Moc04g36150 (gene) Bitter gourd (OHB3-1) v2

Overview
NameMoc04g36150
Typegene
OrganismMomordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
DescriptionPectin lyase-like superfamily protein
Locationchr4: 27124803 .. 27128161 (-)
RNA-Seq ExpressionMoc04g36150
SyntenyMoc04g36150
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAAAATGGCAAACTTTTTAGCATCCTTTGTTCTTGTGTTGGCCATTTTTGCTCAATTTCACCAAATAATTTCAGCCCAAACTTTCAATGTGATCAACTATGGAGCTGTTGGTAATGGAGCTACTGAGGACACCCAAGTAAATATGAATTTCTCTCTCTCATCCTCTCTCTCTCTCTCTCTCTCTCCATTTTGATATGAATATATATAATTTTGAATAGGCGTTTCTGAATGCTTGGAAAGCTACTTGCAAATCGAATTCATCTTCTTCTCCGACGATGGTGGTTCCCGGCGGCAAGTCATTTTTGCTTCAGCCTTTCACTTTTAATGGCCGCCGTTGCAAGTCTCCAAATATTAGAATTGTGGTTTGGATTCTTCGTTTCAATATATGAGCTTAATATTATTGTTATTATTCAATATGATTTGATATTTGTTAATTATTATATATCTTAAATACGATTAAGATACCAATTCACTCAAAAGCTTAATAGGATGGATTATGATAAATTATGTAGATATCATATCTTAACACAATAATCATAAACTCCTTCTTTATATAATTTCATTATGTCTTTTAAGTACAACGAACTAAGCTACTCCTACTGTGAATTTCATTATTATGTTTTTAAACCATATTAAAATATTTAAAAATTTAAAACTCATTGATAATCCTTTCGTGTTTTTCTCTTTAAAAAAAGTTATACCTTGATTAATCGTATATGTTTTCACCTCATCCTAATTTATAGTCAAATTTGAAAAATAAAAAAACAAAATTTTAAAACTACTTTTTTTTATTTTATATTTTATATAGTTTTCAAAACTTACCATTTTAAAACAAAAAAAAAAAACAAAACAAAAAAGCACAATAAAGAGTACTTTTAAACTTTAATTTTTAAATGGCCATATTTGGCTCAAAAAACAATTTTGCCATCACCAACCATTAACTCTTTGACAACAGGGAGAGAATTGTAAAAATATAAACTTATTTTTTCTTAAATAATGAGTCCTCAATTTAGCGGTGAAGACAAGATTTGATCCTTAACCATAAGCTAAATATTTTTCATTTTTAAATGGTGTGTTAGAACTCTATATTTAGAGAAATATTTTCAAGAATTTTTCTTAAAAAATTTATCTCAAATTCAACATGGATAATTACTTTCTCTTACTAATTTCTTTGTGTTGTACAGATCGATGGAGAGTTGATAGCACCAAAAGGTCCATCAGGATGGAAATACAAGGGAAAAAAATGTCGTCAATGGATTTTATTTGAGCACATGAAGGGTCTTAAAATCCAAGGTTCTGGTACTATCAATGGTCGTGGAAGCACTTGGTGGCGTCTCTCTTGCACACAAAATAAAAGGGTCACTTTTTCTCATCACCCTTTTTTTCCTATATAAAAGTTATATAATTTAGTTACTTTTGGTTAAATTACCACCGTAACATAGTGTGTTTGTGGTCGAATGGGATACCCTTTTCCGTCAGGATTTTCCTATTCGAGAAAATGGAGTCATCGACACCCCTTGATTGTAGAGATCTCATCCAACTGATGAAGCCACGTGGCTTCGATTTTGAGTTATAAAAAAATGTTATTTTGGTTAAATTACAACTATGGTTCTGTTCACTAACTGTGGTGTTAAATTTTTTTTTTTTTTTTTTTTAATCTTTGGCAGGTCTGTGGAAAGAAACCAACTGTAAGCAGCAATTGCCTCTTTTACATCAAATTATGTAAATTTAATATTTTTTCCAAGAAAAAGATGGAAGACACAGAGTGAAAATGTTGCATGTAAATTTTTACAGGACTGAGTTGGGTAAAAATTTTCAGGGCTTTATTTTGGAGCATTCAGAAAAAGTGCAAATAAAGGGTCTAACCTTTAAGGACAGTCCCCAAATGCATATGGCCTTTGGAAGGTCAAGAAATGTTTATGCTAACAACCTACACATTCAAGCACCTGCCAATAGTCCTAATACTGATGGTATTCATATCCAAAATTCCCAAAGAATCTTAATTCGTAGTTCTTACATTGGCACAGGTACGTAGTACATTTATTTCTCATCTTTCGAGATGACGAGCAAATAATTTTGAATTTGTATTCAACGATTATATTATGAGCCAAGTCATATTATGGTGACCTCATTGAATAAGAGTATTTTGGCAGGAGATGATTGTATTTCGATCGGGGATGGTTCGTCTAGAATTAGTATCTCCAACATTTCTTGTGGCCCGGGGCATGGCATAAGGTGATATAATTAACTTGCAAATTCAACATTAATGTCTTGTCTATCGTTACCAAATTGTTTTGTTTTGATAATTTTTTTTCCCCTTCTCTTCTCAGCATTGGAAGCCTTGGTAAACATGGACAACGAGAAACTGTTGAACATATTCATGTTAGAGATGTAACTTTTACAGGAACCACCAATGGAGCTAGAATAAAGACATGGCAGGTAATTTCTGATCTTACTCTAATCATCACATTACCAAAAAAGCTTTTTTAATACATTTGTTGGAATGAATTATGGAATACAAGTTTCACATGTTTGTTTACAAGTTTTATAAAGCGCTAGCAAAATTAACAAAATTACTTACGTATAAGTTGAGAAATTCGAACTTGCAACATTTTGGTCGAAGAGTCTTATAACTATAATAGTAAAGATATAAGAAGATGTGTGTAGGCATTATTAGATTGAAAAGTTTAACATATCAGATCTAAATTTTTAATTCATTTTGTATATGATTGTGCTTGTGATCAGGGTGGGAGAGGATATGCAAGACACATCTCCTTTGAAGGCATAACTTCTCGTGGCTCATCAAATCCAATTGTCATCGATCAATTTTACTGTGACCACGAGCAATGCAAGAACCAAGTAATAAACTAGAGTGATTTTAACCGTTTAAAACAGTAATGTTTGACAATGATATGAGATAAAGCCAAATGAAACTTTTTTTTTTCCCGCAGACTTCAGCAGTAAAGATCAGCAATGTGCTGTACAGGAAAATATCAGGGACATCAGGGAGTGAAATTGCAGTGAAACTAGCTTGCAGCGATTCAATCCCTTGTAGAAATATCATGATGAAGGACATCGAATTGATACCGACAGGTCTAGATGGAACTCAGTCGACATCGTCGTTCTGTGGGAATGTTGTGAATGGACTTCAGAATGGGACTGTCATTCCCAGTGTCCCTTGCTTGAACCAAACTAGGTAGTAGCCTAGTAGGCTTCTCCAGAGCCATGGCTCTGGTATATCTTTGTTTTTCTGTTTTGTGTTCTGTTTTGTTTGATGAAATTGAGGTTGATGAAAGTTTGGTGTACAGGCCTAGGGATAGATTAGAGTTGGAACATCAGTGTACATTCCTCGAGTAG

mRNA sequence

ATGAAAATGGCAAACTTTTTAGCATCCTTTGTTCTTGTGTTGGCCATTTTTGCTCAATTTCACCAAATAATTTCAGCCCAAACTTTCAATGTGATCAACTATGGAGCTGTTGGTAATGGAGCTACTGAGGACACCCAAGCGTTTCTGAATGCTTGGAAAGCTACTTGCAAATCGAATTCATCTTCTTCTCCGACGATGGTGGTTCCCGGCGGCAAGTCATTTTTGCTTCAGCCTTTCACTTTTAATGGCCGCCGTTGCAAGTCTCCAAATATTAGAATTGTGATCGATGGAGAGTTGATAGCACCAAAAGGTCCATCAGGATGGAAATACAAGGGAAAAAAATGTCGTCAATGGATTTTATTTGAGCACATGAAGGGTCTTAAAATCCAAGGTTCTGGTACTATCAATGGTCGTGGAAGCACTTGGTCTGTGGAAAGAAACCAACTGACTGAGTTGGGTAAAAATTTTCAGGGCTTTATTTTGGAGCATTCAGAAAAAGTGCAAATAAAGGGTCTAACCTTTAAGGACAGTCCCCAAATGCATATGGCCTTTGGAAGGTCAAGAAATGTTTATGCTAACAACCTACACATTCAAGCACCTGCCAATAGTCCTAATACTGATGGTATTCATATCCAAAATTCCCAAAGAATCTTAATTCGTAGTTCTTACATTGGCACAGGAGATGATTGTATTTCGATCGGGGATGGTTCGTCTAGAATTAGTATCTCCAACATTTCTTGTGGCCCGGGGCATGGCATAAGCATTGGAAGCCTTGGTAAACATGGACAACGAGAAACTGTTGAACATATTCATGTTAGAGATGTAACTTTTACAGGAACCACCAATGGAGCTAGAATAAAGACATGGCAGGGTGGGAGAGGATATGCAAGACACATCTCCTTTGAAGGCATAACTTCTCGTGGCTCATCAAATCCAATTGTCATCGATCAATTTTACTGTGACCACGAGCAATGCAAGAACCAAACTTCAGCAGTAAAGATCAGCAATGTGCTGTACAGGAAAATATCAGGGACATCAGGGAGTGAAATTGCAGTGAAACTAGCTTGCAGCGATTCAATCCCTTGTAGAAATATCATGATGAAGGACATCGAATTGATACCGACAGGTCTAGATGGAACTCAGTCGACATCGTCGTTCTGTGGGAATGTTGTGAATGGACTTCAGAATGGGACTGTCATTCCCAGTGTCCCTTGCTTGAACCAAACTAGGCCTAGGGATAGATTAGAGTTGGAACATCAGTGTACATTCCTCGAGTAG

Coding sequence (CDS)

