Moc04g34620 (gene) Bitter gourd (OHB3-1) v2

Overview
NameMoc04g34620
Typegene
OrganismMomordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
DescriptionMethionine--tRNA ligase
Locationchr4: 26107954 .. 26113190 (+)
RNA-Seq ExpressionMoc04g34620
SyntenyMoc04g34620
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCGGCTAGAATAAACTCCTCCATACAAAGTTCTCTCTGGCTCTTAAACCCTTTGCAGAGTTCTCTCAATCTCAATCTCAATCTCAATCTCAATCTCAGAACCCGCCTCAATTTCTATCGAAATCTGTTGCCCTCCCCATCTTATTCTGCTTCTTCATCAAAACGAGCTATATTCTGTACCTATGCGAGCAATTGCAATTCCCATGAAAGCGGAGCCGCAACAGACCCCTTTGTCATCACAACACCACTTTATTATGTTAATGCTGCTCCTCACATGGGGAGTGCTTATACCACCATTGCCGCTGATGCCATTGCGCGGTTTCAGGTTTTTTTTCTTCTTCTTAATCTATAATATTCATGCTGTTCGTTTAATTTTTGCTTTTACAAATTAGATGGGGCGTTTGTATATCGCTTGCTTGAATAAGCTCTGAGTAGTTTTTGTTCCTCTCATTTGTTGGTTATTTTTTTGTCGGTACTTGGTAGCTGAAATCGAATATATTGTCACCATAATTGACTTCTCTACCTGTTTTCTTTGTATGTTTCATTATATCACAGCCTGTATTTGAGAGCAAGAACGTTGAGAGTTTCAATTCTCACACCTCTCTCTTTCTGATATCTGCTGCTTACGATTGTTAATAACTGAAATTTTGGAATACAGTTTTACCATTAATCGCTCCTAACTGAATTAAAAAAGTTAAGTCCCTACCTTGCCGCATGGGAGTGTGTAGCAAGAATTAATTTACTTAGGGTCTAGCTGAAATTTGATTGTGTAGCAAGATGCTTTTAACAATTTGTTTTATAGTTGGATTTGTTTAGAAGAACTGAACTTTAAGTTTACAAATTAAACAAATATATCATTTGTGCAAATTAGAACCAATGCTCCTCAGTTACTGTTGCAGTCTTCCTCTAAGGGATATTATAAATGGTCAATAAGTTTTGGTATGTCCTATAGAGTTGATTTCTCGTATAGCTTACTAGTTGGCAAATTCCGGTGTTTGATTAGAACTATTTCACCATGCTTATAGCCAGTTTCCTCGGAATATTATGCTAATTTTTGGCTAACCTCTGGTGTCAAATATAATTATAGAGGCTGCTAGGGAAGAAAGTTATATTCATTACTGGGACAGATGAGCATGGGGAGAAGATTGCCACAGCTGCTGCTGCTCAAGGTTCTGCCCCAAGTGAACATTGTGATGCCATTTCAGAGGCATACAAGACACTCTGGAAAGATGTAGTCCAAACACATCACAGGATTTTCTTCTCAGTTTGCTGTAATTCATTTATTAGATATTTGAAATTATTCATAGTATTATTACATTCTTATTCTTTTTCTTCCTTTTTTCCCCTTTGCAGCTAGATGTAGCTTATGACAAGTTCATTCGAACAACTGATCCTAAACATGAGGCAATTGTAAAGGAATTTTATTCCAAGGTTCTTGCCAATGGCGATATTTATAGAGCTGACTATGAGGGACTTTATTGTGTCAATTGTGAAGAATACAAGGTGTTCTTTTTACCTTTTTTTTTTTTTAACTTTTTGTCTTCCTCCCTTTACCCATTTGAAAATTTTGGATTTGGATTCATATGATAATTGTGCGCCAAATTTCGTTTTATTGTTAACATTGCTTCTTTCCTCAATTATGATAATATATTGACCTAACTCTGCTTTTCCAATAACTATTTTCCTGGGGTATGGATGCATTTAACAGTAGTTATTTCTACAATTTTGAAGGTATAGTATGCACGAATTCTCTTGAGTTTTGTAAGAGCTTCCATTTATAAACCTGCAGGATGAAAAAGAACTCCTTGCCAACAATTGTTGTCCTATACACCTGAAGCCCTGTGCTTCAAGGAAAGAAGACAATTACTTTTTTGCATTGTCAAAATATCAAGGGTTGCTTGAAGAAACTTTAACACAGAATCCAGGTTTTGTGCAGCCCGCCTTCCGTTTGAATGAGGTGACTATAGGGGATTTTCTACTTGTGTGTGTGTTTATGTGTGAAATTTGTTACTTCTCTCCTAAAGTACTGCATTACATAATTTTCTTTGAAAAGTTCCGGATCAAATATATTTGTGTCGGAACTGATGTTAAATCCAAGAGAAACAAATGGCTTGGAGCGTACAAAAAGGCGTTCAAATCAGCTGAACTTTAAAACAATGGGAAGTAAAAAAAAAACTATTAGTAAGAAGCCCCCAAAGGAAACCTGAGAAACAGTGTGATTCCAAACCCTAATTCTGATAGAACTTTTATTGTGATACCAATAAACAAGAAGTTGATTGACTTATTTAATGATCAATTGTAGTTGTAGATTGACTTGCTAAGAGTGATAATATAGAACTCCATGCATATATACCATAAATTATTATTATTCAAGCCTTCAAGGTCTACCATTTAGTATGACCTAAACTAAATGTAATTCTTCTAATTGCTCATCAAATCTCTTTGATCTAATTTGATATTGTATTTATATACGAATTTTTTCTTACAACAGAATGTCAGTTCTATTATTTGTTACAATTTTTTTCCACCTTGCCGATCGTGAATCATCCTTTGATTAATTCAGCACCATATCTTCCAAGGTGCAAAGTTGGATTAGAAGTGGCCTGAAAGACTTCTCTATCTCCAGAGCATCAGTTGAATGGGGAATTCCAGTTCCCAATGATCAGAAGCAAACGATTTATGTTTGGTTTGATGCATTATTAGGGTAATATCTTCCCTATGTTTTTGTAATGTCTTATATATCGTGACTTCTTGAGCCTTTATGATTATGGCCAACTTTTTTTATTTATTTACTTCTTAGCTCTTCATCACAATCCAGTACAAACATACGGTTTATGTAAATTTGCAACAACTATGCACCATTTTGCTATAGTCTGTCTTTAATCTTAACTATATGAGAATTCCATAATGTAGTTATATATCAGCACTATCAGAGAATGGTCAGCAACAGAATTTACAAAGGGCCGTGGCTTCGGGTTGGCCTGCCTCTTTACACCTGATTGGGAAGGTAAGAGACACATTCCTGTTCTTTGTACGTAGACTTATCTGGCCCTAGCTGAAAACTTCTCTCTCTCTCTCTCTCTCTCCATCTGTTTTTCAGATTATGTCTTTTCTACTATATTTCTCTTGAATATAGCAAGAAGTGAAATTATGAATCAACTCTAGTTTCTGTGTTATTATTGTATTCTGGAGGTTCTGATGATTGTTTCCCTTTTCTTTTCTTTACTGAGTGCAAGCTCAGTCTGTAGGATGCCGATCTGATGTTTTTGTCATTCTTTTTTGTAGGATATTTTACGTTTCCATGCAGTTTATTGGCCTGCTATGCTCATGTCTGCTGGACTAAAACTCCCAAAGATGGTTTTCGGCCATGGGTTCTTGACAAAGGTATTATGTTTGGCCTCCTAAATTTACTACAAAGGAATTTAGTTGGCCATGATATGTCGAACTCTAATTGATGCAGAAGAGAATTAATATTTGTCAAATTGACGAAGTCTATAAAGAATCAAATTAACTTGTCACCATTGCTATAGTATATAATGTTAATATTATTGCATCTTGTGAATTATAAAGGGTCTTGATTGTCTTGTGAATCTTATTGAATCTCTTGGAGTTGCAACATTCAATGCCCCTCCTAACCAAACACTTCCAACAATTGGATTCCTTGACTTTTAGCTTCATGTTCACAAAGAAGCTTGAACCAGTGAATCTCTGCAGAGGCACAAATACTTCGTTATTATTTCATGCAGCAACTCTTTTTGCTTTAACATTATAGGATGGGATGAAGATGGGCAAGTCATTAGGCAATACTCTCGAACCAAATGAATTGGTTCAAAAGTTTGGCTCTGATGCAGTGAGATACTTCTTCCTTCAAGAGGTGGAATTCGGTAGTGATGGGGACTATGCTGAGGATCGTTTCATCAATATTGTCAATGCACATCTTGCAAATACAATAGGTATTTATAAACCGTAATTCCTTCCGTTTGGAAAATTACAGCACTTTTGCTGTACACTTCTGTTTTTTCTTTTTTAATAATCGGGAGTCGAGCCTCCCTAAACTTGGGGGCACCTGTGGGTTGTGCCCACTCCAAGGACCATATTTGGGAGACATCAAGAGTTTTTGGTATTAAGCTCACATTTTCTAGGCCGGTATGATCCAGAGATCCAAAGCTTTTGCCAACGAGGACCACCACTTGGGGGCTTGAGGGAGATTTCTGTTTGACACAACATAATTCTCGATGTTTCTTCTGCAGGAAATCTGCTGAACCGAACTCTCGGACTCCTTAAGAAGAACTGCCAATCAACTTTGGTGGTTGATTCTACTGCTGCAGCTGAAGGAAATTCATTTAAGGACAAAGTTGAAGAGCTGGTAACAGTTTGCTCTGAATTTACGTTGTTGTTTGTTATTTGAGATGGCGTGGAATTAGTAGACCCTGAAATCTTCTCTTGAATCTCTATTGTGAAAGGCACTGAATCCATCGTGGTGCTGATATTGTCTGTGTGTCCCTCCATTTATGACTTCGTGATTGACATGTTCATGAATTTTTGCAAACTATGTTGATTTTATTTGTGTGCCTGGTAGGTTCTGAAAGCTCGAAATCATTACGAAGGACTCTCCCTCTCATCGGCTTGTGAGGCTGTGTTGGAGATCGGCAATGCTGGAAACTCTTACATGGATGAGCAAGCGCCATGGTCTCTATTCAAGCAAGGTGGTGCCTCTTCAGACGCTGCTGCAAAGGTTGTTCAGTTCAAGAACCCATCTTTCTTCACGATCATGATCTCGGATGCGAATTCTCGATCAACTTTTCCTTCCAACATACAAGAAACCATTCTCTGACAACCATCTGCCTTGGTTTTCTCAACAGGACCTTGTACTTATACTTGAAGCAATGCGAATCATAGCCGTTGCACTATCCCCAGTGACTCCATGCTTAGGCTGGAGAATATACGCCCAACTCGGGTTCTCGAAGGACCAGTTTGAAGCTGCAACATGGGTCTGTTTCTCTTTTCACACTTGTGTGTTTGATATCAGTTCTTTTTATTACTAGTGACTGATAATGTGGTTTTTGGATGTAGAATGAGACAAAGTGGGGAGGGCTGAAGGCTGGCCAGGTCATGGCTCCTCCAAATCCAGTTTTTGCTAGGATTGAACAATCCACTGGTAGTGATGAAGATGAAGTTCCTAAAAAACCAGTGAAAAGTAAGAAGAAAATAGCACAAGGGGTGGCAGAAGCCTAA

mRNA sequence

ATGGCGGCTAGAATAAACTCCTCCATACAAAGTTCTCTCTGGCTCTTAAACCCTTTGCAGAGTTCTCTCAATCTCAATCTCAATCTCAATCTCAATCTCAGAACCCGCCTCAATTTCTATCGAAATCTGTTGCCCTCCCCATCTTATTCTGCTTCTTCATCAAAACGAGCTATATTCTGTACCTATGCGAGCAATTGCAATTCCCATGAAAGCGGAGCCGCAACAGACCCCTTTGTCATCACAACACCACTTTATTATGTTAATGCTGCTCCTCACATGGGGAGTGCTTATACCACCATTGCCGCTGATGCCATTGCGCGGTTTCAGAGGCTGCTAGGGAAGAAAGTTATATTCATTACTGGGACAGATGAGCATGGGGAGAAGATTGCCACAGCTGCTGCTGCTCAAGGTTCTGCCCCAAGTGAACATTGTGATGCCATTTCAGAGGCATACAAGACACTCTGGAAAGATCTAGATGTAGCTTATGACAAGTTCATTCGAACAACTGATCCTAAACATGAGGCAATTGTAAAGGAATTTTATTCCAAGGTTCTTGCCAATGGCGATATTTATAGAGCTGACTATGAGGGACTTTATTGTGTCAATTGTGAAGAATACAAGGATGAAAAAGAACTCCTTGCCAACAATTGTTGTCCTATACACCTGAAGCCCTGTGCTTCAAGGAAAGAAGACAATTACTTTTTTGCATTGTCAAAATATCAAGGGTTGCTTGAAGAAACTTTAACACAGAATCCAGGTTTTGTGCAGCCCGCCTTCCGTTTGAATGAGGTGCAAAGTTGGATTAGAAGTGGCCTGAAAGACTTCTCTATCTCCAGAGCATCAGTTGAATGGGGAATTCCAGTTCCCAATGATCAGAAGCAAACGATTTATGTTTGGTTTGATGCATTATTAGGTTATATATCAGCACTATCAGAGAATGGTCAGCAACAGAATTTACAAAGGGCCGTGGCTTCGGGTTGGCCTGCCTCTTTACACCTGATTGGGAAGGATATTTTACGTTTCCATGCAGTTTATTGGCCTGCTATGCTCATGTCTGCTGGACTAAAACTCCCAAAGATGGTTTTCGGCCATGGGTTCTTGACAAAGGATGGGATGAAGATGGGCAAGTCATTAGGCAATACTCTCGAACCAAATGAATTGGTTCAAAAGTTTGGCTCTGATGCAGTGAGATACTTCTTCCTTCAAGAGGTGGAATTCGGTAGTGATGGGGACTATGCTGAGGATCGTTTCATCAATATTGTCAATGCACATCTTGCAAATACAATAGGAAATCTGCTGAACCGAACTCTCGGACTCCTTAAGAAGAACTGCCAATCAACTTTGGTGGTTGATTCTACTGCTGCAGCTGAAGGAAATTCATTTAAGGACAAAGTTGAAGAGCTGGTTCTGAAAGCTCGAAATCATTACGAAGGACTCTCCCTCTCATCGGCTTGTGAGGCTGTGTTGGAGATCGGCAATGCTGGAAACTCTTACATGGATGAGCAAGCGCCATGGTCTCTATTCAAGCAAGGTGGTGCCTCTTCAGACGCTGCTGCAAAGGACCTTGTACTTATACTTGAAGCAATGCGAATCATAGCCGTTGCACTATCCCCAGTGACTCCATGCTTAGGCTGGAGAATATACGCCCAACTCGGGTTCTCGAAGGACCAGTTTGAAGCTGCAACATGGAATGAGACAAAGTGGGGAGGGCTGAAGGCTGGCCAGGTCATGGCTCCTCCAAATCCAGTTTTTGCTAGGATTGAACAATCCACTGGTAGTGATGAAGATGAAGTTCCTAAAAAACCAGTGAAAAGTAAGAAGAAAATAGCACAAGGGGTGGCAGAAGCCTAA

