Moc04g36960 (gene) Bitter gourd (OHB3-1) v2

BLAST of Moc04g36960 vs. NCBI nr
Match: XP_022133867.1 (malonyl-CoA:anthocyanidin 5-O-glucoside-6''-O-malonyltransferase-like [Momordica charantia])

HSP 1 Score: 939.5 bits (2427), Expect = 1.1e-269
Identity = 470/470 (100.00%), Postives = 470/470 (100.00%), Query Frame = 0

Query: 1   MAENPQASSMAESHSVKILEICKITPSPSSSSASSLSLPTTFLDTFFFQHIPNQRLLFYS 60
           MAENPQASSMAESHSVKILEICKITPSPSSSSASSLSLPTTFLDTFFFQHIPNQRLLFYS
Sbjct: 1   MAENPQASSMAESHSVKILEICKITPSPSSSSASSLSLPTTFLDTFFFQHIPNQRLLFYS 60

Query: 61  LSSSDPPFLHSLLPQLKLSLSLTLSHFLPLAGNLAWPEISPDPLILYNPGDSVFLTVAQT 120
           LSSSDPPFLHSLLPQLKLSLSLTLSHFLPLAGNLAWPEISPDPLILYNPGDSVFLTVAQT
Sbjct: 61  LSSSDPPFLHSLLPQLKLSLSLTLSHFLPLAGNLAWPEISPDPLILYNPGDSVFLTVAQT 120

Query: 121 DADFHRLSGNQPRPAIESHPLLPELPATNDAALAPAMAIQLTLFPENGFSIGITTDHATH 180
           DADFHRLSGNQPRPAIESHPLLPELPATNDAALAPAMAIQLTLFPENGFSIGITTDHATH
Sbjct: 121 DADFHRLSGNQPRPAIESHPLLPELPATNDAALAPAMAIQLTLFPENGFSIGITTDHATH 180

Query: 181 DGKSSTMFLKAWAFICKLGEGNQSPSLPKELSPVFDRTAIKDYPALDRLYRGYLQRLGRS 240
           DGKSSTMFLKAWAFICKLGEGNQSPSLPKELSPVFDRTAIKDYPALDRLYRGYLQRLGRS
Sbjct: 181 DGKSSTMFLKAWAFICKLGEGNQSPSLPKELSPVFDRTAIKDYPALDRLYRGYLQRLGRS 240

Query: 241 GDVKGIRFKPNNGVTPKMAKATFELTSLDVENLKKKVEKWVPGIRLSTFVLTYAYVSSCL 300
           GDVKGIRFKPNNGVTPKMAKATFELTSLDVENLKKKVEKWVPGIRLSTFVLTYAYVSSCL
Sbjct: 241 GDVKGIRFKPNNGVTPKMAKATFELTSLDVENLKKKVEKWVPGIRLSTFVLTYAYVSSCL 300

Query: 301 VKAEGLDESKKVCLATFADWRPRLDIGIAPNYLGNGGGPIGVFTGVSDFIDKNGIAIVAK 360
           VKAEGLDESKKVCLATFADWRPRLDIGIAPNYLGNGGGPIGVFTGVSDFIDKNGIAIVAK
Sbjct: 301 VKAEGLDESKKVCLATFADWRPRLDIGIAPNYLGNGGGPIGVFTGVSDFIDKNGIAIVAK 360

Query: 361 KIREGITELENAVTEGKMVELIMHSIGKYMKEGPVAKSVSVFGSPRFGLYEVDFGWGRPE 420
           KIREGITELENAVTEGKMVELIMHSIGKYMKEGPVAKSVSVFGSPRFGLYEVDFGWGRPE
Sbjct: 361 KIREGITELENAVTEGKMVELIMHSIGKYMKEGPVAKSVSVFGSPRFGLYEVDFGWGRPE 420

Query: 421 KVEFPSVDGGNLISLAESRDGSGGVEVGVALPTETMQVFGSLFSEGLRSL 471
           KVEFPSVDGGNLISLAESRDGSGGVEVGVALPTETMQVFGSLFSEGLRSL
Sbjct: 421 KVEFPSVDGGNLISLAESRDGSGGVEVGVALPTETMQVFGSLFSEGLRSL 470

BLAST of Moc04g36960 vs. NCBI nr
Match: XP_023512232.1 (malonyl-CoA:anthocyanidin 5-O-glucoside-6''-O-malonyltransferase-like, partial [Cucurbita pepo subsp. pepo])

HSP 1 Score: 427.9 bits (1099), Expect = 1.1e-115
Identity = 221/458 (48.25%), Postives = 304/458 (66.38%), Query Frame = 0

Query: 16  VKILEICKITPSPSSSSASSLSLPTTFLDTFFFQHIPNQRLLFYSLSSSDPPFLHSLLPQ 75
           ++++E+C +TPSP  S  +  SLP TF D   F   P +R+LFY+L ++D P   S+L  
Sbjct: 8   MEVMEVCNVTPSP--SPLTPFSLPLTFFDLVQFLAPPTERILFYTLPANDLPRFDSILQT 67

Query: 76  LKLSLSLTLSHFLPLAGNLAWPEISPDPLILYNPGDSVFLTVAQTDADFHRLSGNQPRPA 135
           LK SLS TLSHFLPLAGNL WP  SP+PLI+YNPGDSV LT+A+T+ADFH LS N  R A
Sbjct: 68  LKHSLSHTLSHFLPLAGNLVWPHHSPEPLIVYNPGDSVSLTIAKTEADFHHLSSNHVRKA 127

Query: 136 IESHPLLPELPATNDAALAPAMAIQLTLFPENGFSIGITTDHATHDGKSSTMFLKAWAFI 195
            ESH L+P+LP ++   +AP M++Q+TLFP++GFSIGI T+H   DGK+STMFLK+WA I
Sbjct: 128 KESHFLVPQLPTSD--TIAPTMSLQITLFPKSGFSIGIVTNHVVVDGKTSTMFLKSWASI 187

Query: 196 CK----LGEGNQSPSLPKELSPVFDRTAIKDYPALDRLYRGYLQRLGRSGDVKGIRFKPN 255
           C         N  P+LP EL+P FDRTA +D   L RLY  Y     R   + G+   P 
Sbjct: 188 CSTLNCTTNNNNLPTLPSELTPCFDRTAARDPNGLQRLYVKYFGMFVRQHKILGL--TPK 247

Query: 256 NGVTPKMAKATFELTSLDVENLKKKVEKWVPGIR-LSTFVLTYAYVSSCLVKAEGLDESK 315
             ++  +  ATFELT +D+E L++KV       R L+TFVLT++ +S+C+VKAE +++  
Sbjct: 248 RVISNDVVYATFELTRIDIEKLRRKVATNSTSTRYLTTFVLTFSLISTCIVKAERIEQKS 307

Query: 316 KVCLATFADWRPRLDIGIAPNYLGNGGGPIGVFTGVSDFIDKNGIAIVAKKIREGITELE 375
           K+ L    D RPR+      NY GNG   +GVF    +  ++NG+A+VA KI + + E+E
Sbjct: 308 KIALFFAMDMRPRMGALGGLNYFGNGVSTLGVFLEAKELEEENGMAMVANKISDRMMEIE 367

Query: 376 NAVTEGKMVELIMHSIGKYMKEGPVAKSVSVFGSPRFGLYEVDFGWGRPEKVEFPSVDGG 435
            +V E K+VE++   +G + +E P++K + V GSPRFG+YE+DFGWG  +KVE  S+D  
Sbjct: 368 RSVKENKVVEMVEEILGGWGREMPISKMIGVGGSPRFGVYEIDFGWGNSKKVEMASMDPN 427

Query: 436 NLISLAESRDGSGGVEVGVALPTETMQVFGSLFSEGLR 469
              S+ ESR G+GGVEVG+ALP + M VF SLF E ++
Sbjct: 428 GTFSMTESRSGNGGVEVGIALPNDVMNVFSSLFYEEVK 459

BLAST of Moc04g36960 vs. NCBI nr
Match: KAG6571181.1 (Malonyl-CoA:anthocyanidin 5-O-glucoside-6''-O-malonyltransferase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 424.1 bits (1089), Expect = 1.6e-114
Identity = 222/458 (48.47%), Postives = 302/458 (65.94%), Query Frame = 0

Query: 16  VKILEICKITPSPSSSSASSLSLPTTFLDTFFFQHIPNQRLLFYSLSSSDPPFLHSLLPQ 75
           +++LE+C ITPSP  S  +  SLP TF D   F   P +R+LFY+L ++D P   S+L  
Sbjct: 1   MEVLEVCNITPSP--SPPTPFSLPLTFFDLVQFLAPPTERILFYTLPANDLPRFDSILQT 60

Query: 76  LKLSLSLTLSHFLPLAGNLAWPEISPDPLILYNPGDSVFLTVAQTDADFHRLSGNQPRPA 135
           LK SLS TLSHFLPLAGNL  P  SP+PLI+YNPGDSV LT+A+T+ADFH LS N  R A
Sbjct: 61  LKHSLSHTLSHFLPLAGNLVLPHHSPEPLIVYNPGDSVSLTIAKTEADFHHLSSNHVRKA 120

Query: 136 IESHPLLPELPATNDAALAPAMAIQLTLFPENGFSIGITTDHATHDGKSSTMFLKAWAFI 195
            ESH L+P+LP ++   +AP M++Q+TLFP++GFSIGI T+H   DGK+STMFLK+WA I
Sbjct: 121 TESHFLVPQLPTSD--TIAPPMSLQITLFPKSGFSIGIVTNHVVVDGKTSTMFLKSWASI 180

