Moc04g34600 (gene) Bitter gourd (OHB3-1) v2

Overview


Name	Moc04g34600
Type	gene
Organism	Momordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
Description	enoyl-CoA delta isomerase 1, peroxisomal
Location	chr4: 26102515 .. 26103225 (-)
RNA-Seq Expression	Moc04g34600
Synteny	Moc04g34600

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGTGCTCGTTACAGAGGCGAGGGAATGTCTTTCTCCTAACCCTAACCGGTCCAGGCGAGCACAGACTCAATCCCACGCTTATAGATGCGATTAAATCCTCTCTCAGTCAAGTCCGTTCCGAGGCTGAATCCGCCGCCGGTAATCCATCTAGTTTTGCCCTAATCACCACCGCCGAAGGCAAATTCTTCTCCAATGGCTACGATATCGATTGGGCTGCGTCCTCACTTGATCGGGAGTATCTCATGGACGATAAGTTAAAATCCGTCGCATCCGATCTCATCACGCTTCCGATGCCGACGATTGCCGCCGTTTCCGGCCACGCGTCGGCCGCCGGCTTCGTATTGGCGATGAGTCACGACTACATCCTCATGAGGAGAGATCGGGGATTCCTGTACATGAGCGAACTCGACATTCGCCGCGTGATTCCGTCTTGGTTTATGGCTCTGGTGAAGAACAAAATCGGAGCGCCGGTGGCTCGACGCGACGTCGTTTTGAAAGCGCCTAAACTGACGGCGGATAAGGCTCTGGAAATGGGGATTATTGACTCGGCGCACGATAGCGCGGCAGAGACCGTCGCGGCTGCCGTTCGATTGGGCGAGGAATTGGTGGCGAGGAATTGGGACGGTCCGATTTACGCCCGGAATCGGGTCGAGCTGCTCTCTGAAGTTCTGAACGTCATCGGGACACGCTGCATCGTCGCGAAGCTTTAG

mRNA sequence

ATGTGCTCGTTACAGAGGCGAGGGAATGTCTTTCTCCTAACCCTAACCGGTCCAGGCGAGCACAGACTCAATCCCACGCTTATAGATGCGATTAAATCCTCTCTCAGTCAAGTCCGTTCCGAGGCTGAATCCGCCGCCGGTAATCCATCTAGTTTTGCCCTAATCACCACCGCCGAAGGCAAATTCTTCTCCAATGGCTACGATATCGATTGGGCTGCGTCCTCACTTGATCGGGAGTATCTCATGGACGATAAGTTAAAATCCGTCGCATCCGATCTCATCACGCTTCCGATGCCGACGATTGCCGCCGTTTCCGGCCACGCGTCGGCCGCCGGCTTCGTATTGGCGATGAGTCACGACTACATCCTCATGAGGAGAGATCGGGGATTCCTGTACATGAGCGAACTCGACATTCGCCGCGTGATTCCGTCTTGGTTTATGGCTCTGGTGAAGAACAAAATCGGAGCGCCGGTGGCTCGACGCGACGTCGTTTTGAAAGCGCCTAAACTGACGGCGGATAAGGCTCTGGAAATGGGGATTATTGACTCGGCGCACGATAGCGCGGCAGAGACCGTCGCGGCTGCCGTTCGATTGGGCGAGGAATTGGTGGCGAGGAATTGGGACGGTCCGATTTACGCCCGGAATCGGGTCGAGCTGCTCTCTGAAGTTCTGAACGTCATCGGGACACGCTGCATCGTCGCGAAGCTTTAG

Coding sequence (CDS)

ATGTGCTCGTTACAGAGGCGAGGGAATGTCTTTCTCCTAACCCTAACCGGTCCAGGCGAGCACAGACTCAATCCCACGCTTATAGATGCGATTAAATCCTCTCTCAGTCAAGTCCGTTCCGAGGCTGAATCCGCCGCCGGTAATCCATCTAGTTTTGCCCTAATCACCACCGCCGAAGGCAAATTCTTCTCCAATGGCTACGATATCGATTGGGCTGCGTCCTCACTTGATCGGGAGTATCTCATGGACGATAAGTTAAAATCCGTCGCATCCGATCTCATCACGCTTCCGATGCCGACGATTGCCGCCGTTTCCGGCCACGCGTCGGCCGCCGGCTTCGTATTGGCGATGAGTCACGACTACATCCTCATGAGGAGAGATCGGGGATTCCTGTACATGAGCGAACTCGACATTCGCCGCGTGATTCCGTCTTGGTTTATGGCTCTGGTGAAGAACAAAATCGGAGCGCCGGTGGCTCGACGCGACGTCGTTTTGAAAGCGCCTAAACTGACGGCGGATAAGGCTCTGGAAATGGGGATTATTGACTCGGCGCACGATAGCGCGGCAGAGACCGTCGCGGCTGCCGTTCGATTGGGCGAGGAATTGGTGGCGAGGAATTGGGACGGTCCGATTTACGCCCGGAATCGGGTCGAGCTGCTCTCTGAAGTTCTGAACGTCATCGGGACACGCTGCATCGTCGCGAAGCTTTAG

Protein sequence

MCSLQRRGNVFLLTLTGPGEHRLNPTLIDAIKSSLSQVRSEAESAAGNPSSFALITTAEGKFFSNGYDIDWAASSLDREYLMDDKLKSVASDLITLPMPTIAAVSGHASAAGFVLAMSHDYILMRRDRGFLYMSELDIRRVIPSWFMALVKNKIGAPVARRDVVLKAPKLTADKALEMGIIDSAHDSAAETVAAAVRLGEELVARNWDGPIYARNRVELLSEVLNVIGTRCIVAKL

Homology

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BLAST of Moc04g34600 vs. NCBI nr
Match: XP_022133536.1 (enoyl-CoA delta isomerase 1, peroxisomal [Momordica charantia])

HSP 1 Score: 455.3 bits (1170), Expect = 3.2e-124
Identity = 236/236 (100.00%), Postives = 236/236 (100.00%), Query Frame = 0


