Moc04g31620 (gene) Bitter gourd (OHB3-1) v2

Overview
NameMoc04g31620
Typegene
OrganismMomordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
DescriptionRetrovirus-related Pol polyprotein from transposon TNT 1-94
Locationchr4: 23771912 .. 23773153 (-)
RNA-Seq ExpressionMoc04g31620
SyntenyMoc04g31620
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTTTGTGCAACAGTCGATTGGTAATATGGAAACAAGCCAAACGAACATATCTGCACCATCGAGTTCCTCTATAGCAACAGAAGCAGCCGTCAATCCACTATATGAGTCTTGGGTAACTACCGACCAGCTACTTCTTGGTTGGTTGTACAACTCTATGACTCCAGAAGTTGCAACACAAGTGATGGGGTACGAAAATGCTTGTGATTTATGGGCTGCCATACAAGAACTCTTTGGAGTACAGTCTCAGGCGGAAGAAGATTATCTCCGTCAGGTATTTGAACAAACTCGAAAAGGTTCTCTTAAAATGACTGATTTTTTGCGTGTTATGAAGTCTCATGCAGACAATTTGGGTCAAGCTGGAAGCCCCGTACCCACTCGATCTTTGATTTCTCAAGTTTTGCTGGGATTAGATGAAGAGTATAATCCTGTGGTAGCAACGATCCAAGGAAAACGAGGCATTTCGTGGCCTGAAATGCAAGCCGAATTGTTGGTATTTGAGAAGAGGTTAGAACTTCAGAATTCTCATAAAAATACAGTATCTTTTAACAACTCTGTTTCTGTGAATATGGCTAATAGTAGCAGAAGTGTAAGTGGTGGAAACCAACGTCAAAATCAAAACTCTCGGCCACCATTCAACAACAATCGGTGGTCGAAATCGAGGTAGAGGACGGTGGAACAACAACAATAGTCGGCAAATTTGTCAGGTGTGTGGAAAACTTGGACATTCAGCACTAACGTGCTACCATCGATTTGATAAGGAGTACAAGAACAATACACAAAGCCAGGGTAAAAACTTCAATCGCGACTCTAACCAGGGGGTTAACAACAACTCTGGACAAGGTACATCTTATGCCTTCACAGCAACCCAAAATAACAATCCTTTTTTGGCCAATCCAGAAAAAGTGATAGACCCGAATTGGTATGTGGATAGTGGTGCTTCAAATCATGTCACCGCCGACTACAATAGTATGGTTCAACCTACTGAGTATGGAGGTATGGAAAGAGTTACAGTAGGTAATGGCGATAAATTAAAAATATCTCATGTTGGCAAATCCTGTTTAGTTTCTGACGGTGGGTTGGTCATGCTTGAAAATGTGTTGTGCGTATCTAACATAGCTAAAAATCTAGTTAGCGTGTCTAAACTCGCTAAAGACAATAACGTATACCTTGAATTTCATGCTGATTCTTGTCTTGTAAAGGATATACGTTCGGGCAAGGTGGTGCTGAAAGGGGCTCTTAA

mRNA sequence

ATGTTTGTGCAACAGTCGATTGGTAATATGGAAACAAGCCAAACGAACATATCTGCACCATCGAGTTCCTCTATAGCAACAGAAGCAGCCGTCAATCCACTATATGAGTCTTGGGTAACTACCGACCAGCTACTTCTTGGTTGGTTGTACAACTCTATGACTCCAGAAGTTGCAACACAAGTGATGGGGTACGAAAATGCTTGTGATTTATGGGCTGCCATACAAGAACTCTTTGGAGTACAGTCTCAGGCGGAAGAAGATTATCTCCGTCAGGTATTTGAACAAACTCGAAAAGGTTCTCTTAAAATGACTGATTTTTTGCGTGTTATGAAGTCTCATGCAGACAATTTGGGTCAAGCTGGAAGCCCCGTACCCACTCGATCTTTGATTTCTCAAGTTTTGCTGGGATTAGATGAAGAGTATAATCCTGTGGTAGCAACGATCCAAGGAAAACGAGGCATTTCGTGGCCTGAAATGCAAGCCGAATTGTTGGTATTTGAGAAGAGGTTAGAACTTCAGAATTCTCATAAAAATACAGTATCTTTTAACAACTCTGTTTCTGTGAATATGGCTAATAGTAGCAGAAGTGGTAAAAACTTCAATCGCGACTCTAACCAGGGGGTTAACAACAACTCTGGACAAGGTACATCTTATGCCTTCACAGCAACCCAAAATAACAATCCTTTTTTGGCCAATCCAGAAAAAGTGATAGACCCGAATTGGTATGTGGATAGTGGTGCTTCAAATCATGTCACCGCCGACTACAATAGTATGGTTCAACCTACTGAGTATGGAGGTATGGAAAGAGTTACAGTAGGATATACGTTCGGGCAAGGTGGTGCTGAAAGGGGCTCTTAA

