Moc04g33400 (gene) Bitter gourd (OHB3-1) v2

Overview
NameMoc04g33400
Typegene
OrganismMomordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
DescriptionProtein of unknown function (DUF668)
Locationchr4: 25158884 .. 25160716 (+)
RNA-Seq ExpressionMoc04g33400
SyntenyMoc04g33400
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGTTTCAGAATCTTGGTTTCGCAGTCTGTGGAAGCCTCCAAGGAAGCGTGAGTCTAGCACGCAGAAGGTCGTAATTGGAGTATTGGCATTTGAAATTGCTACCTTGATGTCCAAGTTGGTTCACCTATGGCAGTCCTTGAGCGATAAGCAAGTCAGGAGGCTGAGAGAAGAGATAAACAGTTCATTGGGCATAAAGAAGCTTGTGTCAGATAACGACGAGCATATAGTTCGTTTGATATATGCAGAGATGACCGAGAGTTTAGCTCATGTGGCTAAATCTGTTGCCAGACTGGGAAAGAAATGCAGTGATCCTAGTTTAAAAAACTTCGAGCATATTTTTGATGATTTAATTCAAATTGGAGCCGATCCCTATGGTTGGATATACTCATGGAAGAAGATGGAAAATAAGGTGAAGAAGATGGAGACATTTATTTCTGTCAATGCAAATTTGTATCAAGAGATGGAGATGCTTGCAGATCTTGAGCAAACGTTGAGGAGAATGAAGGTTAATGAGGAATCGGATGTAATCAATCTAGTCGAGTTTCAAAAGAAGGTTGCATGGAAACAGCAGGAGGTGAAAAATTTGCGAGAGATGTCACTTTGGAAAAGGACCTATGATTACTCAATTCTTCTGCTAGCTAGATCCCTAGCCACAATATTCAGTAGGATCAAACATGTATTTGGAATTGAGCAGAGTGAAGATAATGGTGAAATGAATGATTCACGAGATATGAATTCTGACTATATTGCACGTAGTCAATCAGTTTCTTCATTAATGCAATCCATGGTTCATCCTTCAGAGCACAACGGTCTTACTAAATTTGCTTCGGGTCCCCTGAAGAGGTTTACAACTAAATCAGGGCCAATTTCAAAAACTGCTAAAGCCAATAATTTCTATTCAGGTCCTCTTGGTAGCTCTATTACAAAATCAGGTCCTATTTCAGGTCCTGTTTCTGGAACCAACAGAAACTTCAACTCTTATTCAGGTCCTCTCACAAGTTCAACGATGAGATCAGGTCCAATTTCTGGAGTACACAACAAAACCAATCAGAAGAATTGGCTTGTCGGTGGCTACTCTTCCATATTTAATGGGAAGAAATCTCATCAGAAACCAAACAGACTAACTCAAGTTGGACCTTTTAAAGGATGCATGATATCTGGCACTAGTTCTCCAGTAGCGAATTGCCACATCAGTTCAAATGGGATTCATACAGAGCTTCTCAATGGAACCAAAGATGCTGGAAAGATTGTTGATCCTTGCAACAAAACCATTCCTTGCAAACAGTTGCTCGGTGCCAAGCGCCGATTATTGGATGCCCCACCCGAGACTCTAGGTGCTGCTGCTTTGGCACTGCACTATGCAAATGTCATTATTGTAATTGAGAAGCTTGCTGCATCTCCTCATTTGATTGGCCTGGATGCTAGAGATGATCTCTACAATATGCTGCCCGCCAAAGTTCGAGCTAGCCTTAGGGCATCCCTAAAGCCATATGCCAAGAGCTTGTCTTCATCTGCTTATGACACAGGTCTTGCAGGAGAGTGGAGTGAGGCAATAACTGGGATACTAGAATGGCTTGCTCCACTGGCTCACAACATGGTTAGATGGCAATCTGAACGTAGTTTTGAACAACAGAATTTTGTTTCCAGGACTAATATGCTTCTTGTACAAACCCTTTTCTTTGCAAATCAAGAAAAGACTGAAGCAATCATCACCGAACTTCTTGTTGGCCTGAATTACCTATGGAGGTTTGGTAGGGAACTCAATGCAAAAGCTCTCAATGAGTGTGCCAGCAGTAGAATACATGATGAATATTTGGATATCGTTGGGTAA

mRNA sequence

ATGGTTTCAGAATCTTGGTTTCGCAGTCTGTGGAAGCCTCCAAGGAAGCGTGAGTCTAGCACGCAGAAGGTCGTAATTGGAGTATTGGCATTTGAAATTGCTACCTTGATGTCCAAGTTGGTTCACCTATGGCAGTCCTTGAGCGATAAGCAAGTCAGGAGGCTGAGAGAAGAGATAAACAGTTCATTGGGCATAAAGAAGCTTGTGTCAGATAACGACGAGCATATAGTTCGTTTGATATATGCAGAGATGACCGAGAGTTTAGCTCATGTGGCTAAATCTGTTGCCAGACTGGGAAAGAAATGCAGTGATCCTAGTTTAAAAAACTTCGAGCATATTTTTGATGATTTAATTCAAATTGGAGCCGATCCCTATGGTTGGATATACTCATGGAAGAAGATGGAAAATAAGGTGAAGAAGATGGAGACATTTATTTCTGTCAATGCAAATTTGTATCAAGAGATGGAGATGCTTGCAGATCTTGAGCAAACGTTGAGGAGAATGAAGGTTAATGAGGAATCGGATGTAATCAATCTAGTCGAGTTTCAAAAGAAGGTTGCATGGAAACAGCAGGAGGTGAAAAATTTGCGAGAGATGTCACTTTGGAAAAGGACCTATGATTACTCAATTCTTCTGCTAGCTAGATCCCTAGCCACAATATTCAGTAGGATCAAACATGTATTTGGAATTGAGCAGAGTGAAGATAATGGTGAAATGAATGATTCACGAGATATGAATTCTGACTATATTGCACGTAGTCAATCAGTTTCTTCATTAATGCAATCCATGGTTCATCCTTCAGAGCACAACGGTCTTACTAAATTTGCTTCGGGTCCCCTGAAGAGGTTTACAACTAAATCAGGGCCAATTTCAAAAACTGCTAAAGCCAATAATTTCTATTCAGGTCCTCTTGGTAGCTCTATTACAAAATCAGGTCCTATTTCAGGTCCTGTTTCTGGAACCAACAGAAACTTCAACTCTTATTCAGGTCCTCTCACAAGTTCAACGATGAGATCAGGTCCAATTTCTGGAGTACACAACAAAACCAATCAGAAGAATTGGCTTGTCGGTGGCTACTCTTCCATATTTAATGGGAAGAAATCTCATCAGAAACCAAACAGACTAACTCAAGTTGGACCTTTTAAAGGATGCATGATATCTGGCACTAGTTCTCCAGTAGCGAATTGCCACATCAGTTCAAATGGGATTCATACAGAGCTTCTCAATGGAACCAAAGATGCTGGAAAGATTGTTGATCCTTGCAACAAAACCATTCCTTGCAAACAGTTGCTCGGTGCCAAGCGCCGATTATTGGATGCCCCACCCGAGACTCTAGGTGCTGCTGCTTTGGCACTGCACTATGCAAATGTCATTATTGTAATTGAGAAGCTTGCTGCATCTCCTCATTTGATTGGCCTGGATGCTAGAGATGATCTCTACAATATGCTGCCCGCCAAAGTTCGAGCTAGCCTTAGGGCATCCCTAAAGCCATATGCCAAGAGCTTGTCTTCATCTGCTTATGACACAGGTCTTGCAGGAGAGTGGAGTGAGGCAATAACTGGGATACTAGAATGGCTTGCTCCACTGGCTCACAACATGGTTAGATGGCAATCTGAACGTAGTTTTGAACAACAGAATTTTGTTTCCAGGACTAATATGCTTCTTGTACAAACCCTTTTCTTTGCAAATCAAGAAAAGACTGAAGCAATCATCACCGAACTTCTTGTTGGCCTGAATTACCTATGGAGGTTTGGTAGGGAACTCAATGCAAAAGCTCTCAATGAGTGTGCCAGCAGTAGAATACATGATGAATATTTGGATATCGTTGGGTAA

Coding sequence (CDS)

ATGGTTTCAGAATCTTGGTTTCGCAGTCTGTGGAAGCCTCCAAGGAAGCGTGAGTCTAGCACGCAGAAGGTCGTAATTGGAGTATTGGCATTTGAAATTGCTACCTTGATGTCCAAGTTGGTTCACCTATGGCAGTCCTTGAGCGATAAGCAAGTCAGGAGGCTGAGAGAAGAGATAAACAGTTCATTGGGCATAAAGAAGCTTGTGTCAGATAACGACGAGCATATAGTTCGTTTGATATATGCAGAGATGACCGAGAGTTTAGCTCATGTGGCTAAATCTGTTGCCAGACTGGGAAAGAAATGCAGTGATCCTAGTTTAAAAAACTTCGAGCATATTTTTGATGATTTAATTCAAATTGGAGCCGATCCCTATGGTTGGATATACTCATGGAAGAAGATGGAAAATAAGGTGAAGAAGATGGAGACATTTATTTCTGTCAATGCAAATTTGTATCAAGAGATGGAGATGCTTGCAGATCTTGAGCAAACGTTGAGGAGAATGAAGGTTAATGAGGAATCGGATGTAATCAATCTAGTCGAGTTTCAAAAGAAGGTTGCATGGAAACAGCAGGAGGTGAAAAATTTGCGAGAGATGTCACTTTGGAAAAGGACCTATGATTACTCAATTCTTCTGCTAGCTAGATCCCTAGCCACAATATTCAGTAGGATCAAACATGTATTTGGAATTGAGCAGAGTGAAGATAATGGTGAAATGAATGATTCACGAGATATGAATTCTGACTATATTGCACGTAGTCAATCAGTTTCTTCATTAATGCAATCCATGGTTCATCCTTCAGAGCACAACGGTCTTACTAAATTTGCTTCGGGTCCCCTGAAGAGGTTTACAACTAAATCAGGGCCAATTTCAAAAACTGCTAAAGCCAATAATTTCTATTCAGGTCCTCTTGGTAGCTCTATTACAAAATCAGGTCCTATTTCAGGTCCTGTTTCTGGAACCAACAGAAACTTCAACTCTTATTCAGGTCCTCTCACAAGTTCAACGATGAGATCAGGTCCAATTTCTGGAGTACACAACAAAACCAATCAGAAGAATTGGCTTGTCGGTGGCTACTCTTCCATATTTAATGGGAAGAAATCTCATCAGAAACCAAACAGACTAACTCAAGTTGGACCTTTTAAAGGATGCATGATATCTGGCACTAGTTCTCCAGTAGCGAATTGCCACATCAGTTCAAATGGGATTCATACAGAGCTTCTCAATGGAACCAAAGATGCTGGAAAGATTGTTGATCCTTGCAACAAAACCATTCCTTGCAAACAGTTGCTCGGTGCCAAGCGCCGATTATTGGATGCCCCACCCGAGACTCTAGGTGCTGCTGCTTTGGCACTGCACTATGCAAATGTCATTATTGTAATTGAGAAGCTTGCTGCATCTCCTCATTTGATTGGCCTGGATGCTAGAGATGATCTCTACAATATGCTGCCCGCCAAAGTTCGAGCTAGCCTTAGGGCATCCCTAAAGCCATATGCCAAGAGCTTGTCTTCATCTGCTTATGACACAGGTCTTGCAGGAGAGTGGAGTGAGGCAATAACTGGGATACTAGAATGGCTTGCTCCACTGGCTCACAACATGGTTAGATGGCAATCTGAACGTAGTTTTGAACAACAGAATTTTGTTTCCAGGACTAATATGCTTCTTGTACAAACCCTTTTCTTTGCAAATCAAGAAAAGACTGAAGCAATCATCACCGAACTTCTTGTTGGCCTGAATTACCTATGGAGGTTTGGTAGGGAACTCAATGCAAAAGCTCTCAATGAGTGTGCCAGCAGTAGAATACATGATGAATATTTGGATATCGTTGGGTAA