ATGAAAATGGCAAACTTTTTAGCATCCTTTGTTCTTGTGTTGGCCATTTTTGCTCAATTTCACCAAATAATTTCAGCCCAAACTTTCAATGTGATCAACTATGGAGCTGTTGGTAATGGAGCTACTGAGGACACCCAAGCGTTTCTGAATGCTTGGAAAGCTACTTGCAAATCGAATTCATCTTCTTCTCCGACGATGGTGGTTCCCGGCGGCAAGTCATTTTTGCTTCAGCCTTTCACTTTTAATGGCCGCCGTTGCAAGTCTCCAAATATTAGAATTGTGATCGATGGAGAGTTGATAGCACCAAAAGGTCCATCAGGATGGAAATACAAGGGAAAAAAATGTCGTCAATGGATTTTATTTGAGCACATGAAGGGTCTTAAAATCCAAGGTTCTGGTACTATCAATGGTCGTGGAAGCACTTGGTCTGTGGAAAGAAACCAACTGACTGAGTTGGGTAAAAATTTTCAGGGCTTTATTTTGGAGCATTCAGAAAAAGTGCAAATAAAGGGTCTAACCTTTAAGGACAGTCCCCAAATGCATATGGCCTTTGGAAGGTCAAGAAATGTTTATGCTAACAACCTACACATTCAAGCACCTGCCAATAGTCCTAATACTGATGGTATTCATATCCAAAATTCCCAAAGAATCTTAATTCGTAGTTCTTACATTGGCACAGGAGATGATTGTATTTCGATCGGGGATGGTTCGTCTAGAATTAGTATCTCCAACATTTCTTGTGGCCCGGGGCATGGCATAAGCATTGGAAGCCTTGGTAAACATGGACAACGAGAAACTGTTGAACATATTCATGTTAGAGATGTAACTTTTACAGGAACCACCAATGGAGCTAGAATAAAGACATGGCAGGGTGGGAGAGGATATGCAAGACACATCTCCTTTGAAGGCATAACTTCTCGTGGCTCATCAAATCCAATTGTCATCGATCAATTTTACTGTGACCACGAGCAATGCAAGAACCAAACTTCAGCAGTAAAGATCAGCAATGTGCTGTACAGGAAAATATCAGGGACATCAGGGAGTGAAATTGCAGTGAAACTAGCTTGCAGCGATTCAATCCCTTGTAGAAATATCATGATGAAGGACATCGAATTGATACCGACAGGTCTAGATGGAACTCAGTCGACATCGTCGTTCTGTGGGAATGTTGTGAATGGACTTCAGAATGGGACTGTCATTCCCAGTGTCCCTTGCTTGAACCAAACTAGGCCTAGGGATAGATTAGAGTTGGAACATCAGTGTACATTCCTCGAGTAG

Protein sequence

MKMANFLASFVLVLAIFAQFHQIISAQTFNVINYGAVGNGATEDTQAFLNAWKATCKSNSSSSPTMVVPGGKSFLLQPFTFNGRRCKSPNIRIVIDGELIAPKGPSGWKYKGKKCRQWILFEHMKGLKIQGSGTINGRGSTWSVERNQLTELGKNFQGFILEHSEKVQIKGLTFKDSPQMHMAFGRSRNVYANNLHIQAPANSPNTDGIHIQNSQRILIRSSYIGTGDDCISIGDGSSRISISNISCGPGHGISIGSLGKHGQRETVEHIHVRDVTFTGTTNGARIKTWQGGRGYARHISFEGITSRGSSNPIVIDQFYCDHEQCKNQTSAVKISNVLYRKISGTSGSEIAVKLACSDSIPCRNIMMKDIELIPTGLDGTQSTSSFCGNVVNGLQNGTVIPSVPCLNQTRPRDRLELEHQCTFLE
Homology
BLAST of Moc04g36150 vs. NCBI nr
Match: XP_022133622.1 (probable polygalacturonase At1g80170 [Momordica charantia])

HSP 1 Score: 797.0 bits (2057), Expect = 8.1e-227
Identity = 397/411 (96.59%), Postives = 399/411 (97.08%), Query Frame = 0

Query: 3   MANFLASFVLVLAIFAQFHQIISAQTFNVINYGAVGNGATEDTQAFLNAWKATCKSNSSS 62
           MANFLASFVLVLAIFAQFHQIISAQTFNVINYGAVGNGATEDTQAFLNAWKATCKSNSSS
Sbjct: 1   MANFLASFVLVLAIFAQFHQIISAQTFNVINYGAVGNGATEDTQAFLNAWKATCKSNSSS 60

Query: 63  SPTMVVPGGKSFLLQPFTFNGRRCKSPNIRIVIDGELIAPKGPSGWKYKGKKCRQWILFE 122
           SPTMVVPGGKSFLLQPFTFNGRRCKSPNIRIVIDGELIAPKGPSGWKYKGKKCRQWILFE
Sbjct: 61  SPTMVVPGGKSFLLQPFTFNGRRCKSPNIRIVIDGELIAPKGPSGWKYKGKKCRQWILFE 120

Query: 123 HMKGLKIQGSGTINGRGSTW---SVERNQLTELGKNFQGFILEHSEKVQIKGLTFKDSPQ 182
           HMKGLKIQGSGTINGRGSTW   S  +N+    GK   GFILEHSEKVQIKGLTFKDSPQ
Sbjct: 121 HMKGLKIQGSGTINGRGSTWWRLSCTQNKRV-CGKKPTGFILEHSEKVQIKGLTFKDSPQ 180

Query: 183 MHMAFGRSRNVYANNLHIQAPANSPNTDGIHIQNSQRILIRSSYIGTGDDCISIGDGSSR 242
           MHMAFGRSRNVYANNLHIQAPANSPNTDGIHIQNSQRILIRSSYIGTGDDCISIGDGSSR
Sbjct: 181 MHMAFGRSRNVYANNLHIQAPANSPNTDGIHIQNSQRILIRSSYIGTGDDCISIGDGSSR 240

Query: 243 ISISNISCGPGHGISIGSLGKHGQRETVEHIHVRDVTFTGTTNGARIKTWQGGRGYARHI 302
           ISISNISCGPGHGISIGSLGKHGQRETVEHIHVRDVTFTGTTNGARIKTWQGGRGYARHI
Sbjct: 241 ISISNISCGPGHGISIGSLGKHGQRETVEHIHVRDVTFTGTTNGARIKTWQGGRGYARHI 300

Query: 303 SFEGITSRGSSNPIVIDQFYCDHEQCKNQTSAVKISNVLYRKISGTSGSEIAVKLACSDS 362
           SFEGITSRGSSNPIVIDQFYCDHEQCKNQTSAVKISNVLYRKISGTSGSEIAVKLACSDS
Sbjct: 301 SFEGITSRGSSNPIVIDQFYCDHEQCKNQTSAVKISNVLYRKISGTSGSEIAVKLACSDS 360

Query: 363 IPCRNIMMKDIELIPTGLDGTQSTSSFCGNVVNGLQNGTVIPSVPCLNQTR 411
           IPCRNIMMKDIELIPTGLDGTQSTSSFCGNVVNGLQNGTVIPSVPCLNQTR
Sbjct: 361 IPCRNIMMKDIELIPTGLDGTQSTSSFCGNVVNGLQNGTVIPSVPCLNQTR 410

BLAST of Moc04g36150 vs. NCBI nr
Match: XP_038889205.1 (probable polygalacturonase At1g80170 [Benincasa hispida])

HSP 1 Score: 574.3 bits (1479), Expect = 8.6e-160
Identity = 283/385 (73.51%), Postives = 320/385 (83.12%), Query Frame = 0

Query: 26  AQTFNVINYGAVGNGATEDTQAFLNAWKATCKSNSSSSPTMVVPGGKSFLLQPFTFNGRR 85
           A+TFNV+NYGA+GNG+ EDTQAFL+AWKATC S S SSP M VPGG SFLLQPFT +G  
Sbjct: 32  AKTFNVVNYGAIGNGSIEDTQAFLDAWKATCNS-SDSSPIMTVPGGNSFLLQPFTLDGSN 91

Query: 86  CKSPNIRIVIDGELIAPKGPSGWKYKGKKCRQWILFEHMKGLKIQGSGTINGRGSTW--- 145
           CKS +I IVIDGELIAPK PS WKYKGK+CRQWIL +HMKGL I+GSGTINGRG+TW   
Sbjct: 92  CKSTSISIVIDGELIAPKNPSAWKYKGKECRQWILLKHMKGLVIKGSGTINGRGNTWWHL 151

Query: 146 SVERNQLTELGKNFQGFILEHSEKVQIKGLTFKDSPQMHMAFGRSRNVYANNLHIQAPAN 205
           S +        +   G ILEHSEKV IK L FKDSPQMHMA  RS+ VY  NL+IQAP+ 
Sbjct: 152 SCKNKNKRCCRRKPTGLILEHSEKVHIKDLAFKDSPQMHMAIERSKYVYVKNLNIQAPSE 211

Query: 206 SPNTDGIHIQNSQRILIRSSYIGTGDDCISIGDGSSRISISNISCGPGHGISIGSLGKHG 265
           SPNTDGIHIQ+S++I+I  S I TGDDCISIGDGS RI+IS+I+CGPGHGISIGSLGKHG
Sbjct: 212 SPNTDGIHIQHSRKIVISMSNIRTGDDCISIGDGSYRINISSIACGPGHGISIGSLGKHG 271

Query: 266 QRETVEHIHVRDVTFTGTTNGARIKTWQGGRGYARHISFEGITSRGSSNPIVIDQFYCDH 325
             ETVE ++V+DV FTGTTNGARIKTWQGG GYAR+I+FEGI SRGSSNPI+IDQFYCDH
Sbjct: 272 GHETVEFVNVKDVIFTGTTNGARIKTWQGGSGYARNINFEGIISRGSSNPIIIDQFYCDH 331

Query: 326 EQCKNQTSAVKISNVLYRKISGTSGSEIAVKLACSDSIPCRNIMMKDIELIPTGLDGTQS 385
           E+CKNQTSAVKISNV+Y KISGTS S+IAV LACS+S+PCRNI MKDIEL     D  Q 
Sbjct: 332 EKCKNQTSAVKISNVVYSKISGTSRSKIAVNLACSESVPCRNIFMKDIELGSIHEDEQQI 391

Query: 386 TSSFCGNVVNGLQNGTVIPSVPCLN 408
           TSS+CGNVVNGL +GTVIP+VPCLN
Sbjct: 392 TSSYCGNVVNGLMDGTVIPTVPCLN 415

BLAST of Moc04g36150 vs. NCBI nr
Match: KGN52445.1 (hypothetical protein Csa_008476 [Cucumis sativus])

