLLGCPTLISMDSEVNRYILKNESKGLVPGYPQSMLDILGTCNMAAVHG 120

Query: 121 GAHKLMRAALLAIVSPAMIKDRLLPKVDDFMRSHLRNWDR-QIIDIQDKTKEMALRSSLK 180
            +H+LMR +LL+++S  M++D +LPKVD FMRS+L  W+  ++IDIQDKTK MA  SSL 
Sbjct: 121 SSHRLMRGSLLSLISSTMMRDHILPKVDHFMRSYLDQWNELEVIDIQDKTKHMAFLSSLT 180

Query: 181 QIAGIESGPLSESFIPEFFKLVLGTLSLPIDLPGTNYRRGIQARKNIIKLLQQLINERRA 240
           QIAG    P  E F   FFKLV+GTLS+PIDLPGTNYR GIQAR NI +LL++L+ ERR 
Sbjct: 181 QIAGNLRKPFVEEFKTAFFKLVVGTLSVPIDLPGTNYRCGIQARNNIDRLLRELMQERRD 240

Query: 241 SKTEKDDMLGFFMSEE-NKYKLKDEEIIDQIITVLYSGYETVSTTSMMAVKFLHDHPKVL 300
           S     DMLG+ M +E N+Y L DEEI DQ++T+LYSGYETVSTTSMMA+K+LHDHPK L
Sbjct: 241 SGETFTDMLGYLMKKEGNRYPLTDEEIRDQVVTILYSGYETVSTTSMMALKYLHDHPKAL 300

Query: 301 QQLREEHMAIRERKNPEDPIDWDDLKAMKFTRAVIFETSRLATIVNGVLRKTTKDMELNG 360
           Q+LR EH+A RERK  ++P+  +D+K+MKFTRAVI+ETSRLATIVNGVLRKTT+D+E+NG
Sbjct: 301 QELRAEHLAFRERKRQDEPLGLEDVKSMKFTRAVIYETSRLATIVNGVLRKTTRDLEING 360

Query: 361 FLIPKGWRIYVYTREINYDPFLYSEPFTFNPWRWLDKNLESHNYFFIFGGGSRLCPGKEL 420
           +LIPKGWRIYVYTREINYD  LY +P  FNPWRW+ K+LES N  F+FGGG+RLCPGKEL
Sbjct: 361 YLIPKGWRIYVYTREINYDANLYEDPLIFNPWRWMKKSLESQNSCFVFGGGTRLCPGKEL 420

Query: 421 GVAEISTFLHYFVTKYRWEEVGGDELLKFPRVEAPSGFHIRVSSY 463
           G+ EIS+FLHYFVT+YRWEE+GGDEL+ FPRV AP GFH+R+S Y
Sbjct: 421 GIVEISSFLHYFVTRYRWEEIGGDELMVFPRVFAPKGFHLRISPY 465

[Close]

BLAST of Moc04g37060 vs. ExPASy Swiss-Prot
Match: Q50LE0 (Cytochrome P450 85A3 OS=Solanum lycopersicum OX=4081 GN=CYP85A3 PE=2 SV=1)

HSP 1 Score: 658.7 bits (1698), Expect = 4.9e-188
Identity = 312/467 (66.81%), Postives = 382/467 (81.80%), Query Frame = 0


Query: 1   MAVAMAILALILLLCFCSA-LLRWNEVRFSKKGLPPGTMGWPVFGETTEFLKQGPNFMKT 60
           MA+ + I  +    C  S  L RW ++ ++KK LPPGTMGWP+FGET EFL QGPNFMK 
Sbjct: 1   MAIFLIIFVVFFGFCILSTPLFRWIDIVYNKKNLPPGTMGWPIFGETREFLNQGPNFMKN 60

Query: 61  QRARYGSIFKSHILGCPTVVSMDPEVNRYVLMNESKGLVPGYPQSMLDILGKCNIAAVHG 120
           QRARYG+ FKSHILGCPTVVSMD  +N Y+L NE+KGL+PGYPQSMLDILGKCNIAAVHG
Sbjct: 61  QRARYGNFFKSHILGCPTVVSMDAGLNVYILNNEAKGLIPGYPQSMLDILGKCNIAAVHG 120

Query: 121 GAHKLMRAALLAIVSPAMIKDRLLPKVDDFMRSHLRNWDR-QIIDIQDKTKEMALRSSLK 180
             HK +R ALL++++P MIKD +LPK+D FMRSHL  WD   +IDIQ  TKEMA  SSL 
Sbjct: 121 ATHKYIRGALLSLINPTMIKDHILPKIDKFMRSHLSGWDNCNVIDIQQMTKEMAFFSSLD 180

Query: 181 QIAGI-ESGPLSESFIPEFFKLVLGTLSLPIDLPGTNYRRGIQARKNIIKLLQQLINERR 240
           QI G   S  +++ F   F  + LGT+SLPI+ P TNY RG+Q RK I+KLL+++I +RR
Sbjct: 181 QIGGFATSSSIAQEFRAGFLNIALGTISLPINFPTTNYYRGLQGRKTIVKLLRKIIEDRR 240

Query: 241 ASKTEKDDMLGFFMSEE--NKYKLKDEEIIDQIITVLYSGYETVSTTSMMAVKFLHDHPK 300
            SK  + DMLG  M+EE  N+Y L DEE+IDQIIT++YSG+ETVSTTSMMAVK+LHDHPK
Sbjct: 241 GSKKIQQDMLGLMMNEEAKNRYTLSDEELIDQIITIMYSGFETVSTTSMMAVKYLHDHPK 300

Query: 301 VLQQLREEHMAIRERKNPEDPIDWDDLKAMKFTRAVIFETSRLATIVNGVLRKTTKDMEL 360
            L+++R+EH AIRE+K+ EDPID++D KAM+FTRAVI+ET RLATIVNGVLRKTT+DMEL
Sbjct: 301 ALEEIRKEHFAIREKKSLEDPIDYNDFKAMRFTRAVIYETLRLATIVNGVLRKTTQDMEL 360

Query: 361 NGFLIPKGWRIYVYTREINYDPFLYSEPFTFNPWRWLDKNLESHNYFFIFGGGSRLCPGK 420
           NG++IPKGWRIYVYTRE+NYDP +Y +P+TFNPWRWL+ NL+  + F +FGGG+RLCPGK
Sbjct: 361 NGYMIPKGWRIYVYTRELNYDPLIYPDPYTFNPWRWLENNLDHQSSFLMFGGGTRLCPGK 420