Protein sequence

MVSESWFRSLWKPPRKRESSTQKVVIGVLAFEIATLMSKLVHLWQSLSDKQVRRLREEINSSLGIKKLVSDNDEHIVRLIYAEMTESLAHVAKSVARLGKKCSDPSLKNFEHIFDDLIQIGADPYGWIYSWKKMENKVKKMETFISVNANLYQEMEMLADLEQTLRRMKVNEESDVINLVEFQKKVAWKQQEVKNLREMSLWKRTYDYSILLLARSLATIFSRIKHVFGIEQSEDNGEMNDSRDMNSDYIARSQSVSSLMQSMVHPSEHNGLTKFASGPLKRFTTKSGPISKTAKANNFYSGPLGSSITKSGPISGPVSGTNRNFNSYSGPLTSSTMRSGPISGVHNKTNQKNWLVGGYSSIFNGKKSHQKPNRLTQVGPFKGCMISGTSSPVANCHISSNGIHTELLNGTKDAGKIVDPCNKTIPCKQLLGAKRRLLDAPPETLGAAALALHYANVIIVIEKLAASPHLIGLDARDDLYNMLPAKVRASLRASLKPYAKSLSSSAYDTGLAGEWSEAITGILEWLAPLAHNMVRWQSERSFEQQNFVSRTNMLLVQTLFFANQEKTEAIITELLVGLNYLWRFGRELNAKALNECASSRIHDEYLDIVG
Homology
BLAST of Moc04g33400 vs. NCBI nr
Match: XP_022133904.1 (uncharacterized protein LOC111006336 [Momordica charantia] >XP_022133905.1 uncharacterized protein LOC111006336 [Momordica charantia] >XP_022133906.1 uncharacterized protein LOC111006336 [Momordica charantia])

HSP 1 Score: 1201.0 bits (3106), Expect = 0.0e+00
Identity = 610/610 (100.00%), Postives = 610/610 (100.00%), Query Frame = 0

Query: 1   MVSESWFRSLWKPPRKRESSTQKVVIGVLAFEIATLMSKLVHLWQSLSDKQVRRLREEIN 60
           MVSESWFRSLWKPPRKRESSTQKVVIGVLAFEIATLMSKLVHLWQSLSDKQVRRLREEIN
Sbjct: 1   MVSESWFRSLWKPPRKRESSTQKVVIGVLAFEIATLMSKLVHLWQSLSDKQVRRLREEIN 60

Query: 61  SSLGIKKLVSDNDEHIVRLIYAEMTESLAHVAKSVARLGKKCSDPSLKNFEHIFDDLIQI 120
           SSLGIKKLVSDNDEHIVRLIYAEMTESLAHVAKSVARLGKKCSDPSLKNFEHIFDDLIQI
Sbjct: 61  SSLGIKKLVSDNDEHIVRLIYAEMTESLAHVAKSVARLGKKCSDPSLKNFEHIFDDLIQI 120

Query: 121 GADPYGWIYSWKKMENKVKKMETFISVNANLYQEMEMLADLEQTLRRMKVNEESDVINLV 180
           GADPYGWIYSWKKMENKVKKMETFISVNANLYQEMEMLADLEQTLRRMKVNEESDVINLV
Sbjct: 121 GADPYGWIYSWKKMENKVKKMETFISVNANLYQEMEMLADLEQTLRRMKVNEESDVINLV 180

Query: 181 EFQKKVAWKQQEVKNLREMSLWKRTYDYSILLLARSLATIFSRIKHVFGIEQSEDNGEMN 240
           EFQKKVAWKQQEVKNLREMSLWKRTYDYSILLLARSLATIFSRIKHVFGIEQSEDNGEMN
Sbjct: 181 EFQKKVAWKQQEVKNLREMSLWKRTYDYSILLLARSLATIFSRIKHVFGIEQSEDNGEMN 240

Query: 241 DSRDMNSDYIARSQSVSSLMQSMVHPSEHNGLTKFASGPLKRFTTKSGPISKTAKANNFY 300
           DSRDMNSDYIARSQSVSSLMQSMVHPSEHNGLTKFASGPLKRFTTKSGPISKTAKANNFY
Sbjct: 241 DSRDMNSDYIARSQSVSSLMQSMVHPSEHNGLTKFASGPLKRFTTKSGPISKTAKANNFY 300

Query: 301 SGPLGSSITKSGPISGPVSGTNRNFNSYSGPLTSSTMRSGPISGVHNKTNQKNWLVGGYS 360
           SGPLGSSITKSGPISGPVSGTNRNFNSYSGPLTSSTMRSGPISGVHNKTNQKNWLVGGYS
Sbjct: 301 SGPLGSSITKSGPISGPVSGTNRNFNSYSGPLTSSTMRSGPISGVHNKTNQKNWLVGGYS 360

Query: 361 SIFNGKKSHQKPNRLTQVGPFKGCMISGTSSPVANCHISSNGIHTELLNGTKDAGKIVDP 420
           SIFNGKKSHQKPNRLTQVGPFKGCMISGTSSPVANCHISSNGIHTELLNGTKDAGKIVDP
Sbjct: 361 SIFNGKKSHQKPNRLTQVGPFKGCMISGTSSPVANCHISSNGIHTELLNGTKDAGKIVDP 420

Query: 421 CNKTIPCKQLLGAKRRLLDAPPETLGAAALALHYANVIIVIEKLAASPHLIGLDARDDLY 480
           CNKTIPCKQLLGAKRRLLDAPPETLGAAALALHYANVIIVIEKLAASPHLIGLDARDDLY
Sbjct: 421 CNKTIPCKQLLGAKRRLLDAPPETLGAAALALHYANVIIVIEKLAASPHLIGLDARDDLY 480

Query: 481 NMLPAKVRASLRASLKPYAKSLSSSAYDTGLAGEWSEAITGILEWLAPLAHNMVRWQSER 540
           NMLPAKVRASLRASLKPYAKSLSSSAYDTGLAGEWSEAITGILEWLAPLAHNMVRWQSER
Sbjct: 481 NMLPAKVRASLRASLKPYAKSLSSSAYDTGLAGEWSEAITGILEWLAPLAHNMVRWQSER 540

Query: 541 SFEQQNFVSRTNMLLVQTLFFANQEKTEAIITELLVGLNYLWRFGRELNAKALNECASSR 600
           SFEQQNFVSRTNMLLVQTLFFANQEKTEAIITELLVGLNYLWRFGRELNAKALNECASSR
Sbjct: 541 SFEQQNFVSRTNMLLVQTLFFANQEKTEAIITELLVGLNYLWRFGRELNAKALNECASSR 600

Query: 601 IHDEYLDIVG 611
           IHDEYLDIVG
Sbjct: 601 IHDEYLDIVG 610

BLAST of Moc04g33400 vs. NCBI nr
Match: XP_038885046.1 (protein PSK SIMULATOR 1 [Benincasa hispida] >XP_038885047.1 protein PSK SIMULATOR 1 [Benincasa hispida])

HSP 1 Score: 1079.3 bits (2790), Expect = 0.0e+00
Identity = 550/610 (90.16%), Postives = 576/610 (94.43%), Query Frame = 0

Query: 1   MVSESWFRSLWKPPRKRESSTQKVVIGVLAFEIATLMSKLVHLWQSLSDKQVRRLREEIN 60
           MVSESWFRSLWKPPRKR+ STQKVVIGVLAFEIA+LMSKLVHLWQSLSDKQV RLREEIN
Sbjct: 1   MVSESWFRSLWKPPRKRD-STQKVVIGVLAFEIASLMSKLVHLWQSLSDKQVGRLREEIN 60

Query: 61  SSLGIKKLVSDNDEHIVRLIYAEMTESLAHVAKSVARLGKKCSDPSLKNFEHIFDDLIQI 120
           +SLG+KKLVSD+DE+IVRLI AEMTESLAHVAKSVARLGKKCSDPSLKNFEH+FD LIQI
Sbjct: 61  NSLGLKKLVSDDDEYIVRLICAEMTESLAHVAKSVARLGKKCSDPSLKNFEHVFDALIQI 120

Query: 121 GADPYGWIYSWKKMENKVKKMETFISVNANLYQEMEMLADLEQTLRRMKVNEESDVINLV 180
           GADPYGWIYSWKKME KVKKMETFISVNANLYQEMEMLADLEQTLRRMKVN++SDVINLV
Sbjct: 121 GADPYGWIYSWKKMEKKVKKMETFISVNANLYQEMEMLADLEQTLRRMKVNDDSDVINLV 180

Query: 181 EFQKKVAWKQQEVKNLREMSLWKRTYDYSILLLARSLATIFSRIKHVFGIEQSEDNGEMN 240
           EFQKKVAWKQQEVKNLREMSLWKRTYDY+IL LARSL TIFSRIKHVF IEQS D    N
Sbjct: 181 EFQKKVAWKQQEVKNLREMSLWKRTYDYTILHLARSLFTIFSRIKHVFEIEQSVDTDGSN 240

Query: 241 DSRDMNSDYIARSQSVSSLMQSMVHPSEHNGLTKFASGPLKRFTTKSGPISKTAKANNFY 300
           DSRDMNSDYIARSQSVSSLMQSMVHPSE NGLTKFASGPLKRFTTKSGPISKT K NNFY
Sbjct: 241 DSRDMNSDYIARSQSVSSLMQSMVHPSE-NGLTKFASGPLKRFTTKSGPISKTTKPNNFY 300

Query: 301 SGPLGSSITKSGPISGPVSGTNRNFNSYSGPLTSSTMRSGPISGVHNKTNQKNWLVGGYS 360
           SGPLG SITKSGPISGP SGTNRNFNSYSGPLTSS +RSGPISG+ NKT+QKNWLVGGYS
Sbjct: 301 SGPLGGSITKSGPISGPGSGTNRNFNSYSGPLTSSAIRSGPISGIDNKTHQKNWLVGGYS 360

Query: 361 SIFNGKKSHQKPNRLTQVGPFKGCMISGTSSPVANCHISSNGIHTELLNGTKDAGKIVDP 420
           S+FNGKKSHQKPNRLTQVGPFKGCMISG SS VANCHISSNG H++L++G KD G  VD 
Sbjct: 361 SLFNGKKSHQKPNRLTQVGPFKGCMISGPSSMVANCHISSNGYHSQLISGAKDTGMTVDH 420

Query: 421 CNKTIPCKQLLGAKRRLLDAPPETLGAAALALHYANVIIVIEKLAASPHLIGLDARDDLY 480
           C++  PCKQLL  KRRLLDAPPETLG AALALHYANVIIVIEKLAASPHLIGLDARDDLY
Sbjct: 421 CDRANPCKQLLSTKRRLLDAPPETLGGAALALHYANVIIVIEKLAASPHLIGLDARDDLY 480

Query: 481 NMLPAKVRASLRASLKPYAKSLSSSAYDTGLAGEWSEAITGILEWLAPLAHNMVRWQSER 540
           NMLPAKVRA+LRA+LKPYAKSL+SS YDTGLAGEW+EAITGILEWLAPLAHNMVRWQSER
Sbjct: 481 NMLPAKVRANLRAALKPYAKSLASSTYDTGLAGEWNEAITGILEWLAPLAHNMVRWQSER 540

