Moc04g33290 (gene) Bitter gourd (OHB3-1) v2

Overview
NameMoc04g33290
Typegene
OrganismMomordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
DescriptionnifU-like protein 4, mitochondrial
Locationchr4: 25064557 .. 25068176 (+)
RNA-Seq ExpressionMoc04g33290
SyntenyMoc04g33290
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGATTCTCGCGGAGCTGGGAAGATCCGACGCATTTCCACCGCTACAACTTCTTCTCTATTTGGCGGTGAAGACGCTGCTTCGTGTTCTACGACGTCTAGGTCTTGCTCCAATTCTTACTTGCGCTCCTGGAAATGGAACCAATTCGGAGGTAAGTGGATTTTGATGAATGGAAAAGTTGCATATGTAGTGTATTTTTTGTATCATCTGTCGGGCGATCACCGATCGTGACTTATTTAGTTTATATTGCATTCAGGACAGAGGAGAACAATGTTTATCCAAACTCAATCGACACCCAATCCTTCGTCTCTCATGTTTCATCCTGGAAAGCCTGTTATGGAAGTCGGGAGTGCGGATTTTCCAAATGCGCGTTCTGCCATGAACTCCCCACTTGCAAAAGCCCTTTATGGTATTGATGGTACGTATTCTGGAATTATATTTCTTTTTGGATTCAATACAACGTTTTTCAATTTTATTTTTCACGGTATATTATAATTTCCGAGTGTGAATTCCTTTAATTACCATTTAATTTCCTAAATGTGATGACTGGGGTTCTGCAAACATGTTGATGTTCGCATATGATTATTTTCTAAACAGAAGTTTTTTTGTTTTTTTTTTTGTTGAGAAAGTCGAAAAAGAAGTTAGTAAGTCATTAACGTTGTCTTTTATCTGATTACATTGATTGATTTATGGACTTTGAATAAGTGAAACCAGCATTAAGTGTAAACATGGGTTACTGGTGTACTATGCTCGTGATGAATATTTATCATGTAGAAGGGTGGAGGGCTGGAGGCGTAGAGGCAGGACATTTTTTCTGATGGCTGTTCGAAGAGTGTAATTAGTTTCCCTATAGGTTCGATAATTTTGCTTTTCATTTTATAGTGCAGGAGATGAAAGTTAAACTGAGTCCTGGAACCATGCGAATAAGAATCTTTTGATCTAAGTGTTTGGAACTTGAATGGCTGCAGACACATTGCTGATTCACATTGATTTGATTTTTTTAGTCTTTACCTCTTTCAATTGAAAACTGAGCTTCGGAATATCTTTGCTTGTGAGTATAGATAAGCTGCGCAGTGTAATGAATAAGTGGTACTAATCCAGATATAGATCATGGATGGTTACCTTACCAAAAATAACCATGCAATTTTATCAACTCTAATGATAATTTTGTCACTTCTTGTTATCCCATATGTAACGTTGTTGAATGTTAGTTTCAATTTTAATTATTACAAGCTGTTGATTTAGTGATGGTTACAGGTATACTTACTAGTTGTATTGATGGTTGGTGTGGGGCTTACTTGATTGCTTCTGTAGGTGTTGTACGAGTCTTCTTTGGATCAGATTTTGTCACTGTAACAAAATCGGAAGATGCTACTTGGGATTTCCTAAAGCCCGAAATTTTTGCCGCAATCATGGACTTCTATTCTTCTGGGCAACCACTGTTTCTAGACTCTAAAACTGCTGCTGCCATGGACACAGCCATCAATGAGGTATGTCTTTTAACTGCAAATTAATGCACATTCCTGTTAGATTTTTACTCGCTAAGAAACAAATTCATTTTTTAGAGCACATGTTGTTCTATTTGCTCTGAACTAAAAGTTGATGTTCTTACATATTTTTTTTTTCCCTGGGGATGTTTATGATAGTTTTATATTAGATTGTGTTTTGATTGAAAATTATGAACTTCTGTTTTGTTCTCTTTCCTCACTTTATATACCCTATGCTAATGTAATAAAAGGTAGAATGAGAGTTCCTTGGTGCAACACCGCTAATGAAATACATAGATTTTTAACTGTGTCTTAAATATTTATATAGAAGTATGAACATTTAATGTCATCATACTTAAGATTAAAATTACCATAATGTGGTGCCTTGCAGTTCTTTCACGGTGATGTTACTTCTACTGAAGACTTGTGCACCATATTCTTATTGAACTTGCAATATTATAATCTTATTCTTTTTTGGATAAAACTTTGGCTAATTACTTGTTTTCTGATCCCCACATACATCTTTTTTATGAAATTCAATTTTTCTTAGTCAAATTAGTAAGCTTTTGTTTTACGTTTTTTTCCCCTTCCTTTTCTTTCTTGCCTTCCTTTCCAGCTTTTCCATTAATACAAAAGGAGGATGCATGCTATTGCGCCTCTTGGAGACCTAGAGAATTGAAAAGGATTTCGCATAAATTTACTTCAATTGTATTCTCTGTTTTCAATCAATGAAATTCTCTTGTTAGTTGTCATTACAATTAATATGTTTCTTCAATGAAGTAGAGTTACTTATCTAAAGAATGATTATGTCAATATCTTCTGTCTGATGTCAAAATTTCGTTTGTAATATTTAATTTTGAGGTTTAACTTTACGTCTGTTGTTTACATGCTGCAAATTTTGAAACTTTCCTAAAGAGGTATATTTGTCATGTTGGAATTGGAAACATCATATATTTTATTTTATTTTTTTAGTAGTTTTTTTTTTTTGGATACAGCTCAATTCTTAGTAGATCCGTTCACTTGTGCCTACTTTTTAGGATGATTCTGAAATTGTTGCAATGATCAAAGAGCTGCTTGAGACTCGTATTCGACCTGCAGTTCAAGATGATGGTGGAGATATTGAATACAGGGGATATAACGAGTAGGTTTAGCTGGATCCAGTTGAAGGTGTTTCTTAAAGCATTTGAGTTGTATGCATGATTGCTTATATCTATTGAAGTTTACGCTTCTGTCTTCTTCAGAGAAACTGGTATAGTCACACTGAGAATGCAAGGAGCATGCAGTGGCTGCCCAAGCTCATCTGTCACTCTAAAATCTGGCATTGAAAATATGTTGATGCATTATGTACCTGAGGTAAGATTGATATGATCTTGGTATTTTTGTAAGCTTATTTTTAATTAGAAGCATATCCAATTTGTCACTTGCACAATTAGGTGTGCCGTATGTCCTTGCAAGCATTTTTGGCATAGTTGCTCGCACATGTGGCTTTTGTTTCCTGATATTTTATATTAGCAGCATCTTTTATATGATACCTGAGGTGTCCTACAAGCATAGTTATTCTTCGCATTTGGTTGCATACTGGAAGTGGAAGTATTGAGTTTGACTACACAACAGCTGAAGTGGAAGGATTTTTTTTTCTAAGATAAAGAGGAGTTGAAGCCTGAAGGACATTGAATCTTATTGTTATGACTACGAACTGTTGGTTTTCAACTTTTTTATCCGTCCATGGTAGTCAACTTCGTATAGGGCACCTGATTAACAAAGGCTTGTTATATTCAAGATAATATGTTCATATTTTACCAAATATAATCTTATGGAATGCAACATGCTCTAGTTGGATAACAAGAATGCCGAGGGGCATGTCTGATAAGAAAAAAGGTATTGCATCCTAGAGAGCCAATCTAGAGAAATCACTTTAAATGTGAAATATAATGAATGATGTTTCAGGGTTGCGCAGCCCAATTCAAATATATAGATTTTTTCCTCATCTTTTGTTCTTATGCTTCTCTGAATTATATATTATATATTTTAAATTTATTATCTATGATTTGATAGTATATTCTCAATTTATAGGTGAAGGGCGTGGAACAAGAATTTGATGTTGAGGAAGAGGATGCAATATCGACCAGCTAG