Query: 1   MCSLQRRGNVFLLTLTGPGEHRLNPTLIDAIKSSLSQVRSEAESAAGNPSSFALITTAEG 60
           MCSLQRRGNVFLLTLTGPGEHRLNPTLIDAIKSSLSQVRSEAESAAGNPSSFALITTAEG
Sbjct: 1   MCSLQRRGNVFLLTLTGPGEHRLNPTLIDAIKSSLSQVRSEAESAAGNPSSFALITTAEG 60

Query: 61  KFFSNGYDIDWAASSLDREYLMDDKLKSVASDLITLPMPTIAAVSGHASAAGFVLAMSHD 120
           KFFSNGYDIDWAASSLDREYLMDDKLKSVASDLITLPMPTIAAVSGHASAAGFVLAMSHD
Sbjct: 61  KFFSNGYDIDWAASSLDREYLMDDKLKSVASDLITLPMPTIAAVSGHASAAGFVLAMSHD 120

Query: 121 YILMRRDRGFLYMSELDIRRVIPSWFMALVKNKIGAPVARRDVVLKAPKLTADKALEMGI 180
           YILMRRDRGFLYMSELDIRRVIPSWFMALVKNKIGAPVARRDVVLKAPKLTADKALEMGI
Sbjct: 121 YILMRRDRGFLYMSELDIRRVIPSWFMALVKNKIGAPVARRDVVLKAPKLTADKALEMGI 180

Query: 181 IDSAHDSAAETVAAAVRLGEELVARNWDGPIYARNRVELLSEVLNVIGTRCIVAKL 237
           IDSAHDSAAETVAAAVRLGEELVARNWDGPIYARNRVELLSEVLNVIGTRCIVAKL
Sbjct: 181 IDSAHDSAAETVAAAVRLGEELVARNWDGPIYARNRVELLSEVLNVIGTRCIVAKL 236

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BLAST of Moc04g34600 vs. NCBI nr
Match: KAG6578713.1 (Enoyl-CoA delta isomerase 1, peroxisomal, partial [Cucurbita argyrosperma subsp. sororia] >KAG7016244.1 Enoyl-CoA delta isomerase 1, peroxisomal, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 383.6 bits (984), Expect = 1.2e-102
Identity = 200/238 (84.03%), Postives = 214/238 (89.92%), Query Frame = 0


Query: 1   MCSLQRRGNVFLLTLTGPGEHRLNPTLIDAIKSSLSQVRSEAESA--AGNPSSFALITTA 60
           MCSLQRRGN+FLLTLTGPGEHRLN  L+DAI+SS+ QVRSEAESA  AGN +SFALITTA
Sbjct: 1   MCSLQRRGNIFLLTLTGPGEHRLNLNLLDAIQSSVRQVRSEAESAVVAGNSASFALITTA 60

Query: 61  EGKFFSNGYDIDWAASSLDREYLMDDKLKSVASDLITLPMPTIAAVSGHASAAGFVLAMS 120
           EGKFFSNGYDIDWA +S+D+  LMDDKLKSV SDLITLPMPTIAAV+GHASAAGF+ AMS
Sbjct: 61  EGKFFSNGYDIDWAKTSMDKMILMDDKLKSVVSDLITLPMPTIAAVTGHASAAGFLFAMS 120

Query: 121 HDYILMRRDRGFLYMSELDIRRVIPSWFMALVKNKIGAPVARRDVVLKAPKLTADKALEM 180
           HDYILMRRDRGFLYMSELDIRRVIPSWF  LVK KIGA   RRDVVLKAPKLTADKALEM
Sbjct: 121 HDYILMRRDRGFLYMSELDIRRVIPSWFTVLVKGKIGAASVRRDVVLKAPKLTADKALEM 180

Query: 181 GIIDSAHDSAAETVAAAVRLGEELVARNWDGPIYARNRVELLSEVLNVIGTRCIVAKL 237
           GII+SAHD AAETVAAAV+L E+LVAR WDG IYA NR+ELLSEVLNVIG RC VAKL
Sbjct: 181 GIIESAHDGAAETVAAAVKLAEDLVARKWDGHIYAGNRMELLSEVLNVIGARCSVAKL 238

[Close]

BLAST of Moc04g34600 vs. NCBI nr
Match: XP_023550952.1 (enoyl-CoA delta isomerase 1, peroxisomal [Cucurbita pepo subsp. pepo])

HSP 1 Score: 382.9 bits (982), Expect = 2.0e-102
Identity = 198/238 (83.19%), Postives = 215/238 (90.34%), Query Frame = 0


Query: 1   MCSLQRRGNVFLLTLTGPGEHRLNPTLIDAIKSSLSQVRSEAESA--AGNPSSFALITTA 60
           MCSLQRRGN+FLLTLTGPGEHRLN  L+DAI+SS+ QVRSEAESA  AGN +SFALITTA
Sbjct: 1   MCSLQRRGNIFLLTLTGPGEHRLNLNLLDAIQSSVRQVRSEAESAVVAGNSASFALITTA 60

Query: 61  EGKFFSNGYDIDWAASSLDREYLMDDKLKSVASDLITLPMPTIAAVSGHASAAGFVLAMS 120
           EGKFFSNGYD+DWA +S+D+  LMDDKLKSV SDLITLPMPTIAAV+GHASAAGF+LAMS
Sbjct: 61  EGKFFSNGYDLDWAKTSMDKMILMDDKLKSVVSDLITLPMPTIAAVTGHASAAGFILAMS 120

Query: 121 HDYILMRRDRGFLYMSELDIRRVIPSWFMALVKNKIGAPVARRDVVLKAPKLTADKALEM 180
           HDYILMRRDRGFLYMSELDIRRVIPSWF  LVK+KIG    RRDVVLKAPKLTADKALEM
Sbjct: 121 HDYILMRRDRGFLYMSELDIRRVIPSWFTVLVKSKIGTASVRRDVVLKAPKLTADKALEM 180

Query: 181 GIIDSAHDSAAETVAAAVRLGEELVARNWDGPIYARNRVELLSEVLNVIGTRCIVAKL 237
           GII+SAHD AAETVAAAV+L E++VAR WDG IYA NR+ELLSEVLNVIG RC VAKL
Sbjct: 181 GIIESAHDGAAETVAAAVKLAEDVVARKWDGHIYAGNRMELLSEVLNVIGARCSVAKL 238

