Moc04g32650 (gene) Bitter gourd (OHB3-1) v2

Overview


Name	Moc04g32650
Type	gene
Organism	Momordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
Description	Hypoxanthine phosphoribosyltransferase
Location	chr4: 24566624 .. 24568608 (-)
RNA-Seq Expression	Moc04g32650
Synteny	Moc04g32650

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCGGTACACTCTAAAATTGAGACAGTGCTATGGAACCAGGACCAGATTTCGGCTCGAGTCGCCGACATCGCCTCCCGAATCTCTCTCGACTTCCCCGCATTCTCTTCGCCGCCGGTCCTCGTCGGCGTCGCCACCGGAGCTTTCGTCTTCCTCGCCGACTTGGTCCGTCACATCAACCTTCCAATTTCTGTTGACCTTGTTCGGGTTGAATCCTACGGTTTCGGCACTCACTCTAATGGCGCTCCGAGAATCTCGTCCGACTTGAAGGTCGATGTCAAGAACAAGCACGTAATTCTGGTATCTGCACAAAATCTTACCCGCTCCGTCTATGCTTTTGCCTAATACTTGTGCGTGTTCTGTTCGCCATTGAATCACTTGAATCGCTTGTTTTTGCCGATTGAAAATTTCGCATGTTTGATGGTCACGGTTTCATGGTCGCCATGAACTCTTTTTCGGGGATTTTCTTTGCTTTCGCCTTTATACTGATGCACGGTGGAGTTTGTGGTGACTGAGCGTACTTTAAGGAAATTTTGCATGTCAGGTTTCTACTATTATTAGGGTGGCGTGGGAACATAAAGAAGAAAGAATTTAAAAATTGGGGAGAAACAATTGCATAGGGTCGATGGAACTGAATTCCGAATTCTTCACATTTGGGTATATGTTTTAAAGGCGATTAAAATCATAGTCTAGATCAGATTATGTCATTTAAGACAATTATATAAACCTTATTAATTTGTACACTTTTTTTTTTTAATAAAATAAAAACAATATAATGTTCAATTTTCCTCATCATTTTCCTTCGGTTGATGTTAACTCCGTGAGTATCAACAGAGAGAAATTATGGTCTTTTGCTCCCTGTTTTCCACTTTTCGGGATCTCTGAATAGAAATGAATTGTAGATCCATTTTTTTTCCTTTTTAATTCTTACTAATTGGAGATCTATTTGTACTTTTCTTGACTTCGGGTGGAATTTTACCCTCTTCCCCTAGTACATCTTTTCTAGCTGTTGGCATATTGATTTGTTTCCTATCTAAAAGAAGAAGGGGGGAAGAAAGTAGATGAAAGATTCTCTCAACAACTTGTCTGGGCTGGGGGATTGCCCATTTGGAACATTTTGACGTTGGTTGATGCATCATTGAATTTCTATTTCTTGTTCATTTTCATATCTGTCTCTTAGATCTTAATCGTGTCTGATCTGTGACATCGAATTTGAGCTTATGGATCTTGCATGTAGGAAATCGTGGGATATGGACAGGGCAATGCCATGAATTAAATCTCCTTGAAACAGTAGGCTTTTGTTTCATGTTTTTGGGTATAAAATATGTGGCTTTTTCCATGTGTCAGGCTTTAATAGATTGATTGTCTTCCTGTATTTGGCATGGGTTATTGGGTTGACAAATGATAATTGACTGATACTTATCCATATATTTGAGAGTGATGAATTTCTCCCACAATTCTTCTTGTAAAGCACATTTAGCATCTTCTTGACTAATTTTGTAATGAATCTCCTATTAGGTTGAGGATATTGTAGACACTGGAAACACTTTATCGTGTCTCATTGCTCATATGGAGGCAAAAGGAGCATCATCTGTATCAGTCTGCACTTTTCTCGACAAACCAACGAGACGAAAGGTTCATTTTCAGCTAGTAGGCGAAGGAAAGTTCTACTGTGGGTTTGAAGTAAGATGAAATTGAATCGAACTTTTTTCCCCTTATAAGAATGCATATTAAACTAGAAACATTACCTGGCTTTATTCTTAATTGCATTGAAAATTTTCCAGCAAGTAGACAAATGAAAGTACCTCAAGTACCAATCACACGTTCTTCAGTTGCTATTACTTGATTCAGTTAAACCATTTTCTTATATAGTTTGTTTTGAACAGTGTCCAGATTACTTTGTCGTGGGCTACGGAATGGACTTTGCGGAGCAGTACAGAAATTTACCATATGTTGGCGTCTTGAAGCCCGAGTGTTACCAGTGA

mRNA sequence

ATGGCGGTACACTCTAAAATTGAGACAGTGCTATGGAACCAGGACCAGATTTCGGCTCGAGTCGCCGACATCGCCTCCCGAATCTCTCTCGACTTCCCCGCATTCTCTTCGCCGCCGGTCCTCGTCGGCGTCGCCACCGGAGCTTTCGTCTTCCTCGCCGACTTGGTCCGTCACATCAACCTTCCAATTTCTGTTGACCTTGTTCGGGTTGAATCCTACGGTTTCGGCACTCACTCTAATGGCGCTCCGAGAATCTCGTCCGACTTGAAGGTCGATGTCAAGAACAAGCACGTAATTCTGGTTGAGGATATTGTAGACACTGGAAACACTTTATCGTGTCTCATTGCTCATATGGAGGCAAAAGGAGCATCATCTGTATCAGTCTGCACTTTTCTCGACAAACCAACGAGACGAAAGGTTCATTTTCAGCTAGTAGGCGAAGGAAAGTTCTACTGTGGGTTTGAATGTCCAGATTACTTTGTCGTGGGCTACGGAATGGACTTTGCGGAGCAGTACAGAAATTTACCATATGTTGGCGTCTTGAAGCCCGAGTGTTACCAGTGA