Query: 421 ELGVAEISTFLHYFVTKYRWEEVGGDELLKFPRVEAPSGFHIRVSSY 463
           ELGVAEISTFLHYFVT+YRWEEVGG++L+KFPRVEA +G  I+VS+Y
Sbjct: 421 ELGVAEISTFLHYFVTRYRWEEVGGNKLMKFPRVEALNGLWIKVSAY 467

[Close]

BLAST of Moc04g37060 vs. ExPASy TrEMBL
Match: A0A6J1C1V2 (cytochrome P450 85A-like OS=Momordica charantia OX=3673 GN=LOC111006661 PE=3 SV=1)

HSP 1 Score: 937.2 bits (2421), Expect = 2.6e-269
Identity = 462/462 (100.00%), Postives = 462/462 (100.00%), Query Frame = 0


Query: 1   MAVAMAILALILLLCFCSALLRWNEVRFSKKGLPPGTMGWPVFGETTEFLKQGPNFMKTQ 60
           MAVAMAILALILLLCFCSALLRWNEVRFSKKGLPPGTMGWPVFGETTEFLKQGPNFMKTQ
Sbjct: 1   MAVAMAILALILLLCFCSALLRWNEVRFSKKGLPPGTMGWPVFGETTEFLKQGPNFMKTQ 60

Query: 61  RARYGSIFKSHILGCPTVVSMDPEVNRYVLMNESKGLVPGYPQSMLDILGKCNIAAVHGG 120
           RARYGSIFKSHILGCPTVVSMDPEVNRYVLMNESKGLVPGYPQSMLDILGKCNIAAVHGG
Sbjct: 61  RARYGSIFKSHILGCPTVVSMDPEVNRYVLMNESKGLVPGYPQSMLDILGKCNIAAVHGG 120

Query: 121 AHKLMRAALLAIVSPAMIKDRLLPKVDDFMRSHLRNWDRQIIDIQDKTKEMALRSSLKQI 180
           AHKLMRAALLAIVSPAMIKDRLLPKVDDFMRSHLRNWDRQIIDIQDKTKEMALRSSLKQI
Sbjct: 121 AHKLMRAALLAIVSPAMIKDRLLPKVDDFMRSHLRNWDRQIIDIQDKTKEMALRSSLKQI 180

Query: 181 AGIESGPLSESFIPEFFKLVLGTLSLPIDLPGTNYRRGIQARKNIIKLLQQLINERRASK 240
           AGIESGPLSESFIPEFFKLVLGTLSLPIDLPGTNYRRGIQARKNIIKLLQQLINERRASK
Sbjct: 181 AGIESGPLSESFIPEFFKLVLGTLSLPIDLPGTNYRRGIQARKNIIKLLQQLINERRASK 240

Query: 241 TEKDDMLGFFMSEENKYKLKDEEIIDQIITVLYSGYETVSTTSMMAVKFLHDHPKVLQQL 300
           TEKDDMLGFFMSEENKYKLKDEEIIDQIITVLYSGYETVSTTSMMAVKFLHDHPKVLQQL
Sbjct: 241 TEKDDMLGFFMSEENKYKLKDEEIIDQIITVLYSGYETVSTTSMMAVKFLHDHPKVLQQL 300

Query: 301 REEHMAIRERKNPEDPIDWDDLKAMKFTRAVIFETSRLATIVNGVLRKTTKDMELNGFLI 360
           REEHMAIRERKNPEDPIDWDDLKAMKFTRAVIFETSRLATIVNGVLRKTTKDMELNGFLI
Sbjct: 301 REEHMAIRERKNPEDPIDWDDLKAMKFTRAVIFETSRLATIVNGVLRKTTKDMELNGFLI 360

Query: 361 PKGWRIYVYTREINYDPFLYSEPFTFNPWRWLDKNLESHNYFFIFGGGSRLCPGKELGVA 420
           PKGWRIYVYTREINYDPFLYSEPFTFNPWRWLDKNLESHNYFFIFGGGSRLCPGKELGVA
Sbjct: 361 PKGWRIYVYTREINYDPFLYSEPFTFNPWRWLDKNLESHNYFFIFGGGSRLCPGKELGVA 420

Query: 421 EISTFLHYFVTKYRWEEVGGDELLKFPRVEAPSGFHIRVSSY 463
           EISTFLHYFVTKYRWEEVGGDELLKFPRVEAPSGFHIRVSSY
Sbjct: 421 EISTFLHYFVTKYRWEEVGGDELLKFPRVEAPSGFHIRVSSY 462

[Close]

BLAST of Moc04g37060 vs. ExPASy TrEMBL
Match: A0A6J1FN01 (cytochrome P450 85A-like OS=Cucurbita moschata OX=3662 GN=LOC111445417 PE=3 SV=1)

HSP 1 Score: 894.8 bits (2311), Expect = 1.5e-256
Identity = 434/462 (93.94%), Postives = 452/462 (97.84%), Query Frame = 0


Query: 1   MAVAMAILALILLLCFCSALLRWNEVRFSKKGLPPGTMGWPVFGETTEFLKQGPNFMKTQ 60
           MA+AM ILAL LLLCFC+ALLRWNEVR+SKKGLPPGTMGWP+FGETTEFLKQGPNFMK+Q
Sbjct: 1   MALAMVILALFLLLCFCAALLRWNEVRYSKKGLPPGTMGWPIFGETTEFLKQGPNFMKSQ 60

Query: 61  RARYGSIFKSHILGCPTVVSMDPEVNRYVLMNESKGLVPGYPQSMLDILGKCNIAAVHGG 120
           RARYGSIFKSHILGCPT+V MDPEVNRYVLMNESKGLVPGYPQSMLDILGKCNIAAVHG 
Sbjct: 61  RARYGSIFKSHILGCPTIVCMDPEVNRYVLMNESKGLVPGYPQSMLDILGKCNIAAVHGS 120

Query: 121 AHKLMRAALLAIVSPAMIKDRLLPKVDDFMRSHLRNWDRQIIDIQDKTKEMALRSSLKQI 180
           AHKLMRAALL++VSP MIKDRLLPKVD+FMRSHLRNWDRQIIDIQDKTKEMAL SSLKQI
Sbjct: 121 AHKLMRAALLSVVSPTMIKDRLLPKVDEFMRSHLRNWDRQIIDIQDKTKEMALCSSLKQI 180