mRNA sequence

ATGGATTCTCGCGGAGCTGGGAAGATCCGACGCATTTCCACCGCTACAACTTCTTCTCTATTTGGCGGTGAAGACGCTGCTTCGTGTTCTACGACGTCTAGGTCTTGCTCCAATTCTTACTTGCGCTCCTGGAAATGGAACCAATTCGGAGGACAGAGGAGAACAATGTTTATCCAAACTCAATCGACACCCAATCCTTCGTCTCTCATGTTTCATCCTGGAAAGCCTGTTATGGAAGTCGGGAGTGCGGATTTTCCAAATGCGCGTTCTGCCATGAACTCCCCACTTGCAAAAGCCCTTTATGGTATTGATGGTGTTGTACGAGTCTTCTTTGGATCAGATTTTGTCACTGTAACAAAATCGGAAGATGCTACTTGGGATTTCCTAAAGCCCGAAATTTTTGCCGCAATCATGGACTTCTATTCTTCTGGGCAACCACTGTTTCTAGACTCTAAAACTGCTGCTGCCATGGACACAGCCATCAATGAGGATGATTCTGAAATTGTTGCAATGATCAAAGAGCTGCTTGAGACTCGTATTCGACCTGCAGTTCAAGATGATGGTGGAGATATTGAATACAGGGGATATAACGAAGAAACTGGTATAGTCACACTGAGAATGCAAGGAGCATGCAGTGGCTGCCCAAGCTCATCTGTCACTCTAAAATCTGGCATTGAAAATATGTTGATGCATTATGTACCTGAGGTGAAGGGCGTGGAACAAGAATTTGATGTTGAGGAAGAGGATGCAATATCGACCAGCTAG

Coding sequence (CDS)

ATGGATTCTCGCGGAGCTGGGAAGATCCGACGCATTTCCACCGCTACAACTTCTTCTCTATTTGGCGGTGAAGACGCTGCTTCGTGTTCTACGACGTCTAGGTCTTGCTCCAATTCTTACTTGCGCTCCTGGAAATGGAACCAATTCGGAGGACAGAGGAGAACAATGTTTATCCAAACTCAATCGACACCCAATCCTTCGTCTCTCATGTTTCATCCTGGAAAGCCTGTTATGGAAGTCGGGAGTGCGGATTTTCCAAATGCGCGTTCTGCCATGAACTCCCCACTTGCAAAAGCCCTTTATGGTATTGATGGTGTTGTACGAGTCTTCTTTGGATCAGATTTTGTCACTGTAACAAAATCGGAAGATGCTACTTGGGATTTCCTAAAGCCCGAAATTTTTGCCGCAATCATGGACTTCTATTCTTCTGGGCAACCACTGTTTCTAGACTCTAAAACTGCTGCTGCCATGGACACAGCCATCAATGAGGATGATTCTGAAATTGTTGCAATGATCAAAGAGCTGCTTGAGACTCGTATTCGACCTGCAGTTCAAGATGATGGTGGAGATATTGAATACAGGGGATATAACGAAGAAACTGGTATAGTCACACTGAGAATGCAAGGAGCATGCAGTGGCTGCCCAAGCTCATCTGTCACTCTAAAATCTGGCATTGAAAATATGTTGATGCATTATGTACCTGAGGTGAAGGGCGTGGAACAAGAATTTGATGTTGAGGAAGAGGATGCAATATCGACCAGCTAG

Protein sequence

MDSRGAGKIRRISTATTSSLFGGEDAASCSTTSRSCSNSYLRSWKWNQFGGQRRTMFIQTQSTPNPSSLMFHPGKPVMEVGSADFPNARSAMNSPLAKALYGIDGVVRVFFGSDFVTVTKSEDATWDFLKPEIFAAIMDFYSSGQPLFLDSKTAAAMDTAINEDDSEIVAMIKELLETRIRPAVQDDGGDIEYRGYNEETGIVTLRMQGACSGCPSSSVTLKSGIENMLMHYVPEVKGVEQEFDVEEEDAISTS
Homology
BLAST of Moc04g33290 vs. NCBI nr
Match: XP_022133710.1 (nifU-like protein 4, mitochondrial [Momordica charantia])

HSP 1 Score: 504.2 bits (1297), Expect = 6.5e-139
Identity = 254/254 (100.00%), Postives = 254/254 (100.00%), Query Frame = 0

Query: 1   MDSRGAGKIRRISTATTSSLFGGEDAASCSTTSRSCSNSYLRSWKWNQFGGQRRTMFIQT 60
           MDSRGAGKIRRISTATTSSLFGGEDAASCSTTSRSCSNSYLRSWKWNQFGGQRRTMFIQT
Sbjct: 17  MDSRGAGKIRRISTATTSSLFGGEDAASCSTTSRSCSNSYLRSWKWNQFGGQRRTMFIQT 76

Query: 61  QSTPNPSSLMFHPGKPVMEVGSADFPNARSAMNSPLAKALYGIDGVVRVFFGSDFVTVTK 120
           QSTPNPSSLMFHPGKPVMEVGSADFPNARSAMNSPLAKALYGIDGVVRVFFGSDFVTVTK
Sbjct: 77  QSTPNPSSLMFHPGKPVMEVGSADFPNARSAMNSPLAKALYGIDGVVRVFFGSDFVTVTK 136

Query: 121 SEDATWDFLKPEIFAAIMDFYSSGQPLFLDSKTAAAMDTAINEDDSEIVAMIKELLETRI 180
           SEDATWDFLKPEIFAAIMDFYSSGQPLFLDSKTAAAMDTAINEDDSEIVAMIKELLETRI
Sbjct: 137 SEDATWDFLKPEIFAAIMDFYSSGQPLFLDSKTAAAMDTAINEDDSEIVAMIKELLETRI 196

Query: 181 RPAVQDDGGDIEYRGYNEETGIVTLRMQGACSGCPSSSVTLKSGIENMLMHYVPEVKGVE 240
           RPAVQDDGGDIEYRGYNEETGIVTLRMQGACSGCPSSSVTLKSGIENMLMHYVPEVKGVE
Sbjct: 197 RPAVQDDGGDIEYRGYNEETGIVTLRMQGACSGCPSSSVTLKSGIENMLMHYVPEVKGVE 256

Query: 241 QEFDVEEEDAISTS 255
           QEFDVEEEDAISTS
Sbjct: 257 QEFDVEEEDAISTS 270

BLAST of Moc04g33290 vs. NCBI nr
Match: XP_038884119.1 (nifU-like protein 4, mitochondrial [Benincasa hispida])