Coding sequence (CDS)

ATGTTTGTGCAACAGTCGATTGGTAATATGGAAACAAGCCAAACGAACATATCTGCACCATCGAGTTCCTCTATAGCAACAGAAGCAGCCGTCAATCCACTATATGAGTCTTGGGTAACTACCGACCAGCTACTTCTTGGTTGGTTGTACAACTCTATGACTCCAGAAGTTGCAACACAAGTGATGGGGTACGAAAATGCTTGTGATTTATGGGCTGCCATACAAGAACTCTTTGGAGTACAGTCTCAGGCGGAAGAAGATTATCTCCGTCAGGTATTTGAACAAACTCGAAAAGGTTCTCTTAAAATGACTGATTTTTTGCGTGTTATGAAGTCTCATGCAGACAATTTGGGTCAAGCTGGAAGCCCCGTACCCACTCGATCTTTGATTTCTCAAGTTTTGCTGGGATTAGATGAAGAGTATAATCCTGTGGTAGCAACGATCCAAGGAAAACGAGGCATTTCGTGGCCTGAAATGCAAGCCGAATTGTTGGTATTTGAGAAGAGGTTAGAACTTCAGAATTCTCATAAAAATACAGTATCTTTTAACAACTCTGTTTCTGTGAATATGGCTAATAGTAGCAGAAGTGGTAAAAACTTCAATCGCGACTCTAACCAGGGGGTTAACAACAACTCTGGACAAGGTACATCTTATGCCTTCACAGCAACCCAAAATAACAATCCTTTTTTGGCCAATCCAGAAAAAGTGATAGACCCGAATTGGTATGTGGATAGTGGTGCTTCAAATCATGTCACCGCCGACTACAATAGTATGGTTCAACCTACTGAGTATGGAGGTATGGAAAGAGTTACAGTAGGATATACGTTCGGGCAAGGTGGTGCTGAAAGGGGCTCTTAA

Protein sequence

MFVQQSIGNMETSQTNISAPSSSSIATEAAVNPLYESWVTTDQLLLGWLYNSMTPEVATQVMGYENACDLWAAIQELFGVQSQAEEDYLRQVFEQTRKGSLKMTDFLRVMKSHADNLGQAGSPVPTRSLISQVLLGLDEEYNPVVATIQGKRGISWPEMQAELLVFEKRLELQNSHKNTVSFNNSVSVNMANSSRSGKNFNRDSNQGVNNNSGQGTSYAFTATQNNNPFLANPEKVIDPNWYVDSGASNHVTADYNSMVQPTEYGGMERVTVGYTFGQGGAERGS
Homology
BLAST of Moc04g31620 vs. NCBI nr
Match: XP_022148963.1 (uncharacterized protein LOC111017501 [Momordica charantia])

HSP 1 Score: 319.7 bits (818), Expect = 2.6e-83
Identity = 165/185 (89.19%), Postives = 170/185 (91.89%), Query Frame = 0

Query: 1   MFVQQSIGNMETSQTNISAPSSSSIATEAAVNPLYESWVTTDQLLLGWLYNSMTPEVATQ 60
           MFVQQSIGNMETSQTNISAPSSSSIATEAA+NPLYESWVTTDQLLLGWLYNSMTPEVATQ
Sbjct: 1   MFVQQSIGNMETSQTNISAPSSSSIATEAAINPLYESWVTTDQLLLGWLYNSMTPEVATQ 60

