Homology
BLAST of Moc04g34620 vs. NCBI nr
Match:
XP_022133974.1 (methionine--tRNA ligase, chloroplastic/mitochondrial [Momordica charantia])
HSP 1 Score: 1232.2 bits (3187), Expect = 0.0e+00
Identity = 616/616 (100.00%), Postives = 616/616 (100.00%), Query Frame = 0
Query: 1 MAARINSSIQSSLWLLNPLQSSLNLNLNLNLNLRTRLNFYRNLLPSPSYSASSSKRAIFC 60
MAARINSSIQSSLWLLNPLQSSLNLNLNLNLNLRTRLNFYRNLLPSPSYSASSSKRAIFC
Sbjct: 1 MAARINSSIQSSLWLLNPLQSSLNLNLNLNLNLRTRLNFYRNLLPSPSYSASSSKRAIFC 60
Query: 61 TYASNCNSHESGAATDPFVITTPLYYVNAAPHMGSAYTTIAADAIARFQRLLGKKVIFIT 120
TYASNCNSHESGAATDPFVITTPLYYVNAAPHMGSAYTTIAADAIARFQRLLGKKVIFIT
Sbjct: 61 TYASNCNSHESGAATDPFVITTPLYYVNAAPHMGSAYTTIAADAIARFQRLLGKKVIFIT 120
Query: 121 GTDEHGEKIATAAAAQGSAPSEHCDAISEAYKTLWKDLDVAYDKFIRTTDPKHEAIVKEF 180
GTDEHGEKIATAAAAQGSAPSEHCDAISEAYKTLWKDLDVAYDKFIRTTDPKHEAIVKEF
Sbjct: 121 GTDEHGEKIATAAAAQGSAPSEHCDAISEAYKTLWKDLDVAYDKFIRTTDPKHEAIVKEF 180
Query: 181 YSKVLANGDIYRADYEGLYCVNCEEYKDEKELLANNCCPIHLKPCASRKEDNYFFALSKY 240
YSKVLANGDIYRADYEGLYCVNCEEYKDEKELLANNCCPIHLKPCASRKEDNYFFALSKY
Sbjct: 181 YSKVLANGDIYRADYEGLYCVNCEEYKDEKELLANNCCPIHLKPCASRKEDNYFFALSKY 240
Query: 241 QGLLEETLTQNPGFVQPAFRLNEVQSWIRSGLKDFSISRASVEWGIPVPNDQKQTIYVWF 300
QGLLEETLTQNPGFVQPAFRLNEVQSWIRSGLKDFSISRASVEWGIPVPNDQKQTIYVWF
Sbjct: 241 QGLLEETLTQNPGFVQPAFRLNEVQSWIRSGLKDFSISRASVEWGIPVPNDQKQTIYVWF 300
Query: 301 DALLGYISALSENGQQQNLQRAVASGWPASLHLIGKDILRFHAVYWPAMLMSAGLKLPKM 360
DALLGYISALSENGQQQNLQRAVASGWPASLHLIGKDILRFHAVYWPAMLMSAGLKLPKM
Sbjct: 301 DALLGYISALSENGQQQNLQRAVASGWPASLHLIGKDILRFHAVYWPAMLMSAGLKLPKM 360
Query: 361 VFGHGFLTKDGMKMGKSLGNTLEPNELVQKFGSDAVRYFFLQEVEFGSDGDYAEDRFINI 420
VFGHGFLTKDGMKMGKSLGNTLEPNELVQKFGSDAVRYFFLQEVEFGSDGDYAEDRFINI
Sbjct: 361 VFGHGFLTKDGMKMGKSLGNTLEPNELVQKFGSDAVRYFFLQEVEFGSDGDYAEDRFINI 420
Query: 421 VNAHLANTIGNLLNRTLGLLKKNCQSTLVVDSTAAAEGNSFKDKVEELVLKARNHYEGLS 480
VNAHLANTIGNLLNRTLGLLKKNCQSTLVVDSTAAAEGNSFKDKVEELVLKARNHYEGLS
Sbjct: 421 VNAHLANTIGNLLNRTLGLLKKNCQSTLVVDSTAAAEGNSFKDKVEELVLKARNHYEGLS 480
Query: 481 LSSACEAVLEIGNAGNSYMDEQAPWSLFKQGGASSDAAAKDLVLILEAMRIIAVALSPVT 540
LSSACEAVLEIGNAGNSYMDEQAPWSLFKQGGASSDAAAKDLVLILEAMRIIAVALSPVT
Sbjct: 481 LSSACEAVLEIGNAGNSYMDEQAPWSLFKQGGASSDAAAKDLVLILEAMRIIAVALSPVT 540
Query: 541 PCLGWRIYAQLGFSKDQFEAATWNETKWGGLKAGQVMAPPNPVFARIEQSTGSDEDEVPK 600
PCLGWRIYAQLGFSKDQFEAATWNETKWGGLKAGQVMAPPNPVFARIEQSTGSDEDEVPK
Sbjct: 541 PCLGWRIYAQLGFSKDQFEAATWNETKWGGLKAGQVMAPPNPVFARIEQSTGSDEDEVPK 600
Query: 601 KPVKSKKKIAQGVAEA 617
KPVKSKKKIAQGVAEA
Sbjct: 601 KPVKSKKKIAQGVAEA 616
BLAST of Moc04g34620 vs. NCBI nr
Match:
XP_038891037.1 (methionine--tRNA ligase, chloroplastic/mitochondrial [Benincasa hispida])
HSP 1 Score: 1112.8 bits (2877), Expect = 0.0e+00
Identity = 557/616 (90.42%), Postives = 589/616 (95.62%), Query Frame = 0
Query: 1 MAARINSSIQSSLWLLNPLQSSLNLNLNLNLNLRTRLNFYRNLLPSPSYSASSSKRAIFC 60
MAARIN+SIQ+SLWLLNP QSSLNL +LRTRL+FY+NLL PS S+SSSKRAIFC
Sbjct: 1 MAARINTSIQTSLWLLNPCQSSLNLK---PTHLRTRLHFYQNLLSLPS-SSSSSKRAIFC 60
Query: 61 TYASNCNSHESGAATDPFVITTPLYYVNAAPHMGSAYTTIAADAIARFQRLLGKKVIFIT 120
T A++ NSH++GAAT+PFVITTPLYYVNAAPHMGSAYTTIAADAIARFQRLLGKKVIFIT
Sbjct: 61 TAATSFNSHDNGAATNPFVITTPLYYVNAAPHMGSAYTTIAADAIARFQRLLGKKVIFIT 120
Query: 121 GTDEHGEKIATAAAAQGSAPSEHCDAISEAYKTLWKDLDVAYDKFIRTTDPKHEAIVKEF 180
GTDEHGEKIATAAA QGS+PSEHCD ISEAYKTLWKDLDVAYD+FIRTTDPKHEAIVKEF
Sbjct: 121 GTDEHGEKIATAAATQGSSPSEHCDVISEAYKTLWKDLDVAYDRFIRTTDPKHEAIVKEF 180
Query: 181 YSKVLANGDIYRADYEGLYCVNCEEYKDEKELLANNCCPIHLKPCASRKEDNYFFALSKY 240
YS+VLANGDIYRADYEGLYCVNCEEYKDEKELLA+NCCPIHLKPCASR+EDNYFFALSKY
Sbjct: 181 YSRVLANGDIYRADYEGLYCVNCEEYKDEKELLADNCCPIHLKPCASRREDNYFFALSKY 240
Query: 241 QGLLEETLTQNPGFVQPAFRLNEVQSWIRSGLKDFSISRASVEWGIPVPNDQKQTIYVWF 300
Q LLEETLTQNPGFVQPAFRLNEVQSWIRSGLKDFSISRASVEWGIPVPND+KQTIYVWF
Sbjct: 241 QRLLEETLTQNPGFVQPAFRLNEVQSWIRSGLKDFSISRASVEWGIPVPNDEKQTIYVWF 300
Query: 301 DALLGYISALSENGQQQNLQRAVASGWPASLHLIGKDILRFHAVYWPAMLMSAGLKLPKM 360
DALLGYISALSENG+Q NL+R VASGWPASLHLIGKDILRFHAVYWPAMLMSAGLKLPKM
Sbjct: 301 DALLGYISALSENGEQPNLERVVASGWPASLHLIGKDILRFHAVYWPAMLMSAGLKLPKM 360
Query: 361 VFGHGFLTKDGMKMGKSLGNTLEPNELVQKFGSDAVRYFFLQEVEFGSDGDYAEDRFINI 420
VFGHGFLTKDGMKMGKSLGNTLEPNELV KFGSDAVRYFFL+EVEFGSDGDYAEDRFINI
Sbjct: 361 VFGHGFLTKDGMKMGKSLGNTLEPNELVGKFGSDAVRYFFLREVEFGSDGDYAEDRFINI 420
Query: 421 VNAHLANTIGNLLNRTLGLLKKNCQSTLVVDSTAAAEGNSFKDKVEELVLKARNHYEGLS 480
VNAHLANTIGNLLNRTLGLL+KNCQSTLVVDSTAAA GN FKDKVEEL++KARNHYE L+
Sbjct: 421 VNAHLANTIGNLLNRTLGLLQKNCQSTLVVDSTAAAGGNVFKDKVEELIVKARNHYEELA 480
Query: 481 LSSACEAVLEIGNAGNSYMDEQAPWSLFKQGGASSDAAAKDLVLILEAMRIIAVALSPVT 540
LSSACEAVLEIGNAGNSYMDE+APWSLFKQGGAS+DAAAKDLVLILEAMRIIA+ALSPVT
Sbjct: 481 LSSACEAVLEIGNAGNSYMDERAPWSLFKQGGASTDAAAKDLVLILEAMRIIAIALSPVT 540
Query: 541 PCLGWRIYAQLGFSKDQFEAATWNETKWGGLKAGQVMAPPNPVFARIEQSTGSDEDEVPK 600
P L WRIYAQLG+S+DQF AATWNETKWGGLKAGQVMAPPNPVFARIEQ+ + E+EVPK
Sbjct: 541 PRLSWRIYAQLGYSEDQFNAATWNETKWGGLKAGQVMAPPNPVFARIEQAIVNGEEEVPK 600
Query: 601 KPVKSKKKIAQGVAEA 617
KPVKSKKKIAQGVAEA
Sbjct: 601 KPVKSKKKIAQGVAEA 612
BLAST of Moc04g34620 vs. NCBI nr
Match:
XP_004142742.1 (methionine--tRNA ligase, chloroplastic/mitochondrial [Cucumis sativus] >KGN51284.1 hypothetical protein Csa_008274 [Cucumis sativus])
HSP 1 Score: 1110.9 bits (2872), Expect = 0.0e+00
Identity = 557/616 (90.42%), Postives = 584/616 (94.81%), Query Frame = 0
Query: 1 MAARINSSIQSSLWLLNPLQSSLNLNLNLNLNLRTRLNFYRNLLPSPSYSASSSKRAIFC 60
MAARIN+SIQ+SLWLLNP SSLNL + RTRL+F +NLL PS+ SSSKRAIFC
Sbjct: 1 MAARINTSIQTSLWLLNPFHSSLNLK---PTHFRTRLHFCQNLLSFPSH--SSSKRAIFC 60
Query: 61 TYASNCNSHESGAATDPFVITTPLYYVNAAPHMGSAYTTIAADAIARFQRLLGKKVIFIT 120
T SN NSH+SGAATDPFVITTPLYYVNAAPHMGSAYTTIAADA+ARFQRLLGKKVIFIT
Sbjct: 61 TSTSNFNSHDSGAATDPFVITTPLYYVNAAPHMGSAYTTIAADAVARFQRLLGKKVIFIT 120
Query: 121 GTDEHGEKIATAAAAQGSAPSEHCDAISEAYKTLWKDLDVAYDKFIRTTDPKHEAIVKEF 180
GTDEHGEKIATAAA QGS PSEHCD ISEAYKTLWKDLDVAYDKFIRTTDPKHEAIVKEF