HSP 1 Score: 454.9 bits (1169), Expect = 4.5e-124
Identity = 229/254 (90.16%), Postives = 238/254 (93.70%), Query Frame = 0

Query: 1   MDSRGAGKIRRISTATTSSLFGGEDAASCSTTSRSCSNSYLRSWKWNQFGGQRRTMFIQT 60
           M   G GK R  S ATTSS+FGGE+AASCS +SR+CSNSYLRSWK NQFGGQRRTMFIQT
Sbjct: 17  MAFHGGGKTRFFSAATTSSMFGGEEAASCSISSRNCSNSYLRSWKSNQFGGQRRTMFIQT 76

Query: 61  QSTPNPSSLMFHPGKPVMEVGSADFPNARSAMNSPLAKALYGIDGVVRVFFGSDFVTVTK 120
           QSTPNPSSLMF+PGKPVMEVGSADFPNARSAMNSPLAKALYG+DGVVRVFFGSDFVTVTK
Sbjct: 77  QSTPNPSSLMFYPGKPVMEVGSADFPNARSAMNSPLAKALYGVDGVVRVFFGSDFVTVTK 136

Query: 121 SEDATWDFLKPEIFAAIMDFYSSGQPLFLDSKTAAAMDTAINEDDSEIVAMIKELLETRI 180
           S DA+WDFLKPEIFAAIMDFYSSGQPLFLDSKTAAAMDTAI EDDSE VAMIKELLETRI
Sbjct: 137 SNDASWDFLKPEIFAAIMDFYSSGQPLFLDSKTAAAMDTAIKEDDSETVAMIKELLETRI 196

Query: 181 RPAVQDDGGDIEYRGYNEETGIVTLRMQGACSGCPSSSVTLKSGIENMLMHYVPEVKGVE 240
           RPAVQDDGGDIEYRGYNEETGIV LRMQGACSGCPSSSVTLKSGIENMLMHYVPEVKGVE
Sbjct: 197 RPAVQDDGGDIEYRGYNEETGIVKLRMQGACSGCPSSSVTLKSGIENMLMHYVPEVKGVE 256

Query: 241 QEFDVEEEDAISTS 255
           QEFD++EED  STS
Sbjct: 257 QEFDLDEEDVKSTS 270

BLAST of Moc04g33290 vs. NCBI nr
Match: XP_022960817.1 (nifU-like protein 4, mitochondrial [Cucurbita moschata])

HSP 1 Score: 448.7 bits (1153), Expect = 3.2e-122
Identity = 225/254 (88.58%), Postives = 241/254 (94.88%), Query Frame = 0

Query: 1   MDSRGAGKIRRISTATTSSLFGGEDAASCSTTSRSCSNSYLRSWKWNQFGGQRRTMFIQT 60
           ++SRG GKIR +S A TSSLF GE+AAS S +SR+ S+SYLRSWKWN+FGGQRRTMFI+T
Sbjct: 17  LNSRGVGKIRFVSAAATSSLFRGEEAASDSVSSRTFSDSYLRSWKWNRFGGQRRTMFIET 76

Query: 61  QSTPNPSSLMFHPGKPVMEVGSADFPNARSAMNSPLAKALYGIDGVVRVFFGSDFVTVTK 120
           QSTPNPSSLMF+PGKPVMEVGSADFPNARSAMNSPLA+ALYG+DGVVRVFFGSDFVTVTK
Sbjct: 77  QSTPNPSSLMFYPGKPVMEVGSADFPNARSAMNSPLARALYGVDGVVRVFFGSDFVTVTK 136

Query: 121 SEDATWDFLKPEIFAAIMDFYSSGQPLFLDSKTAAAMDTAINEDDSEIVAMIKELLETRI 180
           S DA+WDFLKPEIFAAIMDFYSSGQPLFLDS+TAAAMDTAINEDDSE VAMIKELLETRI
Sbjct: 137 SNDASWDFLKPEIFAAIMDFYSSGQPLFLDSQTAAAMDTAINEDDSETVAMIKELLETRI 196

Query: 181 RPAVQDDGGDIEYRGYNEETGIVTLRMQGACSGCPSSSVTLKSGIENMLMHYVPEVKGVE 240
           RPAVQDDGGDIEYRGY+EETGIV LRMQGACSGCPSSSVTLKSGIENMLMHYVPEVKGVE
Sbjct: 197 RPAVQDDGGDIEYRGYDEETGIVKLRMQGACSGCPSSSVTLKSGIENMLMHYVPEVKGVE 256

Query: 241 QEFDVEEEDAISTS 255
           QEFDVEE+D  STS
Sbjct: 257 QEFDVEEDDIKSTS 270

BLAST of Moc04g33290 vs. NCBI nr
Match: KAG6578816.1 (NifU-like protein 4, mitochondrial, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 447.2 bits (1149), Expect = 9.4e-122
Identity = 220/254 (86.61%), Postives = 238/254 (93.70%), Query Frame = 0

Query: 1   MDSRGAGKIRRISTATTSSLFGGEDAASCSTTSRSCSNSYLRSWKWNQFGGQRRTMFIQT 60
           + S G GKIR  S ATTSS+FGG++AASCS +SR+CS+  LRSW+W+QFGGQ+RTMFI+T
Sbjct: 17  LGSHGVGKIRFFSAATTSSIFGGKEAASCSVSSRTCSDPLLRSWRWSQFGGQKRTMFIET 76

Query: 61  QSTPNPSSLMFHPGKPVMEVGSADFPNARSAMNSPLAKALYGIDGVVRVFFGSDFVTVTK 120
           QSTPNPSSLMF+PGKPVMEVGSADFPNARSAMNSPLAKALYG+DGV+RVFFGSDFVTVTK
Sbjct: 77  QSTPNPSSLMFYPGKPVMEVGSADFPNARSAMNSPLAKALYGVDGVIRVFFGSDFVTVTK 136

Query: 121 SEDATWDFLKPEIFAAIMDFYSSGQPLFLDSKTAAAMDTAINEDDSEIVAMIKELLETRI 180
           S +A+WDFLKPEIFA+IMDFYSSGQPLFLD+ TAAAMDTAINEDDSE VAMIKELLETRI
Sbjct: 137 SNEASWDFLKPEIFASIMDFYSSGQPLFLDASTAAAMDTAINEDDSETVAMIKELLETRI 196

Query: 181 RPAVQDDGGDIEYRGYNEETGIVTLRMQGACSGCPSSSVTLKSGIENMLMHYVPEVKGVE 240
           RP VQDDGGDIEYRGYNEETGIV LRMQGACSGCPSSSVTLKSGIENMLMHYVPEVKGVE
Sbjct: 197 RPTVQDDGGDIEYRGYNEETGIVKLRMQGACSGCPSSSVTLKSGIENMLMHYVPEVKGVE 256

Query: 241 QEFDVEEEDAISTS 255
           QEFDVEEED  STS
Sbjct: 257 QEFDVEEEDVKSTS 270

BLAST of Moc04g33290 vs. NCBI nr
Match: XP_022992592.1 (nifU-like protein 4, mitochondrial [Cucurbita maxima])

HSP 1 Score: 446.8 bits (1148), Expect = 1.2e-121
Identity = 219/254 (86.22%), Postives = 238/254 (93.70%), Query Frame = 0

