Moc04g36350 (gene) Bitter gourd (OHB3-1) v2

Overview


Name	Moc04g36350
Type	gene
Organism	Momordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
Description	Defective in cullin neddylation protein
Location	chr4: 27298266 .. 27302046 (-)
RNA-Seq Expression	Moc04g36350
Synteny	Moc04g36350

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCTTCTGCCCCCGATTTCTTGCAAATGAATCGCTTCGACATCGTCCAGATTTATCGTCAATACTGTGGTAACTCGCTGCTCTTCCCTACTGCTTCCAATTTCCGATTCCGCCCACTTCTTTTCGATCTTGTCTCGTCCCTAATCCATGATTCGCTGCTGCCGATTCTTAGTAAACATTTCTTTAGTTTAACCTTTAGAATCTCAATTTTTCTCTGCCCTTGGATTATCTCTTGCAATCTTTTCCCCTTCAAAGGATTTTTGGTACTTTTATAGAAATCTTTCTTTGCGTCTGCCTGCCGATGTGTGTTAGAAGATTTTATTATGTTAAAGATGGAATGTTTTTATTTATCTTTTTAAAGATGGTTAGTTGCTAAGTGCTTTTTTATGCTTAACAAATTGAGATATATCTGGTCTTGAGAACTTTAGGTGCCTTTATTTTTTGGGACTTGCATTAATGTGTTCGGTGATAAAGAATGTAAAAGATTAGTTTACGGAACTTCTTTTGAAAGATATGATACATCAGGCCTCTGGGTCAATGTCAATGTTATTTTCTGGTCTATCAGCTTCCTTAGAGAATTATGGATTTTGATTATTTTATTTTCAAATGAGCAAGCACTGGTATTTCTCGTAGTTAGTCGGAGAGATAATTCAAGTATTAGTAATTATTGTATTATGATTTCAAATTCTGAGCTGCAGCCCTTTAGGGGGAGAAGTTTGAGCCTTACCAAATTGTTTCAGATAAATAGAACCTATATTCGTTTATACTTGCATGAGAAATTAATTAGTGCCCCTATGGCTTCCCATTTCTTTTAGATGACAGCAAAACGTGGGGTTGGTGGAGCTGAGTGGAAATATTCAACACTACTAAAAAAATAAAAAAGAAATGCAGTATCATGTTCTGGCTCTTAATCGTTTTTTTCTGTAAATGTAACAGTAACATGAATAAATGGTTATCATGTGCACAGGTTCCTTGACATTTTAGTTTGAATGGATTATTTCCTTGTTGTCAATGGAAAAAGATCACGGTTCTGATATTTGAACACCCCTCTTTGATTAGACTATGAATGACTTGAAATCCAGTCTGATGTGTATTGTGTTTGGAACTCAGATTAGCAATATATTGAAGTCTTAGCTGGTTTACTAACTTGTTTATAGATATTCGATCTCAAAATGGCTTTTGTTGTGGGAATGAAGACTCCAGACATGTCGAAACACAAATAGCTAAAAGTTCAAAGGAGGCACTTGCAGCGCTCTTAAAATATGTGCAATCATCACTGCAGATGGGGTACATCCCTTAAAACCTATACAATAGGCCTCGTTATGTTTTGTTAGTTACACTATGTATCACATGGACCATGATAGTATAGGATTGGATATCTAGAGTTTTACACGGTTTTATAAAACTGAATAATTTTTGTCCTTCCAAAATAAGACATTTTTTGACAGATGTGTAACTAAGATAAATTATCAACTAAATTTTCAAATTGAGATAATTTTTACTATTGTAGACACAGATACCTCCCCTACTAGTTTGATTTCATTTAGTTCTGGTACAGTCTAGTATTATTTCCTAGCATTTCTATAAAAAAAATGTTACATGGACAAAAACCGACTTGCAGGTGCCAAATTACAATTTGATTAATGAGGTCGTCAGGTCATTTTGTACGTAGGTAGCATTACATCAAATCAACCTAGGTCATCTGAAAAGTATAATTTACATTTGCAGGAACTCAATAGTTTATGAACTATCGAAGCTGATGTCATATCTAAATTTGATGGTAAACTCTTCCTTCCTATCATTTTTAGTGGTTTCCTACTCTAATTTCCTTTAATTTGGCAAGCTTTTATATTAGCTGGTGGCAACATAATATGTTCAGTAAGCTTTATCGTGTTTTATTTATGCCCTACTGTCAACTTTAGAATTATGATTGAAAGATTTTCTGGTTTGTATTTTGAATTTGACAATTTTATTTTGTAGGTAGACTTCTCTGAATTTTCACGCTTCTATGAGTTTGTTTTCTTTGTGTGCCGTGAAAATGGTCAAAAGAATATCAGTAAGATTCCTTCTTTTGACTTGGATATAAAATTATGCTTTACTTTAATATGAATGATATCATGAAATGTGCACCAGGTAAAAAAAATCTCTTACATTCTAATAAATTTATTTTTTTCTTCTTTCATTTGAATTACAGCCGTTCATAGGGCAGTTAAAGCATGGAGATTAGTTTTGGATGGGAGATTTCGGTTACTTAATCAATGGTGCAACTTTGTTGAGGTATCCTCCCCCCCATAATCAGAAAACCAGAATTTCCACCTGAAGCTATTTTCATCAACTCAGGTTTCTAGGGTCTAACATTTATGAAATGTACCTACATGTTAGACACCAATGAATGAACTTTTCTATGCTATTAGTTGTCATCCGCAGACATGCTTGTATATAAATTCATAAGTACGGACAAAGTAATATCCATTTATCTCAATTTTTGAAGTAGATGAATTTTGGGAAATTTTTGGCTATTTTTATACCAAAGATTCTAATCTATTAAATTTCTTATATCTGGGTACATGAATCCATGTTATCTTTTGGGGATCTATTCTTCAACTTGTTTCTTGATATAGAAGTACAATTTAGTGCAACTTATGTCAAGGGTTCTGATATATTTAAGTTCTATAGAACAATCAGAGACACAATATTTCTGAGGATACTTGGCAGCAAGTTTTGGCTTTCAGCCGGTGTGTACATGAAAATCTGGAAGGGTATGATCCTGAAGGTATTTGTATATATTTTTTTTTTGTTATTCTTTTAGGAGTGTACTATTAGATATTTATGTGTTAAATATATTTGAATTAAAAATGATAAGACCCTCAGGAACTACTACAATATTTTTGTAATTTTATTCGATTTTTTCTAATTAAATCGAGGCTCATGCCTCACTTCTTCTGGATAAAGAGCCTCAAGACTACATCTTAATCTTTTGAAATAGTGGGTATGATTTTTAATAAGCATAATGTTGTTTTACTGTACTGTTTAGAGTTACTCAGTTGCATTCTTCTATCAGGTGCTTGGCCTGTCCTAATTGATGACTTCGTCGAGCACATGTACAGGTTTGTGTTTGGAGGTTTATGCAGATGCGATGAGTTTTTAATTTAATGTAGTTAAATAATTCTGGCTTATACATAATTTTCAGTGGACGGTTTAGCCTTATACTAACATATGATACGCTGATAGCTTGTAACTATTATGCAGGCGTTCTAATACAATCTCAAACCTACAATGTAATTGTGGAGACACAGAATCGCAGTCATTTGAAGATTCTCTTCCTGGTATGGATTATGTTCAACTGTCAAAGAAAAAAAAAAGTAATGTTCTATAAATGGACTTCCATGGCACGCAGTATGTGATATAAGTTGTGTTTTACTGGTTTGGATTGTGTCAGGGTTAAAGTTTTTCCCTGGTTTGAAGAGGAAATTACCTGAGGACATGCAAATGGATGGGATGGTTTCTCTGGGTGATTCTAGAACTGGCTTCATGGAGCTGAGTCCTGTTACGAGCATCAAGAAAAGTAGATCTATGGCTGGCAAGCCTGTGAACTTGGAGATAAACTTACCTGCAAGTACTGCAGATGAGAGCATGGAAATGGTTCGACATAATGGTGCTGTGGCTTGTACCAAATCTCCTTGTGCTGTTGAAGGTTGTCTGTCAAAGGGGTTCGCTGGGCTTTTTTCGACGCGTTCGTTCTTGCGATTGGATCAAGAAAGGAAAGCATCATTCACGTAA