Sbjct: 121 GTDEHGEKIATAAATQGSTPSEHCDIISEAYKTLWKDLDVAYDKFIRTTDPKHEAIVKEF 180
Query: 181 YSKVLANGDIYRADYEGLYCVNCEEYKDEKELLANNCCPIHLKPCASRKEDNYFFALSKY 240
YSKVLANGDIYRADYEGLYCVNCEEYKDEKELLA+NCCPIHLKPCASRKEDNYFFALSKY
Sbjct: 181 YSKVLANGDIYRADYEGLYCVNCEEYKDEKELLADNCCPIHLKPCASRKEDNYFFALSKY 240
Query: 241 QGLLEETLTQNPGFVQPAFRLNEVQSWIRSGLKDFSISRASVEWGIPVPNDQKQTIYVWF 300
Q LLEETLTQNPGFVQPAFRLNEVQSWIRSGLKDFSISRASVEWGIPVPND+KQTIYVWF
Sbjct: 241 QRLLEETLTQNPGFVQPAFRLNEVQSWIRSGLKDFSISRASVEWGIPVPNDEKQTIYVWF 300
Query: 301 DALLGYISALSENGQQQNLQRAVASGWPASLHLIGKDILRFHAVYWPAMLMSAGLKLPKM 360
DALLGYISALSENG+Q NLQ VASGWPASLHLIGKDILRFHAVYWPAMLMSAGLKLPKM
Sbjct: 301 DALLGYISALSENGEQPNLQEVVASGWPASLHLIGKDILRFHAVYWPAMLMSAGLKLPKM 360
Query: 361 VFGHGFLTKDGMKMGKSLGNTLEPNELVQKFGSDAVRYFFLQEVEFGSDGDYAEDRFINI 420
VFGHGFLTKDGMKMGKSLGNTLEPNELV+KFGSDAVRYFFL+EVEFGSDGDYAEDRFINI
Sbjct: 361 VFGHGFLTKDGMKMGKSLGNTLEPNELVEKFGSDAVRYFFLREVEFGSDGDYAEDRFINI 420
Query: 421 VNAHLANTIGNLLNRTLGLLKKNCQSTLVVDSTAAAEGNSFKDKVEELVLKARNHYEGLS 480
VNAHLANTIGNLLNRTLGLLKKNCQSTLVVDSTAAA+GN+FKDKVEELV+KARN+YE L+
Sbjct: 421 VNAHLANTIGNLLNRTLGLLKKNCQSTLVVDSTAAADGNAFKDKVEELVVKARNNYEELA 480
Query: 481 LSSACEAVLEIGNAGNSYMDEQAPWSLFKQGGASSDAAAKDLVLILEAMRIIAVALSPVT 540
LSSACEAVLEIGNAGNSYMDE+APWSLFKQGGASS+AAAKDLVLILEAMRIIA+ALSPVT
Sbjct: 481 LSSACEAVLEIGNAGNSYMDERAPWSLFKQGGASSNAAAKDLVLILEAMRIIAIALSPVT 540
Query: 541 PCLGWRIYAQLGFSKDQFEAATWNETKWGGLKAGQVMAPPNPVFARIEQSTGSDEDEVPK 600
P L WRIYAQLG+SKDQF+AATWNETKWGGLKAGQVMAPPNPVFARIEQ+ + E+E PK
Sbjct: 541 PRLSWRIYAQLGYSKDQFDAATWNETKWGGLKAGQVMAPPNPVFARIEQAIVNGEEEAPK 600
Query: 601 KPVKSKKKIAQGVAEA 617
KPVKSKKKIAQ VAE+
Sbjct: 601 KPVKSKKKIAQAVAES 611
BLAST of Moc04g34620 vs. NCBI nr
Match:
KAA0066020.1 (methionine--tRNA ligase [Cucumis melo var. makuwa] >TYK05823.1 methionine--tRNA ligase [Cucumis melo var. makuwa])
HSP 1 Score: 1108.6 bits (2866), Expect = 0.0e+00
Identity = 557/616 (90.42%), Postives = 584/616 (94.81%), Query Frame = 0
Query: 1 MAARINSSIQSSLWLLNPLQSSLNLNLNLNLNLRTRLNFYRNLLPSPSYSASSSKRAIFC 60
MAARIN+SIQ+SL LLNP +SLNL + RTRL+FY+NLL SY SSSKRAIFC
Sbjct: 1 MAARINTSIQTSLSLLNPFHNSLNLK---PTHFRTRLHFYQNLLSFASY--SSSKRAIFC 60
Query: 61 TYASNCNSHESGAATDPFVITTPLYYVNAAPHMGSAYTTIAADAIARFQRLLGKKVIFIT 120
T SN NSH++GAATDPFVITTPLYYVNAAPHMGSAYTTIAADAIARFQRLLGKKVI IT
Sbjct: 61 TTTSNFNSHDTGAATDPFVITTPLYYVNAAPHMGSAYTTIAADAIARFQRLLGKKVILIT 120
Query: 121 GTDEHGEKIATAAAAQGSAPSEHCDAISEAYKTLWKDLDVAYDKFIRTTDPKHEAIVKEF 180
GTDEHGEKIATAAA QGS PSEHCD ISEAYKTLWKDLDVAYDKFIRTTDPKHEAIVKEF
Sbjct: 121 GTDEHGEKIATAAATQGSTPSEHCDIISEAYKTLWKDLDVAYDKFIRTTDPKHEAIVKEF 180
Query: 181 YSKVLANGDIYRADYEGLYCVNCEEYKDEKELLANNCCPIHLKPCASRKEDNYFFALSKY 240
YSKVLANGDIYRADYEGLYCVNCEEYKDEKELLA+NCCPIHLKPCASRKEDNYFFALSKY
Sbjct: 181 YSKVLANGDIYRADYEGLYCVNCEEYKDEKELLADNCCPIHLKPCASRKEDNYFFALSKY 240
Query: 241 QGLLEETLTQNPGFVQPAFRLNEVQSWIRSGLKDFSISRASVEWGIPVPNDQKQTIYVWF 300
Q LLEETLTQNPGFVQPAFRLNEVQSWIRSGLKDFSISRASVEWGIPVPND+KQTIYVWF
Sbjct: 241 QRLLEETLTQNPGFVQPAFRLNEVQSWIRSGLKDFSISRASVEWGIPVPNDKKQTIYVWF 300
Query: 301 DALLGYISALSENGQQQNLQRAVASGWPASLHLIGKDILRFHAVYWPAMLMSAGLKLPKM 360
DALLGYISALSENG+Q NL + VASGWPASLHLIGKDILRFHAVYWPAMLMSAGLKLPKM
Sbjct: 301 DALLGYISALSENGEQPNLHKVVASGWPASLHLIGKDILRFHAVYWPAMLMSAGLKLPKM 360
Query: 361 VFGHGFLTKDGMKMGKSLGNTLEPNELVQKFGSDAVRYFFLQEVEFGSDGDYAEDRFINI 420
VFGHGFLTKDGMKMGKSLGNTLEPNELV+KFGSDAVRYFFL+EVEFGSDGDYAEDRFINI
Sbjct: 361 VFGHGFLTKDGMKMGKSLGNTLEPNELVEKFGSDAVRYFFLREVEFGSDGDYAEDRFINI 420
Query: 421 VNAHLANTIGNLLNRTLGLLKKNCQSTLVVDSTAAAEGNSFKDKVEELVLKARNHYEGLS 480
VNAHLANTIGNLLNRTLGLLKKNCQSTL+VDSTAAA+GN+FKDKVEELV+KARNHYE L+
Sbjct: 421 VNAHLANTIGNLLNRTLGLLKKNCQSTLLVDSTAAADGNAFKDKVEELVVKARNHYEELA 480
Query: 481 LSSACEAVLEIGNAGNSYMDEQAPWSLFKQGGASSDAAAKDLVLILEAMRIIAVALSPVT 540
LSSACEAVLEIGNAGNSYMDE+APWSLFKQGGASS+AAAKDLVLILEAMRIIA+ALSPVT
Sbjct: 481 LSSACEAVLEIGNAGNSYMDERAPWSLFKQGGASSNAAAKDLVLILEAMRIIAIALSPVT 540
Query: 541 PCLGWRIYAQLGFSKDQFEAATWNETKWGGLKAGQVMAPPNPVFARIEQSTGSDEDEVPK 600
P L WRIYAQLG+SKDQF+AATWNETKWGGLKAGQVMAPPNPVFARIEQ+ + E+EVPK
Sbjct: 541 PRLSWRIYAQLGYSKDQFDAATWNETKWGGLKAGQVMAPPNPVFARIEQAIVNGEEEVPK 600
Query: 601 KPVKSKKKIAQGVAEA 617
KPVKSKKKIAQGVAEA
Sbjct: 601 KPVKSKKKIAQGVAEA 611
BLAST of Moc04g34620 vs. NCBI nr
Match:
XP_022960433.1 (methionine--tRNA ligase, chloroplastic/mitochondrial [Cucurbita moschata])
HSP 1 Score: 1108.2 bits (2865), Expect = 0.0e+00
Identity = 558/617 (90.44%), Postives = 581/617 (94.17%), Query Frame = 0
Query: 1 MAARINSSIQSSLWLLNPLQSSLNLNLNLNLNLRTRLNFYRNLLPSP-SYSASSSKRAIF 60
MAARIN+SIQ+SLWLLNPLQSS NL +LRTR NFY+NLL +P S S+SSSKRAIF
Sbjct: 1 MAARINTSIQNSLWLLNPLQSSRNLKAT---HLRTRFNFYQNLLSAPSSCSSSSSKRAIF 60
Query: 61 CTYASNCNSHESGAATDPFVITTPLYYVNAAPHMGSAYTTIAADAIARFQRLLGKKVIFI 120
C+ ASN NSH+ GAATDPFVITTPLYYVNAAPHMGSAYTTIAADAIARFQRLLGKKVIFI
Sbjct: 61 CSSASNFNSHDGGAATDPFVITTPLYYVNAAPHMGSAYTTIAADAIARFQRLLGKKVIFI 120
Query: 121 TGTDEHGEKIATAAAAQGSAPSEHCDAISEAYKTLWKDLDVAYDKFIRTTDPKHEAIVKE 180
TGTDEHGEKIATAAAAQGS PSEHCD ISEAYKTLWKDLD+AYDKFIRTTDPKHE IVKE
Sbjct: 121 TGTDEHGEKIATAAAAQGSGPSEHCDNISEAYKTLWKDLDIAYDKFIRTTDPKHETIVKE 180
Query: 181 FYSKVLANGDIYRADYEGLYCVNCEEYKDEKELLANNCCPIHLKPCASRKEDNYFFALSK 240
FYS VLANGDIYRADYEGLYCVNCEEYKDEKELLA+NCCPIHLKPCASRKEDNYFFALSK
Sbjct: 181 FYSNVLANGDIYRADYEGLYCVNCEEYKDEKELLADNCCPIHLKPCASRKEDNYFFALSK 240
Query: 241 YQGLLEETLTQNPGFVQPAFRLNEVQSWIRSGLKDFSISRASVEWGIPVPNDQKQTIYVW 300
YQ LLEETLTQNPGFVQPAFRLNEVQSWI+SGLKDFSISRASVEWGIPVPNDQKQTIYVW
Sbjct: 241 YQRLLEETLTQNPGFVQPAFRLNEVQSWIKSGLKDFSISRASVEWGIPVPNDQKQTIYVW 300
Query: 301 FDALLGYISALSENGQQQNLQRAVASGWPASLHLIGKDILRFHAVYWPAMLMSAGLKLPK 360
FDALLGYISALSEN +Q NLQ AVASGWPASLHLIGKDILRFHAVYWPAMLMSAGLKLPK
Sbjct: 301 FDALLGYISALSENDEQPNLQTAVASGWPASLHLIGKDILRFHAVYWPAMLMSAGLKLPK 360
Query: 361 MVFGHGFLTKDGMKMGKSLGNTLEPNELVQKFGSDAVRYFFLQEVEFGSDGDYAEDRFIN 420
MVFGHGFLTKDGMKMGKSLGNTLEPNELVQKFGSD VRYFFL+EVEFGSDGDYAEDRFIN