HSP 1 Score: 528.1 bits (1359), Expect = 7.0e-146
Identity = 271/408 (66.42%), Postives = 321/408 (78.68%), Query Frame = 0

Query: 10  FVLVLAIFAQFHQI-ISAQTFNVINYGAVGNGATEDTQAFLNAWKATCK-SNSSSSPTMV 69
           F L+L I  QF QI  S++TFNV++YGAVG+G TEDTQAFL+AWKA CK SNS +SP M+
Sbjct: 4   FFLILIISLQFLQINSSSKTFNVLDYGAVGDGNTEDTQAFLDAWKAACKSSNSKTSPIML 63

Query: 70  VPGGKSFLLQPFTFNGRRCKSPN---IRIVIDGELIAPKGPSGWKYKGKKCRQWILFEHM 129
           VP GKSFLLQPFT +G  CKS     I IVIDGELIAPK    WKY+GK+CRQWIL +HM
Sbjct: 64  VPPGKSFLLQPFTLDGYDCKSTKTNIISIVIDGELIAPKDRWAWKYEGKQCRQWILLKHM 123

Query: 130 KGLKIQGSGTINGRGST-WSVERNQLTELGKNFQGFILEHSEKVQIKGLTFKDSPQMHMA 189
           KGL ++GSGTIN +G+T W +  N+     +     ILE SEKV IK + FKDSPQMHMA
Sbjct: 124 KGLVVKGSGTINAQGNTWWDLCTNEEKVCTRRPTSLILEDSEKVHIKNIAFKDSPQMHMA 183

Query: 190 FGRSRNVYANNLHIQAPANSPNTDGIHIQNSQRILIRSSYIGTGDDCISIGDGSSRISIS 249
              S+ V+  NL+I+AP +SPNTDGIHIQ+S++I I SS I TGDDCISIGDGS RI+IS
Sbjct: 184 IENSKYVFVKNLYIEAPGDSPNTDGIHIQHSRKISITSSSIRTGDDCISIGDGSYRINIS 243

Query: 250 NISCGPGHGISIGSLGKHGQRETVEHIHVRDVTFTGTTNGARIKTWQGGRGYARHISFEG 309
            I+CGPGHGISIGSLGKHG  E VE IHV+DVTFT TTNG RIKTWQGG GYAR+I FEG
Sbjct: 244 KIACGPGHGISIGSLGKHGNHEKVEFIHVKDVTFTDTTNGVRIKTWQGGSGYARNIIFEG 303

Query: 310 ITSRGSSNPIVIDQFYCDHEQCKNQTSAVKISNVLYRKISGTSGSEIAVKLACSDSIPCR 369
           I SRGS NPI+IDQFYCDHE+CKNQTSAVK+SNVLY ++SGTS S+IAV LACS++ PCR
Sbjct: 304 IVSRGSLNPIIIDQFYCDHEKCKNQTSAVKVSNVLYNRVSGTSKSKIAVNLACSETEPCR 363

Query: 370 NIMMKDIEL--IPTGLDGTQSTSSFCGNVVNGLQNGTVIPSVPCLNQT 410
           NI+MKDI+L  I    +    TSS+C NVV+G ++G+VIP+VPCL QT
Sbjct: 364 NILMKDIKLKSIIEDEEEEDITSSYCKNVVDGHKDGSVIPNVPCLKQT 411

BLAST of Moc04g36150 vs. NCBI nr
Match: XP_031741769.1 (probable polygalacturonase At1g80170, partial [Cucumis sativus])

HSP 1 Score: 516.5 bits (1329), Expect = 2.1e-142
Identity = 260/387 (67.18%), Postives = 307/387 (79.33%), Query Frame = 0

Query: 30  NVINYGAVGNGATEDTQAFLNAWKATCK-SNSSSSPTMVVPGGKSFLLQPFTFNGRRCKS 89
           NV++YGAVG+G TEDTQAFL+AWKA CK SNS +SP M+VP GKSFLLQPFT +G  CKS
Sbjct: 1   NVLDYGAVGDGNTEDTQAFLDAWKAACKSSNSKTSPIMLVPPGKSFLLQPFTLDGYDCKS 60

Query: 90  PN---IRIVIDGELIAPKGPSGWKYKGKKCRQWILFEHMKGLKIQGSGTINGRGST-WSV 149
                I IVIDGELIAPK    WKY+GK+CRQWIL +HMKGL ++GSGTIN +G+T W +
Sbjct: 61  TKTNIISIVIDGELIAPKDRWAWKYEGKQCRQWILLKHMKGLVVKGSGTINAQGNTWWDL 120

Query: 150 ERNQLTELGKNFQGFILEHSEKVQIKGLTFKDSPQMHMAFGRSRNVYANNLHIQAPANSP 209
             N+     +     ILE SEKV IK + FKDSPQMHMA   S+ V+  NL+I+AP +SP
Sbjct: 121 CTNEEKVCTRRPTSLILEDSEKVHIKNIAFKDSPQMHMAIENSKYVFVKNLYIEAPGDSP 180

Query: 210 NTDGIHIQNSQRILIRSSYIGTGDDCISIGDGSSRISISNISCGPGHGISIGSLGKHGQR 269
           NTDGIHIQ+S++I I SS I TGDDCISIGDGS RI+IS I+CGPGHGISIGSLGKHG  
Sbjct: 181 NTDGIHIQHSRKISITSSSIRTGDDCISIGDGSYRINISKIACGPGHGISIGSLGKHGNH 240

Query: 270 ETVEHIHVRDVTFTGTTNGARIKTWQGGRGYARHISFEGITSRGSSNPIVIDQFYCDHEQ 329
           E VE IHV+DVTFT TTNG RIKTWQGG GYAR+I FEGI SRGS NPI+IDQFYCDHE+
Sbjct: 241 EKVEFIHVKDVTFTDTTNGVRIKTWQGGSGYARNIIFEGIVSRGSLNPIIIDQFYCDHEK 300

Query: 330 CKNQTSAVKISNVLYRKISGTSGSEIAVKLACSDSIPCRNIMMKDIEL--IPTGLDGTQS 389
           CKNQTSAVK+SNVLY ++SGTS S+IAV LACS++ PCRNI+MKDI+L  I    +    
Sbjct: 301 CKNQTSAVKVSNVLYNRVSGTSKSKIAVNLACSETEPCRNILMKDIKLKSIIEDEEEEDI 360

Query: 390 TSSFCGNVVNGLQNGTVIPSVPCLNQT 410
           TSS+C NVV+G ++G+VIP+VPCL QT
Sbjct: 361 TSSYCKNVVDGHKDGSVIPNVPCLKQT 387

BLAST of Moc04g36150 vs. NCBI nr
Match: XP_016902407.1 (PREDICTED: probable polygalacturonase At1g80170 [Cucumis melo])

HSP 1 Score: 515.0 bits (1325), Expect = 6.2e-142
Identity = 266/410 (64.88%), Postives = 314/410 (76.59%), Query Frame = 0

Query: 10  FVLVLAIFAQFHQI---ISAQTFNVINYGAVGNGATEDTQAFLNAWKATCK-SNSSSSPT 69
           FVL+L    +F QI    S+QTFNV++YGAVG+G TEDTQAFL+AWKA CK SNS +SP 
Sbjct: 4   FVLILITSLRFLQINSSASSQTFNVLDYGAVGDGNTEDTQAFLDAWKAACKSSNSKTSPI 63

Query: 70  MVVPGGKSFLLQPFTFNGRRCKSPN---IRIVIDGELIAPKGPSGWKYKGKKCRQWILFE 129
           M+VPGGKSFLLQPFT +G  CKS     I IVIDGELIAPK    WKY+GK+CRQWIL +
Sbjct: 64  MLVPGGKSFLLQPFTLDGHDCKSTKTNIISIVIDGELIAPKDRWAWKYEGKECRQWILLK 123

Query: 130 HMKGLKIQGSGTINGRG-STWSVERNQLTELGKNFQGFILEHSEKVQIKGLTFKDSPQMH 189
           HMKGL ++GSG+IN +G S W +  N+     +     ILE S+KV IK L FKDSPQMH
Sbjct: 124 HMKGLVVKGSGSINAQGNSWWDLCTNEEKVCTRRPTSLILEDSDKVHIKNLAFKDSPQMH 183

Query: 190 MAFGRSRNVYANNLHIQAPANSPNTDGIHIQNSQRILIRSSYIGTGDDCISIGDGSSRIS 249
           MA   S+ VY  NL+I+AP +SPNTDGIHIQ S+++ I  S I TGDDCISIGDGS RI+
Sbjct: 184 MAIESSKYVYIKNLYIEAPGDSPNTDGIHIQRSKKVSISRSSIRTGDDCISIGDGSYRIN 243

Query: 250 ISNISCGPGHGISIGSLGKHGQRETVEHIHVRDVTFTGTTNGARIKTWQGGRGYARHISF 309
           IS I+CGPGHGISIGSLGKHG  ETVE +HV+DVTFT TTNG RIKTWQGG GYAR+I F
Sbjct: 244 ISKIACGPGHGISIGSLGKHGDHETVEFVHVKDVTFTDTTNGFRIKTWQGGSGYARNIIF 303

Query: 310 EGITSRGSSNPIVIDQFYCDHEQCKNQTSAVKISNVLYRKISGTSGSEIAVKLACSDSIP 369
           EG+ SRGS NPI+IDQFYCDHE+CKN TSAVKISNV Y +ISGTS S+IAV L CS++ P
Sbjct: 304 EGMVSRGSFNPIIIDQFYCDHEKCKNHTSAVKISNVSYNRISGTSRSKIAVNLVCSETKP 363

Query: 370 CRNIMMKDIEL---IPTGLDGTQSTSSFCGNVVNGLQNGTVIPSVPCLNQ 409
           CRNI+MKDI+L   I    +    TSS C NVV+G + G+VIP+VPCL +
Sbjct: 364 CRNILMKDIKLESIIQDEEEKEDITSSLCENVVDGHKIGSVIPNVPCLKK 413

BLAST of Moc04g36150 vs. ExPASy Swiss-Prot
Match: Q94AJ5 (Probable polygalacturonase At1g80170 OS=Arabidopsis thaliana OX=3702 GN=At1g80170 PE=1 SV=1)

HSP 1 Score: 303.9 bits (777), Expect = 2.8e-81
Identity = 170/387 (43.93%), Postives = 228/387 (58.91%), Query Frame = 0