mRNA sequence

ATGGCTTCTGCCCCCGATTTCTTGCAAATGAATCGCTTCGACATCGTCCAGATTTATCGTCAATACTGTGATATTCGATCTCAAAATGGCTTTTGTTGTGGGAATGAAGACTCCAGACATGTCGAAACACAAATAGCTAAAAGTTCAAAGGAGGCACTTGCAGCGCTCTTAAAATATGTGCAATCATCACTGCAGATGGGGAACTCAATAGTTTATGAACTATCGAAGCTGATGTCATATCTAAATTTGATGGTAGACTTCTCTGAATTTTCACGCTTCTATGAGTTTGTTTTCTTTGTGTGCCGTGAAAATGGTCAAAAGAATATCACCGTTCATAGGGCAGTTAAAGCATGGAGATTAGTTTTGGATGGGAGATTTCGGTTACTTAATCAATGGTGCAACTTTGTTGAGAACAATCAGAGACACAATATTTCTGAGGATACTTGGCAGCAAGTTTTGGCTTTCAGCCGGTGTGTACATGAAAATCTGGAAGGGTATGATCCTGAAGGTGCTTGGCCTGTCCTAATTGATGACTTCGTCGAGCACATGTACAGGCGTTCTAATACAATCTCAAACCTACAATGTAATTGTGGAGACACAGAATCGCAGTCATTTGAAGATTCTCTTCCTGGGTTAAAGTTTTTCCCTGGTTTGAAGAGGAAATTACCTGAGGACATGCAAATGGATGGGATGGTTTCTCTGGGTGATTCTAGAACTGGCTTCATGGAGCTGAGTCCTGTTACGAGCATCAAGAAAAGTAGATCTATGGCTGGCAAGCCTGTGAACTTGGAGATAAACTTACCTGCAAGTACTGCAGATGAGAGCATGGAAATGGTTCGACATAATGGTGCTGTGGCTTGTACCAAATCTCCTTGTGCTGTTGAAGGTTGTCTGTCAAAGGGGTTCGCTGGGCTTTTTTCGACGCGTTCGTTCTTGCGATTGGATCAAGAAAGGAAAGCATCATTCACGTAA

Coding sequence (CDS)

ATGGCTTCTGCCCCCGATTTCTTGCAAATGAATCGCTTCGACATCGTCCAGATTTATCGTCAATACTGTGATATTCGATCTCAAAATGGCTTTTGTTGTGGGAATGAAGACTCCAGACATGTCGAAACACAAATAGCTAAAAGTTCAAAGGAGGCACTTGCAGCGCTCTTAAAATATGTGCAATCATCACTGCAGATGGGGAACTCAATAGTTTATGAACTATCGAAGCTGATGTCATATCTAAATTTGATGGTAGACTTCTCTGAATTTTCACGCTTCTATGAGTTTGTTTTCTTTGTGTGCCGTGAAAATGGTCAAAAGAATATCACCGTTCATAGGGCAGTTAAAGCATGGAGATTAGTTTTGGATGGGAGATTTCGGTTACTTAATCAATGGTGCAACTTTGTTGAGAACAATCAGAGACACAATATTTCTGAGGATACTTGGCAGCAAGTTTTGGCTTTCAGCCGGTGTGTACATGAAAATCTGGAAGGGTATGATCCTGAAGGTGCTTGGCCTGTCCTAATTGATGACTTCGTCGAGCACATGTACAGGCGTTCTAATACAATCTCAAACCTACAATGTAATTGTGGAGACACAGAATCGCAGTCATTTGAAGATTCTCTTCCTGGGTTAAAGTTTTTCCCTGGTTTGAAGAGGAAATTACCTGAGGACATGCAAATGGATGGGATGGTTTCTCTGGGTGATTCTAGAACTGGCTTCATGGAGCTGAGTCCTGTTACGAGCATCAAGAAAAGTAGATCTATGGCTGGCAAGCCTGTGAACTTGGAGATAAACTTACCTGCAAGTACTGCAGATGAGAGCATGGAAATGGTTCGACATAATGGTGCTGTGGCTTGTACCAAATCTCCTTGTGCTGTTGAAGGTTGTCTGTCAAAGGGGTTCGCTGGGCTTTTTTCGACGCGTTCGTTCTTGCGATTGGATCAAGAAAGGAAAGCATCATTCACGTAA

Protein sequence

MASAPDFLQMNRFDIVQIYRQYCDIRSQNGFCCGNEDSRHVETQIAKSSKEALAALLKYVQSSLQMGNSIVYELSKLMSYLNLMVDFSEFSRFYEFVFFVCRENGQKNITVHRAVKAWRLVLDGRFRLLNQWCNFVENNQRHNISEDTWQQVLAFSRCVHENLEGYDPEGAWPVLIDDFVEHMYRRSNTISNLQCNCGDTESQSFEDSLPGLKFFPGLKRKLPEDMQMDGMVSLGDSRTGFMELSPVTSIKKSRSMAGKPVNLEINLPASTADESMEMVRHNGAVACTKSPCAVEGCLSKGFAGLFSTRSFLRLDQERKASFT

Homology

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BLAST of Moc04g36350 vs. NCBI nr
Match: XP_022134354.1 (uncharacterized protein LOC111006635 [Momordica charantia])

HSP 1 Score: 660.2 bits (1702), Expect = 9.0e-186
Identity = 323/323 (100.00%), Postives = 323/323 (100.00%), Query Frame = 0


Query: 1   MASAPDFLQMNRFDIVQIYRQYCDIRSQNGFCCGNEDSRHVETQIAKSSKEALAALLKYV 60
           MASAPDFLQMNRFDIVQIYRQYCDIRSQNGFCCGNEDSRHVETQIAKSSKEALAALLKYV
Sbjct: 1   MASAPDFLQMNRFDIVQIYRQYCDIRSQNGFCCGNEDSRHVETQIAKSSKEALAALLKYV 60

Query: 61  QSSLQMGNSIVYELSKLMSYLNLMVDFSEFSRFYEFVFFVCRENGQKNITVHRAVKAWRL 120
           QSSLQMGNSIVYELSKLMSYLNLMVDFSEFSRFYEFVFFVCRENGQKNITVHRAVKAWRL
Sbjct: 61  QSSLQMGNSIVYELSKLMSYLNLMVDFSEFSRFYEFVFFVCRENGQKNITVHRAVKAWRL 120

Query: 121 VLDGRFRLLNQWCNFVENNQRHNISEDTWQQVLAFSRCVHENLEGYDPEGAWPVLIDDFV 180
           VLDGRFRLLNQWCNFVENNQRHNISEDTWQQVLAFSRCVHENLEGYDPEGAWPVLIDDFV
Sbjct: 121 VLDGRFRLLNQWCNFVENNQRHNISEDTWQQVLAFSRCVHENLEGYDPEGAWPVLIDDFV 180

Query: 181 EHMYRRSNTISNLQCNCGDTESQSFEDSLPGLKFFPGLKRKLPEDMQMDGMVSLGDSRTG 240
           EHMYRRSNTISNLQCNCGDTESQSFEDSLPGLKFFPGLKRKLPEDMQMDGMVSLGDSRTG
Sbjct: 181 EHMYRRSNTISNLQCNCGDTESQSFEDSLPGLKFFPGLKRKLPEDMQMDGMVSLGDSRTG 240