Coding sequence (CDS)

ATGGCGGTACACTCTAAAATTGAGACAGTGCTATGGAACCAGGACCAGATTTCGGCTCGAGTCGCCGACATCGCCTCCCGAATCTCTCTCGACTTCCCCGCATTCTCTTCGCCGCCGGTCCTCGTCGGCGTCGCCACCGGAGCTTTCGTCTTCCTCGCCGACTTGGTCCGTCACATCAACCTTCCAATTTCTGTTGACCTTGTTCGGGTTGAATCCTACGGTTTCGGCACTCACTCTAATGGCGCTCCGAGAATCTCGTCCGACTTGAAGGTCGATGTCAAGAACAAGCACGTAATTCTGGTTGAGGATATTGTAGACACTGGAAACACTTTATCGTGTCTCATTGCTCATATGGAGGCAAAAGGAGCATCATCTGTATCAGTCTGCACTTTTCTCGACAAACCAACGAGACGAAAGGTTCATTTTCAGCTAGTAGGCGAAGGAAAGTTCTACTGTGGGTTTGAATGTCCAGATTACTTTGTCGTGGGCTACGGAATGGACTTTGCGGAGCAGTACAGAAATTTACCATATGTTGGCGTCTTGAAGCCCGAGTGTTACCAGTGA

Protein sequence

MAVHSKIETVLWNQDQISARVADIASRISLDFPAFSSPPVLVGVATGAFVFLADLVRHINLPISVDLVRVESYGFGTHSNGAPRISSDLKVDVKNKHVILVEDIVDTGNTLSCLIAHMEAKGASSVSVCTFLDKPTRRKVHFQLVGEGKFYCGFECPDYFVVGYGMDFAEQYRNLPYVGVLKPECYQ

Homology

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BLAST of Moc04g32650 vs. NCBI nr
Match: XP_022133463.1 (uncharacterized protein LOC111006034 [Momordica charantia])

HSP 1 Score: 382.9 bits (982), Expect = 1.6e-102
Identity = 187/187 (100.00%), Postives = 187/187 (100.00%), Query Frame = 0


Query: 1   MAVHSKIETVLWNQDQISARVADIASRISLDFPAFSSPPVLVGVATGAFVFLADLVRHIN 60
           MAVHSKIETVLWNQDQISARVADIASRISLDFPAFSSPPVLVGVATGAFVFLADLVRHIN
Sbjct: 1   MAVHSKIETVLWNQDQISARVADIASRISLDFPAFSSPPVLVGVATGAFVFLADLVRHIN 60

Query: 61  LPISVDLVRVESYGFGTHSNGAPRISSDLKVDVKNKHVILVEDIVDTGNTLSCLIAHMEA 120
           LPISVDLVRVESYGFGTHSNGAPRISSDLKVDVKNKHVILVEDIVDTGNTLSCLIAHMEA
Sbjct: 61  LPISVDLVRVESYGFGTHSNGAPRISSDLKVDVKNKHVILVEDIVDTGNTLSCLIAHMEA 120

Query: 121 KGASSVSVCTFLDKPTRRKVHFQLVGEGKFYCGFECPDYFVVGYGMDFAEQYRNLPYVGV 180
           KGASSVSVCTFLDKPTRRKVHFQLVGEGKFYCGFECPDYFVVGYGMDFAEQYRNLPYVGV
Sbjct: 121 KGASSVSVCTFLDKPTRRKVHFQLVGEGKFYCGFECPDYFVVGYGMDFAEQYRNLPYVGV 180

Query: 181 LKPECYQ 188
           LKPECYQ
Sbjct: 181 LKPECYQ 187

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BLAST of Moc04g32650 vs. NCBI nr
Match: XP_023527455.1 (uncharacterized protein LOC111790677 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 339.7 bits (870), Expect = 1.6e-89
Identity = 164/187 (87.70%), Postives = 175/187 (93.58%), Query Frame = 0


Query: 1   MAVHSKIETVLWNQDQISARVADIASRISLDFPAFSSPPVLVGVATGAFVFLADLVRHIN 60
           MA+HS IE VLWNQDQIS+RVAD+AS+IS DF   SSPPV+VGVATGAF+FLADLVR IN
Sbjct: 1   MALHSHIERVLWNQDQISSRVADLASQISADFSETSSPPVIVGVATGAFIFLADLVRRIN 60

Query: 61  LPISVDLVRVESYGFGTHSNGAPRISSDLKVDVKNKHVILVEDIVDTGNTLSCLIAHMEA 120
           LPI++D VRVESYG GT SNGAPRISSDLKVDVKNKHVILVEDIVD+GNTLSCL+AHMEA
Sbjct: 61  LPITLDFVRVESYGVGTESNGAPRISSDLKVDVKNKHVILVEDIVDSGNTLSCLLAHMEA 120

Query: 121 KGASSVSVCTFLDKPTRRKVHFQLVGEGKFYCGFECPDYFVVGYGMDFAEQYRNLPYVGV 180
           KGASSVSVCTFLDKPTRRKVHFQLVGEGKFY GFECPDYFVVGYGMDFAE YRNLPY+GV
Sbjct: 121 KGASSVSVCTFLDKPTRRKVHFQLVGEGKFYHGFECPDYFVVGYGMDFAELYRNLPYIGV 180