Query: 196 C---KLGEGNQSPSLPKELSPVFDRTAIKDYPALDRLYRGYLQRLGRSGDVKGIRFKPNN 255
           C        N  P+LP EL+P FDRTA +D   L RLY  Y     R   + G+   P  
Sbjct: 181 CSTLNCTNNNNLPTLPPELTPFFDRTAARDPNGLQRLYVKYYGAFVRQPKILGL--TPKR 240

Query: 256 GVTPKMAKATFELTSLDVENLKKKVEKWVPGI--RLSTFVLTYAYVSSCLVKAEGLDESK 315
            ++  +  ATFELT +D+E L+ KV         RLSTF+L ++ +S+C+VKAE ++   
Sbjct: 241 VISDDVVYATFELTRIDIEKLRTKVVATSSSTPRRLSTFMLAFSLISTCIVKAERIEPKS 300

Query: 316 KVCLATFADWRPRLDIGIAPNYLGNGGGPIGVFTGVSDFIDKNGIAIVAKKIREGITELE 375
           K+ L    D RPR+      NY GNG   +GVF    +  ++NG+A+VA KI + + E+E
Sbjct: 301 KIALFFAMDMRPRMGALGGLNYFGNGVSTLGVFLEAKELEEENGMAMVANKISDRMMEIE 360

Query: 376 NAVTEGKMVELIMHSIGKYMKEGPVAKSVSVFGSPRFGLYEVDFGWGRPEKVEFPSVDGG 435
           N+V E K+VE++   +G++ +E P++K + V GSPRFG+YE+DFGWG  +KVE  S+D  
Sbjct: 361 NSVKENKVVEMVEEILGRWGREMPISKMIGVGGSPRFGVYEIDFGWGNSKKVEMASMDPN 420

Query: 436 NLISLAESRDGSGGVEVGVALPTETMQVFGSLFSEGLR 469
              S+ ESR G+GGVEVG+ALP + M VF SLF E ++
Sbjct: 421 GTFSITESRSGNGGVEVGIALPNDVMNVFSSLFYEEVK 452

BLAST of Moc04g36960 vs. NCBI nr
Match: XP_022944048.1 (malonyl-CoA:anthocyanidin 5-O-glucoside-6''-O-malonyltransferase-like [Cucurbita moschata])

HSP 1 Score: 421.8 bits (1083), Expect = 7.9e-114
Identity = 222/457 (48.58%), Postives = 299/457 (65.43%), Query Frame = 0

Query: 16  VKILEICKITPSPSSSSASSLSLPTTFLDTFFFQHIPNQRLLFYSLSSSDPPFLHSLLPQ 75
           +++LE+C ITPSP  S  +  SLP TF D   F   P +R+LFY+L ++D P   S+L  
Sbjct: 22  MEVLEVCNITPSP--SPPTHFSLPLTFFDLVQFLAPPTERILFYTLPANDLPRFDSILQT 81

Query: 76  LKLSLSLTLSHFLPLAGNLAWPEISPDPLILYNPGDSVFLTVAQTDADFHRLSGNQPRPA 135
           LK SLSLTLSHFLPLAGNL WP  S DP ILYNPGDSV LT+A+T+ADFH LS N  R A
Sbjct: 82  LKHSLSLTLSHFLPLAGNLVWPHHSQDPFILYNPGDSVSLTIAKTEADFHHLSSNHVRKA 141

Query: 136 IESHPLLPELPATNDAALAPAMAIQLTLFPENGFSIGITTDHATHDGKSSTMFLKAWAFI 195
            ESH L+P+LP ++   +AP M++Q+TLFP++GFSIGI T+H   DGK+STMFLK+WA I
Sbjct: 142 KESHFLVPQLPTSD--TIAPPMSLQITLFPKSGFSIGIVTNHVVVDGKTSTMFLKSWASI 201

Query: 196 CKL--GEGNQSPSLPKELSPVFDRTAIKDYPALDRLYRGYLQRLGRSGDVKGIRFKPNNG 255
           C       N  P+LP EL+P FDRTA +D   L RLY  Y     R   + G+   P   
Sbjct: 202 CSTLNCTNNNLPTLPPELTPFFDRTAARDPNGLQRLYVKYYGAFVRQPKILGL--TPKRV 261

Query: 256 VTPKMAKATFELTSLDVENLKKKVEKWVPGI--RLSTFVLTYAYVSSCLVKAEGLDESKK 315
           ++  +  ATFELT  D+E L+ KV         RLSTF+L ++ +S+C+VKAE ++   K
Sbjct: 262 ISDDVVYATFELTRTDIEKLRTKVVATSSSTPRRLSTFMLAFSLISTCIVKAERIEPKSK 321

Query: 316 VCLATFADWRPRLDIGIAPNYLGNGGGPIGVFTGVSDFIDKNGIAIVAKKIREGITELEN 375
           + L    D RPR+      NY GN    +GVF    +  ++NG+A+VA KI + + E+EN
Sbjct: 322 IALFFAMDMRPRMGALGGLNYFGNCVSTLGVFLEAKELEEENGMAMVANKISDRMMEIEN 381

Query: 376 AVTEGKMVELIMHSIGKYMKEGPVAKSVSVFGSPRFGLYEVDFGWGRPEKVEFPSVDGGN 435
           +V E K+VE++   +G++ +E P++K + V GSPRFG+YE+DFGWG  +KVE  S+D   
Sbjct: 382 SVKENKVVEMVEEILGRWGREMPISKMIGVGGSPRFGVYEIDFGWGNSKKVEMASMDPNG 441

Query: 436 LISLAESRDGSGGVEVGVALPTETMQVFGSLFSEGLR 469
             S+ ESR G+GGVEVG+ALP + M V  SLF E ++
Sbjct: 442 TFSITESRSGNGGVEVGIALPNDVMNVLSSLFYEEVK 472

BLAST of Moc04g36960 vs. NCBI nr
Match: KAG7010988.1 (Malonyl-CoA:anthocyanidin 5-O-glucoside-6''-O-malonyltransferase, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 416.4 bits (1069), Expect = 3.3e-112
Identity = 221/457 (48.36%), Postives = 300/457 (65.65%), Query Frame = 0

Query: 16  VKILEICKITPSPSSSSASSLSLPTTFLDTFFFQHIPNQRLLFYSLSSSDPPFLHSLLPQ 75
           +++LE+C IT  PS+S  +  SLP TF D   F   P +R+LFYSL ++D P   S+L  
Sbjct: 1   MEVLEVCNIT--PSTSPPTHFSLPLTFSDLVQFMSPPTERILFYSLPANDLPRFDSILQT 60

Query: 76  LKLSLSLTLSHFLPLAGNLAWPEISPDPLILYNPGDSVFLTVAQTDADFHRLSGNQPRPA 135
           LK SLSLTLSHFLPLAGNL WP+ SP+P I+YNPGDSV LT+A+T+ADF  L  N  R A
Sbjct: 61  LKHSLSLTLSHFLPLAGNLVWPQHSPEPFIVYNPGDSVSLTIAKTEADFDHLLSNHVRKA 120

Query: 136 IESHPLLPELPATNDAALAPAMAIQLTLFPENGFSIGITTDHATHDGKSSTMFLKAWAFI 195
            ESH L+P+LP ++   +APAM++Q+TLFP++GFSIGI T H   DGK+STMFLK+WA I
Sbjct: 121 KESHFLVPQLPTSD--TIAPAMSLQITLFPKSGFSIGIITSHVVVDGKTSTMFLKSWASI 180

Query: 196 C---KLGEGNQSPSLPKELSPVFDRTAIKDYPALDRLYRGYLQRLGRSGDVKGIRFKPNN 255
           C        N  P+LP EL+P FDRTA KD   L+RLY  +     R   +  +   P  
Sbjct: 181 CSTLNCTNNNNLPTLPPELTPFFDRTAAKDPNGLERLYVKHYGAFVRQPKI--LVLTPKR 240

Query: 256 GVTPKMAKATFELTSLDVENLKKKVEKWVPGIR-LSTFVLTYAYVSSCLVKAEGLDESKK 315
            ++  +  ATFELT +D+E L++KV       R L+TFVLTYA +S+C+VKAE ++   K
Sbjct: 241 VISDDVVHATFELTRIDIEKLRRKVATNSTSTRHLTTFVLTYALISTCIVKAERIEPKSK 300

Query: 316 VCLATFADWRPRLDIGIAPNYLGNGGGPIGVFTGVSDFIDKNGIAIVAKKIREGITELEN 375
           + L    D RPR+      NY GNG   +G F    +  ++NG+A+VA KI + I E+E 
Sbjct: 301 IALFFAVDMRPRMGALGGLNYFGNGVIGLGEFLEAKELEEENGMAMVANKISDRIMEMEK 360

Query: 376 AVTEGKMVELIMHSIGKYMKEGPVAKSVSVFGSPRFGLYEVDFGWGRPEKVEFPSVDGGN 435
           +V E K+VE++   +G++ +E P++K +   GSPRFG+YE+DFGWG  +KVE  S++   
Sbjct: 361 SVKENKVVEMVEEILGRWGREMPISKMIVAGGSPRFGVYEIDFGWGNSKKVEMVSINPNG 420