Query: 241 FMELSPVTSIKKSRSMAGKPVNLEINLPASTADESMEMVRHNGAVACTKSPCAVEGCLSK 300
           FMELSPVTSIKKSRSMAGKPVNLEINLPASTADESMEMVRHNGAVACTKSPCAVEGCLSK
Sbjct: 241 FMELSPVTSIKKSRSMAGKPVNLEINLPASTADESMEMVRHNGAVACTKSPCAVEGCLSK 300

Query: 301 GFAGLFSTRSFLRLDQERKASFT 324
           GFAGLFSTRSFLRLDQERKASFT
Sbjct: 301 GFAGLFSTRSFLRLDQERKASFT 323

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BLAST of Moc04g36350 vs. NCBI nr
Match: XP_038890437.1 (uncharacterized protein LOC120080000 isoform X1 [Benincasa hispida])

HSP 1 Score: 541.6 bits (1394), Expect = 4.7e-150
Identity = 274/327 (83.79%), Postives = 290/327 (88.69%), Query Frame = 0


Query: 1   MASAPDFLQMNRFDIVQIYRQYCDIRSQNGFCCGNEDSRHVETQIAKSSKEALAALLKYV 60
           M S   FL MNRFDIVQIYRQYCDIRSQNGFC GNE+S  VETQ+AKS K+ALA LL YV
Sbjct: 1   MTSLVGFLPMNRFDIVQIYRQYCDIRSQNGFCHGNEESGQVETQMAKSPKQALAELLIYV 60

Query: 61  QSSLQMGNSIVYELSKLMSYLNLMVDFSEFSRFYEFVFFVCRENGQKNITVHRAVKAWRL 120
           QSSLQMGNSIVYELSKLMSYLN MVDFSEFSRFYEFVFFVCRENGQKNITV  AV+AWRL
Sbjct: 61  QSSLQMGNSIVYELSKLMSYLNFMVDFSEFSRFYEFVFFVCRENGQKNITVSMAVRAWRL 120

Query: 121 VLDGRFRLLNQWCNFVENNQRHNISEDTWQQVLAFSRCVHENLEGYDPEGAWPVLIDDFV 180
           VL+GRFRLLNQWC+FVENNQRHNISEDTWQQVLAFSRCVHENLEGYDPEGAWPVLIDDFV
Sbjct: 121 VLNGRFRLLNQWCDFVENNQRHNISEDTWQQVLAFSRCVHENLEGYDPEGAWPVLIDDFV 180

Query: 181 EHMYRR--SNTISNLQCNCGDTESQS--FEDSLPGLKFFPGLKRKLPEDMQMDGMVSLGD 240
           EHMYR+  SN +S   CNCGD+ESQS  FEDSL GLKFFPGLKRKLPEDMQMDG++S  D
Sbjct: 181 EHMYRQSGSNKVSKFCCNCGDSESQSGVFEDSLSGLKFFPGLKRKLPEDMQMDGIMSPSD 240

Query: 241 SRTGFMELSPVTSIKKSRSMAGKPVNLEINLPASTADESMEMVRHNGAVACTKSPCAVEG 300
            RTG MELSPV S+KKSR MA +PVNLEINLP+ TADE+ EMVRHN AV  +KSPCAVEG
Sbjct: 241 PRTGSMELSPVLSVKKSRFMACRPVNLEINLPSCTADENTEMVRHNSAVG-SKSPCAVEG 300

Query: 301 CLSKGFAGLFSTRSFLRLDQERKASFT 324
           CLSKGFAGLFSTR FL LDQERKASFT
Sbjct: 301 CLSKGFAGLFSTRLFLGLDQERKASFT 326

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BLAST of Moc04g36350 vs. NCBI nr
Match: XP_004148700.1 (uncharacterized protein LOC101214663 [Cucumis sativus] >KGN52464.1 hypothetical protein Csa_007882 [Cucumis sativus])

HSP 1 Score: 524.6 bits (1350), Expect = 5.9e-145
Identity = 267/326 (81.90%), Postives = 284/326 (87.12%), Query Frame = 0


Query: 1   MASAPDFLQMNRFDIVQIYRQYCDIRSQNGFCCGNEDSRHVETQIAKSSKEALAALLKYV 60
           M S   F  MNRFDIVQIYRQYCDIRSQNG+  GNEDS + ETQ+ KSSK+ALA LL YV
Sbjct: 1   MTSLLGFSHMNRFDIVQIYRQYCDIRSQNGY-HGNEDSGNAETQMCKSSKQALAELLIYV 60

Query: 61  QSSLQMGNSIVYELSKLMSYLNLMVDFSEFSRFYEFVFFVCRENGQKNITVHRAVKAWRL 120
           QSSLQMGNS+VYELSKLMSYLN MVDFSEFSRFYEFVFFVCRENGQKNITV  AV+AWRL
Sbjct: 61  QSSLQMGNSVVYELSKLMSYLNFMVDFSEFSRFYEFVFFVCRENGQKNITVSMAVRAWRL 120

Query: 121 VLDGRFRLLNQWCNFVENNQRHNISEDTWQQVLAFSRCVHENLEGYDPEGAWPVLIDDFV 180
           VLDGRFRLLNQWC+FVENNQRHNISEDTWQQVLAFSRCVHENLEGYDPEGAWPVLIDDFV
Sbjct: 121 VLDGRFRLLNQWCDFVENNQRHNISEDTWQQVLAFSRCVHENLEGYDPEGAWPVLIDDFV 180

Query: 181 EHMYRR--SNTISNLQCNCGDT-ESQSFEDSLPGLKFFPGLKRKLPEDMQMDGMVSLGDS 240
           EHMYRR  SN +S L CNCGD+ ES  FEDSL GLKFFPGLKRKLPEDMQMDG+VS  D 
Sbjct: 181 EHMYRRSGSNKVSKLSCNCGDSEESGVFEDSLSGLKFFPGLKRKLPEDMQMDGIVSPSDP 240

Query: 241 RTGFMELSPVTSIKKSRSMAGKPVNLEINLPASTADESMEMVRHNGAVACTKSPCAVEGC 300
           RT  ME +PVTS+KKSR MA +PVNLEIN P+ TA E++EMVRHN  V  +KSPCAVEGC
Sbjct: 241 RTSAMEFTPVTSVKKSRFMACRPVNLEINSPSCTAAENIEMVRHNSTVG-SKSPCAVEGC 300

Query: 301 LSKGFAGLFSTRSFLRLDQERKASFT 324
           LSKGFAGLFSTR FL LDQERKASFT
Sbjct: 301 LSKGFAGLFSTRLFLGLDQERKASFT 324

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BLAST of Moc04g36350 vs. NCBI nr
Match: XP_008459316.1 (PREDICTED: LOW QUALITY PROTEIN: defective in cullin neddylation protein 1 [Cucumis melo])

HSP 1 Score: 524.6 bits (1350), Expect = 5.9e-145
Identity = 266/327 (81.35%), Postives = 287/327 (87.77%), Query Frame = 0


Query: 1   MASAPDFLQMNRFDIVQIYRQYCDIRSQNGFCCGNEDSRHVETQIAKSSKEALAALLKYV 60
           M S   F  MNRFDIVQIYRQYCDIRSQNG+  GNEDS + ETQ++KSSK+ALA LL YV
Sbjct: 1   MTSLLGFSHMNRFDIVQIYRQYCDIRSQNGY-HGNEDSGYAETQMSKSSKQALAELLIYV 60