Coding sequence (CDS)

ATGGCGGCTAGAATAAACTCCTCCATACAAAGTTCTCTCTGGCTCTTAAACCCTTTGCAGAGTTCTCTCAATCTCAATCTCAATCTCAATCTCAATCTCAGAACCCGCCTCAATTTCTATCGAAATCTGTTGCCCTCCCCATCTTATTCTGCTTCTTCATCAAAACGAGCTATATTCTGTACCTATGCGAGCAATTGCAATTCCCATGAAAGCGGAGCCGCAACAGACCCCTTTGTCATCACAACACCACTTTATTATGTTAATGCTGCTCCTCACATGGGGAGTGCTTATACCACCATTGCCGCTGATGCCATTGCGCGGTTTCAGAGGCTGCTAGGGAAGAAAGTTATATTCATTACTGGGACAGATGAGCATGGGGAGAAGATTGCCACAGCTGCTGCTGCTCAAGGTTCTGCCCCAAGTGAACATTGTGATGCCATTTCAGAGGCATACAAGACACTCTGGAAAGATCTAGATGTAGCTTATGACAAGTTCATTCGAACAACTGATCCTAAACATGAGGCAATTGTAAAGGAATTTTATTCCAAGGTTCTTGCCAATGGCGATATTTATAGAGCTGACTATGAGGGACTTTATTGTGTCAATTGTGAAGAATACAAGGATGAAAAAGAACTCCTTGCCAACAATTGTTGTCCTATACACCTGAAGCCCTGTGCTTCAAGGAAAGAAGACAATTACTTTTTTGCATTGTCAAAATATCAAGGGTTGCTTGAAGAAACTTTAACACAGAATCCAGGTTTTGTGCAGCCCGCCTTCCGTTTGAATGAGGTGCAAAGTTGGATTAGAAGTGGCCTGAAAGACTTCTCTATCTCCAGAGCATCAGTTGAATGGGGAATTCCAGTTCCCAATGATCAGAAGCAAACGATTTATGTTTGGTTTGATGCATTATTAGGTTATATATCAGCACTATCAGAGAATGGTCAGCAACAGAATTTACAAAGGGCCGTGGCTTCGGGTTGGCCTGCCTCTTTACACCTGATTGGGAAGGATATTTTACGTTTCCATGCAGTTTATTGGCCTGCTATGCTCATGTCTGCTGGACTAAAACTCCCAAAGATGGTTTTCGGCCATGGGTTCTTGACAAAGGATGGGATGAAGATGGGCAAGTCATTAGGCAATACTCTCGAACCAAATGAATTGGTTCAAAAGTTTGGCTCTGATGCAGTGAGATACTTCTTCCTTCAAGAGGTGGAATTCGGTAGTGATGGGGACTATGCTGAGGATCGTTTCATCAATATTGTCAATGCACATCTTGCAAATACAATAGGAAATCTGCTGAACCGAACTCTCGGACTCCTTAAGAAGAACTGCCAATCAACTTTGGTGGTTGATTCTACTGCTGCAGCTGAAGGAAATTCATTTAAGGACAAAGTTGAAGAGCTGGTTCTGAAAGCTCGAAATCATTACGAAGGACTCTCCCTCTCATCGGCTTGTGAGGCTGTGTTGGAGATCGGCAATGCTGGAAACTCTTACATGGATGAGCAAGCGCCATGGTCTCTATTCAAGCAAGGTGGTGCCTCTTCAGACGCTGCTGCAAAGGACCTTGTACTTATACTTGAAGCAATGCGAATCATAGCCGTTGCACTATCCCCAGTGACTCCATGCTTAGGCTGGAGAATATACGCCCAACTCGGGTTCTCGAAGGACCAGTTTGAAGCTGCAACATGGAATGAGACAAAGTGGGGAGGGCTGAAGGCTGGCCAGGTCATGGCTCCTCCAAATCCAGTTTTTGCTAGGATTGAACAATCCACTGGTAGTGATGAAGATGAAGTTCCTAAAAAACCAGTGAAAAGTAAGAAGAAAATAGCACAAGGGGTGGCAGAAGCCTAA

Protein sequence

MAARINSSIQSSLWLLNPLQSSLNLNLNLNLNLRTRLNFYRNLLPSPSYSASSSKRAIFCTYASNCNSHESGAATDPFVITTPLYYVNAAPHMGSAYTTIAADAIARFQRLLGKKVIFITGTDEHGEKIATAAAAQGSAPSEHCDAISEAYKTLWKDLDVAYDKFIRTTDPKHEAIVKEFYSKVLANGDIYRADYEGLYCVNCEEYKDEKELLANNCCPIHLKPCASRKEDNYFFALSKYQGLLEETLTQNPGFVQPAFRLNEVQSWIRSGLKDFSISRASVEWGIPVPNDQKQTIYVWFDALLGYISALSENGQQQNLQRAVASGWPASLHLIGKDILRFHAVYWPAMLMSAGLKLPKMVFGHGFLTKDGMKMGKSLGNTLEPNELVQKFGSDAVRYFFLQEVEFGSDGDYAEDRFINIVNAHLANTIGNLLNRTLGLLKKNCQSTLVVDSTAAAEGNSFKDKVEELVLKARNHYEGLSLSSACEAVLEIGNAGNSYMDEQAPWSLFKQGGASSDAAAKDLVLILEAMRIIAVALSPVTPCLGWRIYAQLGFSKDQFEAATWNETKWGGLKAGQVMAPPNPVFARIEQSTGSDEDEVPKKPVKSKKKIAQGVAEA
Homology
BLAST of Moc04g34620 vs. NCBI nr
Match: XP_022133974.1 (methionine--tRNA ligase, chloroplastic/mitochondrial [Momordica charantia])

HSP 1 Score: 1232.2 bits (3187), Expect = 0.0e+00
Identity = 616/616 (100.00%), Postives = 616/616 (100.00%), Query Frame = 0

Query: 1   MAARINSSIQSSLWLLNPLQSSLNLNLNLNLNLRTRLNFYRNLLPSPSYSASSSKRAIFC 60
           MAARINSSIQSSLWLLNPLQSSLNLNLNLNLNLRTRLNFYRNLLPSPSYSASSSKRAIFC
Sbjct: 1   MAARINSSIQSSLWLLNPLQSSLNLNLNLNLNLRTRLNFYRNLLPSPSYSASSSKRAIFC 60

Query: 61  TYASNCNSHESGAATDPFVITTPLYYVNAAPHMGSAYTTIAADAIARFQRLLGKKVIFIT 120
           TYASNCNSHESGAATDPFVITTPLYYVNAAPHMGSAYTTIAADAIARFQRLLGKKVIFIT
Sbjct: 61  TYASNCNSHESGAATDPFVITTPLYYVNAAPHMGSAYTTIAADAIARFQRLLGKKVIFIT 120

Query: 121 GTDEHGEKIATAAAAQGSAPSEHCDAISEAYKTLWKDLDVAYDKFIRTTDPKHEAIVKEF 180
           GTDEHGEKIATAAAAQGSAPSEHCDAISEAYKTLWKDLDVAYDKFIRTTDPKHEAIVKEF
Sbjct: 121 GTDEHGEKIATAAAAQGSAPSEHCDAISEAYKTLWKDLDVAYDKFIRTTDPKHEAIVKEF 180

Query: 181 YSKVLANGDIYRADYEGLYCVNCEEYKDEKELLANNCCPIHLKPCASRKEDNYFFALSKY 240
           YSKVLANGDIYRADYEGLYCVNCEEYKDEKELLANNCCPIHLKPCASRKEDNYFFALSKY
Sbjct: 181 YSKVLANGDIYRADYEGLYCVNCEEYKDEKELLANNCCPIHLKPCASRKEDNYFFALSKY 240

Query: 241 QGLLEETLTQNPGFVQPAFRLNEVQSWIRSGLKDFSISRASVEWGIPVPNDQKQTIYVWF 300
           QGLLEETLTQNPGFVQPAFRLNEVQSWIRSGLKDFSISRASVEWGIPVPNDQKQTIYVWF
Sbjct: 241 QGLLEETLTQNPGFVQPAFRLNEVQSWIRSGLKDFSISRASVEWGIPVPNDQKQTIYVWF 300

Query: 301 DALLGYISALSENGQQQNLQRAVASGWPASLHLIGKDILRFHAVYWPAMLMSAGLKLPKM 360
           DALLGYISALSENGQQQNLQRAVASGWPASLHLIGKDILRFHAVYWPAMLMSAGLKLPKM
Sbjct: 301 DALLGYISALSENGQQQNLQRAVASGWPASLHLIGKDILRFHAVYWPAMLMSAGLKLPKM 360

Query: 361 VFGHGFLTKDGMKMGKSLGNTLEPNELVQKFGSDAVRYFFLQEVEFGSDGDYAEDRFINI 420
           VFGHGFLTKDGMKMGKSLGNTLEPNELVQKFGSDAVRYFFLQEVEFGSDGDYAEDRFINI
Sbjct: 361 VFGHGFLTKDGMKMGKSLGNTLEPNELVQKFGSDAVRYFFLQEVEFGSDGDYAEDRFINI 420

Query: 421 VNAHLANTIGNLLNRTLGLLKKNCQSTLVVDSTAAAEGNSFKDKVEELVLKARNHYEGLS 480
           VNAHLANTIGNLLNRTLGLLKKNCQSTLVVDSTAAAEGNSFKDKVEELVLKARNHYEGLS
Sbjct: 421 VNAHLANTIGNLLNRTLGLLKKNCQSTLVVDSTAAAEGNSFKDKVEELVLKARNHYEGLS 480

Query: 481 LSSACEAVLEIGNAGNSYMDEQAPWSLFKQGGASSDAAAKDLVLILEAMRIIAVALSPVT 540
           LSSACEAVLEIGNAGNSYMDEQAPWSLFKQGGASSDAAAKDLVLILEAMRIIAVALSPVT
Sbjct: 481 LSSACEAVLEIGNAGNSYMDEQAPWSLFKQGGASSDAAAKDLVLILEAMRIIAVALSPVT 540

Query: 541 PCLGWRIYAQLGFSKDQFEAATWNETKWGGLKAGQVMAPPNPVFARIEQSTGSDEDEVPK 600
           PCLGWRIYAQLGFSKDQFEAATWNETKWGGLKAGQVMAPPNPVFARIEQSTGSDEDEVPK
Sbjct: 541 PCLGWRIYAQLGFSKDQFEAATWNETKWGGLKAGQVMAPPNPVFARIEQSTGSDEDEVPK 600

Query: 601 KPVKSKKKIAQGVAEA 617
           KPVKSKKKIAQGVAEA
Sbjct: 601 KPVKSKKKIAQGVAEA 616

BLAST of Moc04g34620 vs. NCBI nr
Match: XP_038891037.1 (methionine--tRNA ligase, chloroplastic/mitochondrial [Benincasa hispida])

HSP 1 Score: 1112.8 bits (2877), Expect = 0.0e+00
Identity = 557/616 (90.42%), Postives = 589/616 (95.62%), Query Frame = 0

Query: 1   MAARINSSIQSSLWLLNPLQSSLNLNLNLNLNLRTRLNFYRNLLPSPSYSASSSKRAIFC 60
           MAARIN+SIQ+SLWLLNP QSSLNL      +LRTRL+FY+NLL  PS S+SSSKRAIFC
Sbjct: 1   MAARINTSIQTSLWLLNPCQSSLNLK---PTHLRTRLHFYQNLLSLPS-SSSSSKRAIFC 60

Query: 61  TYASNCNSHESGAATDPFVITTPLYYVNAAPHMGSAYTTIAADAIARFQRLLGKKVIFIT 120
           T A++ NSH++GAAT+PFVITTPLYYVNAAPHMGSAYTTIAADAIARFQRLLGKKVIFIT
Sbjct: 61  TAATSFNSHDNGAATNPFVITTPLYYVNAAPHMGSAYTTIAADAIARFQRLLGKKVIFIT 120

Query: 121 GTDEHGEKIATAAAAQGSAPSEHCDAISEAYKTLWKDLDVAYDKFIRTTDPKHEAIVKEF 180
           GTDEHGEKIATAAA QGS+PSEHCD ISEAYKTLWKDLDVAYD+FIRTTDPKHEAIVKEF
Sbjct: 121 GTDEHGEKIATAAATQGSSPSEHCDVISEAYKTLWKDLDVAYDRFIRTTDPKHEAIVKEF 180

Query: 181 YSKVLANGDIYRADYEGLYCVNCEEYKDEKELLANNCCPIHLKPCASRKEDNYFFALSKY 240
           YS+VLANGDIYRADYEGLYCVNCEEYKDEKELLA+NCCPIHLKPCASR+EDNYFFALSKY
Sbjct: 181 YSRVLANGDIYRADYEGLYCVNCEEYKDEKELLADNCCPIHLKPCASRREDNYFFALSKY 240

Query: 241 QGLLEETLTQNPGFVQPAFRLNEVQSWIRSGLKDFSISRASVEWGIPVPNDQKQTIYVWF 300
           Q LLEETLTQNPGFVQPAFRLNEVQSWIRSGLKDFSISRASVEWGIPVPND+KQTIYVWF
Sbjct: 241 QRLLEETLTQNPGFVQPAFRLNEVQSWIRSGLKDFSISRASVEWGIPVPNDEKQTIYVWF 300

Query: 301 DALLGYISALSENGQQQNLQRAVASGWPASLHLIGKDILRFHAVYWPAMLMSAGLKLPKM 360
           DALLGYISALSENG+Q NL+R VASGWPASLHLIGKDILRFHAVYWPAMLMSAGLKLPKM
Sbjct: 301 DALLGYISALSENGEQPNLERVVASGWPASLHLIGKDILRFHAVYWPAMLMSAGLKLPKM 360