Query: 61  VMGYENACDLWAAIQELFGVQSQAEEDYLRQVFEQTRKGSLKMTDFLRVMKSHADNLGQA 120
           VMGYENACDLWAAIQELFGVQSQAEEDYLRQVF+QTRKGSLKMTDFLRVMKSHADNLGQA
Sbjct: 61  VMGYENACDLWAAIQELFGVQSQAEEDYLRQVFQQTRKGSLKMTDFLRVMKSHADNLGQA 120

Query: 121 GSPVPTRSLISQVLLGLDEEYNPVVATIQGKRGISWPEMQAELLVFEKRLELQNSHKNTV 180
           GSPVPTRSLISQVLLGLDEEYNPVVATIQGKRGISWPEMQAE        + QN +    
Sbjct: 121 GSPVPTRSLISQVLLGLDEEYNPVVATIQGKRGISWPEMQAEFRSVSGGNQRQNQNSQP- 180

Query: 181 SFNNS 186
            FNN+
Sbjct: 181 PFNNN 184

BLAST of Moc04g31620 vs. NCBI nr
Match: XP_038905164.1 (uncharacterized protein LOC120091275 isoform X4 [Benincasa hispida])

HSP 1 Score: 243.4 bits (620), Expect = 2.3e-60
Identity = 155/348 (44.54%), Postives = 191/348 (54.89%), Query Frame = 0

Query: 1   MFVQQSIGN-----------METSQTNISAPSSSSIATEAAVNPLYESWVTTDQLLLGWL 60
           MF+Q +IG               S    S   +SS  T   VNP YESW+  DQLLLGWL
Sbjct: 1   MFLQSAIGESIPIGSTGAGAAPRSIKGSSGSGASSSLTALEVNPQYESWMAVDQLLLGWL 60

Query: 61  YNSMTPEVATQVMGYENACDLWAAIQELFGVQSQAEEDYLRQVFEQTRKGSLKMTDFLRV 120
           YNSMTPEVA QVMG E A DLW +I +LFGVQS+ EEDYLR VF+ TRKG+LKM ++L+ 
Sbjct: 61  YNSMTPEVAIQVMGCECAKDLWTSIPQLFGVQSRVEEDYLRHVFQTTRKGNLKMEEYLQT 120

Query: 121 MKSHADNLGQAGSPVPTRSLISQVLLGLDEEYNPVVATIQGKRGISWPEMQAELLVFEKR 180
           MK + DNL QAGSP+P R+L+SQVLLGLDEEYN +VA IQG+  +SW +MQ+ELL++E+R
Sbjct: 121 MKMNTDNLEQAGSPMPPRTLVSQVLLGLDEEYNAIVAMIQGRVDMSWLDMQSELLLYERR 180

Query: 181 LELQNSHKNTVSFN--NSVSVNMANS-------------------SRSGKNFNRDSNQGV 240
           LE Q++ K TV FN  ++ SVNM N+                    R G    R   +G 
Sbjct: 181 LEHQSNQKTTVGFNQISNASVNMTNTRHVNQNNKTNSSNQSIGGGQRGGGGHGRGRGRGR 240

Query: 241 NN----------------------------NSGQGTSYAFTATQ---------------N 274
           NN                            NS Q     F   Q                
Sbjct: 241 NNKKPVCQVCGKVGHIAFYCFNRYSRDFVPNSPQNKVEPFPNNQTKNTQPHPTALAIAYG 300

BLAST of Moc04g31620 vs. NCBI nr
Match: XP_038905161.1 (uncharacterized protein LOC120091275 isoform X1 [Benincasa hispida])

HSP 1 Score: 241.9 bits (616), Expect = 6.8e-60
Identity = 153/341 (44.87%), Postives = 189/341 (55.43%), Query Frame = 0