Query: 61  QSSLQMGNSIVYELSKLMSYLNLMVDFSEFSRFYEFVFFVCRENGQKNITVHRAVKAWRL 120
           QSSLQMGNS+VYELSKLMSYLN MVDFSEFSRFYEF FFVCRENGQKNITV  AV+AWRL
Sbjct: 61  QSSLQMGNSVVYELSKLMSYLNFMVDFSEFSRFYEFXFFVCRENGQKNITVSMAVRAWRL 120

Query: 121 VLDGRFRLLNQWCNFVENNQRHNISEDTWQQVLAFSRCVHENLEGYDPEGAWPVLIDDFV 180
           VLDGRFRLLNQWC+FVENNQRHNISEDTWQQVLAFSRCVHENLEGYDPEGAWPVLIDDFV
Sbjct: 121 VLDGRFRLLNQWCDFVENNQRHNISEDTWQQVLAFSRCVHENLEGYDPEGAWPVLIDDFV 180

Query: 181 EHMYRR--SNTISNLQCNCGDTESQS--FEDSLPGLKFFPGLKRKLPEDMQMDGMVSLGD 240
           EHMYRR  SN +S L C CGD+ES+S  FEDSL GLKFFPGLKRKLPEDMQMDG+VS  D
Sbjct: 181 EHMYRRSGSNKVSKLCCTCGDSESESGVFEDSLSGLKFFPGLKRKLPEDMQMDGIVSPSD 240

Query: 241 SRTGFMELSPVTSIKKSRSMAGKPVNLEINLPASTADESMEMVRHNGAVACTKSPCAVEG 300
            RTG ME +PVTS+KKSR MA +PVNLEINLP+ TA E++E+VRH+  V  +KSPCAVEG
Sbjct: 241 PRTGAMEFTPVTSVKKSRFMACRPVNLEINLPSCTAAENIEVVRHSSTVG-SKSPCAVEG 300

Query: 301 CLSKGFAGLFSTRSFLRLDQERKASFT 324
           CLSKGFAGLFSTR FL LDQERKASFT
Sbjct: 301 CLSKGFAGLFSTRLFLGLDQERKASFT 325

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BLAST of Moc04g36350 vs. NCBI nr
Match: XP_023550121.1 (DCN1-like protein 1 [Cucurbita pepo subsp. pepo] >XP_023550122.1 DCN1-like protein 1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 521.5 bits (1342), Expect = 5.0e-144
Identity = 265/327 (81.04%), Postives = 285/327 (87.16%), Query Frame = 0


Query: 1   MASAPDFLQMNRFDIVQIYRQYCDIRSQNGFCCGNEDSRHVETQIAKSSKEALAALLKYV 60
           M S  DFL MNRFDIVQIYRQYCDIRSQNG   GNEDS HVE+QIAKSSK+ALA L  YV
Sbjct: 1   MTSVVDFLHMNRFDIVQIYRQYCDIRSQNGLYHGNEDSGHVESQIAKSSKQALAELFLYV 60

Query: 61  QSSLQMGNSIVYELSKLMSYLNLMVDFSEFSRFYEFVFFVCRENGQKNITVHRAVKAWRL 120
           +SSLQMGNSIV EL+K+MS+ NLMVDFSEFSRFYEFVFFV RENGQKNITV RAV+AWRL
Sbjct: 61  KSSLQMGNSIVDELAKVMSHPNLMVDFSEFSRFYEFVFFVSRENGQKNITVSRAVRAWRL 120

Query: 121 VLDGRFRLLNQWCNFVENNQRHNISEDTWQQVLAFSRCVHENLEGYDPEGAWPVLIDDFV 180
           VLDGRFRLLNQWC+FVENNQRHNISEDTW QVLAFS CVHENLEGYDPEGAWPVLIDDFV
Sbjct: 121 VLDGRFRLLNQWCDFVENNQRHNISEDTWLQVLAFSCCVHENLEGYDPEGAWPVLIDDFV 180

Query: 181 EHMYRR--SNTISNLQCNCGDTESQS--FEDSLPGLKFFPGLKRKLPEDMQMDGMVSLGD 240
           EHMYRR  SN IS L C CGD+ESQS  FEDSLPGLK FPGLKRKLPEDMQ+DG++SL D
Sbjct: 181 EHMYRRSGSNKISKLYCTCGDSESQSGVFEDSLPGLKSFPGLKRKLPEDMQVDGIMSLSD 240

Query: 241 SRTGFMELSPVTSIKKSRSMAGKPVNLEINLPASTADESMEMVRHNGAVACTKSPCAVEG 300
            RTG MEL PVTS+KKSR M  +PVNLE+NLP+ TAD + +MVR NGAV C K+PCA+EG
Sbjct: 241 PRTGSMELIPVTSVKKSRFMGCRPVNLEVNLPSCTADANTDMVRPNGAVGC-KAPCAIEG 300

Query: 301 CLSKGFAGLFSTRSFLRLDQERKASFT 324
           CLSKGFAGLFST S L LDQERKASFT
Sbjct: 301 CLSKGFAGLFSTCSVLGLDQERKASFT 326

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BLAST of Moc04g36350 vs. ExPASy Swiss-Prot
Match: Q9MBG8 (Defective in cullin neddylation protein AAR3 OS=Arabidopsis thaliana OX=3702 GN=AAR3 PE=2 SV=1)

HSP 1 Score: 273.1 bits (697), Expect = 4.1e-72
Identity = 155/314 (49.36%), Postives = 188/314 (59.87%), Query Frame = 0


Query: 12  RFDIVQIYRQYCDIRSQNGFC----CGNEDSRHVETQIAKSSKEALAALLKYVQSSLQMG 71
           RFDI +IYR++C IRS    C    C  E+S+         SKEA+  LL  V++  Q  
Sbjct: 9   RFDIFEIYRRFCAIRSGQQLCNKKPCDGEESQRFNL-----SKEAITQLLYLVENKFQAR 68

Query: 72  NSIVYELSKLMSYLNLMVDFSEFSRFYEFVFFVCRENGQKNITVHRAVKAWRLVLDGRFR 131
           NSI  EL KLMS L+LMVDF+EF+ FY+FVFF+CRENGQKNIT+ RA+ AW+LVL GRFR
Sbjct: 69  NSIFDELFKLMSRLDLMVDFTEFTCFYDFVFFMCRENGQKNITISRAITAWKLVLAGRFR 128

Query: 132 LLNQWCNFVENNQRHNISEDTWQQVLAFSRCVHENLEGYDPEGAWPVLIDDFVEHMYR-- 191
           LLN+WC+F+E NQRHNISEDTWQQVLAFSRCVHENLEGYD EGAWPVLIDDFVEHMY   
Sbjct: 129 LLNRWCDFIEKNQRHNISEDTWQQVLAFSRCVHENLEGYDSEGAWPVLIDDFVEHMYSIL 188

Query: 192 RSNTISNLQCNCGDTESQS-----------FEDSLPGLKFFPGLKRKLPEDMQMDGMVSL 251
             N  ++L C CGDTES+S           +     GL+  PGLKRK             
Sbjct: 189 GPNKDTSLFCKCGDTESESCLYQEDEHHKDYRRPHTGLRNIPGLKRK------------- 248

Query: 252 GDSRTGFMELSPVTSIKKSRSMAGKPVNLEINLPASTADESMEMVRHNGAVACTKSPCAV 309
                        TS K       +   +     +S+      +   N     +KSPC++
Sbjct: 249 -------------TSKKNDEEEEDEDEEVLETQNSSSLLNFKRIKTSNSPRCSSKSPCSI 291

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BLAST of Moc04g36350 vs. ExPASy Swiss-Prot
Match: Q9VUQ8 (DCN1-like protein OS=Drosophila melanogaster OX=7227 GN=CG7427 PE=2 SV=2)

HSP 1 Score: 103.2 bits (256), Expect = 5.6e-21
Identity = 44/96 (45.83%), Postives = 59/96 (61.46%), Query Frame = 0