Query: 1   MDSRGAGKIRRISTATTSSLFGGEDAASCSTTSRSCSNSYLRSWKWNQFGGQRRTMFIQT 60
           + S G GKIR  S ATTSS+FGG++AASCS +SR+CS+ +LRSW W+QFGGQ+RTMFI+T
Sbjct: 17  LGSHGGGKIRFFSAATTSSIFGGKEAASCSVSSRTCSDPFLRSWSWSQFGGQKRTMFIET 76

Query: 61  QSTPNPSSLMFHPGKPVMEVGSADFPNARSAMNSPLAKALYGIDGVVRVFFGSDFVTVTK 120
           QSTPNPSSLMF+PGKPVMEVGSADFPNAR+AMNSPLAKALYG+DGV+RVFFGSDFVTVTK
Sbjct: 77  QSTPNPSSLMFYPGKPVMEVGSADFPNARTAMNSPLAKALYGVDGVIRVFFGSDFVTVTK 136

Query: 121 SEDATWDFLKPEIFAAIMDFYSSGQPLFLDSKTAAAMDTAINEDDSEIVAMIKELLETRI 180
           S +A+WDFLKPEIFA+IMDFYSSGQPLFLD+ TAAAMDTAINEDDSE VAMIKELLETRI
Sbjct: 137 SNEASWDFLKPEIFASIMDFYSSGQPLFLDANTAAAMDTAINEDDSETVAMIKELLETRI 196

Query: 181 RPAVQDDGGDIEYRGYNEETGIVTLRMQGACSGCPSSSVTLKSGIENMLMHYVPEVKGVE 240
           RP VQDDGGDIEYRGYNEETGIV LRMQGACSGCPSSSVTLKSGIENMLMHYVPEVKGVE
Sbjct: 197 RPTVQDDGGDIEYRGYNEETGIVKLRMQGACSGCPSSSVTLKSGIENMLMHYVPEVKGVE 256

Query: 241 QEFDVEEEDAISTS 255
           QEFDVEEED  STS
Sbjct: 257 QEFDVEEEDVKSTS 270

BLAST of Moc04g33290 vs. ExPASy Swiss-Prot
Match: Q9LIG6 (NifU-like protein 4, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=NIFU4 PE=2 SV=1)

HSP 1 Score: 362.5 bits (929), Expect = 4.0e-99
Identity = 179/212 (84.43%), Postives = 194/212 (91.51%), Query Frame = 0

Query: 38  NSYLRSWKWNQFGGQRRTMFIQTQSTPNPSSLMFHPGKPVMEVGSADFPNARSAMNSPLA 97
           NS L + KWN  GGQRRTMFIQTQSTPNPSSLMF+PGKPVMEVGSADFPN RSA+ SPLA
Sbjct: 62  NSSLLAQKWNFLGGQRRTMFIQTQSTPNPSSLMFYPGKPVMEVGSADFPNVRSALGSPLA 121

Query: 98  KALYGIDGVVRVFFGSDFVTVTKSEDATWDFLKPEIFAAIMDFYSSGQPLFLDSKTAAAM 157
           K++Y IDGVVRVFFGSDFVTVTKS+D +WD LKPEIFAA+MDFYSSGQPLFLDS+ AAA 
Sbjct: 122 KSIYSIDGVVRVFFGSDFVTVTKSDDVSWDILKPEIFAAVMDFYSSGQPLFLDSQAAAAK 181

Query: 158 DTAINEDDSEIVAMIKELLETRIRPAVQDDGGDIEYRGYNEETGIVTLRMQGACSGCPSS 217
           DTAI+EDDSE VAMIKELLETRIRPAVQDDGGDIEY G++ E+GIV LRMQGACSGCPSS
Sbjct: 182 DTAISEDDSETVAMIKELLETRIRPAVQDDGGDIEYCGFDPESGIVKLRMQGACSGCPSS 241

Query: 218 SVTLKSGIENMLMHYVPEVKGVEQEFDVEEED 250
           SVTLKSGIENMLMHYV EVKGVEQEFD E+E+
Sbjct: 242 SVTLKSGIENMLMHYVSEVKGVEQEFDGEDEE 273

BLAST of Moc04g33290 vs. ExPASy Swiss-Prot
Match: Q9C8J2 (NifU-like protein 5, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=NIFU5 PE=2 SV=1)

HSP 1 Score: 349.4 bits (895), Expect = 3.5e-95
Identity = 176/223 (78.92%), Postives = 194/223 (87.00%), Query Frame = 0

Query: 30  STTSRSCSNSYLRSWKWNQFGGQRRTMFIQTQSTPNPSSLMFHPGKPVMEVGSADFPNAR 89
           +  SR+CS S    + W +   QRRTMFIQTQSTPNPSSLMF PGKPVME+GSADFPN+R
Sbjct: 52  TNASRNCSRSSSFPFNWIE---QRRTMFIQTQSTPNPSSLMFSPGKPVMEIGSADFPNSR 111

Query: 90  SAMNSPLAKALYGIDGVVRVFFGSDFVTVTKSEDATWDFLKPEIFAAIMDFYSSGQPLFL 149
           SAM+SPLAKA++ IDGVVRVF+GSDFVTVTKS+D TWD LKP+IFA +MDFYSSGQPLFL
Sbjct: 112 SAMSSPLAKAIFAIDGVVRVFYGSDFVTVTKSDDVTWDILKPDIFAVVMDFYSSGQPLFL 171

Query: 150 DSKTAAAMDTAINEDDSEIVAMIKELLETRIRPAVQDDGGDIEYRGYNEETGIVTLRMQG 209
           DS+  AA DTAI+EDDSE VAMIKELLETRIRP+VQDDGGDIEY G++ ETGIV LRMQG
Sbjct: 172 DSQATAAKDTAIHEDDSETVAMIKELLETRIRPSVQDDGGDIEYCGFDTETGIVKLRMQG 231

Query: 210 ACSGCPSSSVTLKSGIENMLMHYVPEVKGVEQEFDVEEEDAIS 253
           ACSGCPSSSVTLKSGIENMLMHYV EVKGVEQEFD EEE   S
Sbjct: 232 ACSGCPSSSVTLKSGIENMLMHYVSEVKGVEQEFDGEEEGTSS 271

BLAST of Moc04g33290 vs. ExPASy Swiss-Prot
Match: Q8SY96 (NFU1 iron-sulfur cluster scaffold homolog, mitochondrial OS=Drosophila melanogaster OX=7227 GN=CG32500 PE=2 SV=1)

HSP 1 Score: 232.6 bits (592), Expect = 4.8e-60
Identity = 117/193 (60.62%), Postives = 145/193 (75.13%), Query Frame = 0

Query: 53  RRTMFIQTQSTPNPSSLMFHPGKPVMEVGSA-DFPNARSAMNSPLAKALYGIDGVVRVFF 112
           RR+MFIQTQ TPNP SL F PG  V+  G+  DFPN  +A NSPLAK L+ ++GV  VFF
Sbjct: 64  RRSMFIQTQDTPNPESLKFLPGVDVLGKGNTYDFPNGTTAHNSPLAKLLFRVEGVKGVFF 123

Query: 113 GSDFVTVTKSEDATWDFLKPEIFAAIMDFYSSGQPLFLDSKTAAAMDTAINEDDSEIVAM 172
           G+DFVT++K E A W  +KPE+FA IMDF++SG P+  D++  A  DT I EDD E V M
Sbjct: 124 GADFVTISKQEGAEWSLIKPEVFAVIMDFFASGLPVLNDAQPNA--DTEILEDDDETVMM 183