Query: 436 LISLAESRDGSGGVEVGVALPTETMQVFGSLFSEGLR 469
             S+ ESR G+GGVEVGV LP + M VF SLF E ++
Sbjct: 421 NFSMTESRSGNGGVEVGVILPKDVMNVFSSLFYEEVK 451

BLAST of Moc04g36960 vs. ExPASy Swiss-Prot
Match: Q940Z5 (Phenolic glucoside malonyltransferase 1 OS=Arabidopsis thaliana OX=3702 GN=PMAT1 PE=1 SV=1)

HSP 1 Score: 305.4 bits (781), Expect = 1.1e-81
Identity = 179/473 (37.84%), Postives = 270/473 (57.08%), Query Frame = 0

Query: 15  SVKILEICKITPSPSSSSASSLSLPTTFLDTFFFQHIPNQRLLFYSLSSSDPPFLHS-LL 74
           S+K++++ ++TPS +S S+ SL+LP TF D  +++    +R++FY L+ +  PF  S ++
Sbjct: 9   SLKVIDVARVTPS-NSDSSESLTLPLTFFDLLWYKLHAVERVIFYKLTDASRPFFDSVIV 68

Query: 75  PQLKLSLSLTLSHFLPLAGNLAWPEISPDPLILYNPGDSVFLTVAQTDADFHRLSGNQPR 134
           P LK SLS +LSH+LPLAG L W  + P P I+Y P D+V  TVA+++ADF RL+G +P 
Sbjct: 69  PNLKTSLSSSLSHYLPLAGKLVWEPLDPKPKIVYTPNDAVSFTVAESNADFSRLTGKEPF 128

Query: 135 PAIESHPLLPELPATNDAALAPAMAIQLTLFPENGFSIGITTDHATHDGKSSTMFLKAWA 194
           P  E +PL+PEL  ++D+  A A++ Q+TLFP  GF I +   HA  DGK++T FLK+WA
Sbjct: 129 PTTELYPLVPELHVSDDS--ASAVSFQVTLFPNQGFCISVNAHHAVLDGKTTTNFLKSWA 188

Query: 195 FICKLGEGNQSPSLPKELSPVFDRTAIKDYPALDRLYRGYLQRLGR-------SGDVKGI 254
             CK    NQ   LP++L PV+DRT IKD   LD        R+ +         + K +
Sbjct: 189 RTCK----NQDSFLPQDLIPVYDRTVIKDPMDLDTKILNAWHRVAKVFTGGKEPENPKSL 248

Query: 255 RFKPNNGVTPKMAKATFELTSLDVENLKKKVEKWVPG---------IRLSTFVLTYAYVS 314
           +   +  + P + + T  LT  D++ L+++++K             +RLSTFV+ Y+Y  
Sbjct: 249 KLLWSPEIGPDVFRYTLNLTREDIQKLRERLKKESSSSSVSSSPKELRLSTFVIVYSYAL 308

Query: 315 SCLVKAEGLDESKKVCLATFADWRPRLDIGIAPNYLGN-GGGPIGVFTGVSDFIDKNGIA 374
           +CL+KA G D S+ V      D R  +   +  +Y GN       +      F+ + G  
Sbjct: 309 TCLIKARGGDPSRPVGYGFAVDCRSLMVPPVPSSYFGNCVSACFKMSLTAETFMSEEGFL 368

Query: 375 IVAKKIREGITELENAVTEGKMVELIMHSIGKYMKEGPVAKSVSVFGSPRFGLYEVDFGW 434
             A+ + + +  L+  V   K+ E+    +  +    P  + +SV GS RFG+Y +DFGW
Sbjct: 369 AAARMVSDSVEALDENVAL-KIPEI----LEGFTTLSPGTQVLSVAGSTRFGVYGLDFGW 428

Query: 435 GRPEKVEFPSVDGGNLISLAESRDGSGGVEVGVALPTETMQVFGSLFSEGLRS 470
           GRPEKV   S+D G  IS AESRDGSGGVE+G +L    M V   L  +GL +
Sbjct: 429 GRPEKVVVVSIDQGEAISFAESRDGSGGVELGFSLKKHEMDVLVDLLHKGLEN 469

BLAST of Moc04g36960 vs. ExPASy Swiss-Prot
Match: Q9LJB4 (Malonyl-CoA:anthocyanidin 5-O-glucoside-6''-O-malonyltransferase OS=Arabidopsis thaliana OX=3702 GN=5MAT PE=1 SV=1)

HSP 1 Score: 296.6 bits (758), Expect = 5.0e-79
Identity = 175/457 (38.29%), Postives = 267/457 (58.42%), Query Frame = 0

Query: 15  SVKILEICKITPSPSSSSASSLSLPTTFLDTFFFQHIPNQRLLFYSLSSSDPPFLHSLLP 74
           +V ILE+ +++P     S++SL+LP T+ D  + +  P  R+LFY +         SL+ 
Sbjct: 7   AVNILEVVQVSP----PSSNSLTLPLTYFDLGWLKLHPVDRVLFYHVPELT---RSSLIS 66

Query: 75  QLKLSLSLTLSHFLPLAGNLAWPEISPDPLILYNPG--DSVFLTVAQTDADFHRLSGNQP 134
           +LK SLS TL H+LPLAG L W  I   P I+Y+P   D+V+LTVA+++ D   LSG++P
Sbjct: 67  KLKSSLSATLLHYLPLAGRLVWDSIKTKPSIVYSPDDKDAVYLTVAESNGDLSHLSGDEP 126

Query: 135 RPAIESHPLLPELPATNDAALAPAMAIQLTLFPENGFSIGITTDHATHDGKSSTMFLKAW 194
           RPA E H L+PELP ++++  A  +A+Q+T FP  GFS+G+T  HA  DGK++ MFLKAW
Sbjct: 127 RPATEFHSLVPELPVSDES--ARVLAVQVTFFPNQGFSLGVTAHHAVLDGKTTAMFLKAW 186

Query: 195 AFICKLGEGNQSPSLPKELSPVFDRTAIKDYPALDRLYRGYLQRLGRSGDVKGIRFKPNN 254
           A  CK     +  +LP +L P  DR  ++D   L+       + +  S +   ++  P+ 
Sbjct: 187 AHNCK----QEQEALPHDLVPSLDRIIVQDPTGLETKLLN--RWISASNNKPSLKLFPSK 246

Query: 255 GVTPKMAKATFELTSLDVENLKKKV--EKWVPGIRLSTFVLTYAYVSSCLVKAEGLDESK 314
            +   + + T+ LT  D++ L+++V  E     +RLSTFV+TYAYV +C+VK  G D ++
Sbjct: 247 IIGSDILRVTYRLTREDIKKLRERVETESHAKQLRLSTFVITYAYVITCMVKMRGGDPTR 306

Query: 315 KVCLATFADWRPRLDIGIAPNYLGNGGGPIGVFTGVSDFIDKNGIAIVAKKIREGITELE 374
            VC+   +D+R RL+  + P + GN     G F   ++ I + G         E +T   
Sbjct: 307 FVCVGFASDFRSRLNPPLPPTFFGNCIVGSGDFDVKAEPILEEGEGKGFITAVETLTGWV 366

Query: 375 NAVTEGKMVELIMHSIGKYMKEGPVAKSVSVFGSPRFGLYEVDFGWGRPEKVEFPSVDGG 434
           N +    + + ++     + +  P  + +SV GS R G+Y  DFGWG+P KVE  ++D  
Sbjct: 367 NGLCPENIEKNMLLPFEAFKRMEPGRQMISVAGSTRLGIYGSDFGWGKPVKVEIVTIDKD 426

Query: 435 NLISLAESRDGSGGVEVGVALPTETMQVFGSLFSEGL 468
             +SL+ES DGSGGVEVGV L  + ++ FGSLFS GL
Sbjct: 427 ASVSLSESGDGSGGVEVGVCLKKDDVERFGSLFSIGL 448

BLAST of Moc04g36960 vs. ExPASy Swiss-Prot
Match: Q9LRQ8 (Phenolic glucoside malonyltransferase 2 OS=Arabidopsis thaliana OX=3702 GN=PMAT2 PE=1 SV=1)

HSP 1 Score: 291.2 bits (744), Expect = 2.1e-77
Identity = 170/460 (36.96%), Postives = 263/460 (57.17%), Query Frame = 0

Query: 15  SVKILEICKITPSPSS--SSASSLSLPTTFLDTFFFQHIPNQRLLFYSLSSSDPPFLHS- 74
           ++ ++E  ++TP+  S  +SA+   LP TF D  +    P +R+ FY L+ S     HS 
Sbjct: 2   TLHVIETARVTPTDYSVINSANLHKLPLTFFDLPWLLFQPVKRVFFYELTESTRDHFHSI 61

Query: 75  LLPQLKLSLSLTLSHFLPLAGNLAWPEISPDPLILYNPGDSVFLTVAQTDADFHRLSGNQ 134
           +LP+LK SLSL L ++LPL G++ W    P P I+ +    V +T+A++DADF  LSG  
Sbjct: 62  ILPKLKDSLSLILRNYLPLTGHITWEPNEPKPSIIVSENGVVLVTIAESDADFSHLSGYG 121

Query: 135 PRPAIESHPLLPELPATNDAALAPAMAIQLTLFPENGFSIGITTDHATHDGKSSTMFLKA 194
            RP  E H L+P+LP ++D+  A A +IQ+TLFP  GFSIG+   HA  DGK+S+ F+KA
Sbjct: 122 QRPLSELHALVPKLPVSDDS--ATAFSIQITLFPNQGFSIGVAAHHAVLDGKTSSTFIKA 181