Query: 181 AGIESGPLSESFIPEFFKLVLGTLSLPIDLPGTNYRRGIQARKNIIKLLQQLINERRASK 240
           AGIESGPLSESFIPEFFKLVLGTLSLPID PGTNYR+GIQARK+IIKLLQ+LI+ERRASK
Sbjct: 181 AGIESGPLSESFIPEFFKLVLGTLSLPIDFPGTNYRQGIQARKSIIKLLQKLIDERRASK 240

Query: 241 TEKDDMLGFFMSEENKYKLKDEEIIDQIITVLYSGYETVSTTSMMAVKFLHDHPKVLQQL 300
           TEKDDMLGFFMSEENKYKLKDEEIIDQIITVLYSGYETVSTTSMMAVKFLHDHPKVLQQL
Sbjct: 241 TEKDDMLGFFMSEENKYKLKDEEIIDQIITVLYSGYETVSTTSMMAVKFLHDHPKVLQQL 300

Query: 301 REEHMAIRERKNPEDPIDWDDLKAMKFTRAVIFETSRLATIVNGVLRKTTKDMELNGFLI 360
           REEH+AIRE+KNPEDPIDWDDLKAMKFTRAVIFETSRLATIVNGVLRKTTKDMELNGFLI
Sbjct: 301 REEHLAIREKKNPEDPIDWDDLKAMKFTRAVIFETSRLATIVNGVLRKTTKDMELNGFLI 360

Query: 361 PKGWRIYVYTREINYDPFLYSEPFTFNPWRWLDKNLESHNYFFIFGGGSRLCPGKELGVA 420
           PKGWRIYVYTREINYD FLYSEP TFNPWRWLDKNLESHNYFFIFGGG+R CPGKELG+A
Sbjct: 361 PKGWRIYVYTREINYDSFLYSEPLTFNPWRWLDKNLESHNYFFIFGGGTRQCPGKELGIA 420

Query: 421 EISTFLHYFVTKYRWEEVGGDELLKFPRVEAPSGFHIRVSSY 463
           EISTFLHYFVTKYRWEEVGGD+LLKFPRVEAPSGFHIRVSSY
Sbjct: 421 EISTFLHYFVTKYRWEEVGGDKLLKFPRVEAPSGFHIRVSSY 462

[Close]

BLAST of Moc04g37060 vs. ExPASy TrEMBL
Match: A0A6J1JUV6 (cytochrome P450 85A-like OS=Cucurbita maxima OX=3661 GN=LOC111489110 PE=3 SV=1)

HSP 1 Score: 893.3 bits (2307), Expect = 4.4e-256
Identity = 434/462 (93.94%), Postives = 451/462 (97.62%), Query Frame = 0


Query: 1   MAVAMAILALILLLCFCSALLRWNEVRFSKKGLPPGTMGWPVFGETTEFLKQGPNFMKTQ 60
           MA+AM ILAL LLLCFC+ALLRWNEVR+SKKGLPPGTMGWP+FGETTEFLKQGPNFMK+Q
Sbjct: 1   MALAMVILALFLLLCFCAALLRWNEVRYSKKGLPPGTMGWPIFGETTEFLKQGPNFMKSQ 60

Query: 61  RARYGSIFKSHILGCPTVVSMDPEVNRYVLMNESKGLVPGYPQSMLDILGKCNIAAVHGG 120
           RARYGSIFKSHILGCPT+V MDPEVNRYVLMNESKGLVPGYPQSMLDILGKCNIAAVHG 
Sbjct: 61  RARYGSIFKSHILGCPTIVCMDPEVNRYVLMNESKGLVPGYPQSMLDILGKCNIAAVHGS 120

Query: 121 AHKLMRAALLAIVSPAMIKDRLLPKVDDFMRSHLRNWDRQIIDIQDKTKEMALRSSLKQI 180
           AHKLMRAALL++VSP MIKDRLLPKVD+FMRSHLRNWDRQIIDIQDKTKEMAL SSLKQI
Sbjct: 121 AHKLMRAALLSVVSPTMIKDRLLPKVDEFMRSHLRNWDRQIIDIQDKTKEMALCSSLKQI 180

Query: 181 AGIESGPLSESFIPEFFKLVLGTLSLPIDLPGTNYRRGIQARKNIIKLLQQLINERRASK 240
           AGIESGPLSESFIPEFFKLVLGTLSLPID PGTNYR+GIQARK IIKLLQ+LI+ERRASK
Sbjct: 181 AGIESGPLSESFIPEFFKLVLGTLSLPIDFPGTNYRQGIQARKIIIKLLQKLIDERRASK 240

Query: 241 TEKDDMLGFFMSEENKYKLKDEEIIDQIITVLYSGYETVSTTSMMAVKFLHDHPKVLQQL 300
           TEKDDMLGFFMSEENKYKLKDEEIIDQIITVLYSGYETVSTTSMMAVKFLHDHPKVLQQL
Sbjct: 241 TEKDDMLGFFMSEENKYKLKDEEIIDQIITVLYSGYETVSTTSMMAVKFLHDHPKVLQQL 300

Query: 301 REEHMAIRERKNPEDPIDWDDLKAMKFTRAVIFETSRLATIVNGVLRKTTKDMELNGFLI 360
           REEH+AIRE+KNPEDPIDWDDLKAMKFTRAVIFETSRLATIVNGVLRKTTKDMELNGFLI
Sbjct: 301 REEHLAIREKKNPEDPIDWDDLKAMKFTRAVIFETSRLATIVNGVLRKTTKDMELNGFLI 360

Query: 361 PKGWRIYVYTREINYDPFLYSEPFTFNPWRWLDKNLESHNYFFIFGGGSRLCPGKELGVA 420
           PKGWRIYVYTREINYD FLYSEP TFNPWRWLDKNLESHNYFFIFGGG+R CPGKELG+A
Sbjct: 361 PKGWRIYVYTREINYDSFLYSEPLTFNPWRWLDKNLESHNYFFIFGGGTRQCPGKELGIA 420

Query: 421 EISTFLHYFVTKYRWEEVGGDELLKFPRVEAPSGFHIRVSSY 463
           EISTFLHYFVTKYRWEEVGGD+LLKFPRVEAPSGFHIRVSSY
Sbjct: 421 EISTFLHYFVTKYRWEEVGGDKLLKFPRVEAPSGFHIRVSSY 462