Query: 541 SFEQQNFVSRTNMLLVQTLFFANQEKTEAIITELLVGLNYLWRFGRELNAKALNECASSR 600
           SFEQQNFVSRTNMLLVQTLFFANQEKTEA+ITELLVGLNYLW+FGRELNAKALNECASSR
Sbjct: 541 SFEQQNFVSRTNMLLVQTLFFANQEKTEAVITELLVGLNYLWKFGRELNAKALNECASSR 600

Query: 601 IHDEYLDIVG 611
           IHDEYLDIVG
Sbjct: 601 IHDEYLDIVG 608

BLAST of Moc04g33400 vs. NCBI nr
Match: XP_004138676.1 (uncharacterized protein LOC101204446 [Cucumis sativus] >XP_004138677.1 uncharacterized protein LOC101204446 [Cucumis sativus] >XP_031736823.1 uncharacterized protein LOC101204446 [Cucumis sativus] >KGN63044.1 hypothetical protein Csa_021925 [Cucumis sativus])

HSP 1 Score: 1072.4 bits (2772), Expect = 1.4e-309
Identity = 549/610 (90.00%), Postives = 572/610 (93.77%), Query Frame = 0

Query: 1   MVSESWFRSLWKPPRKRESSTQKVVIGVLAFEIATLMSKLVHLWQSLSDKQVRRLREEIN 60
           MVSESWFRSLWKPPRKRE STQKVVIGVLAFEIA+LMSKLVHLWQSLSDKQV RLREEIN
Sbjct: 1   MVSESWFRSLWKPPRKRE-STQKVVIGVLAFEIASLMSKLVHLWQSLSDKQVGRLREEIN 60

Query: 61  SSLGIKKLVSDNDEHIVRLIYAEMTESLAHVAKSVARLGKKCSDPSLKNFEHIFDDLIQI 120
           +SLGIKKLVSD+DE+IVRLI AEMTE+L HVAKSVARLGKKCSDPSLKNFEH+FD LIQI
Sbjct: 61  NSLGIKKLVSDDDEYIVRLICAEMTENLVHVAKSVARLGKKCSDPSLKNFEHVFDALIQI 120

Query: 121 GADPYGWIYSWKKMENKVKKMETFISVNANLYQEMEMLADLEQTLRRMKVNEESDVINLV 180
           GADPYGWIYSWKKME KVKKMETFISVNANLYQEMEMLADLEQT  RMK NE+SDVINLV
Sbjct: 121 GADPYGWIYSWKKMEKKVKKMETFISVNANLYQEMEMLADLEQTFSRMKANEDSDVINLV 180

Query: 181 EFQKKVAWKQQEVKNLREMSLWKRTYDYSILLLARSLATIFSRIKHVFGIEQSEDNGEMN 240
           EF+KKVAWKQQEVKNLREMSLWKRTYDY+ILLLARSL TIFSRIK VF  EQS DN   +
Sbjct: 181 EFRKKVAWKQQEVKNLREMSLWKRTYDYTILLLARSLFTIFSRIKRVFENEQSVDNDGTD 240

Query: 241 DSRDMNSDYIARSQSVSSLMQSMVHPSEHNGLTKFASGPLKRFTTKSGPISKTAKANNFY 300
           DSRDM+SDYIARSQSVSSLMQSMVHPSE +GLTKFASGPLKRFTTKSGPISKTAK NNFY
Sbjct: 241 DSRDMSSDYIARSQSVSSLMQSMVHPSE-SGLTKFASGPLKRFTTKSGPISKTAKPNNFY 300

Query: 301 SGPLGSSITKSGPISGPVSGTNRNFNSYSGPLTSSTMRSGPISGVHNKTNQKNWLVGGYS 360
           SGPLGSSITKSGPISGPVSGTNRNFNSYSGPLTSS +RSGP SG+ NKTNQKNWLVGGYS
Sbjct: 301 SGPLGSSITKSGPISGPVSGTNRNFNSYSGPLTSSAIRSGPTSGIDNKTNQKNWLVGGYS 360

Query: 361 SIFNGKKSHQKPNRLTQVGPFKGCMISGTSSPVANCHISSNGIHTELLNGTKDAGKIVDP 420
           S+FNGKKSHQKPNRLTQVGPFKGCMISG SS VANCHISSNG H++L+NG KD G IV+ 
Sbjct: 361 SLFNGKKSHQKPNRLTQVGPFKGCMISGPSSMVANCHISSNGYHSQLINGAKDTGNIVEH 420

Query: 421 CNKTIPCKQLLGAKRRLLDAPPETLGAAALALHYANVIIVIEKLAASPHLIGLDARDDLY 480
           CN+  PCKQLL  K RLLDAPPETLG AALALHYANVIIVIEKLAASPHLIGLDARDDLY
Sbjct: 421 CNRASPCKQLLSTKCRLLDAPPETLGGAALALHYANVIIVIEKLAASPHLIGLDARDDLY 480

Query: 481 NMLPAKVRASLRASLKPYAKSLSSSAYDTGLAGEWSEAITGILEWLAPLAHNMVRWQSER 540
           NMLPAKVRASLRA+LKPYAKSL+SS YDTGLAGEW+EAI GILEWLAPLAHNMVRWQSER
Sbjct: 481 NMLPAKVRASLRAALKPYAKSLASSMYDTGLAGEWNEAIAGILEWLAPLAHNMVRWQSER 540

Query: 541 SFEQQNFVSRTNMLLVQTLFFANQEKTEAIITELLVGLNYLWRFGRELNAKALNECASSR 600
           SFEQQNFVSRTNMLLVQTLFFANQEKTEAIITELLVGLNYLW FGRELNAKALNECASSR
Sbjct: 541 SFEQQNFVSRTNMLLVQTLFFANQEKTEAIITELLVGLNYLWNFGRELNAKALNECASSR 600

Query: 601 IHDEYLDIVG 611
           IHDEYLDIVG
Sbjct: 601 IHDEYLDIVG 608

BLAST of Moc04g33400 vs. NCBI nr
Match: XP_008456574.1 (PREDICTED: uncharacterized protein LOC103496487 [Cucumis melo] >XP_008456575.1 PREDICTED: uncharacterized protein LOC103496487 [Cucumis melo] >TYK11741.1 uncharacterized protein E5676_scaffold304G00580 [Cucumis melo var. makuwa])

HSP 1 Score: 1069.3 bits (2764), Expect = 1.2e-308
Identity = 548/610 (89.84%), Postives = 574/610 (94.10%), Query Frame = 0

Query: 1   MVSESWFRSLWKPPRKRESSTQKVVIGVLAFEIATLMSKLVHLWQSLSDKQVRRLREEIN 60
           MVSESWFRSLWKPPRKRE S QKVVIGVLAFEIA+LMSKLVHLWQSLSDKQV RLREEIN
Sbjct: 1   MVSESWFRSLWKPPRKRE-SMQKVVIGVLAFEIASLMSKLVHLWQSLSDKQVGRLREEIN 60

Query: 61  SSLGIKKLVSDNDEHIVRLIYAEMTESLAHVAKSVARLGKKCSDPSLKNFEHIFDDLIQI 120
           +SLGIKKLVSD+DE+IVRLI AEMTE+LAHVAKSVARLGKKCSDPSLKNFEH+FD LIQI
Sbjct: 61  NSLGIKKLVSDDDEYIVRLICAEMTENLAHVAKSVARLGKKCSDPSLKNFEHVFDALIQI 120

Query: 121 GADPYGWIYSWKKMENKVKKMETFISVNANLYQEMEMLADLEQTLRRMKVNEESDVINLV 180
           GADPYGWIYSWKKME KVKKMETFISVNANLYQEMEMLADLEQT  RMK NE+SDVINLV
Sbjct: 121 GADPYGWIYSWKKMEKKVKKMETFISVNANLYQEMEMLADLEQTFSRMKANEDSDVINLV 180

Query: 181 EFQKKVAWKQQEVKNLREMSLWKRTYDYSILLLARSLATIFSRIKHVFGIEQSEDNGEMN 240
           EF+KKVAWKQQEVKNLREMSLWKRTYDY+ILLLARSL TIFSRIK VF IEQS DN   +
Sbjct: 181 EFRKKVAWKQQEVKNLREMSLWKRTYDYTILLLARSLFTIFSRIKRVFEIEQSVDNDGTD 240

Query: 241 DSRDMNSDYIARSQSVSSLMQSMVHPSEHNGLTKFASGPLKRFTTKSGPISKTAKANNFY 300
           DSRDM+SDYIARSQSVSSLMQSMVHPSE +GLTKFASGPLKRFTTKSGPISKTA+ +NFY
Sbjct: 241 DSRDMSSDYIARSQSVSSLMQSMVHPSE-SGLTKFASGPLKRFTTKSGPISKTAR-SNFY 300

Query: 301 SGPLGSSITKSGPISGPVSGTNRNFNSYSGPLTSSTMRSGPISGVHNKTNQKNWLVGGYS 360
           SGPLG SITKSGPISGPVSGTNRNFNSYSGPLTSS +RSGP SG+ NKTNQKNWLVGGYS
Sbjct: 301 SGPLGGSITKSGPISGPVSGTNRNFNSYSGPLTSSAIRSGPTSGIDNKTNQKNWLVGGYS 360

Query: 361 SIFNGKKSHQKPNRLTQVGPFKGCMISGTSSPVANCHISSNGIHTELLNGTKDAGKIVDP 420
           S+FNGKKSHQKPNRLTQVGPFKGCMISG SS VANCHISSNG H++L+NG KD GKIV+ 
Sbjct: 361 SLFNGKKSHQKPNRLTQVGPFKGCMISGPSSMVANCHISSNGYHSQLINGAKDTGKIVEH 420

Query: 421 CNKTIPCKQLLGAKRRLLDAPPETLGAAALALHYANVIIVIEKLAASPHLIGLDARDDLY 480
           CN+  PCKQLL  K RLLDAPPETLGAAALALHYANVIIVIEKLAASPHLIGLDARDDLY
Sbjct: 421 CNRASPCKQLLSTKCRLLDAPPETLGAAALALHYANVIIVIEKLAASPHLIGLDARDDLY 480

Query: 481 NMLPAKVRASLRASLKPYAKSLSSSAYDTGLAGEWSEAITGILEWLAPLAHNMVRWQSER 540
           NMLPAKVRASLRA+LKPYAKSL+SS YDTGLAGEW+EAI GILEWLAPLAHNMVRWQSER
Sbjct: 481 NMLPAKVRASLRAALKPYAKSLASSVYDTGLAGEWNEAIAGILEWLAPLAHNMVRWQSER 540

Query: 541 SFEQQNFVSRTNMLLVQTLFFANQEKTEAIITELLVGLNYLWRFGRELNAKALNECASSR 600
           SFEQQNFVSRTNMLLVQTLFFANQEKTEAIITELLVGLNYLW FGRELNAKALNECASSR
Sbjct: 541 SFEQQNFVSRTNMLLVQTLFFANQEKTEAIITELLVGLNYLWNFGRELNAKALNECASSR 600

Query: 601 IHDEYLDIVG 611
           +HDEYLDIVG
Sbjct: 601 VHDEYLDIVG 607

BLAST of Moc04g33400 vs. NCBI nr
Match: XP_022990285.1 (uncharacterized protein LOC111487207 [Cucurbita maxima] >XP_022990286.1 uncharacterized protein LOC111487207 [Cucurbita maxima])