Sbjct: 361 MVFGHGFLTKDGMKMGKSLGNTLEPNELVQKFGSDPVRYFFLREVEFGSDGDYAEDRFIN 420
Query: 421 IVNAHLANTIGNLLNRTLGLLKKNCQSTLVVDSTAAAEGNSFKDKVEELVLKARNHYEGL 480
IVNAHLANTIGNLLNRTLGLLKKNCQSTLVVDS+AAA GN+FK+KVEELV KARNHYE L
Sbjct: 421 IVNAHLANTIGNLLNRTLGLLKKNCQSTLVVDSSAAAVGNAFKEKVEELVAKARNHYEEL 480
Query: 481 SLSSACEAVLEIGNAGNSYMDEQAPWSLFKQGGASSDAAAKDLVLILEAMRIIAVALSPV 540
SLSSACEAVLEIGNAGNSYMDE+APWSLFKQGGASSDAAAKDLVLILEAMRIIA+ALSPV
Sbjct: 481 SLSSACEAVLEIGNAGNSYMDERAPWSLFKQGGASSDAAAKDLVLILEAMRIIAIALSPV 540
Query: 541 TPCLGWRIYAQLGFSKDQFEAATWNETKWGGLKAGQVMAPPNPVFARIEQSTGSDEDEVP 600
TP L WRIY QLG+ +DQF AATWNETKWGGLKAGQVMAPPNPVFARIEQ+ + E+E P
Sbjct: 541 TPRLSWRIYEQLGYPEDQFAAATWNETKWGGLKAGQVMAPPNPVFARIEQAIVNGEEEAP 600
Query: 601 KKPVKSKKKIAQGVAEA 617
KKPVKSKKKIAQ VAEA
Sbjct: 601 KKPVKSKKKIAQKVAEA 614
BLAST of Moc04g34620 vs. ExPASy Swiss-Prot
Match:
Q9M2T9 (Methionine--tRNA ligase, chloroplastic/mitochondrial OS=Arabidopsis thaliana OX=3702 GN=OVA1 PE=2 SV=1)
HSP 1 Score: 911.4 bits (2354), Expect = 5.6e-264
Identity = 461/624 (73.88%), Postives = 524/624 (83.97%), Query Frame = 0
Query: 1 MAARINSSIQSSLWLLNPLQSSLNLNLNLNLNLRTRLNFYRNLLPSPSYSA-SSSKRAIF 60
MAARIN+S+ ++L L P + LN R N +R P YS SS KRA++
Sbjct: 1 MAARINTSLHNALSFLKPFNTPLNTK-----PFSFRRNSFRFSKKLPYYSQFSSGKRALY 60
Query: 61 CTYASNCNSHESGAATDPFVITTPLYYVNAAPHMGSAYTTIAADAIARFQRLLGKKVIFI 120
CT +S ++ + G + FV+TTPLYYVNA PHMGSAYTTIAAD+IARFQRLLGKKVIFI
Sbjct: 61 CTSSSQESTVDEG---ETFVLTTPLYYVNAPPHMGSAYTTIAADSIARFQRLLGKKVIFI 120
Query: 121 TGTDEHGEKIATAAAAQGSAPSEHCDAISEAYKTLWKDLDVAYDKFIRTTDPKHEAIVKE 180
TGTDEHGEKIAT+AAA G P EHCD IS++Y+TLWKDLD+AYDKFIRTTDPKHEAIVKE
Sbjct: 121 TGTDEHGEKIATSAAANGRNPPEHCDLISQSYRTLWKDLDIAYDKFIRTTDPKHEAIVKE 180
Query: 181 FYSKVLANGDIYRADYEGLYCVNCEEYKDEKELLANNCCPIHLKPCASRKEDNYFFALSK 240
FY++V ANGDIYRADYEGLYCVNCEEYKDEKELL NNCCP+H PC +RKEDNYFFALSK
Sbjct: 181 FYARVFANGDIYRADYEGLYCVNCEEYKDEKELLENNCCPVHQMPCVARKEDNYFFALSK 240
Query: 241 YQGLLEETLTQNPGFVQPAFRLNEVQSWIRSGLKDFSISRASVEWGIPVPNDQKQTIYVW 300
YQ LE+ L QNP FVQP++RLNEVQSWI+SGL+DFSISRA V+WGIPVP+D KQTIYVW
Sbjct: 241 YQKPLEDILAQNPRFVQPSYRLNEVQSWIKSGLRDFSISRALVDWGIPVPDDDKQTIYVW 300
Query: 301 FDALLGYISALSENGQQQNLQRAVASGWPASLHLIGKDILRFHAVYWPAMLMSAGLKLPK 360
FDALLGYISAL+E+ +QQNL+ AV+ GWPASLHLIGKDILRFHAVYWPAMLMSAGL+LPK
Sbjct: 301 FDALLGYISALTEDNKQQNLETAVSFGWPASLHLIGKDILRFHAVYWPAMLMSAGLELPK 360
Query: 361 MVFGHGFLTKDGMKMGKSLGNTLEPNELVQKFGSDAVRYFFLQEVEFGSDGDYAEDRFIN 420
MVFGHGFLTKDGMKMGKSLGNTLEP ELVQKFG DAVRYFFL+EVEFG+DGDY+EDRFI
Sbjct: 361 MVFGHGFLTKDGMKMGKSLGNTLEPFELVQKFGPDAVRYFFLREVEFGNDGDYSEDRFIK 420
Query: 421 IVNAHLANTIGNLLNRTLGLLKKNCQSTLVVDSTAAAEGNSFKDKVEELVLKARNHYEGL 480
IVNAHLANTIGNLLNRTLGLLKKNC+STLVVDST AAEG KD VE+LV KAR +YE L
Sbjct: 421 IVNAHLANTIGNLLNRTLGLLKKNCESTLVVDSTVAAEGVPLKDTVEKLVEKARTNYENL 480
Query: 481 SLSSACEAVLEIGNAGNSYMDEQAPWSLFKQGGASSDAAAKDLVLILEAMRIIAVALSPV 540
SLSSACEAVLEIGNAGN+YMD++APW LFKQGG S++ AAKDLV+ILE MR+IAVALSPV
Sbjct: 481 SLSSACEAVLEIGNAGNTYMDQRAPWFLFKQGGVSAEEAAKDLVIILEVMRVIAVALSPV 540
Query: 541 TPCLGWRIYAQLGFSKDQFEAATWNETKWGGLKAGQVMAPPNPVFARIEQSTGSDEDE-- 600
PCL RIY+QLG+++DQF + TW++TKWGGLK GQVM +PVFARIE + +EDE
Sbjct: 541 APCLSLRIYSQLGYTEDQFNSITWSDTKWGGLKGGQVMEQASPVFARIELNPEKEEDEKK 600
Query: 601 --VPKKPVKSKKKIAQ---GVAEA 617
V KK K+K K+ + VAEA
Sbjct: 601 PKVGKKTGKAKVKVVEQTPTVAEA 616
BLAST of Moc04g34620 vs. ExPASy Swiss-Prot
Match:
Q55729 (Methionine--tRNA ligase OS=Synechocystis sp. (strain PCC 6803 / Kazusa) OX=1111708 GN=metG PE=3 SV=1)
HSP 1 Score: 560.8 bits (1444), Expect = 1.9e-158
Identity = 276/521 (52.98%), Postives = 357/521 (68.52%), Query Frame = 0
Query: 78 FVITTPLYYVNAAPHMGSAYTTIAADAIARFQRLLGKKVIFITGTDEHGEKIATAAAAQG 137
F +TTPLYYVN PH+GSAYTT+ AD +ARF+RL G V+ ITGTDEHG+KI A AQ
Sbjct: 9 FSVTTPLYYVNDVPHLGSAYTTVVADTLARFKRLQGYDVLMITGTDEHGQKIQRTAEAQE 68
Query: 138 SAPSEHCDAISEAYKTLWKDLDVAYDKFIRTTDPKHEAIVKEFYSKVLANGDIYRADYEG 197
P HCD +K LW+ L++ YD+F RTTDP+H AIVK+F+ +V GDIY A +G
Sbjct: 69 LDPQTHCDRTVVKFKELWRSLNILYDRFSRTTDPRHLAIVKDFFQRVWDKGDIYLAQQQG 128
Query: 198 LYCVNCEEYKDEKELLANNCCPIHLKPCAS-RKEDNYFFALSKYQGLLEETLTQNPGFVQ 257
YCV CEE+K++++LL +N CP+H A R E+NYFF LS+YQ LEE Q P F+Q
Sbjct: 129 WYCVACEEFKEKRDLLEDNHCPLHPNRKAEWRDEENYFFRLSRYQHPLEELYAQRPEFIQ 188
Query: 258 PAFRLNEVQSWIRSGLKDFSISRASVEWGIPVPNDQKQTIYVWFDALLGYISALSENGQQ 317
P+ R NEV +++ GL+DFSISR +++WG P+PND TIYVWFDALLGY++AL + ++
Sbjct: 189 PSSRRNEVLNFVAQGLQDFSISRVNLDWGFPLPNDPNHTIYVWFDALLGYVTALLDEDEE 248
Query: 318 QNLQRAVASGWPASLHLIGKDILRFHAVYWPAMLMSAGLKLPKMVFGHGFLTKDGMKMGK 377
NL A+ WP +LHLIGKDILRFHAVYWPAMLMSA L +P VFGHGFLTKDG KMGK
Sbjct: 249 PNLTNALVKWWPINLHLIGKDILRFHAVYWPAMLMSAELAIPAQVFGHGFLTKDGQKMGK 308
Query: 378 SLGNTLEPNELVQKFGSDAVRYFFLQEVEFGSDGDYAEDRFINIVNAHLANTIGNLLNRT 437
SLGNT++P +L+ ++G DA RY+FL+E+EFG DGD+ E RF+N++NA LAN +GNLLNRT
Sbjct: 309 SLGNTVDPLDLINRYGEDAFRYYFLKEIEFGKDGDFNEQRFVNVLNADLANDLGNLLNRT 368
Query: 438 LGLLKKNCQST-LVVDSTAAAEGNSFKDKVEELVLKARNHYEGLSLSSACEAVLEIGNAG 497
LG++KK CQ V +T A N K L + + YE LS + ACEA+ + AG
Sbjct: 369 LGMVKKYCQGQGPQVMATDLAPDNPLKALGSHLGEEVSSAYERLSFTDACEAIFTLVRAG 428
Query: 498 NSYMDEQAPWSLFKQGGASSDAAAKDLVLILEAMRIIAVALSPVTPCLGWRIYAQLGFSK 557
N Y+D+ APW LFKQG S L +LE++R+ A LSP+ P L +IY QLGF+
Sbjct: 429 NKYIDDMAPWKLFKQG--SQKEVEDVLYSVLESIRLSAYLLSPIVPRLSTKIYQQLGFTW 488
Query: 558 D-------QFEAATWN-ETKWGGLKAGQVMAPPNPVFARIE 589
D +A +N WG L A Q + P P+F ++E
Sbjct: 489 DFDQWRSPLEQAEEFNRHQSWGQLGANQNLPPAQPIFTKLE 527
BLAST of Moc04g34620 vs. ExPASy Swiss-Prot
Match:
Q8Z068 (Methionine--tRNA ligase OS=Nostoc sp. (strain PCC 7120 / SAG 25.82 / UTEX 2576) OX=103690 GN=metG PE=3 SV=1)
HSP 1 Score: 556.6 bits (1433), Expect = 3.5e-157
Identity = 277/523 (52.96%), Postives = 363/523 (69.41%), Query Frame = 0
Query: 74 ATDPFVITTPLYYVNAAPHMGSAYTTIAADAIARFQRLLGKKVIFITGTDEHGEKIATAA 133
A F +TTPLYYVN PH+GSAYTT+AADA+ARFQ+LLG+ V+ ITGTDEHG+KI +A