[Close]

BLAST of Moc04g37060 vs. ExPASy TrEMBL
Match: A0A6J1GNX1 (cytochrome P450 85A-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111455792 PE=3 SV=1)

HSP 1 Score: 888.6 bits (2295), Expect = 1.1e-254
Identity = 428/462 (92.64%), Postives = 451/462 (97.62%), Query Frame = 0


Query: 1   MAVAMAILALILLLCFCSALLRWNEVRFSKKGLPPGTMGWPVFGETTEFLKQGPNFMKTQ 60
           MA+A+AILAL+LLLCFCSALLRWNEVR+ +KGLPPGTMGWP FGETTEFLKQGPNFMK+Q
Sbjct: 1   MALAIAILALVLLLCFCSALLRWNEVRYRRKGLPPGTMGWPFFGETTEFLKQGPNFMKSQ 60

Query: 61  RARYGSIFKSHILGCPTVVSMDPEVNRYVLMNESKGLVPGYPQSMLDILGKCNIAAVHGG 120
           RARY  +FKSHILGCPT+VSMDPEVNRYVLMNESKGLVPGYPQSMLDILGKCNIAAVHGG
Sbjct: 61  RARYPFLFKSHILGCPTIVSMDPEVNRYVLMNESKGLVPGYPQSMLDILGKCNIAAVHGG 120

Query: 121 AHKLMRAALLAIVSPAMIKDRLLPKVDDFMRSHLRNWDRQIIDIQDKTKEMALRSSLKQI 180
           AHK+MRAALL++VSP MI+DRLLPKVDDFMRSHLRNWD+QIIDIQ+KTKEMALRSS+KQI
Sbjct: 121 AHKVMRAALLSVVSPTMIRDRLLPKVDDFMRSHLRNWDQQIIDIQEKTKEMALRSSMKQI 180

Query: 181 AGIESGPLSESFIPEFFKLVLGTLSLPIDLPGTNYRRGIQARKNIIKLLQQLINERRASK 240
           AGIESGPLSESFIPEFFKLVLGTLSLPIDLPGTNYRRGIQARKNI+KLLQQLINERRAS+
Sbjct: 181 AGIESGPLSESFIPEFFKLVLGTLSLPIDLPGTNYRRGIQARKNIVKLLQQLINERRASE 240

Query: 241 TEKDDMLGFFMSEENKYKLKDEEIIDQIITVLYSGYETVSTTSMMAVKFLHDHPKVLQQL 300
           T+KDDMLGFFMSEENKYKL DEE+IDQIITVLYSGYETVSTTSMMAVKFLHDHPKVLQQL
Sbjct: 241 TQKDDMLGFFMSEENKYKLNDEEVIDQIITVLYSGYETVSTTSMMAVKFLHDHPKVLQQL 300

Query: 301 REEHMAIRERKNPEDPIDWDDLKAMKFTRAVIFETSRLATIVNGVLRKTTKDMELNGFLI 360
           REEH AIRE+K  EDPIDWDDLKAMKFTRAVIFETSRLATIVNGVLRKTTKDMELNGFLI
Sbjct: 301 REEHFAIREKKRAEDPIDWDDLKAMKFTRAVIFETSRLATIVNGVLRKTTKDMELNGFLI 360

Query: 361 PKGWRIYVYTREINYDPFLYSEPFTFNPWRWLDKNLESHNYFFIFGGGSRLCPGKELGVA 420
           PKGWRIYVYTREINYDPFLYSEP TFNPWRWLDKNLESHNYFFIFGGG+R CPGKELG+A
Sbjct: 361 PKGWRIYVYTREINYDPFLYSEPLTFNPWRWLDKNLESHNYFFIFGGGTRHCPGKELGIA 420

Query: 421 EISTFLHYFVTKYRWEEVGGDELLKFPRVEAPSGFHIRVSSY 463
           EISTFLHYFVTKYRWEEVGGD+LLKFPRVEAPSGFHIRVSSY
Sbjct: 421 EISTFLHYFVTKYRWEEVGGDKLLKFPRVEAPSGFHIRVSSY 462

[Close]

BLAST of Moc04g37060 vs. ExPASy TrEMBL
Match: A0A6J1JRL1 (cytochrome P450 85A-like OS=Cucurbita maxima OX=3661 GN=LOC111487713 PE=3 SV=1)

HSP 1 Score: 888.3 bits (2294), Expect = 1.4e-254
Identity = 429/462 (92.86%), Postives = 452/462 (97.84%), Query Frame = 0


Query: 1   MAVAMAILALILLLCFCSALLRWNEVRFSKKGLPPGTMGWPVFGETTEFLKQGPNFMKTQ 60
           MA+A+AILAL LLLCFCSALLRWNEVR+ +KGLP GTMGWP FGETTEFLKQGPNFMK+Q
Sbjct: 1   MALAIAILALFLLLCFCSALLRWNEVRYRRKGLPHGTMGWPFFGETTEFLKQGPNFMKSQ 60

Query: 61  RARYGSIFKSHILGCPTVVSMDPEVNRYVLMNESKGLVPGYPQSMLDILGKCNIAAVHGG 120
           RARYGSIFKSHILGCPT+VSMDPEVNRYVLMNESKGLVPGYPQSMLDILGKCNIAAVHGG
Sbjct: 61  RARYGSIFKSHILGCPTIVSMDPEVNRYVLMNESKGLVPGYPQSMLDILGKCNIAAVHGG 120

Query: 121 AHKLMRAALLAIVSPAMIKDRLLPKVDDFMRSHLRNWDRQIIDIQDKTKEMALRSSLKQI 180
           AHK+MRAALL++VSP MI+DRLLPKVDDFMRSHLRNWD+QIIDIQ+KTKEMALRSS+KQI
Sbjct: 121 AHKVMRAALLSVVSPTMIRDRLLPKVDDFMRSHLRNWDQQIIDIQEKTKEMALRSSMKQI 180

Query: 181 AGIESGPLSESFIPEFFKLVLGTLSLPIDLPGTNYRRGIQARKNIIKLLQQLINERRASK 240
           AGIESGPLSESFIPEFFKLVLGTLSLPIDLPGTNYRRGIQARK+IIKLLQQLINERRA++
Sbjct: 181 AGIESGPLSESFIPEFFKLVLGTLSLPIDLPGTNYRRGIQARKSIIKLLQQLINERRATE 240