HSP 1 Score: 1048.5 bits (2710), Expect = 2.2e-302
Identity = 527/610 (86.39%), Postives = 567/610 (92.95%), Query Frame = 0

Query: 1   MVSESWFRSLWKPPRKRESSTQKVVIGVLAFEIATLMSKLVHLWQSLSDKQVRRLREEIN 60
           MVSESWFRSLWKP RKR+SSTQKVVIGVLAFEIA+LMS+LVHLW SLSDKQ+RRLRE+I 
Sbjct: 1   MVSESWFRSLWKPQRKRDSSTQKVVIGVLAFEIASLMSRLVHLWHSLSDKQIRRLREDIT 60

Query: 61  SSLGIKKLVSDNDEHIVRLIYAEMTESLAHVAKSVARLGKKCSDPSLKNFEHIFDDLIQI 120
           +SLGIKKLVSD+DE+IVRLI AE+TE+LAHVAKSVARLGKKCSDPSLK FEH+FD+L+QI
Sbjct: 61  NSLGIKKLVSDDDEYIVRLICAEITENLAHVAKSVARLGKKCSDPSLKVFEHVFDELLQI 120

Query: 121 GADPYGWIYSWKKMENKVKKMETFISVNANLYQEMEMLADLEQTLRRMKVNEESDVINLV 180
           GADPY WIYSWKKME KVKKMETFISVNANLYQEMEML +LEQT RRMKVN+ +  +NL 
Sbjct: 121 GADPYCWIYSWKKMEKKVKKMETFISVNANLYQEMEMLVELEQTFRRMKVNDGTCSVNLA 180

Query: 181 EFQKKVAWKQQEVKNLREMSLWKRTYDYSILLLARSLATIFSRIKHVFGIEQSEDNGEMN 240
           +F+KKV WKQQEVKNLREMSLW+RTYDY+ILLLARS+ TIFSRIKHVFGIEQS    E N
Sbjct: 181 DFRKKVEWKQQEVKNLREMSLWRRTYDYTILLLARSIFTIFSRIKHVFGIEQSAGIDETN 240

Query: 241 DSRDMNSDYIARSQSVSSLMQSMVHPSEHNGLTKFASGPLKRFTTKSGPISKTAKANNFY 300
           DSRDMNSDYIARSQSVSSLMQSMVHPSE NGLTKFASGPLKRFTTKSGPISKTAK NNFY
Sbjct: 241 DSRDMNSDYIARSQSVSSLMQSMVHPSE-NGLTKFASGPLKRFTTKSGPISKTAKPNNFY 300

Query: 301 SGPLGSSITKSGPISGPVSGTNRNFNSYSGPLTSSTMRSGPISGVHNKTNQKNWLVGGYS 360
           SGPLGSS+TKSGPI GPVSGTNRNFNSYSGPLTSST+RSGP+SG++NKTN KNW  GGYS
Sbjct: 301 SGPLGSSVTKSGPIFGPVSGTNRNFNSYSGPLTSSTVRSGPVSGINNKTNHKNWFAGGYS 360

Query: 361 SIFNGKKSHQKPNRLTQVGPFKGCMISGTSSPVANCHISSNGIHTELLNGTKDAGKIVDP 420
           S+FNGKKSHQKPNRLTQVGPFKGCM+SG SS V NCHISSNG H++L+NG KD GKIVDP
Sbjct: 361 SLFNGKKSHQKPNRLTQVGPFKGCMMSGPSSTVTNCHISSNGQHSQLINGAKDTGKIVDP 420

Query: 421 CNKTIPCKQLLGAKRRLLDAPPETLGAAALALHYANVIIVIEKLAASPHLIGLDARDDLY 480
           CNK  PC++LLG K +LLDAPPETLG AALALHYANVIIVIEKLAASPHLIGLDARDDLY
Sbjct: 421 CNKANPCRELLGTKCQLLDAPPETLGGAALALHYANVIIVIEKLAASPHLIGLDARDDLY 480

Query: 481 NMLPAKVRASLRASLKPYAKSLSSSAYDTGLAGEWSEAITGILEWLAPLAHNMVRWQSER 540
           NMLPAKVRASLRA+LKPYAK L+SS YDTGLA EW++AI GILEWLAPLAHNMVRWQSER
Sbjct: 481 NMLPAKVRASLRAALKPYAKILASSTYDTGLAVEWNDAIAGILEWLAPLAHNMVRWQSER 540

Query: 541 SFEQQNFVSRTNMLLVQTLFFANQEKTEAIITELLVGLNYLWRFGRELNAKALNECASSR 600
           SFEQQNFVSRTNMLLVQTLFFANQEKTE IITELLVGLNYLWRFGRE NAKALNECASSR
Sbjct: 541 SFEQQNFVSRTNMLLVQTLFFANQEKTEVIITELLVGLNYLWRFGREHNAKALNECASSR 600

Query: 601 IHDEYLDIVG 611
           IHDE+LDIVG
Sbjct: 601 IHDEHLDIVG 609

BLAST of Moc04g33400 vs. ExPASy Swiss-Prot
Match: P0DO24 (Protein PSK SIMULATOR 3 OS=Arabidopsis thaliana OX=3702 GN=PSI3 PE=1 SV=1)

HSP 1 Score: 72.0 bits (175), Expect = 2.6e-11
Identity = 53/209 (25.36%), Postives = 105/209 (50.24%), Query Frame = 0

Query: 26  IGVLAFEIATLMSKLVHLWQSLSDKQVRRLREEINSSLGIKKLVSDNDEHIVRLIYAEMT 85
           +G+LAFE+A  + K  +L +SLS + +  L+  I  S G++ LVS++ + ++RL+ A+  
Sbjct: 149 LGILAFEVANTIVKSSNLIESLSKRNIEHLKGTILYSEGVQNLVSNDFDELLRLVAADKR 208

Query: 86  ESLAHVAKSVARLGKKCSDPSLKNFEHIFDDLIQIGADPYGWIYSWKKMENKVKKMETFI 145
           + L   +  V R G +  D    N +  FD   +I  +        +     V ++   +
Sbjct: 209 QELQVFSGEVVRFGNRSKDFQWHNLQRYFD---RISKELTPQRQLKEDAVLVVDQLMVLV 268

Query: 146 SVNANLYQEMEMLADLEQTLRRMKVNEESDVIN-----LVEFQKKVAWKQQEVKNLREMS 205
              A LYQE+++L  LE+   + +  EE+   +     L   + ++  +++ VK+L++ S
Sbjct: 269 QYTAELYQELQVLYRLEKDYEQKRREEENSANSSKGDGLAILKTELKAQRKVVKSLKKKS 328

Query: 206 LWKRTYDYSILLLARSLATIFSRIKHVFG 230
           LW R ++  +  L   +  +   I ++FG
Sbjct: 329 LWSRGFEEVMEKLVDIVHFLLLEIHNIFG 354


HSP 2 Score: 70.1 bits (170), Expect = 9.9e-11
Identity = 49/148 (33.11%), Postives = 78/148 (52.70%), Query Frame = 0

Query: 445 LGAAALALHYANVIIVIEKLAASPHLIGLDARDDLYNMLPAKVRASLRASLKPY--AKSL 504
           LG A LALHYAN+I+ I+ L A    I  +ARD LY  LP  ++ +LR+ +K +   K L
Sbjct: 372 LGPAGLALHYANIIVQIDTLVARASSITSNARDSLYQSLPPGIKLALRSKIKSFNVDKEL 431

Query: 505 SSSAYDTGLAGEWSEAITGILEWLAPLAHNMVR------WQSERSFEQQNFVSRT---NM 564
           S +        +  + +   L WL P+A N  +      W  E +    +F S+    ++
Sbjct: 432 SVT--------QIKDEMERTLHWLVPVAGNTTKAHHGFGWVGEWANTGTDFTSKPSGGDI 491

Query: 565 LLVQTLFFANQEKTEAIITELLVGLNYL 582
           L ++TL+ A++EKTE  I   ++ L +L
Sbjct: 492 LRIETLYHASKEKTEIYILGQIIWLQHL 511

BLAST of Moc04g33400 vs. ExPASy Swiss-Prot
Match: Q9SA91 (Protein PSK SIMULATOR 2 OS=Arabidopsis thaliana OX=3702 GN=PSI2 PE=2 SV=2)

HSP 1 Score: 67.8 bits (164), Expect = 4.9e-10
Identity = 59/224 (26.34%), Postives = 106/224 (47.32%), Query Frame = 0

Query: 26  IGVLAFEIATLMSKLVHLWQSLSDKQVRRLREEINSSLGIKKLVSDNDEHIVRLIYAEMT 85
           + +LAFE+A  ++K   L QSLS++ ++ +++++  S  +KKLVS +   +  L  ++  
Sbjct: 134 VTILAFEVANTIAKGAALLQSLSEENLKFMKKDMLHSEEVKKLVSTDTTELQILAASDKR 193

Query: 86  ESLAHVAKSVARLGKKCSDPSLKNFEHIFDDLIQIGADPYGWIYSWKKMENKVKKMETFI 145
           E L   +  V R G  C D    N +  F   +++  +           E +++++ T  
Sbjct: 194 EELDLFSGEVIRFGNMCKDLQWHNLDRYF---MKLDTENSQHKLLKDDAEARMQELVTLA 253

Query: 146 SVNANLYQEMEMLADLEQTLRRMKVNEESDVI-----NLVEFQKKVAWKQQEVKNLREMS 205
            + + LY E++ L   EQ  RR     ES  +      +V  Q ++  +++ VK+L++ S
Sbjct: 254 RITSELYHELQALDRFEQDYRRKLAEVESLNLPRRGEGIVILQNELKQQKKLVKSLQKKS 313

Query: 206 LWKRTYDYSILLLARSLATIFSRIKHVFGIEQSEDN-GEMNDSR 244
           LW +     I  L   ++ I   I  VFG     DN GE    R
Sbjct: 314 LWSQNLAEIIEKLVDVVSYIRQTIVEVFGNNGLRDNEGEQGRER 354

BLAST of Moc04g33400 vs. ExPASy Swiss-Prot
Match: Q9XID5 (Protein PSK SIMULATOR 1 OS=Arabidopsis thaliana OX=3702 GN=PSI1 PE=1 SV=2)

HSP 1 Score: 66.2 bits (160), Expect = 1.4e-09
Identity = 47/147 (31.97%), Postives = 75/147 (51.02%), Query Frame = 0

Query: 445 LGAAALALHYANVIIVIEKLAASPHLIGLDARDDLYNMLPAKVRASLRASLKPYAKSLSS 504
           LG+A LALHYAN+I  I+ L +    +    RD LY  LP  ++++LR+ ++ +   +  
Sbjct: 380 LGSAGLALHYANIITQIDTLVSRSSTMPASTRDALYQGLPPSIKSALRSRIQSF--QVKE 439

Query: 505 SAYDTGLAGEWSEAITGILEWLAPLAHNMVR----------WQSERSFEQQNFVSRTNML 564
                 +  E  +     L+WL P+A N  +          W S  S   Q    +T +L
Sbjct: 440 ELTVPQIKAEMEKT----LQWLVPVATNTTKAHHGFGWVGEWASSGSEANQRPAGQT-IL 499

Query: 565 LVQTLFFANQEKTEAIITELLVGLNYL 582
            + TL  A++EKTEA I +L+V L++L
Sbjct: 500 RIDTLHHADKEKTEAYILDLVVWLHHL 519

BLAST of Moc04g33400 vs. ExPASy TrEMBL
Match: A0A6J1BWJ5 (uncharacterized protein LOC111006336 OS=Momordica charantia OX=3673 GN=LOC111006336 PE=4 SV=1)