Sbjct: 7 AEKTFALTTPLYYVNDVPHIGSAYTTMAADAVARFQKLLGRDVLLITGTDEHGQKIQRSA 66
Query: 134 AAQGSAPSEHCDAISEAYKTLWKDLDVAYDKFIRTTDPKHEAIVKEFYSKVLANGDIYRA 193
+ G AP E CD I ++ +LW+ L++ YD+F RTT +H+AIV EF+++V GDIY+
Sbjct: 67 ESLGKAPQEFCDEIVPSFMSLWRLLNIQYDRFSRTTAVRHKAIVDEFFARVWEAGDIYQG 126
Query: 194 DYEGLYCVNCEEYKDEKELLANNCCPIHL-KPCASRKEDNYFFALSKYQGLLEETLTQNP 253
+G YCV+CEE+K+E+ELL N CPIH+ K R E NYFF LSKYQ LEE +P
Sbjct: 127 QQKGWYCVSCEEFKEERELLEGNRCPIHVNKEVEWRDEQNYFFRLSKYQTQLEEFYQSHP 186
Query: 254 GFVQPAFRLNEVQSWIRSGLKDFSISRASVEWGIPVPNDQKQTIYVWFDALLGYISALSE 313
F+QP R NEV +++ GL+DFSISR +++WG PVPND K T+YVWFDALL Y++AL +
Sbjct: 187 DFIQPESRRNEVLNFVSQGLQDFSISRVNLDWGFPVPNDPKHTLYVWFDALLAYVTALLD 246
Query: 314 NGQQQNLQRAVASGWPASLHLIGKDILRFHAVYWPAMLMSAGLKLPKMVFGHGFLTKDGM 373
+ L+ A+ WP +LHLIGKDILRFHAVYWPAML+SAGL LP VFGHGFLTKDG
Sbjct: 247 PEDEPTLENALGKWWPINLHLIGKDILRFHAVYWPAMLLSAGLPLPDRVFGHGFLTKDGQ 306
Query: 374 KMGKSLGNTLEPNELVQKFGSDAVRYFFLQEVEFGSDGDYAEDRFINIVNAHLANTIGNL 433
KMGKSLGNT++P LVQ++GSDAVRY+FL+E+EFG DGD+ E RFI+++NA LAN +GNL
Sbjct: 307 KMGKSLGNTVDPVGLVQQYGSDAVRYYFLKEIEFGKDGDFNEVRFIHVLNADLANDLGNL 366
Query: 434 LNRTLGLLKKNCQS-TLVVDSTAAAEGNSFKDKVEELVLKARNHYEGLSLSSACEAVLEI 493
LNRTL ++KK C + L + + N+ K +L K + YE L+ + AC VL +
Sbjct: 367 LNRTLNMVKKYCANYALSITNEDIPAENTLKALGVDLGAKVKQAYEVLAFNQACGVVLSL 426
Query: 494 GNAGNSYMDEQAPWSLFKQGGASSDAAAKDLVLILEAMRIIAVALSPVTPCLGWRIYAQL 553
A N ++D+QAPWSL+KQ L +LE++R+ A LSPV P + IY QL
Sbjct: 427 VQASNKFIDDQAPWSLYKQ--ERQQEVETVLYTVLESVRLAAYLLSPVIPNISSNIYQQL 486
Query: 554 GFS---KDQFEAATWN-ETKWGGLKAGQVMAPPNPVFARIEQS 591
GF +Q + + +WG L Q + P P+F RIEQ+
Sbjct: 487 GFGINFNEQTDITPFAIHAQWGLLSNKQQLGQPQPIFKRIEQT 527
BLAST of Moc04g34620 vs. ExPASy Swiss-Prot
Match:
P59081 (Methionine--tRNA ligase OS=Thermosynechococcus elongatus (strain BP-1) OX=197221 GN=metG PE=3 SV=1)
HSP 1 Score: 531.2 bits (1367), Expect = 1.6e-149
Identity = 263/518 (50.77%), Postives = 346/518 (66.80%), Query Frame = 0
Query: 78 FVITTPLYYVNAAPHMGSAYTTIAADAIARFQRLLGKKVIFITGTDEHGEKIATAAAAQG 137
F +TTPLYYVNA PH+GSAYTTIAAD +ARF RL G +V FITGTDEHG+KI A +G
Sbjct: 5 FSLTTPLYYVNALPHIGSAYTTIAADVLARFYRLQGYQVRFITGTDEHGQKIERTAQQRG 64
Query: 138 SAPSEHCDAISEAYKTLWKDLDVAYDKFIRTTDPKHEAIVKEFYSKVLANGDIYRADYEG 197
+P HCD I+ ++ LW+ L++ YD+F RTT P+H AIV EF+ +V NGDIY +G
Sbjct: 65 LSPQAHCDEIAAGFQALWQQLNIHYDRFSRTTSPRHHAIVNEFFQRVWDNGDIYLGQQQG 124
Query: 198 LYCVNCEEYKDEKELLANNCCPIHLKPCAS-RKEDNYFFALSKYQGLLEETLTQNPGFVQ 257
YCV CEE+K+E+ELL CPIH+ R E NYFF LSKYQ L + ++P FVQ
Sbjct: 125 WYCVECEEFKEERELLEGRRCPIHVNRTVEWRDERNYFFRLSKYQQALLDHYAEHPDFVQ 184
Query: 258 PAFRLNEVQSWIRSGLKDFSISRASVEWGIPVPNDQKQTIYVWFDALLGYISALSENGQQ 317
P R NEV S+I GL+DFSISR ++ WG PVP D +QT+YVWFDALLGY++AL E +
Sbjct: 185 PPSRRNEVLSFIERGLQDFSISRVNLAWGFPVPTDPEQTLYVWFDALLGYVTALLEPEDE 244
Query: 318 QNLQRAVASGWPASLHLIGKDILRFHAVYWPAMLMSAGLKLPKMVFGHGFLTKDGMKMGK 377
L A+ + WP +LH+IGKDILRFH + WPAMLMSAGL LP+ +F HGFLTKDG KMGK
Sbjct: 245 PTLANALKTWWPINLHIIGKDILRFHGISWPAMLMSAGLPLPEQIFVHGFLTKDGQKMGK 304
Query: 378 SLGNTLEPNELVQKFGSDAVRYFFLQEVEFGSDGDYAEDRFINIVNAHLANTIGNLLNRT 437
SLGNTL+P LV ++G+DAVRY+F++EVEFG DGD++E RF+ I+NA LAN +GNLLNRT
Sbjct: 305 SLGNTLDPFALVAQYGADAVRYYFMKEVEFGRDGDFSETRFVTILNADLANDLGNLLNRT 364
Query: 438 LGLLKKNCQSTLV-VDSTAAAEGNSFKDKVEELVLKARNHYEGLSLSSACEAVLEIGNAG 497
L + K + V A + + E L Y L+ C+ L + AG
Sbjct: 365 LKMAWKYTDGKVPNVQGAAIPREHPLRQLAEHLSQTYGQGYRQLAFHEVCQQALTLARAG 424
Query: 498 NSYMDEQAPWSLFKQGGASSDAAAKDLVLILEAMRIIAVALSPVTPCLGWRIYAQLGFSK 557
N ++DE+APW ++ G + A A+ L +LE++R++A L+P+ P L IY QLG+S
Sbjct: 425 NKFLDEEAPWKRYRAG--ETAAVAEILYCVLESVRLVAYVLAPIIPQLSEAIYGQLGYS- 484
Query: 558 DQFEAATW------NETKWGGLKAGQVMAPPNPVFARI 588
+F + + +WG L A Q +A P P+F ++
Sbjct: 485 IRFNGSIAPDLLGDRQAQWGVLPASQPLANPEPIFQKL 519
BLAST of Moc04g34620 vs. ExPASy Swiss-Prot
Match:
O67298 (Methionine--tRNA ligase OS=Aquifex aeolicus (strain VF5) OX=224324 GN=metG PE=1 SV=1)
HSP 1 Score: 431.8 bits (1109), Expect = 1.3e-119
Identity = 217/466 (46.57%), Postives = 291/466 (62.45%), Query Frame = 0
Query: 78 FVITTPLYYVNAAPHMGSAYTTIAADAIARFQRLLGKKVIFITGTDEHGEKIATAAAAQG 137
F +TTP+YYVN PH+G AYTTIAAD IAR+ RL V F+TGTDEHG KI A G
Sbjct: 7 FYVTTPIYYVNDVPHLGHAYTTIAADTIARYYRLRDYDVFFLTGTDEHGLKIQKKAEELG 66
Query: 138 SAPSEHCDAISEAYKTLWKDLDVAYDKFIRTTDPKHEAIVKEFYSKVLANGDIYRADYEG 197
+P E D +E +K LW+ L + Y KFIRTTDP H V++ + + GDIY +YEG
Sbjct: 67 ISPKELVDRNAERFKKLWEFLKIEYTKFIRTTDPYHVKFVQKVFEECYKRGDIYLGEYEG 126
Query: 198 LYCVNCEEYKDEKELLANNCCPIHLKPCASRKEDNYFFALSKYQGLLEETLTQNPGFVQP 257
YCV CEE+K E EL ++ CPIH K C KE +YFF LSKYQ L E +NP F+QP
Sbjct: 127 WYCVGCEEFKSEAELAEDHTCPIHQKKCEYIKEPSYFFRLSKYQDKLLELYEKNPEFIQP 186
Query: 258 AFRLNEVQSWIRSGLKDFSIS--RASVEWGIPVPNDQKQTIYVWFDALLGYISALSENGQ 317
+R NE+ S+++ GLKD S++ R+ V+WGIPVP D + TIYVWFDAL YISAL +
Sbjct: 187 DYRRNEIISFVKQGLKDLSVTRPRSRVKWGIPVPFDPEHTIYVWFDALFNYISALEDK-- 246
Query: 318 QQNLQRAVASGWPASLHLIGKDILRFHAVYWPAMLMSAGLKLPKMVFGHGFLTKDGMKMG 377
V WPA LHL+GKDILRFH VYWPA LMS G +LPK VF HG+ T +G KM
Sbjct: 247 -------VEIYWPADLHLVGKDILRFHTVYWPAFLMSLGYELPKKVFAHGWWTVEGKKMS 306
Query: 378 KSLGNTLEPNELVQKFGSDAVRYFFLQEVEFGSDGDYAEDRFINIVNAHLANTIGNLLNR 437
K+LGN ++P E+VQ++G D VRYF L+EV FG DGD+++ +N +N LAN IGNL +R
Sbjct: 307 KTLGNVVDPYEVVQEYGLDEVRYFLLREVPFGQDGDFSKKAILNRINGELANEIGNLYSR 366
Query: 438 TLGLLKKNCQSTLVVDSTAAAEGNSFKDKVEELVLKARNHYEGLSLSSACEAVLEIGNAG 497
+ + K + + A + +E + N+ E ++ A E +L+ +
Sbjct: 367 VVNMAHK-----FLGGEVSGARDEEYAKIAQESIKNYENYMEKVNFYKAIEEILKFTSYL 426
Query: 498 NSYMDEQAPWSLFKQGGASSDAAAKDLVLILEAMRIIAVALSPVTP 542
N Y+DE+ PW+L K+ + K L +++ + ++ L P+TP
Sbjct: 427 NKYVDEKQPWALNKE--RKKEELQKVLYALVDGLFVLTHLLYPITP 456
BLAST of Moc04g34620 vs. ExPASy TrEMBL
Match:
A0A6J1BXG8 (Methionine--tRNA ligase OS=Momordica charantia OX=3673 GN=LOC111006379 PE=3 SV=1)
HSP 1 Score: 1232.2 bits (3187), Expect = 0.0e+00