[Close]

BLAST of Moc04g34600 vs. NCBI nr
Match: XP_022939279.1 (enoyl-CoA delta isomerase 1, peroxisomal [Cucurbita moschata])

HSP 1 Score: 382.1 bits (980), Expect = 3.5e-102
Identity = 199/238 (83.61%), Postives = 213/238 (89.50%), Query Frame = 0


Query: 1   MCSLQRRGNVFLLTLTGPGEHRLNPTLIDAIKSSLSQVRSEAESA--AGNPSSFALITTA 60
           MCSLQRRGN+FLLTLTGPGEHRLN  L+DAI+SS+ QVRSEAESA  AGN +SFALITTA
Sbjct: 1   MCSLQRRGNIFLLTLTGPGEHRLNLNLLDAIQSSVRQVRSEAESAVVAGNSASFALITTA 60

Query: 61  EGKFFSNGYDIDWAASSLDREYLMDDKLKSVASDLITLPMPTIAAVSGHASAAGFVLAMS 120
           EGKFFSNGYDIDWA SS+D+  LMDDKLKSV SDLITLPMPTIAAV+GHASAAGF+ AMS
Sbjct: 61  EGKFFSNGYDIDWAKSSMDKMILMDDKLKSVVSDLITLPMPTIAAVTGHASAAGFLFAMS 120

Query: 121 HDYILMRRDRGFLYMSELDIRRVIPSWFMALVKNKIGAPVARRDVVLKAPKLTADKALEM 180
           HDYILMRRDRGFLYMSELDIRRVIPSWF  LVK+KIG    RRDVVLKAPKLTAD+ALEM
Sbjct: 121 HDYILMRRDRGFLYMSELDIRRVIPSWFTVLVKSKIGTASVRRDVVLKAPKLTADRALEM 180

Query: 181 GIIDSAHDSAAETVAAAVRLGEELVARNWDGPIYARNRVELLSEVLNVIGTRCIVAKL 237
           GII+SAHD AAETV AAV+L EELVAR WDG IYA NR+ELLSEVLNVIG RC VAKL
Sbjct: 181 GIIESAHDGAAETVTAAVKLAEELVARKWDGHIYAGNRMELLSEVLNVIGARCSVAKL 238

[Close]

BLAST of Moc04g34600 vs. NCBI nr
Match: XP_022993099.1 (enoyl-CoA delta isomerase 1, peroxisomal [Cucurbita maxima])

HSP 1 Score: 378.6 bits (971), Expect = 3.8e-101
Identity = 198/238 (83.19%), Postives = 214/238 (89.92%), Query Frame = 0


Query: 1   MCSLQRRGNVFLLTLTGPGEHRLNPTLIDAIKSSLSQVRSEAESA--AGNPSSFALITTA 60
           MCSLQRRGN+FLLTLTG GEHRLN  L+DAI+SS+ QVRSEAESA  AG  +SFALITTA
Sbjct: 1   MCSLQRRGNIFLLTLTGSGEHRLNLNLLDAIQSSVRQVRSEAESAVVAGKSASFALITTA 60

Query: 61  EGKFFSNGYDIDWAASSLDREYLMDDKLKSVASDLITLPMPTIAAVSGHASAAGFVLAMS 120
           EGKFFSNGYDIDWA +S+D+  LMDDKLKSV SDLITLPMPTIAAV+GHASAAGF+LAMS
Sbjct: 61  EGKFFSNGYDIDWAKTSMDKMILMDDKLKSVVSDLITLPMPTIAAVTGHASAAGFLLAMS 120

Query: 121 HDYILMRRDRGFLYMSELDIRRVIPSWFMALVKNKIGAPVARRDVVLKAPKLTADKALEM 180
           HDYILMRRDRGFLYMSELDIRRVIPSWF  LVK+KIGA   RRDVVLKAPKLTAD+ALEM
Sbjct: 121 HDYILMRRDRGFLYMSELDIRRVIPSWFTVLVKSKIGAASVRRDVVLKAPKLTADEALEM 180

Query: 181 GIIDSAHDSAAETVAAAVRLGEELVARNWDGPIYARNRVELLSEVLNVIGTRCIVAKL 237
           GII+SAHD AAETVAAAV+L E+LVAR WDG IYA NR+ELLSEVLNVIG RC VAKL
Sbjct: 181 GIIESAHDGAAETVAAAVKLAEDLVARKWDGHIYAGNRMELLSEVLNVIGARCSVAKL 238

[Close]

BLAST of Moc04g34600 vs. ExPASy Swiss-Prot
Match: O04469 (Enoyl-CoA delta isomerase 1, peroxisomal OS=Arabidopsis thaliana OX=3702 GN=ECI1 PE=1 SV=1)

HSP 1 Score: 274.6 bits (701), Expect = 1.0e-72
Identity = 136/224 (60.71%), Postives = 179/224 (79.91%), Query Frame = 0


Query: 1   MCSLQRRGNVFLLTLTGPGEHRLNPTLIDAIKSSLSQVRSEAESAAGNPSSFALITTAEG 60
           MCSL++R  +F+L LTG GEHRLNPTL+D+++S+++Q+RS+        S   LITT++G
Sbjct: 1   MCSLEKRDRLFILKLTGDGEHRLNPTLLDSLRSTINQIRSDPSF-----SQSVLITTSDG 60

Query: 61  KFFSNGYDIDWAASSLDREYLMDDKLKSVASDLITLPMPTIAAVSGHASAAGFVLAMSHD 120
           KFFSNGYD+  A S+     +MD KL+S+ +DLI+LPMPTIAAV+GHASAAG +LAMSHD
Sbjct: 61  KFFSNGYDLALAESNPSLSVVMDAKLRSLVADLISLPMPTIAAVTGHASAAGCILAMSHD 120