Query: 181 LKPECYQ 188
           LKPECYQ
Sbjct: 181 LKPECYQ 187

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BLAST of Moc04g32650 vs. NCBI nr
Match: KAA0031631.1 (hypoxanthine-guanine phosphoribosyltransferase [Cucumis melo var. makuwa] >TYK04477.1 hypoxanthine-guanine phosphoribosyltransferase [Cucumis melo var. makuwa])

HSP 1 Score: 338.6 bits (867), Expect = 3.5e-89
Identity = 163/187 (87.17%), Postives = 174/187 (93.05%), Query Frame = 0


Query: 1   MAVHSKIETVLWNQDQISARVADIASRISLDFPAFSSPPVLVGVATGAFVFLADLVRHIN 60
           MAVHS IE VLW  DQISARV+D+AS+IS+ F   SSPPVLVGVATGAF+FLADLVRHIN
Sbjct: 1   MAVHSHIERVLWTHDQISARVSDLASQISIAFHGASSPPVLVGVATGAFIFLADLVRHIN 60

Query: 61  LPISVDLVRVESYGFGTHSNGAPRISSDLKVDVKNKHVILVEDIVDTGNTLSCLIAHMEA 120
           +PI+VD VRVESYGFGTHSNGAP ISSDLKVDVKNKHVILVEDIVDTGNTLSCLIAHME 
Sbjct: 61  IPIAVDFVRVESYGFGTHSNGAPTISSDLKVDVKNKHVILVEDIVDTGNTLSCLIAHMEV 120

Query: 121 KGASSVSVCTFLDKPTRRKVHFQLVGEGKFYCGFECPDYFVVGYGMDFAEQYRNLPYVGV 180
           KGASSVSVCTFLDKPTRR+V+FQLVGEGKFY GF+CPDYFVVGYGMDFAE YRNLPYVG+
Sbjct: 121 KGASSVSVCTFLDKPTRRQVNFQLVGEGKFYSGFQCPDYFVVGYGMDFAELYRNLPYVGI 180

Query: 181 LKPECYQ 188
           LKPECYQ
Sbjct: 181 LKPECYQ 187

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BLAST of Moc04g32650 vs. NCBI nr
Match: XP_008456720.1 (PREDICTED: hypoxanthine-guanine phosphoribosyltransferase [Cucumis melo])

HSP 1 Score: 338.6 bits (867), Expect = 3.5e-89
Identity = 163/187 (87.17%), Postives = 174/187 (93.05%), Query Frame = 0


Query: 1   MAVHSKIETVLWNQDQISARVADIASRISLDFPAFSSPPVLVGVATGAFVFLADLVRHIN 60
           MAVHS IE VLW  DQISARV+D+AS+IS+ F   SSPPVLVGVATGAF+FLADLVRHIN
Sbjct: 20  MAVHSHIERVLWTHDQISARVSDLASQISIAFHGASSPPVLVGVATGAFIFLADLVRHIN 79

Query: 61  LPISVDLVRVESYGFGTHSNGAPRISSDLKVDVKNKHVILVEDIVDTGNTLSCLIAHMEA 120
           +PI+VD VRVESYGFGTHSNGAP ISSDLKVDVKNKHVILVEDIVDTGNTLSCLIAHME 
Sbjct: 80  IPIAVDFVRVESYGFGTHSNGAPTISSDLKVDVKNKHVILVEDIVDTGNTLSCLIAHMEV 139

Query: 121 KGASSVSVCTFLDKPTRRKVHFQLVGEGKFYCGFECPDYFVVGYGMDFAEQYRNLPYVGV 180
           KGASSVSVCTFLDKPTRR+V+FQLVGEGKFY GF+CPDYFVVGYGMDFAE YRNLPYVG+
Sbjct: 140 KGASSVSVCTFLDKPTRRQVNFQLVGEGKFYSGFQCPDYFVVGYGMDFAELYRNLPYVGI 199

Query: 181 LKPECYQ 188
           LKPECYQ
Sbjct: 200 LKPECYQ 206

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BLAST of Moc04g32650 vs. NCBI nr
Match: XP_022953780.1 (uncharacterized protein LOC111456203 [Cucurbita moschata])

HSP 1 Score: 337.8 bits (865), Expect = 5.9e-89
Identity = 164/187 (87.70%), Postives = 175/187 (93.58%), Query Frame = 0


Query: 1   MAVHSKIETVLWNQDQISARVADIASRISLDFPAFSSPPVLVGVATGAFVFLADLVRHIN 60
           MA+HS IE VLWNQDQISARVAD+AS+IS DF   SSPPV+VGVATGAF+FLADLVR IN
Sbjct: 1   MALHSHIERVLWNQDQISARVADLASQISADFSETSSPPVIVGVATGAFIFLADLVRLIN 60

Query: 61  LPISVDLVRVESYGFGTHSNGAPRISSDLKVDVKNKHVILVEDIVDTGNTLSCLIAHMEA 120
           LPI++D VRVESYG GT SNG+PRISSDLKVDVKNKHVILVEDIVD+GNTLSCL+AHMEA
Sbjct: 61  LPITLDSVRVESYGVGTESNGSPRISSDLKVDVKNKHVILVEDIVDSGNTLSCLLAHMEA 120

Query: 121 KGASSVSVCTFLDKPTRRKVHFQLVGEGKFYCGFECPDYFVVGYGMDFAEQYRNLPYVGV 180
           KGASSVSVCTFLDKPTRRKVHFQLVGEGKFY GFECPDYFVVGYGMDFAE YRNLPY+GV
Sbjct: 121 KGASSVSVCTFLDKPTRRKVHFQLVGEGKFYHGFECPDYFVVGYGMDFAELYRNLPYIGV 180

