Moc04g35830 (gene) Bitter gourd (OHB3-1) v2

Overview


Name	Moc04g35830
Type	gene
Organism	Momordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
Description	metalloendoproteinase 2-MMP-like
Location	chr4: 26866840 .. 26867799 (-)
RNA-Seq Expression	Moc04g35830
Synteny	Moc04g35830

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGTCCTCCCAAGCTTTCCAAATCTTTGCTTTCATCTTATTTCTTCTTCTTTCCTTCTTCCCTCATCTAAATATGTCGTCTCGTGTACTGATTTTGAAACATAATGCCATTGCCCACGACATGAACTCATCTTTCTCATTGTCTTTTAACAATCTCCGAGGATGTCGTTTGGGTGACACAGCACAAGGCATTCATCGCCTCAAAAAGTACCTTCGACGTTTCGGTTACATTACCAATGTGGAGAAGAATTCCATGGCCATGGATGATGACACCTTCGACCATGCCTTGGAATCCGCCGTTAAAACTTACCAGAAAAATTACAATATTGATGCCTCTGGAATTTTGGACGCCAAGACCATAACCCAAATGCGCACGCCTCGATGTGGGGTTCAAGATTTCTTCAATGGAACCACTTGGATGAGATCATCAATGGACCACAAAAATAGAGATGGCCGTTTCCACACAGTTTCTCACTATTCTTTCTTCGGGGGGAAACCAAAATGGCCAGCTTCCAAGTTCCACTTGAGCTATGCATTTCTTCCGGGCTACCCATCTGAAGCAATTGAACCCGTGGATCGAGCTTTTTCCAAATGGGCTTCCGAAACCCACTTCACATTTTCAAAGATTTCGAACTATAAAAAATCCGATATCAAAATAAGCTTCGAAATTGAAGACCATGGAGATTTCGCCTCGTTCGATGGTATGGGAGGGATTCTAGCCCATGCTTTTCCACCCACGGACGGCAGACTTCACTTCGACGCTCATGAGCCATGGTCTGTGGGGGCTCTTTCTGGTTATTTTGATGTTGAAACGGTGGCGTTGCATGAGATTGGCCATCTTCTTGGGCTTCAACACAGCTCCATTGAAGCGGCTATTATGTATCCAACCATACCAGAGGGAGATACCAAGGATTTGCATGGCGATGATGTTGCTGGACTTAAGGCTTTATATGGAATTTGA

mRNA sequence

ATGTCCTCCCAAGCTTTCCAAATCTTTGCTTTCATCTTATTTCTTCTTCTTTCCTTCTTCCCTCATCTAAATATGTCGTCTCGTGTACTGATTTTGAAACATAATGCCATTGCCCACGACATGAACTCATCTTTCTCATTGTCTTTTAACAATCTCCGAGGATGTCGTTTGGGTGACACAGCACAAGGCATTCATCGCCTCAAAAAGTACCTTCGACGTTTCGGTTACATTACCAATGTGGAGAAGAATTCCATGGCCATGGATGATGACACCTTCGACCATGCCTTGGAATCCGCCGTTAAAACTTACCAGAAAAATTACAATATTGATGCCTCTGGAATTTTGGACGCCAAGACCATAACCCAAATGCGCACGCCTCGATGTGGGGTTCAAGATTTCTTCAATGGAACCACTTGGATGAGATCATCAATGGACCACAAAAATAGAGATGGCCGTTTCCACACAGTTTCTCACTATTCTTTCTTCGGGGGGAAACCAAAATGGCCAGCTTCCAAGTTCCACTTGAGCTATGCATTTCTTCCGGGCTACCCATCTGAAGCAATTGAACCCGTGGATCGAGCTTTTTCCAAATGGGCTTCCGAAACCCACTTCACATTTTCAAAGATTTCGAACTATAAAAAATCCGATATCAAAATAAGCTTCGAAATTGAAGACCATGGAGATTTCGCCTCGTTCGATGGTATGGGAGGGATTCTAGCCCATGCTTTTCCACCCACGGACGGCAGACTTCACTTCGACGCTCATGAGCCATGGTCTGTGGGGGCTCTTTCTGGTTATTTTGATGTTGAAACGGTGGCGTTGCATGAGATTGGCCATCTTCTTGGGCTTCAACACAGCTCCATTGAAGCGGCTATTATGTATCCAACCATACCAGAGGGAGATACCAAGGATTTGCATGGCGATGATGTTGCTGGACTTAAGGCTTTATATGGAATTTGA

Coding sequence (CDS)

ATGTCCTCCCAAGCTTTCCAAATCTTTGCTTTCATCTTATTTCTTCTTCTTTCCTTCTTCCCTCATCTAAATATGTCGTCTCGTGTACTGATTTTGAAACATAATGCCATTGCCCACGACATGAACTCATCTTTCTCATTGTCTTTTAACAATCTCCGAGGATGTCGTTTGGGTGACACAGCACAAGGCATTCATCGCCTCAAAAAGTACCTTCGACGTTTCGGTTACATTACCAATGTGGAGAAGAATTCCATGGCCATGGATGATGACACCTTCGACCATGCCTTGGAATCCGCCGTTAAAACTTACCAGAAAAATTACAATATTGATGCCTCTGGAATTTTGGACGCCAAGACCATAACCCAAATGCGCACGCCTCGATGTGGGGTTCAAGATTTCTTCAATGGAACCACTTGGATGAGATCATCAATGGACCACAAAAATAGAGATGGCCGTTTCCACACAGTTTCTCACTATTCTTTCTTCGGGGGGAAACCAAAATGGCCAGCTTCCAAGTTCCACTTGAGCTATGCATTTCTTCCGGGCTACCCATCTGAAGCAATTGAACCCGTGGATCGAGCTTTTTCCAAATGGGCTTCCGAAACCCACTTCACATTTTCAAAGATTTCGAACTATAAAAAATCCGATATCAAAATAAGCTTCGAAATTGAAGACCATGGAGATTTCGCCTCGTTCGATGGTATGGGAGGGATTCTAGCCCATGCTTTTCCACCCACGGACGGCAGACTTCACTTCGACGCTCATGAGCCATGGTCTGTGGGGGCTCTTTCTGGTTATTTTGATGTTGAAACGGTGGCGTTGCATGAGATTGGCCATCTTCTTGGGCTTCAACACAGCTCCATTGAAGCGGCTATTATGTATCCAACCATACCAGAGGGAGATACCAAGGATTTGCATGGCGATGATGTTGCTGGACTTAAGGCTTTATATGGAATTTGA

Protein sequence

MSSQAFQIFAFILFLLLSFFPHLNMSSRVLILKHNAIAHDMNSSFSLSFNNLRGCRLGDTAQGIHRLKKYLRRFGYITNVEKNSMAMDDDTFDHALESAVKTYQKNYNIDASGILDAKTITQMRTPRCGVQDFFNGTTWMRSSMDHKNRDGRFHTVSHYSFFGGKPKWPASKFHLSYAFLPGYPSEAIEPVDRAFSKWASETHFTFSKISNYKKSDIKISFEIEDHGDFASFDGMGGILAHAFPPTDGRLHFDAHEPWSVGALSGYFDVETVALHEIGHLLGLQHSSIEAAIMYPTIPEGDTKDLHGDDVAGLKALYGI

Homology

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BLAST of Moc04g35830 vs. NCBI nr
Match: XP_022134118.1 (metalloendoproteinase 3-MMP-like [Momordica charantia] >AKQ06192.1 metalloendoproteinase 1-like precursor [Momordica charantia])

HSP 1 Score: 656.8 bits (1693), Expect = 9.9e-185
Identity = 319/319 (100.00%), Postives = 319/319 (100.00%), Query Frame = 0


Query: 1   MSSQAFQIFAFILFLLLSFFPHLNMSSRVLILKHNAIAHDMNSSFSLSFNNLRGCRLGDT 60
           MSSQAFQIFAFILFLLLSFFPHLNMSSRVLILKHNAIAHDMNSSFSLSFNNLRGCRLGDT
Sbjct: 1   MSSQAFQIFAFILFLLLSFFPHLNMSSRVLILKHNAIAHDMNSSFSLSFNNLRGCRLGDT 60