Query: 121 YILMRRDRGFLYMSELDIRRVIPSWFMALVKNKIGAPVARRDVVLKAPKLTADKALEMGI 180
           Y+LMRRDRGFLYMSELDI  ++P+WFMA+++ KIG+P ARRDV+L A K+TAD  ++MGI
Sbjct: 121 YVLMRRDRGFLYMSELDIELIVPAWFMAVIRGKIGSPAARRDVMLTAAKVTADVGVKMGI 180

Query: 181 IDSAHDSAAETVAAAVRLGEELVARNWDGPIYARNRVELLSEVL 225
           +DSA+ SAAETV AA++LGEE+V R  DG +Y + R  LL EVL
Sbjct: 181 VDSAYGSAAETVEAAIKLGEEIVQRGGDGHVYGKMRESLLREVL 219

[Close]

BLAST of Moc04g34600 vs. ExPASy Swiss-Prot
Match: O23299 (Enoyl-CoA delta isomerase 2, peroxisomal OS=Arabidopsis thaliana OX=3702 GN=ECI2 PE=1 SV=1)

HSP 1 Score: 215.3 bits (547), Expect = 7.4e-55
Identity = 113/235 (48.09%), Postives = 159/235 (67.66%), Query Frame = 0


Query: 1   MCSLQRRGNVFLLTLTGPGEHRLNPTLIDAIKSSLSQVRSEAESAAGNPSSFALITTAEG 60
           MC+L++RG++FLLTLTG GEHR +P  I  I S L Q +S++   +       LITTA G
Sbjct: 1   MCTLEKRGDLFLLTLTGDGEHRFHPDTIATILSLLEQAKSQSTRGS------ILITTANG 60

Query: 61  KFFSNGYDIDWA------ASSLDREYLMDDKLKSVASDLITLPMPTIAAVSGHASAAGFV 120
           KFFSNG+D+ WA        + +R + M +  K V + L+ LPMPTIAA++GHA+AAG +
Sbjct: 61  KFFSNGFDLAWAQTAGSKTGAANRLHQMVESFKPVVAALLDLPMPTIAALNGHAAAAGLI 120

Query: 121 LAMSHDYILMRRDRGFLYMSELDIRRVIPSWFMALVKNKIGAPVARRDVVLKAPKLTADK 180
           LA+SHDY+ MR+DRG LYMSE+DI   +P +F ALV+ KIG   ARR+++L   K+  ++
Sbjct: 121 LALSHDYVFMRKDRGVLYMSEVDIGLSMPDYFSALVRAKIGTSAARRELLLSGKKIRGEE 180

Query: 181 ALEMGIIDS-AHDSAAETVAAAVRLGEELVARNWDGPIYARNRVELLSEVLNVIG 229
           A+ +GI+DS A+DS    V A+VRLGE+L A+ W G +YA  R  L  E+  ++G
Sbjct: 181 AVGLGIVDSAAYDSEEGVVVASVRLGEKLAAKKWSGEVYASIRKSLYPELCGILG 229

[Close]

BLAST of Moc04g34600 vs. ExPASy Swiss-Prot
Match: O23300 (Enoyl-CoA delta isomerase 3 OS=Arabidopsis thaliana OX=3702 GN=ECI3 PE=1 SV=1)

HSP 1 Score: 209.1 bits (531), Expect = 5.3e-53
Identity = 109/232 (46.98%), Postives = 156/232 (67.24%), Query Frame = 0


Query: 1   MCSLQRRGNVFLLTLTGPGEHRLNPTLIDAIKSSLSQVRSEAESAAGNPSSFALITTAEG 60
           MC+L++RG++FLLTLTG  EHR +P  I ++ S L Q +S++   +       LITT  G
Sbjct: 1   MCTLEKRGDLFLLTLTGEDEHRFHPDTIASVLSLLEQAKSQSTKGS------VLITTGHG 60

Query: 61  KFFSNGYDIDWAAS----SLDREYLMDDKLKSVASDLITLPMPTIAAVSGHASAAGFVLA 120
           KFFSNG+D+ WA S    ++ R + M    K V + L+ LPMPTIAA++GHA+A+G + A
Sbjct: 61  KFFSNGFDLAWAQSAGHGAIKRMHQMVKSFKPVLAALLDLPMPTIAALNGHAAASGLMFA 120

Query: 121 MSHDYILMRRDRGFLYMSELDIRRVIPSWFMALVKNKIGAPVARRDVVLKAPKLTADKAL 180
           +SHDY+ MR+DRG LYMSE+DI   +P +F ALV  K+G+ +ARR+++L   KL  ++A+
Sbjct: 121 LSHDYVFMRKDRGVLYMSEVDIGLPVPDYFSALVVAKVGSGIARRELLLSGKKLKGEEAV 180

Query: 181 EMGIIDS-AHDSAAETVAAAVRLGEELVARNWDGPIYARNRVELLSEVLNVI 228
            +GI+DS AHDSA   V A V LGE L A+ W+G +YA  R  L  E+  ++
Sbjct: 181 ALGIVDSAAHDSAEGVVEATVSLGESLAAKKWNGEVYATIRKSLYPELCRMV 226

[Close]

BLAST of Moc04g34600 vs. ExPASy Swiss-Prot
Match: Q8Z9L5 (Carnitinyl-CoA dehydratase OS=Salmonella typhi OX=90370 GN=caiD PE=3 SV=3)

HSP 1 Score: 56.2 bits (134), Expect = 5.7e-07
Identity = 52/200 (26.00%), Postives = 95/200 (47.50%), Query Frame = 0


Query: 4   LQRRGNVFLLTLTGPGEHRLNPTLIDAIKSSLSQVRSEAESAAGNPSSFALITTAEGKFF 63
           L R G +  +TL  P  + ++     A+  +    R + E         A+IT    KFF
Sbjct: 7   LTRNGPILEITLDRPKANAIDAKTSFAMGEAFLNFRDDPELRV------AIITGGGEKFF 66

Query: 64  SNGYDIDWAASSLDREYLMDDKLKSVASDLITLPMPTIAAVSGHASAAGFVLAMSHDYIL 123
           S G+D+  AA     +        +  +++  L  P IAAV+G+A   GF LA++ D+I+
Sbjct: 67  SAGWDLKAAAEGEAPDADFGPGGFAGLTEIFDLDKPVIAAVNGYAFGGGFELALAADFIV 126