Query: 25  SAQTFNVINYGAVGNGATEDTQAFLNAWKATCKSNSSSSPTMVVPGGKSFLLQPFTFNGR 84
           S +  +V N+GA GNG T+DT+AF +AWK  C S   +   ++VP   + LL+P   +G 
Sbjct: 49  SERLLHVGNFGAKGNGVTDDTKAFADAWKTACSSKVKT--RILVPENYTCLLRPIDLSG- 108

Query: 85  RCKSPNIRIVIDGELIAPKGPSGWKYKGKKCRQWILFEHMKGLKIQGSGTINGRGSTW-- 144
            CK+  + + I G +IAP  P  W  +G   R+W+ F  +  L ++G GT+NG G  W  
Sbjct: 109 PCKA-RLTLQISGTIIAPNDPDVW--EGLNRRKWLYFHGLSRLTVEGGGTVNGMGQEWWE 168

Query: 145 -SVERNQLTELGKNFQGFILEHSEKVQIKGLTFKDSPQMHMAFGRSRNVYANNLHIQAPA 204
            S + N                 + ++++ L   DS QMH+A    R V  + L + APA
Sbjct: 169 RSCKHNHSNPCRGAPTALTFHKCKNMRVENLNVIDSQQMHIALTSCRRVTISGLKVIAPA 228

Query: 205 NSPNTDGIHIQNSQRILIRSSYIGTGDDCISIGDGSSRISISNISCGPGHGISIGSLGKH 264
            SPNTDGIHI  S+ I+I ++ + TGDDCISI   S++ISISNI CGPGHGISIGSLGK 
Sbjct: 229 TSPNTDGIHISVSRGIVIDNTTVSTGDDCISIVKNSTQISISNIICGPGHGISIGSLGKS 288

Query: 265 GQRETVEHIHVRDVTFTGTTNGARIKTWQGGRGYARHISFEGITSRGSSNPIVIDQFYCD 324
              E V  I V     + T NG RIKTWQGG G    I F  I     SNPI+IDQ+YCD
Sbjct: 289 KSWEEVRDITVDTAIISDTANGVRIKTWQGGSGLVSKIIFRNIKMNNVSNPIIIDQYYCD 348

Query: 325 -HEQCKNQTSAVKISNVLYRKISGTSGSEIAVKLACSDSIPCRNIMMKDIELIPTGLDGT 384
             + C NQTSA+ I N+ +  + GTS S+ A+K++CSDS PCRNI+++DI+L P+  DG 
Sbjct: 349 SRKPCANQTSAISIENISFVHVRGTSASKEAIKISCSDSSPCRNILLQDIDLEPSNGDG- 408

Query: 385 QSTSSFCGNVVNGLQNGTVIPSVPCLN 408
             T SFC     G  +G V P  PCL+
Sbjct: 409 -FTESFCWEAY-GSSSGQVYPP-PCLS 425

BLAST of Moc04g36150 vs. ExPASy Swiss-Prot
Match: Q9LW07 (Probable polygalacturonase At3g15720 OS=Arabidopsis thaliana OX=3702 GN=At3g15720 PE=3 SV=1)

HSP 1 Score: 300.8 bits (769), Expect = 2.4e-80
Identity = 166/385 (43.12%), Postives = 225/385 (58.44%), Query Frame = 0

Query: 25  SAQTFNVINYGAVGNGATEDTQAFLNAWKATCKSNSSSSPTMVVPGGKSFLLQPFTFNGR 84
           S+   +V  +GAVG+G T+D+QAFL AW+A C          VVP G +F+LQP  F G 
Sbjct: 20  SSNALDVTQFGAVGDGVTDDSQAFLKAWEAVCSGTGDGQ--FVVPAGMTFMLQPLKFQG- 79

Query: 85  RCKSPNIRIVIDGELIAPKGPSGWKYKGKKCRQWILFEHMKGLKIQGSGTINGRGSTWSV 144
            CKS  + + + G+L+A   PS   +KG K  QWILF  ++GL I+G G ING+GS+W  
Sbjct: 80  SCKSTPVFVQMLGKLVA---PSKGNWKGDK-DQWILFTDIEGLVIEGDGEINGQGSSWWE 139

Query: 145 ERNQLTELGKNFQGFILEHSEKVQIKGLTFKDSPQMHMAFGRSRNVYANNLHIQAPANSP 204
            +      G             +++ GLT  DSP  H+       V  ++L I AP +SP
Sbjct: 140 HK------GSRPTALKFRSCNNLRLSGLTHLDSPMAHIHISECNYVTISSLRINAPESSP 199

Query: 205 NTDGIHIQNSQRILIRSSYIGTGDDCISIGDGSSRISISNISCGPGHGISIGSLGKHGQR 264
           NTDGI +  S  ++I+   I TGDDCI+I  G+S I IS I CGPGHGISIGSLGK G+ 
Sbjct: 200 NTDGIDVGASSNVVIQDCIIATGDDCIAINSGTSNIHISGIDCGPGHGISIGSLGKDGET 259

Query: 265 ETVEHIHVRDVTFTGTTNGARIKTWQGGRGYARHISFEGITSRGSSNPIVIDQFY--CDH 324
            TVE++ V++  F GT NGARIKTWQGG GYAR I+F GIT     NPI+IDQFY   D 
Sbjct: 260 ATVENVCVQNCNFRGTMNGARIKTWQGGSGYARMITFNGITLDNVENPIIIDQFYNGGDS 319

Query: 325 EQCKN-QTSAVKISNVLYRKISGTSGSEIAVKLACSDSIPCRNIMMKDIELIPTGLDGTQ 384
           +  K+ ++SAV++S V++    GTS SE  V   CS+ +PC  I ++D+++        Q
Sbjct: 320 DNAKDRKSSAVEVSKVVFSNFIGTSKSEYGVDFRCSERVPCTEIFLRDMKIETASSGSGQ 379

Query: 385 STSSFCGNVVNGLQNGTVIPSVPCL 407
                C NV     +   +P + CL
Sbjct: 380 VAQGQCLNVRGA--STIAVPGLECL 389

BLAST of Moc04g36150 vs. ExPASy Swiss-Prot
Match: Q02096 (Polygalacturonase OS=Persea americana OX=3435 PE=2 SV=1)

HSP 1 Score: 298.9 bits (764), Expect = 9.1e-80
Identity = 165/369 (44.72%), Postives = 225/369 (60.98%), Query Frame = 0

Query: 30  NVINYGAVGNGATEDTQAFLNAWKATCKSNSSSSPTMVVPGGKSFLLQPFTFNGRRCKSP 89
           +V ++GA G+G T+DT+AF  AWK  C S S     ++VP  K++LL+  TF+G  CKS 
Sbjct: 86  SVDDFGARGDG-TDDTKAFEKAWKDACSSGS----VLIVPENKNYLLKQITFSG-PCKS- 145

Query: 90  NIRIVIDGELIAPKGPSGWKYKGKKCRQWILFEHMKGLKIQGSGTINGRGSTW---SVER 149
           ++R+ I G + A    S W   G   ++WI FE +  L ++G GT NG G TW   S +R
Sbjct: 146 DLRVKIRGTIEASSDQSDW--VGHNRKRWIEFEDISNLTLEGGGTSNGNGETWWDSSCKR 205

Query: 150 NQLTELGKNFQGFILEHSEKVQIKGLTFKDSPQMHMAFGRSRNVYANNLHIQAPANSPNT 209
            +                + + +  L+ KDS +MH++F + ++V A+NL + AP +SPNT
Sbjct: 206 KKSLPCKSAPTALTFRSCKNLIVSDLSIKDSQKMHLSFDKCQDVIASNLMVTAPEHSPNT 265

Query: 210 DGIHIQNSQRILIRSSYIGTGDDCISIGDGSSRISISNISCGPGHGISIGSLGKHGQRET 269
           DGIHI  +QRI + +S IGTGDDCISI  GS  +  +NI+CGPGHGISIGSLG       
Sbjct: 266 DGIHITGTQRIHVMNSVIGTGDDCISIESGSKMVIATNITCGPGHGISIGSLGDRNSEAH 325

Query: 270 VEHIHVRDVTFTGTTNGARIKTWQGGRGYARHISFEGITSRGSSNPIVIDQFYCD-HEQC 329
           V  + V       TTNG RIKTWQGG G A++I F+ I     +NPI+IDQ+YCD  + C
Sbjct: 326 VSGVLVDGGNLFDTTNGLRIKTWQGGSGSAKNIKFQNIVMHNVTNPIIIDQYYCDSKDPC 385

Query: 330 KNQTSAVKISNVLYRKISGTSGSEIAVKLACSDSIPCRNIMMKDIELIPTGLDGTQSTSS 389
             Q SAVK+SNV Y  I GTS SE+AVK  CS S PC+  ++ +I L+  G  G ++T S
Sbjct: 386 PEQESAVKVSNVAYMNIRGTSASEVAVKFDCSKSSPCQGYIVGNINLVGNG--GKETTMS 442

Query: 390 FCGNVVNGL 395
            C N+V GL
Sbjct: 446 -CSNIVQGL 442

BLAST of Moc04g36150 vs. ExPASy Swiss-Prot
Match: Q7M1E7 (Polygalacturonase OS=Chamaecyparis obtusa OX=13415 PE=1 SV=1)

HSP 1 Score: 293.1 bits (749), Expect = 5.0e-78
Identity = 161/405 (39.75%), Postives = 224/405 (55.31%), Query Frame = 0

Query: 25  SAQTFNVINYGAVGNGATEDTQAFLNAWKATCKSNSSSSPTMVVPGGKSFLLQPFTFNGR 84
           +A  FNV  YGAVG+G  + T+AF   W A CK    +S  ++VP  K F +    F G 
Sbjct: 55  AATVFNVEQYGAVGDGKHDSTEAFATTWNAACK---KASAVLLVPANKKFFVNNLVFRG- 114

Query: 85  RCKSPNIRIVIDGELIAPKGPSGWKYKGKKCRQWILFEHMKGLKIQGSGTINGRGSTW-- 144
            C+ P++   +DG ++A   P+ W    K  + W+ F  +    + G+G I+G+G  W  
Sbjct: 115 PCQ-PHLSFKVDGTIVAQPDPARW----KNSKIWLQFAQLTDFNLMGTGVIDGQGQQWWA 174