Identity = 616/616 (100.00%), Postives = 616/616 (100.00%), Query Frame = 0
Query: 1 MAARINSSIQSSLWLLNPLQSSLNLNLNLNLNLRTRLNFYRNLLPSPSYSASSSKRAIFC 60
MAARINSSIQSSLWLLNPLQSSLNLNLNLNLNLRTRLNFYRNLLPSPSYSASSSKRAIFC
Sbjct: 1 MAARINSSIQSSLWLLNPLQSSLNLNLNLNLNLRTRLNFYRNLLPSPSYSASSSKRAIFC 60
Query: 61 TYASNCNSHESGAATDPFVITTPLYYVNAAPHMGSAYTTIAADAIARFQRLLGKKVIFIT 120
TYASNCNSHESGAATDPFVITTPLYYVNAAPHMGSAYTTIAADAIARFQRLLGKKVIFIT
Sbjct: 61 TYASNCNSHESGAATDPFVITTPLYYVNAAPHMGSAYTTIAADAIARFQRLLGKKVIFIT 120
Query: 121 GTDEHGEKIATAAAAQGSAPSEHCDAISEAYKTLWKDLDVAYDKFIRTTDPKHEAIVKEF 180
GTDEHGEKIATAAAAQGSAPSEHCDAISEAYKTLWKDLDVAYDKFIRTTDPKHEAIVKEF
Sbjct: 121 GTDEHGEKIATAAAAQGSAPSEHCDAISEAYKTLWKDLDVAYDKFIRTTDPKHEAIVKEF 180
Query: 181 YSKVLANGDIYRADYEGLYCVNCEEYKDEKELLANNCCPIHLKPCASRKEDNYFFALSKY 240
YSKVLANGDIYRADYEGLYCVNCEEYKDEKELLANNCCPIHLKPCASRKEDNYFFALSKY
Sbjct: 181 YSKVLANGDIYRADYEGLYCVNCEEYKDEKELLANNCCPIHLKPCASRKEDNYFFALSKY 240
Query: 241 QGLLEETLTQNPGFVQPAFRLNEVQSWIRSGLKDFSISRASVEWGIPVPNDQKQTIYVWF 300
QGLLEETLTQNPGFVQPAFRLNEVQSWIRSGLKDFSISRASVEWGIPVPNDQKQTIYVWF
Sbjct: 241 QGLLEETLTQNPGFVQPAFRLNEVQSWIRSGLKDFSISRASVEWGIPVPNDQKQTIYVWF 300
Query: 301 DALLGYISALSENGQQQNLQRAVASGWPASLHLIGKDILRFHAVYWPAMLMSAGLKLPKM 360
DALLGYISALSENGQQQNLQRAVASGWPASLHLIGKDILRFHAVYWPAMLMSAGLKLPKM
Sbjct: 301 DALLGYISALSENGQQQNLQRAVASGWPASLHLIGKDILRFHAVYWPAMLMSAGLKLPKM 360
Query: 361 VFGHGFLTKDGMKMGKSLGNTLEPNELVQKFGSDAVRYFFLQEVEFGSDGDYAEDRFINI 420
VFGHGFLTKDGMKMGKSLGNTLEPNELVQKFGSDAVRYFFLQEVEFGSDGDYAEDRFINI
Sbjct: 361 VFGHGFLTKDGMKMGKSLGNTLEPNELVQKFGSDAVRYFFLQEVEFGSDGDYAEDRFINI 420
Query: 421 VNAHLANTIGNLLNRTLGLLKKNCQSTLVVDSTAAAEGNSFKDKVEELVLKARNHYEGLS 480
VNAHLANTIGNLLNRTLGLLKKNCQSTLVVDSTAAAEGNSFKDKVEELVLKARNHYEGLS
Sbjct: 421 VNAHLANTIGNLLNRTLGLLKKNCQSTLVVDSTAAAEGNSFKDKVEELVLKARNHYEGLS 480
Query: 481 LSSACEAVLEIGNAGNSYMDEQAPWSLFKQGGASSDAAAKDLVLILEAMRIIAVALSPVT 540
LSSACEAVLEIGNAGNSYMDEQAPWSLFKQGGASSDAAAKDLVLILEAMRIIAVALSPVT
Sbjct: 481 LSSACEAVLEIGNAGNSYMDEQAPWSLFKQGGASSDAAAKDLVLILEAMRIIAVALSPVT 540
Query: 541 PCLGWRIYAQLGFSKDQFEAATWNETKWGGLKAGQVMAPPNPVFARIEQSTGSDEDEVPK 600
PCLGWRIYAQLGFSKDQFEAATWNETKWGGLKAGQVMAPPNPVFARIEQSTGSDEDEVPK
Sbjct: 541 PCLGWRIYAQLGFSKDQFEAATWNETKWGGLKAGQVMAPPNPVFARIEQSTGSDEDEVPK 600
Query: 601 KPVKSKKKIAQGVAEA 617
KPVKSKKKIAQGVAEA
Sbjct: 601 KPVKSKKKIAQGVAEA 616
BLAST of Moc04g34620 vs. ExPASy TrEMBL
Match:
A0A0A0KQV7 (Methionine--tRNA ligase OS=Cucumis sativus OX=3659 GN=Csa_5G512910 PE=3 SV=1)
HSP 1 Score: 1110.9 bits (2872), Expect = 0.0e+00
Identity = 557/616 (90.42%), Postives = 584/616 (94.81%), Query Frame = 0
Query: 1 MAARINSSIQSSLWLLNPLQSSLNLNLNLNLNLRTRLNFYRNLLPSPSYSASSSKRAIFC 60
MAARIN+SIQ+SLWLLNP SSLNL + RTRL+F +NLL PS+ SSSKRAIFC
Sbjct: 1 MAARINTSIQTSLWLLNPFHSSLNLK---PTHFRTRLHFCQNLLSFPSH--SSSKRAIFC 60
Query: 61 TYASNCNSHESGAATDPFVITTPLYYVNAAPHMGSAYTTIAADAIARFQRLLGKKVIFIT 120
T SN NSH+SGAATDPFVITTPLYYVNAAPHMGSAYTTIAADA+ARFQRLLGKKVIFIT
Sbjct: 61 TSTSNFNSHDSGAATDPFVITTPLYYVNAAPHMGSAYTTIAADAVARFQRLLGKKVIFIT 120
Query: 121 GTDEHGEKIATAAAAQGSAPSEHCDAISEAYKTLWKDLDVAYDKFIRTTDPKHEAIVKEF 180
GTDEHGEKIATAAA QGS PSEHCD ISEAYKTLWKDLDVAYDKFIRTTDPKHEAIVKEF
Sbjct: 121 GTDEHGEKIATAAATQGSTPSEHCDIISEAYKTLWKDLDVAYDKFIRTTDPKHEAIVKEF 180
Query: 181 YSKVLANGDIYRADYEGLYCVNCEEYKDEKELLANNCCPIHLKPCASRKEDNYFFALSKY 240
YSKVLANGDIYRADYEGLYCVNCEEYKDEKELLA+NCCPIHLKPCASRKEDNYFFALSKY
Sbjct: 181 YSKVLANGDIYRADYEGLYCVNCEEYKDEKELLADNCCPIHLKPCASRKEDNYFFALSKY 240
Query: 241 QGLLEETLTQNPGFVQPAFRLNEVQSWIRSGLKDFSISRASVEWGIPVPNDQKQTIYVWF 300
Q LLEETLTQNPGFVQPAFRLNEVQSWIRSGLKDFSISRASVEWGIPVPND+KQTIYVWF
Sbjct: 241 QRLLEETLTQNPGFVQPAFRLNEVQSWIRSGLKDFSISRASVEWGIPVPNDEKQTIYVWF 300
Query: 301 DALLGYISALSENGQQQNLQRAVASGWPASLHLIGKDILRFHAVYWPAMLMSAGLKLPKM 360
DALLGYISALSENG+Q NLQ VASGWPASLHLIGKDILRFHAVYWPAMLMSAGLKLPKM
Sbjct: 301 DALLGYISALSENGEQPNLQEVVASGWPASLHLIGKDILRFHAVYWPAMLMSAGLKLPKM 360
Query: 361 VFGHGFLTKDGMKMGKSLGNTLEPNELVQKFGSDAVRYFFLQEVEFGSDGDYAEDRFINI 420
VFGHGFLTKDGMKMGKSLGNTLEPNELV+KFGSDAVRYFFL+EVEFGSDGDYAEDRFINI
Sbjct: 361 VFGHGFLTKDGMKMGKSLGNTLEPNELVEKFGSDAVRYFFLREVEFGSDGDYAEDRFINI 420
Query: 421 VNAHLANTIGNLLNRTLGLLKKNCQSTLVVDSTAAAEGNSFKDKVEELVLKARNHYEGLS 480
VNAHLANTIGNLLNRTLGLLKKNCQSTLVVDSTAAA+GN+FKDKVEELV+KARN+YE L+
Sbjct: 421 VNAHLANTIGNLLNRTLGLLKKNCQSTLVVDSTAAADGNAFKDKVEELVVKARNNYEELA 480
Query: 481 LSSACEAVLEIGNAGNSYMDEQAPWSLFKQGGASSDAAAKDLVLILEAMRIIAVALSPVT 540
LSSACEAVLEIGNAGNSYMDE+APWSLFKQGGASS+AAAKDLVLILEAMRIIA+ALSPVT
Sbjct: 481 LSSACEAVLEIGNAGNSYMDERAPWSLFKQGGASSNAAAKDLVLILEAMRIIAIALSPVT 540
Query: 541 PCLGWRIYAQLGFSKDQFEAATWNETKWGGLKAGQVMAPPNPVFARIEQSTGSDEDEVPK 600
P L WRIYAQLG+SKDQF+AATWNETKWGGLKAGQVMAPPNPVFARIEQ+ + E+E PK
Sbjct: 541 PRLSWRIYAQLGYSKDQFDAATWNETKWGGLKAGQVMAPPNPVFARIEQAIVNGEEEAPK 600
Query: 601 KPVKSKKKIAQGVAEA 617
KPVKSKKKIAQ VAE+
Sbjct: 601 KPVKSKKKIAQAVAES 611
BLAST of Moc04g34620 vs. ExPASy TrEMBL
Match:
A0A5D3C1K0 (Methionine--tRNA ligase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold98G003330 PE=3 SV=1)
HSP 1 Score: 1108.6 bits (2866), Expect = 0.0e+00
Identity = 557/616 (90.42%), Postives = 584/616 (94.81%), Query Frame = 0
Query: 1 MAARINSSIQSSLWLLNPLQSSLNLNLNLNLNLRTRLNFYRNLLPSPSYSASSSKRAIFC 60
MAARIN+SIQ+SL LLNP +SLNL + RTRL+FY+NLL SY SSSKRAIFC
Sbjct: 1 MAARINTSIQTSLSLLNPFHNSLNLK---PTHFRTRLHFYQNLLSFASY--SSSKRAIFC 60
Query: 61 TYASNCNSHESGAATDPFVITTPLYYVNAAPHMGSAYTTIAADAIARFQRLLGKKVIFIT 120
T SN NSH++GAATDPFVITTPLYYVNAAPHMGSAYTTIAADAIARFQRLLGKKVI IT
Sbjct: 61 TTTSNFNSHDTGAATDPFVITTPLYYVNAAPHMGSAYTTIAADAIARFQRLLGKKVILIT 120
Query: 121 GTDEHGEKIATAAAAQGSAPSEHCDAISEAYKTLWKDLDVAYDKFIRTTDPKHEAIVKEF 180
GTDEHGEKIATAAA QGS PSEHCD ISEAYKTLWKDLDVAYDKFIRTTDPKHEAIVKEF
Sbjct: 121 GTDEHGEKIATAAATQGSTPSEHCDIISEAYKTLWKDLDVAYDKFIRTTDPKHEAIVKEF 180
Query: 181 YSKVLANGDIYRADYEGLYCVNCEEYKDEKELLANNCCPIHLKPCASRKEDNYFFALSKY 240
YSKVLANGDIYRADYEGLYCVNCEEYKDEKELLA+NCCPIHLKPCASRKEDNYFFALSKY