Query: 61  AQGIHRLKKYLRRFGYITNVEKNSMAMDDDTFDHALESAVKTYQKNYNIDASGILDAKTI 120
           AQGIHRLKKYLRRFGYITNVEKNSMAMDDDTFDHALESAVKTYQKNYNIDASGILDAKTI
Sbjct: 61  AQGIHRLKKYLRRFGYITNVEKNSMAMDDDTFDHALESAVKTYQKNYNIDASGILDAKTI 120

Query: 121 TQMRTPRCGVQDFFNGTTWMRSSMDHKNRDGRFHTVSHYSFFGGKPKWPASKFHLSYAFL 180
           TQMRTPRCGVQDFFNGTTWMRSSMDHKNRDGRFHTVSHYSFFGGKPKWPASKFHLSYAFL
Sbjct: 121 TQMRTPRCGVQDFFNGTTWMRSSMDHKNRDGRFHTVSHYSFFGGKPKWPASKFHLSYAFL 180

Query: 181 PGYPSEAIEPVDRAFSKWASETHFTFSKISNYKKSDIKISFEIEDHGDFASFDGMGGILA 240
           PGYPSEAIEPVDRAFSKWASETHFTFSKISNYKKSDIKISFEIEDHGDFASFDGMGGILA
Sbjct: 181 PGYPSEAIEPVDRAFSKWASETHFTFSKISNYKKSDIKISFEIEDHGDFASFDGMGGILA 240

Query: 241 HAFPPTDGRLHFDAHEPWSVGALSGYFDVETVALHEIGHLLGLQHSSIEAAIMYPTIPEG 300
           HAFPPTDGRLHFDAHEPWSVGALSGYFDVETVALHEIGHLLGLQHSSIEAAIMYPTIPEG
Sbjct: 241 HAFPPTDGRLHFDAHEPWSVGALSGYFDVETVALHEIGHLLGLQHSSIEAAIMYPTIPEG 300

Query: 301 DTKDLHGDDVAGLKALYGI 320
           DTKDLHGDDVAGLKALYGI
Sbjct: 301 DTKDLHGDDVAGLKALYGI 319

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BLAST of Moc04g35830 vs. NCBI nr
Match: XP_022134170.1 (metalloendoproteinase 2-MMP-like [Momordica charantia])

HSP 1 Score: 542.7 bits (1397), Expect = 2.1e-150
Identity = 267/320 (83.44%), Postives = 287/320 (89.69%), Query Frame = 0


Query: 1   MSSQAFQIFAFILFLLLSFFPHLNMSSRVLILKHNAIAHDMNSSFSLSFNNLRGCRLGDT 60
           M+S+A QIFAFILFLLLSFFPHLNMSS VLILK NAIAHDMNSS SLSFNNLRGCRLGDT
Sbjct: 1   MASKALQIFAFILFLLLSFFPHLNMSSHVLILKQNAIAHDMNSSSSLSFNNLRGCRLGDT 60

Query: 61  AQGIHRLKKYLRRFGYITNVEKNSMAMDDDTFDHALESAVKTYQKNYNIDASGILDAKTI 120
           AQGIHRLKKYLRRFGYITNVEKNSMAMDDDTFDHALESAVKTYQKNYNIDASGILDAKTI
Sbjct: 61  AQGIHRLKKYLRRFGYITNVEKNSMAMDDDTFDHALESAVKTYQKNYNIDASGILDAKTI 120

Query: 121 TQMRTPRCGVQDFFNGTTWMRSSMDHKNRDG--RFHTVSHYSFFGGKPKWPASKFHLSYA 180
           TQM  PRCGVQDF NGTTWMRSS   +N++G   FH VSH++FF G  KWPASKFHLSYA
Sbjct: 121 TQMGMPRCGVQDFINGTTWMRSSGRRRNQNGHSHFHKVSHFAFFEGNLKWPASKFHLSYA 180

Query: 181 FLPGYPSEAIEPVDRAFSKWASETHFTFSKISNYKKSDIKISFEIEDHGDFASFDGMGGI 240
           FLPGYPSEAI PV RAFSKWAS THFTFS +S++K +DIKISFEIE+HGD A+FDG GGI
Sbjct: 181 FLPGYPSEAILPVSRAFSKWASNTHFTFSHVSDHKTADIKISFEIEEHGDDAAFDGAGGI 240

Query: 241 LAHAFPPTDGRLHFDAHEPWSVGALSGYFDVETVALHEIGHLLGLQHSSIEAAIMYPTIP 300
           LAHA+PPTDGR H DAHE WSVGA+SG+ DVETVALHEIGHLLGLQHSSIE  IM+P+I 
Sbjct: 241 LAHAYPPTDGRFHLDAHERWSVGAVSGHHDVETVALHEIGHLLGLQHSSIEGPIMFPSIS 300

Query: 301 EGDTKDLHGDDVAGLKALYG 319
           EG TK LHGDD+AG+KALYG
Sbjct: 301 EGVTKGLHGDDIAGIKALYG 320

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BLAST of Moc04g35830 vs. NCBI nr
Match: XP_022962668.1 (metalloendoproteinase 2-MMP-like [Cucurbita moschata])

HSP 1 Score: 431.8 bits (1109), Expect = 5.2e-117
Identity = 218/333 (65.47%), Postives = 257/333 (77.18%), Query Frame = 0


Query: 1   MSSQAFQIFAFILFLLLSFFPHLN------------MSSRVLILKHNAIAHDMNSSFSLS 60
           M+S+  Q+ AF L LL SFFPH +            + S  +IL HN  +H  NSS  L 
Sbjct: 1   MASKPLQLLAFTL-LLPSFFPHFDIVSVQCHSESEAVGSSPIILNHN--SHATNSSSLLF 60

Query: 61  FNNLRGCRLGDTAQGIHRLKKYLRRFGYITNVEKNSMAMD-DDTFDHALESAVKTYQKNY 120
            NNL+GCRLGDTAQGIHR+KKYL+RFGYITNV+K S AMD DDTFD+ALESA+KTYQKN+
Sbjct: 61  LNNLQGCRLGDTAQGIHRIKKYLQRFGYITNVQKQSNAMDHDDTFDNALESALKTYQKNF 120

Query: 121 NIDASGILDAKTITQMRTPRCGVQDFFNGTTWMRSSMDHKNR-DGRFHTVSHYSFFGGKP 180
           N+ ASGILDA T+ QM  PRCGV+D  NGTTWMRS  + K +    FH VSH++FF G  
Sbjct: 121 NLVASGILDANTLAQMGKPRCGVEDVINGTTWMRSGRERKPKMHAHFHLVSHFAFFEGNL 180

Query: 181 KWPASKFHLSYAFLPGYPSEAIEPVDRAFSKWASETHFTFSKISNYKKSDIKISFEIEDH 240
           KWP SKFHL+YAFLPGYP EA+  V RAFSKWA  THFTFS++ ++ K+DIKISFEI DH
Sbjct: 181 KWPPSKFHLTYAFLPGYPFEAMMAVSRAFSKWAFNTHFTFSRVFDHTKADIKISFEIGDH 240

Query: 241 GDFASFDGMGGILAHAFPPTDGRLHFDAHEPWSVGALSGYFDVETVALHEIGHLLGLQHS 300
           GD   FDG+GGILAHA+ PTDGRLHFD  E WSVGA+ GYFDVET+ALHEIGH+LGLQHS
Sbjct: 241 GDDVPFDGVGGILAHAYAPTDGRLHFDGDEAWSVGAVDGYFDVETIALHEIGHVLGLQHS 300

Query: 301 SIEAAIMYPTIPEGDTKDLHGDDVAGLKALYGI 320
           SIE AIM+P+I EG TK LHGDD+AG++ALY +
Sbjct: 301 SIENAIMFPSISEGVTKGLHGDDIAGIRALYKV 330

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BLAST of Moc04g35830 vs. NCBI nr
Match: XP_022991021.1 (metalloendoproteinase 2-MMP-like [Cucurbita maxima])

HSP 1 Score: 426.0 bits (1094), Expect = 2.8e-115
Identity = 215/333 (64.56%), Postives = 256/333 (76.88%), Query Frame = 0


Query: 1   MSSQAFQIFAFILFLLLSFFPHLNMS------------SRVLILKHNAIAHDMNSSFSLS 60
           M+S+  Q+ AF L LL SFFPH +++            S  +IL HN  +H  NS+  L 
Sbjct: 1   MASKPLQLLAFTL-LLPSFFPHFDIASVQCHSESKVVGSSPIILNHN--SHATNSASLLF 60