HSP 1 Score: 1201.0 bits (3106), Expect = 0.0e+00
Identity = 610/610 (100.00%), Postives = 610/610 (100.00%), Query Frame = 0

Query: 1   MVSESWFRSLWKPPRKRESSTQKVVIGVLAFEIATLMSKLVHLWQSLSDKQVRRLREEIN 60
           MVSESWFRSLWKPPRKRESSTQKVVIGVLAFEIATLMSKLVHLWQSLSDKQVRRLREEIN
Sbjct: 1   MVSESWFRSLWKPPRKRESSTQKVVIGVLAFEIATLMSKLVHLWQSLSDKQVRRLREEIN 60

Query: 61  SSLGIKKLVSDNDEHIVRLIYAEMTESLAHVAKSVARLGKKCSDPSLKNFEHIFDDLIQI 120
           SSLGIKKLVSDNDEHIVRLIYAEMTESLAHVAKSVARLGKKCSDPSLKNFEHIFDDLIQI
Sbjct: 61  SSLGIKKLVSDNDEHIVRLIYAEMTESLAHVAKSVARLGKKCSDPSLKNFEHIFDDLIQI 120

Query: 121 GADPYGWIYSWKKMENKVKKMETFISVNANLYQEMEMLADLEQTLRRMKVNEESDVINLV 180
           GADPYGWIYSWKKMENKVKKMETFISVNANLYQEMEMLADLEQTLRRMKVNEESDVINLV
Sbjct: 121 GADPYGWIYSWKKMENKVKKMETFISVNANLYQEMEMLADLEQTLRRMKVNEESDVINLV 180

Query: 181 EFQKKVAWKQQEVKNLREMSLWKRTYDYSILLLARSLATIFSRIKHVFGIEQSEDNGEMN 240
           EFQKKVAWKQQEVKNLREMSLWKRTYDYSILLLARSLATIFSRIKHVFGIEQSEDNGEMN
Sbjct: 181 EFQKKVAWKQQEVKNLREMSLWKRTYDYSILLLARSLATIFSRIKHVFGIEQSEDNGEMN 240

Query: 241 DSRDMNSDYIARSQSVSSLMQSMVHPSEHNGLTKFASGPLKRFTTKSGPISKTAKANNFY 300
           DSRDMNSDYIARSQSVSSLMQSMVHPSEHNGLTKFASGPLKRFTTKSGPISKTAKANNFY
Sbjct: 241 DSRDMNSDYIARSQSVSSLMQSMVHPSEHNGLTKFASGPLKRFTTKSGPISKTAKANNFY 300

Query: 301 SGPLGSSITKSGPISGPVSGTNRNFNSYSGPLTSSTMRSGPISGVHNKTNQKNWLVGGYS 360
           SGPLGSSITKSGPISGPVSGTNRNFNSYSGPLTSSTMRSGPISGVHNKTNQKNWLVGGYS
Sbjct: 301 SGPLGSSITKSGPISGPVSGTNRNFNSYSGPLTSSTMRSGPISGVHNKTNQKNWLVGGYS 360

Query: 361 SIFNGKKSHQKPNRLTQVGPFKGCMISGTSSPVANCHISSNGIHTELLNGTKDAGKIVDP 420
           SIFNGKKSHQKPNRLTQVGPFKGCMISGTSSPVANCHISSNGIHTELLNGTKDAGKIVDP
Sbjct: 361 SIFNGKKSHQKPNRLTQVGPFKGCMISGTSSPVANCHISSNGIHTELLNGTKDAGKIVDP 420

Query: 421 CNKTIPCKQLLGAKRRLLDAPPETLGAAALALHYANVIIVIEKLAASPHLIGLDARDDLY 480
           CNKTIPCKQLLGAKRRLLDAPPETLGAAALALHYANVIIVIEKLAASPHLIGLDARDDLY
Sbjct: 421 CNKTIPCKQLLGAKRRLLDAPPETLGAAALALHYANVIIVIEKLAASPHLIGLDARDDLY 480

Query: 481 NMLPAKVRASLRASLKPYAKSLSSSAYDTGLAGEWSEAITGILEWLAPLAHNMVRWQSER 540
           NMLPAKVRASLRASLKPYAKSLSSSAYDTGLAGEWSEAITGILEWLAPLAHNMVRWQSER
Sbjct: 481 NMLPAKVRASLRASLKPYAKSLSSSAYDTGLAGEWSEAITGILEWLAPLAHNMVRWQSER 540

Query: 541 SFEQQNFVSRTNMLLVQTLFFANQEKTEAIITELLVGLNYLWRFGRELNAKALNECASSR 600
           SFEQQNFVSRTNMLLVQTLFFANQEKTEAIITELLVGLNYLWRFGRELNAKALNECASSR
Sbjct: 541 SFEQQNFVSRTNMLLVQTLFFANQEKTEAIITELLVGLNYLWRFGRELNAKALNECASSR 600

Query: 601 IHDEYLDIVG 611
           IHDEYLDIVG
Sbjct: 601 IHDEYLDIVG 610

BLAST of Moc04g33400 vs. ExPASy TrEMBL
Match: A0A0A0LPX2 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G386120 PE=4 SV=1)

HSP 1 Score: 1072.4 bits (2772), Expect = 6.8e-310
Identity = 549/610 (90.00%), Postives = 572/610 (93.77%), Query Frame = 0

Query: 1   MVSESWFRSLWKPPRKRESSTQKVVIGVLAFEIATLMSKLVHLWQSLSDKQVRRLREEIN 60
           MVSESWFRSLWKPPRKRE STQKVVIGVLAFEIA+LMSKLVHLWQSLSDKQV RLREEIN
Sbjct: 1   MVSESWFRSLWKPPRKRE-STQKVVIGVLAFEIASLMSKLVHLWQSLSDKQVGRLREEIN 60

Query: 61  SSLGIKKLVSDNDEHIVRLIYAEMTESLAHVAKSVARLGKKCSDPSLKNFEHIFDDLIQI 120
           +SLGIKKLVSD+DE+IVRLI AEMTE+L HVAKSVARLGKKCSDPSLKNFEH+FD LIQI
Sbjct: 61  NSLGIKKLVSDDDEYIVRLICAEMTENLVHVAKSVARLGKKCSDPSLKNFEHVFDALIQI 120

Query: 121 GADPYGWIYSWKKMENKVKKMETFISVNANLYQEMEMLADLEQTLRRMKVNEESDVINLV 180
           GADPYGWIYSWKKME KVKKMETFISVNANLYQEMEMLADLEQT  RMK NE+SDVINLV
Sbjct: 121 GADPYGWIYSWKKMEKKVKKMETFISVNANLYQEMEMLADLEQTFSRMKANEDSDVINLV 180

Query: 181 EFQKKVAWKQQEVKNLREMSLWKRTYDYSILLLARSLATIFSRIKHVFGIEQSEDNGEMN 240
           EF+KKVAWKQQEVKNLREMSLWKRTYDY+ILLLARSL TIFSRIK VF  EQS DN   +
Sbjct: 181 EFRKKVAWKQQEVKNLREMSLWKRTYDYTILLLARSLFTIFSRIKRVFENEQSVDNDGTD 240

Query: 241 DSRDMNSDYIARSQSVSSLMQSMVHPSEHNGLTKFASGPLKRFTTKSGPISKTAKANNFY 300
           DSRDM+SDYIARSQSVSSLMQSMVHPSE +GLTKFASGPLKRFTTKSGPISKTAK NNFY
Sbjct: 241 DSRDMSSDYIARSQSVSSLMQSMVHPSE-SGLTKFASGPLKRFTTKSGPISKTAKPNNFY 300

Query: 301 SGPLGSSITKSGPISGPVSGTNRNFNSYSGPLTSSTMRSGPISGVHNKTNQKNWLVGGYS 360
           SGPLGSSITKSGPISGPVSGTNRNFNSYSGPLTSS +RSGP SG+ NKTNQKNWLVGGYS
Sbjct: 301 SGPLGSSITKSGPISGPVSGTNRNFNSYSGPLTSSAIRSGPTSGIDNKTNQKNWLVGGYS 360

Query: 361 SIFNGKKSHQKPNRLTQVGPFKGCMISGTSSPVANCHISSNGIHTELLNGTKDAGKIVDP 420
           S+FNGKKSHQKPNRLTQVGPFKGCMISG SS VANCHISSNG H++L+NG KD G IV+ 
Sbjct: 361 SLFNGKKSHQKPNRLTQVGPFKGCMISGPSSMVANCHISSNGYHSQLINGAKDTGNIVEH 420

Query: 421 CNKTIPCKQLLGAKRRLLDAPPETLGAAALALHYANVIIVIEKLAASPHLIGLDARDDLY 480
           CN+  PCKQLL  K RLLDAPPETLG AALALHYANVIIVIEKLAASPHLIGLDARDDLY
Sbjct: 421 CNRASPCKQLLSTKCRLLDAPPETLGGAALALHYANVIIVIEKLAASPHLIGLDARDDLY 480

Query: 481 NMLPAKVRASLRASLKPYAKSLSSSAYDTGLAGEWSEAITGILEWLAPLAHNMVRWQSER 540
           NMLPAKVRASLRA+LKPYAKSL+SS YDTGLAGEW+EAI GILEWLAPLAHNMVRWQSER
Sbjct: 481 NMLPAKVRASLRAALKPYAKSLASSMYDTGLAGEWNEAIAGILEWLAPLAHNMVRWQSER 540

Query: 541 SFEQQNFVSRTNMLLVQTLFFANQEKTEAIITELLVGLNYLWRFGRELNAKALNECASSR 600
           SFEQQNFVSRTNMLLVQTLFFANQEKTEAIITELLVGLNYLW FGRELNAKALNECASSR
Sbjct: 541 SFEQQNFVSRTNMLLVQTLFFANQEKTEAIITELLVGLNYLWNFGRELNAKALNECASSR 600

Query: 601 IHDEYLDIVG 611
           IHDEYLDIVG
Sbjct: 601 IHDEYLDIVG 608

BLAST of Moc04g33400 vs. ExPASy TrEMBL
Match: A0A1S3C3N3 (uncharacterized protein LOC103496487 OS=Cucumis melo OX=3656 GN=LOC103496487 PE=4 SV=1)

HSP 1 Score: 1069.3 bits (2764), Expect = 5.9e-309
Identity = 548/610 (89.84%), Postives = 574/610 (94.10%), Query Frame = 0

Query: 1   MVSESWFRSLWKPPRKRESSTQKVVIGVLAFEIATLMSKLVHLWQSLSDKQVRRLREEIN 60
           MVSESWFRSLWKPPRKRE S QKVVIGVLAFEIA+LMSKLVHLWQSLSDKQV RLREEIN
Sbjct: 1   MVSESWFRSLWKPPRKRE-SMQKVVIGVLAFEIASLMSKLVHLWQSLSDKQVGRLREEIN 60

Query: 61  SSLGIKKLVSDNDEHIVRLIYAEMTESLAHVAKSVARLGKKCSDPSLKNFEHIFDDLIQI 120
           +SLGIKKLVSD+DE+IVRLI AEMTE+LAHVAKSVARLGKKCSDPSLKNFEH+FD LIQI
Sbjct: 61  NSLGIKKLVSDDDEYIVRLICAEMTENLAHVAKSVARLGKKCSDPSLKNFEHVFDALIQI 120

Query: 121 GADPYGWIYSWKKMENKVKKMETFISVNANLYQEMEMLADLEQTLRRMKVNEESDVINLV 180
           GADPYGWIYSWKKME KVKKMETFISVNANLYQEMEMLADLEQT  RMK NE+SDVINLV
Sbjct: 121 GADPYGWIYSWKKMEKKVKKMETFISVNANLYQEMEMLADLEQTFSRMKANEDSDVINLV 180