Query: 195 WAFICKLGEGNQSPSLPKELSPVFDRTAIKDYPALDRLYRGYLQRLGR-SGDVKGIRFKP 254
           WA ICK     +  S+P+ L+P +DR+ IK    LD      ++ L     +++ +   P
Sbjct: 182 WAQICK----QELQSMPENLTPSYDRSLIKYPTYLDEKMIELVRSLKEDQTNIRSLTSLP 241

Query: 255 NNGVTPKMAKATFELTSLDVENLKKKVEKWVPGIRLSTFVLTYAYVSSCLVKAEGLDESK 314
           ++ +   +  AT  L+  D+E L+++V+   P + LSTFV+ YAY  +C VKA G ++ +
Sbjct: 242 SSKLGDDVVLATLVLSRADIERLREQVKNVSPSLHLSTFVIAYAYAWTCFVKARGGNKDR 301

Query: 315 KVCLATFADWRPRLDIGIAPNYLGNGGGPIGVFT-GVSDFIDKNGIAIVAKKIREGITEL 374
            V L    D+R RLD  +   Y GN   P+G +    ++F+++ G    A    E I++L
Sbjct: 302 SVSLLFVGDFRDRLDPKLPGTYFGNCMIPVGCYNRKAAEFMEEKGFVTAA----EIISDL 361

Query: 375 ENAVTEGKMVELIMHSIGKYMKEGPVAKSVSVFGSPRFGLYEVDFGWGRPEKVEFPSVDG 434
              ++  K+  +    +  +  +    +  ++ GS R G+YE DFGWGRP KV+  S+D 
Sbjct: 362 VKGLSSRKIETIADTFVEGFSFQSWSTQFGTIAGSTRLGVYEADFGWGRPVKVDIVSIDQ 421

Query: 435 GNLISLAESRDGSGGVEVGVALPTETMQVFGSLFSEGLRS 470
           G  I++AE RD SGGVE+G+ L    M    S F+ GL S
Sbjct: 422 GEAIAMAERRDESGGVEIGMCLKKTEMDSVVSFFNNGLHS 451

BLAST of Moc04g36960 vs. ExPASy Swiss-Prot
Match: Q9LRQ7 (BAHD acyltransferase At3g29680 OS=Arabidopsis thaliana OX=3702 GN=At3g29680 PE=2 SV=1)

HSP 1 Score: 253.8 bits (647), Expect = 3.7e-66
Identity = 170/467 (36.40%), Postives = 253/467 (54.18%), Query Frame = 0

Query: 15  SVKILEICKITPSPSSSSASSLSLPTTFLDTFFFQHIPNQRLLFYSLS-SSDPPFLHSLL 74
           ++ +++I ++  S  ++S   L LP TF D  + +  P +R+ FY L+ SS   F  S+L
Sbjct: 2   ALNVIKISRV--SLVTNSVEPLVLPLTFFDLLWLKLNPIERVTFYKLTESSRDSFFSSIL 61

Query: 75  PQLKLSLSLTLSHFLPLAGNLAWPEISPDPLILYNPGDSVFLTVAQTDADFHRLSGNQPR 134
           P+L+ SLSL LSHFLPL+G+L W    P P I+  P D+V LTV +++ADF  +S  + R
Sbjct: 62  PKLEQSLSLVLSHFLPLSGHLKWNPQDPKPHIVIFPKDTVSLTVVESEADFSYISSKELR 121

Query: 135 PAIESHPLLPELPATNDAALAPAMAIQLTLFPENGFSIGITTDHATHDGKSSTMFLKAWA 194
              E  PL+PEL  ++D+  A  +++Q+TLFP  GFSIG T  H   DGK+++ F K+WA
Sbjct: 122 LETELRPLVPELQVSSDS--ASLLSLQITLFPNQGFSIGTTVHHVVMDGKTASKFHKSWA 181

Query: 195 FICKLGEGNQSPSLPKELSPVFDRTAIKDYPALDRLYRGYLQRLGRSGD-VKGIRFKPNN 254
            ICK G   Q   LP     V DRT I     L++        +    D  + +   P  
Sbjct: 182 HICKHGTTPQDFDLP----TVLDRTVINVPAGLEQKIFQLSSYISEEKDYARTLTLPPAK 241

Query: 255 GVTPKMAKATFELTSLDVENLKK--KVEKWVPGIRLSTFVLTYAYVSSCLVKAEGLDESK 314
            +   + + T ELT +D+E LK+  K E     + LSTFV++YAYV +C+VK+ G D ++
Sbjct: 242 EIDNDVVRVTLELTEVDIEKLKERAKNESTRSDLHLSTFVVSYAYVLTCMVKSCGGDANR 301

Query: 315 KVCLATFADWRPRLDIGIAPNYLGNGGGPIGVFTG--VSDFIDK----NGIAIVAKKIR- 374
            V     AD+R RLD  +   Y GN   PI  F G   + F+ K    NG+ I++  +R 
Sbjct: 302 PVRFMYAADFRNRLDPPVPLTYFGNCVLPID-FNGYKATTFLGKDGYVNGVEILSDSVRG 361

Query: 375 ------EGITELENAVTEGKMVELIMHSIGKYMKEGPVAKSVSVFGSPRFGLYEVDFGWG 434
                 E I E+    T+   ++                ++V+V GS +FG+Y  DFGWG
Sbjct: 362 LGSRNIESIWEVYEDGTKNMKLD---------------TQNVTVTGSNQFGIYGSDFGWG 421

Query: 435 RPEKVEFPSVDGGNLISLAESRDGSGGVEVGVALPTETMQVFGSLFS 465
           RP K +  S+   N  S++  RD  GG+E+G++L    M VF SLF+
Sbjct: 422 RPVKTDVMSLYKNNEFSMSARRDEIGGLEIGISLKKCEMNVFLSLFT 444

BLAST of Moc04g36960 vs. ExPASy Swiss-Prot
Match: Q589Y0 (Phenolic glucoside malonyltransferase 1 OS=Nicotiana tabacum OX=4097 GN=mat1 PE=1 SV=1)

HSP 1 Score: 244.2 bits (622), Expect = 2.9e-63
Identity = 165/469 (35.18%), Postives = 248/469 (52.88%), Query Frame = 0

Query: 18  ILEICKITPSPSSSSASSLSLPTTFLDTFFFQHIPNQRLLFYSLSSSDPPFLHSLLPQLK 77
           ++E C++ PSP   SA+ L+LP T+ D  +      +R+LFY L  S P F+ +++P LK
Sbjct: 4   VIEQCQVVPSP--GSATELTLPLTYFDHVWLAFHRMRRILFYKLPISRPDFVQTIIPTLK 63

Query: 78  LSLSLTLSHFLPLAGNLAWP-EISPDPLILYNPGDSVFLTVAQTDADFHRLSGNQPRPAI 137
            SLSLTL ++LPLAGN+A P + S  P + Y  G+SV +  +++D DF+ L G  PR   
Sbjct: 64  DSLSLTLKYYLPLAGNVACPQDWSGYPELRYVTGNSVSVIFSESDMDFNYLIGYHPRNTK 123

Query: 138 ESHPLLPELPATNDA---ALAPAMAIQLTLFPENGFSIGITTDHATHDGKSSTMFLKAWA 197
           + +  +P+L    DA    LAP +AIQ+TLFP +G SIG T  H   DG +   F++AWA
Sbjct: 124 DFYHFVPQLAEPKDAPGVQLAPVLAIQVTLFPNHGISIGFTNHHVAGDGATIVKFVRAWA 183

Query: 198 FICKLGEGNQSPSLPKELSPVFDRTAIKDYPALDRLYRGYLQRLGRSGDVKGIRFKPNNG 257
            + K G   Q   L  E  P +DR+ IKD   +       +++            K ++ 
Sbjct: 184 LLNKFGGDEQ--FLANEFIPFYDRSVIKDPNGVGMSIWNEMKKYKH-------MMKMSDV 243

Query: 258 VT-PKMAKATFELTSLDVENLKKKVEKWVPGI-RLSTFVLTYAYVSSCLVKAEG-----L 317
           VT P   + TF +T  D+  LK  V    P +  +++F +T AYV +C++K+E      +
Sbjct: 244 VTPPDKVRGTFIITRHDIGKLKNLVLTRRPKLTHVTSFTVTCAYVWTCIIKSEAATGEEI 303

Query: 318 DESKKVCLATFADWRPRLDIGIAPNYLGNGGGPIGVFTGVSDFIDKNGIAIVAKKIREGI 377
           DE+        AD R + +  + P+Y GN        T   D   K G  I  + I E I
Sbjct: 304 DENGMEFFGCAADCRAQFNPPLPPSYFGNALVGYVARTRQVDLAGKEGFTIAVELIGEAI 363

Query: 378 TELENAVTEGKMVELIMHSIGKYMKE---GPVAKSVSVFGSPRFGLYEVDFGWGRPEKVE 437
            +        K  E I+   G + KE       +S+SV GSP+  LY  DFGWGRPEK+E
Sbjct: 364 RK------RMKDEEWILS--GSWFKEYDKVDAKRSLSVAGSPKLDLYAADFGWGRPEKLE 423

Query: 438 FPSVDG--GNLISLAESRDGSGGVEVGVALPTETMQVFGSLFSEGLRSL 471
           F S+D   G  +SL++S+D  G +E+G++L    M  F ++F+ G+  L
Sbjct: 424 FVSIDNDDGISMSLSKSKDSDGDLEIGLSLSKTRMNAFAAMFTHGISFL 453