Query: 61  FNNLRGCRLGDTAQGIHRLKKYLRRFGYITNVEKNSMAMD-DDTFDHALESAVKTYQKNY 120
            NNL+GCRLGDT QGIHR+KKYL+RFGYI+NV+K+S AMD DDTFD+ LESA+KTYQKN+
Sbjct: 61  LNNLQGCRLGDTVQGIHRIKKYLQRFGYISNVQKHSNAMDHDDTFDNTLESALKTYQKNF 120

Query: 121 NIDASGILDAKTITQMRTPRCGVQDFFNGTTWMRSSMDHKNR-DGRFHTVSHYSFFGGKP 180
           N+ ASGILDA T+ QM  PRCGV+D  NGTTWMRS  +   +    FH VSH++FF G  
Sbjct: 121 NLVASGILDANTLAQMGKPRCGVEDVINGTTWMRSGRERNPKMHAHFHLVSHFAFFEGNL 180

Query: 181 KWPASKFHLSYAFLPGYPSEAIEPVDRAFSKWASETHFTFSKISNYKKSDIKISFEIEDH 240
           KWP SKFHL+YAFLPGYP EA+  V RAFSKWA  THFTFS++ ++ K+DIKISFEI DH
Sbjct: 181 KWPPSKFHLTYAFLPGYPFEAMMAVSRAFSKWAFNTHFTFSRVFDHTKADIKISFEIGDH 240

Query: 241 GDFASFDGMGGILAHAFPPTDGRLHFDAHEPWSVGALSGYFDVETVALHEIGHLLGLQHS 300
           GD A FDG GGILAHA+ PTDGRLHFD  E WSVGA+ GYFDVETVALHEIGH+LGLQHS
Sbjct: 241 GDDAPFDGAGGILAHAYAPTDGRLHFDGDEAWSVGAVDGYFDVETVALHEIGHVLGLQHS 300

Query: 301 SIEAAIMYPTIPEGDTKDLHGDDVAGLKALYGI 320
           SIE AIM+P+I EG TK LHGDD+AG++ALY +
Sbjct: 301 SIENAIMFPSISEGVTKGLHGDDIAGIRALYKV 330

[Close]

BLAST of Moc04g35830 vs. NCBI nr
Match: XP_038891016.1 (metalloendoproteinase 2-MMP-like [Benincasa hispida])

HSP 1 Score: 421.0 bits (1081), Expect = 9.1e-114
Identity = 215/321 (66.98%), Postives = 256/321 (79.75%), Query Frame = 0


Query: 2   SSQAFQIFAFILFLLLSFFPHLNMSSRVLILKHNAIAHDMNSSFSLSF--NNLRGCRLGD 61
           SS+A Q+      LLL FFPH N+S+  +ILKHN  +H+MN+S SL F   +L+GCR+GD
Sbjct: 3   SSKALQMIFPFTLLLLFFFPHPNIST-PMILKHN--SHNMNAS-SLMFLKKDLQGCRVGD 62

Query: 62  TAQGIHRLKKYLRRFGYITNVEKNS-MAMDDDTFDHALESAVKTYQKNYNIDASGILDAK 121
           T QGIH++KKYL+RFGYITN++K+S   + DDTFDH LESA+KTYQKN+N+  SGILD+ 
Sbjct: 63  TIQGIHQIKKYLQRFGYITNIKKHSNYPIFDDTFDHILESALKTYQKNHNLVPSGILDSN 122

Query: 122 TITQMRTPRCGVQDFFNGTTWMRSSMDHKNRDGRFHTVSHYSFFGGKPKWPASKFHLSYA 181
           TI QM  PRCGVQD  NGTT  R+ + +KN    FH VSH++ F G PKWP+SKFHLSYA
Sbjct: 123 TIAQMAMPRCGVQDVINGTTRKRNQI-YKNGHTHFHKVSHFTLFEGNPKWPSSKFHLSYA 182

Query: 182 FLPGYPSEAIEPVDRAFSKWASETHFTFSKISNYKKSDIKISFEIEDHGDFASFDGMGGI 241
           F PGYPSEAI  V RAFSKWA  THFTFS + +Y K+DIKISFE  +HGD ASFDG+GGI
Sbjct: 183 FPPGYPSEAIMAVSRAFSKWALNTHFTFSHVVDYNKADIKISFERGEHGDDASFDGVGGI 242

Query: 242 LAHAFPPTDGRLHFDAHEPWSVGALSGYFDVETVALHEIGHLLGLQHSSIEAAIMYPTIP 301
           LAHA+PPTDGRLHFDA EPWSVGA+SGYFDVETVALHEIGH+LGLQHSSI  AIM+P+I 
Sbjct: 243 LAHAYPPTDGRLHFDADEPWSVGAISGYFDVETVALHEIGHILGLQHSSIGTAIMFPSIS 302

Query: 302 EGDTKDLHGDDVAGLKALYGI 320
            G TK LH DD+AG+KALY +
Sbjct: 303 AGVTKGLHWDDIAGIKALYRV 318

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BLAST of Moc04g35830 vs. ExPASy Swiss-Prot
Match: Q5XF51 (Metalloendoproteinase 3-MMP OS=Arabidopsis thaliana OX=3702 GN=3MMP PE=1 SV=1)

HSP 1 Score: 227.3 bits (578), Expect = 2.5e-58
Identity = 138/335 (41.19%), Postives = 182/335 (54.33%), Query Frame = 0


Query: 7   QIFAFILFLLLSFFP-------HLNMSSRVLILKHNAIAHDMNSSFSLSFNNLRGCRLGD 66
           +I  F++FLL  F P       + N S+    L  NA  +  N     SF N  GC  G 
Sbjct: 3   RICVFMVFLLF-FAPSPVSAGFYTNSSAIPPQLLRNATGNPWN-----SFLNFTGCHAGK 62

Query: 67  TAQGIHRLKKYLRRFGYITNVEKNSMAMDDDTFDHALESAVKTYQKNYNIDASGILDAKT 126
              G++ LK+Y + FGYIT  E N      D FD  L++AV+ YQ+N+ ++ +G+LD  T
Sbjct: 63  KYDGLYMLKQYFQHFGYIT--ETNLSGNFTDDFDDILKNAVEMYQRNFQLNVTGVLDELT 122

Query: 127 ITQMRTPRCGVQDFFNGTTWMRS-----SMDHKNRDGRFHTVSHYSFFGGKPKWPASKFH 186
           +  +  PRCG  D  NGT+ M S      +    R  RFH V HYSFF G+P+WP ++  
Sbjct: 123 LKHVVIPRCGNPDVVNGTSTMHSGRKTFEVSFAGRGQRFHAVKHYSFFPGEPRWPRNRRD 182

Query: 187 LSYAFLP--GYPSEAIEPVDRAFSKWASETHFTFSKISNYKKSDIKISFEIEDHGDFASF 246
           L+YAF P      E      RAF++W   T  TF+++  +  SDI I F   +HGD   F
Sbjct: 183 LTYAFDPRNALTEEVKSVFSRAFTRWEEVTPLTFTRVERFSTSDISIGFYSGEHGDGEPF 242

Query: 247 DGMGGILAHAFPPTDGRLHFDAHEPWSVG--------ALSGYFDVETVALHEIGHLLGLQ 306
           DG    LAHAF P  G  H D  E W V         ++S   D+E+VA+HEIGHLLGL 
Sbjct: 243 DGPMRTLAHAFSPPTGHFHLDGEENWIVSGEGGDGFISVSEAVDLESVAVHEIGHLLGLG 302

Query: 307 HSSIEAAIMYPTIPEGDTK-DLHGDDVAGLKALYG 319
           HSS+E +IMYPTI  G  K DL  DDV G++ LYG
Sbjct: 303 HSSVEGSIMYPTIRTGRRKVDLTTDDVEGVQYLYG 329

[Close]

BLAST of Moc04g35830 vs. ExPASy Swiss-Prot
Match: O04529 (Metalloendoproteinase 2-MMP OS=Arabidopsis thaliana OX=3702 GN=2MMP PE=1 SV=1)

HSP 1 Score: 225.3 bits (573), Expect = 9.6e-58
Identity = 131/325 (40.31%), Postives = 183/325 (56.31%), Query Frame = 0


Query: 11  FILFLLLSFFPHLNMSSRVLILKHNAIAHDMNSS---FSLSFNNLRGCRLGDTAQGIHRL 70
           F +F  LS F  ++ +S        A+   + ++   F  +F+N  GC  G    G++R+
Sbjct: 3   FCVFGFLSLFLIVSPASAWFFPNSTAVPPSLRNTTRVFWDAFSNFTGCHHGQNVDGLYRI 62