Query: 1   MFVQQSIGN-----------METSQTNISAPSSSSIATEAAVNPLYESWVTTDQLLLGWL 60
           MF+Q +IG               S    S   +SS  T   VNP YESW+  DQLLLGWL
Sbjct: 1   MFLQSAIGESIPIGSTGAGAAPRSIKGSSGSGASSSLTALEVNPQYESWMAVDQLLLGWL 60

Query: 61  YNSMTPEVATQVMGYENACDLWAAIQELFGVQSQAEEDYLRQVFEQTRKGSLKMTDFLRV 120
           YNSMTPEVA QVMG E A DLW +I +LFGVQS+ EEDYLR VF+ TRKG+LKM ++L+ 
Sbjct: 61  YNSMTPEVAIQVMGCECAKDLWTSIPQLFGVQSRVEEDYLRHVFQTTRKGNLKMEEYLQT 120

Query: 121 MKSHADNLGQAGSPVPTRSLISQVLLGLDEEYNPVVATIQGKRGISWPEMQAELLVFEKR 180
           MK + DNL QAGSP+P R+L+SQVLLGLDEEYN +VA IQG+  +SW +MQ+ELL++E+R
Sbjct: 121 MKMNTDNLEQAGSPMPPRTLVSQVLLGLDEEYNAIVAMIQGRVDMSWLDMQSELLLYERR 180

Query: 181 LELQNSHKNTVSFN--NSVSVNMANS-------------------SRSGKNFNRDSNQGV 240
           LE Q++ K TV FN  ++ SVNM N+                    R G    R   +G 
Sbjct: 181 LEHQSNQKTTVGFNQISNASVNMTNTRHVNQNNKTNSSNQSIGGGQRGGGGHGRGRGRGR 240

Query: 241 NN----------------------------NSGQGTSYAFTATQ---------------N 267
           NN                            NS Q     F   Q                
Sbjct: 241 NNKKPVCQVCGKVGHIAFYCFNRYSRDFVPNSPQNKVEPFPNNQTKNTQPHPTALAIAYG 300

BLAST of Moc04g31620 vs. NCBI nr
Match: XP_022151683.1 (uncharacterized protein LOC111019598 [Momordica charantia])

HSP 1 Score: 240.7 bits (613), Expect = 1.5e-59
Identity = 140/294 (47.62%), Postives = 185/294 (62.93%), Query Frame = 0

Query: 15  TNISAPSSSSIATEAAVNPLYESWVTTDQLLLGWLYNSMTPEVATQVMGYENACDLWAAI 74
           TNI   +SS   +   +NP YE+W+  D+LLLGWLYNSM  +VA QVMG+  + +LW A+
Sbjct: 82  TNIEGSTSSQ--SSPTLNPTYEAWIVVDKLLLGWLYNSMAADVAMQVMGFSTSRELWTAV 141

Query: 75  QELFGVQSQAEEDYLRQVFEQTRKGSLKMTDFLRVMKSHADNLGQAGSPVPTRSLISQVL 134
           QELFGVQS+AE DYL+QVF+QT KGSL+M ++L++MKSHADNL  AGS V  R L+SQVL
Sbjct: 142 QELFGVQSRAEVDYLKQVFQQTCKGSLQMIEYLKLMKSHADNLALAGSSVSVRDLVSQVL 201

Query: 135 LGLDEEYNPVVATIQGKRGISWPEMQAELLVFEKRLELQNSHKNTVSFN--NSVSVNMAN 194
            GLDEEYNP+V  +QGK  +SW EM AELL +EKRLE QNS K+ +  N   + SVN  +
Sbjct: 202 TGLDEEYNPIVVAVQGKVNLSWSEMHAELLTYEKRLEYQNSLKSGIPINQTQTPSVNYVD 261

Query: 195 SSRSGKNF--NRDSNQGVN---NNSGQGTSYA--------------------FT------ 254
               G++F  N+ +N G N   +N+ +G  Y                     FT      
Sbjct: 262 ----GRSFQTNQRTNNGNNSHGSNTHRGGGYQRGSFGQRNRGRGPQPTQHKNFTPSNSGP 321