BLAST of Moc04g36960 vs. ExPASy TrEMBL
Match: A0A6J1BXZ2 (malonyl-CoA:anthocyanidin 5-O-glucoside-6''-O-malonyltransferase-like OS=Momordica charantia OX=3673 GN=LOC111006313 PE=3 SV=1)

HSP 1 Score: 939.5 bits (2427), Expect = 5.4e-270
Identity = 470/470 (100.00%), Postives = 470/470 (100.00%), Query Frame = 0

Query: 1   MAENPQASSMAESHSVKILEICKITPSPSSSSASSLSLPTTFLDTFFFQHIPNQRLLFYS 60
           MAENPQASSMAESHSVKILEICKITPSPSSSSASSLSLPTTFLDTFFFQHIPNQRLLFYS
Sbjct: 1   MAENPQASSMAESHSVKILEICKITPSPSSSSASSLSLPTTFLDTFFFQHIPNQRLLFYS 60

Query: 61  LSSSDPPFLHSLLPQLKLSLSLTLSHFLPLAGNLAWPEISPDPLILYNPGDSVFLTVAQT 120
           LSSSDPPFLHSLLPQLKLSLSLTLSHFLPLAGNLAWPEISPDPLILYNPGDSVFLTVAQT
Sbjct: 61  LSSSDPPFLHSLLPQLKLSLSLTLSHFLPLAGNLAWPEISPDPLILYNPGDSVFLTVAQT 120

Query: 121 DADFHRLSGNQPRPAIESHPLLPELPATNDAALAPAMAIQLTLFPENGFSIGITTDHATH 180
           DADFHRLSGNQPRPAIESHPLLPELPATNDAALAPAMAIQLTLFPENGFSIGITTDHATH
Sbjct: 121 DADFHRLSGNQPRPAIESHPLLPELPATNDAALAPAMAIQLTLFPENGFSIGITTDHATH 180

Query: 181 DGKSSTMFLKAWAFICKLGEGNQSPSLPKELSPVFDRTAIKDYPALDRLYRGYLQRLGRS 240
           DGKSSTMFLKAWAFICKLGEGNQSPSLPKELSPVFDRTAIKDYPALDRLYRGYLQRLGRS
Sbjct: 181 DGKSSTMFLKAWAFICKLGEGNQSPSLPKELSPVFDRTAIKDYPALDRLYRGYLQRLGRS 240

Query: 241 GDVKGIRFKPNNGVTPKMAKATFELTSLDVENLKKKVEKWVPGIRLSTFVLTYAYVSSCL 300
           GDVKGIRFKPNNGVTPKMAKATFELTSLDVENLKKKVEKWVPGIRLSTFVLTYAYVSSCL
Sbjct: 241 GDVKGIRFKPNNGVTPKMAKATFELTSLDVENLKKKVEKWVPGIRLSTFVLTYAYVSSCL 300

Query: 301 VKAEGLDESKKVCLATFADWRPRLDIGIAPNYLGNGGGPIGVFTGVSDFIDKNGIAIVAK 360
           VKAEGLDESKKVCLATFADWRPRLDIGIAPNYLGNGGGPIGVFTGVSDFIDKNGIAIVAK
Sbjct: 301 VKAEGLDESKKVCLATFADWRPRLDIGIAPNYLGNGGGPIGVFTGVSDFIDKNGIAIVAK 360

Query: 361 KIREGITELENAVTEGKMVELIMHSIGKYMKEGPVAKSVSVFGSPRFGLYEVDFGWGRPE 420
           KIREGITELENAVTEGKMVELIMHSIGKYMKEGPVAKSVSVFGSPRFGLYEVDFGWGRPE
Sbjct: 361 KIREGITELENAVTEGKMVELIMHSIGKYMKEGPVAKSVSVFGSPRFGLYEVDFGWGRPE 420

Query: 421 KVEFPSVDGGNLISLAESRDGSGGVEVGVALPTETMQVFGSLFSEGLRSL 471
           KVEFPSVDGGNLISLAESRDGSGGVEVGVALPTETMQVFGSLFSEGLRSL
Sbjct: 421 KVEFPSVDGGNLISLAESRDGSGGVEVGVALPTETMQVFGSLFSEGLRSL 470

BLAST of Moc04g36960 vs. ExPASy TrEMBL
Match: A0A6J1FXM3 (malonyl-CoA:anthocyanidin 5-O-glucoside-6''-O-malonyltransferase-like OS=Cucurbita moschata OX=3662 GN=LOC111448598 PE=3 SV=1)

HSP 1 Score: 421.8 bits (1083), Expect = 3.8e-114
Identity = 222/457 (48.58%), Postives = 299/457 (65.43%), Query Frame = 0

Query: 16  VKILEICKITPSPSSSSASSLSLPTTFLDTFFFQHIPNQRLLFYSLSSSDPPFLHSLLPQ 75
           +++LE+C ITPSP  S  +  SLP TF D   F   P +R+LFY+L ++D P   S+L  
Sbjct: 22  MEVLEVCNITPSP--SPPTHFSLPLTFFDLVQFLAPPTERILFYTLPANDLPRFDSILQT 81

Query: 76  LKLSLSLTLSHFLPLAGNLAWPEISPDPLILYNPGDSVFLTVAQTDADFHRLSGNQPRPA 135
           LK SLSLTLSHFLPLAGNL WP  S DP ILYNPGDSV LT+A+T+ADFH LS N  R A
Sbjct: 82  LKHSLSLTLSHFLPLAGNLVWPHHSQDPFILYNPGDSVSLTIAKTEADFHHLSSNHVRKA 141

Query: 136 IESHPLLPELPATNDAALAPAMAIQLTLFPENGFSIGITTDHATHDGKSSTMFLKAWAFI 195
            ESH L+P+LP ++   +AP M++Q+TLFP++GFSIGI T+H   DGK+STMFLK+WA I
Sbjct: 142 KESHFLVPQLPTSD--TIAPPMSLQITLFPKSGFSIGIVTNHVVVDGKTSTMFLKSWASI 201

Query: 196 CKL--GEGNQSPSLPKELSPVFDRTAIKDYPALDRLYRGYLQRLGRSGDVKGIRFKPNNG 255
           C       N  P+LP EL+P FDRTA +D   L RLY  Y     R   + G+   P   
Sbjct: 202 CSTLNCTNNNLPTLPPELTPFFDRTAARDPNGLQRLYVKYYGAFVRQPKILGL--TPKRV 261

Query: 256 VTPKMAKATFELTSLDVENLKKKVEKWVPGI--RLSTFVLTYAYVSSCLVKAEGLDESKK 315
           ++  +  ATFELT  D+E L+ KV         RLSTF+L ++ +S+C+VKAE ++   K
Sbjct: 262 ISDDVVYATFELTRTDIEKLRTKVVATSSSTPRRLSTFMLAFSLISTCIVKAERIEPKSK 321

Query: 316 VCLATFADWRPRLDIGIAPNYLGNGGGPIGVFTGVSDFIDKNGIAIVAKKIREGITELEN 375
           + L    D RPR+      NY GN    +GVF    +  ++NG+A+VA KI + + E+EN
Sbjct: 322 IALFFAMDMRPRMGALGGLNYFGNCVSTLGVFLEAKELEEENGMAMVANKISDRMMEIEN 381

Query: 376 AVTEGKMVELIMHSIGKYMKEGPVAKSVSVFGSPRFGLYEVDFGWGRPEKVEFPSVDGGN 435
           +V E K+VE++   +G++ +E P++K + V GSPRFG+YE+DFGWG  +KVE  S+D   
Sbjct: 382 SVKENKVVEMVEEILGRWGREMPISKMIGVGGSPRFGVYEIDFGWGNSKKVEMASMDPNG 441

Query: 436 LISLAESRDGSGGVEVGVALPTETMQVFGSLFSEGLR 469
             S+ ESR G+GGVEVG+ALP + M V  SLF E ++
Sbjct: 442 TFSITESRSGNGGVEVGIALPNDVMNVLSSLFYEEVK 472

BLAST of Moc04g36960 vs. ExPASy TrEMBL
Match: A0A6J1J6U6 (malonyl-CoA:anthocyanidin 5-O-glucoside-6''-O-malonyltransferase-like OS=Cucurbita maxima OX=3661 GN=LOC111483993 PE=3 SV=1)

HSP 1 Score: 414.1 bits (1063), Expect = 7.9e-112
Identity = 223/457 (48.80%), Postives = 297/457 (64.99%), Query Frame = 0

Query: 16  VKILEICKITPSPSSSSASSLSLPTTFLDTFFFQHIPNQRLLFYSLSSSDPPFLHSLLPQ 75
           +++LE+C IT  PS+S  +  SLP TF D   F   P +R+LFY+L ++  P   S+L  
Sbjct: 10  MEVLEVCNIT--PSTSPPTHFSLPLTFSDLVQFLSPPTERILFYTLHANHLPRFDSILQT 69

Query: 76  LKLSLSLTLSHFLPLAGNLAWPEISPDPLILYNPGDSVFLTVAQTDADFHRLSGNQPRPA 135
           LK SLSLTLSHFLPLAGNL WP  SPDP ILYN GDSV LT+A+T+ADFH LS N  R A
Sbjct: 70  LKHSLSLTLSHFLPLAGNLVWPHHSPDPFILYNHGDSVSLTIAKTEADFHHLSSNHVRKA 129