Query: 181 LKPECYQ 188
           LKPECYQ
Sbjct: 181 LKPECYQ 187

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BLAST of Moc04g32650 vs. ExPASy Swiss-Prot
Match: Q839B2 (Hypoxanthine-guanine phosphoribosyltransferase OS=Enterococcus faecalis (strain ATCC 700802 / V583) OX=226185 GN=hpt PE=3 SV=1)

HSP 1 Score: 153.7 bits (387), Expect = 2.1e-36
Identity = 77/185 (41.62%), Postives = 119/185 (64.32%), Query Frame = 0


Query: 3   VHSKIETVLWNQDQISARVADIASRISLDFPAFSSPPVLVGVATGAFVFLADLVRHINLP 62
           +   IE VL ++++I A+ A++  +++ ++      P++VG+  GA  F+ADL R IN  
Sbjct: 2   IEKDIEKVLISKEEILAKSAELGKQLTEEYQ--GKNPLVVGILKGAVPFMADLTREINTY 61

Query: 63  ISVDLVRVESYGFGTHSNGAPRISSDLKVDVKNKHVILVEDIVDTGNTLSCLIAHMEAKG 122
           + +D + V SYG  T S+G  +I  DL  +V+ +H+++VEDI+D+G TL+ L+     + 
Sbjct: 62  LELDFMDVSSYGNATVSSGEVKIVKDLDTNVEGRHILIVEDIIDSGRTLAYLVDLFRYRK 121

Query: 123 ASSVSVCTFLDKPTRRKVHFQLVGEGKFYCGFECPDYFVVGYGMDFAEQYRNLPYVGVLK 182
           A+SV + T LDKP  R V  +       Y GF+ P+ FVVGYG+D+AE YRNLPY+GVLK
Sbjct: 122 AASVKIVTLLDKPEGRVVDIK-----ADYVGFDVPNEFVVGYGLDYAETYRNLPYIGVLK 179

Query: 183 PECYQ 188
           PE Y+
Sbjct: 182 PEVYE 179

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BLAST of Moc04g32650 vs. ExPASy Swiss-Prot
Match: P37472 (Hypoxanthine-guanine phosphoribosyltransferase OS=Bacillus subtilis (strain 168) OX=224308 GN=hprT PE=3 SV=1)

HSP 1 Score: 150.6 bits (379), Expect = 1.8e-35
Identity = 73/181 (40.33%), Postives = 117/181 (64.64%), Query Frame = 0


Query: 7   IETVLWNQDQISARVADIASRISLDFPAFSSPPVLVGVATGAFVFLADLVRHINLPISVD 66
           IE VL ++++I  +V ++ + ++ ++    + P+ +GV  GA  F+ADL++HI+  + +D
Sbjct: 6   IEKVLISEEEIQKKVKELGAELTSEYQ--DTFPLAIGVLKGALPFMADLIKHIDTYLEMD 65

Query: 67  LVRVESYGFGTHSNGAPRISSDLKVDVKNKHVILVEDIVDTGNTLSCLIAHMEAKGASSV 126
            + V SYG  T S+G  +I  DL   V+ + ++++EDI+D+G TLS L+     + A S+
Sbjct: 66  FMDVSSYGNSTVSSGEVKIIKDLDTSVEGRDILIIEDIIDSGLTLSYLVELFRYRKAKSI 125

Query: 127 SVCTFLDKPTRRKVHFQLVGEGKFYCGFECPDYFVVGYGMDFAEQYRNLPYVGVLKPECY 186
            + T LDKP+ RK   +       + GFE PD FVVGYG+D+AE+YRNLPY+GVLKP  Y
Sbjct: 126 KIVTLLDKPSGRKADIK-----ADFVGFEVPDAFVVGYGLDYAERYRNLPYIGVLKPAVY 179

Query: 187 Q 188
           +
Sbjct: 186 E 179

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BLAST of Moc04g32650 vs. ExPASy Swiss-Prot
Match: Q92F56 (Bifunctional protein TilS/HprT OS=Listeria innocua serovar 6a (strain ATCC BAA-680 / CLIP 11262) OX=272626 GN=tilS/hprT PE=3 SV=1)

HSP 1 Score: 149.8 bits (377), Expect = 3.0e-35
Identity = 73/185 (39.46%), Postives = 120/185 (64.86%), Query Frame = 0


Query: 2   AVHSKIETVLWNQDQISARVADIASRISLDFPAFSSPPVLVGVATGAFVFLADLVRHINL 61
           ++H+ I+ VL ++D++  ++ ++   ++ ++   +  P++VGV  GA  F+ DL++ I+ 
Sbjct: 469 SMHNDIQKVLISEDELQEKIRELGRELTAEYEGRN--PLVVGVLKGATPFMTDLLKRIDT 528

Query: 62  PISVDLVRVESYGFGTHSNGAPRISSDLKVDVKNKHVILVEDIVDTGNTLSCLIAHMEAK 121
            + +D + V SYG GT S+G  +I  DL   V+ + V+++EDI+D+G TLS L+  ++ +
Sbjct: 529 YLEMDFMDVSSYGNGTVSSGEVKIIKDLNASVEGRDVLIIEDIIDSGRTLSYLVDLIKYR 588