Query: 124 MRRDRGFLYMSELDIRRVIPSWFMALVKNKIGAPVARRDVVLKAPKLTADKALEMGIIDS 183
              +  F  + E  +  ++P     L   K+  P    ++V+   +++A++AL  GI++ 
Sbjct: 127 CAENASFA-LPEAKL-GIVPDSGGVLRLPKLLPPAIVNEMVMTGRRMSAEEALRWGIVNR 186

Query: 184 AHDSAAETVAAAVRLGEELV 204
              S +E + +A  L ++LV
Sbjct: 187 V-VSQSELMESARELAQQLV 197

[Close]

BLAST of Moc04g34600 vs. ExPASy Swiss-Prot
Match: B5F749 (Carnitinyl-CoA dehydratase OS=Salmonella agona (strain SL483) OX=454166 GN=caiD PE=3 SV=1)

HSP 1 Score: 55.8 bits (133), Expect = 7.5e-07
Identity = 52/200 (26.00%), Postives = 95/200 (47.50%), Query Frame = 0


Query: 4   LQRRGNVFLLTLTGPGEHRLNPTLIDAIKSSLSQVRSEAESAAGNPSSFALITTAEGKFF 63
           L R G +  +TL  P  + ++     A+  +    R + E         A+IT    KFF
Sbjct: 7   LTRNGPILEITLDRPKANAIDAKTSFAMGEAFLNFRDDPELRV------AIITGGGEKFF 66

Query: 64  SNGYDIDWAASSLDREYLMDDKLKSVASDLITLPMPTIAAVSGHASAAGFVLAMSHDYIL 123
           S G+D+  AA     +        +  +++  L  P IAAV+G+A   GF LA++ D+I+
Sbjct: 67  SAGWDLKAAAEGEAPDADFGPGGFAGLTEIFDLDKPVIAAVNGYAFGGGFELALAADFIV 126

Query: 124 MRRDRGFLYMSELDIRRVIPSWFMALVKNKIGAPVARRDVVLKAPKLTADKALEMGIIDS 183
              +  F  + E  +  ++P     L   K+  P    ++V+   +++A++AL  GI++ 
Sbjct: 127 CAENASFA-LPEAKL-GIVPDSGGVLRLPKLLPPAIVNEMVMTGRRMSAEEALRWGIVNR 186

Query: 184 AHDSAAETVAAAVRLGEELV 204
              S +E + +A  L ++LV
Sbjct: 187 V-VSQSELMDSARELAQQLV 197

[Close]

BLAST of Moc04g34600 vs. ExPASy TrEMBL
Match: A0A6J1BVD7 (enoyl-CoA delta isomerase 1, peroxisomal OS=Momordica charantia OX=3673 GN=LOC111006096 PE=4 SV=1)

HSP 1 Score: 455.3 bits (1170), Expect = 1.6e-124
Identity = 236/236 (100.00%), Postives = 236/236 (100.00%), Query Frame = 0


Query: 1   MCSLQRRGNVFLLTLTGPGEHRLNPTLIDAIKSSLSQVRSEAESAAGNPSSFALITTAEG 60
           MCSLQRRGNVFLLTLTGPGEHRLNPTLIDAIKSSLSQVRSEAESAAGNPSSFALITTAEG
Sbjct: 1   MCSLQRRGNVFLLTLTGPGEHRLNPTLIDAIKSSLSQVRSEAESAAGNPSSFALITTAEG 60

Query: 61  KFFSNGYDIDWAASSLDREYLMDDKLKSVASDLITLPMPTIAAVSGHASAAGFVLAMSHD 120
           KFFSNGYDIDWAASSLDREYLMDDKLKSVASDLITLPMPTIAAVSGHASAAGFVLAMSHD
Sbjct: 61  KFFSNGYDIDWAASSLDREYLMDDKLKSVASDLITLPMPTIAAVSGHASAAGFVLAMSHD 120

Query: 121 YILMRRDRGFLYMSELDIRRVIPSWFMALVKNKIGAPVARRDVVLKAPKLTADKALEMGI 180
           YILMRRDRGFLYMSELDIRRVIPSWFMALVKNKIGAPVARRDVVLKAPKLTADKALEMGI
Sbjct: 121 YILMRRDRGFLYMSELDIRRVIPSWFMALVKNKIGAPVARRDVVLKAPKLTADKALEMGI 180

Query: 181 IDSAHDSAAETVAAAVRLGEELVARNWDGPIYARNRVELLSEVLNVIGTRCIVAKL 237
           IDSAHDSAAETVAAAVRLGEELVARNWDGPIYARNRVELLSEVLNVIGTRCIVAKL
Sbjct: 181 IDSAHDSAAETVAAAVRLGEELVARNWDGPIYARNRVELLSEVLNVIGTRCIVAKL 236

[Close]

BLAST of Moc04g34600 vs. ExPASy TrEMBL
Match: A0A6J1FGP4 (enoyl-CoA delta isomerase 1, peroxisomal OS=Cucurbita moschata OX=3662 GN=LOC111445244 PE=4 SV=1)

HSP 1 Score: 382.1 bits (980), Expect = 1.7e-102
Identity = 199/238 (83.61%), Postives = 213/238 (89.50%), Query Frame = 0


Query: 1   MCSLQRRGNVFLLTLTGPGEHRLNPTLIDAIKSSLSQVRSEAESA--AGNPSSFALITTA 60
           MCSLQRRGN+FLLTLTGPGEHRLN  L+DAI+SS+ QVRSEAESA  AGN +SFALITTA
Sbjct: 1   MCSLQRRGNIFLLTLTGPGEHRLNLNLLDAIQSSVRQVRSEAESAVVAGNSASFALITTA 60

Query: 61  EGKFFSNGYDIDWAASSLDREYLMDDKLKSVASDLITLPMPTIAAVSGHASAAGFVLAMS 120
           EGKFFSNGYDIDWA SS+D+  LMDDKLKSV SDLITLPMPTIAAV+GHASAAGF+ AMS
Sbjct: 61  EGKFFSNGYDIDWAKSSMDKMILMDDKLKSVVSDLITLPMPTIAAVTGHASAAGFLFAMS 120