Query: 136 IESHPLLPELPATNDAALAPAMAIQLTLFPENGFSIGITTDHATHDGKSSTMFLKAWAFI 195
            ESH L+P+LP ++   +APAM++Q+TLFP++GFSIGI T+H   DGK+STMFLK+WA I
Sbjct: 130 TESHFLVPQLPTSD--TIAPAMSLQITLFPKSGFSIGIITNHVVVDGKTSTMFLKSWASI 189

Query: 196 C---KLGEGNQSPSLPKELSPVFDRTAIKDYPALDRLYRGYLQRLGRSGDVKGIRFKPNN 255
           C        N  P+LP EL+P FDRTA KD   L RLY  +     R   + G+   P  
Sbjct: 190 CSTLNCTNNNNLPTLPSELTPFFDRTAAKDPNGLQRLYVKHYGAFVRQPKILGL--TPKR 249

Query: 256 GVTPKMAKATFELTSLDVENLKKKVEKWVPGIR-LSTFVLTYAYVSSCLVKAEGLDESKK 315
            ++  +  ATFELT +D+E L++KV       R L+TFVLTYA VS+C+V+AE ++   K
Sbjct: 250 VISDDVVYATFELTRIDIEKLRRKVATNSTSTRHLTTFVLTYALVSTCIVQAERIEPKSK 309

Query: 316 VCLATFADWRPRLDIGIAPNYLGNGGGPIGVFTGVSDFIDKNGIAIVAKKIREGITELEN 375
           + L    D RPR+      NY GNG   +G      +  ++NG+A+VA KI + I E+E 
Sbjct: 310 IALFFAVDMRPRMGALGGLNYFGNGVIGLGEILEAKELEEENGMAMVANKISDRIMEMEK 369

Query: 376 AVTEGKMVELIMHSIGKYMKEGPVAKSVSVFGSPRFGLYEVDFGWGRPEKVEFPSVDGGN 435
           +V E K+VE++     ++ +E PV+K +   GSPRFG+YE+DFGWG  +KVE  S+D   
Sbjct: 370 SVKENKVVEMVEEISDRWGREMPVSKMIVAGGSPRFGVYEIDFGWGNSKKVEMASMDPNG 429

Query: 436 LISLAESRDGSGGVEVGVALPTETMQVFGSLFSEGLR 469
             S+ ESR G+GGVEVGV LP + M VF SLF E ++
Sbjct: 430 SFSMTESRSGNGGVEVGVVLPKDVMNVFSSLFYEEVK 460

BLAST of Moc04g36960 vs. ExPASy TrEMBL
Match: A0A6J1FRS6 (malonyl-CoA:anthocyanidin 5-O-glucoside-6''-O-malonyltransferase-like OS=Cucurbita moschata OX=3662 GN=LOC111448225 PE=3 SV=1)

HSP 1 Score: 409.8 bits (1052), Expect = 1.5e-110
Identity = 221/456 (48.46%), Postives = 296/456 (64.91%), Query Frame = 0

Query: 16  VKILEICKITPSPSSSSASSLSLPTTFLDTFFFQHIPNQRLLFYSLSSSDPPFLHSLLPQ 75
           +++LE+C IT  PS+S  +  SLP TF D   F   P +R+LFYSL ++D     S+L  
Sbjct: 9   MEVLEVCNIT--PSTSPPTHFSLPLTFSDLVQFISPPTERILFYSLPANDLLRFDSILQT 68

Query: 76  LKLSLSLTLSHFLPLAGNLAWPEISPDPLILYNPGDSVFLTVAQTDADFHRLSGNQPRPA 135
           LK SLSLTLSHFLPLAGNL WP  S DP ILYNPGDSV LT+A+TDADF  L  N  R A
Sbjct: 69  LKHSLSLTLSHFLPLAGNLVWPHHSQDPFILYNPGDSVSLTIAKTDADFDHLLSNHVRKA 128

Query: 136 IESHPLLPELPATNDAALAPAMAIQLTLFPENGFSIGITTDHATHDGKSSTMFLKAWAFI 195
            ESH L+P+LP ++   +AP M++Q+TLFP++GFSIGI T H   DGK+STMFLK+WA I
Sbjct: 129 KESHFLVPQLPTSD--TIAPPMSLQITLFPKSGFSIGIITSHVVVDGKTSTMFLKSWASI 188

Query: 196 CKL--GEGNQSPSLPKELSPVFDRTAIKDYPALDRLYRGYLQRLGRSGDVKGIRFKPNNG 255
           C       N  P+LP EL+P FDRTA KD   L+RLY  +     R   +  +   P   
Sbjct: 189 CSTLNCTNNNLPTLPPELTPFFDRTAAKDPNGLERLYVKHYGAFVRQPKI--LVLTPKRV 248

Query: 256 VTPKMAKATFELTSLDVENLKKKVEKWVPGIR-LSTFVLTYAYVSSCLVKAEGLDESKKV 315
           ++  +  ATFELT +D+E L++KV       R L+TFVLTYA +S+C+VKAE ++   K+
Sbjct: 249 ISDDVVHATFELTRIDIEKLRRKVATNSTSTRHLTTFVLTYALISTCIVKAERIEPKSKI 308

Query: 316 CLATFADWRPRLDIGIAPNYLGNGGGPIGVFTGVSDFIDKNGIAIVAKKIREGITELENA 375
            L    D RPR+      NY GNG   +G F    +  ++NG+A+VA KI + I E+E +
Sbjct: 309 ALFFAVDMRPRMGALGGLNYFGNGVIGLGEFLEAKELEEENGMAMVANKISDRIMEMEKS 368

Query: 376 VTEGKMVELIMHSIGKYMKEGPVAKSVSVFGSPRFGLYEVDFGWGRPEKVEFPSVDGGNL 435
           V E K+VE++   +G++ +E P++K +   GSPRFG+YE+DFGWG  +KVE  S++    
Sbjct: 369 VKENKVVEMVEEILGRWGREMPISKMIVAGGSPRFGVYEIDFGWGNSKKVEMVSINPNGS 428

Query: 436 ISLAESRDGSGGVEVGVALPTETMQVFGSLFSEGLR 469
            S+ ESR G+GGVEVGV LP + M VF SLF E ++
Sbjct: 429 FSMTESRSGNGGVEVGVILPKDVMNVFSSLFYEEVK 458

BLAST of Moc04g36960 vs. ExPASy TrEMBL
Match: A0A6J1D383 (malonyl-CoA:anthocyanidin 5-O-glucoside-6''-O-malonyltransferase-like OS=Momordica charantia OX=3673 GN=LOC111017189 PE=3 SV=1)

HSP 1 Score: 392.1 bits (1006), Expect = 3.2e-105
Identity = 230/464 (49.57%), Postives = 290/464 (62.50%), Query Frame = 0

Query: 15  SVKILEICKITPSPS-SSSASSLSLPTTFLDTFFFQHIPNQRLLFYSLSSSDPPFLHSLL 74
           S+KILE+CKI+PSPS S +A   SLP TF D  FF   P +R+LFYS  ++D     S+L
Sbjct: 7   SLKILELCKISPSPSPSPAADQFSLPLTFFDLPFFTSPPTERILFYSHPAAD-----SIL 66

Query: 75  PQLKLSLSLTLSHFLPLAGNLAWPEISPDPLILYNPGDSVFLTVAQTD-ADFHRLSGNQP 134
            +LK SLSLTLSHFLPLAGN+ WPE SP+P ILY PGDSV LTVAQ D ADFHRLS +Q 
Sbjct: 67  QKLKHSLSLTLSHFLPLAGNVVWPEDSPEPFILYKPGDSVSLTVAQADGADFHRLSSDQI 126

Query: 135 RPAIESHPLLPELPATND-AALAPAMAIQLTLFPENGFSIGITTDHATHDGKSSTMFLKA 194
           R A ESH L+P+  A +D    AP + +Q+TLFP  GFSIGI T+H   DGK+ST FLK+
Sbjct: 127 RKANESHFLVPQFQAPDDTTCAAPILCLQITLFPGVGFSIGIATNHTVFDGKTSTTFLKS 186

Query: 195 WAFICKLGEGNQSPSLPKELSPVFDRTAIKDYPALDRLYRGYLQRLGRSGDVKG--IRFK 254
           WA IC     ++S S    L   FDR A KD   L +LY  Y Q      D  G  +R  
Sbjct: 187 WASICTHESESESESNLPTLD--FDRAAAKDPDGLQKLYLKYHQIFLSPPDTSGQRLRLA 246

Query: 255 PNNGVTPKMAKATFELTSLDVENLKKKVEKWVPGI---RLSTFVLTYAYVSSCLVKAEGL 314
           P   +   +A  TFELT  DVE+L+             RLSTFVLTYA+VS C+V+A+ L
Sbjct: 247 PKVAIPDDVAGGTFELTCADVEHLRATAAMSSSSTRRRRLSTFVLTYAHVSICIVRAQRL 306

Query: 315 DESKKVCLATFADWRPRLDIG-IAPNYLGNGGGPIGVFTGVSDFIDKNGIAIVAKKIREG 374
           D   KV L    DWRPRL    +  NY GNG G  G+F    DF  +N I +++ +I E 
Sbjct: 307 DPKTKVALFFPVDWRPRLGGRLVGENYFGNGIGGCGLFLEAKDFFGENRIGVISNQISEV 366