Query: 145 ---SVERNQLTELGKNFQGFI-LEHSEKVQIKGLTFKDSPQMHMAFGRSRNVYANNLHIQ 204
               V   +     +N    I +++S+ V +K LT  +SP+ H+ FG    V    L I+
Sbjct: 175 GQCKVVNGRTVCNDRNRPTAIKIDYSKSVTVKELTLMNSPEFHLVFGECEGVKIQGLKIK 234

Query: 205 APANSPNTDGIHIQNSQRILIRSSYIGTGDDCISIGDGSSRISISNISCGPGHGISIGSL 264
           AP +SPNTDGI I  S+R  I    IGTGDDCI+IG GSS I+I ++ CGPGHGISIGSL
Sbjct: 235 APRDSPNTDGIDIFASKRFHIEKCVIGTGDDCIAIGTGSSNITIKDLICGPGHGISIGSL 294

Query: 265 GKHGQRETVEHIHVRDVTFTGTTNGARIKTWQGGRGYARHISFEGITSRGSSNPIVIDQF 324
           G+   R  V H+HV    F  T NG RIKTWQGG G A +I++E +    S NPI+I+QF
Sbjct: 295 GRDNSRAEVSHVHVNRAKFIDTQNGLRIKTWQGGSGLASYITYENVEMINSENPILINQF 354

Query: 325 YC-DHEQCKNQTSAVKISNVLYRKISGTSGSEIAVKLACSDSIPCRNIMMKDIELIPTGL 384
           YC     C+NQ SAV+I  V Y+ I GTS +  A++L CSDS+PC  I + ++ L  T  
Sbjct: 355 YCTSASACQNQRSAVQIQGVTYKNIHGTSATAAAIQLMCSDSVPCTGIQLSNVSLKLT-- 414

Query: 385 DGTQSTSSFCGNVVNGLQNGTVIPSVPCLNQTRPRDRLELEHQCT 423
             +   +S       G  +G +IP+   L         EL+ Q T
Sbjct: 415 --SGKPASCVDKNARGFYSGRLIPTCKNLRPGPSPKEFELQQQPT 446

BLAST of Moc04g36150 vs. ExPASy Swiss-Prot
Match: Q9SFB7 (Polygalacturonase QRT2 OS=Arabidopsis thaliana OX=3702 GN=QRT2 PE=1 SV=2)

HSP 1 Score: 290.4 bits (742), Expect = 3.2e-77
Identity = 162/373 (43.43%), Postives = 231/373 (61.93%), Query Frame = 0

Query: 25  SAQTFNVINYGAVGNGATEDTQAFLNAWKATCKSNSSSSPTMVVPGGKSFLLQPFTFNGR 84
           S ++FNV  +GA  NG  +D++AF+ AW+A C  +S+    +V P  + ++L+  TF+G 
Sbjct: 67  SPRSFNVNTFGAKANG-NDDSKAFMKAWEAAC--SSTGIVYIVAPKNRDYMLKAVTFSG- 126

Query: 85  RCKSPNIRIVIDGELIAPKGPSGWKYKGKKCRQWILFEHMKGLKIQGSGTINGRGSTW-- 144
            CKS  I   I G + A + PS +K +    R WI+FE++  L+++G G I+G G  W  
Sbjct: 127 PCKSSLIIFKIYGRIEAWENPSDYKER----RHWIVFENVNNLRVEGGGRIDGNGHIWWP 186

Query: 145 -SVERN-QLTELGKNFQGFILEHSEKVQIKGLTFKDSPQMHMAFGRSRNVYANNLHIQAP 204
            S + N QL  LG       +E    +++  +  +++ QMH+ F   +NV A NL + +P
Sbjct: 187 KSCKINPQLPCLGAPTAVTFVE-CNNLRVSNIRLENAQQMHLTFQDCKNVKALNLMVTSP 246

Query: 205 ANSPNTDGIHIQNSQRILIRSSYIGTGDDCISIGDGSSRISISNISCGPGHGISIGSLGK 264
           A+SPNTDGIH+  +Q ILI+ S + TGDDCISI  GS  +  + I+CGPGHGISIGSLG+
Sbjct: 247 ADSPNTDGIHVSGTQNILIQDSIVRTGDDCISIVSGSENVRATGITCGPGHGISIGSLGE 306

Query: 265 HGQRETVEHIHVRDVTFTGTTNGARIKTWQGGRGYARHISFEGITSRGSSNPIVIDQFYC 324
                 V ++ V   T  GTTNG RIKTWQGG G A++I F+ I  +  +NPI+I+Q YC
Sbjct: 307 DNSEAYVSNVVVNKATLIGTTNGVRIKTWQGGHGMAKNIIFQDIIMKNVTNPIIINQDYC 366

Query: 325 DH-EQCKNQTSAVKISNVLYRKISGTSGSEIAVKLACSDSIPCRNIMMKDIELIPTGLDG 384
           D  E C  Q SAV++SNVLY+ I GTS   IAVK  CS +IPCR I M++++L+    D 
Sbjct: 367 DRVEACPEQKSAVQVSNVLYKNIQGTSSRPIAVKFVCSKNIPCRGISMQNVKLV----DQ 426

Query: 385 TQS--TSSFCGNV 391
           TQ   + + C NV
Sbjct: 427 TQQDVSKASCSNV 426

BLAST of Moc04g36150 vs. ExPASy TrEMBL
Match: A0A6J1BX76 (probable polygalacturonase At1g80170 OS=Momordica charantia OX=3673 GN=LOC111006160 PE=3 SV=1)

HSP 1 Score: 797.0 bits (2057), Expect = 3.9e-227
Identity = 397/411 (96.59%), Postives = 399/411 (97.08%), Query Frame = 0

Query: 3   MANFLASFVLVLAIFAQFHQIISAQTFNVINYGAVGNGATEDTQAFLNAWKATCKSNSSS 62
           MANFLASFVLVLAIFAQFHQIISAQTFNVINYGAVGNGATEDTQAFLNAWKATCKSNSSS
Sbjct: 1   MANFLASFVLVLAIFAQFHQIISAQTFNVINYGAVGNGATEDTQAFLNAWKATCKSNSSS 60

Query: 63  SPTMVVPGGKSFLLQPFTFNGRRCKSPNIRIVIDGELIAPKGPSGWKYKGKKCRQWILFE 122
           SPTMVVPGGKSFLLQPFTFNGRRCKSPNIRIVIDGELIAPKGPSGWKYKGKKCRQWILFE
Sbjct: 61  SPTMVVPGGKSFLLQPFTFNGRRCKSPNIRIVIDGELIAPKGPSGWKYKGKKCRQWILFE 120

Query: 123 HMKGLKIQGSGTINGRGSTW---SVERNQLTELGKNFQGFILEHSEKVQIKGLTFKDSPQ 182
           HMKGLKIQGSGTINGRGSTW   S  +N+    GK   GFILEHSEKVQIKGLTFKDSPQ
Sbjct: 121 HMKGLKIQGSGTINGRGSTWWRLSCTQNKRV-CGKKPTGFILEHSEKVQIKGLTFKDSPQ 180

Query: 183 MHMAFGRSRNVYANNLHIQAPANSPNTDGIHIQNSQRILIRSSYIGTGDDCISIGDGSSR 242
           MHMAFGRSRNVYANNLHIQAPANSPNTDGIHIQNSQRILIRSSYIGTGDDCISIGDGSSR
Sbjct: 181 MHMAFGRSRNVYANNLHIQAPANSPNTDGIHIQNSQRILIRSSYIGTGDDCISIGDGSSR 240

Query: 243 ISISNISCGPGHGISIGSLGKHGQRETVEHIHVRDVTFTGTTNGARIKTWQGGRGYARHI 302
           ISISNISCGPGHGISIGSLGKHGQRETVEHIHVRDVTFTGTTNGARIKTWQGGRGYARHI
Sbjct: 241 ISISNISCGPGHGISIGSLGKHGQRETVEHIHVRDVTFTGTTNGARIKTWQGGRGYARHI 300

Query: 303 SFEGITSRGSSNPIVIDQFYCDHEQCKNQTSAVKISNVLYRKISGTSGSEIAVKLACSDS 362
           SFEGITSRGSSNPIVIDQFYCDHEQCKNQTSAVKISNVLYRKISGTSGSEIAVKLACSDS
Sbjct: 301 SFEGITSRGSSNPIVIDQFYCDHEQCKNQTSAVKISNVLYRKISGTSGSEIAVKLACSDS 360

Query: 363 IPCRNIMMKDIELIPTGLDGTQSTSSFCGNVVNGLQNGTVIPSVPCLNQTR 411
           IPCRNIMMKDIELIPTGLDGTQSTSSFCGNVVNGLQNGTVIPSVPCLNQTR
Sbjct: 361 IPCRNIMMKDIELIPTGLDGTQSTSSFCGNVVNGLQNGTVIPSVPCLNQTR 410

BLAST of Moc04g36150 vs. ExPASy TrEMBL
Match: A0A0A0KS58 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G635400 PE=3 SV=1)

HSP 1 Score: 528.1 bits (1359), Expect = 3.4e-146
Identity = 271/408 (66.42%), Postives = 321/408 (78.68%), Query Frame = 0

Query: 10  FVLVLAIFAQFHQI-ISAQTFNVINYGAVGNGATEDTQAFLNAWKATCK-SNSSSSPTMV 69
           F L+L I  QF QI  S++TFNV++YGAVG+G TEDTQAFL+AWKA CK SNS +SP M+
Sbjct: 4   FFLILIISLQFLQINSSSKTFNVLDYGAVGDGNTEDTQAFLDAWKAACKSSNSKTSPIML 63

Query: 70  VPGGKSFLLQPFTFNGRRCKSPN---IRIVIDGELIAPKGPSGWKYKGKKCRQWILFEHM 129
           VP GKSFLLQPFT +G  CKS     I IVIDGELIAPK    WKY+GK+CRQWIL +HM
Sbjct: 64  VPPGKSFLLQPFTLDGYDCKSTKTNIISIVIDGELIAPKDRWAWKYEGKQCRQWILLKHM 123

Query: 130 KGLKIQGSGTINGRGST-WSVERNQLTELGKNFQGFILEHSEKVQIKGLTFKDSPQMHMA 189
           KGL ++GSGTIN +G+T W +  N+     +     ILE SEKV IK + FKDSPQMHMA
Sbjct: 124 KGLVVKGSGTINAQGNTWWDLCTNEEKVCTRRPTSLILEDSEKVHIKNIAFKDSPQMHMA 183