Query: 121 HDYILMRRDRGFLYMSELDIRRVIPSWFMALVKNKIGAPVARRDVVLKAPKLTADKALEM 180
           HDYILMRRDRGFLYMSELDIRRVIPSWF  LVK+KIG    RRDVVLKAPKLTAD+ALEM
Sbjct: 121 HDYILMRRDRGFLYMSELDIRRVIPSWFTVLVKSKIGTASVRRDVVLKAPKLTADRALEM 180

Query: 181 GIIDSAHDSAAETVAAAVRLGEELVARNWDGPIYARNRVELLSEVLNVIGTRCIVAKL 237
           GII+SAHD AAETV AAV+L EELVAR WDG IYA NR+ELLSEVLNVIG RC VAKL
Sbjct: 181 GIIESAHDGAAETVTAAVKLAEELVARKWDGHIYAGNRMELLSEVLNVIGARCSVAKL 238

[Close]

BLAST of Moc04g34600 vs. ExPASy TrEMBL
Match: A0A6J1JZ97 (enoyl-CoA delta isomerase 1, peroxisomal OS=Cucurbita maxima OX=3661 GN=LOC111489217 PE=4 SV=1)

HSP 1 Score: 378.6 bits (971), Expect = 1.9e-101
Identity = 198/238 (83.19%), Postives = 214/238 (89.92%), Query Frame = 0


Query: 1   MCSLQRRGNVFLLTLTGPGEHRLNPTLIDAIKSSLSQVRSEAESA--AGNPSSFALITTA 60
           MCSLQRRGN+FLLTLTG GEHRLN  L+DAI+SS+ QVRSEAESA  AG  +SFALITTA
Sbjct: 1   MCSLQRRGNIFLLTLTGSGEHRLNLNLLDAIQSSVRQVRSEAESAVVAGKSASFALITTA 60

Query: 61  EGKFFSNGYDIDWAASSLDREYLMDDKLKSVASDLITLPMPTIAAVSGHASAAGFVLAMS 120
           EGKFFSNGYDIDWA +S+D+  LMDDKLKSV SDLITLPMPTIAAV+GHASAAGF+LAMS
Sbjct: 61  EGKFFSNGYDIDWAKTSMDKMILMDDKLKSVVSDLITLPMPTIAAVTGHASAAGFLLAMS 120

Query: 121 HDYILMRRDRGFLYMSELDIRRVIPSWFMALVKNKIGAPVARRDVVLKAPKLTADKALEM 180
           HDYILMRRDRGFLYMSELDIRRVIPSWF  LVK+KIGA   RRDVVLKAPKLTAD+ALEM
Sbjct: 121 HDYILMRRDRGFLYMSELDIRRVIPSWFTVLVKSKIGAASVRRDVVLKAPKLTADEALEM 180

Query: 181 GIIDSAHDSAAETVAAAVRLGEELVARNWDGPIYARNRVELLSEVLNVIGTRCIVAKL 237
           GII+SAHD AAETVAAAV+L E+LVAR WDG IYA NR+ELLSEVLNVIG RC VAKL
Sbjct: 181 GIIESAHDGAAETVAAAVKLAEDLVARKWDGHIYAGNRMELLSEVLNVIGARCSVAKL 238

[Close]

BLAST of Moc04g34600 vs. ExPASy TrEMBL
Match: A0A1S3CKX3 (enoyl-CoA delta isomerase 1, peroxisomal OS=Cucumis melo OX=3656 GN=LOC103501637 PE=4 SV=1)

HSP 1 Score: 365.9 bits (938), Expect = 1.2e-97
Identity = 187/238 (78.57%), Postives = 206/238 (86.55%), Query Frame = 0


Query: 1   MCSLQRRGNVFLLTLTGPGEHRLNPTLIDAIKSSLSQVRSEAESAAGNPSS--FALITTA 60
           MCSLQR GN+F+LTLTGPGEHR NP L+D+I+SSL QVRS+ ESA    SS  F LITTA
Sbjct: 1   MCSLQRSGNIFILTLTGPGEHRFNPQLLDSIRSSLRQVRSDVESAVSTDSSSNFVLITTA 60

Query: 61  EGKFFSNGYDIDWAASSLDREYLMDDKLKSVASDLITLPMPTIAAVSGHASAAGFVLAMS 120
           EGKFFSNGYDIDW  +S+++  LMDDKLKSV SDLITLPMPTIAAVSGHASAAGF+ AMS
Sbjct: 61  EGKFFSNGYDIDWEKTSMNQMILMDDKLKSVVSDLITLPMPTIAAVSGHASAAGFIFAMS 120

Query: 121 HDYILMRRDRGFLYMSELDIRRVIPSWFMALVKNKIGAPVARRDVVLKAPKLTADKALEM 180
           HDYI+MRRDRGFLYMSELDIRRVIP WF  LVK++IG+   RR+VVLKAPKLTADKALE+
Sbjct: 121 HDYIVMRRDRGFLYMSELDIRRVIPQWFAVLVKSRIGSAAVRREVVLKAPKLTADKALEL 180

Query: 181 GIIDSAHDSAAETVAAAVRLGEELVARNWDGPIYARNRVELLSEVLNVIGTRCIVAKL 237
           GIIDSAHD A ETVAAAVRLGE+LVAR WDG  YA NR+ELLSEVLNVIG RC VAKL
Sbjct: 181 GIIDSAHDGAEETVAAAVRLGEDLVARKWDGHTYAGNRMELLSEVLNVIGARCSVAKL 238

[Close]

BLAST of Moc04g34600 vs. ExPASy TrEMBL
Match: A0A5A7VJZ2 (Enoyl-CoA delta isomerase 1, peroxisomal OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold98G003320 PE=4 SV=1)

HSP 1 Score: 362.8 bits (930), Expect = 1.1e-96
Identity = 186/238 (78.15%), Postives = 205/238 (86.13%), Query Frame = 0