Query: 375 ITELENAVT-EGKMVELIMHSIGKYMKEGPVAKSVSVFGSPRFGLYEVDFGWGRPEKVEF 434
           I E+E  V    ++VEL+ H   ++  + PV K ++V GSPRFGLY++DFGWG   KVE 
Sbjct: 367 IMEMEEKVKGSTEVVELMDHVFLQWFSKMPVDKRITVAGSPRFGLYDLDFGWGGCTKVEM 426

Query: 435 PSVDGGNLISLAESRDGSGGVEVGVALPTETMQVFGSLFSEGLR 469
            S+      S+AESR+G+GG EVG+ LP + M +F SLF EGL+
Sbjct: 427 TSMGLDGTFSMAESRNGNGGAEVGIVLPQDDMNLFSSLFFEGLK 463

BLAST of Moc04g36960 vs. TAIR 10
Match: AT5G39050.1 (HXXXD-type acyl-transferase family protein )

HSP 1 Score: 305.4 bits (781), Expect = 7.6e-83
Identity = 179/473 (37.84%), Postives = 270/473 (57.08%), Query Frame = 0

Query: 15  SVKILEICKITPSPSSSSASSLSLPTTFLDTFFFQHIPNQRLLFYSLSSSDPPFLHS-LL 74
           S+K++++ ++TPS +S S+ SL+LP TF D  +++    +R++FY L+ +  PF  S ++
Sbjct: 9   SLKVIDVARVTPS-NSDSSESLTLPLTFFDLLWYKLHAVERVIFYKLTDASRPFFDSVIV 68

Query: 75  PQLKLSLSLTLSHFLPLAGNLAWPEISPDPLILYNPGDSVFLTVAQTDADFHRLSGNQPR 134
           P LK SLS +LSH+LPLAG L W  + P P I+Y P D+V  TVA+++ADF RL+G +P 
Sbjct: 69  PNLKTSLSSSLSHYLPLAGKLVWEPLDPKPKIVYTPNDAVSFTVAESNADFSRLTGKEPF 128

Query: 135 PAIESHPLLPELPATNDAALAPAMAIQLTLFPENGFSIGITTDHATHDGKSSTMFLKAWA 194
           P  E +PL+PEL  ++D+  A A++ Q+TLFP  GF I +   HA  DGK++T FLK+WA
Sbjct: 129 PTTELYPLVPELHVSDDS--ASAVSFQVTLFPNQGFCISVNAHHAVLDGKTTTNFLKSWA 188

Query: 195 FICKLGEGNQSPSLPKELSPVFDRTAIKDYPALDRLYRGYLQRLGR-------SGDVKGI 254
             CK    NQ   LP++L PV+DRT IKD   LD        R+ +         + K +
Sbjct: 189 RTCK----NQDSFLPQDLIPVYDRTVIKDPMDLDTKILNAWHRVAKVFTGGKEPENPKSL 248

Query: 255 RFKPNNGVTPKMAKATFELTSLDVENLKKKVEKWVPG---------IRLSTFVLTYAYVS 314
           +   +  + P + + T  LT  D++ L+++++K             +RLSTFV+ Y+Y  
Sbjct: 249 KLLWSPEIGPDVFRYTLNLTREDIQKLRERLKKESSSSSVSSSPKELRLSTFVIVYSYAL 308

Query: 315 SCLVKAEGLDESKKVCLATFADWRPRLDIGIAPNYLGN-GGGPIGVFTGVSDFIDKNGIA 374
           +CL+KA G D S+ V      D R  +   +  +Y GN       +      F+ + G  
Sbjct: 309 TCLIKARGGDPSRPVGYGFAVDCRSLMVPPVPSSYFGNCVSACFKMSLTAETFMSEEGFL 368

Query: 375 IVAKKIREGITELENAVTEGKMVELIMHSIGKYMKEGPVAKSVSVFGSPRFGLYEVDFGW 434
             A+ + + +  L+  V   K+ E+    +  +    P  + +SV GS RFG+Y +DFGW
Sbjct: 369 AAARMVSDSVEALDENVAL-KIPEI----LEGFTTLSPGTQVLSVAGSTRFGVYGLDFGW 428

Query: 435 GRPEKVEFPSVDGGNLISLAESRDGSGGVEVGVALPTETMQVFGSLFSEGLRS 470
           GRPEKV   S+D G  IS AESRDGSGGVE+G +L    M V   L  +GL +
Sbjct: 429 GRPEKVVVVSIDQGEAISFAESRDGSGGVELGFSLKKHEMDVLVDLLHKGLEN 469

BLAST of Moc04g36960 vs. TAIR 10
Match: AT5G39090.1 (HXXXD-type acyl-transferase family protein )

HSP 1 Score: 297.0 bits (759), Expect = 2.7e-80
Identity = 176/460 (38.26%), Postives = 269/460 (58.48%), Query Frame = 0

Query: 15  SVKILEICKITPSPSSSSASSLSLPTTFLDTFFFQHIPNQRLLFYSLSSSDPPFLHS-LL 74
           S+  + + ++TPS S+SSA SL+LP TF D  + +H   +R++FY L+  +     S ++
Sbjct: 4   SLNFIHVSRVTPSNSNSSA-SLTLPLTFFDLLWLKHKAVERVIFYKLTDVNRSLFDSVIV 63

Query: 75  PQLKLSLSLTLSHFLPLAGNLAWPEISPDPLILYNPGDSVFLTVAQTDADFHRLSGNQPR 134
           P LK SLS +LSH+LPLAG++ W    P P I+Y   D+V  TVA++++DF  L+G +P 
Sbjct: 64  PNLKSSLSSSLSHYLPLAGHIIWEPHDPKPKIVYTQNDAVSFTVAESNSDFSLLTGKEPF 123

Query: 135 PAIESHPLLPELPATNDAALAPAMAIQLTLFPENGFSIGITTDHATHDGKSSTMFLKAWA 194
            + E HPL+PEL  ++D+  A  ++ Q+TLFP  GF IG+TT HA  DGK++T FLK+WA
Sbjct: 124 SSTELHPLVPELQNSDDS--AAVVSFQVTLFPNQGFCIGVTTHHAVSDGKTTTTFLKSWA 183

Query: 195 FICKLGEGNQSPSLPKELSPVFDRTAIKDYPALDRLYRGYLQRLGRSGDVKGIRFKPNNG 254
            +CK    +Q  SLP +L P +DRT IK  P +D         + +    K ++  P   
Sbjct: 184 HLCK----HQDSSLPDDLIPFYDRTVIKGPPEIDTKVLKIWHSIHKP---KSLKLLPRPE 243

Query: 255 VTPKMAKATFELTSLDVENLKKKVEK---WVPGIRLSTFVLTYAYVSSCLVKAEGLDESK 314
           +   + + TFELT  ++E L+ K+++       +RLSTFV+T++YV +CL+ + G D ++
Sbjct: 244 IESDVVRYTFELTRENIEKLRDKLKRESSSFSSVRLSTFVITFSYVFTCLIGSGGDDPNR 303

Query: 315 KVCLATFADWRPRLDIGIAP-NYLGN-GGGPIGVFTGVSDFIDKNGIAIVAKKIREGITE 374
            V      D R  +D    P  Y GN     + +      F+ + G  + A+ I + + E
Sbjct: 304 PVGYRFAVDCRRLIDDPPIPLTYFGNCVYSAVKIPLDAGMFLGEQGFVVAARLISDSVEE 363

Query: 375 LENAVTEGKMVELIMHSIGKYMKEGPVAKSVSVFGSPRFGLYEVDFGWGRPEKVEFPSVD 434
           L++ V   K+ EL    +  Y K    ++ VSV GS RFG+Y +DFGWG+P K    S+D
Sbjct: 364 LDSNVA-WKIPEL----LETYEKAPVDSQFVSVAGSTRFGIYGLDFGWGKPFKSLLVSID 423

Query: 435 GGNLISLAESRDGSGGVEVGVALPTETMQVFGSLFSEGLR 469
               IS+AESRDGSGGVE+G +L  + M V   L  +G++
Sbjct: 424 QRGKISIAESRDGSGGVEIGFSLKKQEMNVLIDLLHKGIK 448

BLAST of Moc04g36960 vs. TAIR 10
Match: AT3G29590.1 (HXXXD-type acyl-transferase family protein )

HSP 1 Score: 296.6 bits (758), Expect = 3.6e-80
Identity = 175/457 (38.29%), Postives = 267/457 (58.42%), Query Frame = 0

Query: 15  SVKILEICKITPSPSSSSASSLSLPTTFLDTFFFQHIPNQRLLFYSLSSSDPPFLHSLLP 74
           +V ILE+ +++P     S++SL+LP T+ D  + +  P  R+LFY +         SL+ 
Sbjct: 7   AVNILEVVQVSP----PSSNSLTLPLTYFDLGWLKLHPVDRVLFYHVPELT---RSSLIS 66

Query: 75  QLKLSLSLTLSHFLPLAGNLAWPEISPDPLILYNPG--DSVFLTVAQTDADFHRLSGNQP 134
           +LK SLS TL H+LPLAG L W  I   P I+Y+P   D+V+LTVA+++ D   LSG++P
Sbjct: 67  KLKSSLSATLLHYLPLAGRLVWDSIKTKPSIVYSPDDKDAVYLTVAESNGDLSHLSGDEP 126

Query: 135 RPAIESHPLLPELPATNDAALAPAMAIQLTLFPENGFSIGITTDHATHDGKSSTMFLKAW 194
           RPA E H L+PELP ++++  A  +A+Q+T FP  GFS+G+T  HA  DGK++ MFLKAW
Sbjct: 127 RPATEFHSLVPELPVSDES--ARVLAVQVTFFPNQGFSLGVTAHHAVLDGKTTAMFLKAW 186