Query: 241 TEKDDMLGFFMSEENKYKLKDEEIIDQIITVLYSGYETVSTTSMMAVKFLHDHPKVLQQL 300
           T+KDDMLGFFMSEENKYKL DEE+IDQIITVLYSGYETVSTTSMMAVKFLHDHPKVLQQL
Sbjct: 241 TQKDDMLGFFMSEENKYKLNDEEVIDQIITVLYSGYETVSTTSMMAVKFLHDHPKVLQQL 300

Query: 301 REEHMAIRERKNPEDPIDWDDLKAMKFTRAVIFETSRLATIVNGVLRKTTKDMELNGFLI 360
           REEH+AIRE+K  EDPIDWDDLKAMKFTRAVIFETSRLATIVNGVLRKTTKDMELNGFLI
Sbjct: 301 REEHLAIREKKRAEDPIDWDDLKAMKFTRAVIFETSRLATIVNGVLRKTTKDMELNGFLI 360

Query: 361 PKGWRIYVYTREINYDPFLYSEPFTFNPWRWLDKNLESHNYFFIFGGGSRLCPGKELGVA 420
           PKGWRIYVYTREINYDPFLYSEP TFNPWRWLDKNLESHNYFFIFGGG+R CPGKELG+A
Sbjct: 361 PKGWRIYVYTREINYDPFLYSEPLTFNPWRWLDKNLESHNYFFIFGGGTRHCPGKELGIA 420

Query: 421 EISTFLHYFVTKYRWEEVGGDELLKFPRVEAPSGFHIRVSSY 463
           EISTFLHYFVTKYRWEEVGGD+LLKFPRVEAPSGFHIRVSSY
Sbjct: 421 EISTFLHYFVTKYRWEEVGGDKLLKFPRVEAPSGFHIRVSSY 462

[Close]

BLAST of Moc04g37060 vs. TAIR 10
Match: AT3G30180.1 (brassinosteroid-6-oxidase 2 )

HSP 1 Score: 686.0 bits (1769), Expect = 2.0e-197
Identity = 322/465 (69.25%), Postives = 392/465 (84.30%), Query Frame = 0


Query: 1   MAVAMAILA-LILLLCFCSALLRWNEVRFSKKGLPPGTMGWPVFGETTEFLKQGPNFMKT 60
           M + M IL  L++++C C+ALLRWN++R+SKKGLPPGTMGWP+FGETTEFLKQGP+FMK 
Sbjct: 1   MGIMMMILGLLVIIVCLCTALLRWNQMRYSKKGLPPGTMGWPIFGETTEFLKQGPDFMKN 60

Query: 61  QRARYGSIFKSHILGCPTVVSMDPEVNRYVLMNESKGLVPGYPQSMLDILGKCNIAAVHG 120
           QR RYGS FKSHILGCPT+VSMD E+NRY+LMNESKGLV GYPQSMLDILG CNIAAVHG
Sbjct: 61  QRLRYGSFFKSHILGCPTIVSMDAELNRYILMNESKGLVAGYPQSMLDILGTCNIAAVHG 120

Query: 121 GAHKLMRAALLAIVSPAMIKDRLLPKVDDFMRSHLRNW-DRQIIDIQDKTKEMALRSSLK 180
            +H+LMR +LL+++SP M+KD LLPK+DDFMR++L  W D + +DIQ+KTK MA  SSL 
Sbjct: 121 PSHRLMRGSLLSLISPTMMKDHLLPKIDDFMRNYLCGWDDLETVDIQEKTKHMAFLSSLL 180

Query: 181 QIAGIESGPLSESFIPEFFKLVLGTLSLPIDLPGTNYRRGIQARKNIIKLLQQLINERRA 240
           QIA     P  E +  EFFKLV+GTLS+PID+PGTNYR G+QAR NI +LL +L+ ER+ 
Sbjct: 181 QIAETLKKPEVEEYRTEFFKLVVGTLSVPIDIPGTNYRSGVQARNNIDRLLTELMQERKE 240

Query: 241 SKTEKDDMLGFFM-SEENKYKLKDEEIIDQIITVLYSGYETVSTTSMMAVKFLHDHPKVL 300
           S     DMLG+ M  E+N+Y L D+EI DQ++T+LYSGYETVSTTSMMA+K+LHDHPK L
Sbjct: 241 SGETFTDMLGYLMKKEDNRYLLTDKEIRDQVVTILYSGYETVSTTSMMALKYLHDHPKAL 300

Query: 301 QQLREEHMAIRERKNPEDPIDWDDLKAMKFTRAVIFETSRLATIVNGVLRKTTKDMELNG 360
           ++LR EH+AIRERK P++P+  DD+K+MKFTRAVIFETSRLATIVNGVLRKTT D+ELNG
Sbjct: 301 EELRREHLAIRERKRPDEPLTLDDIKSMKFTRAVIFETSRLATIVNGVLRKTTHDLELNG 360

Query: 361 FLIPKGWRIYVYTREINYDPFLYSEPFTFNPWRWLDKNLESHNYFFIFGGGSRLCPGKEL 420
           +LIPKGWRIYVYTREINYD  LY +P  FNPWRW++K+LES +YF +FGGG RLCPGKEL
Sbjct: 361 YLIPKGWRIYVYTREINYDTSLYEDPMIFNPWRWMEKSLESKSYFLLFGGGVRLCPGKEL 420

Query: 421 GVAEISTFLHYFVTKYRWEEVGGDELLKFPRVEAPSGFHIRVSSY 463
           G++E+S+FLHYFVTKYRWEE G D+L+ FPRV AP G+H++ S Y
Sbjct: 421 GISEVSSFLHYFVTKYRWEENGEDKLMVFPRVSAPKGYHLKCSPY 465

[Close]

BLAST of Moc04g37060 vs. TAIR 10
Match: AT5G38970.1 (brassinosteroid-6-oxidase 1 )

HSP 1 Score: 681.0 bits (1756), Expect = 6.6e-196
Identity = 321/465 (69.03%), Postives = 389/465 (83.66%), Query Frame = 0