Query: 173 IKELLETRIRPAVQDDGGDIEYRGYNEETGIVTLRMQGACSGCPSSSVTLKSGIENMLMH 232
           IKELL+TRIRP VQ+DGGDI + GY  E G+V L+MQG+CS CPSS VTLK+G++NML  
Sbjct: 184 IKELLDTRIRPTVQEDGGDIVFMGY--EGGVVKLKMQGSCSSCPSSIVTLKNGVQNMLQF 243

Query: 233 YVPEVKGVEQEFD 245
           Y+PEV+ VEQ FD
Sbjct: 244 YIPEVESVEQVFD 252

BLAST of Moc04g33290 vs. ExPASy Swiss-Prot
Match: B3NYF7 (NFU1 iron-sulfur cluster scaffold homolog, mitochondrial OS=Drosophila erecta OX=7220 GN=GG17526 PE=3 SV=1)

HSP 1 Score: 231.5 bits (589), Expect = 1.1e-59
Identity = 116/193 (60.10%), Postives = 145/193 (75.13%), Query Frame = 0

Query: 53  RRTMFIQTQSTPNPSSLMFHPGKPVMEVGSA-DFPNARSAMNSPLAKALYGIDGVVRVFF 112
           RR+MFIQTQ TPNP SL F PG  V+  G+  DFPN  +A +SPLAK L+ ++GV  VFF
Sbjct: 64  RRSMFIQTQDTPNPDSLKFLPGVDVLGKGNTYDFPNGTTAHSSPLAKLLFRVEGVKGVFF 123

Query: 113 GSDFVTVTKSEDATWDFLKPEIFAAIMDFYSSGQPLFLDSKTAAAMDTAINEDDSEIVAM 172
           G+DFVT++K E A W  +KPE+FA IMDF++SG P+  D++  A  DT I EDD E V M
Sbjct: 124 GADFVTISKQEGAEWSLIKPEVFAVIMDFFASGLPVLHDAQPNA--DTEILEDDDETVMM 183

Query: 173 IKELLETRIRPAVQDDGGDIEYRGYNEETGIVTLRMQGACSGCPSSSVTLKSGIENMLMH 232
           IKELL+TRIRP VQ+DGGDI + GY  E G+V L+MQG+CS CPSS VTLK+G++NML  
Sbjct: 184 IKELLDTRIRPTVQEDGGDIVFMGY--EAGVVKLKMQGSCSSCPSSIVTLKNGVQNMLQF 243

Query: 233 YVPEVKGVEQEFD 245
           Y+PEV+ VEQ FD
Sbjct: 244 YIPEVESVEQVFD 252

BLAST of Moc04g33290 vs. ExPASy Swiss-Prot
Match: B4PZ52 (NFU1 iron-sulfur cluster scaffold homolog, mitochondrial OS=Drosophila yakuba OX=7245 GN=GE15286 PE=3 SV=1)

HSP 1 Score: 231.5 bits (589), Expect = 1.1e-59
Identity = 116/193 (60.10%), Postives = 145/193 (75.13%), Query Frame = 0

Query: 53  RRTMFIQTQSTPNPSSLMFHPGKPVMEVGSA-DFPNARSAMNSPLAKALYGIDGVVRVFF 112
           RR+MFIQTQ TPNP SL F PG  V+  G+  DFPN  +A +SPLAK L+ ++GV  VFF
Sbjct: 64  RRSMFIQTQDTPNPDSLKFLPGVDVLGKGNTYDFPNGTTAHSSPLAKLLFRVEGVKGVFF 123

Query: 113 GSDFVTVTKSEDATWDFLKPEIFAAIMDFYSSGQPLFLDSKTAAAMDTAINEDDSEIVAM 172
           G+DFVT++K E A W  +KPE+FA IMDF++SG P+  D++  A  DT I EDD E V M
Sbjct: 124 GADFVTISKQEGAEWSLIKPEVFAVIMDFFASGLPVLHDAQPNA--DTEILEDDDETVMM 183

Query: 173 IKELLETRIRPAVQDDGGDIEYRGYNEETGIVTLRMQGACSGCPSSSVTLKSGIENMLMH 232
           IKELL+TRIRP VQ+DGGDI + GY  E G+V L+MQG+CS CPSS VTLK+G++NML  
Sbjct: 184 IKELLDTRIRPTVQEDGGDIVFMGY--EAGVVKLKMQGSCSSCPSSIVTLKNGVQNMLQF 243

Query: 233 YVPEVKGVEQEFD 245
           Y+PEV+ VEQ FD
Sbjct: 244 YIPEVESVEQVFD 252

BLAST of Moc04g33290 vs. ExPASy TrEMBL
Match: A0A6J1BW08 (nifU-like protein 4, mitochondrial OS=Momordica charantia OX=3673 GN=LOC111006227 PE=3 SV=1)

HSP 1 Score: 504.2 bits (1297), Expect = 3.2e-139
Identity = 254/254 (100.00%), Postives = 254/254 (100.00%), Query Frame = 0

Query: 1   MDSRGAGKIRRISTATTSSLFGGEDAASCSTTSRSCSNSYLRSWKWNQFGGQRRTMFIQT 60
           MDSRGAGKIRRISTATTSSLFGGEDAASCSTTSRSCSNSYLRSWKWNQFGGQRRTMFIQT
Sbjct: 17  MDSRGAGKIRRISTATTSSLFGGEDAASCSTTSRSCSNSYLRSWKWNQFGGQRRTMFIQT 76

Query: 61  QSTPNPSSLMFHPGKPVMEVGSADFPNARSAMNSPLAKALYGIDGVVRVFFGSDFVTVTK 120
           QSTPNPSSLMFHPGKPVMEVGSADFPNARSAMNSPLAKALYGIDGVVRVFFGSDFVTVTK
Sbjct: 77  QSTPNPSSLMFHPGKPVMEVGSADFPNARSAMNSPLAKALYGIDGVVRVFFGSDFVTVTK 136

Query: 121 SEDATWDFLKPEIFAAIMDFYSSGQPLFLDSKTAAAMDTAINEDDSEIVAMIKELLETRI 180
           SEDATWDFLKPEIFAAIMDFYSSGQPLFLDSKTAAAMDTAINEDDSEIVAMIKELLETRI
Sbjct: 137 SEDATWDFLKPEIFAAIMDFYSSGQPLFLDSKTAAAMDTAINEDDSEIVAMIKELLETRI 196

Query: 181 RPAVQDDGGDIEYRGYNEETGIVTLRMQGACSGCPSSSVTLKSGIENMLMHYVPEVKGVE 240
           RPAVQDDGGDIEYRGYNEETGIVTLRMQGACSGCPSSSVTLKSGIENMLMHYVPEVKGVE
Sbjct: 197 RPAVQDDGGDIEYRGYNEETGIVTLRMQGACSGCPSSSVTLKSGIENMLMHYVPEVKGVE 256

Query: 241 QEFDVEEEDAISTS 255
           QEFDVEEEDAISTS
Sbjct: 257 QEFDVEEEDAISTS 270

BLAST of Moc04g33290 vs. ExPASy TrEMBL
Match: A0A6J1H8H2 (nifU-like protein 4, mitochondrial OS=Cucurbita moschata OX=3662 GN=LOC111461505 PE=3 SV=1)