Query: 361 VFGHGFLTKDGMKMGKSLGNTLEPNELVQKFGSDAVRYFFLQEVEFGSDGDYAEDRFINI 420
           VFGHGFLTKDGMKMGKSLGNTLEPNELV KFGSDAVRYFFL+EVEFGSDGDYAEDRFINI
Sbjct: 361 VFGHGFLTKDGMKMGKSLGNTLEPNELVGKFGSDAVRYFFLREVEFGSDGDYAEDRFINI 420

Query: 421 VNAHLANTIGNLLNRTLGLLKKNCQSTLVVDSTAAAEGNSFKDKVEELVLKARNHYEGLS 480
           VNAHLANTIGNLLNRTLGLL+KNCQSTLVVDSTAAA GN FKDKVEEL++KARNHYE L+
Sbjct: 421 VNAHLANTIGNLLNRTLGLLQKNCQSTLVVDSTAAAGGNVFKDKVEELIVKARNHYEELA 480

Query: 481 LSSACEAVLEIGNAGNSYMDEQAPWSLFKQGGASSDAAAKDLVLILEAMRIIAVALSPVT 540
           LSSACEAVLEIGNAGNSYMDE+APWSLFKQGGAS+DAAAKDLVLILEAMRIIA+ALSPVT
Sbjct: 481 LSSACEAVLEIGNAGNSYMDERAPWSLFKQGGASTDAAAKDLVLILEAMRIIAIALSPVT 540

Query: 541 PCLGWRIYAQLGFSKDQFEAATWNETKWGGLKAGQVMAPPNPVFARIEQSTGSDEDEVPK 600
           P L WRIYAQLG+S+DQF AATWNETKWGGLKAGQVMAPPNPVFARIEQ+  + E+EVPK
Sbjct: 541 PRLSWRIYAQLGYSEDQFNAATWNETKWGGLKAGQVMAPPNPVFARIEQAIVNGEEEVPK 600

Query: 601 KPVKSKKKIAQGVAEA 617
           KPVKSKKKIAQGVAEA
Sbjct: 601 KPVKSKKKIAQGVAEA 612

BLAST of Moc04g34620 vs. NCBI nr
Match: XP_004142742.1 (methionine--tRNA ligase, chloroplastic/mitochondrial [Cucumis sativus] >KGN51284.1 hypothetical protein Csa_008274 [Cucumis sativus])

HSP 1 Score: 1110.9 bits (2872), Expect = 0.0e+00
Identity = 557/616 (90.42%), Postives = 584/616 (94.81%), Query Frame = 0

Query: 1   MAARINSSIQSSLWLLNPLQSSLNLNLNLNLNLRTRLNFYRNLLPSPSYSASSSKRAIFC 60
           MAARIN+SIQ+SLWLLNP  SSLNL      + RTRL+F +NLL  PS+  SSSKRAIFC
Sbjct: 1   MAARINTSIQTSLWLLNPFHSSLNLK---PTHFRTRLHFCQNLLSFPSH--SSSKRAIFC 60

Query: 61  TYASNCNSHESGAATDPFVITTPLYYVNAAPHMGSAYTTIAADAIARFQRLLGKKVIFIT 120
           T  SN NSH+SGAATDPFVITTPLYYVNAAPHMGSAYTTIAADA+ARFQRLLGKKVIFIT
Sbjct: 61  TSTSNFNSHDSGAATDPFVITTPLYYVNAAPHMGSAYTTIAADAVARFQRLLGKKVIFIT 120

Query: 121 GTDEHGEKIATAAAAQGSAPSEHCDAISEAYKTLWKDLDVAYDKFIRTTDPKHEAIVKEF 180
           GTDEHGEKIATAAA QGS PSEHCD ISEAYKTLWKDLDVAYDKFIRTTDPKHEAIVKEF
Sbjct: 121 GTDEHGEKIATAAATQGSTPSEHCDIISEAYKTLWKDLDVAYDKFIRTTDPKHEAIVKEF 180

Query: 181 YSKVLANGDIYRADYEGLYCVNCEEYKDEKELLANNCCPIHLKPCASRKEDNYFFALSKY 240
           YSKVLANGDIYRADYEGLYCVNCEEYKDEKELLA+NCCPIHLKPCASRKEDNYFFALSKY
Sbjct: 181 YSKVLANGDIYRADYEGLYCVNCEEYKDEKELLADNCCPIHLKPCASRKEDNYFFALSKY 240

Query: 241 QGLLEETLTQNPGFVQPAFRLNEVQSWIRSGLKDFSISRASVEWGIPVPNDQKQTIYVWF 300
           Q LLEETLTQNPGFVQPAFRLNEVQSWIRSGLKDFSISRASVEWGIPVPND+KQTIYVWF
Sbjct: 241 QRLLEETLTQNPGFVQPAFRLNEVQSWIRSGLKDFSISRASVEWGIPVPNDEKQTIYVWF 300

Query: 301 DALLGYISALSENGQQQNLQRAVASGWPASLHLIGKDILRFHAVYWPAMLMSAGLKLPKM 360
           DALLGYISALSENG+Q NLQ  VASGWPASLHLIGKDILRFHAVYWPAMLMSAGLKLPKM
Sbjct: 301 DALLGYISALSENGEQPNLQEVVASGWPASLHLIGKDILRFHAVYWPAMLMSAGLKLPKM 360

Query: 361 VFGHGFLTKDGMKMGKSLGNTLEPNELVQKFGSDAVRYFFLQEVEFGSDGDYAEDRFINI 420
           VFGHGFLTKDGMKMGKSLGNTLEPNELV+KFGSDAVRYFFL+EVEFGSDGDYAEDRFINI
Sbjct: 361 VFGHGFLTKDGMKMGKSLGNTLEPNELVEKFGSDAVRYFFLREVEFGSDGDYAEDRFINI 420

Query: 421 VNAHLANTIGNLLNRTLGLLKKNCQSTLVVDSTAAAEGNSFKDKVEELVLKARNHYEGLS 480
           VNAHLANTIGNLLNRTLGLLKKNCQSTLVVDSTAAA+GN+FKDKVEELV+KARN+YE L+
Sbjct: 421 VNAHLANTIGNLLNRTLGLLKKNCQSTLVVDSTAAADGNAFKDKVEELVVKARNNYEELA 480

Query: 481 LSSACEAVLEIGNAGNSYMDEQAPWSLFKQGGASSDAAAKDLVLILEAMRIIAVALSPVT 540
           LSSACEAVLEIGNAGNSYMDE+APWSLFKQGGASS+AAAKDLVLILEAMRIIA+ALSPVT
Sbjct: 481 LSSACEAVLEIGNAGNSYMDERAPWSLFKQGGASSNAAAKDLVLILEAMRIIAIALSPVT 540

Query: 541 PCLGWRIYAQLGFSKDQFEAATWNETKWGGLKAGQVMAPPNPVFARIEQSTGSDEDEVPK 600
           P L WRIYAQLG+SKDQF+AATWNETKWGGLKAGQVMAPPNPVFARIEQ+  + E+E PK
Sbjct: 541 PRLSWRIYAQLGYSKDQFDAATWNETKWGGLKAGQVMAPPNPVFARIEQAIVNGEEEAPK 600

Query: 601 KPVKSKKKIAQGVAEA 617
           KPVKSKKKIAQ VAE+
Sbjct: 601 KPVKSKKKIAQAVAES 611

BLAST of Moc04g34620 vs. NCBI nr
Match: KAA0066020.1 (methionine--tRNA ligase [Cucumis melo var. makuwa] >TYK05823.1 methionine--tRNA ligase [Cucumis melo var. makuwa])

HSP 1 Score: 1108.6 bits (2866), Expect = 0.0e+00
Identity = 557/616 (90.42%), Postives = 584/616 (94.81%), Query Frame = 0

Query: 1   MAARINSSIQSSLWLLNPLQSSLNLNLNLNLNLRTRLNFYRNLLPSPSYSASSSKRAIFC 60
           MAARIN+SIQ+SL LLNP  +SLNL      + RTRL+FY+NLL   SY  SSSKRAIFC
Sbjct: 1   MAARINTSIQTSLSLLNPFHNSLNLK---PTHFRTRLHFYQNLLSFASY--SSSKRAIFC 60

Query: 61  TYASNCNSHESGAATDPFVITTPLYYVNAAPHMGSAYTTIAADAIARFQRLLGKKVIFIT 120
           T  SN NSH++GAATDPFVITTPLYYVNAAPHMGSAYTTIAADAIARFQRLLGKKVI IT
Sbjct: 61  TTTSNFNSHDTGAATDPFVITTPLYYVNAAPHMGSAYTTIAADAIARFQRLLGKKVILIT 120

Query: 121 GTDEHGEKIATAAAAQGSAPSEHCDAISEAYKTLWKDLDVAYDKFIRTTDPKHEAIVKEF 180
           GTDEHGEKIATAAA QGS PSEHCD ISEAYKTLWKDLDVAYDKFIRTTDPKHEAIVKEF
Sbjct: 121 GTDEHGEKIATAAATQGSTPSEHCDIISEAYKTLWKDLDVAYDKFIRTTDPKHEAIVKEF 180

Query: 181 YSKVLANGDIYRADYEGLYCVNCEEYKDEKELLANNCCPIHLKPCASRKEDNYFFALSKY 240
           YSKVLANGDIYRADYEGLYCVNCEEYKDEKELLA+NCCPIHLKPCASRKEDNYFFALSKY
Sbjct: 181 YSKVLANGDIYRADYEGLYCVNCEEYKDEKELLADNCCPIHLKPCASRKEDNYFFALSKY 240

Query: 241 QGLLEETLTQNPGFVQPAFRLNEVQSWIRSGLKDFSISRASVEWGIPVPNDQKQTIYVWF 300
           Q LLEETLTQNPGFVQPAFRLNEVQSWIRSGLKDFSISRASVEWGIPVPND+KQTIYVWF
Sbjct: 241 QRLLEETLTQNPGFVQPAFRLNEVQSWIRSGLKDFSISRASVEWGIPVPNDKKQTIYVWF 300

Query: 301 DALLGYISALSENGQQQNLQRAVASGWPASLHLIGKDILRFHAVYWPAMLMSAGLKLPKM 360
           DALLGYISALSENG+Q NL + VASGWPASLHLIGKDILRFHAVYWPAMLMSAGLKLPKM
Sbjct: 301 DALLGYISALSENGEQPNLHKVVASGWPASLHLIGKDILRFHAVYWPAMLMSAGLKLPKM 360

Query: 361 VFGHGFLTKDGMKMGKSLGNTLEPNELVQKFGSDAVRYFFLQEVEFGSDGDYAEDRFINI 420
           VFGHGFLTKDGMKMGKSLGNTLEPNELV+KFGSDAVRYFFL+EVEFGSDGDYAEDRFINI
Sbjct: 361 VFGHGFLTKDGMKMGKSLGNTLEPNELVEKFGSDAVRYFFLREVEFGSDGDYAEDRFINI 420

Query: 421 VNAHLANTIGNLLNRTLGLLKKNCQSTLVVDSTAAAEGNSFKDKVEELVLKARNHYEGLS 480
           VNAHLANTIGNLLNRTLGLLKKNCQSTL+VDSTAAA+GN+FKDKVEELV+KARNHYE L+
Sbjct: 421 VNAHLANTIGNLLNRTLGLLKKNCQSTLLVDSTAAADGNAFKDKVEELVVKARNHYEELA 480

Query: 481 LSSACEAVLEIGNAGNSYMDEQAPWSLFKQGGASSDAAAKDLVLILEAMRIIAVALSPVT 540
           LSSACEAVLEIGNAGNSYMDE+APWSLFKQGGASS+AAAKDLVLILEAMRIIA+ALSPVT
Sbjct: 481 LSSACEAVLEIGNAGNSYMDERAPWSLFKQGGASSNAAAKDLVLILEAMRIIAIALSPVT 540

Query: 541 PCLGWRIYAQLGFSKDQFEAATWNETKWGGLKAGQVMAPPNPVFARIEQSTGSDEDEVPK 600
           P L WRIYAQLG+SKDQF+AATWNETKWGGLKAGQVMAPPNPVFARIEQ+  + E+EVPK
Sbjct: 541 PRLSWRIYAQLGYSKDQFDAATWNETKWGGLKAGQVMAPPNPVFARIEQAIVNGEEEVPK 600

Query: 601 KPVKSKKKIAQGVAEA 617
           KPVKSKKKIAQGVAEA
Sbjct: 601 KPVKSKKKIAQGVAEA 611

BLAST of Moc04g34620 vs. NCBI nr
Match: XP_022960433.1 (methionine--tRNA ligase, chloroplastic/mitochondrial [Cucurbita moschata])

HSP 1 Score: 1108.2 bits (2865), Expect = 0.0e+00
Identity = 558/617 (90.44%), Postives = 581/617 (94.17%), Query Frame = 0

Query: 1   MAARINSSIQSSLWLLNPLQSSLNLNLNLNLNLRTRLNFYRNLLPSP-SYSASSSKRAIF 60
           MAARIN+SIQ+SLWLLNPLQSS NL      +LRTR NFY+NLL +P S S+SSSKRAIF
Sbjct: 1   MAARINTSIQNSLWLLNPLQSSRNLKAT---HLRTRFNFYQNLLSAPSSCSSSSSKRAIF 60

Query: 61  CTYASNCNSHESGAATDPFVITTPLYYVNAAPHMGSAYTTIAADAIARFQRLLGKKVIFI 120
           C+ ASN NSH+ GAATDPFVITTPLYYVNAAPHMGSAYTTIAADAIARFQRLLGKKVIFI
Sbjct: 61  CSSASNFNSHDGGAATDPFVITTPLYYVNAAPHMGSAYTTIAADAIARFQRLLGKKVIFI 120

Query: 121 TGTDEHGEKIATAAAAQGSAPSEHCDAISEAYKTLWKDLDVAYDKFIRTTDPKHEAIVKE 180
           TGTDEHGEKIATAAAAQGS PSEHCD ISEAYKTLWKDLD+AYDKFIRTTDPKHE IVKE
Sbjct: 121 TGTDEHGEKIATAAAAQGSGPSEHCDNISEAYKTLWKDLDIAYDKFIRTTDPKHETIVKE 180

Query: 181 FYSKVLANGDIYRADYEGLYCVNCEEYKDEKELLANNCCPIHLKPCASRKEDNYFFALSK 240
           FYS VLANGDIYRADYEGLYCVNCEEYKDEKELLA+NCCPIHLKPCASRKEDNYFFALSK
Sbjct: 181 FYSNVLANGDIYRADYEGLYCVNCEEYKDEKELLADNCCPIHLKPCASRKEDNYFFALSK 240