Query: 1   MCSLQRRGNVFLLTLTGPGEHRLNPTLIDAIKSSLSQVRSEAESAAGNPSS--FALITTA 60
           MCSLQR GN+F+LTLTG GEHR NP L+D+I+SSL QVRS+ ESA    SS  F LITTA
Sbjct: 1   MCSLQRSGNIFILTLTGSGEHRFNPQLLDSIRSSLRQVRSDVESAVSTDSSSHFVLITTA 60

Query: 61  EGKFFSNGYDIDWAASSLDREYLMDDKLKSVASDLITLPMPTIAAVSGHASAAGFVLAMS 120
           EGKFFSNGYDIDW  +S+++  LMDDKLKSV SDLITLPMPTIAAVSGHASAAGF+ AMS
Sbjct: 61  EGKFFSNGYDIDWERTSMNQMILMDDKLKSVVSDLITLPMPTIAAVSGHASAAGFIFAMS 120

Query: 121 HDYILMRRDRGFLYMSELDIRRVIPSWFMALVKNKIGAPVARRDVVLKAPKLTADKALEM 180
           HDYI+MRRDRGFLYMSELDIRRVIP WF  LVK++IG+   RR+VVLKAPKLTADKALE+
Sbjct: 121 HDYIVMRRDRGFLYMSELDIRRVIPQWFAVLVKSRIGSAAVRREVVLKAPKLTADKALEL 180

Query: 181 GIIDSAHDSAAETVAAAVRLGEELVARNWDGPIYARNRVELLSEVLNVIGTRCIVAKL 237
           GIIDSAHD A ETVAAAVRLGE+LVAR WDG  YA NR+ELLSEVLNVIG RC VAKL
Sbjct: 181 GIIDSAHDGAEETVAAAVRLGEDLVARKWDGHTYAGNRMELLSEVLNVIGARCSVAKL 238

[Close]

BLAST of Moc04g34600 vs. TAIR 10
Match: AT1G65520.1 (delta(3), delta(2)-enoyl CoA isomerase 1 )

HSP 1 Score: 274.6 bits (701), Expect = 7.3e-74
Identity = 136/224 (60.71%), Postives = 179/224 (79.91%), Query Frame = 0


Query: 1   MCSLQRRGNVFLLTLTGPGEHRLNPTLIDAIKSSLSQVRSEAESAAGNPSSFALITTAEG 60
           MCSL++R  +F+L LTG GEHRLNPTL+D+++S+++Q+RS+        S   LITT++G
Sbjct: 1   MCSLEKRDRLFILKLTGDGEHRLNPTLLDSLRSTINQIRSDPSF-----SQSVLITTSDG 60

Query: 61  KFFSNGYDIDWAASSLDREYLMDDKLKSVASDLITLPMPTIAAVSGHASAAGFVLAMSHD 120
           KFFSNGYD+  A S+     +MD KL+S+ +DLI+LPMPTIAAV+GHASAAG +LAMSHD
Sbjct: 61  KFFSNGYDLALAESNPSLSVVMDAKLRSLVADLISLPMPTIAAVTGHASAAGCILAMSHD 120

Query: 121 YILMRRDRGFLYMSELDIRRVIPSWFMALVKNKIGAPVARRDVVLKAPKLTADKALEMGI 180
           Y+LMRRDRGFLYMSELDI  ++P+WFMA+++ KIG+P ARRDV+L A K+TAD  ++MGI
Sbjct: 121 YVLMRRDRGFLYMSELDIELIVPAWFMAVIRGKIGSPAARRDVMLTAAKVTADVGVKMGI 180

Query: 181 IDSAHDSAAETVAAAVRLGEELVARNWDGPIYARNRVELLSEVL 225
           +DSA+ SAAETV AA++LGEE+V R  DG +Y + R  LL EVL
Sbjct: 181 VDSAYGSAAETVEAAIKLGEEIVQRGGDGHVYGKMRESLLREVL 219

[Close]

BLAST of Moc04g34600 vs. TAIR 10
Match: AT4G14430.1 (indole-3-butyric acid response 10 )

HSP 1 Score: 215.3 bits (547), Expect = 5.2e-56
Identity = 113/235 (48.09%), Postives = 159/235 (67.66%), Query Frame = 0


Query: 1   MCSLQRRGNVFLLTLTGPGEHRLNPTLIDAIKSSLSQVRSEAESAAGNPSSFALITTAEG 60
           MC+L++RG++FLLTLTG GEHR +P  I  I S L Q +S++   +       LITTA G
Sbjct: 1   MCTLEKRGDLFLLTLTGDGEHRFHPDTIATILSLLEQAKSQSTRGS------ILITTANG 60

Query: 61  KFFSNGYDIDWA------ASSLDREYLMDDKLKSVASDLITLPMPTIAAVSGHASAAGFV 120
           KFFSNG+D+ WA        + +R + M +  K V + L+ LPMPTIAA++GHA+AAG +
Sbjct: 61  KFFSNGFDLAWAQTAGSKTGAANRLHQMVESFKPVVAALLDLPMPTIAALNGHAAAAGLI 120

Query: 121 LAMSHDYILMRRDRGFLYMSELDIRRVIPSWFMALVKNKIGAPVARRDVVLKAPKLTADK 180
           LA+SHDY+ MR+DRG LYMSE+DI   +P +F ALV+ KIG   ARR+++L   K+  ++
Sbjct: 121 LALSHDYVFMRKDRGVLYMSEVDIGLSMPDYFSALVRAKIGTSAARRELLLSGKKIRGEE 180

Query: 181 ALEMGIIDS-AHDSAAETVAAAVRLGEELVARNWDGPIYARNRVELLSEVLNVIG 229
           A+ +GI+DS A+DS    V A+VRLGE+L A+ W G +YA  R  L  E+  ++G
Sbjct: 181 AVGLGIVDSAAYDSEEGVVVASVRLGEKLAAKKWSGEVYASIRKSLYPELCGILG 229

[Close]

BLAST of Moc04g34600 vs. TAIR 10
Match: AT4G14440.1 (3-hydroxyacyl-CoA dehydratase 1 )