Query: 71  KKYLRRFGYITNVEKNSMAMDDDTFDHALESAVKTYQKNYNIDASGILDAKTITQMRTPR 130
           KKY +RFGYI      +     D FD  L++AV+ YQ N+N++ +G LDA TI  +  PR
Sbjct: 63  KKYFQRFGYIPETFSGNFT---DDFDDILKAAVELYQTNFNLNVTGELDALTIQHIVIPR 122

Query: 131 CGVQDFFNGTTWM----RSSMDHKNRDGRFHTVSHYSFFGGKPKWPASKFHLSYAFLPGY 190
           CG  D  NGT+ M    R + +        H V  Y+ F G+P+WP ++  L+YAF P  
Sbjct: 123 CGNPDVVNGTSLMHGGRRKTFEVNFSRTHLHAVKRYTLFPGEPRWPRNRRDLTYAFDPKN 182

Query: 191 P-SEAIEPV-DRAFSKWASETHFTFSKISNYKKSDIKISFEIEDHGDFASFDGMGGILAH 250
           P +E ++ V  RAF +W+  T   F+   ++  SDI I F   DHGD   FDG+ G LAH
Sbjct: 183 PLTEEVKSVFSRAFGRWSDVTALNFTLSESFSTSDITIGFYTGDHGDGEPFDGVLGTLAH 242

Query: 251 AFPPTDGRLHFDAHEPWSVG-------ALSGYFDVETVALHEIGHLLGLQHSSIEAAIMY 310
           AF P  G+ H DA E W V        +++   D+E+VA+HEIGHLLGL HSS+E +IMY
Sbjct: 243 AFSPPSGKFHLDADENWVVSGDLDSFLSVTAAVDLESVAVHEIGHLLGLGHSSVEESIMY 302

Query: 311 PTIPEGDTK-DLHGDDVAGLKALYG 319
           PTI  G  K DL  DDV G++ LYG
Sbjct: 303 PTITTGKRKVDLTNDDVEGIQYLYG 324

[Close]

BLAST of Moc04g35830 vs. ExPASy Swiss-Prot
Match: Q9ZUJ5 (Metalloendoproteinase 5-MMP OS=Arabidopsis thaliana OX=3702 GN=5MMP PE=1 SV=1)

HSP 1 Score: 218.8 bits (556), Expect = 9.0e-56
Identity = 119/283 (42.05%), Postives = 159/283 (56.18%), Query Frame = 0


Query: 48  SFNNLRGCRLGDTAQGIHRLKKYLRRFGYITNVEKNSMAMDDDTFDHALESAVKTYQKNY 107
           +F+ L GC +G+   G+ +LK+Y RRFGYIT     +     D FD  L+SA+ TYQKN+
Sbjct: 44  TFSKLAGCHIGENINGLSKLKQYFRRFGYITTTGNCT-----DDFDDVLQSAINTYQKNF 103

Query: 108 NIDASGILDAKTITQMRTPRCGVQDFFNGTTWMRSSMDHKNRDGRFHTVSHYSFFGGKPK 167
           N+  +G LD+ T+ Q+  PRCG  D  +G + M       N      T   YSFF GKP+
Sbjct: 104 NLKVTGKLDSSTLRQIVKPRCGNPDLIDGVSEM-------NGGKILRTTEKYSFFPGKPR 163

Query: 168 WPASKFHLSYAFLP--GYPSEAIEPVDRAFSKWASETHFTFSKISNYKKSDIKISFEIED 227
           WP  K  L+YAF P      E      RAF++WA  T   F++  +  ++DI I F   +
Sbjct: 164 WPKRKRDLTYAFAPQNNLTDEVKRVFSRAFTRWAEVTPLNFTRSESILRADIVIGFFSGE 223

Query: 228 HGDFASFDGMGGILAHAFPPTDGRLHFDAHEPWSVG---------ALSGYFDVETVALHE 287
           HGD   FDG  G LAHA  P  G LH D  E W +           ++   D+E+VA+HE
Sbjct: 224 HGDGEPFDGAMGTLAHASSPPTGMLHLDGDEDWLISNGEISRRILPVTTVVDLESVAVHE 283

Query: 288 IGHLLGLQHSSIEAAIMYPTIPEGDTK-DLHGDDVAGLKALYG 319
           IGHLLGL HSS+E AIM+P I  GD K +L  DD+ G++ LYG
Sbjct: 284 IGHLLGLGHSSVEDAIMFPAISGGDRKVELAKDDIEGIQHLYG 314

[Close]

BLAST of Moc04g35830 vs. ExPASy Swiss-Prot
Match: P29136 (Metalloendoproteinase 1 OS=Glycine max OX=3847 PE=1 SV=2)

HSP 1 Score: 214.2 bits (544), Expect = 2.2e-54
Identity = 118/271 (43.54%), Postives = 153/271 (56.46%), Query Frame = 0


Query: 58  GDTAQGIHRLKKYLRRFGYITNVEKNSMAMDDDTFDHALESAVKTYQKNYNIDASGILDA 117
           G   +G+  +K Y    GYI N         DD FD  L SA+KTYQKNYN++ +G  D 
Sbjct: 48  GQNYKGLSNVKNYFHHLGYIPNAPH-----FDDNFDDTLVSAIKTYQKNYNLNVTGKFDI 107

Query: 118 KTITQMRTPRCGVQDFFNGTTWMRSSMDHKNRDGRFHTVSHYSFFGGKPKWPASKFHLSY 177
            T+ Q+ TPRCGV D    T          N+   F  +S Y+FF   P+W A    L+Y
Sbjct: 108 NTLKQIMTPRCGVPDIIINT----------NKTTSFGMISDYTFFKDMPRWQAGTTQLTY 167

Query: 178 AFL--PGYPSEAIEPVDRAFSKWASETHFTFSKISNYKKSDIKISFEIEDHGDFASFDGM 237
           AF   P         + RAFSKW    +  F + ++Y+ ++IKI F  ++HGD   FDG 
Sbjct: 168 AFSPEPRLDDTFKSAIARAFSKWTPVVNIAFQETTSYETANIKILFASKNHGDPYPFDGP 227

Query: 238 GGILAHAFPPTDGRLHFDAHEPW------SVGALSGYFDVETVALHEIGHLLGLQHSSIE 297
           GGIL HAF PTDGR HFDA E W      +   ++  FD+E+VA+HEIGHLLGL HSS  
Sbjct: 228 GGILGHAFAPTDGRCHFDADEYWVASGDVTKSPVTSAFDLESVAVHEIGHLLGLGHSSDL 287

Query: 298 AAIMYPTIPEGDTK-DLHGDDVAGLKALYGI 320
            AIMYP+IP    K +L  DD+ G++ LYGI
Sbjct: 288 RAIMYPSIPPRTRKVNLAQDDIDGIRKLYGI 303

[Close]

BLAST of Moc04g35830 vs. ExPASy Swiss-Prot
Match: O23507 (Metalloendoproteinase 1-MMP OS=Arabidopsis thaliana OX=3702 GN=1MMP PE=1 SV=1)

HSP 1 Score: 208.4 bits (529), Expect = 1.2e-52
Identity = 126/298 (42.28%), Postives = 169/298 (56.71%), Query Frame = 0


Query: 33  KHNAIAHDMNSSFSLSFNNLRGCRLGDTAQGIHRLKKYLRRFGYITNVEKNSMAMDDDTF 92
           K   I H  NS++   F+ L   ++G    G+  LK+YL RFGY+     +   +  D F
Sbjct: 40  KATQIIHVSNSTWH-DFSRLVDVQIGSHVSGVSELKRYLHRFGYV----NDGSEIFSDVF 99

Query: 93  DHALESAVKTYQKNYNIDASGILDAKTITQMRTPRCGVQDFFNGTTWMRSSMDHKNRDGR 152
           D  LESA+  YQ+N  +  +G LD  T+T M  PRCGV D     T M  + D       
Sbjct: 100 DGPLESAISLYQENLGLPITGRLDTSTVTLMSLPRCGVSD-----THMTINNDF------ 159