HSP 1 Score: 448.7 bits (1153), Expect = 1.6e-122
Identity = 225/254 (88.58%), Postives = 241/254 (94.88%), Query Frame = 0

Query: 1   MDSRGAGKIRRISTATTSSLFGGEDAASCSTTSRSCSNSYLRSWKWNQFGGQRRTMFIQT 60
           ++SRG GKIR +S A TSSLF GE+AAS S +SR+ S+SYLRSWKWN+FGGQRRTMFI+T
Sbjct: 17  LNSRGVGKIRFVSAAATSSLFRGEEAASDSVSSRTFSDSYLRSWKWNRFGGQRRTMFIET 76

Query: 61  QSTPNPSSLMFHPGKPVMEVGSADFPNARSAMNSPLAKALYGIDGVVRVFFGSDFVTVTK 120
           QSTPNPSSLMF+PGKPVMEVGSADFPNARSAMNSPLA+ALYG+DGVVRVFFGSDFVTVTK
Sbjct: 77  QSTPNPSSLMFYPGKPVMEVGSADFPNARSAMNSPLARALYGVDGVVRVFFGSDFVTVTK 136

Query: 121 SEDATWDFLKPEIFAAIMDFYSSGQPLFLDSKTAAAMDTAINEDDSEIVAMIKELLETRI 180
           S DA+WDFLKPEIFAAIMDFYSSGQPLFLDS+TAAAMDTAINEDDSE VAMIKELLETRI
Sbjct: 137 SNDASWDFLKPEIFAAIMDFYSSGQPLFLDSQTAAAMDTAINEDDSETVAMIKELLETRI 196

Query: 181 RPAVQDDGGDIEYRGYNEETGIVTLRMQGACSGCPSSSVTLKSGIENMLMHYVPEVKGVE 240
           RPAVQDDGGDIEYRGY+EETGIV LRMQGACSGCPSSSVTLKSGIENMLMHYVPEVKGVE
Sbjct: 197 RPAVQDDGGDIEYRGYDEETGIVKLRMQGACSGCPSSSVTLKSGIENMLMHYVPEVKGVE 256

Query: 241 QEFDVEEEDAISTS 255
           QEFDVEE+D  STS
Sbjct: 257 QEFDVEEDDIKSTS 270

BLAST of Moc04g33290 vs. ExPASy TrEMBL
Match: A0A6J1JQB1 (nifU-like protein 4, mitochondrial OS=Cucurbita maxima OX=3661 GN=LOC111488894 PE=3 SV=1)

HSP 1 Score: 446.8 bits (1148), Expect = 6.0e-122
Identity = 219/254 (86.22%), Postives = 238/254 (93.70%), Query Frame = 0

Query: 1   MDSRGAGKIRRISTATTSSLFGGEDAASCSTTSRSCSNSYLRSWKWNQFGGQRRTMFIQT 60
           + S G GKIR  S ATTSS+FGG++AASCS +SR+CS+ +LRSW W+QFGGQ+RTMFI+T
Sbjct: 17  LGSHGGGKIRFFSAATTSSIFGGKEAASCSVSSRTCSDPFLRSWSWSQFGGQKRTMFIET 76

Query: 61  QSTPNPSSLMFHPGKPVMEVGSADFPNARSAMNSPLAKALYGIDGVVRVFFGSDFVTVTK 120
           QSTPNPSSLMF+PGKPVMEVGSADFPNAR+AMNSPLAKALYG+DGV+RVFFGSDFVTVTK
Sbjct: 77  QSTPNPSSLMFYPGKPVMEVGSADFPNARTAMNSPLAKALYGVDGVIRVFFGSDFVTVTK 136

Query: 121 SEDATWDFLKPEIFAAIMDFYSSGQPLFLDSKTAAAMDTAINEDDSEIVAMIKELLETRI 180
           S +A+WDFLKPEIFA+IMDFYSSGQPLFLD+ TAAAMDTAINEDDSE VAMIKELLETRI
Sbjct: 137 SNEASWDFLKPEIFASIMDFYSSGQPLFLDANTAAAMDTAINEDDSETVAMIKELLETRI 196

Query: 181 RPAVQDDGGDIEYRGYNEETGIVTLRMQGACSGCPSSSVTLKSGIENMLMHYVPEVKGVE 240
           RP VQDDGGDIEYRGYNEETGIV LRMQGACSGCPSSSVTLKSGIENMLMHYVPEVKGVE
Sbjct: 197 RPTVQDDGGDIEYRGYNEETGIVKLRMQGACSGCPSSSVTLKSGIENMLMHYVPEVKGVE 256

Query: 241 QEFDVEEEDAISTS 255
           QEFDVEEED  STS
Sbjct: 257 QEFDVEEEDVKSTS 270

BLAST of Moc04g33290 vs. ExPASy TrEMBL
Match: A0A6J1JTA1 (nifU-like protein 4, mitochondrial OS=Cucurbita maxima OX=3661 GN=LOC111487295 PE=3 SV=1)

HSP 1 Score: 446.4 bits (1147), Expect = 7.8e-122
Identity = 222/254 (87.40%), Postives = 239/254 (94.09%), Query Frame = 0

Query: 1   MDSRGAGKIRRISTATTSSLFGGEDAASCSTTSRSCSNSYLRSWKWNQFGGQRRTMFIQT 60
           ++ RG GKIR +S A TSSLF GE+AAS S +SR+CS+SYLRSWKWN+FGGQRRTMFI+T
Sbjct: 17  LNPRGVGKIRFVSAAATSSLFRGEEAASDSVSSRTCSDSYLRSWKWNRFGGQRRTMFIET 76

Query: 61  QSTPNPSSLMFHPGKPVMEVGSADFPNARSAMNSPLAKALYGIDGVVRVFFGSDFVTVTK 120
           QSTPNPSSLMF+PGKPVMEVGSADFPNARSAMNSPLA+ALYG+DGVVRVFFGSDFVTV K
Sbjct: 77  QSTPNPSSLMFYPGKPVMEVGSADFPNARSAMNSPLARALYGVDGVVRVFFGSDFVTVRK 136

Query: 121 SEDATWDFLKPEIFAAIMDFYSSGQPLFLDSKTAAAMDTAINEDDSEIVAMIKELLETRI 180
           S DA+WDFLKPEIFAAIMDFYSSGQPLFLDS+TAAAMDTAINEDDSE VAMIKELLETRI
Sbjct: 137 SNDASWDFLKPEIFAAIMDFYSSGQPLFLDSQTAAAMDTAINEDDSETVAMIKELLETRI 196

Query: 181 RPAVQDDGGDIEYRGYNEETGIVTLRMQGACSGCPSSSVTLKSGIENMLMHYVPEVKGVE 240
           RPAVQDDGGDIEYRGY+EETGIV LRMQGACSGCPSSSVTLKSGIENMLMHYVPEVKGVE
Sbjct: 197 RPAVQDDGGDIEYRGYDEETGIVNLRMQGACSGCPSSSVTLKSGIENMLMHYVPEVKGVE 256

Query: 241 QEFDVEEEDAISTS 255
           Q FD+EE+D  STS
Sbjct: 257 QVFDIEEDDVKSTS 270

BLAST of Moc04g33290 vs. ExPASy TrEMBL
Match: A0A6J1FIB0 (nifU-like protein 4, mitochondrial OS=Cucurbita moschata OX=3662 GN=LOC111445668 PE=3 SV=1)