Query: 241 YQGLLEETLTQNPGFVQPAFRLNEVQSWIRSGLKDFSISRASVEWGIPVPNDQKQTIYVW 300
           YQ LLEETLTQNPGFVQPAFRLNEVQSWI+SGLKDFSISRASVEWGIPVPNDQKQTIYVW
Sbjct: 241 YQRLLEETLTQNPGFVQPAFRLNEVQSWIKSGLKDFSISRASVEWGIPVPNDQKQTIYVW 300

Query: 301 FDALLGYISALSENGQQQNLQRAVASGWPASLHLIGKDILRFHAVYWPAMLMSAGLKLPK 360
           FDALLGYISALSEN +Q NLQ AVASGWPASLHLIGKDILRFHAVYWPAMLMSAGLKLPK
Sbjct: 301 FDALLGYISALSENDEQPNLQTAVASGWPASLHLIGKDILRFHAVYWPAMLMSAGLKLPK 360

Query: 361 MVFGHGFLTKDGMKMGKSLGNTLEPNELVQKFGSDAVRYFFLQEVEFGSDGDYAEDRFIN 420
           MVFGHGFLTKDGMKMGKSLGNTLEPNELVQKFGSD VRYFFL+EVEFGSDGDYAEDRFIN
Sbjct: 361 MVFGHGFLTKDGMKMGKSLGNTLEPNELVQKFGSDPVRYFFLREVEFGSDGDYAEDRFIN 420

Query: 421 IVNAHLANTIGNLLNRTLGLLKKNCQSTLVVDSTAAAEGNSFKDKVEELVLKARNHYEGL 480
           IVNAHLANTIGNLLNRTLGLLKKNCQSTLVVDS+AAA GN+FK+KVEELV KARNHYE L
Sbjct: 421 IVNAHLANTIGNLLNRTLGLLKKNCQSTLVVDSSAAAVGNAFKEKVEELVAKARNHYEEL 480

Query: 481 SLSSACEAVLEIGNAGNSYMDEQAPWSLFKQGGASSDAAAKDLVLILEAMRIIAVALSPV 540
           SLSSACEAVLEIGNAGNSYMDE+APWSLFKQGGASSDAAAKDLVLILEAMRIIA+ALSPV
Sbjct: 481 SLSSACEAVLEIGNAGNSYMDERAPWSLFKQGGASSDAAAKDLVLILEAMRIIAIALSPV 540

Query: 541 TPCLGWRIYAQLGFSKDQFEAATWNETKWGGLKAGQVMAPPNPVFARIEQSTGSDEDEVP 600
           TP L WRIY QLG+ +DQF AATWNETKWGGLKAGQVMAPPNPVFARIEQ+  + E+E P
Sbjct: 541 TPRLSWRIYEQLGYPEDQFAAATWNETKWGGLKAGQVMAPPNPVFARIEQAIVNGEEEAP 600

Query: 601 KKPVKSKKKIAQGVAEA 617
           KKPVKSKKKIAQ VAEA
Sbjct: 601 KKPVKSKKKIAQKVAEA 614

BLAST of Moc04g34620 vs. ExPASy Swiss-Prot
Match: Q9M2T9 (Methionine--tRNA ligase, chloroplastic/mitochondrial OS=Arabidopsis thaliana OX=3702 GN=OVA1 PE=2 SV=1)

HSP 1 Score: 911.4 bits (2354), Expect = 5.6e-264
Identity = 461/624 (73.88%), Postives = 524/624 (83.97%), Query Frame = 0

Query: 1   MAARINSSIQSSLWLLNPLQSSLNLNLNLNLNLRTRLNFYRNLLPSPSYSA-SSSKRAIF 60
           MAARIN+S+ ++L  L P  + LN           R N +R     P YS  SS KRA++
Sbjct: 1   MAARINTSLHNALSFLKPFNTPLNTK-----PFSFRRNSFRFSKKLPYYSQFSSGKRALY 60

Query: 61  CTYASNCNSHESGAATDPFVITTPLYYVNAAPHMGSAYTTIAADAIARFQRLLGKKVIFI 120
           CT +S  ++ + G   + FV+TTPLYYVNA PHMGSAYTTIAAD+IARFQRLLGKKVIFI
Sbjct: 61  CTSSSQESTVDEG---ETFVLTTPLYYVNAPPHMGSAYTTIAADSIARFQRLLGKKVIFI 120

Query: 121 TGTDEHGEKIATAAAAQGSAPSEHCDAISEAYKTLWKDLDVAYDKFIRTTDPKHEAIVKE 180
           TGTDEHGEKIAT+AAA G  P EHCD IS++Y+TLWKDLD+AYDKFIRTTDPKHEAIVKE
Sbjct: 121 TGTDEHGEKIATSAAANGRNPPEHCDLISQSYRTLWKDLDIAYDKFIRTTDPKHEAIVKE 180

Query: 181 FYSKVLANGDIYRADYEGLYCVNCEEYKDEKELLANNCCPIHLKPCASRKEDNYFFALSK 240
           FY++V ANGDIYRADYEGLYCVNCEEYKDEKELL NNCCP+H  PC +RKEDNYFFALSK
Sbjct: 181 FYARVFANGDIYRADYEGLYCVNCEEYKDEKELLENNCCPVHQMPCVARKEDNYFFALSK 240

Query: 241 YQGLLEETLTQNPGFVQPAFRLNEVQSWIRSGLKDFSISRASVEWGIPVPNDQKQTIYVW 300
           YQ  LE+ L QNP FVQP++RLNEVQSWI+SGL+DFSISRA V+WGIPVP+D KQTIYVW
Sbjct: 241 YQKPLEDILAQNPRFVQPSYRLNEVQSWIKSGLRDFSISRALVDWGIPVPDDDKQTIYVW 300

Query: 301 FDALLGYISALSENGQQQNLQRAVASGWPASLHLIGKDILRFHAVYWPAMLMSAGLKLPK 360
           FDALLGYISAL+E+ +QQNL+ AV+ GWPASLHLIGKDILRFHAVYWPAMLMSAGL+LPK
Sbjct: 301 FDALLGYISALTEDNKQQNLETAVSFGWPASLHLIGKDILRFHAVYWPAMLMSAGLELPK 360

Query: 361 MVFGHGFLTKDGMKMGKSLGNTLEPNELVQKFGSDAVRYFFLQEVEFGSDGDYAEDRFIN 420
           MVFGHGFLTKDGMKMGKSLGNTLEP ELVQKFG DAVRYFFL+EVEFG+DGDY+EDRFI 
Sbjct: 361 MVFGHGFLTKDGMKMGKSLGNTLEPFELVQKFGPDAVRYFFLREVEFGNDGDYSEDRFIK 420

Query: 421 IVNAHLANTIGNLLNRTLGLLKKNCQSTLVVDSTAAAEGNSFKDKVEELVLKARNHYEGL 480
           IVNAHLANTIGNLLNRTLGLLKKNC+STLVVDST AAEG   KD VE+LV KAR +YE L
Sbjct: 421 IVNAHLANTIGNLLNRTLGLLKKNCESTLVVDSTVAAEGVPLKDTVEKLVEKARTNYENL 480

Query: 481 SLSSACEAVLEIGNAGNSYMDEQAPWSLFKQGGASSDAAAKDLVLILEAMRIIAVALSPV 540
           SLSSACEAVLEIGNAGN+YMD++APW LFKQGG S++ AAKDLV+ILE MR+IAVALSPV
Sbjct: 481 SLSSACEAVLEIGNAGNTYMDQRAPWFLFKQGGVSAEEAAKDLVIILEVMRVIAVALSPV 540

Query: 541 TPCLGWRIYAQLGFSKDQFEAATWNETKWGGLKAGQVMAPPNPVFARIEQSTGSDEDE-- 600
            PCL  RIY+QLG+++DQF + TW++TKWGGLK GQVM   +PVFARIE +   +EDE  
Sbjct: 541 APCLSLRIYSQLGYTEDQFNSITWSDTKWGGLKGGQVMEQASPVFARIELNPEKEEDEKK 600

Query: 601 --VPKKPVKSKKKIAQ---GVAEA 617
             V KK  K+K K+ +    VAEA
Sbjct: 601 PKVGKKTGKAKVKVVEQTPTVAEA 616

BLAST of Moc04g34620 vs. ExPASy Swiss-Prot
Match: Q55729 (Methionine--tRNA ligase OS=Synechocystis sp. (strain PCC 6803 / Kazusa) OX=1111708 GN=metG PE=3 SV=1)

HSP 1 Score: 560.8 bits (1444), Expect = 1.9e-158
Identity = 276/521 (52.98%), Postives = 357/521 (68.52%), Query Frame = 0

Query: 78  FVITTPLYYVNAAPHMGSAYTTIAADAIARFQRLLGKKVIFITGTDEHGEKIATAAAAQG 137
           F +TTPLYYVN  PH+GSAYTT+ AD +ARF+RL G  V+ ITGTDEHG+KI   A AQ 
Sbjct: 9   FSVTTPLYYVNDVPHLGSAYTTVVADTLARFKRLQGYDVLMITGTDEHGQKIQRTAEAQE 68

Query: 138 SAPSEHCDAISEAYKTLWKDLDVAYDKFIRTTDPKHEAIVKEFYSKVLANGDIYRADYEG 197
             P  HCD     +K LW+ L++ YD+F RTTDP+H AIVK+F+ +V   GDIY A  +G
Sbjct: 69  LDPQTHCDRTVVKFKELWRSLNILYDRFSRTTDPRHLAIVKDFFQRVWDKGDIYLAQQQG 128

Query: 198 LYCVNCEEYKDEKELLANNCCPIHLKPCAS-RKEDNYFFALSKYQGLLEETLTQNPGFVQ 257
            YCV CEE+K++++LL +N CP+H    A  R E+NYFF LS+YQ  LEE   Q P F+Q
Sbjct: 129 WYCVACEEFKEKRDLLEDNHCPLHPNRKAEWRDEENYFFRLSRYQHPLEELYAQRPEFIQ 188

Query: 258 PAFRLNEVQSWIRSGLKDFSISRASVEWGIPVPNDQKQTIYVWFDALLGYISALSENGQQ 317
           P+ R NEV +++  GL+DFSISR +++WG P+PND   TIYVWFDALLGY++AL +  ++
Sbjct: 189 PSSRRNEVLNFVAQGLQDFSISRVNLDWGFPLPNDPNHTIYVWFDALLGYVTALLDEDEE 248

Query: 318 QNLQRAVASGWPASLHLIGKDILRFHAVYWPAMLMSAGLKLPKMVFGHGFLTKDGMKMGK 377
            NL  A+   WP +LHLIGKDILRFHAVYWPAMLMSA L +P  VFGHGFLTKDG KMGK
Sbjct: 249 PNLTNALVKWWPINLHLIGKDILRFHAVYWPAMLMSAELAIPAQVFGHGFLTKDGQKMGK 308

Query: 378 SLGNTLEPNELVQKFGSDAVRYFFLQEVEFGSDGDYAEDRFINIVNAHLANTIGNLLNRT 437
           SLGNT++P +L+ ++G DA RY+FL+E+EFG DGD+ E RF+N++NA LAN +GNLLNRT
Sbjct: 309 SLGNTVDPLDLINRYGEDAFRYYFLKEIEFGKDGDFNEQRFVNVLNADLANDLGNLLNRT 368

Query: 438 LGLLKKNCQST-LVVDSTAAAEGNSFKDKVEELVLKARNHYEGLSLSSACEAVLEIGNAG 497
           LG++KK CQ     V +T  A  N  K     L  +  + YE LS + ACEA+  +  AG
Sbjct: 369 LGMVKKYCQGQGPQVMATDLAPDNPLKALGSHLGEEVSSAYERLSFTDACEAIFTLVRAG 428

Query: 498 NSYMDEQAPWSLFKQGGASSDAAAKDLVLILEAMRIIAVALSPVTPCLGWRIYAQLGFSK 557
           N Y+D+ APW LFKQG  S       L  +LE++R+ A  LSP+ P L  +IY QLGF+ 
Sbjct: 429 NKYIDDMAPWKLFKQG--SQKEVEDVLYSVLESIRLSAYLLSPIVPRLSTKIYQQLGFTW 488

Query: 558 D-------QFEAATWN-ETKWGGLKAGQVMAPPNPVFARIE 589
           D         +A  +N    WG L A Q + P  P+F ++E
Sbjct: 489 DFDQWRSPLEQAEEFNRHQSWGQLGANQNLPPAQPIFTKLE 527

BLAST of Moc04g34620 vs. ExPASy Swiss-Prot
Match: Q8Z068 (Methionine--tRNA ligase OS=Nostoc sp. (strain PCC 7120 / SAG 25.82 / UTEX 2576) OX=103690 GN=metG PE=3 SV=1)

HSP 1 Score: 556.6 bits (1433), Expect = 3.5e-157
Identity = 277/523 (52.96%), Postives = 363/523 (69.41%), Query Frame = 0

Query: 74  ATDPFVITTPLYYVNAAPHMGSAYTTIAADAIARFQRLLGKKVIFITGTDEHGEKIATAA 133
           A   F +TTPLYYVN  PH+GSAYTT+AADA+ARFQ+LLG+ V+ ITGTDEHG+KI  +A
Sbjct: 7   AEKTFALTTPLYYVNDVPHIGSAYTTMAADAVARFQKLLGRDVLLITGTDEHGQKIQRSA 66

Query: 134 AAQGSAPSEHCDAISEAYKTLWKDLDVAYDKFIRTTDPKHEAIVKEFYSKVLANGDIYRA 193
            + G AP E CD I  ++ +LW+ L++ YD+F RTT  +H+AIV EF+++V   GDIY+ 
Sbjct: 67  ESLGKAPQEFCDEIVPSFMSLWRLLNIQYDRFSRTTAVRHKAIVDEFFARVWEAGDIYQG 126

Query: 194 DYEGLYCVNCEEYKDEKELLANNCCPIHL-KPCASRKEDNYFFALSKYQGLLEETLTQNP 253
             +G YCV+CEE+K+E+ELL  N CPIH+ K    R E NYFF LSKYQ  LEE    +P
Sbjct: 127 QQKGWYCVSCEEFKEERELLEGNRCPIHVNKEVEWRDEQNYFFRLSKYQTQLEEFYQSHP 186

Query: 254 GFVQPAFRLNEVQSWIRSGLKDFSISRASVEWGIPVPNDQKQTIYVWFDALLGYISALSE 313
            F+QP  R NEV +++  GL+DFSISR +++WG PVPND K T+YVWFDALL Y++AL +
Sbjct: 187 DFIQPESRRNEVLNFVSQGLQDFSISRVNLDWGFPVPNDPKHTLYVWFDALLAYVTALLD 246