Query: 181 EFQKKVAWKQQEVKNLREMSLWKRTYDYSILLLARSLATIFSRIKHVFGIEQSEDNGEMN 240
           EF+KKVAWKQQEVKNLREMSLWKRTYDY+ILLLARSL TIFSRIK VF IEQS DN   +
Sbjct: 181 EFRKKVAWKQQEVKNLREMSLWKRTYDYTILLLARSLFTIFSRIKRVFEIEQSVDNDGTD 240

Query: 241 DSRDMNSDYIARSQSVSSLMQSMVHPSEHNGLTKFASGPLKRFTTKSGPISKTAKANNFY 300
           DSRDM+SDYIARSQSVSSLMQSMVHPSE +GLTKFASGPLKRFTTKSGPISKTA+ +NFY
Sbjct: 241 DSRDMSSDYIARSQSVSSLMQSMVHPSE-SGLTKFASGPLKRFTTKSGPISKTAR-SNFY 300

Query: 301 SGPLGSSITKSGPISGPVSGTNRNFNSYSGPLTSSTMRSGPISGVHNKTNQKNWLVGGYS 360
           SGPLG SITKSGPISGPVSGTNRNFNSYSGPLTSS +RSGP SG+ NKTNQKNWLVGGYS
Sbjct: 301 SGPLGGSITKSGPISGPVSGTNRNFNSYSGPLTSSAIRSGPTSGIDNKTNQKNWLVGGYS 360

Query: 361 SIFNGKKSHQKPNRLTQVGPFKGCMISGTSSPVANCHISSNGIHTELLNGTKDAGKIVDP 420
           S+FNGKKSHQKPNRLTQVGPFKGCMISG SS VANCHISSNG H++L+NG KD GKIV+ 
Sbjct: 361 SLFNGKKSHQKPNRLTQVGPFKGCMISGPSSMVANCHISSNGYHSQLINGAKDTGKIVEH 420

Query: 421 CNKTIPCKQLLGAKRRLLDAPPETLGAAALALHYANVIIVIEKLAASPHLIGLDARDDLY 480
           CN+  PCKQLL  K RLLDAPPETLGAAALALHYANVIIVIEKLAASPHLIGLDARDDLY
Sbjct: 421 CNRASPCKQLLSTKCRLLDAPPETLGAAALALHYANVIIVIEKLAASPHLIGLDARDDLY 480

Query: 481 NMLPAKVRASLRASLKPYAKSLSSSAYDTGLAGEWSEAITGILEWLAPLAHNMVRWQSER 540
           NMLPAKVRASLRA+LKPYAKSL+SS YDTGLAGEW+EAI GILEWLAPLAHNMVRWQSER
Sbjct: 481 NMLPAKVRASLRAALKPYAKSLASSVYDTGLAGEWNEAIAGILEWLAPLAHNMVRWQSER 540

Query: 541 SFEQQNFVSRTNMLLVQTLFFANQEKTEAIITELLVGLNYLWRFGRELNAKALNECASSR 600
           SFEQQNFVSRTNMLLVQTLFFANQEKTEAIITELLVGLNYLW FGRELNAKALNECASSR
Sbjct: 541 SFEQQNFVSRTNMLLVQTLFFANQEKTEAIITELLVGLNYLWNFGRELNAKALNECASSR 600

Query: 601 IHDEYLDIVG 611
           +HDEYLDIVG
Sbjct: 601 VHDEYLDIVG 607

BLAST of Moc04g33400 vs. ExPASy TrEMBL
Match: A0A5D3CIT4 (Uncharacterized protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold304G00580 PE=4 SV=1)

HSP 1 Score: 1069.3 bits (2764), Expect = 5.9e-309
Identity = 548/610 (89.84%), Postives = 574/610 (94.10%), Query Frame = 0

Query: 1   MVSESWFRSLWKPPRKRESSTQKVVIGVLAFEIATLMSKLVHLWQSLSDKQVRRLREEIN 60
           MVSESWFRSLWKPPRKRE S QKVVIGVLAFEIA+LMSKLVHLWQSLSDKQV RLREEIN
Sbjct: 1   MVSESWFRSLWKPPRKRE-SMQKVVIGVLAFEIASLMSKLVHLWQSLSDKQVGRLREEIN 60

Query: 61  SSLGIKKLVSDNDEHIVRLIYAEMTESLAHVAKSVARLGKKCSDPSLKNFEHIFDDLIQI 120
           +SLGIKKLVSD+DE+IVRLI AEMTE+LAHVAKSVARLGKKCSDPSLKNFEH+FD LIQI
Sbjct: 61  NSLGIKKLVSDDDEYIVRLICAEMTENLAHVAKSVARLGKKCSDPSLKNFEHVFDALIQI 120

Query: 121 GADPYGWIYSWKKMENKVKKMETFISVNANLYQEMEMLADLEQTLRRMKVNEESDVINLV 180
           GADPYGWIYSWKKME KVKKMETFISVNANLYQEMEMLADLEQT  RMK NE+SDVINLV
Sbjct: 121 GADPYGWIYSWKKMEKKVKKMETFISVNANLYQEMEMLADLEQTFSRMKANEDSDVINLV 180

Query: 181 EFQKKVAWKQQEVKNLREMSLWKRTYDYSILLLARSLATIFSRIKHVFGIEQSEDNGEMN 240
           EF+KKVAWKQQEVKNLREMSLWKRTYDY+ILLLARSL TIFSRIK VF IEQS DN   +
Sbjct: 181 EFRKKVAWKQQEVKNLREMSLWKRTYDYTILLLARSLFTIFSRIKRVFEIEQSVDNDGTD 240

Query: 241 DSRDMNSDYIARSQSVSSLMQSMVHPSEHNGLTKFASGPLKRFTTKSGPISKTAKANNFY 300
           DSRDM+SDYIARSQSVSSLMQSMVHPSE +GLTKFASGPLKRFTTKSGPISKTA+ +NFY
Sbjct: 241 DSRDMSSDYIARSQSVSSLMQSMVHPSE-SGLTKFASGPLKRFTTKSGPISKTAR-SNFY 300

Query: 301 SGPLGSSITKSGPISGPVSGTNRNFNSYSGPLTSSTMRSGPISGVHNKTNQKNWLVGGYS 360
           SGPLG SITKSGPISGPVSGTNRNFNSYSGPLTSS +RSGP SG+ NKTNQKNWLVGGYS
Sbjct: 301 SGPLGGSITKSGPISGPVSGTNRNFNSYSGPLTSSAIRSGPTSGIDNKTNQKNWLVGGYS 360

Query: 361 SIFNGKKSHQKPNRLTQVGPFKGCMISGTSSPVANCHISSNGIHTELLNGTKDAGKIVDP 420
           S+FNGKKSHQKPNRLTQVGPFKGCMISG SS VANCHISSNG H++L+NG KD GKIV+ 
Sbjct: 361 SLFNGKKSHQKPNRLTQVGPFKGCMISGPSSMVANCHISSNGYHSQLINGAKDTGKIVEH 420

Query: 421 CNKTIPCKQLLGAKRRLLDAPPETLGAAALALHYANVIIVIEKLAASPHLIGLDARDDLY 480
           CN+  PCKQLL  K RLLDAPPETLGAAALALHYANVIIVIEKLAASPHLIGLDARDDLY
Sbjct: 421 CNRASPCKQLLSTKCRLLDAPPETLGAAALALHYANVIIVIEKLAASPHLIGLDARDDLY 480

Query: 481 NMLPAKVRASLRASLKPYAKSLSSSAYDTGLAGEWSEAITGILEWLAPLAHNMVRWQSER 540
           NMLPAKVRASLRA+LKPYAKSL+SS YDTGLAGEW+EAI GILEWLAPLAHNMVRWQSER
Sbjct: 481 NMLPAKVRASLRAALKPYAKSLASSVYDTGLAGEWNEAIAGILEWLAPLAHNMVRWQSER 540

Query: 541 SFEQQNFVSRTNMLLVQTLFFANQEKTEAIITELLVGLNYLWRFGRELNAKALNECASSR 600
           SFEQQNFVSRTNMLLVQTLFFANQEKTEAIITELLVGLNYLW FGRELNAKALNECASSR
Sbjct: 541 SFEQQNFVSRTNMLLVQTLFFANQEKTEAIITELLVGLNYLWNFGRELNAKALNECASSR 600

Query: 601 IHDEYLDIVG 611
           +HDEYLDIVG
Sbjct: 601 VHDEYLDIVG 607

BLAST of Moc04g33400 vs. ExPASy TrEMBL
Match: A0A6J1JMI9 (uncharacterized protein LOC111487207 OS=Cucurbita maxima OX=3661 GN=LOC111487207 PE=4 SV=1)

HSP 1 Score: 1048.5 bits (2710), Expect = 1.1e-302
Identity = 527/610 (86.39%), Postives = 567/610 (92.95%), Query Frame = 0

Query: 1   MVSESWFRSLWKPPRKRESSTQKVVIGVLAFEIATLMSKLVHLWQSLSDKQVRRLREEIN 60
           MVSESWFRSLWKP RKR+SSTQKVVIGVLAFEIA+LMS+LVHLW SLSDKQ+RRLRE+I 
Sbjct: 1   MVSESWFRSLWKPQRKRDSSTQKVVIGVLAFEIASLMSRLVHLWHSLSDKQIRRLREDIT 60

Query: 61  SSLGIKKLVSDNDEHIVRLIYAEMTESLAHVAKSVARLGKKCSDPSLKNFEHIFDDLIQI 120
           +SLGIKKLVSD+DE+IVRLI AE+TE+LAHVAKSVARLGKKCSDPSLK FEH+FD+L+QI
Sbjct: 61  NSLGIKKLVSDDDEYIVRLICAEITENLAHVAKSVARLGKKCSDPSLKVFEHVFDELLQI 120

Query: 121 GADPYGWIYSWKKMENKVKKMETFISVNANLYQEMEMLADLEQTLRRMKVNEESDVINLV 180
           GADPY WIYSWKKME KVKKMETFISVNANLYQEMEML +LEQT RRMKVN+ +  +NL 
Sbjct: 121 GADPYCWIYSWKKMEKKVKKMETFISVNANLYQEMEMLVELEQTFRRMKVNDGTCSVNLA 180

Query: 181 EFQKKVAWKQQEVKNLREMSLWKRTYDYSILLLARSLATIFSRIKHVFGIEQSEDNGEMN 240
           +F+KKV WKQQEVKNLREMSLW+RTYDY+ILLLARS+ TIFSRIKHVFGIEQS    E N
Sbjct: 181 DFRKKVEWKQQEVKNLREMSLWRRTYDYTILLLARSIFTIFSRIKHVFGIEQSAGIDETN 240

Query: 241 DSRDMNSDYIARSQSVSSLMQSMVHPSEHNGLTKFASGPLKRFTTKSGPISKTAKANNFY 300
           DSRDMNSDYIARSQSVSSLMQSMVHPSE NGLTKFASGPLKRFTTKSGPISKTAK NNFY
Sbjct: 241 DSRDMNSDYIARSQSVSSLMQSMVHPSE-NGLTKFASGPLKRFTTKSGPISKTAKPNNFY 300

Query: 301 SGPLGSSITKSGPISGPVSGTNRNFNSYSGPLTSSTMRSGPISGVHNKTNQKNWLVGGYS 360
           SGPLGSS+TKSGPI GPVSGTNRNFNSYSGPLTSST+RSGP+SG++NKTN KNW  GGYS
Sbjct: 301 SGPLGSSVTKSGPIFGPVSGTNRNFNSYSGPLTSSTVRSGPVSGINNKTNHKNWFAGGYS 360