Query: 122 GASSVSVCTFLDKPTRRKVHFQLVGEGKFYCGFECPDYFVVGYGMDFAEQYRNLPYVGVL 181
            A SV + T LDKP  R V  +       Y GF  P+ FVVGYG+D+AE+YRNLPY+G+L
Sbjct: 589 KAKSVKLVTLLDKPAGRNVAIE-----ADYVGFVVPNEFVVGYGLDYAERYRNLPYIGIL 646

Query: 182 KPECY 187
           KPE Y
Sbjct: 649 KPEIY 646

[Close]

BLAST of Moc04g32650 vs. ExPASy Swiss-Prot
Match: Q724J4 (Bifunctional protein TilS/HprT OS=Listeria monocytogenes serotype 4b (strain F2365) OX=265669 GN=tilS/hprT PE=3 SV=1)

HSP 1 Score: 149.8 bits (377), Expect = 3.0e-35
Identity = 72/185 (38.92%), Postives = 120/185 (64.86%), Query Frame = 0


Query: 2   AVHSKIETVLWNQDQISARVADIASRISLDFPAFSSPPVLVGVATGAFVFLADLVRHINL 61
           ++H+ I+ VL ++D++  ++ ++   ++ ++   +  P++VGV  GA  F+ DL++ ++ 
Sbjct: 469 SMHNDIQKVLISEDELQEKIRELGRELTTEYEGRN--PLVVGVLKGATPFMTDLLKRVDT 528

Query: 62  PISVDLVRVESYGFGTHSNGAPRISSDLKVDVKNKHVILVEDIVDTGNTLSCLIAHMEAK 121
            + +D + V SYG GT S+G  +I  DL   V+ + V+++EDI+D+G TLS L+  ++ +
Sbjct: 529 YLEMDFMDVSSYGNGTVSSGEVKIIKDLNASVEGRDVLVIEDIIDSGRTLSYLVDLIKYR 588

Query: 122 GASSVSVCTFLDKPTRRKVHFQLVGEGKFYCGFECPDYFVVGYGMDFAEQYRNLPYVGVL 181
            A SV + T LDKP  R V  +       Y GF  P+ FVVGYG+D+AE+YRNLPY+G+L
Sbjct: 589 KAKSVKLVTLLDKPAGRNVEIE-----ADYVGFVVPNEFVVGYGLDYAERYRNLPYIGIL 646

Query: 182 KPECY 187
           KPE Y
Sbjct: 649 KPEIY 646

[Close]

BLAST of Moc04g32650 vs. ExPASy Swiss-Prot
Match: Q8YAC7 (Bifunctional protein TilS/HprT OS=Listeria monocytogenes serovar 1/2a (strain ATCC BAA-679 / EGD-e) OX=169963 GN=tilS/hprT PE=3 SV=1)

HSP 1 Score: 149.8 bits (377), Expect = 3.0e-35
Identity = 72/185 (38.92%), Postives = 120/185 (64.86%), Query Frame = 0


Query: 2   AVHSKIETVLWNQDQISARVADIASRISLDFPAFSSPPVLVGVATGAFVFLADLVRHINL 61
           ++H+ I+ VL ++D++  ++ ++   ++ ++   +  P++VGV  GA  F+ DL++ ++ 
Sbjct: 469 SMHNDIQKVLISEDELQEKIRELGRELTTEYEGRN--PLVVGVLKGATPFMTDLLKRVDT 528

Query: 62  PISVDLVRVESYGFGTHSNGAPRISSDLKVDVKNKHVILVEDIVDTGNTLSCLIAHMEAK 121
            + +D + V SYG GT S+G  +I  DL   V+ + V+++EDI+D+G TLS L+  ++ +
Sbjct: 529 YLEMDFMDVSSYGNGTVSSGEVKIIKDLNASVEGRDVLVIEDIIDSGRTLSYLVDLIKYR 588

Query: 122 GASSVSVCTFLDKPTRRKVHFQLVGEGKFYCGFECPDYFVVGYGMDFAEQYRNLPYVGVL 181
            A SV + T LDKP  R V  +       Y GF  P+ FVVGYG+D+AE+YRNLPY+G+L
Sbjct: 589 KAKSVKLVTLLDKPAGRNVEIE-----ADYVGFVVPNEFVVGYGLDYAERYRNLPYIGIL 646

Query: 182 KPECY 187
           KPE Y
Sbjct: 649 KPEIY 646

[Close]

BLAST of Moc04g32650 vs. ExPASy TrEMBL
Match: A0A6J1BZ71 (Hypoxanthine phosphoribosyltransferase OS=Momordica charantia OX=3673 GN=LOC111006034 PE=3 SV=1)

HSP 1 Score: 382.9 bits (982), Expect = 7.8e-103
Identity = 187/187 (100.00%), Postives = 187/187 (100.00%), Query Frame = 0


Query: 1   MAVHSKIETVLWNQDQISARVADIASRISLDFPAFSSPPVLVGVATGAFVFLADLVRHIN 60
           MAVHSKIETVLWNQDQISARVADIASRISLDFPAFSSPPVLVGVATGAFVFLADLVRHIN
Sbjct: 1   MAVHSKIETVLWNQDQISARVADIASRISLDFPAFSSPPVLVGVATGAFVFLADLVRHIN 60

Query: 61  LPISVDLVRVESYGFGTHSNGAPRISSDLKVDVKNKHVILVEDIVDTGNTLSCLIAHMEA 120
           LPISVDLVRVESYGFGTHSNGAPRISSDLKVDVKNKHVILVEDIVDTGNTLSCLIAHMEA
Sbjct: 61  LPISVDLVRVESYGFGTHSNGAPRISSDLKVDVKNKHVILVEDIVDTGNTLSCLIAHMEA 120

Query: 121 KGASSVSVCTFLDKPTRRKVHFQLVGEGKFYCGFECPDYFVVGYGMDFAEQYRNLPYVGV 180
           KGASSVSVCTFLDKPTRRKVHFQLVGEGKFYCGFECPDYFVVGYGMDFAEQYRNLPYVGV
Sbjct: 121 KGASSVSVCTFLDKPTRRKVHFQLVGEGKFYCGFECPDYFVVGYGMDFAEQYRNLPYVGV 180

Query: 181 LKPECYQ 188
           LKPECYQ
Sbjct: 181 LKPECYQ 187

[Close]

BLAST of Moc04g32650 vs. ExPASy TrEMBL
Match: A0A5A7SQ16 (Hypoxanthine phosphoribosyltransferase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold409G00870 PE=3 SV=1)

HSP 1 Score: 338.6 bits (867), Expect = 1.7e-89
Identity = 163/187 (87.17%), Postives = 174/187 (93.05%), Query Frame = 0


Query: 1   MAVHSKIETVLWNQDQISARVADIASRISLDFPAFSSPPVLVGVATGAFVFLADLVRHIN 60
           MAVHS IE VLW  DQISARV+D+AS+IS+ F   SSPPVLVGVATGAF+FLADLVRHIN
Sbjct: 1   MAVHSHIERVLWTHDQISARVSDLASQISIAFHGASSPPVLVGVATGAFIFLADLVRHIN 60

Query: 61  LPISVDLVRVESYGFGTHSNGAPRISSDLKVDVKNKHVILVEDIVDTGNTLSCLIAHMEA 120
           +PI+VD VRVESYGFGTHSNGAP ISSDLKVDVKNKHVILVEDIVDTGNTLSCLIAHME 
Sbjct: 61  IPIAVDFVRVESYGFGTHSNGAPTISSDLKVDVKNKHVILVEDIVDTGNTLSCLIAHMEV 120

Query: 121 KGASSVSVCTFLDKPTRRKVHFQLVGEGKFYCGFECPDYFVVGYGMDFAEQYRNLPYVGV 180
           KGASSVSVCTFLDKPTRR+V+FQLVGEGKFY GF+CPDYFVVGYGMDFAE YRNLPYVG+
Sbjct: 121 KGASSVSVCTFLDKPTRRQVNFQLVGEGKFYSGFQCPDYFVVGYGMDFAELYRNLPYVGI 180

Query: 181 LKPECYQ 188
           LKPECYQ
Sbjct: 181 LKPECYQ 187

[Close]

BLAST of Moc04g32650 vs. ExPASy TrEMBL
Match: A0A1S3C3Z8 (Hypoxanthine phosphoribosyltransferase OS=Cucumis melo OX=3656 GN=LOC103496579 PE=3 SV=1)

HSP 1 Score: 338.6 bits (867), Expect = 1.7e-89
Identity = 163/187 (87.17%), Postives = 174/187 (93.05%), Query Frame = 0


Query: 1   MAVHSKIETVLWNQDQISARVADIASRISLDFPAFSSPPVLVGVATGAFVFLADLVRHIN 60
           MAVHS IE VLW  DQISARV+D+AS+IS+ F   SSPPVLVGVATGAF+FLADLVRHIN
Sbjct: 20  MAVHSHIERVLWTHDQISARVSDLASQISIAFHGASSPPVLVGVATGAFIFLADLVRHIN 79

Query: 61  LPISVDLVRVESYGFGTHSNGAPRISSDLKVDVKNKHVILVEDIVDTGNTLSCLIAHMEA 120
           +PI+VD VRVESYGFGTHSNGAP ISSDLKVDVKNKHVILVEDIVDTGNTLSCLIAHME 
Sbjct: 80  IPIAVDFVRVESYGFGTHSNGAPTISSDLKVDVKNKHVILVEDIVDTGNTLSCLIAHMEV 139

Query: 121 KGASSVSVCTFLDKPTRRKVHFQLVGEGKFYCGFECPDYFVVGYGMDFAEQYRNLPYVGV 180
           KGASSVSVCTFLDKPTRR+V+FQLVGEGKFY GF+CPDYFVVGYGMDFAE YRNLPYVG+
Sbjct: 140 KGASSVSVCTFLDKPTRRQVNFQLVGEGKFYSGFQCPDYFVVGYGMDFAELYRNLPYVGI 199

Query: 181 LKPECYQ 188
           LKPECYQ
Sbjct: 200 LKPECYQ 206

[Close]

BLAST of Moc04g32650 vs. ExPASy TrEMBL
Match: A0A6J1GQL9 (Hypoxanthine phosphoribosyltransferase OS=Cucurbita moschata OX=3662 GN=LOC111456203 PE=3 SV=1)

HSP 1 Score: 337.8 bits (865), Expect = 2.9e-89
Identity = 164/187 (87.70%), Postives = 175/187 (93.58%), Query Frame = 0


Query: 1   MAVHSKIETVLWNQDQISARVADIASRISLDFPAFSSPPVLVGVATGAFVFLADLVRHIN 60
           MA+HS IE VLWNQDQISARVAD+AS+IS DF   SSPPV+VGVATGAF+FLADLVR IN
Sbjct: 1   MALHSHIERVLWNQDQISARVADLASQISADFSETSSPPVIVGVATGAFIFLADLVRLIN 60

Query: 61  LPISVDLVRVESYGFGTHSNGAPRISSDLKVDVKNKHVILVEDIVDTGNTLSCLIAHMEA 120
           LPI++D VRVESYG GT SNG+PRISSDLKVDVKNKHVILVEDIVD+GNTLSCL+AHMEA
Sbjct: 61  LPITLDSVRVESYGVGTESNGSPRISSDLKVDVKNKHVILVEDIVDSGNTLSCLLAHMEA 120

Query: 121 KGASSVSVCTFLDKPTRRKVHFQLVGEGKFYCGFECPDYFVVGYGMDFAEQYRNLPYVGV 180
           KGASSVSVCTFLDKPTRRKVHFQLVGEGKFY GFECPDYFVVGYGMDFAE YRNLPY+GV
Sbjct: 121 KGASSVSVCTFLDKPTRRKVHFQLVGEGKFYHGFECPDYFVVGYGMDFAELYRNLPYIGV 180

Query: 181 LKPECYQ 188
           LKPECYQ
Sbjct: 181 LKPECYQ 187

[Close]

BLAST of Moc04g32650 vs. ExPASy TrEMBL
Match: A0A6J1JQ71 (Hypoxanthine phosphoribosyltransferase OS=Cucurbita maxima OX=3661 GN=LOC111487323 PE=3 SV=1)

HSP 1 Score: 334.7 bits (857), Expect = 2.4e-88
Identity = 162/187 (86.63%), Postives = 173/187 (92.51%), Query Frame = 0


Query: 1   MAVHSKIETVLWNQDQISARVADIASRISLDFPAFSSPPVLVGVATGAFVFLADLVRHIN 60
           MA+HS IE VLWNQDQI ARVAD+AS+IS DF   SSPPV+VGVATGAF+FLADLVR IN
Sbjct: 1   MALHSHIERVLWNQDQILARVADLASQISADFSETSSPPVIVGVATGAFIFLADLVRRIN 60

Query: 61  LPISVDLVRVESYGFGTHSNGAPRISSDLKVDVKNKHVILVEDIVDTGNTLSCLIAHMEA 120
           LPI++D VRVESYG GT SNGAP ISSDLKVDVKNKHVILVEDIVD+GNTLSCL+AHMEA
Sbjct: 61  LPITLDFVRVESYGVGTESNGAPGISSDLKVDVKNKHVILVEDIVDSGNTLSCLLAHMEA 120

Query: 121 KGASSVSVCTFLDKPTRRKVHFQLVGEGKFYCGFECPDYFVVGYGMDFAEQYRNLPYVGV 180
           KGAS+VSVCTFLDKPTRRKVHFQLVGEGKFY GFECPDYFVVGYGMDFAE YRNLPY+GV
Sbjct: 121 KGASAVSVCTFLDKPTRRKVHFQLVGEGKFYHGFECPDYFVVGYGMDFAELYRNLPYIGV 180

Query: 181 LKPECYQ 188
           LKPECYQ
Sbjct: 181 LKPECYQ 187

[Close]

BLAST of Moc04g32650 vs. TAIR 10
Match: AT1G71750.1 (Hypoxanthine-guanine phosphoribosyltransferase )

HSP 1 Score: 258.8 bits (660), Expect = 3.3e-69
Identity = 127/187 (67.91%), Postives = 151/187 (80.75%), Query Frame = 0


Query: 1   MAVHSKIETVLWNQDQISARVADIASRISLDFPAFS-SPPVLVGVATGAFVFLADLVRHI 60
           MA+   IE VL++ + I+ RV  +   I+ DF   S   P+ VGVATGA +FLADLVR I
Sbjct: 1   MALEKHIEKVLFSDEVIAHRVNQLGIDITSDFSGDSEETPIFVGVATGACLFLADLVRRI 60

Query: 61  NLPISVDLVRVESYGFGTHSNGAPRISSDLKVDVKNKHVILVEDIVDTGNTLSCLIAHME 120
           +LPI++D +R ESYG GT S+G PR+S DLK+D+ NKHV+LVEDIVDTGNTLSCLI HM+
Sbjct: 61  DLPIAIDFIRAESYGSGTVSSGVPRVSFDLKLDITNKHVVLVEDIVDTGNTLSCLIEHMK 120

Query: 121 AKGASSVSVCTFLDKPTRRKVHFQLVGEGKFYCGFECPDYFVVGYGMDFAEQYRNLPYVG 180
           AK ASSVSVCT LDKP+RRKVH++LVG+GKFY GFECPD FVVGYGMDFAEQYRNL Y+G
Sbjct: 121 AKKASSVSVCTLLDKPSRRKVHYKLVGKGKFYSGFECPDEFVVGYGMDFAEQYRNLSYIG 180

Query: 181 VLKPECY 187
           VLKPE Y
Sbjct: 181 VLKPEYY 187

[Close]

BLAST of Moc04g32650 vs. TAIR 10
Match: AT1G71750.2 (Hypoxanthine-guanine phosphoribosyltransferase )

HSP 1 Score: 250.8 bits (639), Expect = 8.9e-67
Identity = 127/197 (64.47%), Postives = 151/197 (76.65%), Query Frame = 0


Query: 1   MAVHSKIETVLWNQDQISARVADIASRISLDFPAFS-SPPVLVGVATGAFVFLADLVRHI 60
           MA+   IE VL++ + I+ RV  +   I+ DF   S   P+ VGVATGA +FLADLVR I
Sbjct: 1   MALEKHIEKVLFSDEVIAHRVNQLGIDITSDFSGDSEETPIFVGVATGACLFLADLVRRI 60