Query: 314 NGQQQNLQRAVASGWPASLHLIGKDILRFHAVYWPAMLMSAGLKLPKMVFGHGFLTKDGM 373
              +  L+ A+   WP +LHLIGKDILRFHAVYWPAML+SAGL LP  VFGHGFLTKDG 
Sbjct: 247 PEDEPTLENALGKWWPINLHLIGKDILRFHAVYWPAMLLSAGLPLPDRVFGHGFLTKDGQ 306

Query: 374 KMGKSLGNTLEPNELVQKFGSDAVRYFFLQEVEFGSDGDYAEDRFINIVNAHLANTIGNL 433
           KMGKSLGNT++P  LVQ++GSDAVRY+FL+E+EFG DGD+ E RFI+++NA LAN +GNL
Sbjct: 307 KMGKSLGNTVDPVGLVQQYGSDAVRYYFLKEIEFGKDGDFNEVRFIHVLNADLANDLGNL 366

Query: 434 LNRTLGLLKKNCQS-TLVVDSTAAAEGNSFKDKVEELVLKARNHYEGLSLSSACEAVLEI 493
           LNRTL ++KK C +  L + +      N+ K    +L  K +  YE L+ + AC  VL +
Sbjct: 367 LNRTLNMVKKYCANYALSITNEDIPAENTLKALGVDLGAKVKQAYEVLAFNQACGVVLSL 426

Query: 494 GNAGNSYMDEQAPWSLFKQGGASSDAAAKDLVLILEAMRIIAVALSPVTPCLGWRIYAQL 553
             A N ++D+QAPWSL+KQ           L  +LE++R+ A  LSPV P +   IY QL
Sbjct: 427 VQASNKFIDDQAPWSLYKQ--ERQQEVETVLYTVLESVRLAAYLLSPVIPNISSNIYQQL 486

Query: 554 GFS---KDQFEAATWN-ETKWGGLKAGQVMAPPNPVFARIEQS 591
           GF     +Q +   +    +WG L   Q +  P P+F RIEQ+
Sbjct: 487 GFGINFNEQTDITPFAIHAQWGLLSNKQQLGQPQPIFKRIEQT 527

BLAST of Moc04g34620 vs. ExPASy Swiss-Prot
Match: P59081 (Methionine--tRNA ligase OS=Thermosynechococcus elongatus (strain BP-1) OX=197221 GN=metG PE=3 SV=1)

HSP 1 Score: 531.2 bits (1367), Expect = 1.6e-149
Identity = 263/518 (50.77%), Postives = 346/518 (66.80%), Query Frame = 0

Query: 78  FVITTPLYYVNAAPHMGSAYTTIAADAIARFQRLLGKKVIFITGTDEHGEKIATAAAAQG 137
           F +TTPLYYVNA PH+GSAYTTIAAD +ARF RL G +V FITGTDEHG+KI   A  +G
Sbjct: 5   FSLTTPLYYVNALPHIGSAYTTIAADVLARFYRLQGYQVRFITGTDEHGQKIERTAQQRG 64

Query: 138 SAPSEHCDAISEAYKTLWKDLDVAYDKFIRTTDPKHEAIVKEFYSKVLANGDIYRADYEG 197
            +P  HCD I+  ++ LW+ L++ YD+F RTT P+H AIV EF+ +V  NGDIY    +G
Sbjct: 65  LSPQAHCDEIAAGFQALWQQLNIHYDRFSRTTSPRHHAIVNEFFQRVWDNGDIYLGQQQG 124

Query: 198 LYCVNCEEYKDEKELLANNCCPIHLKPCAS-RKEDNYFFALSKYQGLLEETLTQNPGFVQ 257
            YCV CEE+K+E+ELL    CPIH+      R E NYFF LSKYQ  L +   ++P FVQ
Sbjct: 125 WYCVECEEFKEERELLEGRRCPIHVNRTVEWRDERNYFFRLSKYQQALLDHYAEHPDFVQ 184

Query: 258 PAFRLNEVQSWIRSGLKDFSISRASVEWGIPVPNDQKQTIYVWFDALLGYISALSENGQQ 317
           P  R NEV S+I  GL+DFSISR ++ WG PVP D +QT+YVWFDALLGY++AL E   +
Sbjct: 185 PPSRRNEVLSFIERGLQDFSISRVNLAWGFPVPTDPEQTLYVWFDALLGYVTALLEPEDE 244

Query: 318 QNLQRAVASGWPASLHLIGKDILRFHAVYWPAMLMSAGLKLPKMVFGHGFLTKDGMKMGK 377
             L  A+ + WP +LH+IGKDILRFH + WPAMLMSAGL LP+ +F HGFLTKDG KMGK
Sbjct: 245 PTLANALKTWWPINLHIIGKDILRFHGISWPAMLMSAGLPLPEQIFVHGFLTKDGQKMGK 304

Query: 378 SLGNTLEPNELVQKFGSDAVRYFFLQEVEFGSDGDYAEDRFINIVNAHLANTIGNLLNRT 437
           SLGNTL+P  LV ++G+DAVRY+F++EVEFG DGD++E RF+ I+NA LAN +GNLLNRT
Sbjct: 305 SLGNTLDPFALVAQYGADAVRYYFMKEVEFGRDGDFSETRFVTILNADLANDLGNLLNRT 364

Query: 438 LGLLKKNCQSTLV-VDSTAAAEGNSFKDKVEELVLKARNHYEGLSLSSACEAVLEIGNAG 497
           L +  K     +  V   A    +  +   E L       Y  L+    C+  L +  AG
Sbjct: 365 LKMAWKYTDGKVPNVQGAAIPREHPLRQLAEHLSQTYGQGYRQLAFHEVCQQALTLARAG 424

Query: 498 NSYMDEQAPWSLFKQGGASSDAAAKDLVLILEAMRIIAVALSPVTPCLGWRIYAQLGFSK 557
           N ++DE+APW  ++ G   + A A+ L  +LE++R++A  L+P+ P L   IY QLG+S 
Sbjct: 425 NKFLDEEAPWKRYRAG--ETAAVAEILYCVLESVRLVAYVLAPIIPQLSEAIYGQLGYS- 484

Query: 558 DQFEAATW------NETKWGGLKAGQVMAPPNPVFARI 588
            +F  +         + +WG L A Q +A P P+F ++
Sbjct: 485 IRFNGSIAPDLLGDRQAQWGVLPASQPLANPEPIFQKL 519

BLAST of Moc04g34620 vs. ExPASy Swiss-Prot
Match: O67298 (Methionine--tRNA ligase OS=Aquifex aeolicus (strain VF5) OX=224324 GN=metG PE=1 SV=1)

HSP 1 Score: 431.8 bits (1109), Expect = 1.3e-119
Identity = 217/466 (46.57%), Postives = 291/466 (62.45%), Query Frame = 0

Query: 78  FVITTPLYYVNAAPHMGSAYTTIAADAIARFQRLLGKKVIFITGTDEHGEKIATAAAAQG 137
           F +TTP+YYVN  PH+G AYTTIAAD IAR+ RL    V F+TGTDEHG KI   A   G
Sbjct: 7   FYVTTPIYYVNDVPHLGHAYTTIAADTIARYYRLRDYDVFFLTGTDEHGLKIQKKAEELG 66

Query: 138 SAPSEHCDAISEAYKTLWKDLDVAYDKFIRTTDPKHEAIVKEFYSKVLANGDIYRADYEG 197
            +P E  D  +E +K LW+ L + Y KFIRTTDP H   V++ + +    GDIY  +YEG
Sbjct: 67  ISPKELVDRNAERFKKLWEFLKIEYTKFIRTTDPYHVKFVQKVFEECYKRGDIYLGEYEG 126

Query: 198 LYCVNCEEYKDEKELLANNCCPIHLKPCASRKEDNYFFALSKYQGLLEETLTQNPGFVQP 257
            YCV CEE+K E EL  ++ CPIH K C   KE +YFF LSKYQ  L E   +NP F+QP
Sbjct: 127 WYCVGCEEFKSEAELAEDHTCPIHQKKCEYIKEPSYFFRLSKYQDKLLELYEKNPEFIQP 186

Query: 258 AFRLNEVQSWIRSGLKDFSIS--RASVEWGIPVPNDQKQTIYVWFDALLGYISALSENGQ 317
            +R NE+ S+++ GLKD S++  R+ V+WGIPVP D + TIYVWFDAL  YISAL +   
Sbjct: 187 DYRRNEIISFVKQGLKDLSVTRPRSRVKWGIPVPFDPEHTIYVWFDALFNYISALEDK-- 246

Query: 318 QQNLQRAVASGWPASLHLIGKDILRFHAVYWPAMLMSAGLKLPKMVFGHGFLTKDGMKMG 377
                  V   WPA LHL+GKDILRFH VYWPA LMS G +LPK VF HG+ T +G KM 
Sbjct: 247 -------VEIYWPADLHLVGKDILRFHTVYWPAFLMSLGYELPKKVFAHGWWTVEGKKMS 306

Query: 378 KSLGNTLEPNELVQKFGSDAVRYFFLQEVEFGSDGDYAEDRFINIVNAHLANTIGNLLNR 437
           K+LGN ++P E+VQ++G D VRYF L+EV FG DGD+++   +N +N  LAN IGNL +R
Sbjct: 307 KTLGNVVDPYEVVQEYGLDEVRYFLLREVPFGQDGDFSKKAILNRINGELANEIGNLYSR 366

Query: 438 TLGLLKKNCQSTLVVDSTAAAEGNSFKDKVEELVLKARNHYEGLSLSSACEAVLEIGNAG 497
            + +  K      +    + A    +    +E +    N+ E ++   A E +L+  +  
Sbjct: 367 VVNMAHK-----FLGGEVSGARDEEYAKIAQESIKNYENYMEKVNFYKAIEEILKFTSYL 426

Query: 498 NSYMDEQAPWSLFKQGGASSDAAAKDLVLILEAMRIIAVALSPVTP 542
           N Y+DE+ PW+L K+     +   K L  +++ + ++   L P+TP
Sbjct: 427 NKYVDEKQPWALNKE--RKKEELQKVLYALVDGLFVLTHLLYPITP 456

BLAST of Moc04g34620 vs. ExPASy TrEMBL
Match: A0A6J1BXG8 (Methionine--tRNA ligase OS=Momordica charantia OX=3673 GN=LOC111006379 PE=3 SV=1)

HSP 1 Score: 1232.2 bits (3187), Expect = 0.0e+00
Identity = 616/616 (100.00%), Postives = 616/616 (100.00%), Query Frame = 0

Query: 1   MAARINSSIQSSLWLLNPLQSSLNLNLNLNLNLRTRLNFYRNLLPSPSYSASSSKRAIFC 60
           MAARINSSIQSSLWLLNPLQSSLNLNLNLNLNLRTRLNFYRNLLPSPSYSASSSKRAIFC
Sbjct: 1   MAARINSSIQSSLWLLNPLQSSLNLNLNLNLNLRTRLNFYRNLLPSPSYSASSSKRAIFC 60

Query: 61  TYASNCNSHESGAATDPFVITTPLYYVNAAPHMGSAYTTIAADAIARFQRLLGKKVIFIT 120
           TYASNCNSHESGAATDPFVITTPLYYVNAAPHMGSAYTTIAADAIARFQRLLGKKVIFIT
Sbjct: 61  TYASNCNSHESGAATDPFVITTPLYYVNAAPHMGSAYTTIAADAIARFQRLLGKKVIFIT 120

Query: 121 GTDEHGEKIATAAAAQGSAPSEHCDAISEAYKTLWKDLDVAYDKFIRTTDPKHEAIVKEF 180
           GTDEHGEKIATAAAAQGSAPSEHCDAISEAYKTLWKDLDVAYDKFIRTTDPKHEAIVKEF
Sbjct: 121 GTDEHGEKIATAAAAQGSAPSEHCDAISEAYKTLWKDLDVAYDKFIRTTDPKHEAIVKEF 180

Query: 181 YSKVLANGDIYRADYEGLYCVNCEEYKDEKELLANNCCPIHLKPCASRKEDNYFFALSKY 240
           YSKVLANGDIYRADYEGLYCVNCEEYKDEKELLANNCCPIHLKPCASRKEDNYFFALSKY
Sbjct: 181 YSKVLANGDIYRADYEGLYCVNCEEYKDEKELLANNCCPIHLKPCASRKEDNYFFALSKY 240

Query: 241 QGLLEETLTQNPGFVQPAFRLNEVQSWIRSGLKDFSISRASVEWGIPVPNDQKQTIYVWF 300
           QGLLEETLTQNPGFVQPAFRLNEVQSWIRSGLKDFSISRASVEWGIPVPNDQKQTIYVWF
Sbjct: 241 QGLLEETLTQNPGFVQPAFRLNEVQSWIRSGLKDFSISRASVEWGIPVPNDQKQTIYVWF 300

Query: 301 DALLGYISALSENGQQQNLQRAVASGWPASLHLIGKDILRFHAVYWPAMLMSAGLKLPKM 360
           DALLGYISALSENGQQQNLQRAVASGWPASLHLIGKDILRFHAVYWPAMLMSAGLKLPKM
Sbjct: 301 DALLGYISALSENGQQQNLQRAVASGWPASLHLIGKDILRFHAVYWPAMLMSAGLKLPKM 360

Query: 361 VFGHGFLTKDGMKMGKSLGNTLEPNELVQKFGSDAVRYFFLQEVEFGSDGDYAEDRFINI 420
           VFGHGFLTKDGMKMGKSLGNTLEPNELVQKFGSDAVRYFFLQEVEFGSDGDYAEDRFINI
Sbjct: 361 VFGHGFLTKDGMKMGKSLGNTLEPNELVQKFGSDAVRYFFLQEVEFGSDGDYAEDRFINI 420

Query: 421 VNAHLANTIGNLLNRTLGLLKKNCQSTLVVDSTAAAEGNSFKDKVEELVLKARNHYEGLS 480
           VNAHLANTIGNLLNRTLGLLKKNCQSTLVVDSTAAAEGNSFKDKVEELVLKARNHYEGLS
Sbjct: 421 VNAHLANTIGNLLNRTLGLLKKNCQSTLVVDSTAAAEGNSFKDKVEELVLKARNHYEGLS 480

Query: 481 LSSACEAVLEIGNAGNSYMDEQAPWSLFKQGGASSDAAAKDLVLILEAMRIIAVALSPVT 540
           LSSACEAVLEIGNAGNSYMDEQAPWSLFKQGGASSDAAAKDLVLILEAMRIIAVALSPVT
Sbjct: 481 LSSACEAVLEIGNAGNSYMDEQAPWSLFKQGGASSDAAAKDLVLILEAMRIIAVALSPVT 540