Query: 190 FGRSRNVYANNLHIQAPANSPNTDGIHIQNSQRILIRSSYIGTGDDCISIGDGSSRISIS 249
              S+ V+  NL+I+AP +SPNTDGIHIQ+S++I I SS I TGDDCISIGDGS RI+IS
Sbjct: 184 IENSKYVFVKNLYIEAPGDSPNTDGIHIQHSRKISITSSSIRTGDDCISIGDGSYRINIS 243

Query: 250 NISCGPGHGISIGSLGKHGQRETVEHIHVRDVTFTGTTNGARIKTWQGGRGYARHISFEG 309
            I+CGPGHGISIGSLGKHG  E VE IHV+DVTFT TTNG RIKTWQGG GYAR+I FEG
Sbjct: 244 KIACGPGHGISIGSLGKHGNHEKVEFIHVKDVTFTDTTNGVRIKTWQGGSGYARNIIFEG 303

Query: 310 ITSRGSSNPIVIDQFYCDHEQCKNQTSAVKISNVLYRKISGTSGSEIAVKLACSDSIPCR 369
           I SRGS NPI+IDQFYCDHE+CKNQTSAVK+SNVLY ++SGTS S+IAV LACS++ PCR
Sbjct: 304 IVSRGSLNPIIIDQFYCDHEKCKNQTSAVKVSNVLYNRVSGTSKSKIAVNLACSETEPCR 363

Query: 370 NIMMKDIEL--IPTGLDGTQSTSSFCGNVVNGLQNGTVIPSVPCLNQT 410
           NI+MKDI+L  I    +    TSS+C NVV+G ++G+VIP+VPCL QT
Sbjct: 364 NILMKDIKLKSIIEDEEEEDITSSYCKNVVDGHKDGSVIPNVPCLKQT 411

BLAST of Moc04g36150 vs. ExPASy TrEMBL
Match: A0A1S4E2F6 (probable polygalacturonase At1g80170 OS=Cucumis melo OX=3656 GN=LOC103498735 PE=3 SV=1)

HSP 1 Score: 515.0 bits (1325), Expect = 3.0e-142
Identity = 266/410 (64.88%), Postives = 314/410 (76.59%), Query Frame = 0

Query: 10  FVLVLAIFAQFHQI---ISAQTFNVINYGAVGNGATEDTQAFLNAWKATCK-SNSSSSPT 69
           FVL+L    +F QI    S+QTFNV++YGAVG+G TEDTQAFL+AWKA CK SNS +SP 
Sbjct: 4   FVLILITSLRFLQINSSASSQTFNVLDYGAVGDGNTEDTQAFLDAWKAACKSSNSKTSPI 63

Query: 70  MVVPGGKSFLLQPFTFNGRRCKSPN---IRIVIDGELIAPKGPSGWKYKGKKCRQWILFE 129
           M+VPGGKSFLLQPFT +G  CKS     I IVIDGELIAPK    WKY+GK+CRQWIL +
Sbjct: 64  MLVPGGKSFLLQPFTLDGHDCKSTKTNIISIVIDGELIAPKDRWAWKYEGKECRQWILLK 123

Query: 130 HMKGLKIQGSGTINGRG-STWSVERNQLTELGKNFQGFILEHSEKVQIKGLTFKDSPQMH 189
           HMKGL ++GSG+IN +G S W +  N+     +     ILE S+KV IK L FKDSPQMH
Sbjct: 124 HMKGLVVKGSGSINAQGNSWWDLCTNEEKVCTRRPTSLILEDSDKVHIKNLAFKDSPQMH 183

Query: 190 MAFGRSRNVYANNLHIQAPANSPNTDGIHIQNSQRILIRSSYIGTGDDCISIGDGSSRIS 249
           MA   S+ VY  NL+I+AP +SPNTDGIHIQ S+++ I  S I TGDDCISIGDGS RI+
Sbjct: 184 MAIESSKYVYIKNLYIEAPGDSPNTDGIHIQRSKKVSISRSSIRTGDDCISIGDGSYRIN 243

Query: 250 ISNISCGPGHGISIGSLGKHGQRETVEHIHVRDVTFTGTTNGARIKTWQGGRGYARHISF 309
           IS I+CGPGHGISIGSLGKHG  ETVE +HV+DVTFT TTNG RIKTWQGG GYAR+I F
Sbjct: 244 ISKIACGPGHGISIGSLGKHGDHETVEFVHVKDVTFTDTTNGFRIKTWQGGSGYARNIIF 303

Query: 310 EGITSRGSSNPIVIDQFYCDHEQCKNQTSAVKISNVLYRKISGTSGSEIAVKLACSDSIP 369
           EG+ SRGS NPI+IDQFYCDHE+CKN TSAVKISNV Y +ISGTS S+IAV L CS++ P
Sbjct: 304 EGMVSRGSFNPIIIDQFYCDHEKCKNHTSAVKISNVSYNRISGTSRSKIAVNLVCSETKP 363

Query: 370 CRNIMMKDIEL---IPTGLDGTQSTSSFCGNVVNGLQNGTVIPSVPCLNQ 409
           CRNI+MKDI+L   I    +    TSS C NVV+G + G+VIP+VPCL +
Sbjct: 364 CRNILMKDIKLESIIQDEEEKEDITSSLCENVVDGHKIGSVIPNVPCLKK 413

BLAST of Moc04g36150 vs. ExPASy TrEMBL
Match: A0A2N9EX52 (Uncharacterized protein OS=Fagus sylvatica OX=28930 GN=FSB_LOCUS11208 PE=3 SV=1)

HSP 1 Score: 466.1 bits (1198), Expect = 1.6e-127
Identity = 246/414 (59.42%), Postives = 302/414 (72.95%), Query Frame = 0

Query: 3   MANFLASFVLVLAIFA---QFHQII--SAQTFNVINYGAVGNGATEDTQAFLNAWKATCK 62
           MANFL   VLVLA+     Q H I+  S + +NV++YGAVG+G  +DTQAF +AW ATC+
Sbjct: 1   MANFLL-HVLVLALCVLSNQSHGILTDSVKVYNVVDYGAVGDGLADDTQAFSDAWNATCQ 60

Query: 63  SNSSSSPTMVVPGGKSFLLQPFTFNGRRCKSPNIRIVIDGELIAPKGPSGWKYKGKKCRQ 122
           S S S P M VP G +FLLQP TFNG  CKS NI + ID  +IAP  P  WK  G +C +
Sbjct: 61  S-SVSFPAMYVPQGMTFLLQPLTFNG-SCKSRNITVQIDANIIAPSDPFAWKCPGNECHR 120

Query: 123 WILFEHMKGLKIQGSGTINGRGSTW---SVERNQLTELGKNFQGFILEHSEKVQIKGLTF 182
           WI F +  GL I GSGTING+G+ W   S ++N+    GK   GF++E+S  V I GLTF
Sbjct: 121 WITFLNFNGLFISGSGTINGQGTNWWGLSCQQNK-RGCGKKPTGFVIENSNNVHIDGLTF 180

Query: 183 KDSPQMHMAFGRSRNVYANNLHIQAPANSPNTDGIHIQNSQRILIRSSYIGTGDDCISIG 242
            DSPQMH+AF RS  V+A +L IQAP +SPNTDGIHIQ+SQ I+I +  I TGDDCISI 
Sbjct: 181 VDSPQMHIAFERSTWVHATSLTIQAPKDSPNTDGIHIQHSQNIIIDNIRIATGDDCISIA 240

Query: 243 DGSSRISISNISCGPGHGISIGSLGKHGQRETVEHIHVRDVTFTGTTNGARIKTWQGGRG 302
           DGSS I+IS I CGPGHGISIGSLG  G+ ETV+ +HV+DV+FTGTTNG RIKTWQGG+G
Sbjct: 241 DGSSHINISKIDCGPGHGISIGSLGAKGKHETVQFVHVKDVSFTGTTNGVRIKTWQGGKG 300

Query: 303 YARHISFEGITSRGSSNPIVIDQFYCDHEQCKNQTSAVKISNVLYRKISGTSGSEIAVKL 362
           YARHI+FE I S GS+ PI+IDQ+YC HE C NQ SAV++S+VLY +ISGTS +EIAVK 
Sbjct: 301 YARHITFERIVSNGSARPIIIDQYYCAHEHCTNQKSAVQVSDVLYDQISGTSDAEIAVKF 360

Query: 363 ACSDSIPCRNIMMKDIELIPTGLDGTQSTSSFCGNVVNGLQNGTVIPSVPCLNQ 409
           ACS S+PC NI M++I L  T  +    TSS C N V GL+NG +IP+VPCL++
Sbjct: 361 ACSKSLPCTNIFMRNITLHST--ENGSKTSSICQN-VRGLRNGEIIPNVPCLHE 407

BLAST of Moc04g36150 vs. ExPASy TrEMBL
Match: A0A1Q3BPJ5 (Glyco_hydro_28 domain-containing protein (Fragment) OS=Cephalotus follicularis OX=3775 GN=CFOL_v3_13309 PE=3 SV=1)

HSP 1 Score: 464.9 bits (1195), Expect = 3.5e-127
Identity = 236/389 (60.67%), Postives = 289/389 (74.29%), Query Frame = 0

Query: 24  ISAQTFNVINYGAVGNGATEDTQAFLNAWKATCKSNSSSSPTMVVPGGKSFLLQPFTFNG 83
           +  + FNV++YGA+G+G T+DTQAF++AWKA C S S++S TM +P GK++LLQP  FNG
Sbjct: 4   LRTEVFNVVDYGAIGDGITDDTQAFMDAWKAACNSKSANSTTMYIPHGKTYLLQPIIFNG 63

Query: 84  RRCKSPNIRIVIDGELIAPKGPSGWKYKGKKCRQWILFEHMKG-LKIQGSGTINGRGSTW 143
            RCKS NI   IDG++ AP  P  WK    +C  WI+FEH +G L I GSGTING+G  W
Sbjct: 64  -RCKSNNINFQIDGKITAPSDPYDWKCDEDRCDHWIVFEHFEGRLSIHGSGTINGQGKNW 123

Query: 144 ---SVERNQLTELGKNFQGFILEHSEKVQIKGLTFKDSPQMHMAFGRSRNVYANNLHIQA 203
              S + +Q         GFI+ HS  V I  L F+DSPQMH+A  RSRNV+A+NL IQA
Sbjct: 124 WSLSCKHHQNI-------GFIINHSYNVHINHLRFEDSPQMHIALERSRNVHASNLTIQA 183