Query: 61  NLPISVDLVRVESYGFGTHSNGAPRISSDLKVDVKNKHVILVEDIVDTGNTLSCLIAHME 120
           +LPI++D +R ESYG GT S+G PR+S DLK+D+ NKHV+LVEDIVDTGNTLSCLI HM+
Sbjct: 61  DLPIAIDFIRAESYGSGTVSSGVPRVSFDLKLDITNKHVVLVEDIVDTGNTLSCLIEHMK 120

Query: 121 AKGASSVSVCTFLDKPTRRKVHFQLVGEGKFYCGFE----------CPDYFVVGYGMDFA 180
           AK ASSVSVCT LDKP+RRKVH++LVG+GKFY GFE          CPD FVVGYGMDFA
Sbjct: 121 AKKASSVSVCTLLDKPSRRKVHYKLVGKGKFYSGFELNHGKQKYLLCPDEFVVGYGMDFA 180

Query: 181 EQYRNLPYVGVLKPECY 187
           EQYRNL Y+GVLKPE Y
Sbjct: 181 EQYRNLSYIGVLKPEYY 197

The following BLAST results are available for this feature:

BLAST of Moc04g32650 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022133463.1	1.6e-102	100.00	uncharacterized protein LOC111006034 [Momordica charantia]	[more]
XP_023527455.1	1.6e-89	87.70	uncharacterized protein LOC111790677 [Cucurbita pepo subsp. pepo]	[more]
KAA0031631.1	3.5e-89	87.17	hypoxanthine-guanine phosphoribosyltransferase [Cucumis melo var. makuwa] >TYK04...	[more]
XP_008456720.1	3.5e-89	87.17	PREDICTED: hypoxanthine-guanine phosphoribosyltransferase [Cucumis melo]	[more]
XP_022953780.1	5.9e-89	87.70	uncharacterized protein LOC111456203 [Cucurbita moschata]	[more]

BLAST of Moc04g32650 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q839B2	2.1e-36	41.62	Hypoxanthine-guanine phosphoribosyltransferase OS=Enterococcus faecalis (strain ...	[more]
P37472	1.8e-35	40.33	Hypoxanthine-guanine phosphoribosyltransferase OS=Bacillus subtilis (strain 168)...	[more]
Q92F56	3.0e-35	39.46	Bifunctional protein TilS/HprT OS=Listeria innocua serovar 6a (strain ATCC BAA-6...	[more]
Q724J4	3.0e-35	38.92	Bifunctional protein TilS/HprT OS=Listeria monocytogenes serotype 4b (strain F23...	[more]
Q8YAC7	3.0e-35	38.92	Bifunctional protein TilS/HprT OS=Listeria monocytogenes serovar 1/2a (strain AT...	[more]

BLAST of Moc04g32650 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1BZ71	7.8e-103	100.00	Hypoxanthine phosphoribosyltransferase OS=Momordica charantia OX=3673 GN=LOC1110...	[more]
A0A5A7SQ16	1.7e-89	87.17	Hypoxanthine phosphoribosyltransferase OS=Cucumis melo var. makuwa OX=1194695 GN...	[more]
A0A1S3C3Z8	1.7e-89	87.17	Hypoxanthine phosphoribosyltransferase OS=Cucumis melo OX=3656 GN=LOC103496579 P...	[more]
A0A6J1GQL9	2.9e-89	87.70	Hypoxanthine phosphoribosyltransferase OS=Cucurbita moschata OX=3662 GN=LOC11145...	[more]
A0A6J1JQ71	2.4e-88	86.63	Hypoxanthine phosphoribosyltransferase OS=Cucurbita maxima OX=3661 GN=LOC1114873...	[more]

BLAST of Moc04g32650 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G71750.1	3.3e-69	67.91	Hypoxanthine-guanine phosphoribosyltransferase	[more]
AT1G71750.2	8.9e-67	64.47	Hypoxanthine-guanine phosphoribosyltransferase	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (OHB3-1) v2
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR029057	Phosphoribosyltransferase-like	GENE3D	3.40.50.2020		coord: 1..187 e-value: 2.3E-62 score: 211.8
IPR029057	Phosphoribosyltransferase-like	SUPERFAMILY	53271	PRTase-like	coord: 3..186
IPR005904	Hypoxanthine phosphoribosyl transferase	TIGRFAM	TIGR01203	TIGR01203	coord: 10..182 e-value: 7.3E-51 score: 170.2
IPR000836	Phosphoribosyltransferase domain	PFAM	PF00156	Pribosyltran	coord: 14..168 e-value: 3.3E-25 score: 88.4
IPR000836	Phosphoribosyltransferase domain	CDD	cd06223	PRTases_typeI	coord: 38..155 e-value: 3.38572E-25 score: 92.844
None	No IPR available	PANTHER	PTHR43340:SF1	HYPOXANTHINE PHOSPHORIBOSYLTRANSFERASE	coord: 4..187
None	No IPR available	PANTHER	PTHR43340	HYPOXANTHINE-GUANINE PHOSPHORIBOSYLTRANSFERASE	coord: 4..187

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Moc04g32650.1	Moc04g32650.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0032263	GMP salvage
biological_process	GO:0006178	guanine salvage
biological_process	GO:0046100	hypoxanthine metabolic process
biological_process	GO:0032264	IMP salvage
biological_process	GO:0006166	purine ribonucleoside salvage
biological_process	GO:0009845	seed germination
biological_process	GO:0009116	nucleoside metabolic process
cellular_component	GO:0005737	cytoplasm
molecular_function	GO:0052657	guanine phosphoribosyltransferase activity
molecular_function	GO:0004422	hypoxanthine phosphoribosyltransferase activity
molecular_function	GO:0000287	magnesium ion binding
molecular_function	GO:0000166	nucleotide binding