Query: 541 PCLGWRIYAQLGFSKDQFEAATWNETKWGGLKAGQVMAPPNPVFARIEQSTGSDEDEVPK 600
           PCLGWRIYAQLGFSKDQFEAATWNETKWGGLKAGQVMAPPNPVFARIEQSTGSDEDEVPK
Sbjct: 541 PCLGWRIYAQLGFSKDQFEAATWNETKWGGLKAGQVMAPPNPVFARIEQSTGSDEDEVPK 600

Query: 601 KPVKSKKKIAQGVAEA 617
           KPVKSKKKIAQGVAEA
Sbjct: 601 KPVKSKKKIAQGVAEA 616

BLAST of Moc04g34620 vs. ExPASy TrEMBL
Match: A0A0A0KQV7 (Methionine--tRNA ligase OS=Cucumis sativus OX=3659 GN=Csa_5G512910 PE=3 SV=1)

HSP 1 Score: 1110.9 bits (2872), Expect = 0.0e+00
Identity = 557/616 (90.42%), Postives = 584/616 (94.81%), Query Frame = 0

Query: 1   MAARINSSIQSSLWLLNPLQSSLNLNLNLNLNLRTRLNFYRNLLPSPSYSASSSKRAIFC 60
           MAARIN+SIQ+SLWLLNP  SSLNL      + RTRL+F +NLL  PS+  SSSKRAIFC
Sbjct: 1   MAARINTSIQTSLWLLNPFHSSLNLK---PTHFRTRLHFCQNLLSFPSH--SSSKRAIFC 60

Query: 61  TYASNCNSHESGAATDPFVITTPLYYVNAAPHMGSAYTTIAADAIARFQRLLGKKVIFIT 120
           T  SN NSH+SGAATDPFVITTPLYYVNAAPHMGSAYTTIAADA+ARFQRLLGKKVIFIT
Sbjct: 61  TSTSNFNSHDSGAATDPFVITTPLYYVNAAPHMGSAYTTIAADAVARFQRLLGKKVIFIT 120

Query: 121 GTDEHGEKIATAAAAQGSAPSEHCDAISEAYKTLWKDLDVAYDKFIRTTDPKHEAIVKEF 180
           GTDEHGEKIATAAA QGS PSEHCD ISEAYKTLWKDLDVAYDKFIRTTDPKHEAIVKEF
Sbjct: 121 GTDEHGEKIATAAATQGSTPSEHCDIISEAYKTLWKDLDVAYDKFIRTTDPKHEAIVKEF 180

Query: 181 YSKVLANGDIYRADYEGLYCVNCEEYKDEKELLANNCCPIHLKPCASRKEDNYFFALSKY 240
           YSKVLANGDIYRADYEGLYCVNCEEYKDEKELLA+NCCPIHLKPCASRKEDNYFFALSKY
Sbjct: 181 YSKVLANGDIYRADYEGLYCVNCEEYKDEKELLADNCCPIHLKPCASRKEDNYFFALSKY 240

Query: 241 QGLLEETLTQNPGFVQPAFRLNEVQSWIRSGLKDFSISRASVEWGIPVPNDQKQTIYVWF 300
           Q LLEETLTQNPGFVQPAFRLNEVQSWIRSGLKDFSISRASVEWGIPVPND+KQTIYVWF
Sbjct: 241 QRLLEETLTQNPGFVQPAFRLNEVQSWIRSGLKDFSISRASVEWGIPVPNDEKQTIYVWF 300

Query: 301 DALLGYISALSENGQQQNLQRAVASGWPASLHLIGKDILRFHAVYWPAMLMSAGLKLPKM 360
           DALLGYISALSENG+Q NLQ  VASGWPASLHLIGKDILRFHAVYWPAMLMSAGLKLPKM
Sbjct: 301 DALLGYISALSENGEQPNLQEVVASGWPASLHLIGKDILRFHAVYWPAMLMSAGLKLPKM 360

Query: 361 VFGHGFLTKDGMKMGKSLGNTLEPNELVQKFGSDAVRYFFLQEVEFGSDGDYAEDRFINI 420
           VFGHGFLTKDGMKMGKSLGNTLEPNELV+KFGSDAVRYFFL+EVEFGSDGDYAEDRFINI
Sbjct: 361 VFGHGFLTKDGMKMGKSLGNTLEPNELVEKFGSDAVRYFFLREVEFGSDGDYAEDRFINI 420

Query: 421 VNAHLANTIGNLLNRTLGLLKKNCQSTLVVDSTAAAEGNSFKDKVEELVLKARNHYEGLS 480
           VNAHLANTIGNLLNRTLGLLKKNCQSTLVVDSTAAA+GN+FKDKVEELV+KARN+YE L+
Sbjct: 421 VNAHLANTIGNLLNRTLGLLKKNCQSTLVVDSTAAADGNAFKDKVEELVVKARNNYEELA 480

Query: 481 LSSACEAVLEIGNAGNSYMDEQAPWSLFKQGGASSDAAAKDLVLILEAMRIIAVALSPVT 540
           LSSACEAVLEIGNAGNSYMDE+APWSLFKQGGASS+AAAKDLVLILEAMRIIA+ALSPVT
Sbjct: 481 LSSACEAVLEIGNAGNSYMDERAPWSLFKQGGASSNAAAKDLVLILEAMRIIAIALSPVT 540

Query: 541 PCLGWRIYAQLGFSKDQFEAATWNETKWGGLKAGQVMAPPNPVFARIEQSTGSDEDEVPK 600
           P L WRIYAQLG+SKDQF+AATWNETKWGGLKAGQVMAPPNPVFARIEQ+  + E+E PK
Sbjct: 541 PRLSWRIYAQLGYSKDQFDAATWNETKWGGLKAGQVMAPPNPVFARIEQAIVNGEEEAPK 600

Query: 601 KPVKSKKKIAQGVAEA 617
           KPVKSKKKIAQ VAE+
Sbjct: 601 KPVKSKKKIAQAVAES 611

BLAST of Moc04g34620 vs. ExPASy TrEMBL
Match: A0A5D3C1K0 (Methionine--tRNA ligase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold98G003330 PE=3 SV=1)

HSP 1 Score: 1108.6 bits (2866), Expect = 0.0e+00
Identity = 557/616 (90.42%), Postives = 584/616 (94.81%), Query Frame = 0

Query: 1   MAARINSSIQSSLWLLNPLQSSLNLNLNLNLNLRTRLNFYRNLLPSPSYSASSSKRAIFC 60
           MAARIN+SIQ+SL LLNP  +SLNL      + RTRL+FY+NLL   SY  SSSKRAIFC
Sbjct: 1   MAARINTSIQTSLSLLNPFHNSLNLK---PTHFRTRLHFYQNLLSFASY--SSSKRAIFC 60

Query: 61  TYASNCNSHESGAATDPFVITTPLYYVNAAPHMGSAYTTIAADAIARFQRLLGKKVIFIT 120
           T  SN NSH++GAATDPFVITTPLYYVNAAPHMGSAYTTIAADAIARFQRLLGKKVI IT
Sbjct: 61  TTTSNFNSHDTGAATDPFVITTPLYYVNAAPHMGSAYTTIAADAIARFQRLLGKKVILIT 120

Query: 121 GTDEHGEKIATAAAAQGSAPSEHCDAISEAYKTLWKDLDVAYDKFIRTTDPKHEAIVKEF 180
           GTDEHGEKIATAAA QGS PSEHCD ISEAYKTLWKDLDVAYDKFIRTTDPKHEAIVKEF
Sbjct: 121 GTDEHGEKIATAAATQGSTPSEHCDIISEAYKTLWKDLDVAYDKFIRTTDPKHEAIVKEF 180

Query: 181 YSKVLANGDIYRADYEGLYCVNCEEYKDEKELLANNCCPIHLKPCASRKEDNYFFALSKY 240
           YSKVLANGDIYRADYEGLYCVNCEEYKDEKELLA+NCCPIHLKPCASRKEDNYFFALSKY
Sbjct: 181 YSKVLANGDIYRADYEGLYCVNCEEYKDEKELLADNCCPIHLKPCASRKEDNYFFALSKY 240

Query: 241 QGLLEETLTQNPGFVQPAFRLNEVQSWIRSGLKDFSISRASVEWGIPVPNDQKQTIYVWF 300
           Q LLEETLTQNPGFVQPAFRLNEVQSWIRSGLKDFSISRASVEWGIPVPND+KQTIYVWF
Sbjct: 241 QRLLEETLTQNPGFVQPAFRLNEVQSWIRSGLKDFSISRASVEWGIPVPNDKKQTIYVWF 300

Query: 301 DALLGYISALSENGQQQNLQRAVASGWPASLHLIGKDILRFHAVYWPAMLMSAGLKLPKM 360
           DALLGYISALSENG+Q NL + VASGWPASLHLIGKDILRFHAVYWPAMLMSAGLKLPKM
Sbjct: 301 DALLGYISALSENGEQPNLHKVVASGWPASLHLIGKDILRFHAVYWPAMLMSAGLKLPKM 360

Query: 361 VFGHGFLTKDGMKMGKSLGNTLEPNELVQKFGSDAVRYFFLQEVEFGSDGDYAEDRFINI 420
           VFGHGFLTKDGMKMGKSLGNTLEPNELV+KFGSDAVRYFFL+EVEFGSDGDYAEDRFINI
Sbjct: 361 VFGHGFLTKDGMKMGKSLGNTLEPNELVEKFGSDAVRYFFLREVEFGSDGDYAEDRFINI 420

Query: 421 VNAHLANTIGNLLNRTLGLLKKNCQSTLVVDSTAAAEGNSFKDKVEELVLKARNHYEGLS 480
           VNAHLANTIGNLLNRTLGLLKKNCQSTL+VDSTAAA+GN+FKDKVEELV+KARNHYE L+
Sbjct: 421 VNAHLANTIGNLLNRTLGLLKKNCQSTLLVDSTAAADGNAFKDKVEELVVKARNHYEELA 480

Query: 481 LSSACEAVLEIGNAGNSYMDEQAPWSLFKQGGASSDAAAKDLVLILEAMRIIAVALSPVT 540
           LSSACEAVLEIGNAGNSYMDE+APWSLFKQGGASS+AAAKDLVLILEAMRIIA+ALSPVT
Sbjct: 481 LSSACEAVLEIGNAGNSYMDERAPWSLFKQGGASSNAAAKDLVLILEAMRIIAIALSPVT 540

Query: 541 PCLGWRIYAQLGFSKDQFEAATWNETKWGGLKAGQVMAPPNPVFARIEQSTGSDEDEVPK 600
           P L WRIYAQLG+SKDQF+AATWNETKWGGLKAGQVMAPPNPVFARIEQ+  + E+EVPK
Sbjct: 541 PRLSWRIYAQLGYSKDQFDAATWNETKWGGLKAGQVMAPPNPVFARIEQAIVNGEEEVPK 600

Query: 601 KPVKSKKKIAQGVAEA 617
           KPVKSKKKIAQGVAEA
Sbjct: 601 KPVKSKKKIAQGVAEA 611

BLAST of Moc04g34620 vs. ExPASy TrEMBL
Match: A0A6J1H8X3 (Methionine--tRNA ligase OS=Cucurbita moschata OX=3662 GN=LOC111461165 PE=3 SV=1)

HSP 1 Score: 1108.2 bits (2865), Expect = 0.0e+00
Identity = 558/617 (90.44%), Postives = 581/617 (94.17%), Query Frame = 0

Query: 1   MAARINSSIQSSLWLLNPLQSSLNLNLNLNLNLRTRLNFYRNLLPSP-SYSASSSKRAIF 60
           MAARIN+SIQ+SLWLLNPLQSS NL      +LRTR NFY+NLL +P S S+SSSKRAIF
Sbjct: 1   MAARINTSIQNSLWLLNPLQSSRNLKAT---HLRTRFNFYQNLLSAPSSCSSSSSKRAIF 60

Query: 61  CTYASNCNSHESGAATDPFVITTPLYYVNAAPHMGSAYTTIAADAIARFQRLLGKKVIFI 120
           C+ ASN NSH+ GAATDPFVITTPLYYVNAAPHMGSAYTTIAADAIARFQRLLGKKVIFI
Sbjct: 61  CSSASNFNSHDGGAATDPFVITTPLYYVNAAPHMGSAYTTIAADAIARFQRLLGKKVIFI 120

Query: 121 TGTDEHGEKIATAAAAQGSAPSEHCDAISEAYKTLWKDLDVAYDKFIRTTDPKHEAIVKE 180
           TGTDEHGEKIATAAAAQGS PSEHCD ISEAYKTLWKDLD+AYDKFIRTTDPKHE IVKE
Sbjct: 121 TGTDEHGEKIATAAAAQGSGPSEHCDNISEAYKTLWKDLDIAYDKFIRTTDPKHETIVKE 180

Query: 181 FYSKVLANGDIYRADYEGLYCVNCEEYKDEKELLANNCCPIHLKPCASRKEDNYFFALSK 240
           FYS VLANGDIYRADYEGLYCVNCEEYKDEKELLA+NCCPIHLKPCASRKEDNYFFALSK
Sbjct: 181 FYSNVLANGDIYRADYEGLYCVNCEEYKDEKELLADNCCPIHLKPCASRKEDNYFFALSK 240

Query: 241 YQGLLEETLTQNPGFVQPAFRLNEVQSWIRSGLKDFSISRASVEWGIPVPNDQKQTIYVW 300
           YQ LLEETLTQNPGFVQPAFRLNEVQSWI+SGLKDFSISRASVEWGIPVPNDQKQTIYVW
Sbjct: 241 YQRLLEETLTQNPGFVQPAFRLNEVQSWIKSGLKDFSISRASVEWGIPVPNDQKQTIYVW 300

Query: 301 FDALLGYISALSENGQQQNLQRAVASGWPASLHLIGKDILRFHAVYWPAMLMSAGLKLPK 360
           FDALLGYISALSEN +Q NLQ AVASGWPASLHLIGKDILRFHAVYWPAMLMSAGLKLPK
Sbjct: 301 FDALLGYISALSENDEQPNLQTAVASGWPASLHLIGKDILRFHAVYWPAMLMSAGLKLPK 360

Query: 361 MVFGHGFLTKDGMKMGKSLGNTLEPNELVQKFGSDAVRYFFLQEVEFGSDGDYAEDRFIN 420
           MVFGHGFLTKDGMKMGKSLGNTLEPNELVQKFGSD VRYFFL+EVEFGSDGDYAEDRFIN
Sbjct: 361 MVFGHGFLTKDGMKMGKSLGNTLEPNELVQKFGSDPVRYFFLREVEFGSDGDYAEDRFIN 420