HSP 1 Score: 209.1 bits (531), Expect = 3.7e-54
Identity = 109/232 (46.98%), Postives = 156/232 (67.24%), Query Frame = 0


Query: 1   MCSLQRRGNVFLLTLTGPGEHRLNPTLIDAIKSSLSQVRSEAESAAGNPSSFALITTAEG 60
           MC+L++RG++FLLTLTG  EHR +P  I ++ S L Q +S++   +       LITT  G
Sbjct: 1   MCTLEKRGDLFLLTLTGEDEHRFHPDTIASVLSLLEQAKSQSTKGS------VLITTGHG 60

Query: 61  KFFSNGYDIDWAAS----SLDREYLMDDKLKSVASDLITLPMPTIAAVSGHASAAGFVLA 120
           KFFSNG+D+ WA S    ++ R + M    K V + L+ LPMPTIAA++GHA+A+G + A
Sbjct: 61  KFFSNGFDLAWAQSAGHGAIKRMHQMVKSFKPVLAALLDLPMPTIAALNGHAAASGLMFA 120

Query: 121 MSHDYILMRRDRGFLYMSELDIRRVIPSWFMALVKNKIGAPVARRDVVLKAPKLTADKAL 180
           +SHDY+ MR+DRG LYMSE+DI   +P +F ALV  K+G+ +ARR+++L   KL  ++A+
Sbjct: 121 LSHDYVFMRKDRGVLYMSEVDIGLPVPDYFSALVVAKVGSGIARRELLLSGKKLKGEEAV 180

Query: 181 EMGIIDS-AHDSAAETVAAAVRLGEELVARNWDGPIYARNRVELLSEVLNVI 228
            +GI+DS AHDSA   V A V LGE L A+ W+G +YA  R  L  E+  ++
Sbjct: 181 ALGIVDSAAHDSAEGVVEATVSLGESLAAKKWNGEVYATIRKSLYPELCRMV 226

The following BLAST results are available for this feature:

BLAST of Moc04g34600 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022133536.1	3.2e-124	100.00	enoyl-CoA delta isomerase 1, peroxisomal [Momordica charantia]	[more]
KAG6578713.1	1.2e-102	84.03	Enoyl-CoA delta isomerase 1, peroxisomal, partial [Cucurbita argyrosperma subsp....	[more]
XP_023550952.1	2.0e-102	83.19	enoyl-CoA delta isomerase 1, peroxisomal [Cucurbita pepo subsp. pepo]	[more]
XP_022939279.1	3.5e-102	83.61	enoyl-CoA delta isomerase 1, peroxisomal [Cucurbita moschata]	[more]
XP_022993099.1	3.8e-101	83.19	enoyl-CoA delta isomerase 1, peroxisomal [Cucurbita maxima]	[more]

BLAST of Moc04g34600 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
O04469	1.0e-72	60.71	Enoyl-CoA delta isomerase 1, peroxisomal OS=Arabidopsis thaliana OX=3702 GN=ECI1...	[more]
O23299	7.4e-55	48.09	Enoyl-CoA delta isomerase 2, peroxisomal OS=Arabidopsis thaliana OX=3702 GN=ECI2...	[more]
O23300	5.3e-53	46.98	Enoyl-CoA delta isomerase 3 OS=Arabidopsis thaliana OX=3702 GN=ECI3 PE=1 SV=1	[more]
Q8Z9L5	5.7e-07	26.00	Carnitinyl-CoA dehydratase OS=Salmonella typhi OX=90370 GN=caiD PE=3 SV=3	[more]
B5F749	7.5e-07	26.00	Carnitinyl-CoA dehydratase OS=Salmonella agona (strain SL483) OX=454166 GN=caiD ...	[more]

BLAST of Moc04g34600 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1BVD7	1.6e-124	100.00	enoyl-CoA delta isomerase 1, peroxisomal OS=Momordica charantia OX=3673 GN=LOC11...	[more]
A0A6J1FGP4	1.7e-102	83.61	enoyl-CoA delta isomerase 1, peroxisomal OS=Cucurbita moschata OX=3662 GN=LOC111...	[more]
A0A6J1JZ97	1.9e-101	83.19	enoyl-CoA delta isomerase 1, peroxisomal OS=Cucurbita maxima OX=3661 GN=LOC11148...	[more]
A0A1S3CKX3	1.2e-97	78.57	enoyl-CoA delta isomerase 1, peroxisomal OS=Cucumis melo OX=3656 GN=LOC103501637...	[more]
A0A5A7VJZ2	1.1e-96	78.15	Enoyl-CoA delta isomerase 1, peroxisomal OS=Cucumis melo var. makuwa OX=1194695 ...	[more]

BLAST of Moc04g34600 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G65520.1	7.3e-74	60.71	delta(3), delta(2)-enoyl CoA isomerase 1	[more]
AT4G14430.1	5.2e-56	48.09	indole-3-butyric acid response 10	[more]
AT4G14440.1	3.7e-54	46.98	3-hydroxyacyl-CoA dehydratase 1	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (OHB3-1) v2
Date Performed: 2022-08-01

Position :

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x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	GENE3D	3.90.226.10		coord: 1..222 e-value: 2.1E-35 score: 124.0
None	No IPR available	PANTHER	PTHR11941:SF84	ENOYL-COA DELTA ISOMERASE 1, PEROXISOMAL	coord: 1..229
None	No IPR available	PANTHER	PTHR11941	ENOYL-COA HYDRATASE-RELATED	coord: 1..229
None	No IPR available	CDD	cd06558	crotonase-like	coord: 2..202 e-value: 2.84591E-30 score: 109.187
IPR001753	Enoyl-CoA hydratase/isomerase	PFAM	PF00378	ECH_1	coord: 8..204 e-value: 6.0E-21 score: 74.9
IPR029045	ClpP/crotonase-like domain superfamily	SUPERFAMILY	52096	ClpP/crotonase	coord: 4..209

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Moc04g34600.1	Moc04g34600.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006635	fatty acid beta-oxidation
molecular_function	GO:0004165	dodecenoyl-CoA delta-isomerase activity
molecular_function	GO:0004300	enoyl-CoA hydratase activity
molecular_function	GO:0003824	catalytic activity