Query: 153 FHTVSHYSFFGGKPKWPASKFHLSYAF-----LPGYPSEAIEPV-DRAFSKWASETHFTF 212
            HT +HY++F GKPKW  ++  L+YA      L    SE ++ V  RAFS+W+S    +F
Sbjct: 160 LHTTAHYTYFNGKPKW--NRDTLTYAISKTHKLDYLTSEDVKTVFRRAFSQWSSVIPVSF 219

Query: 213 SKISNYKKSDIKISFEIEDHGDFASFDGMGGILAHAFPPTDGRLHFDAHEPWSV-----G 272
            ++ ++  +D+KI F   DHGD   FDG+ G LAHAF P +GRLH DA E W V     G
Sbjct: 220 EEVDDFTTADLKIGFYAGDHGDGLPFDGVLGTLAHAFAPENGRLHLDAAETWIVDDDLKG 279

Query: 273 ALSGYFDVETVALHEIGHLLGLQHSSIEAAIMYPTI-PEGDTKDLHGDDVAGLKALYG 319
           +     D+E+VA HEIGHLLGL HSS E+A+MYP++ P     DL  DDVAG+  LYG
Sbjct: 280 SSEVAVDLESVATHEIGHLLGLGHSSQESAVMYPSLRPRTKKVDLTVDDVAGVLKLYG 319

[Close]

BLAST of Moc04g35830 vs. ExPASy TrEMBL
Match: A0A0H4TG97 (Metalloendoproteinase 1-like OS=Momordica charantia OX=3673 GN=LOC111006468 PE=2 SV=1)

HSP 1 Score: 656.8 bits (1693), Expect = 4.8e-185
Identity = 319/319 (100.00%), Postives = 319/319 (100.00%), Query Frame = 0


Query: 1   MSSQAFQIFAFILFLLLSFFPHLNMSSRVLILKHNAIAHDMNSSFSLSFNNLRGCRLGDT 60
           MSSQAFQIFAFILFLLLSFFPHLNMSSRVLILKHNAIAHDMNSSFSLSFNNLRGCRLGDT
Sbjct: 1   MSSQAFQIFAFILFLLLSFFPHLNMSSRVLILKHNAIAHDMNSSFSLSFNNLRGCRLGDT 60

Query: 61  AQGIHRLKKYLRRFGYITNVEKNSMAMDDDTFDHALESAVKTYQKNYNIDASGILDAKTI 120
           AQGIHRLKKYLRRFGYITNVEKNSMAMDDDTFDHALESAVKTYQKNYNIDASGILDAKTI
Sbjct: 61  AQGIHRLKKYLRRFGYITNVEKNSMAMDDDTFDHALESAVKTYQKNYNIDASGILDAKTI 120

Query: 121 TQMRTPRCGVQDFFNGTTWMRSSMDHKNRDGRFHTVSHYSFFGGKPKWPASKFHLSYAFL 180
           TQMRTPRCGVQDFFNGTTWMRSSMDHKNRDGRFHTVSHYSFFGGKPKWPASKFHLSYAFL
Sbjct: 121 TQMRTPRCGVQDFFNGTTWMRSSMDHKNRDGRFHTVSHYSFFGGKPKWPASKFHLSYAFL 180

Query: 181 PGYPSEAIEPVDRAFSKWASETHFTFSKISNYKKSDIKISFEIEDHGDFASFDGMGGILA 240
           PGYPSEAIEPVDRAFSKWASETHFTFSKISNYKKSDIKISFEIEDHGDFASFDGMGGILA
Sbjct: 181 PGYPSEAIEPVDRAFSKWASETHFTFSKISNYKKSDIKISFEIEDHGDFASFDGMGGILA 240

Query: 241 HAFPPTDGRLHFDAHEPWSVGALSGYFDVETVALHEIGHLLGLQHSSIEAAIMYPTIPEG 300
           HAFPPTDGRLHFDAHEPWSVGALSGYFDVETVALHEIGHLLGLQHSSIEAAIMYPTIPEG
Sbjct: 241 HAFPPTDGRLHFDAHEPWSVGALSGYFDVETVALHEIGHLLGLQHSSIEAAIMYPTIPEG 300

Query: 301 DTKDLHGDDVAGLKALYGI 320
           DTKDLHGDDVAGLKALYGI
Sbjct: 301 DTKDLHGDDVAGLKALYGI 319

[Close]

BLAST of Moc04g35830 vs. ExPASy TrEMBL
Match: A0A6J1BXD5 (metalloendoproteinase 2-MMP-like OS=Momordica charantia OX=3673 GN=LOC111006498 PE=3 SV=1)

HSP 1 Score: 542.7 bits (1397), Expect = 1.0e-150
Identity = 267/320 (83.44%), Postives = 287/320 (89.69%), Query Frame = 0


Query: 1   MSSQAFQIFAFILFLLLSFFPHLNMSSRVLILKHNAIAHDMNSSFSLSFNNLRGCRLGDT 60
           M+S+A QIFAFILFLLLSFFPHLNMSS VLILK NAIAHDMNSS SLSFNNLRGCRLGDT
Sbjct: 1   MASKALQIFAFILFLLLSFFPHLNMSSHVLILKQNAIAHDMNSSSSLSFNNLRGCRLGDT 60

Query: 61  AQGIHRLKKYLRRFGYITNVEKNSMAMDDDTFDHALESAVKTYQKNYNIDASGILDAKTI 120
           AQGIHRLKKYLRRFGYITNVEKNSMAMDDDTFDHALESAVKTYQKNYNIDASGILDAKTI
Sbjct: 61  AQGIHRLKKYLRRFGYITNVEKNSMAMDDDTFDHALESAVKTYQKNYNIDASGILDAKTI 120

Query: 121 TQMRTPRCGVQDFFNGTTWMRSSMDHKNRDG--RFHTVSHYSFFGGKPKWPASKFHLSYA 180
           TQM  PRCGVQDF NGTTWMRSS   +N++G   FH VSH++FF G  KWPASKFHLSYA
Sbjct: 121 TQMGMPRCGVQDFINGTTWMRSSGRRRNQNGHSHFHKVSHFAFFEGNLKWPASKFHLSYA 180

Query: 181 FLPGYPSEAIEPVDRAFSKWASETHFTFSKISNYKKSDIKISFEIEDHGDFASFDGMGGI 240
           FLPGYPSEAI PV RAFSKWAS THFTFS +S++K +DIKISFEIE+HGD A+FDG GGI
Sbjct: 181 FLPGYPSEAILPVSRAFSKWASNTHFTFSHVSDHKTADIKISFEIEEHGDDAAFDGAGGI 240

Query: 241 LAHAFPPTDGRLHFDAHEPWSVGALSGYFDVETVALHEIGHLLGLQHSSIEAAIMYPTIP 300
           LAHA+PPTDGR H DAHE WSVGA+SG+ DVETVALHEIGHLLGLQHSSIE  IM+P+I 
Sbjct: 241 LAHAYPPTDGRFHLDAHERWSVGAVSGHHDVETVALHEIGHLLGLQHSSIEGPIMFPSIS 300

Query: 301 EGDTKDLHGDDVAGLKALYG 319
           EG TK LHGDD+AG+KALYG
Sbjct: 301 EGVTKGLHGDDIAGIKALYG 320

[Close]

BLAST of Moc04g35830 vs. ExPASy TrEMBL
Match: A0A6J1HFG1 (metalloendoproteinase 2-MMP-like OS=Cucurbita moschata OX=3662 GN=LOC111463086 PE=3 SV=1)

HSP 1 Score: 431.8 bits (1109), Expect = 2.5e-117
Identity = 218/333 (65.47%), Postives = 257/333 (77.18%), Query Frame = 0


Query: 1   MSSQAFQIFAFILFLLLSFFPHLN------------MSSRVLILKHNAIAHDMNSSFSLS 60
           M+S+  Q+ AF L LL SFFPH +            + S  +IL HN  +H  NSS  L 
Sbjct: 1   MASKPLQLLAFTL-LLPSFFPHFDIVSVQCHSESEAVGSSPIILNHN--SHATNSSSLLF 60

Query: 61  FNNLRGCRLGDTAQGIHRLKKYLRRFGYITNVEKNSMAMD-DDTFDHALESAVKTYQKNY 120
            NNL+GCRLGDTAQGIHR+KKYL+RFGYITNV+K S AMD DDTFD+ALESA+KTYQKN+
Sbjct: 61  LNNLQGCRLGDTAQGIHRIKKYLQRFGYITNVQKQSNAMDHDDTFDNALESALKTYQKNF 120