Query: 421 IVNAHLANTIGNLLNRTLGLLKKNCQSTLVVDSTAAAEGNSFKDKVEELVLKARNHYEGL 480
           IVNAHLANTIGNLLNRTLGLLKKNCQSTLVVDS+AAA GN+FK+KVEELV KARNHYE L
Sbjct: 421 IVNAHLANTIGNLLNRTLGLLKKNCQSTLVVDSSAAAVGNAFKEKVEELVAKARNHYEEL 480

Query: 481 SLSSACEAVLEIGNAGNSYMDEQAPWSLFKQGGASSDAAAKDLVLILEAMRIIAVALSPV 540
           SLSSACEAVLEIGNAGNSYMDE+APWSLFKQGGASSDAAAKDLVLILEAMRIIA+ALSPV
Sbjct: 481 SLSSACEAVLEIGNAGNSYMDERAPWSLFKQGGASSDAAAKDLVLILEAMRIIAIALSPV 540

Query: 541 TPCLGWRIYAQLGFSKDQFEAATWNETKWGGLKAGQVMAPPNPVFARIEQSTGSDEDEVP 600
           TP L WRIY QLG+ +DQF AATWNETKWGGLKAGQVMAPPNPVFARIEQ+  + E+E P
Sbjct: 541 TPRLSWRIYEQLGYPEDQFAAATWNETKWGGLKAGQVMAPPNPVFARIEQAIVNGEEEAP 600

Query: 601 KKPVKSKKKIAQGVAEA 617
           KKPVKSKKKIAQ VAEA
Sbjct: 601 KKPVKSKKKIAQKVAEA 614

BLAST of Moc04g34620 vs. ExPASy TrEMBL
Match: A0A1S3CJS7 (Methionine--tRNA ligase OS=Cucumis melo OX=3656 GN=LOC103501636 PE=3 SV=1)

HSP 1 Score: 1107.8 bits (2864), Expect = 0.0e+00
Identity = 557/616 (90.42%), Postives = 583/616 (94.64%), Query Frame = 0

Query: 1   MAARINSSIQSSLWLLNPLQSSLNLNLNLNLNLRTRLNFYRNLLPSPSYSASSSKRAIFC 60
           MAARIN+SIQ+SL LLNP  +SLNL      + RTRL+FY+NLL   SY  SSSKRAIFC
Sbjct: 1   MAARINTSIQTSLSLLNPFHNSLNLK---PTHFRTRLHFYQNLLSFASY--SSSKRAIFC 60

Query: 61  TYASNCNSHESGAATDPFVITTPLYYVNAAPHMGSAYTTIAADAIARFQRLLGKKVIFIT 120
           T  SN NSH++GAATDPFVITTPLYYVNAAPHMGSAYTTIAADAIARFQRLLGKKVI IT
Sbjct: 61  TTTSNFNSHDTGAATDPFVITTPLYYVNAAPHMGSAYTTIAADAIARFQRLLGKKVILIT 120

Query: 121 GTDEHGEKIATAAAAQGSAPSEHCDAISEAYKTLWKDLDVAYDKFIRTTDPKHEAIVKEF 180
           GTDEHGEKIATAAA QGS PSEHCD ISEAYKTLWKDLDVAYDKFIRTTDPKHEAIVKEF
Sbjct: 121 GTDEHGEKIATAAATQGSTPSEHCDIISEAYKTLWKDLDVAYDKFIRTTDPKHEAIVKEF 180

Query: 181 YSKVLANGDIYRADYEGLYCVNCEEYKDEKELLANNCCPIHLKPCASRKEDNYFFALSKY 240
           YSKVLANGDIYRADYEGLYCVNCEEYKDEKELLA+NCCPIHLKPCASRKEDNYFFALSKY
Sbjct: 181 YSKVLANGDIYRADYEGLYCVNCEEYKDEKELLADNCCPIHLKPCASRKEDNYFFALSKY 240

Query: 241 QGLLEETLTQNPGFVQPAFRLNEVQSWIRSGLKDFSISRASVEWGIPVPNDQKQTIYVWF 300
           Q LLEETLTQNPGFVQPAFRLNEVQSWIRSGLKDFSISRASVEWGIPVPND KQTIYVWF
Sbjct: 241 QRLLEETLTQNPGFVQPAFRLNEVQSWIRSGLKDFSISRASVEWGIPVPNDXKQTIYVWF 300

Query: 301 DALLGYISALSENGQQQNLQRAVASGWPASLHLIGKDILRFHAVYWPAMLMSAGLKLPKM 360
           DALLGYISALSENG+Q NL + VASGWPASLHLIGKDILRFHAVYWPAMLMSAGLKLPKM
Sbjct: 301 DALLGYISALSENGEQPNLHKVVASGWPASLHLIGKDILRFHAVYWPAMLMSAGLKLPKM 360

Query: 361 VFGHGFLTKDGMKMGKSLGNTLEPNELVQKFGSDAVRYFFLQEVEFGSDGDYAEDRFINI 420
           VFGHGFLTKDGMKMGKSLGNTLEPNELV+KFGSDAVRYFFL+EVEFGSDGDYAEDRFINI
Sbjct: 361 VFGHGFLTKDGMKMGKSLGNTLEPNELVEKFGSDAVRYFFLREVEFGSDGDYAEDRFINI 420

Query: 421 VNAHLANTIGNLLNRTLGLLKKNCQSTLVVDSTAAAEGNSFKDKVEELVLKARNHYEGLS 480
           VNAHLANTIGNLLNRTLGLLKKNCQSTL+VDSTAAA+GN+FKDKVEELV+KARNHYE L+
Sbjct: 421 VNAHLANTIGNLLNRTLGLLKKNCQSTLLVDSTAAADGNAFKDKVEELVVKARNHYEELA 480

Query: 481 LSSACEAVLEIGNAGNSYMDEQAPWSLFKQGGASSDAAAKDLVLILEAMRIIAVALSPVT 540
           LSSACEAVLEIGNAGNSYMDE+APWSLFKQGGASS+AAAKDLVLILEAMRIIA+ALSPVT
Sbjct: 481 LSSACEAVLEIGNAGNSYMDERAPWSLFKQGGASSNAAAKDLVLILEAMRIIAIALSPVT 540

Query: 541 PCLGWRIYAQLGFSKDQFEAATWNETKWGGLKAGQVMAPPNPVFARIEQSTGSDEDEVPK 600
           P L WRIYAQLG+SKDQF+AATWNETKWGGLKAGQVMAPPNPVFARIEQ+  + E+EVPK
Sbjct: 541 PRLSWRIYAQLGYSKDQFDAATWNETKWGGLKAGQVMAPPNPVFARIEQAIVNGEEEVPK 600

Query: 601 KPVKSKKKIAQGVAEA 617
           KPVKSKKKIAQGVAEA
Sbjct: 601 KPVKSKKKIAQGVAEA 611

BLAST of Moc04g34620 vs. TAIR 10
Match: AT3G55400.1 (methionyl-tRNA synthetase / methionine--tRNA ligase / MetRS (cpMetRS) )

HSP 1 Score: 911.4 bits (2354), Expect = 4.0e-265
Identity = 461/624 (73.88%), Postives = 524/624 (83.97%), Query Frame = 0

Query: 1   MAARINSSIQSSLWLLNPLQSSLNLNLNLNLNLRTRLNFYRNLLPSPSYSA-SSSKRAIF 60
           MAARIN+S+ ++L  L P  + LN           R N +R     P YS  SS KRA++
Sbjct: 1   MAARINTSLHNALSFLKPFNTPLNTK-----PFSFRRNSFRFSKKLPYYSQFSSGKRALY 60

Query: 61  CTYASNCNSHESGAATDPFVITTPLYYVNAAPHMGSAYTTIAADAIARFQRLLGKKVIFI 120
           CT +S  ++ + G   + FV+TTPLYYVNA PHMGSAYTTIAAD+IARFQRLLGKKVIFI
Sbjct: 61  CTSSSQESTVDEG---ETFVLTTPLYYVNAPPHMGSAYTTIAADSIARFQRLLGKKVIFI 120

Query: 121 TGTDEHGEKIATAAAAQGSAPSEHCDAISEAYKTLWKDLDVAYDKFIRTTDPKHEAIVKE 180
           TGTDEHGEKIAT+AAA G  P EHCD IS++Y+TLWKDLD+AYDKFIRTTDPKHEAIVKE
Sbjct: 121 TGTDEHGEKIATSAAANGRNPPEHCDLISQSYRTLWKDLDIAYDKFIRTTDPKHEAIVKE 180

Query: 181 FYSKVLANGDIYRADYEGLYCVNCEEYKDEKELLANNCCPIHLKPCASRKEDNYFFALSK 240
           FY++V ANGDIYRADYEGLYCVNCEEYKDEKELL NNCCP+H  PC +RKEDNYFFALSK
Sbjct: 181 FYARVFANGDIYRADYEGLYCVNCEEYKDEKELLENNCCPVHQMPCVARKEDNYFFALSK 240

Query: 241 YQGLLEETLTQNPGFVQPAFRLNEVQSWIRSGLKDFSISRASVEWGIPVPNDQKQTIYVW 300
           YQ  LE+ L QNP FVQP++RLNEVQSWI+SGL+DFSISRA V+WGIPVP+D KQTIYVW
Sbjct: 241 YQKPLEDILAQNPRFVQPSYRLNEVQSWIKSGLRDFSISRALVDWGIPVPDDDKQTIYVW 300

Query: 301 FDALLGYISALSENGQQQNLQRAVASGWPASLHLIGKDILRFHAVYWPAMLMSAGLKLPK 360
           FDALLGYISAL+E+ +QQNL+ AV+ GWPASLHLIGKDILRFHAVYWPAMLMSAGL+LPK
Sbjct: 301 FDALLGYISALTEDNKQQNLETAVSFGWPASLHLIGKDILRFHAVYWPAMLMSAGLELPK 360

Query: 361 MVFGHGFLTKDGMKMGKSLGNTLEPNELVQKFGSDAVRYFFLQEVEFGSDGDYAEDRFIN 420
           MVFGHGFLTKDGMKMGKSLGNTLEP ELVQKFG DAVRYFFL+EVEFG+DGDY+EDRFI 
Sbjct: 361 MVFGHGFLTKDGMKMGKSLGNTLEPFELVQKFGPDAVRYFFLREVEFGNDGDYSEDRFIK 420

Query: 421 IVNAHLANTIGNLLNRTLGLLKKNCQSTLVVDSTAAAEGNSFKDKVEELVLKARNHYEGL 480
           IVNAHLANTIGNLLNRTLGLLKKNC+STLVVDST AAEG   KD VE+LV KAR +YE L
Sbjct: 421 IVNAHLANTIGNLLNRTLGLLKKNCESTLVVDSTVAAEGVPLKDTVEKLVEKARTNYENL 480

Query: 481 SLSSACEAVLEIGNAGNSYMDEQAPWSLFKQGGASSDAAAKDLVLILEAMRIIAVALSPV 540
           SLSSACEAVLEIGNAGN+YMD++APW LFKQGG S++ AAKDLV+ILE MR+IAVALSPV
Sbjct: 481 SLSSACEAVLEIGNAGNTYMDQRAPWFLFKQGGVSAEEAAKDLVIILEVMRVIAVALSPV 540

Query: 541 TPCLGWRIYAQLGFSKDQFEAATWNETKWGGLKAGQVMAPPNPVFARIEQSTGSDEDE-- 600
            PCL  RIY+QLG+++DQF + TW++TKWGGLK GQVM   +PVFARIE +   +EDE  
Sbjct: 541 APCLSLRIYSQLGYTEDQFNSITWSDTKWGGLKGGQVMEQASPVFARIELNPEKEEDEKK 600

Query: 601 --VPKKPVKSKKKIAQ---GVAEA 617
             V KK  K+K K+ +    VAEA
Sbjct: 601 PKVGKKTGKAKVKVVEQTPTVAEA 616

BLAST of Moc04g34620 vs. TAIR 10
Match: AT3G55400.2 (methionyl-tRNA synthetase / methionine--tRNA ligase / MetRS (cpMetRS) )

HSP 1 Score: 803.1 bits (2073), Expect = 1.5e-232
Identity = 401/523 (76.67%), Postives = 449/523 (85.85%), Query Frame = 0

Query: 1   MAARINSSIQSSLWLLNPLQSSLNLNLNLNLNLRTRLNFYRNLLPSPSYSA-SSSKRAIF 60
           MAARIN+S+ ++L  L P  + LN           R N +R     P YS  SS KRA++
Sbjct: 1   MAARINTSLHNALSFLKPFNTPLNTK-----PFSFRRNSFRFSKKLPYYSQFSSGKRALY 60

Query: 61  CTYASNCNSHESGAATDPFVITTPLYYVNAAPHMGSAYTTIAADAIARFQRLLGKKVIFI 120
           CT +S  ++ + G   + FV+TTPLYYVNA PHMGSAYTTIAAD+IARFQRLLGKKVIFI
Sbjct: 61  CTSSSQESTVDEG---ETFVLTTPLYYVNAPPHMGSAYTTIAADSIARFQRLLGKKVIFI 120

Query: 121 TGTDEHGEKIATAAAAQGSAPSEHCDAISEAYKTLWKDLDVAYDKFIRTTDPKHEAIVKE 180
           TGTDEHGEKIAT+AAA G  P EHCD IS++Y+TLWKDLD+AYDKFIRTTDPKHEAIVKE
Sbjct: 121 TGTDEHGEKIATSAAANGRNPPEHCDLISQSYRTLWKDLDIAYDKFIRTTDPKHEAIVKE 180

Query: 181 FYSKVLANGDIYRADYEGLYCVNCEEYKDEKELLANNCCPIHLKPCASRKEDNYFFALSK 240
           FY++V ANGDIYRADYEGLYCVNCEEYKDEKELL NNCCP+H  PC +RKEDNYFFALSK
Sbjct: 181 FYARVFANGDIYRADYEGLYCVNCEEYKDEKELLENNCCPVHQMPCVARKEDNYFFALSK 240

Query: 241 YQGLLEETLTQNPGFVQPAFRLNEVQSWIRSGLKDFSISRASVEWGIPVPNDQKQTIYVW 300
           YQ  LE+ L QNP FVQP++RLNEVQSWI+SGL+DFSISRA V+WGIPVP+D KQTIYVW
Sbjct: 241 YQKPLEDILAQNPRFVQPSYRLNEVQSWIKSGLRDFSISRALVDWGIPVPDDDKQTIYVW 300

Query: 301 FDALLGYISALSENGQQQNLQRAVASGWPASLHLIGKDILRFHAVYWPAMLMSAGLKLPK 360
           FDALLGYISAL+E+ +QQNL+ AV+ GWPASLHLIGKDILRFHAVYWPAMLMSAGL+LPK
Sbjct: 301 FDALLGYISALTEDNKQQNLETAVSFGWPASLHLIGKDILRFHAVYWPAMLMSAGLELPK 360