Query: 86  DFSEFSRFYEFVFFVCRENGQKNITVHRAVKAWRLVLDGRFRLLNQWCNFVENNQRHNIS 145
           D  +F  FY F F   ++ GQK I +  A+  W +VL GRF+ L+ WC F+E   +  IS
Sbjct: 146 DAGKFKDFYHFTFNYAKDPGQKGIDLEMAIAYWCIVLSGRFKFLDIWCQFLEEKHKRAIS 205

Query: 146 EDTWQQVLAFSRCVHENLEGYDPEGAWPVLIDDFVE 182
            DTW  +L F+  + + +  YD EGAWPVLIDDFVE
Sbjct: 206 RDTWNLLLDFATNIDDRMSNYDSEGAWPVLIDDFVE 241

[Close]

BLAST of Moc04g36350 vs. ExPASy Swiss-Prot
Match: Q6PH85 (DCN1-like protein 2 OS=Homo sapiens OX=9606 GN=DCUN1D2 PE=1 SV=1)

HSP 1 Score: 99.8 bits (247), Expect = 6.2e-20
Identity = 46/126 (36.51%), Postives = 74/126 (58.73%), Query Frame = 0


Query: 58  KYVQSSLQMGNSIVYELSKLMSYLNL-MVDFSEFSRFYEFVFFVCRENGQKNITVHRAVK 117
           +++    ++G   + +L  L+  L   + D ++F  FY+F F   +  GQK + +  AV 
Sbjct: 121 EFLDGMTELGCDSMEKLKALLPRLEQELKDTAKFKDFYQFTFTFAKNPGQKGLDLEMAVA 180

Query: 118 AWRLVLDGRFRLLNQWCNFVENNQRHNISEDTWQQVLAFSRCVHENLEGYDPEGAWPVLI 177
            W+LVL GRF+ L+ W  F+  + + +I  DTW  +L F   + +++  YD EGAWPVLI
Sbjct: 181 YWKLVLSGRFKFLDLWNTFLMEHHKRSIPRDTWNLLLDFGNMIADDMSNYDEEGAWPVLI 240

Query: 178 DDFVEH 183
           DDFVE+
Sbjct: 241 DDFVEY 246

[Close]

BLAST of Moc04g36350 vs. ExPASy Swiss-Prot
Match: Q5ZKU1 (DCN1-like protein 1 OS=Gallus gallus OX=9031 GN=DCUN1D1 PE=2 SV=1)

HSP 1 Score: 97.4 bits (241), Expect = 3.1e-19
Identity = 41/92 (44.57%), Postives = 59/92 (64.13%), Query Frame = 0


Query: 90  FSRFYEFVFFVCRENGQKNITVHRAVKAWRLVLDGRFRLLNQWCNFVENNQRHNISEDTW 149
           F  FY+F F   +  GQK + +  A+  W LVL+GRF+ L+ W  F+  + + +I +DTW
Sbjct: 154 FKDFYQFTFNFAKNPGQKGLDLEMAIAYWNLVLNGRFKFLDLWNKFLLEHHKRSIPKDTW 213

Query: 150 QQVLAFSRCVHENLEGYDPEGAWPVLIDDFVE 182
             +L FS  + +++  YD EGAWPVLIDDFVE
Sbjct: 214 NLLLDFSTMIADDMSNYDEEGAWPVLIDDFVE 245

[Close]

BLAST of Moc04g36350 vs. ExPASy Swiss-Prot
Match: Q96GG9 (DCN1-like protein 1 OS=Homo sapiens OX=9606 GN=DCUN1D1 PE=1 SV=1)

HSP 1 Score: 97.4 bits (241), Expect = 3.1e-19
Identity = 41/92 (44.57%), Postives = 59/92 (64.13%), Query Frame = 0


Query: 90  FSRFYEFVFFVCRENGQKNITVHRAVKAWRLVLDGRFRLLNQWCNFVENNQRHNISEDTW 149
           F  FY+F F   +  GQK + +  A+  W LVL+GRF+ L+ W  F+  + + +I +DTW
Sbjct: 154 FKDFYQFTFNFAKNPGQKGLDLEMAIAYWNLVLNGRFKFLDLWNKFLLEHHKRSIPKDTW 213

Query: 150 QQVLAFSRCVHENLEGYDPEGAWPVLIDDFVE 182
             +L FS  + +++  YD EGAWPVLIDDFVE
Sbjct: 214 NLLLDFSTMIADDMSNYDEEGAWPVLIDDFVE 245

[Close]

BLAST of Moc04g36350 vs. ExPASy TrEMBL
Match: A0A6J1BYJ7 (Defective in cullin neddylation protein OS=Momordica charantia OX=3673 GN=LOC111006635 PE=4 SV=1)

HSP 1 Score: 660.2 bits (1702), Expect = 4.4e-186
Identity = 323/323 (100.00%), Postives = 323/323 (100.00%), Query Frame = 0


Query: 1   MASAPDFLQMNRFDIVQIYRQYCDIRSQNGFCCGNEDSRHVETQIAKSSKEALAALLKYV 60
           MASAPDFLQMNRFDIVQIYRQYCDIRSQNGFCCGNEDSRHVETQIAKSSKEALAALLKYV
Sbjct: 1   MASAPDFLQMNRFDIVQIYRQYCDIRSQNGFCCGNEDSRHVETQIAKSSKEALAALLKYV 60

Query: 61  QSSLQMGNSIVYELSKLMSYLNLMVDFSEFSRFYEFVFFVCRENGQKNITVHRAVKAWRL 120
           QSSLQMGNSIVYELSKLMSYLNLMVDFSEFSRFYEFVFFVCRENGQKNITVHRAVKAWRL
Sbjct: 61  QSSLQMGNSIVYELSKLMSYLNLMVDFSEFSRFYEFVFFVCRENGQKNITVHRAVKAWRL 120

Query: 121 VLDGRFRLLNQWCNFVENNQRHNISEDTWQQVLAFSRCVHENLEGYDPEGAWPVLIDDFV 180
           VLDGRFRLLNQWCNFVENNQRHNISEDTWQQVLAFSRCVHENLEGYDPEGAWPVLIDDFV
Sbjct: 121 VLDGRFRLLNQWCNFVENNQRHNISEDTWQQVLAFSRCVHENLEGYDPEGAWPVLIDDFV 180

Query: 181 EHMYRRSNTISNLQCNCGDTESQSFEDSLPGLKFFPGLKRKLPEDMQMDGMVSLGDSRTG 240
           EHMYRRSNTISNLQCNCGDTESQSFEDSLPGLKFFPGLKRKLPEDMQMDGMVSLGDSRTG
Sbjct: 181 EHMYRRSNTISNLQCNCGDTESQSFEDSLPGLKFFPGLKRKLPEDMQMDGMVSLGDSRTG 240

Query: 241 FMELSPVTSIKKSRSMAGKPVNLEINLPASTADESMEMVRHNGAVACTKSPCAVEGCLSK 300
           FMELSPVTSIKKSRSMAGKPVNLEINLPASTADESMEMVRHNGAVACTKSPCAVEGCLSK
Sbjct: 241 FMELSPVTSIKKSRSMAGKPVNLEINLPASTADESMEMVRHNGAVACTKSPCAVEGCLSK 300

Query: 301 GFAGLFSTRSFLRLDQERKASFT 324
           GFAGLFSTRSFLRLDQERKASFT
Sbjct: 301 GFAGLFSTRSFLRLDQERKASFT 323

[Close]

BLAST of Moc04g36350 vs. ExPASy TrEMBL
Match: A0A0A0KU82 (Defective in cullin neddylation protein OS=Cucumis sativus OX=3659 GN=Csa_5G636560 PE=4 SV=1)