Query: 1   MAVAMAILALILLL-CFCSALLRWNEVRFSKKGLPPGTMGWPVFGETTEFLKQGPNFMKT 60
           M   M ++ L+L++   CSALLRWN++R++K GLPPGTMGWP+FGETTEFLKQGPNFM+ 
Sbjct: 1   MGAMMVMMGLLLIIVSLCSALLRWNQMRYTKNGLPPGTMGWPIFGETTEFLKQGPNFMRN 60

Query: 61  QRARYGSIFKSHILGCPTVVSMDPEVNRYVLMNESKGLVPGYPQSMLDILGKCNIAAVHG 120
           QR RYGS FKSH+LGCPT++SMD EVNRY+L NESKGLVPGYPQSMLDILG CN+AAVHG
Sbjct: 61  QRLRYGSFFKSHLLGCPTLISMDSEVNRYILKNESKGLVPGYPQSMLDILGTCNMAAVHG 120

Query: 121 GAHKLMRAALLAIVSPAMIKDRLLPKVDDFMRSHLRNWDR-QIIDIQDKTKEMALRSSLK 180
            +H+LMR +LL+++S  M++D +LPKVD FMRS+L  W+  ++IDIQDKTK MA  SSL 
Sbjct: 121 SSHRLMRGSLLSLISSTMMRDHILPKVDHFMRSYLDQWNELEVIDIQDKTKHMAFLSSLT 180

Query: 181 QIAGIESGPLSESFIPEFFKLVLGTLSLPIDLPGTNYRRGIQARKNIIKLLQQLINERRA 240
           QIAG    P  E F   FFKLV+GTLS+PIDLPGTNYR GIQAR NI +LL++L+ ERR 
Sbjct: 181 QIAGNLRKPFVEEFKTAFFKLVVGTLSVPIDLPGTNYRCGIQARNNIDRLLRELMQERRD 240

Query: 241 SKTEKDDMLGFFMSEE-NKYKLKDEEIIDQIITVLYSGYETVSTTSMMAVKFLHDHPKVL 300
           S     DMLG+ M +E N+Y L DEEI DQ++T+LYSGYETVSTTSMMA+K+LHDHPK L
Sbjct: 241 SGETFTDMLGYLMKKEGNRYPLTDEEIRDQVVTILYSGYETVSTTSMMALKYLHDHPKAL 300

Query: 301 QQLREEHMAIRERKNPEDPIDWDDLKAMKFTRAVIFETSRLATIVNGVLRKTTKDMELNG 360
           Q+LR EH+A RERK  ++P+  +D+K+MKFTRAVI+ETSRLATIVNGVLRKTT+D+E+NG
Sbjct: 301 QELRAEHLAFRERKRQDEPLGLEDVKSMKFTRAVIYETSRLATIVNGVLRKTTRDLEING 360

Query: 361 FLIPKGWRIYVYTREINYDPFLYSEPFTFNPWRWLDKNLESHNYFFIFGGGSRLCPGKEL 420
           +LIPKGWRIYVYTREINYD  LY +P  FNPWRW+ K+LES N  F+FGGG+RLCPGKEL
Sbjct: 361 YLIPKGWRIYVYTREINYDANLYEDPLIFNPWRWMKKSLESQNSCFVFGGGTRLCPGKEL 420

Query: 421 GVAEISTFLHYFVTKYRWEEVGGDELLKFPRVEAPSGFHIRVSSY 463
           G+ EIS+FLHYFVT+YRWEE+GGDEL+ FPRV AP GFH+R+S Y
Sbjct: 421 GIVEISSFLHYFVTRYRWEEIGGDELMVFPRVFAPKGFHLRISPY 465

[Close]

BLAST of Moc04g37060 vs. TAIR 10
Match: AT5G38970.3 (brassinosteroid-6-oxidase 1 )

HSP 1 Score: 561.6 bits (1446), Expect = 5.8e-160
Identity = 270/395 (68.35%), Postives = 328/395 (83.04%), Query Frame = 0


Query: 1   MAVAMAILALILLL-CFCSALLRWNEVRFSKKGLPPGTMGWPVFGETTEFLKQGPNFMKT 60
           M   M ++ L+L++   CSALLRWN++R++K GLPPGTMGWP+FGETTEFLKQGPNFM+ 
Sbjct: 1   MGAMMVMMGLLLIIVSLCSALLRWNQMRYTKNGLPPGTMGWPIFGETTEFLKQGPNFMRN 60

Query: 61  QRARYGSIFKSHILGCPTVVSMDPEVNRYVLMNESKGLVPGYPQSMLDILGKCNIAAVHG 120
           QR RYGS FKSH+LGCPT++SMD EVNRY+L NESKGLVPGYPQSMLDILG CN+AAVHG
Sbjct: 61  QRLRYGSFFKSHLLGCPTLISMDSEVNRYILKNESKGLVPGYPQSMLDILGTCNMAAVHG 120

Query: 121 GAHKLMRAALLAIVSPAMIKDRLLPKVDDFMRSHLRNWDR-QIIDIQDKTKEMALRSSLK 180
            +H+LMR +LL+++S  M++D +LPKVD FMRS+L  W+  ++IDIQDKTK MA  SSL 
Sbjct: 121 SSHRLMRGSLLSLISSTMMRDHILPKVDHFMRSYLDQWNELEVIDIQDKTKHMAFLSSLT 180

Query: 181 QIAGIESGPLSESFIPEFFKLVLGTLSLPIDLPGTNYRRGIQARKNIIKLLQQLINERRA 240
           QIAG    P  E F   FFKLV+GTLS+PIDLPGTNYR GIQAR NI +LL++L+ ERR 
Sbjct: 181 QIAGNLRKPFVEEFKTAFFKLVVGTLSVPIDLPGTNYRCGIQARNNIDRLLRELMQERRD 240

Query: 241 SKTEKDDMLGFFMSEE-NKYKLKDEEIIDQIITVLYSGYETVSTTSMMAVKFLHDHPKVL 300
           S     DMLG+ M +E N+Y L DEEI DQ++T+LYSGYETVSTTSMMA+K+LHDHPK L
Sbjct: 241 SGETFTDMLGYLMKKEGNRYPLTDEEIRDQVVTILYSGYETVSTTSMMALKYLHDHPKAL 300