Query: 361 MVFGHGFLTKDGMKMGKSLGNTLEPNELVQKFGSDAVRYFFLQEVEFGSDGDYAEDRFIN 420
           MVFGHGFLTKDGMKMGKSLGNTLEP ELVQKFG DAVRYFFL+EVEFG+DGDY+EDRFI 
Sbjct: 361 MVFGHGFLTKDGMKMGKSLGNTLEPFELVQKFGPDAVRYFFLREVEFGNDGDYSEDRFIK 420

Query: 421 IVNAHLANTIGNLLNRTLGLLKKNCQSTLVVDSTAAAEGNSFKDKVEELVLKARNHYEGL 480
           IVNAHLANTIGNLLNRTLGLLKKNC+STLVVDST AAEG   KD VE+LV KAR +YE L
Sbjct: 421 IVNAHLANTIGNLLNRTLGLLKKNCESTLVVDSTVAAEGVPLKDTVEKLVEKARTNYENL 480

Query: 481 SLSSACEAVLEIGNAGNSYMDEQAPWSLFKQGGASSDAAAKDL 523
           SLSSACEAVLEIGNAGN+YMD++APW LFKQGG S++ AAK L
Sbjct: 481 SLSSACEAVLEIGNAGNTYMDQRAPWFLFKQGGVSAEEAAKVL 515

BLAST of Moc04g34620 vs. TAIR 10
Match: AT4G13780.1 (methionine--tRNA ligase, putative / methionyl-tRNA synthetase, putative / MetRS, putative )

HSP 1 Score: 162.2 bits (409), Expect = 1.4e-39
Identity = 143/571 (25.04%), Postives = 238/571 (41.68%), Query Frame = 0

Query: 79  VITTPLYYVNAAPHMGSAY-TTIAADAIARFQRLLGKKVIFITGTDEHGEKIATAAAAQG 138
           +IT+ L YVN  PH+G+     ++AD  AR+ RL G   I+I GTDE+G    T A  + 
Sbjct: 20  LITSALPYVNNVPHLGNIIGCVLSADVYARYCRLRGYNAIYICGTDEYGTATETKALEEN 79

Query: 139 SAPSEHCDAISEAYKTLWKDLDVAYDKFIRTTDPKHEAIVKEFYSKVLANGDIYRADYEG 198
             P E CD     +K ++    +++DKF RT+ P+   + +  ++K+  N  +     + 
Sbjct: 80  CTPKEICDKYHAIHKEVYDWFGISFDKFGRTSTPEQTEVCQAIFNKLWDNKWLSENTMQQ 139

Query: 199 LYCVNCEEYKDEKELLANNCCPIH----------------------------LKPCAS-- 258
           LYC  C+++  ++  L    CP                               K C +  
Sbjct: 140 LYCDTCKKFLADR--LVEGSCPFEGCNYDSARGDQCEKCGKLLNPTELKDPKCKVCQNTP 199

Query: 259 --RKEDNYFFALSKYQGLLEETLTQNPGFVQPAFRLNEVQ---SWIRSGLKDFSISRASV 318
             R  D+ F  L   +  LE  + +    V  ++  N +Q   +W+R GL+   I+R  +
Sbjct: 200 RIRDTDHLFIELPLLKDRLEAYIKKTS--VTGSWSQNAIQTTNAWLRDGLRQRCITR-DL 259

Query: 319 EWGIPVPND--QKQTIYVWFDALLGYISALSENGQQQNLQRAVASGW------PASLHL- 378
           +WG+PVP++  + +  YVWFDA +GY+S  S             S W      P ++ L 
Sbjct: 260 KWGVPVPHEKYKDKVFYVWFDAPIGYVSITS----------CYTSEWEKWWKNPENVELY 319

Query: 379 --IGKDILRFHAVYWPAMLMSA--GLKLPKMVFGHGFLTKDGMKMGKSLGNTLEPNELVQ 438
             +GKD + FH V +P+  +       L K +    +L  +  K  KS G  +  N++  
Sbjct: 320 QFMGKDNVPFHTVMFPSTQLGTEENWTLMKTISVTEYLNYEDGKFSKSKGVGVFGNDVKD 379

Query: 439 -KFGSDAVRYFFLQEVEFGSDGDYAEDRFINIVNAHLANTIGNLLNRTLGLLKK--NCQS 498
                +  RY+ L      SD  ++       +N  L + +GN +NR L  + K  N   
Sbjct: 380 TNIPVEVWRYYLLTNRPEVSDTSFSWTDLQAKLNGELLSNLGNFVNRVLSFIAKPDNAGY 439

Query: 499 TLVVDSTAAAEGN----SFKDKVEELVLKARNHYEGLSLSSACEAVLEIGNAGNSYMDEQ 558
             V+     AE +    S  +KVE+ V +     E + L    +  + I + GN Y+   
Sbjct: 440 GSVIPDAHDAESHSLTKSLAEKVEKFVAEYVEAMEKVKLKQGLKTAMLISSEGNYYLQAS 499

Query: 559 APWSLFKQGGASSDAAAKDLVLILEAMRIIAVALSPVTPCLGWRIYAQLG----FS-KDQ 589
             W L+K+        A  +      + ++A  L P  P     ++ QL     FS  D+
Sbjct: 500 QFWKLYKE---DKPLCAVVIRTAAGLVHLLAQLLEPFMPSFSCEVFKQLNLPPQFSLSDE 559

BLAST of Moc04g34620 vs. TAIR 10
Match: AT5G16715.1 (ATP binding;valine-tRNA ligases;aminoacyl-tRNA ligases;nucleotide binding;ATP binding;aminoacyl-tRNA ligases )

HSP 1 Score: 48.9 bits (115), Expect = 1.7e-05
Identity = 33/106 (31.13%), Postives = 52/106 (49.06%), Query Frame = 0

Query: 299 WFDALLGYISALSENGQQQNLQRAVASGWPASLHLIGKDILRFHAVYWPAMLMSAGLKLP 358
           WF + L   S L   G      +   + +P ++   G DIL F    W A ++  G++  
Sbjct: 525 WFSSSLWPFSTL---GWPDVAAKDFNNFYPTNMLETGHDILFF----WVARMVMMGIEFT 584

Query: 359 KMV-FGHGFL-----TKDGMKMGKSLGNTLEPNELVQKFGSDAVRY 399
             V F H +L        G KM KSLGN ++P + ++ FG+DA+R+
Sbjct: 585 GTVPFSHVYLHGLIRDSQGRKMSKSLGNVIDPLDTIKDFGTDALRF 623

BLAST of Moc04g34620 vs. TAIR 10
Match: AT5G49030.1 (tRNA synthetase class I (I, L, M and V) family protein )

HSP 1 Score: 47.8 bits (112), Expect = 3.8e-05
Identity = 78/306 (25.49%), Postives = 123/306 (40.20%), Query Frame = 0

Query: 295 TIYVWFDALLGYISALSENGQQQNLQRAVASGWPASLHLIGKDILRFHAVYWPAMLMSA- 354
           T+ VWFD+   +   L   G+++ L       +PA ++L G D    H  ++ + L+++ 
Sbjct: 652 TMDVWFDSGSSWAGVL---GKREGL------SFPADVYLEGTD---QHRGWFQSSLLTSI 711

Query: 355 ---GLKLPKMVFGHGF-LTKDGMKMGKSLGNTLEPNELVQ--KFGSDAVRYFFLQEVEFG 414
              G      V  HGF L + GMKM KSLGN ++P  +++  K   DA  Y       + 
Sbjct: 712 ATQGKAPYSAVITHGFVLDEKGMKMSKSLGNVVDPRLVIEGGKNSKDAPAYGADVMRLWV 771

Query: 415 SDGDYAEDRFINIVNAHLANTIGNLLNRTLGLLKKNCQSTLVVDSTAAAEGNSFKD---- 474
           S  DY  D  I        + I   L  TL  L  N      VD+    +     D    
Sbjct: 772 SSVDYTGDVLIGPQILRQMSDIYRKLRGTLRYLLGNLHDWR-VDNAVPYQDLPIIDQHAL 831

Query: 475 -KVEELVLKARNHYEGLSLSSACEAV--LEIGNAGNSYMDEQAPWSLFKQGGASSDAAAK 534
            ++E +V   +  YE        + +    I +  N Y D  A   L+  G +S    + 
Sbjct: 832 FQLENVVKNIQECYENYQFFKIFQIIQRFTIVDLSNFYFD-IAKDRLYTGGTSSFTRRSC 891

Query: 535 DLVLILEAMRIIAVALSPVTPCLGWRIYAQLGF---SKDQFEAATWNETKWGGLKAGQVM 584
             VL    + I+ V ++P+ P L   ++  L F   ++D   A    E KW  L    + 
Sbjct: 892 QTVLSTHLLSILRV-IAPIVPHLAEDVWQNLPFEYRNEDGSAAEFVFELKWPTLNEQWLS 942

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022133974.10.0e+00100.00methionine--tRNA ligase, chloroplastic/mitochondrial [Momordica charantia][more]
XP_038891037.10.0e+0090.42methionine--tRNA ligase, chloroplastic/mitochondrial [Benincasa hispida][more]
XP_004142742.10.0e+0090.42methionine--tRNA ligase, chloroplastic/mitochondrial [Cucumis sativus] >KGN51284... [more]
KAA0066020.10.0e+0090.42methionine--tRNA ligase [Cucumis melo var. makuwa] >TYK05823.1 methionine--tRNA ... [more]
XP_022960433.10.0e+0090.44methionine--tRNA ligase, chloroplastic/mitochondrial [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
Q9M2T95.6e-26473.88Methionine--tRNA ligase, chloroplastic/mitochondrial OS=Arabidopsis thaliana OX=... [more]
Q557291.9e-15852.98Methionine--tRNA ligase OS=Synechocystis sp. (strain PCC 6803 / Kazusa) OX=11117... [more]
Q8Z0683.5e-15752.96Methionine--tRNA ligase OS=Nostoc sp. (strain PCC 7120 / SAG 25.82 / UTEX 2576) ... [more]
P590811.6e-14950.77Methionine--tRNA ligase OS=Thermosynechococcus elongatus (strain BP-1) OX=197221... [more]
O672981.3e-11946.57Methionine--tRNA ligase OS=Aquifex aeolicus (strain VF5) OX=224324 GN=metG PE=1 ... [more]
Match NameE-valueIdentityDescription
A0A6J1BXG80.0e+00100.00Methionine--tRNA ligase OS=Momordica charantia OX=3673 GN=LOC111006379 PE=3 SV=1[more]
A0A0A0KQV70.0e+0090.42Methionine--tRNA ligase OS=Cucumis sativus OX=3659 GN=Csa_5G512910 PE=3 SV=1[more]
A0A5D3C1K00.0e+0090.42Methionine--tRNA ligase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold... [more]
A0A6J1H8X30.0e+0090.44Methionine--tRNA ligase OS=Cucurbita moschata OX=3662 GN=LOC111461165 PE=3 SV=1[more]
A0A1S3CJS70.0e+0090.42Methionine--tRNA ligase OS=Cucumis melo OX=3656 GN=LOC103501636 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT3G55400.14.0e-26573.88methionyl-tRNA synthetase / methionine--tRNA ligase / MetRS (cpMetRS) [more]
AT3G55400.21.5e-23276.67methionyl-tRNA synthetase / methionine--tRNA ligase / MetRS (cpMetRS) [more]
AT4G13780.11.4e-3925.04methionine--tRNA ligase, putative / methionyl-tRNA synthetase, putative / MetRS,... [more]
AT5G16715.11.7e-0531.13ATP binding;valine-tRNA ligases;aminoacyl-tRNA ligases;nucleotide binding;ATP bi... [more]
AT5G49030.13.8e-0525.49tRNA synthetase class I (I, L, M and V) family protein [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (OHB3-1) v2
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR033911Methioninyl-tRNA synthetase core domainPRINTSPR01041TRNASYNTHMETcoord: 424..435
score: 51.85
coord: 112..126
score: 54.81
coord: 296..307
score: 72.53
coord: 80..93
score: 56.61
coord: 332..347
score: 65.28
coord: 160..171
score: 51.54
IPR033911Methioninyl-tRNA synthetase core domainCDDcd00814MetRS_corecoord: 77..412
e-value: 3.47071E-163
score: 467.394
IPR014729Rossmann-like alpha/beta/alpha sandwich foldGENE3D3.40.50.620HUPscoord: 78..409
e-value: 8.6E-140
score: 467.5
NoneNo IPR availableGENE3D1.10.730.10coord: 413..592
e-value: 2.2E-41
score: 143.3
NoneNo IPR availableGENE3D2.170.220.10coord: 189..298
e-value: 8.6E-140
score: 467.5
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 588..616
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 590..616
NoneNo IPR availablePANTHERPTHR43326:SF6BNAA09G34980D PROTEINcoord: 1..609
NoneNo IPR availablePANTHERPTHR43326METHIONYL-TRNA SYNTHETASEcoord: 1..609
NoneNo IPR availableSUPERFAMILY52374Nucleotidylyl transferasecoord: 76..422
IPR014758Methionyl-tRNA synthetaseTIGRFAMTIGR00398TIGR00398coord: 79..587
e-value: 2.4E-155
score: 516.2
IPR015413Methionyl/Leucyl tRNA synthetasePFAMPF09334tRNA-synt_1gcoord: 212..437
e-value: 3.2E-62
score: 210.5
IPR002300Aminoacyl-tRNA synthetase, class IaPFAMPF00133tRNA-synt_1coord: 71..203
e-value: 2.0E-8
score: 33.0
IPR023457Methionine-tRNA synthetase, type 2HAMAPMF_01228Met_tRNA_synth_type2coord: 69..583
score: 42.457775
IPR041872Methionyl-tRNA synthetase, anticodon-binding domainCDDcd07957Anticodon_Ia_Metcoord: 421..551
e-value: 1.7002E-34
score: 125.295
IPR009080Aminoacyl-tRNA synthetase, class Ia, anticodon-bindingSUPERFAMILY47323Anticodon-binding domain of a subclass of class I aminoacyl-tRNA synthetasescoord: 425..587

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Moc04g34620.1Moc04g34620.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006431 methionyl-tRNA aminoacylation
biological_process GO:0006418 tRNA aminoacylation for protein translation
cellular_component GO:0005739 mitochondrion
molecular_function GO:0005524 ATP binding
molecular_function GO:0004825 methionine-tRNA ligase activity
molecular_function GO:0004812 aminoacyl-tRNA ligase activity
molecular_function GO:0000166 nucleotide binding