HSP 1 Score: 524.6 bits (1350), Expect = 2.9e-145
Identity = 267/326 (81.90%), Postives = 284/326 (87.12%), Query Frame = 0


Query: 1   MASAPDFLQMNRFDIVQIYRQYCDIRSQNGFCCGNEDSRHVETQIAKSSKEALAALLKYV 60
           M S   F  MNRFDIVQIYRQYCDIRSQNG+  GNEDS + ETQ+ KSSK+ALA LL YV
Sbjct: 1   MTSLLGFSHMNRFDIVQIYRQYCDIRSQNGY-HGNEDSGNAETQMCKSSKQALAELLIYV 60

Query: 61  QSSLQMGNSIVYELSKLMSYLNLMVDFSEFSRFYEFVFFVCRENGQKNITVHRAVKAWRL 120
           QSSLQMGNS+VYELSKLMSYLN MVDFSEFSRFYEFVFFVCRENGQKNITV  AV+AWRL
Sbjct: 61  QSSLQMGNSVVYELSKLMSYLNFMVDFSEFSRFYEFVFFVCRENGQKNITVSMAVRAWRL 120

Query: 121 VLDGRFRLLNQWCNFVENNQRHNISEDTWQQVLAFSRCVHENLEGYDPEGAWPVLIDDFV 180
           VLDGRFRLLNQWC+FVENNQRHNISEDTWQQVLAFSRCVHENLEGYDPEGAWPVLIDDFV
Sbjct: 121 VLDGRFRLLNQWCDFVENNQRHNISEDTWQQVLAFSRCVHENLEGYDPEGAWPVLIDDFV 180

Query: 181 EHMYRR--SNTISNLQCNCGDT-ESQSFEDSLPGLKFFPGLKRKLPEDMQMDGMVSLGDS 240
           EHMYRR  SN +S L CNCGD+ ES  FEDSL GLKFFPGLKRKLPEDMQMDG+VS  D 
Sbjct: 181 EHMYRRSGSNKVSKLSCNCGDSEESGVFEDSLSGLKFFPGLKRKLPEDMQMDGIVSPSDP 240

Query: 241 RTGFMELSPVTSIKKSRSMAGKPVNLEINLPASTADESMEMVRHNGAVACTKSPCAVEGC 300
           RT  ME +PVTS+KKSR MA +PVNLEIN P+ TA E++EMVRHN  V  +KSPCAVEGC
Sbjct: 241 RTSAMEFTPVTSVKKSRFMACRPVNLEINSPSCTAAENIEMVRHNSTVG-SKSPCAVEGC 300

Query: 301 LSKGFAGLFSTRSFLRLDQERKASFT 324
           LSKGFAGLFSTR FL LDQERKASFT
Sbjct: 301 LSKGFAGLFSTRLFLGLDQERKASFT 324

[Close]

BLAST of Moc04g36350 vs. ExPASy TrEMBL
Match: A0A1S3CB35 (Defective in cullin neddylation protein OS=Cucumis melo OX=3656 GN=LOC103498486 PE=4 SV=1)

HSP 1 Score: 524.6 bits (1350), Expect = 2.9e-145
Identity = 266/327 (81.35%), Postives = 287/327 (87.77%), Query Frame = 0


Query: 1   MASAPDFLQMNRFDIVQIYRQYCDIRSQNGFCCGNEDSRHVETQIAKSSKEALAALLKYV 60
           M S   F  MNRFDIVQIYRQYCDIRSQNG+  GNEDS + ETQ++KSSK+ALA LL YV
Sbjct: 1   MTSLLGFSHMNRFDIVQIYRQYCDIRSQNGY-HGNEDSGYAETQMSKSSKQALAELLIYV 60

Query: 61  QSSLQMGNSIVYELSKLMSYLNLMVDFSEFSRFYEFVFFVCRENGQKNITVHRAVKAWRL 120
           QSSLQMGNS+VYELSKLMSYLN MVDFSEFSRFYEF FFVCRENGQKNITV  AV+AWRL
Sbjct: 61  QSSLQMGNSVVYELSKLMSYLNFMVDFSEFSRFYEFXFFVCRENGQKNITVSMAVRAWRL 120

Query: 121 VLDGRFRLLNQWCNFVENNQRHNISEDTWQQVLAFSRCVHENLEGYDPEGAWPVLIDDFV 180
           VLDGRFRLLNQWC+FVENNQRHNISEDTWQQVLAFSRCVHENLEGYDPEGAWPVLIDDFV
Sbjct: 121 VLDGRFRLLNQWCDFVENNQRHNISEDTWQQVLAFSRCVHENLEGYDPEGAWPVLIDDFV 180

Query: 181 EHMYRR--SNTISNLQCNCGDTESQS--FEDSLPGLKFFPGLKRKLPEDMQMDGMVSLGD 240
           EHMYRR  SN +S L C CGD+ES+S  FEDSL GLKFFPGLKRKLPEDMQMDG+VS  D
Sbjct: 181 EHMYRRSGSNKVSKLCCTCGDSESESGVFEDSLSGLKFFPGLKRKLPEDMQMDGIVSPSD 240

Query: 241 SRTGFMELSPVTSIKKSRSMAGKPVNLEINLPASTADESMEMVRHNGAVACTKSPCAVEG 300
            RTG ME +PVTS+KKSR MA +PVNLEINLP+ TA E++E+VRH+  V  +KSPCAVEG
Sbjct: 241 PRTGAMEFTPVTSVKKSRFMACRPVNLEINLPSCTAAENIEVVRHSSTVG-SKSPCAVEG 300

Query: 301 CLSKGFAGLFSTRSFLRLDQERKASFT 324
           CLSKGFAGLFSTR FL LDQERKASFT
Sbjct: 301 CLSKGFAGLFSTRLFLGLDQERKASFT 325

[Close]

BLAST of Moc04g36350 vs. ExPASy TrEMBL
Match: A0A6J1FFS5 (Defective in cullin neddylation protein OS=Cucurbita moschata OX=3662 GN=LOC111445042 PE=4 SV=1)

HSP 1 Score: 520.8 bits (1340), Expect = 4.1e-144
Identity = 264/326 (80.98%), Postives = 286/326 (87.73%), Query Frame = 0


Query: 1   MASAPDFLQMNRFDIVQIYRQYCDIRSQNGFCCGNEDSRHVETQIAKSSKEALAALLKYV 60
           M S  DFL MNRFDIVQIYRQYCDIRSQNG   GNEDS HVE+QIAKSSK+ALA L  YV
Sbjct: 1   MTSVVDFLHMNRFDIVQIYRQYCDIRSQNGLYHGNEDSGHVESQIAKSSKQALAELFLYV 60

Query: 61  QSSLQMGNSIVYELSKLMSYLNLMVDFSEFSRFYEFVFFVCRENGQKNITVHRAVKAWRL 120
           +SSLQMGNSIV EL+K+MS+ NLMVDFSEFSRFYEFVFFV RENGQKNITV RAV+AWRL
Sbjct: 61  KSSLQMGNSIVDELAKVMSHPNLMVDFSEFSRFYEFVFFVSRENGQKNITVGRAVRAWRL 120

Query: 121 VLDGRFRLLNQWCNFVENNQRHNISEDTWQQVLAFSRCVHENLEGYDPEGAWPVLIDDFV 180
           VLDGRFRLLNQWC+FVENNQRHNISEDTW QVLAFS CVHENLEGYDPEGAWPVLIDDFV
Sbjct: 121 VLDGRFRLLNQWCDFVENNQRHNISEDTWLQVLAFSCCVHENLEGYDPEGAWPVLIDDFV 180