Query: 121 NIDASGILDAKTITQMRTPRCGVQDFFNGTTWMRSSMDHKNR-DGRFHTVSHYSFFGGKP 180
           N+ ASGILDA T+ QM  PRCGV+D  NGTTWMRS  + K +    FH VSH++FF G  
Sbjct: 121 NLVASGILDANTLAQMGKPRCGVEDVINGTTWMRSGRERKPKMHAHFHLVSHFAFFEGNL 180

Query: 181 KWPASKFHLSYAFLPGYPSEAIEPVDRAFSKWASETHFTFSKISNYKKSDIKISFEIEDH 240
           KWP SKFHL+YAFLPGYP EA+  V RAFSKWA  THFTFS++ ++ K+DIKISFEI DH
Sbjct: 181 KWPPSKFHLTYAFLPGYPFEAMMAVSRAFSKWAFNTHFTFSRVFDHTKADIKISFEIGDH 240

Query: 241 GDFASFDGMGGILAHAFPPTDGRLHFDAHEPWSVGALSGYFDVETVALHEIGHLLGLQHS 300
           GD   FDG+GGILAHA+ PTDGRLHFD  E WSVGA+ GYFDVET+ALHEIGH+LGLQHS
Sbjct: 241 GDDVPFDGVGGILAHAYAPTDGRLHFDGDEAWSVGAVDGYFDVETIALHEIGHVLGLQHS 300

Query: 301 SIEAAIMYPTIPEGDTKDLHGDDVAGLKALYGI 320
           SIE AIM+P+I EG TK LHGDD+AG++ALY +
Sbjct: 301 SIENAIMFPSISEGVTKGLHGDDIAGIRALYKV 330

[Close]

BLAST of Moc04g35830 vs. ExPASy TrEMBL
Match: A0A6J1JPK1 (metalloendoproteinase 2-MMP-like OS=Cucurbita maxima OX=3661 GN=LOC111487736 PE=3 SV=1)

HSP 1 Score: 426.0 bits (1094), Expect = 1.4e-115
Identity = 215/333 (64.56%), Postives = 256/333 (76.88%), Query Frame = 0


Query: 1   MSSQAFQIFAFILFLLLSFFPHLNMS------------SRVLILKHNAIAHDMNSSFSLS 60
           M+S+  Q+ AF L LL SFFPH +++            S  +IL HN  +H  NS+  L 
Sbjct: 1   MASKPLQLLAFTL-LLPSFFPHFDIASVQCHSESKVVGSSPIILNHN--SHATNSASLLF 60

Query: 61  FNNLRGCRLGDTAQGIHRLKKYLRRFGYITNVEKNSMAMD-DDTFDHALESAVKTYQKNY 120
            NNL+GCRLGDT QGIHR+KKYL+RFGYI+NV+K+S AMD DDTFD+ LESA+KTYQKN+
Sbjct: 61  LNNLQGCRLGDTVQGIHRIKKYLQRFGYISNVQKHSNAMDHDDTFDNTLESALKTYQKNF 120

Query: 121 NIDASGILDAKTITQMRTPRCGVQDFFNGTTWMRSSMDHKNR-DGRFHTVSHYSFFGGKP 180
           N+ ASGILDA T+ QM  PRCGV+D  NGTTWMRS  +   +    FH VSH++FF G  
Sbjct: 121 NLVASGILDANTLAQMGKPRCGVEDVINGTTWMRSGRERNPKMHAHFHLVSHFAFFEGNL 180

Query: 181 KWPASKFHLSYAFLPGYPSEAIEPVDRAFSKWASETHFTFSKISNYKKSDIKISFEIEDH 240
           KWP SKFHL+YAFLPGYP EA+  V RAFSKWA  THFTFS++ ++ K+DIKISFEI DH
Sbjct: 181 KWPPSKFHLTYAFLPGYPFEAMMAVSRAFSKWAFNTHFTFSRVFDHTKADIKISFEIGDH 240

Query: 241 GDFASFDGMGGILAHAFPPTDGRLHFDAHEPWSVGALSGYFDVETVALHEIGHLLGLQHS 300
           GD A FDG GGILAHA+ PTDGRLHFD  E WSVGA+ GYFDVETVALHEIGH+LGLQHS
Sbjct: 241 GDDAPFDGAGGILAHAYAPTDGRLHFDGDEAWSVGAVDGYFDVETVALHEIGHVLGLQHS 300

Query: 301 SIEAAIMYPTIPEGDTKDLHGDDVAGLKALYGI 320
           SIE AIM+P+I EG TK LHGDD+AG++ALY +
Sbjct: 301 SIENAIMFPSISEGVTKGLHGDDIAGIRALYKV 330

[Close]

BLAST of Moc04g35830 vs. ExPASy TrEMBL
Match: A0A1S3C814 (metalloendoproteinase 1-like OS=Cucumis melo OX=3656 GN=LOC103498042 PE=3 SV=1)

HSP 1 Score: 401.4 bits (1030), Expect = 3.6e-108
Identity = 203/321 (63.24%), Postives = 247/321 (76.95%), Query Frame = 0


Query: 2   SSQAFQIFAFILFLLLSFFPHLNMSSRVLILKHNAIAHDMNSSFSLSF-NNLRGCRLGDT 61
           SS+A QI      L LS FP+LN SS  +ILKHN  +H+MNSS SL F  NL+GC LGDT
Sbjct: 3   SSKAIQIIFPFTLLFLSLFPNLNTSS-PMILKHN--SHNMNSSNSLMFLKNLQGCHLGDT 62

Query: 62  AQGIHRLKKYLRRFGYI-TNVEKNSMAMDDDTFDHALESAVKTYQKNYNIDASGILDAKT 121
            QGI ++KKYL+RFGYI TN++K+S  + DDTFD  LESA+KTYQ N+N+  SGILD+ T
Sbjct: 63  KQGIQQIKKYLQRFGYITTNIQKHSNPIFDDTFDQVLESALKTYQTNHNLAPSGILDSNT 122

Query: 122 ITQMRTPRCGVQDFFNGTTWMRSSMDHKNRDG-RFHTVSHYSFFGGKPKWPASKFHLSYA 181
           + Q+  PRCGVQD        + + ++KN     FH VSH++FF G  KWP+SKFHLSY 
Sbjct: 123 LAQIAKPRCGVQDVIKN---KKRNQNYKNNGHIHFHKVSHFTFFEGNLKWPSSKFHLSYG 182

Query: 182 FLPGYPSEAIEPVDRAFSKWASETHFTFSKISNYKKSDIKISFEIEDHGDFASFDGMGGI 241
           FLP YP EAI+PV RAFSKW+  THF FS + +Y+K+DIKISFE  +HGD A FDG+GG+
Sbjct: 183 FLPNYPIEAIKPVSRAFSKWSLNTHFKFSHVVDYRKADIKISFERGEHGDNAPFDGVGGV 242

Query: 242 LAHAFPPTDGRLHFDAHEPWSVGALSGYFDVETVALHEIGHLLGLQHSSIEAAIMYPTIP 301
           LAHA+PPTDGRLHFD  E WSVGA+SGYFDVETVALHEIGH+LGLQHS+IE AIM+P+IP
Sbjct: 243 LAHAYPPTDGRLHFDGDEAWSVGAISGYFDVETVALHEIGHILGLQHSTIEEAIMFPSIP 302

Query: 302 EGDTKDLHGDDVAGLKALYGI 320
           EG TK LHGDD+AG+K LY +
Sbjct: 303 EGVTKGLHGDDIAGIKTLYRV 317

[Close]

BLAST of Moc04g35830 vs. TAIR 10
Match: AT1G24140.1 (Matrixin family protein )

HSP 1 Score: 227.3 bits (578), Expect = 1.8e-59
Identity = 138/335 (41.19%), Postives = 182/335 (54.33%), Query Frame = 0


Query: 7   QIFAFILFLLLSFFP-------HLNMSSRVLILKHNAIAHDMNSSFSLSFNNLRGCRLGD 66
           +I  F++FLL  F P       + N S+    L  NA  +  N     SF N  GC  G 
Sbjct: 3   RICVFMVFLLF-FAPSPVSAGFYTNSSAIPPQLLRNATGNPWN-----SFLNFTGCHAGK 62