Query: 255 ---ATQNNNPFLANPEKVIDPNWYVDSGASNHVTADYNSMVQPTEYGGMERVTV 273
              A  + +  +  PE VIDP+WY DSGA++HVTA+ N++ Q  +Y G E V V
Sbjct: 322 NVFAAHHTSTTVTTPETVIDPSWYADSGATSHVTANPNNVEQKVDYSGTENVIV 369

BLAST of Moc04g31620 vs. NCBI nr
Match: KAA0026100.1 (uncharacterized protein E6C27_scaffold19G00360 [Cucumis melo var. makuwa])

HSP 1 Score: 239.2 bits (609), Expect = 4.4e-59
Identity = 142/301 (47.18%), Postives = 179/301 (59.47%), Query Frame = 0

Query: 22  SSSIATEAAVNPLYESWVTTDQLLLGWLYNSMTPEVATQVMGYENACDLWAAIQELFGVQ 81
           +SS  T   VN L+E WVTTD LLLGWLYNSMTP+VA Q+MG+ N  DLW A Q+ FGVQ
Sbjct: 92  ASSSITPRIVNSLFEQWVTTDLLLLGWLYNSMTPDVAIQLMGFTNVEDLWDATQDFFGVQ 151

Query: 82  SQAEEDYLRQVFEQTRKGSLKMTDFLRVMKSHADNLGQAGSPVPTRSLISQVLLGLDEEY 141
           S+AEED+LRQ+ + TRKG+ KM ++L VMK++ DNLGQ GSPVP R+LISQVLLGLDE Y
Sbjct: 152 SRAEEDFLRQMLQTTRKGNTKMEEYLLVMKTNVDNLGQVGSPVPRRALISQVLLGLDEVY 211

Query: 142 NPVVATIQGKRGISWPEMQAELLVFEKRLELQNSHKNTVSFNN---SVSVNMA-----NS 201
           N V+  IQGK  ISW +MQ++LL+FEK L+ QN+ K      N   S ++NMA     N 
Sbjct: 212 NLVIVVIQGKPDISWLDMQSKLLIFEKILKHQNTQKKKKKKGNITQSPALNMAQRFALNG 271

Query: 202 SRSGKN----------------------------------------FNR--------DSN 261
            R+  N                                        FN+        D N
Sbjct: 272 QRNHSNKKFYGYNRQHFSGQRGNLNNGPTCQLCGKYGHSALVCYNRFNKEFSSPLVQDRN 331

Query: 262 QGVNNNSGQGTSYAFTATQNNNPFLANPEKVIDPNWYVDSGASNHVTADYNSMVQPTEYG 267
           +  +N S       F +TQN  PF A P+ V+DPNWY+DSGA+NHVT + ++M  PTEY 
Sbjct: 332 EHSSNGSVSPNPAVFVSTQNATPF-ATPDTVVDPNWYIDSGATNHVTRECSNMTNPTEYS 391

BLAST of Moc04g31620 vs. ExPASy Swiss-Prot
Match: Q94HW2 (Retrovirus-related Pol polyprotein from transposon RE1 OS=Arabidopsis thaliana OX=3702 GN=RE1 PE=2 SV=1)

HSP 1 Score: 77.0 bits (188), Expect = 3.8e-13
Identity = 76/303 (25.08%), Postives = 130/303 (42.90%), Query Frame = 0

Query: 23  SSIATEAA--VNPLYESWVTTDQLLLGWLYNSMTPEVATQVMGYENACDLWAAIQELFGV 82
           ++I T+AA  VNP Y  W   D+L+   +  +++  V   V     A  +W  +++++  
Sbjct: 60  ATIGTDAAPRVNPDYTRWKRQDKLIYSAVLGAISMSVQPAVSRATTAAQIWETLRKIYAN 119

Query: 83  QSQAEEDYLRQVFEQTRKGSLKMTDFLRVMKSHADNLGQAGSPVPTRSLISQVLLGLDEE 142
            S      LR   +Q  KG+  + D+++ + +  D L   G P+     + +VL  L EE
Sbjct: 120 PSYGHVTQLRTQLKQWTKGTKTIDDYMQGLVTRFDQLALLGKPMDHDEQVERVLENLPEE 179