Query: 181 EHMYRR--SNTISNLQCNCGDTESQS--FEDSLPGLKFFPGLKRKLPEDMQMDGMVSLGD 240
           EHMYRR  SN IS L C CGD+ESQS  FEDSLPGLK FPGLKRKLPEDMQ+D ++SL D
Sbjct: 181 EHMYRRSGSNKISKLYCTCGDSESQSGVFEDSLPGLKSFPGLKRKLPEDMQVDDIMSLSD 240

Query: 241 SRTGFMELSPVTSIKKSRSMAGKPVNLEINLPASTADESMEMVRHNGAVACTKSPCAVEG 300
            RTG MEL PVTS+KK+R M  +PVNLE+NLP+ TADE+++MVRHNGAV C KSPCA+EG
Sbjct: 241 PRTGSMELIPVTSLKKTRFMGCRPVNLEVNLPSCTADENIDMVRHNGAVGC-KSPCAIEG 300

Query: 301 CLSKGFAGLFSTRSFLRLDQERKASF 323
            LSKGFAGLFSTRS L LD+ERKASF
Sbjct: 301 FLSKGFAGLFSTRSILGLDRERKASF 325

[Close]

BLAST of Moc04g36350 vs. ExPASy TrEMBL
Match: A0A6J1JZZ8 (Defective in cullin neddylation protein OS=Cucurbita maxima OX=3661 GN=LOC111489407 PE=4 SV=1)

HSP 1 Score: 516.9 bits (1330), Expect = 6.0e-143
Identity = 262/327 (80.12%), Postives = 283/327 (86.54%), Query Frame = 0


Query: 1   MASAPDFLQMNRFDIVQIYRQYCDIRSQNGFCCGNEDSRHVETQIAKSSKEALAALLKYV 60
           M S  DFL MNRFDIVQIYRQYCDIRSQNG   GNED  HVE+QIAKSSK+ALA L  YV
Sbjct: 52  MTSVVDFLHMNRFDIVQIYRQYCDIRSQNGLYHGNEDCGHVESQIAKSSKQALAELFLYV 111

Query: 61  QSSLQMGNSIVYELSKLMSYLNLMVDFSEFSRFYEFVFFVCRENGQKNITVHRAVKAWRL 120
           +SSLQMGNSIV EL+K+MS+ NLMVDFSEFSRFYEFVFFV RENGQKNITV RAV+AWRL
Sbjct: 112 KSSLQMGNSIVDELAKVMSHPNLMVDFSEFSRFYEFVFFVSRENGQKNITVSRAVRAWRL 171

Query: 121 VLDGRFRLLNQWCNFVENNQRHNISEDTWQQVLAFSRCVHENLEGYDPEGAWPVLIDDFV 180
           VLDGRFRLLNQWC+FVENNQRHNISEDTW QVLAFS CVHENLEGYDPEGAWPVLIDDFV
Sbjct: 172 VLDGRFRLLNQWCDFVENNQRHNISEDTWLQVLAFSCCVHENLEGYDPEGAWPVLIDDFV 231

Query: 181 EHMYRR--SNTISNLQCNCGDTESQS--FEDSLPGLKFFPGLKRKLPEDMQMDGMVSLGD 240
           EHMYRR  SN I  L C CGD+ESQS  FEDSLPGLK FPGLKRK PEDMQ+D ++S  D
Sbjct: 232 EHMYRRSGSNKIPKLYCTCGDSESQSGVFEDSLPGLKSFPGLKRKFPEDMQVDDIMSPSD 291

Query: 241 SRTGFMELSPVTSIKKSRSMAGKPVNLEINLPASTADESMEMVRHNGAVACTKSPCAVEG 300
            RTG MEL PVTS+KKSR M  +PVNLE+NLP+ TADE+++MVR+NGAV C KSPCA+EG
Sbjct: 292 PRTGSMELIPVTSVKKSRFMGCRPVNLEVNLPSCTADENIDMVRYNGAVGC-KSPCAIEG 351

Query: 301 CLSKGFAGLFSTRSFLRLDQERKASFT 324
           CLSKGFAGLFST S L LDQERKASFT
Sbjct: 352 CLSKGFAGLFSTCSILGLDQERKASFT 377

[Close]

BLAST of Moc04g36350 vs. TAIR 10
Match: AT3G28970.1 (Domain of unknown function (DUF298) )

HSP 1 Score: 273.1 bits (697), Expect = 2.9e-73
Identity = 155/314 (49.36%), Postives = 188/314 (59.87%), Query Frame = 0


Query: 12  RFDIVQIYRQYCDIRSQNGFC----CGNEDSRHVETQIAKSSKEALAALLKYVQSSLQMG 71
           RFDI +IYR++C IRS    C    C  E+S+         SKEA+  LL  V++  Q  
Sbjct: 9   RFDIFEIYRRFCAIRSGQQLCNKKPCDGEESQRFNL-----SKEAITQLLYLVENKFQAR 68

Query: 72  NSIVYELSKLMSYLNLMVDFSEFSRFYEFVFFVCRENGQKNITVHRAVKAWRLVLDGRFR 131
           NSI  EL KLMS L+LMVDF+EF+ FY+FVFF+CRENGQKNIT+ RA+ AW+LVL GRFR
Sbjct: 69  NSIFDELFKLMSRLDLMVDFTEFTCFYDFVFFMCRENGQKNITISRAITAWKLVLAGRFR 128

Query: 132 LLNQWCNFVENNQRHNISEDTWQQVLAFSRCVHENLEGYDPEGAWPVLIDDFVEHMYR-- 191
           LLN+WC+F+E NQRHNISEDTWQQVLAFSRCVHENLEGYD EGAWPVLIDDFVEHMY   
Sbjct: 129 LLNRWCDFIEKNQRHNISEDTWQQVLAFSRCVHENLEGYDSEGAWPVLIDDFVEHMYSIL 188

Query: 192 RSNTISNLQCNCGDTESQS-----------FEDSLPGLKFFPGLKRKLPEDMQMDGMVSL 251
             N  ++L C CGDTES+S           +     GL+  PGLKRK             
Sbjct: 189 GPNKDTSLFCKCGDTESESCLYQEDEHHKDYRRPHTGLRNIPGLKRK------------- 248

Query: 252 GDSRTGFMELSPVTSIKKSRSMAGKPVNLEINLPASTADESMEMVRHNGAVACTKSPCAV 309
                        TS K       +   +     +S+      +   N     +KSPC++
Sbjct: 249 -------------TSKKNDEEEEDEDEEVLETQNSSSLLNFKRIKTSNSPRCSSKSPCSI 291

[Close]

BLAST of Moc04g36350 vs. TAIR 10
Match: AT3G12760.1 (CONTAINS InterPro DOMAIN/s: Defective-in-cullin neddylation protein (InterPro:IPR014764), Protein of unknown function DUF298 (InterPro:IPR005176), UBA-like (InterPro:IPR009060); BEST Arabidopsis thaliana protein match is: Domain of unknown function (DUF298) (TAIR:AT1G15860.2); Has 857 Blast hits to 855 proteins in 202 species: Archae - 0; Bacteria - 0; Metazoa - 482; Fungi - 154; Plants - 139; Viruses - 0; Other Eukaryotes - 82 (source: NCBI BLink). )

HSP 1 Score: 94.7 bits (234), Expect = 1.4e-19
Identity = 45/132 (34.09%), Postives = 78/132 (59.09%), Query Frame = 0


Query: 58  KYVQSSLQMGNSIVYELSKLMSYL-NLMVDFSEFSRFYEFVFFVCRENGQKNITVHRAVK 117
           +++     +G   + +L + + ++ + + D  +F   Y F F   +E GQK++ +  A+ 
Sbjct: 115 EFISGLQALGVDSIGKLQEKLPFMRSELKDEQKFHEIYNFAFGWAKEKGQKSLALDTAIG 174