Query: 301 QQLREEHMAIRERKNPEDPIDWDDLKAMKFTRAVIFETSRLATIVNGVLRKTTKDMELNG 360
           Q+LR EH+A RERK  ++P+  +D+K+MKFTRAVI+ETSRLATIVNGVLRKTT+D+E+NG
Sbjct: 301 QELRAEHLAFRERKRQDEPLGLEDVKSMKFTRAVIYETSRLATIVNGVLRKTTRDLEING 360

Query: 361 FLIPKGWRIYVYTREINYDPFLYSEPFTFNPWRWL 393
           +LIPKGWRIYVYTREINYD  LY +P  FNPWRW+
Sbjct: 361 YLIPKGWRIYVYTREINYDANLYEDPLIFNPWRWM 395

[Close]

BLAST of Moc04g37060 vs. TAIR 10
Match: AT5G38970.2 (brassinosteroid-6-oxidase 1 )

HSP 1 Score: 558.9 bits (1439), Expect = 3.8e-159
Identity = 266/384 (69.27%), Postives = 320/384 (83.33%), Query Frame = 0


Query: 81  MDPEVNRYVLMNESKGLVPGYPQSMLDILGKCNIAAVHGGAHKLMRAALLAIVSPAMIKD 140
           MD EVNRY+L NESKGLVPGYPQSMLDILG CN+AAVHG +H+LMR +LL+++S  M++D
Sbjct: 1   MDSEVNRYILKNESKGLVPGYPQSMLDILGTCNMAAVHGSSHRLMRGSLLSLISSTMMRD 60

Query: 141 RLLPKVDDFMRSHLRNWDR-QIIDIQDKTKEMALRSSLKQIAGIESGPLSESFIPEFFKL 200
            +LPKVD FMRS+L  W+  ++IDIQDKTK MA  SSL QIAG    P  E F   FFKL
Sbjct: 61  HILPKVDHFMRSYLDQWNELEVIDIQDKTKHMAFLSSLTQIAGNLRKPFVEEFKTAFFKL 120

Query: 201 VLGTLSLPIDLPGTNYRRGIQARKNIIKLLQQLINERRASKTEKDDMLGFFMSEE-NKYK 260
           V+GTLS+PIDLPGTNYR GIQAR NI +LL++L+ ERR S     DMLG+ M +E N+Y 
Sbjct: 121 VVGTLSVPIDLPGTNYRCGIQARNNIDRLLRELMQERRDSGETFTDMLGYLMKKEGNRYP 180

Query: 261 LKDEEIIDQIITVLYSGYETVSTTSMMAVKFLHDHPKVLQQLREEHMAIRERKNPEDPID 320
           L DEEI DQ++T+LYSGYETVSTTSMMA+K+LHDHPK LQ+LR EH+A RERK  ++P+ 
Sbjct: 181 LTDEEIRDQVVTILYSGYETVSTTSMMALKYLHDHPKALQELRAEHLAFRERKRQDEPLG 240

Query: 321 WDDLKAMKFTRAVIFETSRLATIVNGVLRKTTKDMELNGFLIPKGWRIYVYTREINYDPF 380
            +D+K+MKFTRAVI+ETSRLATIVNGVLRKTT+D+E+NG+LIPKGWRIYVYTREINYD  
Sbjct: 241 LEDVKSMKFTRAVIYETSRLATIVNGVLRKTTRDLEINGYLIPKGWRIYVYTREINYDAN 300

Query: 381 LYSEPFTFNPWRWLDKNLESHNYFFIFGGGSRLCPGKELGVAEISTFLHYFVTKYRWEEV 440
           LY +P  FNPWRW+ K+LES N  F+FGGG+RLCPGKELG+ EIS+FLHYFVT+YRWEE+
Sbjct: 301 LYEDPLIFNPWRWMKKSLESQNSCFVFGGGTRLCPGKELGIVEISSFLHYFVTRYRWEEI 360

Query: 441 GGDELLKFPRVEAPSGFHIRVSSY 463
           GGDEL+ FPRV AP GFH+R+S Y
Sbjct: 361 GGDELMVFPRVFAPKGFHLRISPY 384

[Close]

BLAST of Moc04g37060 vs. TAIR 10
Match: AT5G05690.1 (Cytochrome P450 superfamily protein )

HSP 1 Score: 329.7 bits (844), Expect = 3.7e-90
Identity = 175/468 (37.39%), Postives = 273/468 (58.33%), Query Frame = 0


Query: 5   MAILALILLLCFCSA--LLRWNEVRFSKKGLPPGTMGWPVFGETTEFL----KQGPN-FM 64
           MA  A +LLL   +A  LL     R+ + GLPPG++G P+ GET + +     + P  F+
Sbjct: 1   MAFTAFLLLLSSIAAGFLLLLRRTRYRRMGLPPGSLGLPLIGETFQLIGAYKTENPEPFI 60

Query: 65  KTQRARYGSIFKSHILGCPTVVSMDPEVNRYVLMNESKGLVPGYPQSMLDILGKCNIAAV 124
             + ARYGS+F +H+ G PT+ S DPE NR+VL NE K     YP S+ ++LGK ++  +
Sbjct: 61  DERVARYGSVFMTHLFGEPTIFSADPETNRFVLQNEGKLFECSYPASICNLLGKHSLLLM 120

Query: 125 HGGAHKLMRAALLAIVSPAMIKDRLLPKVDDFMRSHLRNWDRQIIDIQDKTKEMALRSSL 184
            G  HK M +  ++  + ++IKD L+  +D  +R +L +W  +++ + ++ K++    ++
Sbjct: 121 KGSLHKRMHSLTMSFANSSIIKDHLMLDIDRLVRFNLDSWSSRVL-LMEEAKKITFELTV 180

Query: 185 KQIAGIESGPLSESFIPEFFKLVLGTLSLPIDLPGTNYRRGIQARKNIIKLLQQLINERR 244
           KQ+   + G  SES   E+  ++ G  SLP+ L  T YR+ IQAR+ + + L  ++ +RR
Sbjct: 181 KQLMSFDPGEWSESLRKEYLLVIEGFFSLPLPLFSTTYRKAIQARRKVAEALTVVVMKRR 240

Query: 245 ASKTE----KDDMLGFFMSEENKYKLKDEEIIDQIITVLYSGYETVSTTSMMAVKFLHDH 304
             + E    K DML   ++ ++ +   DEEI+D ++ +L +GYET ST   +AVKFL + 
Sbjct: 241 EEEEEGAERKKDMLAALLAADDGF--SDEEIVDFLVALLVAGYETTSTIMTLAVKFLTET 300