Query: 204 PANSPNTDGIHIQNSQRILIRSSYIGTGDDCISIGDGSSRISISNISCGPGHGISIGSLG 263
           P NSPNTDGIHIQ+S  + I ++ I TGDDCISIGDGSS ISI +I+CGPGHGISIGSLG
Sbjct: 184 PGNSPNTDGIHIQHSSNVFIHNTLIKTGDDCISIGDGSSHISIRDIACGPGHGISIGSLG 243

Query: 264 KHGQRETVEHIHVRDVTFTGTTNGARIKTWQGGRGYARHISFEGITSRGSSNPIVIDQFY 323
            +G+ ETVE + VRDV  TGTTNG RIKTWQGG+GYAR+I FE ITS GS+ PIVIDQ+Y
Sbjct: 244 VNGENETVEFVRVRDVHLTGTTNGVRIKTWQGGKGYARNILFERITSHGSTRPIVIDQYY 303

Query: 324 CDHEQCKNQTSAVKISNVLYRKISGTSGSEIAVKLACSDSIPCRNIMMKDIELIPTGLDG 383
           C H+ CKN+TSAV+ISNV+Y +ISGTS  +IAV+LACS+S PC +I MKDI L  T  DG
Sbjct: 304 CPHQYCKNETSAVQISNVMYSQISGTSHRQIAVELACSESTPCTDIFMKDIHLQSTE-DG 363

Query: 384 TQSTSSFCGNVVNGLQNGTVIPSVPCLNQ 409
            + TSS+C N V GL+N  V P+VPCL Q
Sbjct: 364 DE-TSSYCLN-VRGLRNSQVFPNVPCLLQ 381

BLAST of Moc04g36150 vs. TAIR 10
Match: AT1G56710.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 307.0 bits (785), Expect = 2.4e-83
Identity = 168/398 (42.21%), Postives = 230/398 (57.79%), Query Frame = 0

Query: 24  ISAQTFNVINYGAVGNGATEDTQAFLNAWKATCKSNSSSSPTMVVPGGKSFLLQPFTFNG 83
           I+   F+V ++GA+G+ +T+DT AF  AW A C S    S  ++VP    FL++P TFNG
Sbjct: 43  IAPTVFDVTSFGAIGDCSTDDTSAFKMAWDAACMSTGPKSALLLVPYTFCFLVKPTTFNG 102

Query: 84  RRCKSPNIRIVIDGELIAPKGPSGWKYKGKKCRQWILFEHMKGLKIQGSGTINGRGSTW- 143
             C++ N+ + IDG +++P GP  W    +  RQW++F  + GL IQGSG INGRG  W 
Sbjct: 103 -PCRT-NLVLQIDGFIVSPDGPRSWPSNYQ--RQWMMFYRVNGLSIQGSGVINGRGQKWW 162

Query: 144 ------SVERNQLTELG--KNFQGFILEHSEKVQIKGLTFKDSPQMHMAFGRSRNVYANN 203
                     N  T+ G   +     L  S KV+I+G+ F +S Q H+ F    +V  ++
Sbjct: 163 NLPCKPHKGLNGTTQTGPCDSPVAIRLFQSSKVRIQGINFMNSAQFHVRFDNCSDVVVDS 222

Query: 204 LHIQAPANSPNTDGIHIQNSQRILIRSSYIGTGDDCISIGDGSSRISISNISCGPGHGIS 263
           + I+APA+SPNTDGIHI+N+  + IR+S I  GDDCISIG G   + I N++CGP HGIS
Sbjct: 223 VIIKAPASSPNTDGIHIENTHNVQIRNSMISNGDDCISIGAGCFNVDIKNVTCGPSHGIS 282

Query: 264 IGSLGKHGQRETVEHIHVRDVTFTGTTNGARIKTWQGGRGYARHISFEGITSRGSSNPIV 323
           IGSLG H  +  V +I V + T   + NG RIKTWQGG G    I F  I      NPI+
Sbjct: 283 IGSLGVHNSQAYVSNITVTNSTIWNSDNGVRIKTWQGGSGSVSRIVFSNILMVNVRNPIM 342

Query: 324 IDQFYCDHEQCKNQTSAVKISNVLYRKISGTSG-SEIAVKLACSDSIPCRNIMMKDIELI 383
           IDQ+YC    C NQTSAV IS+VLY  I GT       +   CSDS+PC N+ + +++L 
Sbjct: 343 IDQYYCQTNNCANQTSAVIISDVLYANIKGTYDLRSPPIHFGCSDSVPCTNLTLTEVDLF 402

Query: 384 PTGLDGTQSTSSFCGNVVNGLQNGTVIPSVPCLNQTRP 412
           P+   G    + FC N    ++    +P V CL    P
Sbjct: 403 PS--KGQHLENPFCWNAYGSMK--ITVPPVYCLLDAPP 432

BLAST of Moc04g36150 vs. TAIR 10
Match: AT5G14650.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 306.2 bits (783), Expect = 4.1e-83
Identity = 167/389 (42.93%), Postives = 230/389 (59.13%), Query Frame = 0

Query: 25  SAQTFNVINYGAVGNGATEDTQAFLNAWKATCKSNSSSSPTMVVPGGKSFLLQPFTFNGR 84
           S+ T NV+++GA G+G ++DT+AF +AW+  CK  +S   T++VP G +FL+ P +F G+
Sbjct: 47  SSGTINVLDHGAKGDGTSDDTKAFEDAWQVACKVAAS---TLLVPSGSTFLVGPVSFLGK 106

Query: 85  RCKSPNIRIVIDGELIAPKGPSGWKYKGKKCRQWILFEHMKGLKIQGSGTINGRGSTW-- 144
            CK   I   ++G++IAP   S W   G    QWI F+ ++G+ I+G G I+GRGS W  
Sbjct: 107 ECKE-KIVFQLEGKIIAPTSASAW---GSGLLQWIEFKALQGITIKGKGIIDGRGSVWWN 166

Query: 145 -----SVERNQLTELGKNFQGFILEHSEKVQIKGLTFKDSPQMHMAFGRSRNVYANNLHI 204
                 + R + T L   F G     S  V + G+T ++SPQ H+ F    ++  ++   
Sbjct: 167 DMMGTKMPRTKPTAL--RFYG-----SNGVTVSGITIQNSPQTHLKFDNCISIQVSDFTT 226

Query: 205 QAPANSPNTDGIHIQNSQRILIRSSYIGTGDDCISIGDGSSRISISNISCGPGHGISIGS 264
            +P +SPNTDGIH+QNSQ  +I  S +  GDDCISI  G S I+I ++ CGPGHGISIG 
Sbjct: 227 SSPGDSPNTDGIHLQNSQDAVIYRSTLACGDDCISIQTGCSNINIHDVDCGPGHGISIGG 286

Query: 265 LGKHGQRETVEHIHVRDVTFTGTTNGARIKTWQGGRGYARHISFEGITSRGSSNPIVIDQ 324
           LGK   +  V +I VRDVT   TTNG RIK+WQGG G  + + F  I     +NPI+IDQ
Sbjct: 287 LGKDNTKACVSNITVRDVTMHETTNGVRIKSWQGGSGSVKQVMFSNIQVSNVANPIIIDQ 346

Query: 325 FYCDHEQCKNQTSAVKISNVLYRKISGTSGSEIAVKLACSDSIPCRNIMMKDIELIPTGL 384
           +YCD   C N+TSAV +SN+ Y  I GT   E  V+ ACSDS+PC  I +  IEL P   
Sbjct: 347 YYCDGGGCHNETSAVAVSNINYINIKGTYTKE-PVRFACSDSLPCTGISLSTIELKP-AT 406

Query: 385 DGTQSTSSFCGNVVNGLQNGTVIPSVPCL 407
               S   FC      L+  T +P + CL
Sbjct: 407 GKASSLDPFCWKAHGELKTKT-LPPIQCL 418

BLAST of Moc04g36150 vs. TAIR 10
Match: AT1G80170.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 303.9 bits (777), Expect = 2.0e-82
Identity = 170/387 (43.93%), Postives = 228/387 (58.91%), Query Frame = 0

Query: 25  SAQTFNVINYGAVGNGATEDTQAFLNAWKATCKSNSSSSPTMVVPGGKSFLLQPFTFNGR 84
           S +  +V N+GA GNG T+DT+AF +AWK  C S   +   ++VP   + LL+P   +G 
Sbjct: 49  SERLLHVGNFGAKGNGVTDDTKAFADAWKTACSSKVKT--RILVPENYTCLLRPIDLSG- 108

Query: 85  RCKSPNIRIVIDGELIAPKGPSGWKYKGKKCRQWILFEHMKGLKIQGSGTINGRGSTW-- 144
            CK+  + + I G +IAP  P  W  +G   R+W+ F  +  L ++G GT+NG G  W  
Sbjct: 109 PCKA-RLTLQISGTIIAPNDPDVW--EGLNRRKWLYFHGLSRLTVEGGGTVNGMGQEWWE 168

Query: 145 -SVERNQLTELGKNFQGFILEHSEKVQIKGLTFKDSPQMHMAFGRSRNVYANNLHIQAPA 204
            S + N                 + ++++ L   DS QMH+A    R V  + L + APA
Sbjct: 169 RSCKHNHSNPCRGAPTALTFHKCKNMRVENLNVIDSQQMHIALTSCRRVTISGLKVIAPA 228

Query: 205 NSPNTDGIHIQNSQRILIRSSYIGTGDDCISIGDGSSRISISNISCGPGHGISIGSLGKH 264
            SPNTDGIHI  S+ I+I ++ + TGDDCISI   S++ISISNI CGPGHGISIGSLGK 
Sbjct: 229 TSPNTDGIHISVSRGIVIDNTTVSTGDDCISIVKNSTQISISNIICGPGHGISIGSLGKS 288

Query: 265 GQRETVEHIHVRDVTFTGTTNGARIKTWQGGRGYARHISFEGITSRGSSNPIVIDQFYCD 324
              E V  I V     + T NG RIKTWQGG G    I F  I     SNPI+IDQ+YCD
Sbjct: 289 KSWEEVRDITVDTAIISDTANGVRIKTWQGGSGLVSKIIFRNIKMNNVSNPIIIDQYYCD 348

Query: 325 -HEQCKNQTSAVKISNVLYRKISGTSGSEIAVKLACSDSIPCRNIMMKDIELIPTGLDGT 384
             + C NQTSA+ I N+ +  + GTS S+ A+K++CSDS PCRNI+++DI+L P+  DG 
Sbjct: 349 SRKPCANQTSAISIENISFVHVRGTSASKEAIKISCSDSSPCRNILLQDIDLEPSNGDG- 408