HSP 1 Score: 444.5 bits (1142), Expect = 3.0e-121
Identity = 219/254 (86.22%), Postives = 236/254 (92.91%), Query Frame = 0

Query: 1   MDSRGAGKIRRISTATTSSLFGGEDAASCSTTSRSCSNSYLRSWKWNQFGGQRRTMFIQT 60
           + S G GKIR  S ATTSS+FGG +AASCS +SR+C +  LRSW+W+QFGGQ+RTMFI+T
Sbjct: 17  LGSHGVGKIRFFSAATTSSIFGGNEAASCSVSSRTCFDPLLRSWRWSQFGGQKRTMFIET 76

Query: 61  QSTPNPSSLMFHPGKPVMEVGSADFPNARSAMNSPLAKALYGIDGVVRVFFGSDFVTVTK 120
           QSTPNPSSLMF+PGKPVMEVGSADFPNARSAMNSPLAKALYG+DGV+RVFFGSDFVTVTK
Sbjct: 77  QSTPNPSSLMFYPGKPVMEVGSADFPNARSAMNSPLAKALYGVDGVIRVFFGSDFVTVTK 136

Query: 121 SEDATWDFLKPEIFAAIMDFYSSGQPLFLDSKTAAAMDTAINEDDSEIVAMIKELLETRI 180
           S +A+WDFLKPEIFA+IMDFYSSGQPLFLD+ TAAAMDTAINEDDSE VAMIKELLETRI
Sbjct: 137 SNEASWDFLKPEIFASIMDFYSSGQPLFLDANTAAAMDTAINEDDSETVAMIKELLETRI 196

Query: 181 RPAVQDDGGDIEYRGYNEETGIVTLRMQGACSGCPSSSVTLKSGIENMLMHYVPEVKGVE 240
           RP VQDDGGDIEYRGYNEETGIV LRMQGACSGCPSSSVTLKSGIENMLMHYVPEVKGVE
Sbjct: 197 RPTVQDDGGDIEYRGYNEETGIVKLRMQGACSGCPSSSVTLKSGIENMLMHYVPEVKGVE 256

Query: 241 QEFDVEEEDAISTS 255
           QEFDVEEED  STS
Sbjct: 257 QEFDVEEEDVKSTS 270

BLAST of Moc04g33290 vs. TAIR 10
Match: AT3G20970.1 (NFU domain protein 4 )

HSP 1 Score: 362.5 bits (929), Expect = 2.8e-100
Identity = 179/212 (84.43%), Postives = 194/212 (91.51%), Query Frame = 0

Query: 38  NSYLRSWKWNQFGGQRRTMFIQTQSTPNPSSLMFHPGKPVMEVGSADFPNARSAMNSPLA 97
           NS L + KWN  GGQRRTMFIQTQSTPNPSSLMF+PGKPVMEVGSADFPN RSA+ SPLA
Sbjct: 62  NSSLLAQKWNFLGGQRRTMFIQTQSTPNPSSLMFYPGKPVMEVGSADFPNVRSALGSPLA 121

Query: 98  KALYGIDGVVRVFFGSDFVTVTKSEDATWDFLKPEIFAAIMDFYSSGQPLFLDSKTAAAM 157
           K++Y IDGVVRVFFGSDFVTVTKS+D +WD LKPEIFAA+MDFYSSGQPLFLDS+ AAA 
Sbjct: 122 KSIYSIDGVVRVFFGSDFVTVTKSDDVSWDILKPEIFAAVMDFYSSGQPLFLDSQAAAAK 181

Query: 158 DTAINEDDSEIVAMIKELLETRIRPAVQDDGGDIEYRGYNEETGIVTLRMQGACSGCPSS 217
           DTAI+EDDSE VAMIKELLETRIRPAVQDDGGDIEY G++ E+GIV LRMQGACSGCPSS
Sbjct: 182 DTAISEDDSETVAMIKELLETRIRPAVQDDGGDIEYCGFDPESGIVKLRMQGACSGCPSS 241

Query: 218 SVTLKSGIENMLMHYVPEVKGVEQEFDVEEED 250
           SVTLKSGIENMLMHYV EVKGVEQEFD E+E+
Sbjct: 242 SVTLKSGIENMLMHYVSEVKGVEQEFDGEDEE 273

BLAST of Moc04g33290 vs. TAIR 10
Match: AT1G51390.1 (NFU domain protein 5 )

HSP 1 Score: 349.4 bits (895), Expect = 2.5e-96
Identity = 176/223 (78.92%), Postives = 194/223 (87.00%), Query Frame = 0

Query: 30  STTSRSCSNSYLRSWKWNQFGGQRRTMFIQTQSTPNPSSLMFHPGKPVMEVGSADFPNAR 89
           +  SR+CS S    + W +   QRRTMFIQTQSTPNPSSLMF PGKPVME+GSADFPN+R
Sbjct: 52  TNASRNCSRSSSFPFNWIE---QRRTMFIQTQSTPNPSSLMFSPGKPVMEIGSADFPNSR 111

Query: 90  SAMNSPLAKALYGIDGVVRVFFGSDFVTVTKSEDATWDFLKPEIFAAIMDFYSSGQPLFL 149
           SAM+SPLAKA++ IDGVVRVF+GSDFVTVTKS+D TWD LKP+IFA +MDFYSSGQPLFL
Sbjct: 112 SAMSSPLAKAIFAIDGVVRVFYGSDFVTVTKSDDVTWDILKPDIFAVVMDFYSSGQPLFL 171

Query: 150 DSKTAAAMDTAINEDDSEIVAMIKELLETRIRPAVQDDGGDIEYRGYNEETGIVTLRMQG 209
           DS+  AA DTAI+EDDSE VAMIKELLETRIRP+VQDDGGDIEY G++ ETGIV LRMQG
Sbjct: 172 DSQATAAKDTAIHEDDSETVAMIKELLETRIRPSVQDDGGDIEYCGFDTETGIVKLRMQG 231

Query: 210 ACSGCPSSSVTLKSGIENMLMHYVPEVKGVEQEFDVEEEDAIS 253
           ACSGCPSSSVTLKSGIENMLMHYV EVKGVEQEFD EEE   S
Sbjct: 232 ACSGCPSSSVTLKSGIENMLMHYVSEVKGVEQEFDGEEEGTSS 271

BLAST of Moc04g33290 vs. TAIR 10
Match: AT3G20970.2 (NFU domain protein 4 )

HSP 1 Score: 274.2 bits (700), Expect = 1.0e-73
Identity = 134/160 (83.75%), Postives = 146/160 (91.25%), Query Frame = 0

Query: 38  NSYLRSWKWNQFGGQRRTMFIQTQSTPNPSSLMFHPGKPVMEVGSADFPNARSAMNSPLA 97
           NS L + KWN  GGQRRTMFIQTQSTPNPSSLMF+PGKPVMEVGSADFPN RSA+ SPLA
Sbjct: 62  NSSLLAQKWNFLGGQRRTMFIQTQSTPNPSSLMFYPGKPVMEVGSADFPNVRSALGSPLA 121