Query: 143 YNPVVATIQGK-RGISWPEMQAELLVFEKRLELQN------------SHKNTVSFNNSVS 202
           Y PV+  I  K    +  E+   LL  E ++   +            SH+NT + NN+ +
Sbjct: 180 YKPVIDQIAAKDTPPTLTEIHERLLNHESKILAVSSATVIPITANAVSHRNTTTTNNNNN 239

Query: 203 VNMANS-------------SRSGKNFNRDSNQ-----------GVNNNSGQGTSY----- 262
            N  N               +S  NF+ ++NQ           GV  +S +  S      
Sbjct: 240 GNRNNRYDNRNNNNNSKPWQQSSTNFHPNNNQSKPYLGKCQICGVQGHSAKRCSQLQHFL 299

Query: 263 -AFTATQNNNPF--------LANPEKVIDPNWYVDSGASNHVTADYNSMVQPTEYGGMER 273
            +  + Q  +PF        LA        NW +DSGA++H+T+D+N++     Y G + 
Sbjct: 300 SSVNSQQPPSPFTPWQPRANLALGSPYSSNNWLLDSGATHHITSDFNNLSLHQPYTGGDD 359

BLAST of Moc04g31620 vs. ExPASy Swiss-Prot
Match: Q9ZT94 (Retrovirus-related Pol polyprotein from transposon RE2 OS=Arabidopsis thaliana OX=3702 GN=RE2 PE=4 SV=1)

HSP 1 Score: 52.0 bits (123), Expect = 1.3e-05
Identity = 67/310 (21.61%), Postives = 115/310 (37.10%), Query Frame = 0

Query: 23  SSIATEAA--VNPLYESWVTTDQLLLGWLYNSMTPEVATQVMGYENACDLWAAIQELFGV 82
           ++I T+A   VNP Y  W   D+L+   +  +++  V   V     A  +W  +++++  
Sbjct: 60  ATIGTDAVPRVNPDYTRWRRQDKLIYSAILGAISMSVQPAVSRATTAAQIWETLRKIYAN 119

Query: 83  QSQAEEDYLRQVFEQTRKGSLKMTDFLRVMKSHADNLGQAGSPVPTRSLISQVLLGLDEE 142
            S      LR +                   +  D L   G P+     + +VL  L ++
Sbjct: 120 PSYGHVTQLRFI-------------------TRFDQLALLGKPMDHDEQVERVLENLPDD 179

Query: 143 YNPVVATIQGK-RGISWPEMQAELLVFEKRLELQNSHKNTVSFNNSVSVNMANSSRSGKN 202
           Y PV+  I  K    S  E+   L+  E +L   NS +      N V+    N++R+   
Sbjct: 180 YKPVIDQIAAKDTPPSLTEIHERLINRESKLLALNSAEVVPITANVVTHRNTNTNRNQN- 239

Query: 203 FNRDSNQGVNNNSGQGTS--------------------------------------YAFT 262
            NR  N+  NNN+ +  S                                      + F 
Sbjct: 240 -NRGDNRNYNNNNNRSNSWQPSSSGSRSDNRQPKPYLGRCQICSVQGHSAKRCPQLHQFQ 299

Query: 263 ATQN-------------------NNPFLANPEKVIDPNWYVDSGASNHVTADYNSMVQPT 273
           +T N                   N+P+ AN       NW +DSGA++H+T+D+N++    
Sbjct: 300 STTNQQQSTSPFTPWQPRANLAVNSPYNAN-------NWLLDSGATHHITSDFNNLSFHQ 341

BLAST of Moc04g31620 vs. ExPASy TrEMBL
Match: A0A6J1D5J0 (uncharacterized protein LOC111017501 OS=Momordica charantia OX=3673 GN=LOC111017501 PE=4 SV=1)

HSP 1 Score: 319.7 bits (818), Expect = 1.2e-83
Identity = 165/185 (89.19%), Postives = 170/185 (91.89%), Query Frame = 0