Query: 361 SIFNGKKSHQKPNRLTQVGPFKGCMISGTSSPVANCHISSNGIHTELLNGTKDAGKIVDP 420
           S+FNGKKSHQKPNRLTQVGPFKGCM+SG SS V NCHISSNG H++L+NG KD GKIVDP
Sbjct: 361 SLFNGKKSHQKPNRLTQVGPFKGCMMSGPSSTVTNCHISSNGQHSQLINGAKDTGKIVDP 420

Query: 421 CNKTIPCKQLLGAKRRLLDAPPETLGAAALALHYANVIIVIEKLAASPHLIGLDARDDLY 480
           CNK  PC++LLG K +LLDAPPETLG AALALHYANVIIVIEKLAASPHLIGLDARDDLY
Sbjct: 421 CNKANPCRELLGTKCQLLDAPPETLGGAALALHYANVIIVIEKLAASPHLIGLDARDDLY 480

Query: 481 NMLPAKVRASLRASLKPYAKSLSSSAYDTGLAGEWSEAITGILEWLAPLAHNMVRWQSER 540
           NMLPAKVRASLRA+LKPYAK L+SS YDTGLA EW++AI GILEWLAPLAHNMVRWQSER
Sbjct: 481 NMLPAKVRASLRAALKPYAKILASSTYDTGLAVEWNDAIAGILEWLAPLAHNMVRWQSER 540

Query: 541 SFEQQNFVSRTNMLLVQTLFFANQEKTEAIITELLVGLNYLWRFGRELNAKALNECASSR 600
           SFEQQNFVSRTNMLLVQTLFFANQEKTE IITELLVGLNYLWRFGRE NAKALNECASSR
Sbjct: 541 SFEQQNFVSRTNMLLVQTLFFANQEKTEVIITELLVGLNYLWRFGREHNAKALNECASSR 600

Query: 601 IHDEYLDIVG 611
           IHDE+LDIVG
Sbjct: 601 IHDEHLDIVG 609

BLAST of Moc04g33400 vs. TAIR 10
Match: AT5G04550.1 (Protein of unknown function (DUF668) )

HSP 1 Score: 644.4 bits (1661), Expect = 9.0e-185
Identity = 351/614 (57.17%), Postives = 444/614 (72.31%), Query Frame = 0

Query: 1   MVSESWFRSLWKPPRKRESSTQKVVIGVLAFEIATLMSKLVHLWQSLSDKQVRRLREEIN 60
           MVSE+WFR+LW+ P+K +    K V+GVLAFE+A+L+SKLVHLWQSLSDK V RLR+EI 
Sbjct: 1   MVSETWFRNLWRFPKKHDGHKDKAVLGVLAFEVASLLSKLVHLWQSLSDKNVARLRDEIT 60

Query: 61  SSLGIKKLVSDNDEHIVRLIYAEMTESLAHVAKSVARLGKKCSDPSLKNFEHIFDDLIQI 120
            S GIKKLVS++D+ IVRLI  EM E++ +VAK+VARL +KC+DP LK FE+ F D+++ 
Sbjct: 61  HSTGIKKLVSEDDDFIVRLIRDEMMENVENVAKAVARLARKCNDPKLKCFENCFSDMMKT 120

Query: 121 GADPYGWIYSWKKMENKVKKMETFISVNANLYQEMEMLADLEQTLRRMKVNEESDVINLV 180
           GADPYGW + WKKM+ K KKME FIS NA+LYQE E+LADLEQT +RMK N ES   NL+
Sbjct: 121 GADPYGWQFGWKKMDKKAKKMERFISSNASLYQETEILADLEQTFKRMKSN-ESATDNLL 180

Query: 181 EFQKKVAWKQQEVKNLREMSLWKRTYDYSILLLARSLATIFSRIKHVFGIEQSEDNGEMN 240
           E+QKKV WK+ EVKNLR++SLW RTYDY+++LL RS+ TI SR KHVFGI       E +
Sbjct: 181 EYQKKVTWKRHEVKNLRDVSLWNRTYDYTVILLVRSVFTILSRTKHVFGISY---RVEAS 240

Query: 241 DSRDMNSDYIARSQSVSSLMQSMVHPSEHNGLTKFASGPLKRFT--TKSGPISKTAKANN 300
           D    +SD+I RS SVS+++  + H SE +GL +FASGPL RFT        +++ K ++
Sbjct: 241 DVSSADSDFIGRSHSVSTILTPVSHKSESSGLPRFASGPLGRFTGPASGSAATRSTKMSD 300

Query: 301 FYSGPLGSSITKSGPISGPVSGTNRNFNSYSGPLTSSTMRSGPISGVHNKTNQKNWLVGG 360
           F SG L +   KSGP+   V+  ++ F  YSG L   T +SGP+ G+  K N+K      
Sbjct: 301 FLSGSLSAESPKSGPL---VAEKHKRFKFYSGQLGKITSKSGPLIGM-GKNNKKMGQTPE 360

Query: 361 YSSIFNGKKSHQKPNRLTQVGPFKGCMISGTSSPVANCHISSNGIHTELLNGTKDAG--- 420
             SI + KK   K NRLTQVGPFKGCM+S         H     + T   NG +++    
Sbjct: 361 RPSISSVKK-QLKANRLTQVGPFKGCMVS---------HDGITPLSTRARNGARNSSAEH 420

Query: 421 KIVDPCNKTIPCKQL-LGAKRRLLDAPPETLGAAALALHYANVIIVIEKLAASPHLIGLD 480
            I++  + ++  + L L ++ +L DA P TLG A LALHYANVIIVIE+  ASPHLIG D
Sbjct: 421 HILEDNSNSVHVENLTLPSRPKLSDAAPNTLGTACLALHYANVIIVIERFVASPHLIGDD 480

Query: 481 ARDDLYNMLPAKVRASLRASLKPYAKSLSSS-AYDTGLAGEWSEAITGILEWLAPLAHNM 540
           ARDDLYNMLPA VR SLR  LKPY+K+LSSS  YD GLA EW++A+ GILEWL PLAHNM
Sbjct: 481 ARDDLYNMLPASVRTSLRERLKPYSKNLSSSTVYDPGLAREWTDAMAGILEWLGPLAHNM 540

Query: 541 VRWQSERSFEQQNFVSRTNMLLVQTLFFANQEKTEAIITELLVGLNYLWRFGRELNAKAL 600
           ++WQSERS+E Q+ VSRT+++L QTLFFANQ+KTEAIITELLVGLNY+WRFGRELNAKAL
Sbjct: 541 IKWQSERSYEHQSLVSRTHIVLAQTLFFANQQKTEAIITELLVGLNYVWRFGRELNAKAL 596

Query: 601 NECASSRIHDEYLD 608
            EC SS+  ++ LD
Sbjct: 601 QECTSSKTLEKCLD 596

BLAST of Moc04g33400 vs. TAIR 10
Match: AT3G23160.1 (Protein of unknown function (DUF668) )

HSP 1 Score: 320.9 bits (821), Expect = 2.3e-87
Identity = 210/586 (35.84%), Postives = 319/586 (54.44%), Query Frame = 0

Query: 20  STQKVVIGVLAFEIATLMSKLVHLWQSLSDKQVRRLREEINSSLGIKKLVSDNDEHIVRL 79
           S  K  IG+L+FE+A +MSK +HL +SLSD ++ +L+ E+  S G++KLVS ++ H++ L
Sbjct: 37  SLPKQTIGILSFEVANVMSKTIHLHRSLSDTEISKLKAEVFHSEGVRKLVSSDENHLLDL 96

Query: 80  IYAEMTESLAHVAKSVARLGKKCSDPSLKNFEHIFDDLIQIGADPYGWIYSWKKMENKVK 139
             +E  + L+ VA  V+RLGKKC++P+L+ FEH+++D++    D     +  K ME+ VK
Sbjct: 97  SVSEKLDDLSRVASVVSRLGKKCNEPALQGFEHVYEDIVNGAIDFRKLGFLVKDMESMVK 156

Query: 140 KMETFISVNANLYQEMEMLADLEQTLRRMKVNEESDVINLVEFQKKVAWKQQEVKNLREM 199
           KME F++   +LY EME++ +LEQ + +++ +++    ++  F++K+ W++Q+VK+LR+ 
Sbjct: 157 KMERFVNATCSLYCEMEVMNELEQAIVKLQRSQQHQE-SVKAFEQKLMWQRQDVKSLRDG 216

Query: 200 SLWKRTYDYSILLLARSLATIFSRIKHVF---GIEQSEDNGEMNDSRDMNSDYIARSQSV 259
           SLW +TYD  + +LAR++ TI+ RI+ VF   G+   +D     D     +     S+SV
Sbjct: 217 SLWNQTYDKVVEMLARTVCTIYGRIETVFGGLGLRGKKDVTLKRDRSKNEASKAVNSRSV 276

Query: 260 SSLMQSMVHPSEHNGLTKFASGPLKRFTTKSGPISKTAKANNFYSGPLGSSITKSGPISG 319
           +                                 S+ ++A+ F         T++G  + 
Sbjct: 277 AGFKD-----------------------------SRRSEADEF---------TRAGDFNF 336

Query: 320 PVSGTNRNFNSYSGPLTSSTMRSGPISGVHNKTNQKNWLVGGYSSIFNGKKSHQKPNRLT 379
           P  GTN                                                 P R+ 
Sbjct: 337 P-CGTN-------------------------------------------------PGRM- 396

Query: 380 QVGPFKGCMISGTSSPVANCHISSNGIHTELLNGTKDAGKIVDPCNKTIPCK-QLLGAKR 439
                              C   +  I  +  +  +D G+I  P +     +    G K 
Sbjct: 397 ----------------FMECLAMNRTIGDDDDDDDEDGGRITFPLSTARMIRSNKFGFKS 456

Query: 440 RLLD-APPETLGAAALALHYANVIIVIEKLAASPHLIGLDARDDLYNMLPAKVRASLRAS 499
           RL   A   T+G +AL+LHYANV+IV+EKL   PHLIG +ARDDLY MLP  ++ +L+AS
Sbjct: 457 RLTQHASASTIGGSALSLHYANVVIVVEKLLKYPHLIGEEARDDLYQMLPTSLKTTLKAS 512

Query: 500 LKPYAKSLSSSAYDTGLAGEWSEAITGILEWLAPLAHNMVRWQSERSFEQQN-FVSRTNM 559
           L+ Y K++  S YD  LA +W E I GIL WLAPLAHNM+RWQSER+FEQQN  V RTN+
Sbjct: 517 LRSYLKNI--SIYDAPLAHDWKETIDGILSWLAPLAHNMIRWQSERNFEQQNQIVKRTNV 512

Query: 560 LLVQTLFFANQEKTEAIITELLVGLNYLWRFGRELNAKALNECASS 600
           LL+QTL+FA++EKTEA I +LLVGLNY+  + ++ N  AL +CASS
Sbjct: 577 LLLQTLYFADREKTEAAICKLLVGLNYICHYEQQQN--ALLDCASS 512

BLAST of Moc04g33400 vs. TAIR 10
Match: AT5G51670.1 (Protein of unknown function (DUF668) )

HSP 1 Score: 289.7 bits (740), Expect = 5.6e-78
Identity = 197/590 (33.39%), Postives = 283/590 (47.97%), Query Frame = 0