Query: 195 AFICKLGEGNQSPSLPKELSPVFDRTAIKDYPALDRLYRGYLQRLGRSGDVKGIRFKPNN 254
           A  CK     +  +LP +L P  DR  ++D   L+       + +  S +   ++  P+ 
Sbjct: 187 AHNCK----QEQEALPHDLVPSLDRIIVQDPTGLETKLLN--RWISASNNKPSLKLFPSK 246

Query: 255 GVTPKMAKATFELTSLDVENLKKKV--EKWVPGIRLSTFVLTYAYVSSCLVKAEGLDESK 314
            +   + + T+ LT  D++ L+++V  E     +RLSTFV+TYAYV +C+VK  G D ++
Sbjct: 247 IIGSDILRVTYRLTREDIKKLRERVETESHAKQLRLSTFVITYAYVITCMVKMRGGDPTR 306

Query: 315 KVCLATFADWRPRLDIGIAPNYLGNGGGPIGVFTGVSDFIDKNGIAIVAKKIREGITELE 374
            VC+   +D+R RL+  + P + GN     G F   ++ I + G         E +T   
Sbjct: 307 FVCVGFASDFRSRLNPPLPPTFFGNCIVGSGDFDVKAEPILEEGEGKGFITAVETLTGWV 366

Query: 375 NAVTEGKMVELIMHSIGKYMKEGPVAKSVSVFGSPRFGLYEVDFGWGRPEKVEFPSVDGG 434
           N +    + + ++     + +  P  + +SV GS R G+Y  DFGWG+P KVE  ++D  
Sbjct: 367 NGLCPENIEKNMLLPFEAFKRMEPGRQMISVAGSTRLGIYGSDFGWGKPVKVEIVTIDKD 426

Query: 435 NLISLAESRDGSGGVEVGVALPTETMQVFGSLFSEGL 468
             +SL+ES DGSGGVEVGV L  + ++ FGSLFS GL
Sbjct: 427 ASVSLSESGDGSGGVEVGVCLKKDDVERFGSLFSIGL 448

BLAST of Moc04g36960 vs. TAIR 10
Match: AT3G29670.1 (HXXXD-type acyl-transferase family protein )

HSP 1 Score: 291.2 bits (744), Expect = 1.5e-78
Identity = 170/460 (36.96%), Postives = 263/460 (57.17%), Query Frame = 0

Query: 15  SVKILEICKITPSPSS--SSASSLSLPTTFLDTFFFQHIPNQRLLFYSLSSSDPPFLHS- 74
           ++ ++E  ++TP+  S  +SA+   LP TF D  +    P +R+ FY L+ S     HS 
Sbjct: 2   TLHVIETARVTPTDYSVINSANLHKLPLTFFDLPWLLFQPVKRVFFYELTESTRDHFHSI 61

Query: 75  LLPQLKLSLSLTLSHFLPLAGNLAWPEISPDPLILYNPGDSVFLTVAQTDADFHRLSGNQ 134
           +LP+LK SLSL L ++LPL G++ W    P P I+ +    V +T+A++DADF  LSG  
Sbjct: 62  ILPKLKDSLSLILRNYLPLTGHITWEPNEPKPSIIVSENGVVLVTIAESDADFSHLSGYG 121

Query: 135 PRPAIESHPLLPELPATNDAALAPAMAIQLTLFPENGFSIGITTDHATHDGKSSTMFLKA 194
            RP  E H L+P+LP ++D+  A A +IQ+TLFP  GFSIG+   HA  DGK+S+ F+KA
Sbjct: 122 QRPLSELHALVPKLPVSDDS--ATAFSIQITLFPNQGFSIGVAAHHAVLDGKTSSTFIKA 181

Query: 195 WAFICKLGEGNQSPSLPKELSPVFDRTAIKDYPALDRLYRGYLQRLGR-SGDVKGIRFKP 254
           WA ICK     +  S+P+ L+P +DR+ IK    LD      ++ L     +++ +   P
Sbjct: 182 WAQICK----QELQSMPENLTPSYDRSLIKYPTYLDEKMIELVRSLKEDQTNIRSLTSLP 241

Query: 255 NNGVTPKMAKATFELTSLDVENLKKKVEKWVPGIRLSTFVLTYAYVSSCLVKAEGLDESK 314
           ++ +   +  AT  L+  D+E L+++V+   P + LSTFV+ YAY  +C VKA G ++ +
Sbjct: 242 SSKLGDDVVLATLVLSRADIERLREQVKNVSPSLHLSTFVIAYAYAWTCFVKARGGNKDR 301

Query: 315 KVCLATFADWRPRLDIGIAPNYLGNGGGPIGVFT-GVSDFIDKNGIAIVAKKIREGITEL 374
            V L    D+R RLD  +   Y GN   P+G +    ++F+++ G    A    E I++L
Sbjct: 302 SVSLLFVGDFRDRLDPKLPGTYFGNCMIPVGCYNRKAAEFMEEKGFVTAA----EIISDL 361

Query: 375 ENAVTEGKMVELIMHSIGKYMKEGPVAKSVSVFGSPRFGLYEVDFGWGRPEKVEFPSVDG 434
              ++  K+  +    +  +  +    +  ++ GS R G+YE DFGWGRP KV+  S+D 
Sbjct: 362 VKGLSSRKIETIADTFVEGFSFQSWSTQFGTIAGSTRLGVYEADFGWGRPVKVDIVSIDQ 421

Query: 435 GNLISLAESRDGSGGVEVGVALPTETMQVFGSLFSEGLRS 470
           G  I++AE RD SGGVE+G+ L    M    S F+ GL S
Sbjct: 422 GEAIAMAERRDESGGVEIGMCLKKTEMDSVVSFFNNGLHS 451

BLAST of Moc04g36960 vs. TAIR 10
Match: AT5G39080.1 (HXXXD-type acyl-transferase family protein )

HSP 1 Score: 288.9 bits (738), Expect = 7.4e-78
Identity = 171/461 (37.09%), Postives = 267/461 (57.92%), Query Frame = 0

Query: 15  SVKILEICKITPSPSSSSASSLSLPTTFLDTFFFQHIPNQRLLFYSLSSSDPPFLHS-LL 74
           S+ I+E+ ++TPS +S SA SLSLP T+ D  +++    +R++FY +++   PF  S ++
Sbjct: 4   SLNIIEVARVTPS-NSDSAESLSLPLTYFDLIYYKLRAVERVIFYRITNVTRPFFDSVIV 63

Query: 75  PQLKLSLSLTLSHFLPLAGNLAWPEISPDPLILYNPGDSVFLTVAQTDADFHRLSGNQPR 134
           P LK SLS  LSH+LPLAG L W  +   P I+Y+  D V  +VA+T+ADF  LSGN+P 
Sbjct: 64  PNLKTSLSSCLSHYLPLAGKLIWEPLDHKPTIVYSQNDDVSFSVAETNADFSSLSGNEPF 123

Query: 135 PAIESHPLLPELPATNDAALAPAMAIQLTLFPENGFSIGITTDHATHDGKSSTMFLKAWA 194
           P+ E +PL+P L +++D+  A  ++ Q+TLFP  GF IG++  HA  DGK++TMFLK+WA
Sbjct: 124 PSTELYPLVPALQSSDDS--ASIVSFQVTLFPNQGFCIGVSAHHAVLDGKTTTMFLKSWA 183

Query: 195 FICKLGEGNQSPSLPKELSPVFDRTAIKDYPALDRL----YRGYLQRLG---RSGDVKGI 254
            ICK    +Q  SLP++L P +DRT IK     +      +R + + L       + K +
Sbjct: 184 HICK----HQDFSLPQDLIPTYDRTVIKSPTDSENKVLNEWRSFTKILAGGKEPANPKSL 243

Query: 255 RFKPNNGVTPKMAKATFELTSLDVENLKKKVEKWVPG----------IRLSTFVLTYAYV 314
           +  P+  + P + + T +LT  D++ L++++++ V            +RLSTFV+ Y+YV
Sbjct: 244 KLNPSFEIGPDVVRYTLQLTREDIQTLRERLKREVSSSSSSTSSSKELRLSTFVIVYSYV 303

Query: 315 SSCLVKAEGLDESKKVCLATFADWRPRLDIGIAPNYLGNG-GGPIGVFTGVSDFIDKNGI 374
             C+++A G +  + V  A   D R  ++    PNY GN   G   +      F+ + G+
Sbjct: 304 LVCIIRARGGEPHRPVGYAFSVDCRSLMN-PPTPNYFGNCIAGCSRMMLTAKMFMGEEGL 363

Query: 375 AIVAKKIREGITELENAVTEGKMVELIMHSIGKYMKEGPVAKSVSVFGSPRFGLYEVDFG 434
              A  + + I E + +    K+ + +      Y    P  + + V GS RFG+YE+DFG
Sbjct: 364 LAAATMVSDSIEEWDESFA-WKIPDFV-----AYATLPPETQLILVSGSNRFGVYELDFG 423

Query: 435 WGRPEKVEFPSVDGGNLISLAESRDGSGGVEVGVALPTETM 457
           WGRP+KV   S+  GN IS+AESRD +G VE+G +L    M
Sbjct: 424 WGRPDKVMVVSISPGNGISMAESRDQNGSVEIGFSLKKHEM 450