Query: 118 AWRLVLDGR-FRLLNQWCNFVENNQRHNISEDTWQQVLAFSRCVHENLEGYDPEGAWPVL 177
            W+L+   R + L+  WC+F+++     IS+DTW Q+L FSR V   L  YD EGAWP L
Sbjct: 175 MWQLLFAEREWPLVTHWCDFLQDRHNKAISKDTWAQLLEFSRMVDPVLSNYDAEGAWPYL 234

Query: 178 IDDFVEHMYRRS 188
           ID+FVE++Y ++
Sbjct: 235 IDEFVEYLYDKN 246

[Close]

BLAST of Moc04g36350 vs. TAIR 10
Match: AT1G15860.1 (Domain of unknown function (DUF298) )

HSP 1 Score: 47.0 bits (110), Expect = 3.4e-05
Identity = 49/192 (25.52%), Postives = 83/192 (43.23%), Query Frame = 0


Query: 9   QMNRFDIVQIYRQYCDIRSQ----NGF--CCGNEDSRHVETQI------AKSSKEALAAL 68
           +M+R D   ++ QY +  S      G    C N +  H + +I       K+ K+     
Sbjct: 33  EMDRID--HLFNQYANKSSSLIDPEGIEELCSNLEVSHTDIRILMLAWKMKAEKQGYFTH 92

Query: 69  LKYVQSSLQMGNSIVYELSKLMSYLNLMVDF-SEFSRFYEFVFFVC-RENGQKNITVHRA 128
            ++ +    +    + +L K +  L   V   S F+ FY + F  C  E  QK+I +   
Sbjct: 93  EEWRRGLKALRADTINKLKKALPELEKEVRRPSNFADFYAYAFCYCLTEEKQKSIDIETI 152

Query: 129 VKAWRLVLDGRFRLLNQWCNFVE----NNQRHNISEDTWQQVLAF-SRCVHENLEGYDPE 182
            +   +V+   FR   Q   FVE     N    I+ D W  +  F +     ++  Y+PE
Sbjct: 153 CQLLEIVMGSTFRA--QVDYFVEYLKIQNDYKVINMDQWMGLYRFCNEISFPDMGDYNPE 212

[Close]

BLAST of Moc04g36350 vs. TAIR 10
Match: AT1G15860.3 (Domain of unknown function (DUF298) )

HSP 1 Score: 45.1 bits (105), Expect = 1.3e-04
Identity = 47/199 (23.62%), Postives = 83/199 (41.71%), Query Frame = 0


Query: 9   QMNRFDIVQIYRQYCDIRSQ----NGF--CCGNEDSRHVETQI------AKSSKEALAAL 68
           +M+R D   ++ QY +  S      G    C N +  H + +I       K+ K+     
Sbjct: 33  EMDRID--HLFNQYANKSSSLIDPEGIEELCSNLEVSHTDIRILMLAWKMKAEKQGYFTH 92

Query: 69  LKYVQSSLQMGNSIVYELSKLMSYLNLMVDF-SEFSRFYEFVFFVC-RENGQKNITVHRA 128
            ++ +    +    + +L K +  L   V   S F+ FY + F  C  E  QK+I +   
Sbjct: 93  EEWRRGLKALRADTINKLKKALPELEKEVRRPSNFADFYAYAFCYCLTEEKQKSIDIETI 152

Query: 129 VKAWRLVLDGRFR--------LLNQWC---NFVENNQRHNISEDTWQQVLAF-SRCVHEN 182
            +   +V+   FR         L  W    + +  N    I+ D W  +  F +     +
Sbjct: 153 CQLLEIVMGSTFRAQVDYFVEYLKVWITQKSHIIQNDYKVINMDQWMGLYRFCNEISFPD 212

The following BLAST results are available for this feature:

BLAST of Moc04g36350 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022134354.1	9.0e-186	100.00	uncharacterized protein LOC111006635 [Momordica charantia]	[more]
XP_038890437.1	4.7e-150	83.79	uncharacterized protein LOC120080000 isoform X1 [Benincasa hispida]	[more]
XP_004148700.1	5.9e-145	81.90	uncharacterized protein LOC101214663 [Cucumis sativus] >KGN52464.1 hypothetical ...	[more]
XP_008459316.1	5.9e-145	81.35	PREDICTED: LOW QUALITY PROTEIN: defective in cullin neddylation protein 1 [Cucum...	[more]
XP_023550121.1	5.0e-144	81.04	DCN1-like protein 1 [Cucurbita pepo subsp. pepo] >XP_023550122.1 DCN1-like prote...	[more]

BLAST of Moc04g36350 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9MBG8	4.1e-72	49.36	Defective in cullin neddylation protein AAR3 OS=Arabidopsis thaliana OX=3702 GN=...	[more]
Q9VUQ8	5.6e-21	45.83	DCN1-like protein OS=Drosophila melanogaster OX=7227 GN=CG7427 PE=2 SV=2	[more]
Q6PH85	6.2e-20	36.51	DCN1-like protein 2 OS=Homo sapiens OX=9606 GN=DCUN1D2 PE=1 SV=1	[more]
Q5ZKU1	3.1e-19	44.57	DCN1-like protein 1 OS=Gallus gallus OX=9031 GN=DCUN1D1 PE=2 SV=1	[more]
Q96GG9	3.1e-19	44.57	DCN1-like protein 1 OS=Homo sapiens OX=9606 GN=DCUN1D1 PE=1 SV=1	[more]

BLAST of Moc04g36350 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1BYJ7	4.4e-186	100.00	Defective in cullin neddylation protein OS=Momordica charantia OX=3673 GN=LOC111...	[more]
A0A0A0KU82	2.9e-145	81.90	Defective in cullin neddylation protein OS=Cucumis sativus OX=3659 GN=Csa_5G6365...	[more]
A0A1S3CB35	2.9e-145	81.35	Defective in cullin neddylation protein OS=Cucumis melo OX=3656 GN=LOC103498486 ...	[more]
A0A6J1FFS5	4.1e-144	80.98	Defective in cullin neddylation protein OS=Cucurbita moschata OX=3662 GN=LOC1114...	[more]
A0A6J1JZZ8	6.0e-143	80.12	Defective in cullin neddylation protein OS=Cucurbita maxima OX=3661 GN=LOC111489...	[more]

BLAST of Moc04g36350 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G28970.1	2.9e-73	49.36	Domain of unknown function (DUF298)	[more]
AT3G12760.1	1.4e-19	34.09	CONTAINS InterPro DOMAIN/s: Defective-in-cullin neddylation protein (InterPro:IP...	[more]
AT1G15860.1	3.4e-05	25.52	Domain of unknown function (DUF298)	[more]
AT1G15860.3	1.3e-04	23.62	Domain of unknown function (DUF298)	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (OHB3-1) v2
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR005176	Potentiating neddylation domain	PFAM	PF03556	Cullin_binding	coord: 85..182 e-value: 1.2E-29 score: 103.1
IPR005176	Potentiating neddylation domain	PROSITE	PS51229	DCUN1	coord: 1..184 score: 25.032393
IPR042460	DCN1-like, PONY binding domain	GENE3D	1.10.238.200	Cullin, PONY binding domain	coord: 86..193 e-value: 1.0E-35 score: 124.1
None	No IPR available	PANTHER	PTHR12281:SF31	DEFECTIVE IN CULLIN NEDDYLATION PROTEIN	coord: 11..232
IPR014764	Defective-in-cullin neddylation protein	PANTHER	PTHR12281	RP42 RELATED	coord: 11..232

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Moc04g36350.1	Moc04g36350.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0051443	positive regulation of ubiquitin-protein transferase activity
biological_process	GO:0045116	protein neddylation
cellular_component	GO:0000151	ubiquitin ligase complex
molecular_function	GO:0097602	cullin family protein binding
molecular_function	GO:0031624	ubiquitin conjugating enzyme binding
molecular_function	GO:0032182	ubiquitin-like protein binding