Query: 20  STQKVVIGVLAFEIATLMSKLVHLWQSLSDKQVRRLREEINSSLGIKKLVSDNDEHIVRL 79
           ST    +GVL+FE+A +M+KL+HL  SL+D  +   R+   S  G+ K+V+ ++   + L
Sbjct: 31  STTTSSVGVLSFEVARVMTKLLHLTHSLTDSNLLTPRDHSLSLEGLTKIVNGDETFHLSL 90

Query: 80  IYAEMTESLAHVAKSVARLGKKCSDPSLKNFEHIFDDLIQIGADPYGWIYSWKKMENKVK 139
           + AE+ +SLAH A SV+RL  +C+  SL++F  +F +   +G DP+GW+ + K  E K K
Sbjct: 91  VCAELADSLAHAANSVSRLSNRCTTASLRSFHRLFHEFADMGRDPHGWVMNCKDTEAKNK 150

Query: 140 KMETFISVNANLYQEMEMLADLEQTLRRMKV------------NEESDVINLVEFQKKVA 199
           K+E ++SV   LY+EME +A LE +LR+  +              + DV+ +++ Q K+ 
Sbjct: 151 KIERYVSVTTALYREMEEMAILENSLRKQSLQIGIEFEEEEDYENKKDVMKVIDLQNKIE 210

Query: 200 WKQQEVKNLREMSLWKRTYDYSILLLARSLATIFSRIKHVFGIEQSEDNGEMNDSRDMNS 259
            ++Q VK L++ SLW +++D  +L+LARS+ T  +R+K VF                   
Sbjct: 211 RQKQHVKYLKDRSLWNKSFDTVVLILARSVFTALARLKSVF------------------- 270

Query: 260 DYIARSQSVSSLMQSMVHPSEHNGLTKFASGPLKRFTTKSGPISKTAKANNFYSGPLGSS 319
                                                        +A A  +    + SS
Sbjct: 271 --------------------------------------------SSAAATGYMVPTVVSS 330

Query: 320 ITKSGPISGPVSGTNRNFNSYSGPLTSSTMRSGPISGVHNKTNQKNWLVGGYSSIFNGKK 379
           + +S                                                        
Sbjct: 331 LPRS-------------------------------------------------------- 390

Query: 380 SHQKPNRLTQVGPFKGCMISGTSSPVANCHISSNGIHTELLNGTKDAGKIVDPCNKTIPC 439
                             +S +SS +   H S N                 +  +KT   
Sbjct: 391 ------------------LSSSSSSMNLVHPSPND----------------EERDKTTTS 450

Query: 440 KQLLGAKRRLLDAPPETLGAAALALHYANVIIVIEKLAASPHLIGLDARDDLYNMLPAKV 499
              L    RLL  P  TLG A +ALHYAN+I+V+EK+   P L+GLDARDDLY+MLPA V
Sbjct: 451 SAFLEESSRLLKPPETTLGGAGVALHYANLIVVMEKMIKQPQLVGLDARDDLYSMLPASV 463

Query: 500 RASLRASLKPYAKSLSSSAYDTGLAGEWSEAITGILEWLAPLAHNMVRWQSERSFEQQNF 559
           R+SLR+ L    K +  +A D GLA EW  A+  IL WL PLA NM+RWQSERSFEQQ+ 
Sbjct: 511 RSSLRSRL----KGVGFTATDGGLATEWKAALGRILRWLLPLAQNMIRWQSERSFEQQHM 463

Query: 560 VSRTN----MLLVQTLFFANQEKTEAIITELLVGLNYLWRFGRELNAKAL 594
            + TN    ++LVQTL FA++ KTEA ITELLVGLNY+WRF RE+ AKAL
Sbjct: 571 ATATNSQNRVMLVQTLVFADKVKTEAAITELLVGLNYIWRFEREMTAKAL 463

BLAST of Moc04g33400 vs. TAIR 10
Match: AT5G08660.1 (Protein of unknown function (DUF668) )

HSP 1 Score: 72.0 bits (175), Expect = 1.8e-12
Identity = 53/209 (25.36%), Postives = 105/209 (50.24%), Query Frame = 0

Query: 26  IGVLAFEIATLMSKLVHLWQSLSDKQVRRLREEINSSLGIKKLVSDNDEHIVRLIYAEMT 85
           +G+LAFE+A  + K  +L +SLS + +  L+  I  S G++ LVS++ + ++RL+ A+  
Sbjct: 149 LGILAFEVANTIVKSSNLIESLSKRNIEHLKGTILYSEGVQNLVSNDFDELLRLVAADKR 208

Query: 86  ESLAHVAKSVARLGKKCSDPSLKNFEHIFDDLIQIGADPYGWIYSWKKMENKVKKMETFI 145
           + L   +  V R G +  D    N +  FD   +I  +        +     V ++   +
Sbjct: 209 QELQVFSGEVVRFGNRSKDFQWHNLQRYFD---RISKELTPQRQLKEDAVLVVDQLMVLV 268

Query: 146 SVNANLYQEMEMLADLEQTLRRMKVNEESDVIN-----LVEFQKKVAWKQQEVKNLREMS 205
              A LYQE+++L  LE+   + +  EE+   +     L   + ++  +++ VK+L++ S
Sbjct: 269 QYTAELYQELQVLYRLEKDYEQKRREEENSANSSKGDGLAILKTELKAQRKVVKSLKKKS 328

Query: 206 LWKRTYDYSILLLARSLATIFSRIKHVFG 230
           LW R ++  +  L   +  +   I ++FG
Sbjct: 329 LWSRGFEEVMEKLVDIVHFLLLEIHNIFG 354


HSP 2 Score: 70.1 bits (170), Expect = 7.0e-12
Identity = 49/148 (33.11%), Postives = 78/148 (52.70%), Query Frame = 0

Query: 445 LGAAALALHYANVIIVIEKLAASPHLIGLDARDDLYNMLPAKVRASLRASLKPY--AKSL 504
           LG A LALHYAN+I+ I+ L A    I  +ARD LY  LP  ++ +LR+ +K +   K L
Sbjct: 372 LGPAGLALHYANIIVQIDTLVARASSITSNARDSLYQSLPPGIKLALRSKIKSFNVDKEL 431

Query: 505 SSSAYDTGLAGEWSEAITGILEWLAPLAHNMVR------WQSERSFEQQNFVSRT---NM 564
           S +        +  + +   L WL P+A N  +      W  E +    +F S+    ++
Sbjct: 432 SVT--------QIKDEMERTLHWLVPVAGNTTKAHHGFGWVGEWANTGTDFTSKPSGGDI 491

Query: 565 LLVQTLFFANQEKTEAIITELLVGLNYL 582
           L ++TL+ A++EKTE  I   ++ L +L
Sbjct: 492 LRIETLYHASKEKTEIYILGQIIWLQHL 511

BLAST of Moc04g33400 vs. TAIR 10
Match: AT1G30755.1 (Protein of unknown function (DUF668) )

HSP 1 Score: 67.8 bits (164), Expect = 3.5e-11
Identity = 59/224 (26.34%), Postives = 106/224 (47.32%), Query Frame = 0

Query: 26  IGVLAFEIATLMSKLVHLWQSLSDKQVRRLREEINSSLGIKKLVSDNDEHIVRLIYAEMT 85
           + +LAFE+A  ++K   L QSLS++ ++ +++++  S  +KKLVS +   +  L  ++  
Sbjct: 134 VTILAFEVANTIAKGAALLQSLSEENLKFMKKDMLHSEEVKKLVSTDTTELQILAASDKR 193

Query: 86  ESLAHVAKSVARLGKKCSDPSLKNFEHIFDDLIQIGADPYGWIYSWKKMENKVKKMETFI 145
           E L   +  V R G  C D    N +  F   +++  +           E +++++ T  
Sbjct: 194 EELDLFSGEVIRFGNMCKDLQWHNLDRYF---MKLDTENSQHKLLKDDAEARMQELVTLA 253

Query: 146 SVNANLYQEMEMLADLEQTLRRMKVNEESDVI-----NLVEFQKKVAWKQQEVKNLREMS 205
            + + LY E++ L   EQ  RR     ES  +      +V  Q ++  +++ VK+L++ S
Sbjct: 254 RITSELYHELQALDRFEQDYRRKLAEVESLNLPRRGEGIVILQNELKQQKKLVKSLQKKS 313

Query: 206 LWKRTYDYSILLLARSLATIFSRIKHVFGIEQSEDN-GEMNDSR 244
           LW +     I  L   ++ I   I  VFG     DN GE    R
Sbjct: 314 LWSQNLAEIIEKLVDVVSYIRQTIVEVFGNNGLRDNEGEQGRER 354

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022133904.10.0e+00100.00uncharacterized protein LOC111006336 [Momordica charantia] >XP_022133905.1 uncha... [more]
XP_038885046.10.0e+0090.16protein PSK SIMULATOR 1 [Benincasa hispida] >XP_038885047.1 protein PSK SIMULATO... [more]
XP_004138676.11.4e-30990.00uncharacterized protein LOC101204446 [Cucumis sativus] >XP_004138677.1 uncharact... [more]
XP_008456574.11.2e-30889.84PREDICTED: uncharacterized protein LOC103496487 [Cucumis melo] >XP_008456575.1 P... [more]
XP_022990285.12.2e-30286.39uncharacterized protein LOC111487207 [Cucurbita maxima] >XP_022990286.1 uncharac... [more]
Match NameE-valueIdentityDescription
P0DO242.6e-1125.36Protein PSK SIMULATOR 3 OS=Arabidopsis thaliana OX=3702 GN=PSI3 PE=1 SV=1[more]
Q9SA914.9e-1026.34Protein PSK SIMULATOR 2 OS=Arabidopsis thaliana OX=3702 GN=PSI2 PE=2 SV=2[more]
Q9XID51.4e-0931.97Protein PSK SIMULATOR 1 OS=Arabidopsis thaliana OX=3702 GN=PSI1 PE=1 SV=2[more]
Match NameE-valueIdentityDescription
A0A6J1BWJ50.0e+00100.00uncharacterized protein LOC111006336 OS=Momordica charantia OX=3673 GN=LOC111006... [more]
A0A0A0LPX26.8e-31090.00Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G386120 PE=4 SV=1[more]
A0A1S3C3N35.9e-30989.84uncharacterized protein LOC103496487 OS=Cucumis melo OX=3656 GN=LOC103496487 PE=... [more]
A0A5D3CIT45.9e-30989.84Uncharacterized protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold... [more]
A0A6J1JMI91.1e-30286.39uncharacterized protein LOC111487207 OS=Cucurbita maxima OX=3661 GN=LOC111487207... [more]
Match NameE-valueIdentityDescription
AT5G04550.19.0e-18557.17Protein of unknown function (DUF668) [more]
AT3G23160.12.3e-8735.84Protein of unknown function (DUF668) [more]
AT5G51670.15.6e-7833.39Protein of unknown function (DUF668) [more]
AT5G08660.11.8e-1225.36Protein of unknown function (DUF668) [more]
AT1G30755.13.5e-1126.34Protein of unknown function (DUF668) [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (OHB3-1) v2
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 179..199
NoneNo IPR availablePANTHERPTHR31371BNAC09G50660D PROTEINcoord: 1..607
NoneNo IPR availablePANTHERPTHR31371:SF22PLANT/T32M21-140 PROTEINcoord: 1..607
IPR021864Domain of unknown function DUF3475PFAMPF11961DUF3475coord: 28..84
e-value: 9.0E-24
score: 83.3
IPR007700Domain of unknown function DUF668PFAMPF05003DUF668coord: 444..535
e-value: 3.4E-32
score: 110.8

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Moc04g33400.1Moc04g33400.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0045927 positive regulation of growth