Query: 98  KALYGIDGVVRVFFGSDFVTVTKSEDATWDFLKPEIFAAIMDFYSSGQPLFLDSKTAAAM 157
           K++Y IDGVVRVFFGSDFVTVTKS+D +WD LKPEIFAA+MDFYSSGQPLFLDS+ AAA 
Sbjct: 122 KSIYSIDGVVRVFFGSDFVTVTKSDDVSWDILKPEIFAAVMDFYSSGQPLFLDSQAAAAK 181

Query: 158 DTAINEDDSEIVAMIKELLETRIRPAVQDDGGDIEYRGYN 198
           DTAI+EDDSE VAMIKELLETRIRPAVQDDGGDIEY G++
Sbjct: 182 DTAISEDDSETVAMIKELLETRIRPAVQDDGGDIEYCGFD 221

BLAST of Moc04g33290 vs. TAIR 10
Match: AT5G49940.1 (NIFU-like protein 2 )

HSP 1 Score: 62.4 bits (150), Expect = 6.1e-10
Identity = 40/109 (36.70%), Postives = 60/109 (55.05%), Query Frame = 0

Query: 141 YSSGQPLFLDSKTAAAMDTAINEDDSEIVAMIKELLET---RIRPAVQDDGGDIEYRGYN 200
           + S + LF  SK   A+ T    D    V + +E +E+    IRP +  DGG++    + 
Sbjct: 58  FLSSRQLFRRSKVVKAVAT---PDPILEVPLTEENVESVLDEIRPYLMSDGGNVAL--HE 117

Query: 201 EETGIVTLRMQGACSGCPSSSVTLKSGIENMLMHYVPEVKGVEQEFDVE 247
            +  IV +++QGAC  CPSS++T+K GIE  LM  +PE+  VE   D E
Sbjct: 118 IDGNIVRVKLQGACGSCPSSTMTMKMGIERRLMEKIPEIVAVEALPDEE 161

BLAST of Moc04g33290 vs. TAIR 10
Match: AT5G49940.2 (NIFU-like protein 2 )

HSP 1 Score: 62.4 bits (150), Expect = 6.1e-10
Identity = 40/109 (36.70%), Postives = 60/109 (55.05%), Query Frame = 0

Query: 141 YSSGQPLFLDSKTAAAMDTAINEDDSEIVAMIKELLET---RIRPAVQDDGGDIEYRGYN 200
           + S + LF  SK   A+ T    D    V + +E +E+    IRP +  DGG++    + 
Sbjct: 58  FLSSRQLFRRSKVVKAVAT---PDPILEVPLTEENVESVLDEIRPYLMSDGGNVAL--HE 117

Query: 201 EETGIVTLRMQGACSGCPSSSVTLKSGIENMLMHYVPEVKGVEQEFDVE 247
            +  IV +++QGAC  CPSS++T+K GIE  LM  +PE+  VE   D E
Sbjct: 118 IDGNIVRVKLQGACGSCPSSTMTMKMGIERRLMEKIPEIVAVEALPDEE 161

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022133710.16.5e-139100.00nifU-like protein 4, mitochondrial [Momordica charantia][more]
XP_038884119.14.5e-12490.16nifU-like protein 4, mitochondrial [Benincasa hispida][more]
XP_022960817.13.2e-12288.58nifU-like protein 4, mitochondrial [Cucurbita moschata][more]
KAG6578816.19.4e-12286.61NifU-like protein 4, mitochondrial, partial [Cucurbita argyrosperma subsp. soror... [more]
XP_022992592.11.2e-12186.22nifU-like protein 4, mitochondrial [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
Q9LIG64.0e-9984.43NifU-like protein 4, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=NIFU4 PE=2... [more]
Q9C8J23.5e-9578.92NifU-like protein 5, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=NIFU5 PE=2... [more]
Q8SY964.8e-6060.62NFU1 iron-sulfur cluster scaffold homolog, mitochondrial OS=Drosophila melanogas... [more]
B3NYF71.1e-5960.10NFU1 iron-sulfur cluster scaffold homolog, mitochondrial OS=Drosophila erecta OX... [more]
B4PZ521.1e-5960.10NFU1 iron-sulfur cluster scaffold homolog, mitochondrial OS=Drosophila yakuba OX... [more]
Match NameE-valueIdentityDescription
A0A6J1BW083.2e-139100.00nifU-like protein 4, mitochondrial OS=Momordica charantia OX=3673 GN=LOC11100622... [more]
A0A6J1H8H21.6e-12288.58nifU-like protein 4, mitochondrial OS=Cucurbita moschata OX=3662 GN=LOC111461505... [more]
A0A6J1JQB16.0e-12286.22nifU-like protein 4, mitochondrial OS=Cucurbita maxima OX=3661 GN=LOC111488894 P... [more]
A0A6J1JTA17.8e-12287.40nifU-like protein 4, mitochondrial OS=Cucurbita maxima OX=3661 GN=LOC111487295 P... [more]
A0A6J1FIB03.0e-12186.22nifU-like protein 4, mitochondrial OS=Cucurbita moschata OX=3662 GN=LOC111445668... [more]
Match NameE-valueIdentityDescription
AT3G20970.12.8e-10084.43NFU domain protein 4 [more]
AT1G51390.12.5e-9678.92NFU domain protein 5 [more]
AT3G20970.21.0e-7383.75NFU domain protein 4 [more]
AT5G49940.16.1e-1036.70NIFU-like protein 2 [more]
AT5G49940.26.1e-1036.70NIFU-like protein 2 [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (OHB3-1) v2
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR014824Scaffold protein Nfu/NifU, N-terminalSMARTSM00932Nfu_N_3acoord: 58..144
e-value: 6.5E-46
score: 168.5
IPR014824Scaffold protein Nfu/NifU, N-terminalPFAMPF08712Nfu_Ncoord: 58..144
e-value: 6.8E-30
score: 103.0
IPR036498Scaffold protein Nfu/NifU, N-terminal domain superfamilyGENE3D3.30.1370.70coord: 47..150
e-value: 6.6E-37
score: 127.4
IPR036498Scaffold protein Nfu/NifU, N-terminal domain superfamilySUPERFAMILY110836Hypothetical protein SAV1430coord: 56..146
IPR001075NIF system FeS cluster assembly, NifU, C-terminalPFAMPF01106NifUcoord: 172..240
e-value: 5.2E-29
score: 100.2
IPR035433NFU1-likePIRSFPIRSF036773HIRIP5coord: 26..254
e-value: 1.0E-96
score: 320.6
IPR034904Fe-S cluster assembly domain superfamilyGENE3D3.30.300.130coord: 166..241
e-value: 9.7E-34
score: 117.2
IPR034904Fe-S cluster assembly domain superfamilySUPERFAMILY117916Fe-S cluster assembly (FSCA) domain-likecoord: 158..244
NoneNo IPR availablePANTHERPTHR11178IRON-SULFUR CLUSTER SCAFFOLD PROTEIN NFU-RELATEDcoord: 29..252
NoneNo IPR availablePANTHERPTHR11178:SF43NIFU-LIKE PROTEIN 5, MITOCHONDRIALcoord: 29..252

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Moc04g33290.1Moc04g33290.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0016226 iron-sulfur cluster assembly
biological_process GO:0097428 protein maturation by iron-sulfur cluster transfer
cellular_component GO:0005739 mitochondrion
molecular_function GO:0051539 4 iron, 4 sulfur cluster binding
molecular_function GO:0005506 iron ion binding
molecular_function GO:0051536 iron-sulfur cluster binding