Query: 67  TAQGIHRLKKYLRRFGYITNVEKNSMAMDDDTFDHALESAVKTYQKNYNIDASGILDAKT 126
              G++ LK+Y + FGYIT  E N      D FD  L++AV+ YQ+N+ ++ +G+LD  T
Sbjct: 63  KYDGLYMLKQYFQHFGYIT--ETNLSGNFTDDFDDILKNAVEMYQRNFQLNVTGVLDELT 122

Query: 127 ITQMRTPRCGVQDFFNGTTWMRS-----SMDHKNRDGRFHTVSHYSFFGGKPKWPASKFH 186
           +  +  PRCG  D  NGT+ M S      +    R  RFH V HYSFF G+P+WP ++  
Sbjct: 123 LKHVVIPRCGNPDVVNGTSTMHSGRKTFEVSFAGRGQRFHAVKHYSFFPGEPRWPRNRRD 182

Query: 187 LSYAFLP--GYPSEAIEPVDRAFSKWASETHFTFSKISNYKKSDIKISFEIEDHGDFASF 246
           L+YAF P      E      RAF++W   T  TF+++  +  SDI I F   +HGD   F
Sbjct: 183 LTYAFDPRNALTEEVKSVFSRAFTRWEEVTPLTFTRVERFSTSDISIGFYSGEHGDGEPF 242

Query: 247 DGMGGILAHAFPPTDGRLHFDAHEPWSVG--------ALSGYFDVETVALHEIGHLLGLQ 306
           DG    LAHAF P  G  H D  E W V         ++S   D+E+VA+HEIGHLLGL 
Sbjct: 243 DGPMRTLAHAFSPPTGHFHLDGEENWIVSGEGGDGFISVSEAVDLESVAVHEIGHLLGLG 302

Query: 307 HSSIEAAIMYPTIPEGDTK-DLHGDDVAGLKALYG 319
           HSS+E +IMYPTI  G  K DL  DDV G++ LYG
Sbjct: 303 HSSVEGSIMYPTIRTGRRKVDLTTDDVEGVQYLYG 329

[Close]

BLAST of Moc04g35830 vs. TAIR 10
Match: AT1G70170.1 (matrix metalloproteinase )

HSP 1 Score: 225.3 bits (573), Expect = 6.8e-59
Identity = 131/325 (40.31%), Postives = 183/325 (56.31%), Query Frame = 0


Query: 11  FILFLLLSFFPHLNMSSRVLILKHNAIAHDMNSS---FSLSFNNLRGCRLGDTAQGIHRL 70
           F +F  LS F  ++ +S        A+   + ++   F  +F+N  GC  G    G++R+
Sbjct: 3   FCVFGFLSLFLIVSPASAWFFPNSTAVPPSLRNTTRVFWDAFSNFTGCHHGQNVDGLYRI 62

Query: 71  KKYLRRFGYITNVEKNSMAMDDDTFDHALESAVKTYQKNYNIDASGILDAKTITQMRTPR 130
           KKY +RFGYI      +     D FD  L++AV+ YQ N+N++ +G LDA TI  +  PR
Sbjct: 63  KKYFQRFGYIPETFSGNFT---DDFDDILKAAVELYQTNFNLNVTGELDALTIQHIVIPR 122

Query: 131 CGVQDFFNGTTWM----RSSMDHKNRDGRFHTVSHYSFFGGKPKWPASKFHLSYAFLPGY 190
           CG  D  NGT+ M    R + +        H V  Y+ F G+P+WP ++  L+YAF P  
Sbjct: 123 CGNPDVVNGTSLMHGGRRKTFEVNFSRTHLHAVKRYTLFPGEPRWPRNRRDLTYAFDPKN 182

Query: 191 P-SEAIEPV-DRAFSKWASETHFTFSKISNYKKSDIKISFEIEDHGDFASFDGMGGILAH 250
           P +E ++ V  RAF +W+  T   F+   ++  SDI I F   DHGD   FDG+ G LAH
Sbjct: 183 PLTEEVKSVFSRAFGRWSDVTALNFTLSESFSTSDITIGFYTGDHGDGEPFDGVLGTLAH 242

Query: 251 AFPPTDGRLHFDAHEPWSVG-------ALSGYFDVETVALHEIGHLLGLQHSSIEAAIMY 310
           AF P  G+ H DA E W V        +++   D+E+VA+HEIGHLLGL HSS+E +IMY
Sbjct: 243 AFSPPSGKFHLDADENWVVSGDLDSFLSVTAAVDLESVAVHEIGHLLGLGHSSVEESIMY 302

Query: 311 PTIPEGDTK-DLHGDDVAGLKALYG 319
           PTI  G  K DL  DDV G++ LYG
Sbjct: 303 PTITTGKRKVDLTNDDVEGIQYLYG 324

[Close]

BLAST of Moc04g35830 vs. TAIR 10
Match: AT1G59970.1 (Matrixin family protein )

HSP 1 Score: 218.8 bits (556), Expect = 6.4e-57
Identity = 119/283 (42.05%), Postives = 159/283 (56.18%), Query Frame = 0


Query: 48  SFNNLRGCRLGDTAQGIHRLKKYLRRFGYITNVEKNSMAMDDDTFDHALESAVKTYQKNY 107
           +F+ L GC +G+   G+ +LK+Y RRFGYIT     +     D FD  L+SA+ TYQKN+
Sbjct: 44  TFSKLAGCHIGENINGLSKLKQYFRRFGYITTTGNCT-----DDFDDVLQSAINTYQKNF 103

Query: 108 NIDASGILDAKTITQMRTPRCGVQDFFNGTTWMRSSMDHKNRDGRFHTVSHYSFFGGKPK 167
           N+  +G LD+ T+ Q+  PRCG  D  +G + M       N      T   YSFF GKP+
Sbjct: 104 NLKVTGKLDSSTLRQIVKPRCGNPDLIDGVSEM-------NGGKILRTTEKYSFFPGKPR 163

Query: 168 WPASKFHLSYAFLP--GYPSEAIEPVDRAFSKWASETHFTFSKISNYKKSDIKISFEIED 227
           WP  K  L+YAF P      E      RAF++WA  T   F++  +  ++DI I F   +
Sbjct: 164 WPKRKRDLTYAFAPQNNLTDEVKRVFSRAFTRWAEVTPLNFTRSESILRADIVIGFFSGE 223

Query: 228 HGDFASFDGMGGILAHAFPPTDGRLHFDAHEPWSVG---------ALSGYFDVETVALHE 287
           HGD   FDG  G LAHA  P  G LH D  E W +           ++   D+E+VA+HE
Sbjct: 224 HGDGEPFDGAMGTLAHASSPPTGMLHLDGDEDWLISNGEISRRILPVTTVVDLESVAVHE 283

Query: 288 IGHLLGLQHSSIEAAIMYPTIPEGDTK-DLHGDDVAGLKALYG 319
           IGHLLGL HSS+E AIM+P I  GD K +L  DD+ G++ LYG
Sbjct: 284 IGHLLGLGHSSVEDAIMFPAISGGDRKVELAKDDIEGIQHLYG 314

[Close]

BLAST of Moc04g35830 vs. TAIR 10
Match: AT4G16640.1 (Matrixin family protein )

HSP 1 Score: 208.4 bits (529), Expect = 8.6e-54
Identity = 126/298 (42.28%), Postives = 169/298 (56.71%), Query Frame = 0


Query: 33  KHNAIAHDMNSSFSLSFNNLRGCRLGDTAQGIHRLKKYLRRFGYITNVEKNSMAMDDDTF 92
           K   I H  NS++   F+ L   ++G    G+  LK+YL RFGY+     +   +  D F
Sbjct: 40  KATQIIHVSNSTWH-DFSRLVDVQIGSHVSGVSELKRYLHRFGYV----NDGSEIFSDVF 99

Query: 93  DHALESAVKTYQKNYNIDASGILDAKTITQMRTPRCGVQDFFNGTTWMRSSMDHKNRDGR 152
           D  LESA+  YQ+N  +  +G LD  T+T M  PRCGV D     T M  + D       
Sbjct: 100 DGPLESAISLYQENLGLPITGRLDTSTVTLMSLPRCGVSD-----THMTINNDF------ 159

Query: 153 FHTVSHYSFFGGKPKWPASKFHLSYAF-----LPGYPSEAIEPV-DRAFSKWASETHFTF 212
            HT +HY++F GKPKW  ++  L+YA      L    SE ++ V  RAFS+W+S    +F
Sbjct: 160 LHTTAHYTYFNGKPKW--NRDTLTYAISKTHKLDYLTSEDVKTVFRRAFSQWSSVIPVSF 219

Query: 213 SKISNYKKSDIKISFEIEDHGDFASFDGMGGILAHAFPPTDGRLHFDAHEPWSV-----G 272
            ++ ++  +D+KI F   DHGD   FDG+ G LAHAF P +GRLH DA E W V     G
Sbjct: 220 EEVDDFTTADLKIGFYAGDHGDGLPFDGVLGTLAHAFAPENGRLHLDAAETWIVDDDLKG 279

Query: 273 ALSGYFDVETVALHEIGHLLGLQHSSIEAAIMYPTI-PEGDTKDLHGDDVAGLKALYG 319
           +     D+E+VA HEIGHLLGL HSS E+A+MYP++ P     DL  DDVAG+  LYG
Sbjct: 280 SSEVAVDLESVATHEIGHLLGLGHSSQESAVMYPSLRPRTKKVDLTVDDVAGVLKLYG 319

[Close]

BLAST of Moc04g35830 vs. TAIR 10
Match: AT2G45040.1 (Matrixin family protein )

HSP 1 Score: 170.6 bits (431), Expect = 2.0e-42
Identity = 111/312 (35.58%), Postives = 163/312 (52.24%), Query Frame = 0


Query: 19  FFPHLNMSSRVLILKHNAIAHDMNSSFSLSFNNLRGCRLGDTAQGIHRLKKYLRRFGYIT 78
           F  +LN+ ++ +I   N      N S  +S               I  +K++L+++GY+ 
Sbjct: 20  FLLYLNLHNQQIIEARNPSQFTTNPSPDVS---------------IPEIKRHLQQYGYLP 79

Query: 79  NVEKNSMAMDDDTFDHALESAVKTYQKNYNIDASGILDAKTITQMRTPRCGVQDFFNGTT 138
             +++    DD +F    E A+  YQKN  +  +G  D+ T++Q+  PRCG  D      
Sbjct: 80  QNKES----DDVSF----EQALVRYQKNLGLPITGKPDSDTLSQILLPRCGFPD------ 139

Query: 139 WMRSSMDHKNRDGRFHTVSHYSFFGGKPKWPAS-KFHLSYAFLPGYPSEAIEPVD----- 198
                 D + +   FHT   Y +F G+P+W       L+YAF     +  + P D     
Sbjct: 140 ------DVEPKTAPFHTGKKYVYFPGRPRWTRDVPLKLTYAFSQENLTPYLAPTDIRRVF 199

Query: 199 -RAFSKWASETHFTFSKISNYKKSDIKISFEIEDHGDFASFDGMGGILAHAFPPTDGRLH 258
            RAF KWAS    +F +  +Y  +DIKI F   DHGD   FDG+ G+LAH F P +GRLH
Sbjct: 200 RRAFGKWASVIPVSFIETEDYVIADIKIGFFNGDHGDGEPFDGVLGVLAHTFSPENGRLH 259

Query: 259 FDAHEPWSVG----ALSGYFDVETVALHEIGHLLGLQHSSIEAAIMYPTI-PEGDTKDLH 318
            D  E W+V       S   D+E+VA+HEIGH+LGL HSS++ A MYPT+ P     +L+
Sbjct: 260 LDKAETWAVDFDEEKSSVAVDLESVAVHEIGHVLGLGHSSVKDAAMYPTLKPRSKKVNLN 296

The following BLAST results are available for this feature:

BLAST of Moc04g35830 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022134118.1	9.9e-185	100.00	metalloendoproteinase 3-MMP-like [Momordica charantia] >AKQ06192.1 metalloendopr...	[more]
XP_022134170.1	2.1e-150	83.44	metalloendoproteinase 2-MMP-like [Momordica charantia]	[more]
XP_022962668.1	5.2e-117	65.47	metalloendoproteinase 2-MMP-like [Cucurbita moschata]	[more]
XP_022991021.1	2.8e-115	64.56	metalloendoproteinase 2-MMP-like [Cucurbita maxima]	[more]
XP_038891016.1	9.1e-114	66.98	metalloendoproteinase 2-MMP-like [Benincasa hispida]	[more]

BLAST of Moc04g35830 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q5XF51	2.5e-58	41.19	Metalloendoproteinase 3-MMP OS=Arabidopsis thaliana OX=3702 GN=3MMP PE=1 SV=1	[more]
O04529	9.6e-58	40.31	Metalloendoproteinase 2-MMP OS=Arabidopsis thaliana OX=3702 GN=2MMP PE=1 SV=1	[more]
Q9ZUJ5	9.0e-56	42.05	Metalloendoproteinase 5-MMP OS=Arabidopsis thaliana OX=3702 GN=5MMP PE=1 SV=1	[more]
P29136	2.2e-54	43.54	Metalloendoproteinase 1 OS=Glycine max OX=3847 PE=1 SV=2	[more]
O23507	1.2e-52	42.28	Metalloendoproteinase 1-MMP OS=Arabidopsis thaliana OX=3702 GN=1MMP PE=1 SV=1	[more]

BLAST of Moc04g35830 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0H4TG97	4.8e-185	100.00	Metalloendoproteinase 1-like OS=Momordica charantia OX=3673 GN=LOC111006468 PE=2...	[more]
A0A6J1BXD5	1.0e-150	83.44	metalloendoproteinase 2-MMP-like OS=Momordica charantia OX=3673 GN=LOC111006498 ...	[more]
A0A6J1HFG1	2.5e-117	65.47	metalloendoproteinase 2-MMP-like OS=Cucurbita moschata OX=3662 GN=LOC111463086 P...	[more]
A0A6J1JPK1	1.4e-115	64.56	metalloendoproteinase 2-MMP-like OS=Cucurbita maxima OX=3661 GN=LOC111487736 PE=...	[more]
A0A1S3C814	3.6e-108	63.24	metalloendoproteinase 1-like OS=Cucumis melo OX=3656 GN=LOC103498042 PE=3 SV=1	[more]

BLAST of Moc04g35830 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G24140.1	1.8e-59	41.19	Matrixin family protein	[more]
AT1G70170.1	6.8e-59	40.31	matrix metalloproteinase	[more]
AT1G59970.1	6.4e-57	42.05	Matrixin family protein	[more]
AT4G16640.1	8.6e-54	42.28	Matrixin family protein	[more]
AT2G45040.1	2.0e-42	35.58	Matrixin family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (OHB3-1) v2
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR021190	Peptidase M10A	PRINTS	PR00138	MATRIXIN	coord: 272..297 score: 58.02 coord: 123..136 score: 52.35 coord: 215..243 score: 56.73 coord: 191..206 score: 35.21 coord: 305..318 score: 52.61
IPR006026	Peptidase, metallopeptidase	SMART	SM00235	col_5	coord: 164..319 e-value: 4.2E-28 score: 109.4
IPR002477	Peptidoglycan binding-like	PFAM	PF01471	PG_binding_1	coord: 62..123 e-value: 3.4E-12 score: 46.4
IPR001818	Peptidase M10, metallopeptidase	PFAM	PF00413	Peptidase_M10	coord: 186..318 e-value: 3.6E-42 score: 143.9
IPR024079	Metallopeptidase, catalytic domain superfamily	GENE3D	3.40.390.10	Collagenase (Catalytic Domain)	coord: 54..319 e-value: 2.4E-71 score: 242.1
None	No IPR available	PANTHER	PTHR10201	MATRIX METALLOPROTEINASE	coord: 12..317
None	No IPR available	PANTHER	PTHR10201:SF269	METALLOENDOPROTEINASE 3-MMP-LIKE	coord: 12..317
None	No IPR available	SUPERFAMILY	55486	Metalloproteases ("zincins"), catalytic domain	coord: 161..318
IPR033739	Peptidase M10A, catalytic domain	CDD	cd04278	ZnMc_MMP	coord: 167..318 e-value: 7.49058E-56 score: 176.626
IPR036365	PGBD-like superfamily	SUPERFAMILY	47090	PGBD-like	coord: 55..132

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Moc04g35830.1	Moc04g35830.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0030574	collagen catabolic process
biological_process	GO:0030198	extracellular matrix organization
biological_process	GO:0006508	proteolysis
cellular_component	GO:0031225	anchored component of membrane
cellular_component	GO:0031012	extracellular matrix
molecular_function	GO:0004222	metalloendopeptidase activity
molecular_function	GO:0008270	zinc ion binding
molecular_function	GO:0008237	metallopeptidase activity