Sbjct: 181 YSKVLANGDIYRADYEGLYCVNCEEYKDEKELLADNCCPIHLKPCASRKEDNYFFALSKY 240
Query: 241 QGLLEETLTQNPGFVQPAFRLNEVQSWIRSGLKDFSISRASVEWGIPVPNDQKQTIYVWF 300
Q LLEETLTQNPGFVQPAFRLNEVQSWIRSGLKDFSISRASVEWGIPVPND+KQTIYVWF
Sbjct: 241 QRLLEETLTQNPGFVQPAFRLNEVQSWIRSGLKDFSISRASVEWGIPVPNDKKQTIYVWF 300
Query: 301 DALLGYISALSENGQQQNLQRAVASGWPASLHLIGKDILRFHAVYWPAMLMSAGLKLPKM 360
DALLGYISALSENG+Q NL + VASGWPASLHLIGKDILRFHAVYWPAMLMSAGLKLPKM
Sbjct: 301 DALLGYISALSENGEQPNLHKVVASGWPASLHLIGKDILRFHAVYWPAMLMSAGLKLPKM 360
Query: 361 VFGHGFLTKDGMKMGKSLGNTLEPNELVQKFGSDAVRYFFLQEVEFGSDGDYAEDRFINI 420
VFGHGFLTKDGMKMGKSLGNTLEPNELV+KFGSDAVRYFFL+EVEFGSDGDYAEDRFINI
Sbjct: 361 VFGHGFLTKDGMKMGKSLGNTLEPNELVEKFGSDAVRYFFLREVEFGSDGDYAEDRFINI 420
Query: 421 VNAHLANTIGNLLNRTLGLLKKNCQSTLVVDSTAAAEGNSFKDKVEELVLKARNHYEGLS 480
VNAHLANTIGNLLNRTLGLLKKNCQSTL+VDSTAAA+GN+FKDKVEELV+KARNHYE L+
Sbjct: 421 VNAHLANTIGNLLNRTLGLLKKNCQSTLLVDSTAAADGNAFKDKVEELVVKARNHYEELA 480
Query: 481 LSSACEAVLEIGNAGNSYMDEQAPWSLFKQGGASSDAAAKDLVLILEAMRIIAVALSPVT 540
LSSACEAVLEIGNAGNSYMDE+APWSLFKQGGASS+AAAKDLVLILEAMRIIA+ALSPVT
Sbjct: 481 LSSACEAVLEIGNAGNSYMDERAPWSLFKQGGASSNAAAKDLVLILEAMRIIAIALSPVT 540
Query: 541 PCLGWRIYAQLGFSKDQFEAATWNETKWGGLKAGQVMAPPNPVFARIEQSTGSDEDEVPK 600
P L WRIYAQLG+SKDQF+AATWNETKWGGLKAGQVMAPPNPVFARIEQ+ + E+EVPK
Sbjct: 541 PRLSWRIYAQLGYSKDQFDAATWNETKWGGLKAGQVMAPPNPVFARIEQAIVNGEEEVPK 600
Query: 601 KPVKSKKKIAQGVAEA 617
KPVKSKKKIAQGVAEA
Sbjct: 601 KPVKSKKKIAQGVAEA 611
BLAST of Moc04g34620 vs. ExPASy TrEMBL
Match:
A0A6J1H8X3 (Methionine--tRNA ligase OS=Cucurbita moschata OX=3662 GN=LOC111461165 PE=3 SV=1)
HSP 1 Score: 1108.2 bits (2865), Expect = 0.0e+00
Identity = 558/617 (90.44%), Postives = 581/617 (94.17%), Query Frame = 0
Query: 1 MAARINSSIQSSLWLLNPLQSSLNLNLNLNLNLRTRLNFYRNLLPSP-SYSASSSKRAIF 60
MAARIN+SIQ+SLWLLNPLQSS NL +LRTR NFY+NLL +P S S+SSSKRAIF
Sbjct: 1 MAARINTSIQNSLWLLNPLQSSRNLKAT---HLRTRFNFYQNLLSAPSSCSSSSSKRAIF 60
Query: 61 CTYASNCNSHESGAATDPFVITTPLYYVNAAPHMGSAYTTIAADAIARFQRLLGKKVIFI 120
C+ ASN NSH+ GAATDPFVITTPLYYVNAAPHMGSAYTTIAADAIARFQRLLGKKVIFI
Sbjct: 61 CSSASNFNSHDGGAATDPFVITTPLYYVNAAPHMGSAYTTIAADAIARFQRLLGKKVIFI 120
Query: 121 TGTDEHGEKIATAAAAQGSAPSEHCDAISEAYKTLWKDLDVAYDKFIRTTDPKHEAIVKE 180
TGTDEHGEKIATAAAAQGS PSEHCD ISEAYKTLWKDLD+AYDKFIRTTDPKHE IVKE
Sbjct: 121 TGTDEHGEKIATAAAAQGSGPSEHCDNISEAYKTLWKDLDIAYDKFIRTTDPKHETIVKE 180
Query: 181 FYSKVLANGDIYRADYEGLYCVNCEEYKDEKELLANNCCPIHLKPCASRKEDNYFFALSK 240
FYS VLANGDIYRADYEGLYCVNCEEYKDEKELLA+NCCPIHLKPCASRKEDNYFFALSK
Sbjct: 181 FYSNVLANGDIYRADYEGLYCVNCEEYKDEKELLADNCCPIHLKPCASRKEDNYFFALSK 240
Query: 241 YQGLLEETLTQNPGFVQPAFRLNEVQSWIRSGLKDFSISRASVEWGIPVPNDQKQTIYVW 300
YQ LLEETLTQNPGFVQPAFRLNEVQSWI+SGLKDFSISRASVEWGIPVPNDQKQTIYVW
Sbjct: 241 YQRLLEETLTQNPGFVQPAFRLNEVQSWIKSGLKDFSISRASVEWGIPVPNDQKQTIYVW 300
Query: 301 FDALLGYISALSENGQQQNLQRAVASGWPASLHLIGKDILRFHAVYWPAMLMSAGLKLPK 360
FDALLGYISALSEN +Q NLQ AVASGWPASLHLIGKDILRFHAVYWPAMLMSAGLKLPK
Sbjct: 301 FDALLGYISALSENDEQPNLQTAVASGWPASLHLIGKDILRFHAVYWPAMLMSAGLKLPK 360
Query: 361 MVFGHGFLTKDGMKMGKSLGNTLEPNELVQKFGSDAVRYFFLQEVEFGSDGDYAEDRFIN 420
MVFGHGFLTKDGMKMGKSLGNTLEPNELVQKFGSD VRYFFL+EVEFGSDGDYAEDRFIN
Sbjct: 361 MVFGHGFLTKDGMKMGKSLGNTLEPNELVQKFGSDPVRYFFLREVEFGSDGDYAEDRFIN 420
Query: 421 IVNAHLANTIGNLLNRTLGLLKKNCQSTLVVDSTAAAEGNSFKDKVEELVLKARNHYEGL 480
IVNAHLANTIGNLLNRTLGLLKKNCQSTLVVDS+AAA GN+FK+KVEELV KARNHYE L
Sbjct: 421 IVNAHLANTIGNLLNRTLGLLKKNCQSTLVVDSSAAAVGNAFKEKVEELVAKARNHYEEL 480
Query: 481 SLSSACEAVLEIGNAGNSYMDEQAPWSLFKQGGASSDAAAKDLVLILEAMRIIAVALSPV 540
SLSSACEAVLEIGNAGNSYMDE+APWSLFKQGGASSDAAAKDLVLILEAMRIIA+ALSPV
Sbjct: 481 SLSSACEAVLEIGNAGNSYMDERAPWSLFKQGGASSDAAAKDLVLILEAMRIIAIALSPV 540
Query: 541 TPCLGWRIYAQLGFSKDQFEAATWNETKWGGLKAGQVMAPPNPVFARIEQSTGSDEDEVP 600
TP L WRIY QLG+ +DQF AATWNETKWGGLKAGQVMAPPNPVFARIEQ+ + E+E P
Sbjct: 541 TPRLSWRIYEQLGYPEDQFAAATWNETKWGGLKAGQVMAPPNPVFARIEQAIVNGEEEAP 600
Query: 601 KKPVKSKKKIAQGVAEA 617
KKPVKSKKKIAQ VAEA
Sbjct: 601 KKPVKSKKKIAQKVAEA 614
BLAST of Moc04g34620 vs. ExPASy TrEMBL
Match:
A0A1S3CJS7 (Methionine--tRNA ligase OS=Cucumis melo OX=3656 GN=LOC103501636 PE=3 SV=1)
HSP 1 Score: 1107.8 bits (2864), Expect = 0.0e+00
Identity = 557/616 (90.42%), Postives = 583/616 (94.64%), Query Frame = 0
Query: 1 MAARINSSIQSSLWLLNPLQSSLNLNLNLNLNLRTRLNFYRNLLPSPSYSASSSKRAIFC 60
MAARIN+SIQ+SL LLNP +SLNL + RTRL+FY+NLL SY SSSKRAIFC
Sbjct: 1 MAARINTSIQTSLSLLNPFHNSLNLK---PTHFRTRLHFYQNLLSFASY--SSSKRAIFC 60
Query: 61 TYASNCNSHESGAATDPFVITTPLYYVNAAPHMGSAYTTIAADAIARFQRLLGKKVIFIT 120
T SN NSH++GAATDPFVITTPLYYVNAAPHMGSAYTTIAADAIARFQRLLGKKVI IT
Sbjct: 61 TTTSNFNSHDTGAATDPFVITTPLYYVNAAPHMGSAYTTIAADAIARFQRLLGKKVILIT 120
Query: 121 GTDEHGEKIATAAAAQGSAPSEHCDAISEAYKTLWKDLDVAYDKFIRTTDPKHEAIVKEF 180
GTDEHGEKIATAAA QGS PSEHCD ISEAYKTLWKDLDVAYDKFIRTTDPKHEAIVKEF
Sbjct: 121 GTDEHGEKIATAAATQGSTPSEHCDIISEAYKTLWKDLDVAYDKFIRTTDPKHEAIVKEF 180
Query: 181 YSKVLANGDIYRADYEGLYCVNCEEYKDEKELLANNCCPIHLKPCASRKEDNYFFALSKY 240
YSKVLANGDIYRADYEGLYCVNCEEYKDEKELLA+NCCPIHLKPCASRKEDNYFFALSKY
Sbjct: 181 YSKVLANGDIYRADYEGLYCVNCEEYKDEKELLADNCCPIHLKPCASRKEDNYFFALSKY 240
Query: 241 QGLLEETLTQNPGFVQPAFRLNEVQSWIRSGLKDFSISRASVEWGIPVPNDQKQTIYVWF 300
Q LLEETLTQNPGFVQPAFRLNEVQSWIRSGLKDFSISRASVEWGIPVPND KQTIYVWF
Sbjct: 241 QRLLEETLTQNPGFVQPAFRLNEVQSWIRSGLKDFSISRASVEWGIPVPNDXKQTIYVWF 300
Query: 301 DALLGYISALSENGQQQNLQRAVASGWPASLHLIGKDILRFHAVYWPAMLMSAGLKLPKM 360
DALLGYISALSENG+Q NL + VASGWPASLHLIGKDILRFHAVYWPAMLMSAGLKLPKM
Sbjct: 301 DALLGYISALSENGEQPNLHKVVASGWPASLHLIGKDILRFHAVYWPAMLMSAGLKLPKM 360
Query: 361 VFGHGFLTKDGMKMGKSLGNTLEPNELVQKFGSDAVRYFFLQEVEFGSDGDYAEDRFINI 420
VFGHGFLTKDGMKMGKSLGNTLEPNELV+KFGSDAVRYFFL+EVEFGSDGDYAEDRFINI
Sbjct: 361 VFGHGFLTKDGMKMGKSLGNTLEPNELVEKFGSDAVRYFFLREVEFGSDGDYAEDRFINI 420
Query: 421 VNAHLANTIGNLLNRTLGLLKKNCQSTLVVDSTAAAEGNSFKDKVEELVLKARNHYEGLS 480
VNAHLANTIGNLLNRTLGLLKKNCQSTL+VDSTAAA+GN+FKDKVEELV+KARNHYE L+