Query: 305 PKVLQQLREEHMAIRERKNPEDPIDWDDLKAMKFTRAVIFETSRLATIVNGVLRKTTKDM 364
           P  L QL+EEH  IR  K+    ++W D K+M FT+ V+ ET R+A I+ GV R+   D+
Sbjct: 301 PLALAQLKEEHEKIRAMKSDSYSLEWSDYKSMPFTQCVVNETLRVANIIGGVFRRAMTDV 360

Query: 365 ELNGFLIPKGWRIYVYTREINYDPFLYSEPFTFNPWRWLDKNLES--HNYFFIFGGGSRL 424
           E+ G+ IPKGW+++   R ++ DP  + +  TFNPWRW   ++ +   N F  FGGG RL
Sbjct: 361 EIKGYKIPKGWKVFSSFRAVHLDPNHFKDARTFNPWRWQSNSVTTGPSNVFTPFGGGPRL 420

Query: 425 CPGKELGVAEISTFLHYFVTKYRWEEVGGDELLKFPRVEAPSGFHIRV 460
           CPG EL    +S FLH  VT + W     D+L+ FP       + I V
Sbjct: 421 CPGYELARVALSVFLHRLVTGFSWVPAEQDKLVFFPTTRTQKRYPIFV 465

The following BLAST results are available for this feature:

BLAST of Moc04g37060 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022134388.1	5.5e-269	100.00	cytochrome P450 85A-like [Momordica charantia]	[more]
XP_038890036.1	3.0e-259	95.45	cytochrome P450 85A-like [Benincasa hispida]	[more]
XP_022939540.1	3.1e-256	93.94	cytochrome P450 85A-like [Cucurbita moschata] >XP_023550225.1 cytochrome P450 85...	[more]
KAG6602538.1	4.1e-256	93.07	Cytochrome P450 85A, partial [Cucurbita argyrosperma subsp. sororia]	[more]
KAG7033215.1	4.1e-256	93.07	Cytochrome P450 85A [Cucurbita argyrosperma subsp. argyrosperma]	[more]

BLAST of Moc04g37060 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q69F95	5.2e-214	76.29	Cytochrome P450 85A OS=Phaseolus vulgaris OX=3885 GN=BA13 PE=3 SV=2	[more]
Q43147	6.2e-207	73.06	Cytochrome P450 85A1 OS=Solanum lycopersicum OX=4081 GN=CYP85A1 PE=2 SV=1	[more]
Q940V4	2.9e-196	69.25	Cytochrome P450 85A2 OS=Arabidopsis thaliana OX=3702 GN=CYP85A2 PE=2 SV=1	[more]
Q9FMA5	9.3e-195	69.03	Cytochrome P450 85A1 OS=Arabidopsis thaliana OX=3702 GN=CYP85A1 PE=2 SV=1	[more]
Q50LE0	4.9e-188	66.81	Cytochrome P450 85A3 OS=Solanum lycopersicum OX=4081 GN=CYP85A3 PE=2 SV=1	[more]

BLAST of Moc04g37060 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1C1V2	2.6e-269	100.00	cytochrome P450 85A-like OS=Momordica charantia OX=3673 GN=LOC111006661 PE=3 SV=...	[more]
A0A6J1FN01	1.5e-256	93.94	cytochrome P450 85A-like OS=Cucurbita moschata OX=3662 GN=LOC111445417 PE=3 SV=1	[more]
A0A6J1JUV6	4.4e-256	93.94	cytochrome P450 85A-like OS=Cucurbita maxima OX=3661 GN=LOC111489110 PE=3 SV=1	[more]
A0A6J1GNX1	1.1e-254	92.64	cytochrome P450 85A-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC11145579...	[more]
A0A6J1JRL1	1.4e-254	92.86	cytochrome P450 85A-like OS=Cucurbita maxima OX=3661 GN=LOC111487713 PE=3 SV=1	[more]

BLAST of Moc04g37060 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G30180.1	2.0e-197	69.25	brassinosteroid-6-oxidase 2	[more]
AT5G38970.1	6.6e-196	69.03	brassinosteroid-6-oxidase 1	[more]
AT5G38970.3	5.8e-160	68.35	brassinosteroid-6-oxidase 1	[more]
AT5G38970.2	3.8e-159	69.27	brassinosteroid-6-oxidase 1	[more]
AT5G05690.1	3.7e-90	37.39	Cytochrome P450 superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (OHB3-1) v2
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002401	Cytochrome P450, E-class, group I	PRINTS	PR00463	EP450I	coord: 61..80 score: 29.5 coord: 369..393 score: 29.21 coord: 412..435 score: 23.42 coord: 283..309 score: 20.46 coord: 329..347 score: 30.9 coord: 402..412 score: 45.42 coord: 263..280 score: 21.6
IPR001128	Cytochrome P450	PRINTS	PR00385	P450	coord: 403..412 score: 47.02 coord: 274..291 score: 29.56 coord: 412..423 score: 31.66 coord: 330..341 score: 41.33
IPR001128	Cytochrome P450	PFAM	PF00067	p450	coord: 34..441 e-value: 1.3E-60 score: 205.5
IPR036396	Cytochrome P450 superfamily	GENE3D	1.10.630.10	Cytochrome P450	coord: 24..459 e-value: 4.8E-104 score: 350.6
IPR036396	Cytochrome P450 superfamily	SUPERFAMILY	48264	Cytochrome P450	coord: 27..442
None	No IPR available	PIRSR	PIRSR000047-1	PIRSR000047-1	coord: 5..440 e-value: 1.6E-20 score: 71.3
None	No IPR available	PANTHER	PTHR24286:SF273	CYTOCHROME P450 FAMILY PROTEIN	coord: 8..460
None	No IPR available	PANTHER	PTHR24286	CYTOCHROME P450 26	coord: 8..460
IPR017972	Cytochrome P450, conserved site	PROSITE	PS00086	CYTOCHROME_P450	coord: 405..414

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Moc04g37060.1	Moc04g37060.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0016132	brassinosteroid biosynthetic process
biological_process	GO:0010268	brassinosteroid homeostasis
biological_process	GO:0016125	sterol metabolic process
molecular_function	GO:0020037	heme binding
molecular_function	GO:0005506	iron ion binding
molecular_function	GO:0004497	monooxygenase activity
molecular_function	GO:0016705	oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen