Moc04g35750 (gene) Bitter gourd (OHB3-1) v2

Overview


Name	Moc04g35750
Type	gene
Organism	Momordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
Description	metalloendoproteinase 1-like
Location	chr4: 26836286 .. 26837285 (+)
RNA-Seq Expression	Moc04g35750
Synteny	Moc04g35750

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCCGAGTTCAAATATTCTCTTCACCTCTCCTTGATCTCTTTCATCCTCCTGGCCGCCATTGCAGCTCACGTGACCTCGGCACGTGTTCTTCATAATCACGCCCACAACAAATCATCTCATATCATATTCTCTCAAAACCTTCTAGGAAGTCGCAAGGGCCACAACATACAAGGAATCCACCACGTCAAAACTTACCTCAAACGCTATGGCTACTTCACCCACACCCAGTCAAACGCCTTTGACGATCACTTGGAATCCGCCCTCAAAACATACCAAAAATTCTTCAACCTCAACGTGAGTGGAATTCTGGACAATGAAACTTTAAGTCTAATGTCCCAGCCCCGATGTGCAGTTCCAGATATTTTCCACAATGAGACGAGTACAAGGTACTAACATCTCAAAACAAATTCAACTCAAAAGACTAGTCCATGTCTATTATAAATCTTTTGTAACATCTCTTAGGAAGGGATGGGGCCACGAGGAGCATCTCCATATGGGTTCTCACTACTTGTTCTTCCCGAGGACCCCAAAATGGCCCGAGTCCAAATGCCGTCTGCGCTACTCATATTACCACGATTTCCCAATGGCGTTCAAGGACCCAGTGAACAATGCTTTAGAGCTGTGGTCGGCGTACTTGCAGTTCGGGTTCGTGGAAGTCGGGGAGGACGATTATTGGGCAGACTTGAAGATAAGCTTCGAGATGGGGGAGCATGGAGATGGGCATCCTTTCATTCCGACGTCGGGAGTTTTGGCACATGCGTTTGCGCCGCGGGATGGGAGATTTCACTTCAACGTGGAGCAGTGCTGGACGGTGGGGGCTCGCCCAAATTGTCAAGATGTGATGTCGGTGGCAGTGCATGAGATTGGGCATCTTCTTGGGCTCGGCCACAGCGGCGCCGAGGAAGCCATCATGTGGCCATGCATACGCCCCAACGCTGTCAAGGGTTTGTTTCAAGACAATGTTGCTGGAATCAAGGCTTTGTATGGATTGCCTTAA

mRNA sequence

ATGGCCGAGTTCAAATATTCTCTTCACCTCTCCTTGATCTCTTTCATCCTCCTGGCCGCCATTGCAGCTCACGTGACCTCGGCACGTGTTCTTCATAATCACGCCCACAACAAATCATCTCATATCATATTCTCTCAAAACCTTCTAGGAAGTCGCAAGGGCCACAACATACAAGGAATCCACCACGTCAAAACTTACCTCAAACGCTATGGCTACTTCACCCACACCCAGTCAAACGCCTTTGACGATCACTTGGAATCCGCCCTCAAAACATACCAAAAATTCTTCAACCTCAACGTGAGTGGAATTCTGGACAATGAAACTTTAAGTCTAATGTCCCAGCCCCGATGTGCAGTTCCAGATATTTTCCACAATGAGACGAGTACAAGGAAGGGATGGGGCCACGAGGAGCATCTCCATATGGGTTCTCACTACTTGTTCTTCCCGAGGACCCCAAAATGGCCCGAGTCCAAATGCCGTCTGCGCTACTCATATTACCACGATTTCCCAATGGCGTTCAAGGACCCAGTGAACAATGCTTTAGAGCTGTGGTCGGCGTACTTGCAGTTCGGGTTCGTGGAAGTCGGGGAGGACGATTATTGGGCAGACTTGAAGATAAGCTTCGAGATGGGGGAGCATGGAGATGGGCATCCTTTCATTCCGACGTCGGGAGTTTTGGCACATGCGTTTGCGCCGCGGGATGGGAGATTTCACTTCAACGTGGAGCAGTGCTGGACGGTGGGGGCTCGCCCAAATTGTCAAGATGTGATGTCGGTGGCAGTGCATGAGATTGGGCATCTTCTTGGGCTCGGCCACAGCGGCGCCGAGGAAGCCATCATGTGGCCATGCATACGCCCCAACGCTGTCAAGGGTTTGTTTCAAGACAATGTTGCTGGAATCAAGGCTTTGTATGGATTGCCTTAA

Coding sequence (CDS)

ATGGCCGAGTTCAAATATTCTCTTCACCTCTCCTTGATCTCTTTCATCCTCCTGGCCGCCATTGCAGCTCACGTGACCTCGGCACGTGTTCTTCATAATCACGCCCACAACAAATCATCTCATATCATATTCTCTCAAAACCTTCTAGGAAGTCGCAAGGGCCACAACATACAAGGAATCCACCACGTCAAAACTTACCTCAAACGCTATGGCTACTTCACCCACACCCAGTCAAACGCCTTTGACGATCACTTGGAATCCGCCCTCAAAACATACCAAAAATTCTTCAACCTCAACGTGAGTGGAATTCTGGACAATGAAACTTTAAGTCTAATGTCCCAGCCCCGATGTGCAGTTCCAGATATTTTCCACAATGAGACGAGTACAAGGAAGGGATGGGGCCACGAGGAGCATCTCCATATGGGTTCTCACTACTTGTTCTTCCCGAGGACCCCAAAATGGCCCGAGTCCAAATGCCGTCTGCGCTACTCATATTACCACGATTTCCCAATGGCGTTCAAGGACCCAGTGAACAATGCTTTAGAGCTGTGGTCGGCGTACTTGCAGTTCGGGTTCGTGGAAGTCGGGGAGGACGATTATTGGGCAGACTTGAAGATAAGCTTCGAGATGGGGGAGCATGGAGATGGGCATCCTTTCATTCCGACGTCGGGAGTTTTGGCACATGCGTTTGCGCCGCGGGATGGGAGATTTCACTTCAACGTGGAGCAGTGCTGGACGGTGGGGGCTCGCCCAAATTGTCAAGATGTGATGTCGGTGGCAGTGCATGAGATTGGGCATCTTCTTGGGCTCGGCCACAGCGGCGCCGAGGAAGCCATCATGTGGCCATGCATACGCCCCAACGCTGTCAAGGGTTTGTTTCAAGACAATGTTGCTGGAATCAAGGCTTTGTATGGATTGCCTTAA

Protein sequence

MAEFKYSLHLSLISFILLAAIAAHVTSARVLHNHAHNKSSHIIFSQNLLGSRKGHNIQGIHHVKTYLKRYGYFTHTQSNAFDDHLESALKTYQKFFNLNVSGILDNETLSLMSQPRCAVPDIFHNETSTRKGWGHEEHLHMGSHYLFFPRTPKWPESKCRLRYSYYHDFPMAFKDPVNNALELWSAYLQFGFVEVGEDDYWADLKISFEMGEHGDGHPFIPTSGVLAHAFAPRDGRFHFNVEQCWTVGARPNCQDVMSVAVHEIGHLLGLGHSGAEEAIMWPCIRPNAVKGLFQDNVAGIKALYGLP

Homology

[Close]

BLAST of Moc04g35750 vs. NCBI nr
Match: XP_022133610.1 (metalloendoproteinase 1-like [Momordica charantia])

HSP 1 Score: 654.4 bits (1687), Expect = 4.7e-184
Identity = 307/307 (100.00%), Postives = 307/307 (100.00%), Query Frame = 0


Query: 1   MAEFKYSLHLSLISFILLAAIAAHVTSARVLHNHAHNKSSHIIFSQNLLGSRKGHNIQGI 60
           MAEFKYSLHLSLISFILLAAIAAHVTSARVLHNHAHNKSSHIIFSQNLLGSRKGHNIQGI
Sbjct: 1   MAEFKYSLHLSLISFILLAAIAAHVTSARVLHNHAHNKSSHIIFSQNLLGSRKGHNIQGI 60

Query: 61  HHVKTYLKRYGYFTHTQSNAFDDHLESALKTYQKFFNLNVSGILDNETLSLMSQPRCAVP 120
           HHVKTYLKRYGYFTHTQSNAFDDHLESALKTYQKFFNLNVSGILDNETLSLMSQPRCAVP
Sbjct: 61  HHVKTYLKRYGYFTHTQSNAFDDHLESALKTYQKFFNLNVSGILDNETLSLMSQPRCAVP 120

Query: 121 DIFHNETSTRKGWGHEEHLHMGSHYLFFPRTPKWPESKCRLRYSYYHDFPMAFKDPVNNA 180
           DIFHNETSTRKGWGHEEHLHMGSHYLFFPRTPKWPESKCRLRYSYYHDFPMAFKDPVNNA
Sbjct: 121 DIFHNETSTRKGWGHEEHLHMGSHYLFFPRTPKWPESKCRLRYSYYHDFPMAFKDPVNNA 180

Query: 181 LELWSAYLQFGFVEVGEDDYWADLKISFEMGEHGDGHPFIPTSGVLAHAFAPRDGRFHFN 240
           LELWSAYLQFGFVEVGEDDYWADLKISFEMGEHGDGHPFIPTSGVLAHAFAPRDGRFHFN
Sbjct: 181 LELWSAYLQFGFVEVGEDDYWADLKISFEMGEHGDGHPFIPTSGVLAHAFAPRDGRFHFN 240

Query: 241 VEQCWTVGARPNCQDVMSVAVHEIGHLLGLGHSGAEEAIMWPCIRPNAVKGLFQDNVAGI 300
           VEQCWTVGARPNCQDVMSVAVHEIGHLLGLGHSGAEEAIMWPCIRPNAVKGLFQDNVAGI
Sbjct: 241 VEQCWTVGARPNCQDVMSVAVHEIGHLLGLGHSGAEEAIMWPCIRPNAVKGLFQDNVAGI 300

Query: 301 KALYGLP 308
           KALYGLP
Sbjct: 301 KALYGLP 307

[Close]

BLAST of Moc04g35750 vs. NCBI nr
Match: XP_022133608.1 (matrix metalloproteinase-9-like [Momordica charantia])

HSP 1 Score: 379.0 bits (972), Expect = 3.8e-101
Identity = 196/309 (63.43%), Postives = 222/309 (71.84%), Query Frame = 0


Query: 1   MAEFKYSLHLSLISFILLAAIAAHVTSARVLHNHAHNKSSHIIFSQNLLGSRKGHNIQGI 60
           MAEFK+SL LSLISFILLA+IAAHVTSAR +HNH+H+KSSHIIFSQNLLG+RK HNI+GI
Sbjct: 1   MAEFKHSLQLSLISFILLASIAAHVTSARDIHNHSHDKSSHIIFSQNLLGTRKSHNIEGI 60

Query: 61  HHVKTYLKRYGYFTH--TQSNAFDDHLESALKTYQKFFNLNVSGILDNETLSLMSQPRCA 120
           H VKTYLKRYGY TH  +Q  AFDD LESALKTYQKFFNLNV+GILD ETLSLMSQPRC 
Sbjct: 61  HDVKTYLKRYGYLTHIGSQPGAFDDDLESALKTYQKFFNLNVTGILDKETLSLMSQPRCG 120

Query: 121 VPDIFHNETSTRKGWGHEEHLHMGSHYLFFPRTPKWPESKCRLRYSYYHDFPMAFKDPVN 180
           VPDIF   ++TR+     E                                   FKDPV 
Sbjct: 121 VPDIFQIFSTTRRSISTWE----------------------------------GFKDPVR 180

Query: 181 NALELWSAYLQFGFVEVGEDDYWADLKISFEMGEHGDGHPFIPTSGVLAHAFAPRDGRFH 240
            AL LWSAY  FGF E G D+   D+KISFE G HGD HPF+P +GVLAHAFAP DGRFH
Sbjct: 181 KALILWSAYSHFGFTEAGRDEL-DDIKISFERGAHGDRHPFVPGTGVLAHAFAPTDGRFH 240

Query: 241 FNVEQCWTVGARPNCQDVMSVAVHEIGHLLGLGHSGAEEAIMWPCIRPNAVKGLFQDNVA 300
            NV+Q W+VGA+    DV SVA+HEIGHLLGL HS  +EAIMWP I  N VKGL+ D++A
Sbjct: 241 LNVDQKWSVGAQEGYYDVQSVALHEIGHLLGLAHSKTKEAIMWPVIPTNFVKGLYYDDIA 274

Query: 301 GIKALYGLP 308
           G++ALYG P
Sbjct: 301 GLEALYGFP 274

[Close]

BLAST of Moc04g35750 vs. NCBI nr
Match: XP_023544311.1 (metalloendoproteinase 1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 322.4 bits (825), Expect = 4.2e-84
Identity = 169/296 (57.09%), Postives = 202/296 (68.24%), Query Frame = 0


Query: 13  ISFILLAAIAAHVTSARVLHNHAHNKSSHIIFSQNLLGSRKGHNIQGIHHVKTYLKRYGY 72
           + FIL+AAIA H   AR LH H   K S  IF Q+L+GS KGHNI+GIH+V+ +L+RYGY
Sbjct: 9   VMFILVAAIAPHAIMARHLHTH---KLSSSIFPQHLVGSGKGHNIEGIHNVRAFLQRYGY 68

Query: 73  FT---HTQSNAFDDHLESALKTYQKFFNLNVSGILDNETLSLMSQPRCAVPDIFHNETST 132
            +    T+ NAFDD+LE ALK+YQKFFNLNVSGILD ETL LMSQPRC VPD+F NET  
Sbjct: 69  LSTNVDTKGNAFDDNLEFALKSYQKFFNLNVSGILDAETLELMSQPRCGVPDVFINETG- 128

Query: 133 RKGWGHEEHLHMGSHYLFF-PRTPKWPESKCRLRYSYYHDFPMAFKDPVNNALELWSAYL 192
             G G  +H  +G HY FF    PKW  SK  L+Y++   FP  +K  V  A+  WS   
Sbjct: 129 GDGNGDRDH-PIGLHYRFFNDSKPKWSASKYNLKYTFVRSFPEQYKSTVVQAMISWSLNS 188

Query: 193 QFGFVEVGEDDYWADLKISFEMGEHGDGHPFIPTSGVLAHAFAPRDGRFHFNVEQCWTVG 252
            F F E   DD   D++ISFE+GEHGDGHPF   SGVLAHAFAP DGR HFN    W  G
Sbjct: 189 VFAFSEGSTDD--TDVRISFEVGEHGDGHPFKKGSGVLAHAFAPEDGRLHFNEWHAWADG 248

Query: 253 ARPNCQDVMSVAVHEIGHLLGLGHSGAEEAIMWPCIRPNAVKGLFQDNVAGIKALY 305
           A P+  DV SVA+HE+GH+LGLGHS   +A+MWP +RPN  K L  D+V+GI ALY
Sbjct: 249 AYPDKYDVGSVALHELGHILGLGHSDIRDAVMWPSMRPNTKKELSSDDVSGISALY 297

[Close]

BLAST of Moc04g35750 vs. NCBI nr
Match: XP_023544665.1 (metalloendoproteinase 1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 315.1 bits (806), Expect = 6.8e-82
Identity = 164/296 (55.41%), Postives = 202/296 (68.24%), Query Frame = 0


Query: 13  ISFILLAAIAAHVTSARVLHNHAHNKSSHIIFSQNLLGSRKGHNIQGIHHVKTYLKRYGY 72
           + F+L+AAIA H   AR LH H   K S  IF Q+L+GS KGHNI+GIH+V+ +L+RYGY
Sbjct: 9   VMFVLVAAIAPHAIMARHLHTH---KLSSSIFPQHLVGSGKGHNIEGIHNVRAFLQRYGY 68

Query: 73  FT---HTQSNAFDDHLESALKTYQKFFNLNVSGILDNETLSLMSQPRCAVPDIFHNETST 132
            +    T+ NAFDD+LE ALK+YQKFFNLNVSGILD ETL LMSQPRC VPD+F NETS 
Sbjct: 69  LSTNVETKGNAFDDNLEFALKSYQKFFNLNVSGILDAETLHLMSQPRCGVPDVFINETS- 128

Query: 133 RKGWGHEEHLHMGSHYLFFPR-TPKWPESKCRLRYSYYHDFPMAFKDPVNNALELWSAYL 192
             G G  +H  +G  Y FF    PKW  SK  L+Y++  +FP  +K  V  A   WS+  
Sbjct: 129 --GEGDRDH-SIGLDYTFFHEWKPKWRASKYNLKYAFVGNFPEQYKATVREATNTWSSVT 188

Query: 193 QFGFVEVGEDDYWADLKISFEMGEHGDGHPFIPTSGVLAHAFAPRDGRFHFNVEQCWTVG 252
            F F E   D+   D+KISFE+G+HGDG+PF   SGVLAHAFAP DGR H+N ++ W  G
Sbjct: 189 GFTFTEGSADN--TDVKISFEVGDHGDGYPFEDGSGVLAHAFAPEDGRLHYNAQEAWADG 248

Query: 253 ARPNCQDVMSVAVHEIGHLLGLGHSGAEEAIMWPCIRPNAVKGLFQDNVAGIKALY 305
           A P+  DV +VA+HE+GH+LGLGHS    A+MWP   PN V GL  D+V GI ALY
Sbjct: 249 AYPDKYDVGTVALHELGHVLGLGHSAFRYAVMWPITPPNFVTGLDNDDVNGIMALY 295

[Close]

BLAST of Moc04g35750 vs. NCBI nr
Match: KAG6602464.1 (hypothetical protein SDJN03_07697, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 313.9 bits (803), Expect = 1.5e-81
Identity = 165/296 (55.74%), Postives = 200/296 (67.57%), Query Frame = 0


Query: 13  ISFILLAAIAAHVTSARVLHNHAHNKSSHIIFSQNLLGSRKGHNIQGIHHVKTYLKRYGY 72
           + FIL+AAIA H   AR LH H   K S  IF Q+L+GS KGHN++GIH+V+ YL+RYGY
Sbjct: 9   VMFILVAAIAPHAIMARHLHTH---KFSSSIFPQHLVGSGKGHNMEGIHNVRAYLQRYGY 68

Query: 73  FT---HTQSNAFDDHLESALKTYQKFFNLNVSGILDNETLSLMSQPRCAVPDIFHNETST 132
            +    T+ NAFDD+LE ALK+YQKFFNLNVSGILD ETL LMSQPRC VPD+F NET  
Sbjct: 69  LSTNVDTKGNAFDDNLEFALKSYQKFFNLNVSGILDAETLELMSQPRCGVPDVFSNETG- 128

Query: 133 RKGWGHEEHLHMGSHYLFFPRT-PKWPESKCRLRYSYYHDFPMAFKDPVNNALELWSAYL 192
             G G  +H  +G  Y FF  + PKW  SK  L+Y++  +FP  +K  V  A   WS+  
Sbjct: 129 GGGDGDWDH-SIGIDYTFFRESKPKWRASKYNLKYAFVGNFPEQYKATVREATNTWSSVT 188

Query: 193 QFGFVEVGEDDYWADLKISFEMGEHGDGHPFIPTSGVLAHAFAPRDGRFHFNVEQCWTVG 252
            F F E   D+   D+KISFE+G+HGDG+PF+  SGVLAHAFAP DGR HFN    W  G
Sbjct: 189 GFTFTEGSADN--TDVKISFEVGDHGDGYPFVEGSGVLAHAFAPEDGRLHFNTRHAWADG 248

Query: 253 ARPNCQDVMSVAVHEIGHLLGLGHSGAEEAIMWPCIRPNAVKGLFQDNVAGIKALY 305
           A P   DV +VA+HE+GH+LGLGHS    A+MWP   PN V GL  D+V GI ALY
Sbjct: 249 AYPYKYDVGTVALHELGHVLGLGHSAFRYAVMWPYTPPNFVTGLDNDDVNGIMALY 297

[Close]

BLAST of Moc04g35750 vs. ExPASy Swiss-Prot
Match: O04529 (Metalloendoproteinase 2-MMP OS=Arabidopsis thaliana OX=3702 GN=2MMP PE=1 SV=1)

HSP 1 Score: 188.3 bits (477), Expect = 1.3e-46
Identity = 109/281 (38.79%), Postives = 147/281 (52.31%), Query Frame = 0


Query: 47  NLLGSRKGHNIQGIHHVKTYLKRYGYFTHTQS----NAFDDHLESALKTYQKFFNLNVSG 106
           N  G   G N+ G++ +K Y +R+GY   T S    + FDD L++A++ YQ  FNLNV+G
Sbjct: 46  NFTGCHHGQNVDGLYRIKKYFQRFGYIPETFSGNFTDDFDDILKAAVELYQTNFNLNVTG 105

Query: 107 ILDNETLSLMSQPRCAVPDIFHNETSTRKGWGHE--------EHLHMGSHYLFFPRTPKW 166
            LD  T+  +  PRC  PD+  N TS   G   +         HLH    Y  FP  P+W
Sbjct: 106 ELDALTIQHIVIPRCGNPDVV-NGTSLMHGGRRKTFEVNFSRTHLHAVKRYTLFPGEPRW 165

Query: 167 PESKCRLRYSYYHDFPMA--FKDPVNNALELWSAYLQFGFVEVGEDDYWADLKISFEMGE 226
           P ++  L Y++    P+    K   + A   WS      F  + E    +D+ I F  G+
Sbjct: 166 PRNRRDLTYAFDPKNPLTEEVKSVFSRAFGRWSDVTALNFT-LSESFSTSDITIGFYTGD 225

Query: 227 HGDGHPFIPTSGVLAHAFAPRDGRFHFNVEQCWTVGARPN-------CQDVMSVAVHEIG 286
           HGDG PF    G LAHAF+P  G+FH + ++ W V    +         D+ SVAVHEIG
Sbjct: 226 HGDGEPFDGVLGTLAHAFSPPSGKFHLDADENWVVSGDLDSFLSVTAAVDLESVAVHEIG 285

Query: 287 HLLGLGHSGAEEAIMWPCIRPNAVK-GLFQDNVAGIKALYG 306
           HLLGLGHS  EE+IM+P I     K  L  D+V GI+ LYG
Sbjct: 286 HLLGLGHSSVEESIMYPTITTGKRKVDLTNDDVEGIQYLYG 324

[Close]

BLAST of Moc04g35750 vs. ExPASy Swiss-Prot
Match: P29136 (Metalloendoproteinase 1 OS=Glycine max OX=3847 PE=1 SV=2)

HSP 1 Score: 185.3 bits (469), Expect = 1.1e-45
Identity = 106/264 (40.15%), Postives = 138/264 (52.27%), Query Frame = 0


Query: 54  GHNIQGIHHVKTYLKRYGYFTHTQ--SNAFDDHLESALKTYQKFFNLNVSGILDNETLSL 113
           G N +G+ +VK Y    GY  +     + FDD L SA+KTYQK +NLNV+G  D  TL  
Sbjct: 48  GQNYKGLSNVKNYFHHLGYIPNAPHFDDNFDDTLVSAIKTYQKNYNLNVTGKFDINTLKQ 107

Query: 114 MSQPRCAVPDIFHNETSTRKGWGHEEHLHMGSHYLFFPRTPKWPESKCRLRYSYYHD--F 173
           +  PRC VPDI  N   T           M S Y FF   P+W     +L Y++  +   
Sbjct: 108 IMTPRCGVPDIIINTNKTTS-------FGMISDYTFFKDMPRWQAGTTQLTYAFSPEPRL 167

Query: 174 PMAFKDPVNNALELWSAYLQFGFVEVGEDDYWADLKISFEMGEHGDGHPFIPTSGVLAHA 233
              FK  +  A   W+  +   F E    +  A++KI F    HGD +PF    G+L HA
Sbjct: 168 DDTFKSAIARAFSKWTPVVNIAFQETTSYE-TANIKILFASKNHGDPYPFDGPGGILGHA 227

Query: 234 FAPRDGRFHFNVEQCW------TVGARPNCQDVMSVAVHEIGHLLGLGHSGAEEAIMWPC 293
           FAP DGR HF+ ++ W      T     +  D+ SVAVHEIGHLLGLGHS    AIM+P 
Sbjct: 228 FAPTDGRCHFDADEYWVASGDVTKSPVTSAFDLESVAVHEIGHLLGLGHSSDLRAIMYPS 287

Query: 294 IRPNAVK-GLFQDNVAGIKALYGL 307
           I P   K  L QD++ GI+ LYG+
Sbjct: 288 IPPRTRKVNLAQDDIDGIRKLYGI 303

[Close]

BLAST of Moc04g35750 vs. ExPASy Swiss-Prot
Match: O23507 (Metalloendoproteinase 1-MMP OS=Arabidopsis thaliana OX=3702 GN=1MMP PE=1 SV=1)

HSP 1 Score: 184.5 bits (467), Expect = 1.8e-45
Identity = 110/285 (38.60%), Postives = 145/285 (50.88%), Query Frame = 0


Query: 34  HAHNKSSHIIFSQNLLGSRKGHNIQGIHHVKTYLKRYGYF---THTQSNAFDDHLESALK 93
           H  N + H      L+  + G ++ G+  +K YL R+GY    +   S+ FD  LESA+ 
Sbjct: 46  HVSNSTWHDF--SRLVDVQIGSHVSGVSELKRYLHRFGYVNDGSEIFSDVFDGPLESAIS 105

Query: 94  TYQKFFNLNVSGILDNETLSLMSQPRCAVPDIFHNETSTRKGWGHEEHLHMGSHYLFFPR 153
            YQ+   L ++G LD  T++LMS PRC V D             + + LH  +HY +F  
Sbjct: 106 LYQENLGLPITGRLDTSTVTLMSLPRCGVSDTHMTI--------NNDFLHTTAHYTYFNG 165

Query: 154 TPKWPESKCRLRYSYYHDFPMAFKDPV----NNALELWSAYLQFGFVEVGEDDYWADLKI 213
            PKW         S  H       + V      A   WS+ +   F EV +D   ADLKI
Sbjct: 166 KPKWNRDTLTYAISKTHKLDYLTSEDVKTVFRRAFSQWSSVIPVSFEEV-DDFTTADLKI 225

Query: 214 SFEMGEHGDGHPFIPTSGVLAHAFAPRDGRFHFNVEQCWTV-----GARPNCQDVMSVAV 273
            F  G+HGDG PF    G LAHAFAP +GR H +  + W V     G+     D+ SVA 
Sbjct: 226 GFYAGDHGDGLPFDGVLGTLAHAFAPENGRLHLDAAETWIVDDDLKGSSEVAVDLESVAT 285

Query: 274 HEIGHLLGLGHSGAEEAIMWPCIRPNAVK-GLFQDNVAGIKALYG 306
           HEIGHLLGLGHS  E A+M+P +RP   K  L  D+VAG+  LYG
Sbjct: 286 HEIGHLLGLGHSSQESAVMYPSLRPRTKKVDLTVDDVAGVLKLYG 319

[Close]

BLAST of Moc04g35750 vs. ExPASy Swiss-Prot
Match: Q5XF51 (Metalloendoproteinase 3-MMP OS=Arabidopsis thaliana OX=3702 GN=3MMP PE=1 SV=1)

HSP 1 Score: 181.8 bits (460), Expect = 1.2e-44
Identity = 104/283 (36.75%), Postives = 142/283 (50.18%), Query Frame = 0


Query: 47  NLLGSRKGHNIQGIHHVKTYLKRYGYFTHTQ-----SNAFDDHLESALKTYQKFFNLNVS 106
           N  G   G    G++ +K Y + +GY T T      ++ FDD L++A++ YQ+ F LNV+
Sbjct: 48  NFTGCHAGKKYDGLYMLKQYFQHFGYITETNLSGNFTDDFDDILKNAVEMYQRNFQLNVT 107

Query: 107 GILDNETLSLMSQPRCAVPDIFHNETSTRKG--------WGHEEHLHMGSHYLFFPRTPK 166
           G+LD  TL  +  PRC  PD+ +  ++   G         G  +  H   HY FFP  P+
Sbjct: 108 GVLDELTLKHVVIPRCGNPDVVNGTSTMHSGRKTFEVSFAGRGQRFHAVKHYSFFPGEPR 167

Query: 167 WPESKCRLRYSY--YHDFPMAFKDPVNNALELWSAYLQFGFVEVGEDDYWADLKISFEMG 226
           WP ++  L Y++   +      K   + A   W       F  V E    +D+ I F  G
Sbjct: 168 WPRNRRDLTYAFDPRNALTEEVKSVFSRAFTRWEEVTPLTFTRV-ERFSTSDISIGFYSG 227

Query: 227 EHGDGHPFIPTSGVLAHAFAPRDGRFHFNVEQCWTVGAR--------PNCQDVMSVAVHE 286
           EHGDG PF      LAHAF+P  G FH + E+ W V               D+ SVAVHE
Sbjct: 228 EHGDGEPFDGPMRTLAHAFSPPTGHFHLDGEENWIVSGEGGDGFISVSEAVDLESVAVHE 287

Query: 287 IGHLLGLGHSGAEEAIMWPCIRPNAVK-GLFQDNVAGIKALYG 306
           IGHLLGLGHS  E +IM+P IR    K  L  D+V G++ LYG
Sbjct: 288 IGHLLGLGHSSVEGSIMYPTIRTGRRKVDLTTDDVEGVQYLYG 329

[Close]

BLAST of Moc04g35750 vs. ExPASy Swiss-Prot
Match: Q9ZUJ5 (Metalloendoproteinase 5-MMP OS=Arabidopsis thaliana OX=3702 GN=5MMP PE=1 SV=1)

HSP 1 Score: 180.3 bits (456), Expect = 3.4e-44
Identity = 106/272 (38.97%), Postives = 142/272 (52.21%), Query Frame = 0


Query: 48  LLGSRKGHNIQGIHHVKTYLKRYGYFTHTQ--SNAFDDHLESALKTYQKFFNLNVSGILD 107
           L G   G NI G+  +K Y +R+GY T T   ++ FDD L+SA+ TYQK FNL V+G LD
Sbjct: 48  LAGCHIGENINGLSKLKQYFRRFGYITTTGNCTDDFDDVLQSAINTYQKNFNLKVTGKLD 107

Query: 108 NETLSLMSQPRCAVPDIFHNETSTRKGWGHEEHLHMGSHYLFFPRTPKWPESKCRLRYSY 167
           + TL  + +PRC  PD+    +    G    + L     Y FFP  P+WP+ K  L Y++
Sbjct: 108 SSTLRQIVKPRCGNPDLIDGVSEMNGG----KILRTTEKYSFFPGKPRWPKRKRDLTYAF 167

Query: 168 --YHDFPMAFKDPVNNALELWSAYLQFGFVEVGEDDYWADLKISFEMGEHGDGHPFIPTS 227
              ++     K   + A   W+      F    E    AD+ I F  GEHGDG PF    
Sbjct: 168 APQNNLTDEVKRVFSRAFTRWAEVTPLNFTR-SESILRADIVIGFFSGEHGDGEPFDGAM 227

Query: 228 GVLAHAFAPRDGRFHFNVEQCWTVG---------ARPNCQDVMSVAVHEIGHLLGLGHSG 287
           G LAHA +P  G  H + ++ W +                D+ SVAVHEIGHLLGLGHS 
Sbjct: 228 GTLAHASSPPTGMLHLDGDEDWLISNGEISRRILPVTTVVDLESVAVHEIGHLLGLGHSS 287

Query: 288 AEEAIMWPCIRPNAVK-GLFQDNVAGIKALYG 306
            E+AIM+P I     K  L +D++ GI+ LYG
Sbjct: 288 VEDAIMFPAISGGDRKVELAKDDIEGIQHLYG 314

[Close]

BLAST of Moc04g35750 vs. ExPASy TrEMBL
Match: A0A6J1BVR3 (metalloendoproteinase 1-like OS=Momordica charantia OX=3673 GN=LOC111006149 PE=3 SV=1)

HSP 1 Score: 654.4 bits (1687), Expect = 2.3e-184
Identity = 307/307 (100.00%), Postives = 307/307 (100.00%), Query Frame = 0


Query: 1   MAEFKYSLHLSLISFILLAAIAAHVTSARVLHNHAHNKSSHIIFSQNLLGSRKGHNIQGI 60
           MAEFKYSLHLSLISFILLAAIAAHVTSARVLHNHAHNKSSHIIFSQNLLGSRKGHNIQGI
Sbjct: 1   MAEFKYSLHLSLISFILLAAIAAHVTSARVLHNHAHNKSSHIIFSQNLLGSRKGHNIQGI 60

Query: 61  HHVKTYLKRYGYFTHTQSNAFDDHLESALKTYQKFFNLNVSGILDNETLSLMSQPRCAVP 120
           HHVKTYLKRYGYFTHTQSNAFDDHLESALKTYQKFFNLNVSGILDNETLSLMSQPRCAVP
Sbjct: 61  HHVKTYLKRYGYFTHTQSNAFDDHLESALKTYQKFFNLNVSGILDNETLSLMSQPRCAVP 120

Query: 121 DIFHNETSTRKGWGHEEHLHMGSHYLFFPRTPKWPESKCRLRYSYYHDFPMAFKDPVNNA 180
           DIFHNETSTRKGWGHEEHLHMGSHYLFFPRTPKWPESKCRLRYSYYHDFPMAFKDPVNNA
Sbjct: 121 DIFHNETSTRKGWGHEEHLHMGSHYLFFPRTPKWPESKCRLRYSYYHDFPMAFKDPVNNA 180

Query: 181 LELWSAYLQFGFVEVGEDDYWADLKISFEMGEHGDGHPFIPTSGVLAHAFAPRDGRFHFN 240
           LELWSAYLQFGFVEVGEDDYWADLKISFEMGEHGDGHPFIPTSGVLAHAFAPRDGRFHFN
Sbjct: 181 LELWSAYLQFGFVEVGEDDYWADLKISFEMGEHGDGHPFIPTSGVLAHAFAPRDGRFHFN 240

Query: 241 VEQCWTVGARPNCQDVMSVAVHEIGHLLGLGHSGAEEAIMWPCIRPNAVKGLFQDNVAGI 300
           VEQCWTVGARPNCQDVMSVAVHEIGHLLGLGHSGAEEAIMWPCIRPNAVKGLFQDNVAGI
Sbjct: 241 VEQCWTVGARPNCQDVMSVAVHEIGHLLGLGHSGAEEAIMWPCIRPNAVKGLFQDNVAGI 300

Query: 301 KALYGLP 308
           KALYGLP
Sbjct: 301 KALYGLP 307

[Close]

BLAST of Moc04g35750 vs. ExPASy TrEMBL
Match: A0A6J1BZL4 (matrix metalloproteinase-9-like OS=Momordica charantia OX=3673 GN=LOC111006147 PE=3 SV=1)

HSP 1 Score: 379.0 bits (972), Expect = 1.8e-101
Identity = 196/309 (63.43%), Postives = 222/309 (71.84%), Query Frame = 0


Query: 1   MAEFKYSLHLSLISFILLAAIAAHVTSARVLHNHAHNKSSHIIFSQNLLGSRKGHNIQGI 60
           MAEFK+SL LSLISFILLA+IAAHVTSAR +HNH+H+KSSHIIFSQNLLG+RK HNI+GI
Sbjct: 1   MAEFKHSLQLSLISFILLASIAAHVTSARDIHNHSHDKSSHIIFSQNLLGTRKSHNIEGI 60

Query: 61  HHVKTYLKRYGYFTH--TQSNAFDDHLESALKTYQKFFNLNVSGILDNETLSLMSQPRCA 120
           H VKTYLKRYGY TH  +Q  AFDD LESALKTYQKFFNLNV+GILD ETLSLMSQPRC 
Sbjct: 61  HDVKTYLKRYGYLTHIGSQPGAFDDDLESALKTYQKFFNLNVTGILDKETLSLMSQPRCG 120

Query: 121 VPDIFHNETSTRKGWGHEEHLHMGSHYLFFPRTPKWPESKCRLRYSYYHDFPMAFKDPVN 180
           VPDIF   ++TR+     E                                   FKDPV 
Sbjct: 121 VPDIFQIFSTTRRSISTWE----------------------------------GFKDPVR 180

Query: 181 NALELWSAYLQFGFVEVGEDDYWADLKISFEMGEHGDGHPFIPTSGVLAHAFAPRDGRFH 240
            AL LWSAY  FGF E G D+   D+KISFE G HGD HPF+P +GVLAHAFAP DGRFH
Sbjct: 181 KALILWSAYSHFGFTEAGRDEL-DDIKISFERGAHGDRHPFVPGTGVLAHAFAPTDGRFH 240

Query: 241 FNVEQCWTVGARPNCQDVMSVAVHEIGHLLGLGHSGAEEAIMWPCIRPNAVKGLFQDNVA 300
            NV+Q W+VGA+    DV SVA+HEIGHLLGL HS  +EAIMWP I  N VKGL+ D++A
Sbjct: 241 LNVDQKWSVGAQEGYYDVQSVALHEIGHLLGLAHSKTKEAIMWPVIPTNFVKGLYYDDIA 274

Query: 301 GIKALYGLP 308
           G++ALYG P
Sbjct: 301 GLEALYGFP 274

[Close]

BLAST of Moc04g35750 vs. ExPASy TrEMBL
Match: A0A6J1H7I1 (metalloendoproteinase 1-like OS=Cucurbita moschata OX=3662 GN=LOC111460787 PE=3 SV=1)

HSP 1 Score: 308.1 bits (788), Expect = 4.0e-80
Identity = 161/296 (54.39%), Postives = 200/296 (67.57%), Query Frame = 0


Query: 13  ISFILLAAIAAHVTSARVLHNHAHNKSSHIIFSQNLLGSRKGHNIQGIHHVKTYLKRYGY 72
           + FIL+AAIA H   AR LH H   K S  IF Q+L+GS KGHNI+GIH+V+ +L+RYGY
Sbjct: 9   VMFILVAAIAPHAIMARHLHIH---KLSSSIFPQHLVGSGKGHNIEGIHNVRAFLQRYGY 68

Query: 73  FT---HTQSNAFDDHLESALKTYQKFFNLNVSGILDNETLSLMSQPRCAVPDIFHNETST 132
            +    T+ N FDD+LE A+K+YQKFFNLNVSGILD ETL LMSQPRC VPD+F NET  
Sbjct: 69  LSTNVETKGNVFDDNLEFAIKSYQKFFNLNVSGILDAETLELMSQPRCGVPDVFINETG- 128

Query: 133 RKGWGHEEHLHMGSHYLFF-PRTPKWPESKCRLRYSYYHDFPMAFKDPVNNALELWSAYL 192
             G G  +H  +G HY FF    PKW  SK  L+Y++   FP  +K+ V  A+  WS   
Sbjct: 129 GDGNGDRDH-PIGLHYSFFNDLKPKWSASKYNLKYTFLGSFPEQYKNTVIQAMISWSFNS 188

Query: 193 QFGFVEVGEDDYWADLKISFEMGEHGDGHPFIPTSGVLAHAFAPRDGRFHFNVEQCWTVG 252
            F F E   D+   D++ISFE+G+HGDG PF   +G LAHAFAP DGR HFN +  W  G
Sbjct: 189 VFAFSEGSTDN--TDVRISFEVGDHGDGSPFKDKNGALAHAFAPEDGRLHFNAQHAWADG 248

Query: 253 ARPNCQDVMSVAVHEIGHLLGLGHSGAEEAIMWPCIRPNAVKGLFQDNVAGIKALY 305
           A P+  DV SVA+HE+GH+LGLGHS   +A+MWP + PN  K L  D+V+GI ALY
Sbjct: 249 AYPDKFDVGSVALHELGHILGLGHSDIRDAVMWPSMPPNLKKELSSDDVSGISALY 297

[Close]

BLAST of Moc04g35750 vs. ExPASy TrEMBL
Match: A0A6J1H5Y2 (metalloendoproteinase 1-like OS=Cucurbita moschata OX=3662 GN=LOC111460835 PE=3 SV=1)

HSP 1 Score: 302.0 bits (772), Expect = 2.9e-78
Identity = 156/283 (55.12%), Postives = 190/283 (67.14%), Query Frame = 0


Query: 28  ARVLHNHAHNKSSHIIFSQNLLGSRKGHNIQGIHHVKTYLKRYGYFT---HTQSNAFDDH 87
           AR LH H   K S  IF Q+L+GS KGHNI+GIH+V+ +L+RYGY +    T+ NAFDD+
Sbjct: 2   ARHLHTH---KLSSSIFPQHLVGSGKGHNIEGIHNVRAFLQRYGYLSTNVETKGNAFDDN 61

Query: 88  LESALKTYQKFFNLNVSGILDNETLSLMSQPRCAVPDIFHNETS--TRKGWGHEEHLHMG 147
           LE ALK+YQKFFNLNVSGILD ETL LMSQPRC VPD+F NETS    + W H     +G
Sbjct: 62  LEFALKSYQKFFNLNVSGILDAETLHLMSQPRCGVPDVFINETSGDRDRDWDHS----IG 121

Query: 148 SHYLFFPR-TPKWPESKCRLRYSYYHDFPMAFKDPVNNALELWSAYLQFGFVEVGEDDYW 207
             Y FF    PKW  SK  L+Y++  +FP  +K  V  A   WS+   F F E   D+  
Sbjct: 122 LDYTFFHEWKPKWRASKYNLKYAFVGNFPEQYKATVREATNTWSSVTGFTFTEGSADN-- 181

Query: 208 ADLKISFEMGEHGDGHPFIPTSGVLAHAFAPRDGRFHFNVEQCWTVGARPNCQDVMSVAV 267
            D+KISFE+G+HGDG+PF   SGVLAHAFAP DGR H+N  + W  GA P+  DV +VA+
Sbjct: 182 TDVKISFEVGDHGDGYPFEEGSGVLAHAFAPEDGRLHYNAREAWADGAYPDKYDVGTVAL 241

Query: 268 HEIGHLLGLGHSGAEEAIMWPCIRPNAVKGLFQDNVAGIKALY 305
           HE+GH+LGLGHS    A+MWP   PN V GL  D+V GI ALY
Sbjct: 242 HELGHVLGLGHSAFRYAVMWPITPPNFVTGLDNDDVNGIMALY 275

[Close]

BLAST of Moc04g35750 vs. ExPASy TrEMBL
Match: A0A6J1H7Q4 (metalloendoproteinase 1-like OS=Cucurbita moschata OX=3662 GN=LOC111460843 PE=3 SV=1)

HSP 1 Score: 298.1 bits (762), Expect = 4.1e-77
Identity = 157/296 (53.04%), Postives = 192/296 (64.86%), Query Frame = 0


Query: 13  ISFILLAAIAAHVTSARVLHNHAHNKSSHIIFSQNLLGSRKGHNIQGIHHVKTYLKRYGY 72
           + FIL+AAIA H   AR LH H   K S  IF Q+L+GS KGHN++GIH+V+ +L+RYGY
Sbjct: 9   LMFILVAAIAPHAIMARHLHTH---KFSSSIFPQHLVGSGKGHNMEGIHNVRAFLQRYGY 68

Query: 73  FT---HTQSNAFDDHLESALKTYQKFFNLNVSGILDNETLSLMSQPRCAVPDIFHNETST 132
            +    T+ NAFDD+LE ALK+YQKFFNLNVSGILD ETL LMSQPRC VPD+F  +   
Sbjct: 69  LSTNVDTKGNAFDDNLEFALKSYQKFFNLNVSGILDVETLELMSQPRCGVPDVFRID--- 128

Query: 133 RKGWGHEEHLHMGSHYLFFPRT-PKWPESKCRLRYSYYHDFPMAFKDPVNNALELWSAYL 192
                          Y FF  + PKW  SK  L+Y++  +FP  +K  V  A   WS+  
Sbjct: 129 ---------------YTFFRESKPKWRASKYNLKYAFVGNFPEQYKATVREATNTWSSVT 188

Query: 193 QFGFVEVGEDDYWADLKISFEMGEHGDGHPFIPTSGVLAHAFAPRDGRFHFNVEQCWTVG 252
            F F E   D+   D+KISFE+G+HGDG+PF+  SGVLAHAFAP DGR HFN    W  G
Sbjct: 189 GFTFTEGSVDN--TDVKISFEVGDHGDGYPFVEGSGVLAHAFAPEDGRLHFNTRHAWADG 248

Query: 253 ARPNCQDVMSVAVHEIGHLLGLGHSGAEEAIMWPCIRPNAVKGLFQDNVAGIKALY 305
           A P   DV +VA+HE+GH+LGLGHS    A+MWP   PN V GL  D+V GI ALY
Sbjct: 249 AYPYKYDVGTVALHELGHVLGLGHSAFRYAVMWPHTPPNFVTGLDNDDVNGIMALY 281

[Close]

BLAST of Moc04g35750 vs. TAIR 10
Match: AT1G70170.1 (matrix metalloproteinase )

HSP 1 Score: 188.3 bits (477), Expect = 8.9e-48
Identity = 109/281 (38.79%), Postives = 147/281 (52.31%), Query Frame = 0


Query: 47  NLLGSRKGHNIQGIHHVKTYLKRYGYFTHTQS----NAFDDHLESALKTYQKFFNLNVSG 106
           N  G   G N+ G++ +K Y +R+GY   T S    + FDD L++A++ YQ  FNLNV+G
Sbjct: 46  NFTGCHHGQNVDGLYRIKKYFQRFGYIPETFSGNFTDDFDDILKAAVELYQTNFNLNVTG 105

Query: 107 ILDNETLSLMSQPRCAVPDIFHNETSTRKGWGHE--------EHLHMGSHYLFFPRTPKW 166
            LD  T+  +  PRC  PD+  N TS   G   +         HLH    Y  FP  P+W
Sbjct: 106 ELDALTIQHIVIPRCGNPDVV-NGTSLMHGGRRKTFEVNFSRTHLHAVKRYTLFPGEPRW 165

Query: 167 PESKCRLRYSYYHDFPMA--FKDPVNNALELWSAYLQFGFVEVGEDDYWADLKISFEMGE 226
           P ++  L Y++    P+    K   + A   WS      F  + E    +D+ I F  G+
Sbjct: 166 PRNRRDLTYAFDPKNPLTEEVKSVFSRAFGRWSDVTALNFT-LSESFSTSDITIGFYTGD 225

Query: 227 HGDGHPFIPTSGVLAHAFAPRDGRFHFNVEQCWTVGARPN-------CQDVMSVAVHEIG 286
           HGDG PF    G LAHAF+P  G+FH + ++ W V    +         D+ SVAVHEIG
Sbjct: 226 HGDGEPFDGVLGTLAHAFSPPSGKFHLDADENWVVSGDLDSFLSVTAAVDLESVAVHEIG 285

Query: 287 HLLGLGHSGAEEAIMWPCIRPNAVK-GLFQDNVAGIKALYG 306
           HLLGLGHS  EE+IM+P I     K  L  D+V GI+ LYG
Sbjct: 286 HLLGLGHSSVEESIMYPTITTGKRKVDLTNDDVEGIQYLYG 324

[Close]

BLAST of Moc04g35750 vs. TAIR 10
Match: AT4G16640.1 (Matrixin family protein )

HSP 1 Score: 184.5 bits (467), Expect = 1.3e-46
Identity = 110/285 (38.60%), Postives = 145/285 (50.88%), Query Frame = 0


Query: 34  HAHNKSSHIIFSQNLLGSRKGHNIQGIHHVKTYLKRYGYF---THTQSNAFDDHLESALK 93
           H  N + H      L+  + G ++ G+  +K YL R+GY    +   S+ FD  LESA+ 
Sbjct: 46  HVSNSTWHDF--SRLVDVQIGSHVSGVSELKRYLHRFGYVNDGSEIFSDVFDGPLESAIS 105

Query: 94  TYQKFFNLNVSGILDNETLSLMSQPRCAVPDIFHNETSTRKGWGHEEHLHMGSHYLFFPR 153
            YQ+   L ++G LD  T++LMS PRC V D             + + LH  +HY +F  
Sbjct: 106 LYQENLGLPITGRLDTSTVTLMSLPRCGVSDTHMTI--------NNDFLHTTAHYTYFNG 165

Query: 154 TPKWPESKCRLRYSYYHDFPMAFKDPV----NNALELWSAYLQFGFVEVGEDDYWADLKI 213
            PKW         S  H       + V      A   WS+ +   F EV +D   ADLKI
Sbjct: 166 KPKWNRDTLTYAISKTHKLDYLTSEDVKTVFRRAFSQWSSVIPVSFEEV-DDFTTADLKI 225

Query: 214 SFEMGEHGDGHPFIPTSGVLAHAFAPRDGRFHFNVEQCWTV-----GARPNCQDVMSVAV 273
            F  G+HGDG PF    G LAHAFAP +GR H +  + W V     G+     D+ SVA 
Sbjct: 226 GFYAGDHGDGLPFDGVLGTLAHAFAPENGRLHLDAAETWIVDDDLKGSSEVAVDLESVAT 285

Query: 274 HEIGHLLGLGHSGAEEAIMWPCIRPNAVK-GLFQDNVAGIKALYG 306
           HEIGHLLGLGHS  E A+M+P +RP   K  L  D+VAG+  LYG
Sbjct: 286 HEIGHLLGLGHSSQESAVMYPSLRPRTKKVDLTVDDVAGVLKLYG 319

[Close]

BLAST of Moc04g35750 vs. TAIR 10
Match: AT1G24140.1 (Matrixin family protein )

HSP 1 Score: 181.8 bits (460), Expect = 8.3e-46
Identity = 104/283 (36.75%), Postives = 142/283 (50.18%), Query Frame = 0


Query: 47  NLLGSRKGHNIQGIHHVKTYLKRYGYFTHTQ-----SNAFDDHLESALKTYQKFFNLNVS 106
           N  G   G    G++ +K Y + +GY T T      ++ FDD L++A++ YQ+ F LNV+
Sbjct: 48  NFTGCHAGKKYDGLYMLKQYFQHFGYITETNLSGNFTDDFDDILKNAVEMYQRNFQLNVT 107

Query: 107 GILDNETLSLMSQPRCAVPDIFHNETSTRKG--------WGHEEHLHMGSHYLFFPRTPK 166
           G+LD  TL  +  PRC  PD+ +  ++   G         G  +  H   HY FFP  P+
Sbjct: 108 GVLDELTLKHVVIPRCGNPDVVNGTSTMHSGRKTFEVSFAGRGQRFHAVKHYSFFPGEPR 167

Query: 167 WPESKCRLRYSY--YHDFPMAFKDPVNNALELWSAYLQFGFVEVGEDDYWADLKISFEMG 226
           WP ++  L Y++   +      K   + A   W       F  V E    +D+ I F  G
Sbjct: 168 WPRNRRDLTYAFDPRNALTEEVKSVFSRAFTRWEEVTPLTFTRV-ERFSTSDISIGFYSG 227

Query: 227 EHGDGHPFIPTSGVLAHAFAPRDGRFHFNVEQCWTVGAR--------PNCQDVMSVAVHE 286
           EHGDG PF      LAHAF+P  G FH + E+ W V               D+ SVAVHE
Sbjct: 228 EHGDGEPFDGPMRTLAHAFSPPTGHFHLDGEENWIVSGEGGDGFISVSEAVDLESVAVHE 287

Query: 287 IGHLLGLGHSGAEEAIMWPCIRPNAVK-GLFQDNVAGIKALYG 306
           IGHLLGLGHS  E +IM+P IR    K  L  D+V G++ LYG
Sbjct: 288 IGHLLGLGHSSVEGSIMYPTIRTGRRKVDLTTDDVEGVQYLYG 329

[Close]

BLAST of Moc04g35750 vs. TAIR 10
Match: AT1G59970.1 (Matrixin family protein )

HSP 1 Score: 180.3 bits (456), Expect = 2.4e-45
Identity = 106/272 (38.97%), Postives = 142/272 (52.21%), Query Frame = 0


Query: 48  LLGSRKGHNIQGIHHVKTYLKRYGYFTHTQ--SNAFDDHLESALKTYQKFFNLNVSGILD 107
           L G   G NI G+  +K Y +R+GY T T   ++ FDD L+SA+ TYQK FNL V+G LD
Sbjct: 48  LAGCHIGENINGLSKLKQYFRRFGYITTTGNCTDDFDDVLQSAINTYQKNFNLKVTGKLD 107

Query: 108 NETLSLMSQPRCAVPDIFHNETSTRKGWGHEEHLHMGSHYLFFPRTPKWPESKCRLRYSY 167
           + TL  + +PRC  PD+    +    G    + L     Y FFP  P+WP+ K  L Y++
Sbjct: 108 SSTLRQIVKPRCGNPDLIDGVSEMNGG----KILRTTEKYSFFPGKPRWPKRKRDLTYAF 167

Query: 168 --YHDFPMAFKDPVNNALELWSAYLQFGFVEVGEDDYWADLKISFEMGEHGDGHPFIPTS 227
              ++     K   + A   W+      F    E    AD+ I F  GEHGDG PF    
Sbjct: 168 APQNNLTDEVKRVFSRAFTRWAEVTPLNFTR-SESILRADIVIGFFSGEHGDGEPFDGAM 227

Query: 228 GVLAHAFAPRDGRFHFNVEQCWTVG---------ARPNCQDVMSVAVHEIGHLLGLGHSG 287
           G LAHA +P  G  H + ++ W +                D+ SVAVHEIGHLLGLGHS 
Sbjct: 228 GTLAHASSPPTGMLHLDGDEDWLISNGEISRRILPVTTVVDLESVAVHEIGHLLGLGHSS 287

Query: 288 AEEAIMWPCIRPNAVK-GLFQDNVAGIKALYG 306
            E+AIM+P I     K  L +D++ GI+ LYG
Sbjct: 288 VEDAIMFPAISGGDRKVELAKDDIEGIQHLYG 314

[Close]

BLAST of Moc04g35750 vs. TAIR 10
Match: AT2G45040.1 (Matrixin family protein )

HSP 1 Score: 167.2 bits (422), Expect = 2.1e-41
Identity = 93/258 (36.05%), Postives = 137/258 (53.10%), Query Frame = 0


Query: 60  IHHVKTYLKRYGYFTHTQSNAFDDHLESALKTYQKFFNLNVSGILDNETLSLMSQPRCAV 119
           I  +K +L++YGY    + +  D   E AL  YQK   L ++G  D++TLS +  PRC  
Sbjct: 50  IPEIKRHLQQYGYLPQNKESD-DVSFEQALVRYQKNLGLPITGKPDSDTLSQILLPRCGF 109

Query: 120 PDIFHNETSTRKGWGHEEHLHMGSHYLFFPRTPKWP-ESKCRLRYSYYHD------FPMA 179
           PD    +T+           H G  Y++FP  P+W  +   +L Y++  +       P  
Sbjct: 110 PDDVEPKTAP---------FHTGKKYVYFPGRPRWTRDVPLKLTYAFSQENLTPYLAPTD 169

Query: 180 FKDPVNNALELWSAYLQFGFVEVGEDDYWADLKISFEMGEHGDGHPFIPTSGVLAHAFAP 239
            +     A   W++ +   F+E  ED   AD+KI F  G+HGDG PF    GVLAH F+P
Sbjct: 170 IRRVFRRAFGKWASVIPVSFIET-EDYVIADIKIGFFNGDHGDGEPFDGVLGVLAHTFSP 229

Query: 240 RDGRFHFNVEQCWTVGARPN----CQDVMSVAVHEIGHLLGLGHSGAEEAIMWPCIRPNA 299
            +GR H +  + W V           D+ SVAVHEIGH+LGLGHS  ++A M+P ++P +
Sbjct: 230 ENGRLHLDKAETWAVDFDEEKSSVAVDLESVAVHEIGHVLGLGHSSVKDAAMYPTLKPRS 289

Query: 300 VK-GLFQDNVAGIKALYG 306
            K  L  D+V G+++LYG
Sbjct: 290 KKVNLNMDDVVGVQSLYG 296

The following BLAST results are available for this feature:

BLAST of Moc04g35750 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022133610.1	4.7e-184	100.00	metalloendoproteinase 1-like [Momordica charantia]	[more]
XP_022133608.1	3.8e-101	63.43	matrix metalloproteinase-9-like [Momordica charantia]	[more]
XP_023544311.1	4.2e-84	57.09	metalloendoproteinase 1-like [Cucurbita pepo subsp. pepo]	[more]
XP_023544665.1	6.8e-82	55.41	metalloendoproteinase 1-like [Cucurbita pepo subsp. pepo]	[more]
KAG6602464.1	1.5e-81	55.74	hypothetical protein SDJN03_07697, partial [Cucurbita argyrosperma subsp. sorori...	[more]

BLAST of Moc04g35750 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
O04529	1.3e-46	38.79	Metalloendoproteinase 2-MMP OS=Arabidopsis thaliana OX=3702 GN=2MMP PE=1 SV=1	[more]
P29136	1.1e-45	40.15	Metalloendoproteinase 1 OS=Glycine max OX=3847 PE=1 SV=2	[more]
O23507	1.8e-45	38.60	Metalloendoproteinase 1-MMP OS=Arabidopsis thaliana OX=3702 GN=1MMP PE=1 SV=1	[more]
Q5XF51	1.2e-44	36.75	Metalloendoproteinase 3-MMP OS=Arabidopsis thaliana OX=3702 GN=3MMP PE=1 SV=1	[more]
Q9ZUJ5	3.4e-44	38.97	Metalloendoproteinase 5-MMP OS=Arabidopsis thaliana OX=3702 GN=5MMP PE=1 SV=1	[more]

BLAST of Moc04g35750 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1BVR3	2.3e-184	100.00	metalloendoproteinase 1-like OS=Momordica charantia OX=3673 GN=LOC111006149 PE=3...	[more]
A0A6J1BZL4	1.8e-101	63.43	matrix metalloproteinase-9-like OS=Momordica charantia OX=3673 GN=LOC111006147 P...	[more]
A0A6J1H7I1	4.0e-80	54.39	metalloendoproteinase 1-like OS=Cucurbita moschata OX=3662 GN=LOC111460787 PE=3 ...	[more]
A0A6J1H5Y2	2.9e-78	55.12	metalloendoproteinase 1-like OS=Cucurbita moschata OX=3662 GN=LOC111460835 PE=3 ...	[more]
A0A6J1H7Q4	4.1e-77	53.04	metalloendoproteinase 1-like OS=Cucurbita moschata OX=3662 GN=LOC111460843 PE=3 ...	[more]

BLAST of Moc04g35750 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G70170.1	8.9e-48	38.79	matrix metalloproteinase	[more]
AT4G16640.1	1.3e-46	38.60	Matrixin family protein	[more]
AT1G24140.1	8.3e-46	36.75	Matrixin family protein	[more]
AT1G59970.1	2.4e-45	38.97	Matrixin family protein	[more]
AT2G45040.1	2.1e-41	36.05	Matrixin family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (OHB3-1) v2
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR021190	Peptidase M10A	PRINTS	PR00138	MATRIXIN	coord: 112..125 score: 49.13 coord: 292..305 score: 53.31 coord: 259..284 score: 56.61 coord: 177..192 score: 26.68 coord: 202..230 score: 52.52
IPR021190	Peptidase M10A	PIRSF	PIRSF001191	MMP_stromelysin	coord: 7..307 e-value: 5.9E-55 score: 184.7
IPR006026	Peptidase, metallopeptidase	SMART	SM00235	col_5	coord: 150..306 e-value: 4.2E-20 score: 82.8
IPR002477	Peptidoglycan binding-like	PFAM	PF01471	PG_binding_1	coord: 59..112 e-value: 2.4E-14 score: 53.2
IPR001818	Peptidase M10, metallopeptidase	PFAM	PF00413	Peptidase_M10	coord: 172..305 e-value: 7.2E-34 score: 116.9
IPR024079	Metallopeptidase, catalytic domain superfamily	GENE3D	3.40.390.10	Collagenase (Catalytic Domain)	coord: 49..307 e-value: 3.7E-65 score: 221.7
None	No IPR available	PANTHER	PTHR10201:SF282	METALLOENDOPROTEINASE 3-MMP-LIKE	coord: 34..304
None	No IPR available	PANTHER	PTHR10201	MATRIX METALLOPROTEINASE	coord: 34..304
None	No IPR available	SUPERFAMILY	55486	Metalloproteases ("zincins"), catalytic domain	coord: 147..306
IPR036365	PGBD-like superfamily	SUPERFAMILY	47090	PGBD-like	coord: 54..122

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Moc04g35750.1	Moc04g35750.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006508	proteolysis
cellular_component	GO:0031225	anchored component of membrane
cellular_component	GO:0031012	extracellular matrix
molecular_function	GO:0004222	metalloendopeptidase activity
molecular_function	GO:0008270	zinc ion binding
molecular_function	GO:0008237	metallopeptidase activity