Sbjct: 421 VNAHLANTIGNLLNRTLGLLKKNCQSTLLVDSTAAADGNAFKDKVEELVVKARNHYEELA 480
Query: 481 LSSACEAVLEIGNAGNSYMDEQAPWSLFKQGGASSDAAAKDLVLILEAMRIIAVALSPVT 540
LSSACEAVLEIGNAGNSYMDE+APWSLFKQGGASS+AAAKDLVLILEAMRIIA+ALSPVT
Sbjct: 481 LSSACEAVLEIGNAGNSYMDERAPWSLFKQGGASSNAAAKDLVLILEAMRIIAIALSPVT 540
Query: 541 PCLGWRIYAQLGFSKDQFEAATWNETKWGGLKAGQVMAPPNPVFARIEQSTGSDEDEVPK 600
P L WRIYAQLG+SKDQF+AATWNETKWGGLKAGQVMAPPNPVFARIEQ+ + E+EVPK
Sbjct: 541 PRLSWRIYAQLGYSKDQFDAATWNETKWGGLKAGQVMAPPNPVFARIEQAIVNGEEEVPK 600
Query: 601 KPVKSKKKIAQGVAEA 617
KPVKSKKKIAQGVAEA
Sbjct: 601 KPVKSKKKIAQGVAEA 611
BLAST of Moc04g34620 vs. TAIR 10
Match:
AT3G55400.1 (methionyl-tRNA synthetase / methionine--tRNA ligase / MetRS (cpMetRS) )
HSP 1 Score: 911.4 bits (2354), Expect = 4.0e-265
Identity = 461/624 (73.88%), Postives = 524/624 (83.97%), Query Frame = 0
Query: 1 MAARINSSIQSSLWLLNPLQSSLNLNLNLNLNLRTRLNFYRNLLPSPSYSA-SSSKRAIF 60
MAARIN+S+ ++L L P + LN R N +R P YS SS KRA++
Sbjct: 1 MAARINTSLHNALSFLKPFNTPLNTK-----PFSFRRNSFRFSKKLPYYSQFSSGKRALY 60
Query: 61 CTYASNCNSHESGAATDPFVITTPLYYVNAAPHMGSAYTTIAADAIARFQRLLGKKVIFI 120
CT +S ++ + G + FV+TTPLYYVNA PHMGSAYTTIAAD+IARFQRLLGKKVIFI
Sbjct: 61 CTSSSQESTVDEG---ETFVLTTPLYYVNAPPHMGSAYTTIAADSIARFQRLLGKKVIFI 120
Query: 121 TGTDEHGEKIATAAAAQGSAPSEHCDAISEAYKTLWKDLDVAYDKFIRTTDPKHEAIVKE 180
TGTDEHGEKIAT+AAA G P EHCD IS++Y+TLWKDLD+AYDKFIRTTDPKHEAIVKE
Sbjct: 121 TGTDEHGEKIATSAAANGRNPPEHCDLISQSYRTLWKDLDIAYDKFIRTTDPKHEAIVKE 180
Query: 181 FYSKVLANGDIYRADYEGLYCVNCEEYKDEKELLANNCCPIHLKPCASRKEDNYFFALSK 240
FY++V ANGDIYRADYEGLYCVNCEEYKDEKELL NNCCP+H PC +RKEDNYFFALSK
Sbjct: 181 FYARVFANGDIYRADYEGLYCVNCEEYKDEKELLENNCCPVHQMPCVARKEDNYFFALSK 240
Query: 241 YQGLLEETLTQNPGFVQPAFRLNEVQSWIRSGLKDFSISRASVEWGIPVPNDQKQTIYVW 300
YQ LE+ L QNP FVQP++RLNEVQSWI+SGL+DFSISRA V+WGIPVP+D KQTIYVW
Sbjct: 241 YQKPLEDILAQNPRFVQPSYRLNEVQSWIKSGLRDFSISRALVDWGIPVPDDDKQTIYVW 300
Query: 301 FDALLGYISALSENGQQQNLQRAVASGWPASLHLIGKDILRFHAVYWPAMLMSAGLKLPK 360
FDALLGYISAL+E+ +QQNL+ AV+ GWPASLHLIGKDILRFHAVYWPAMLMSAGL+LPK
Sbjct: 301 FDALLGYISALTEDNKQQNLETAVSFGWPASLHLIGKDILRFHAVYWPAMLMSAGLELPK 360
Query: 361 MVFGHGFLTKDGMKMGKSLGNTLEPNELVQKFGSDAVRYFFLQEVEFGSDGDYAEDRFIN 420
MVFGHGFLTKDGMKMGKSLGNTLEP ELVQKFG DAVRYFFL+EVEFG+DGDY+EDRFI
Sbjct: 361 MVFGHGFLTKDGMKMGKSLGNTLEPFELVQKFGPDAVRYFFLREVEFGNDGDYSEDRFIK 420
Query: 421 IVNAHLANTIGNLLNRTLGLLKKNCQSTLVVDSTAAAEGNSFKDKVEELVLKARNHYEGL 480
IVNAHLANTIGNLLNRTLGLLKKNC+STLVVDST AAEG KD VE+LV KAR +YE L
Sbjct: 421 IVNAHLANTIGNLLNRTLGLLKKNCESTLVVDSTVAAEGVPLKDTVEKLVEKARTNYENL 480
Query: 481 SLSSACEAVLEIGNAGNSYMDEQAPWSLFKQGGASSDAAAKDLVLILEAMRIIAVALSPV 540
SLSSACEAVLEIGNAGN+YMD++APW LFKQGG S++ AAKDLV+ILE MR+IAVALSPV
Sbjct: 481 SLSSACEAVLEIGNAGNTYMDQRAPWFLFKQGGVSAEEAAKDLVIILEVMRVIAVALSPV 540
Query: 541 TPCLGWRIYAQLGFSKDQFEAATWNETKWGGLKAGQVMAPPNPVFARIEQSTGSDEDE-- 600
PCL RIY+QLG+++DQF + TW++TKWGGLK GQVM +PVFARIE + +EDE
Sbjct: 541 APCLSLRIYSQLGYTEDQFNSITWSDTKWGGLKGGQVMEQASPVFARIELNPEKEEDEKK 600
Query: 601 --VPKKPVKSKKKIAQ---GVAEA 617
V KK K+K K+ + VAEA
Sbjct: 601 PKVGKKTGKAKVKVVEQTPTVAEA 616
BLAST of Moc04g34620 vs. TAIR 10
Match:
AT3G55400.2 (methionyl-tRNA synthetase / methionine--tRNA ligase / MetRS (cpMetRS) )
HSP 1 Score: 803.1 bits (2073), Expect = 1.5e-232
Identity = 401/523 (76.67%), Postives = 449/523 (85.85%), Query Frame = 0
Query: 1 MAARINSSIQSSLWLLNPLQSSLNLNLNLNLNLRTRLNFYRNLLPSPSYSA-SSSKRAIF 60
MAARIN+S+ ++L L P + LN R N +R P YS SS KRA++
Sbjct: 1 MAARINTSLHNALSFLKPFNTPLNTK-----PFSFRRNSFRFSKKLPYYSQFSSGKRALY 60
Query: 61 CTYASNCNSHESGAATDPFVITTPLYYVNAAPHMGSAYTTIAADAIARFQRLLGKKVIFI 120
CT +S ++ + G + FV+TTPLYYVNA PHMGSAYTTIAAD+IARFQRLLGKKVIFI
Sbjct: 61 CTSSSQESTVDEG---ETFVLTTPLYYVNAPPHMGSAYTTIAADSIARFQRLLGKKVIFI 120
Query: 121 TGTDEHGEKIATAAAAQGSAPSEHCDAISEAYKTLWKDLDVAYDKFIRTTDPKHEAIVKE 180
TGTDEHGEKIAT+AAA G P EHCD IS++Y+TLWKDLD+AYDKFIRTTDPKHEAIVKE
Sbjct: 121 TGTDEHGEKIATSAAANGRNPPEHCDLISQSYRTLWKDLDIAYDKFIRTTDPKHEAIVKE 180
Query: 181 FYSKVLANGDIYRADYEGLYCVNCEEYKDEKELLANNCCPIHLKPCASRKEDNYFFALSK 240
FY++V ANGDIYRADYEGLYCVNCEEYKDEKELL NNCCP+H PC +RKEDNYFFALSK
Sbjct: 181 FYARVFANGDIYRADYEGLYCVNCEEYKDEKELLENNCCPVHQMPCVARKEDNYFFALSK 240
Query: 241 YQGLLEETLTQNPGFVQPAFRLNEVQSWIRSGLKDFSISRASVEWGIPVPNDQKQTIYVW 300
YQ LE+ L QNP FVQP++RLNEVQSWI+SGL+DFSISRA V+WGIPVP+D KQTIYVW
Sbjct: 241 YQKPLEDILAQNPRFVQPSYRLNEVQSWIKSGLRDFSISRALVDWGIPVPDDDKQTIYVW 300
Query: 301 FDALLGYISALSENGQQQNLQRAVASGWPASLHLIGKDILRFHAVYWPAMLMSAGLKLPK 360
FDALLGYISAL+E+ +QQNL+ AV+ GWPASLHLIGKDILRFHAVYWPAMLMSAGL+LPK
Sbjct: 301 FDALLGYISALTEDNKQQNLETAVSFGWPASLHLIGKDILRFHAVYWPAMLMSAGLELPK 360
Query: 361 MVFGHGFLTKDGMKMGKSLGNTLEPNELVQKFGSDAVRYFFLQEVEFGSDGDYAEDRFIN 420
MVFGHGFLTKDGMKMGKSLGNTLEP ELVQKFG DAVRYFFL+EVEFG+DGDY+EDRFI
Sbjct: 361 MVFGHGFLTKDGMKMGKSLGNTLEPFELVQKFGPDAVRYFFLREVEFGNDGDYSEDRFIK 420
Query: 421 IVNAHLANTIGNLLNRTLGLLKKNCQSTLVVDSTAAAEGNSFKDKVEELVLKARNHYEGL 480
IVNAHLANTIGNLLNRTLGLLKKNC+STLVVDST AAEG KD VE+LV KAR +YE L
Sbjct: 421 IVNAHLANTIGNLLNRTLGLLKKNCESTLVVDSTVAAEGVPLKDTVEKLVEKARTNYENL 480
Query: 481 SLSSACEAVLEIGNAGNSYMDEQAPWSLFKQGGASSDAAAKDL 523
SLSSACEAVLEIGNAGN+YMD++APW LFKQGG S++ AAK L
Sbjct: 481 SLSSACEAVLEIGNAGNTYMDQRAPWFLFKQGGVSAEEAAKVL 515
BLAST of Moc04g34620 vs. TAIR 10
Match:
AT4G13780.1 (methionine--tRNA ligase, putative / methionyl-tRNA synthetase, putative / MetRS, putative )
HSP 1 Score: 162.2 bits (409), Expect = 1.4e-39
Identity = 143/571 (25.04%), Postives = 238/571 (41.68%), Query Frame = 0
Query: 79 VITTPLYYVNAAPHMGSAY-TTIAADAIARFQRLLGKKVIFITGTDEHGEKIATAAAAQG 138
+IT+ L YVN PH+G+ ++AD AR+ RL G I+I GTDE+G T A +
Sbjct: 20 LITSALPYVNNVPHLGNIIGCVLSADVYARYCRLRGYNAIYICGTDEYGTATETKALEEN 79
Query: 139 SAPSEHCDAISEAYKTLWKDLDVAYDKFIRTTDPKHEAIVKEFYSKVLANGDIYRADYEG 198
P E CD +K ++ +++DKF RT+ P+ + + ++K+ N + +
Sbjct: 80 CTPKEICDKYHAIHKEVYDWFGISFDKFGRTSTPEQTEVCQAIFNKLWDNKWLSENTMQQ 139
Query: 199 LYCVNCEEYKDEKELLANNCCPIH----------------------------LKPCAS-- 258