Query: 385 QSTSSFCGNVVNGLQNGTVIPSVPCLN 408
             T SFC     G  +G V P  PCL+
Sbjct: 409 -FTESFCWEAY-GSSSGQVYPP-PCLS 425

BLAST of Moc04g36150 vs. TAIR 10
Match: AT1G23460.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 303.5 bits (776), Expect = 2.6e-82
Identity = 168/402 (41.79%), Postives = 235/402 (58.46%), Query Frame = 0

Query: 30  NVINYGAVGNGATEDTQAFLNAWKATCKSNSSSSPTMVVPGGKSFLLQPFTFNGRRCKSP 89
           NV  +GA G+G ++DTQAF++AW   C   S+S    +VP G+ +L+    FNG  C+  
Sbjct: 68  NVDTFGAAGDGVSDDTQAFVSAWSKAC---STSKSVFLVPEGRRYLVNATKFNG-PCEQK 127

Query: 90  NIRIVIDGELIAPKGPSGWKYKGKKCRQWILFEHMKGLKIQGSGTINGRGSTW---SVER 149
            I I IDG ++AP  PS W  K +  R W+ F  +KG+  QG G I+G GS W   S ++
Sbjct: 128 LI-IQIDGTIVAPDEPSNWDSKFQ--RIWLEFSKLKGVVFQGKGVIDGSGSKWWAASCKK 187

Query: 150 NQLTELGKNFQGFILEHSEKVQIKGLTFKDSPQMHMAFGRSRNVYANNLHIQAPANSPNT 209
           N+            +E S  V++ GLT ++S QM+    RS +V  + + + +P +SPNT
Sbjct: 188 NKSNPCKSAPTALTIESSSGVKVSGLTIQNSQQMNFIIARSDSVRVSKVMVSSPGDSPNT 247

Query: 210 DGIHIQNSQRILIRSSYIGTGDDCISIGDGSSRISISNISCGPGHGISIGSLGKHGQRET 269
           DGIHI  S  ++++   IGTGDDC+SI + SS I + NI CGPGHGISIGSLGK      
Sbjct: 248 DGIHITGSTNVILQDCKIGTGDDCVSIVNASSNIKMKNIYCGPGHGISIGSLGKDNTTGI 307

Query: 270 VEHIHVRDVTFTGTTNGARIKTWQGGRGYARHISFEGITSRGSSNPIVIDQFYCDH-EQC 329
           V  + +       TTNG RIKT+QGG GY + I F  +  +  +NPI+IDQFYCD    C
Sbjct: 308 VTQVVLDTALLRETTNGLRIKTYQGGSGYVQGIRFTNVEMQDVANPILIDQFYCDSPTTC 367

Query: 330 KNQTSAVKISNVLYRKISGTSGSEIAVKLACSDSIPCRNIMMKDIELIPTGLDGTQSTSS 389
           +NQTSAVKIS ++YR I+GT+ S  A+K ACSD++PC +I++ ++ L   G DG     +
Sbjct: 368 QNQTSAVKISQIMYRNITGTTKSAKAIKFACSDTVPCSHIVLNNVNL--EGNDG--QVEA 427

Query: 390 FCGNVVNGLQNGTVIPSVPC--------LNQTRPRDRLELEH 420
           +C N   G   G + PS  C        L+QT   +  E  H
Sbjct: 428 YC-NSAEGFGYGVIHPSADCLYSHDDKGLDQTHKSEEAETGH 457

BLAST of Moc04g36150 vs. TAIR 10
Match: AT3G15720.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 300.8 bits (769), Expect = 1.7e-81
Identity = 166/385 (43.12%), Postives = 225/385 (58.44%), Query Frame = 0

Query: 25  SAQTFNVINYGAVGNGATEDTQAFLNAWKATCKSNSSSSPTMVVPGGKSFLLQPFTFNGR 84
           S+   +V  +GAVG+G T+D+QAFL AW+A C          VVP G +F+LQP  F G 
Sbjct: 20  SSNALDVTQFGAVGDGVTDDSQAFLKAWEAVCSGTGDGQ--FVVPAGMTFMLQPLKFQG- 79

Query: 85  RCKSPNIRIVIDGELIAPKGPSGWKYKGKKCRQWILFEHMKGLKIQGSGTINGRGSTWSV 144
            CKS  + + + G+L+A   PS   +KG K  QWILF  ++GL I+G G ING+GS+W  
Sbjct: 80  SCKSTPVFVQMLGKLVA---PSKGNWKGDK-DQWILFTDIEGLVIEGDGEINGQGSSWWE 139

Query: 145 ERNQLTELGKNFQGFILEHSEKVQIKGLTFKDSPQMHMAFGRSRNVYANNLHIQAPANSP 204
            +      G             +++ GLT  DSP  H+       V  ++L I AP +SP
Sbjct: 140 HK------GSRPTALKFRSCNNLRLSGLTHLDSPMAHIHISECNYVTISSLRINAPESSP 199

Query: 205 NTDGIHIQNSQRILIRSSYIGTGDDCISIGDGSSRISISNISCGPGHGISIGSLGKHGQR 264
           NTDGI +  S  ++I+   I TGDDCI+I  G+S I IS I CGPGHGISIGSLGK G+ 
Sbjct: 200 NTDGIDVGASSNVVIQDCIIATGDDCIAINSGTSNIHISGIDCGPGHGISIGSLGKDGET 259

Query: 265 ETVEHIHVRDVTFTGTTNGARIKTWQGGRGYARHISFEGITSRGSSNPIVIDQFY--CDH 324
            TVE++ V++  F GT NGARIKTWQGG GYAR I+F GIT     NPI+IDQFY   D 
Sbjct: 260 ATVENVCVQNCNFRGTMNGARIKTWQGGSGYARMITFNGITLDNVENPIIIDQFYNGGDS 319

Query: 325 EQCKN-QTSAVKISNVLYRKISGTSGSEIAVKLACSDSIPCRNIMMKDIELIPTGLDGTQ 384
           +  K+ ++SAV++S V++    GTS SE  V   CS+ +PC  I ++D+++        Q
Sbjct: 320 DNAKDRKSSAVEVSKVVFSNFIGTSKSEYGVDFRCSERVPCTEIFLRDMKIETASSGSGQ 379

Query: 385 STSSFCGNVVNGLQNGTVIPSVPCL 407
                C NV     +   +P + CL
Sbjct: 380 VAQGQCLNVRGA--STIAVPGLECL 389

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022133622.18.1e-22796.59probable polygalacturonase At1g80170 [Momordica charantia][more]
XP_038889205.18.6e-16073.51probable polygalacturonase At1g80170 [Benincasa hispida][more]
KGN52445.17.0e-14666.42hypothetical protein Csa_008476 [Cucumis sativus][more]
XP_031741769.12.1e-14267.18probable polygalacturonase At1g80170, partial [Cucumis sativus][more]
XP_016902407.16.2e-14264.88PREDICTED: probable polygalacturonase At1g80170 [Cucumis melo][more]
Match NameE-valueIdentityDescription
Q94AJ52.8e-8143.93Probable polygalacturonase At1g80170 OS=Arabidopsis thaliana OX=3702 GN=At1g8017... [more]
Q9LW072.4e-8043.12Probable polygalacturonase At3g15720 OS=Arabidopsis thaliana OX=3702 GN=At3g1572... [more]
Q020969.1e-8044.72Polygalacturonase OS=Persea americana OX=3435 PE=2 SV=1[more]
Q7M1E75.0e-7839.75Polygalacturonase OS=Chamaecyparis obtusa OX=13415 PE=1 SV=1[more]
Q9SFB73.2e-7743.43Polygalacturonase QRT2 OS=Arabidopsis thaliana OX=3702 GN=QRT2 PE=1 SV=2[more]
Match NameE-valueIdentityDescription
A0A6J1BX763.9e-22796.59probable polygalacturonase At1g80170 OS=Momordica charantia OX=3673 GN=LOC111006... [more]
A0A0A0KS583.4e-14666.42Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G635400 PE=3 SV=1[more]
A0A1S4E2F63.0e-14264.88probable polygalacturonase At1g80170 OS=Cucumis melo OX=3656 GN=LOC103498735 PE=... [more]
A0A2N9EX521.6e-12759.42Uncharacterized protein OS=Fagus sylvatica OX=28930 GN=FSB_LOCUS11208 PE=3 SV=1[more]
A0A1Q3BPJ53.5e-12760.67Glyco_hydro_28 domain-containing protein (Fragment) OS=Cephalotus follicularis O... [more]
Match NameE-valueIdentityDescription
AT1G56710.12.4e-8342.21Pectin lyase-like superfamily protein [more]
AT5G14650.14.1e-8342.93Pectin lyase-like superfamily protein [more]
AT1G80170.12.0e-8243.93Pectin lyase-like superfamily protein [more]
AT1G23460.12.6e-8241.79Pectin lyase-like superfamily protein [more]
AT3G15720.11.7e-8143.12Pectin lyase-like superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (OHB3-1) v2
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR006626Parallel beta-helix repeatSMARTSM00710pbh1coord: 237..257
e-value: 4200.0
score: 2.8
coord: 214..235
e-value: 1900.0
score: 5.2
coord: 296..317
e-value: 2100.0
score: 4.9
coord: 187..213
e-value: 630.0
score: 8.6
coord: 267..288
e-value: 34.0
score: 13.3
IPR012334Pectin lyase foldGENE3D2.160.20.10coord: 16..410
e-value: 1.4E-112
score: 378.7
IPR000743Glycoside hydrolase, family 28PFAMPF00295Glyco_hydro_28coord: 64..391
e-value: 1.4E-84
score: 283.9
IPR000743Glycoside hydrolase, family 28PROSITEPS00502POLYGALACTURONASEcoord: 244..257
NoneNo IPR availablePANTHERPTHR31375FAMILY NOT NAMEDcoord: 19..400
NoneNo IPR availablePANTHERPTHR31375:SF41PECTIN LYASE-LIKE SUPERFAMILY PROTEINcoord: 19..400
IPR011050Pectin lyase fold/virulence factorSUPERFAMILY51126Pectin lyase-likecoord: 13..407

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Moc04g36150.1Moc04g36150.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0005975 carbohydrate metabolic process
molecular_function GO:0004650 polygalacturonase activity