LYC C+++ ++ L CP K C +
Sbjct: 140 LYCDTCKKFLADR--LVEGSCPFEGCNYDSARGDQCEKCGKLLNPTELKDPKCKVCQNTP 199
Query: 259 --RKEDNYFFALSKYQGLLEETLTQNPGFVQPAFRLNEVQ---SWIRSGLKDFSISRASV 318
R D+ F L + LE + + V ++ N +Q +W+R GL+ I+R +
Sbjct: 200 RIRDTDHLFIELPLLKDRLEAYIKKTS--VTGSWSQNAIQTTNAWLRDGLRQRCITR-DL 259
Query: 319 EWGIPVPND--QKQTIYVWFDALLGYISALSENGQQQNLQRAVASGW------PASLHL- 378
+WG+PVP++ + + YVWFDA +GY+S S S W P ++ L
Sbjct: 260 KWGVPVPHEKYKDKVFYVWFDAPIGYVSITS----------CYTSEWEKWWKNPENVELY 319
Query: 379 --IGKDILRFHAVYWPAMLMSA--GLKLPKMVFGHGFLTKDGMKMGKSLGNTLEPNELVQ 438
+GKD + FH V +P+ + L K + +L + K KS G + N++
Sbjct: 320 QFMGKDNVPFHTVMFPSTQLGTEENWTLMKTISVTEYLNYEDGKFSKSKGVGVFGNDVKD 379
Query: 439 -KFGSDAVRYFFLQEVEFGSDGDYAEDRFINIVNAHLANTIGNLLNRTLGLLKK--NCQS 498
+ RY+ L SD ++ +N L + +GN +NR L + K N
Sbjct: 380 TNIPVEVWRYYLLTNRPEVSDTSFSWTDLQAKLNGELLSNLGNFVNRVLSFIAKPDNAGY 439
Query: 499 TLVVDSTAAAEGN----SFKDKVEELVLKARNHYEGLSLSSACEAVLEIGNAGNSYMDEQ 558
V+ AE + S +KVE+ V + E + L + + I + GN Y+
Sbjct: 440 GSVIPDAHDAESHSLTKSLAEKVEKFVAEYVEAMEKVKLKQGLKTAMLISSEGNYYLQAS 499
Query: 559 APWSLFKQGGASSDAAAKDLVLILEAMRIIAVALSPVTPCLGWRIYAQLG----FS-KDQ 589
W L+K+ A + + ++A L P P ++ QL FS D+
Sbjct: 500 QFWKLYKE---DKPLCAVVIRTAAGLVHLLAQLLEPFMPSFSCEVFKQLNLPPQFSLSDE 559
BLAST of Moc04g34620 vs. TAIR 10
Match:
AT5G16715.1 (ATP binding;valine-tRNA ligases;aminoacyl-tRNA ligases;nucleotide binding;ATP binding;aminoacyl-tRNA ligases )
HSP 1 Score: 48.9 bits (115), Expect = 1.7e-05
Identity = 33/106 (31.13%), Postives = 52/106 (49.06%), Query Frame = 0
Query: 299 WFDALLGYISALSENGQQQNLQRAVASGWPASLHLIGKDILRFHAVYWPAMLMSAGLKLP 358
WF + L S L G + + +P ++ G DIL F W A ++ G++
Sbjct: 525 WFSSSLWPFSTL---GWPDVAAKDFNNFYPTNMLETGHDILFF----WVARMVMMGIEFT 584
Query: 359 KMV-FGHGFL-----TKDGMKMGKSLGNTLEPNELVQKFGSDAVRY 399
V F H +L G KM KSLGN ++P + ++ FG+DA+R+
Sbjct: 585 GTVPFSHVYLHGLIRDSQGRKMSKSLGNVIDPLDTIKDFGTDALRF 623
BLAST of Moc04g34620 vs. TAIR 10
Match:
AT5G49030.1 (tRNA synthetase class I (I, L, M and V) family protein )
HSP 1 Score: 47.8 bits (112), Expect = 3.8e-05
Identity = 78/306 (25.49%), Postives = 123/306 (40.20%), Query Frame = 0
Query: 295 TIYVWFDALLGYISALSENGQQQNLQRAVASGWPASLHLIGKDILRFHAVYWPAMLMSA- 354
T+ VWFD+ + L G+++ L +PA ++L G D H ++ + L+++
Sbjct: 652 TMDVWFDSGSSWAGVL---GKREGL------SFPADVYLEGTD---QHRGWFQSSLLTSI 711
Query: 355 ---GLKLPKMVFGHGF-LTKDGMKMGKSLGNTLEPNELVQ--KFGSDAVRYFFLQEVEFG 414
G V HGF L + GMKM KSLGN ++P +++ K DA Y +
Sbjct: 712 ATQGKAPYSAVITHGFVLDEKGMKMSKSLGNVVDPRLVIEGGKNSKDAPAYGADVMRLWV 771
Query: 415 SDGDYAEDRFINIVNAHLANTIGNLLNRTLGLLKKNCQSTLVVDSTAAAEGNSFKD---- 474
S DY D I + I L TL L N VD+ + D
Sbjct: 772 SSVDYTGDVLIGPQILRQMSDIYRKLRGTLRYLLGNLHDWR-VDNAVPYQDLPIIDQHAL 831
Query: 475 -KVEELVLKARNHYEGLSLSSACEAV--LEIGNAGNSYMDEQAPWSLFKQGGASSDAAAK 534
++E +V + YE + + I + N Y D A L+ G +S +
Sbjct: 832 FQLENVVKNIQECYENYQFFKIFQIIQRFTIVDLSNFYFD-IAKDRLYTGGTSSFTRRSC 891
Query: 535 DLVLILEAMRIIAVALSPVTPCLGWRIYAQLGF---SKDQFEAATWNETKWGGLKAGQVM 584
VL + I+ V ++P+ P L ++ L F ++D A E KW L +
Sbjct: 892 QTVLSTHLLSILRV-IAPIVPHLAEDVWQNLPFEYRNEDGSAAEFVFELKWPTLNEQWLS 942
The following BLAST results are available for this feature:
Match Name | E-value | Identity | Description | |
XP_022133974.1 | 0.0e+00 | 100.00 | methionine--tRNA ligase, chloroplastic/mitochondrial [Momordica charantia] | [more] |
XP_038891037.1 | 0.0e+00 | 90.42 | methionine--tRNA ligase, chloroplastic/mitochondrial [Benincasa hispida] | [more] |
XP_004142742.1 | 0.0e+00 | 90.42 | methionine--tRNA ligase, chloroplastic/mitochondrial [Cucumis sativus] >KGN51284... | [more] |
KAA0066020.1 | 0.0e+00 | 90.42 | methionine--tRNA ligase [Cucumis melo var. makuwa] >TYK05823.1 methionine--tRNA ... | [more] |
XP_022960433.1 | 0.0e+00 | 90.44 | methionine--tRNA ligase, chloroplastic/mitochondrial [Cucurbita moschata] | [more] |
Match Name | E-value | Identity | Description | |
Q9M2T9 | 5.6e-264 | 73.88 | Methionine--tRNA ligase, chloroplastic/mitochondrial OS=Arabidopsis thaliana OX=... | [more] |
Q55729 | 1.9e-158 | 52.98 | Methionine--tRNA ligase OS=Synechocystis sp. (strain PCC 6803 / Kazusa) OX=11117... | [more] |
Q8Z068 | 3.5e-157 | 52.96 | Methionine--tRNA ligase OS=Nostoc sp. (strain PCC 7120 / SAG 25.82 / UTEX 2576) ... | [more] |
P59081 | 1.6e-149 | 50.77 | Methionine--tRNA ligase OS=Thermosynechococcus elongatus (strain BP-1) OX=197221... | [more] |
O67298 | 1.3e-119 | 46.57 | Methionine--tRNA ligase OS=Aquifex aeolicus (strain VF5) OX=224324 GN=metG PE=1 ... | [more] |
Match Name | E-value | Identity | Description | |
A0A6J1BXG8 | 0.0e+00 | 100.00 | Methionine--tRNA ligase OS=Momordica charantia OX=3673 GN=LOC111006379 PE=3 SV=1 | [more] |
A0A0A0KQV7 | 0.0e+00 | 90.42 | Methionine--tRNA ligase OS=Cucumis sativus OX=3659 GN=Csa_5G512910 PE=3 SV=1 | [more] |
A0A5D3C1K0 | 0.0e+00 | 90.42 | Methionine--tRNA ligase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold... | [more] |
A0A6J1H8X3 | 0.0e+00 | 90.44 | Methionine--tRNA ligase OS=Cucurbita moschata OX=3662 GN=LOC111461165 PE=3 SV=1 | [more] |
A0A1S3CJS7 | 0.0e+00 | 90.42 | Methionine--tRNA ligase OS=Cucumis melo OX=3656 GN=LOC103501636 PE=3 SV=1 | [more] |
Match Name | E-value | Identity | Description | |
AT3G55400.1 | 4.0e-265 | 73.88 | methionyl-tRNA synthetase / methionine--tRNA ligase / MetRS (cpMetRS) | [more] |
AT3G55400.2 | 1.5e-232 | 76.67 | methionyl-tRNA synthetase / methionine--tRNA ligase / MetRS (cpMetRS) | [more] |
AT4G13780.1 | 1.4e-39 | 25.04 | methionine--tRNA ligase, putative / methionyl-tRNA synthetase, putative / MetRS,... | [more] |
AT5G16715.1 | 1.7e-05 | 31.13 | ATP binding;valine-tRNA ligases;aminoacyl-tRNA ligases;nucleotide binding;ATP bi... | [more] |
AT5G49030.1 | 3.8e-05 | 25.49 | tRNA synthetase class I (I, L, M and V) family protein | [more] |