Query: 1   MFVQQSIGNMETSQTNISAPSSSSIATEAAVNPLYESWVTTDQLLLGWLYNSMTPEVATQ 60
           MFVQQSIGNMETSQTNISAPSSSSIATEAA+NPLYESWVTTDQLLLGWLYNSMTPEVATQ
Sbjct: 1   MFVQQSIGNMETSQTNISAPSSSSIATEAAINPLYESWVTTDQLLLGWLYNSMTPEVATQ 60

Query: 61  VMGYENACDLWAAIQELFGVQSQAEEDYLRQVFEQTRKGSLKMTDFLRVMKSHADNLGQA 120
           VMGYENACDLWAAIQELFGVQSQAEEDYLRQVF+QTRKGSLKMTDFLRVMKSHADNLGQA
Sbjct: 61  VMGYENACDLWAAIQELFGVQSQAEEDYLRQVFQQTRKGSLKMTDFLRVMKSHADNLGQA 120

Query: 121 GSPVPTRSLISQVLLGLDEEYNPVVATIQGKRGISWPEMQAELLVFEKRLELQNSHKNTV 180
           GSPVPTRSLISQVLLGLDEEYNPVVATIQGKRGISWPEMQAE        + QN +    
Sbjct: 121 GSPVPTRSLISQVLLGLDEEYNPVVATIQGKRGISWPEMQAEFRSVSGGNQRQNQNSQP- 180

Query: 181 SFNNS 186
            FNN+
Sbjct: 181 PFNNN 184

BLAST of Moc04g31620 vs. ExPASy TrEMBL
Match: A0A6J1DCW4 (uncharacterized protein LOC111019598 OS=Momordica charantia OX=3673 GN=LOC111019598 PE=4 SV=1)

HSP 1 Score: 240.7 bits (613), Expect = 7.3e-60
Identity = 140/294 (47.62%), Postives = 185/294 (62.93%), Query Frame = 0

Query: 15  TNISAPSSSSIATEAAVNPLYESWVTTDQLLLGWLYNSMTPEVATQVMGYENACDLWAAI 74
           TNI   +SS   +   +NP YE+W+  D+LLLGWLYNSM  +VA QVMG+  + +LW A+
Sbjct: 82  TNIEGSTSSQ--SSPTLNPTYEAWIVVDKLLLGWLYNSMAADVAMQVMGFSTSRELWTAV 141

Query: 75  QELFGVQSQAEEDYLRQVFEQTRKGSLKMTDFLRVMKSHADNLGQAGSPVPTRSLISQVL 134
           QELFGVQS+AE DYL+QVF+QT KGSL+M ++L++MKSHADNL  AGS V  R L+SQVL
Sbjct: 142 QELFGVQSRAEVDYLKQVFQQTCKGSLQMIEYLKLMKSHADNLALAGSSVSVRDLVSQVL 201

Query: 135 LGLDEEYNPVVATIQGKRGISWPEMQAELLVFEKRLELQNSHKNTVSFN--NSVSVNMAN 194
            GLDEEYNP+V  +QGK  +SW EM AELL +EKRLE QNS K+ +  N   + SVN  +
Sbjct: 202 TGLDEEYNPIVVAVQGKVNLSWSEMHAELLTYEKRLEYQNSLKSGIPINQTQTPSVNYVD 261

Query: 195 SSRSGKNF--NRDSNQGVN---NNSGQGTSYA--------------------FT------ 254
               G++F  N+ +N G N   +N+ +G  Y                     FT      
Sbjct: 262 ----GRSFQTNQRTNNGNNSHGSNTHRGGGYQRGSFGQRNRGRGPQPTQHKNFTPSNSGP 321

Query: 255 ---ATQNNNPFLANPEKVIDPNWYVDSGASNHVTADYNSMVQPTEYGGMERVTV 273
              A  + +  +  PE VIDP+WY DSGA++HVTA+ N++ Q  +Y G E V V
Sbjct: 322 NVFAAHHTSTTVTTPETVIDPSWYADSGATSHVTANPNNVEQKVDYSGTENVIV 369

BLAST of Moc04g31620 vs. ExPASy TrEMBL
Match: A0A5A7SIT7 (Uncharacterized protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold19G00360 PE=4 SV=1)

HSP 1 Score: 239.2 bits (609), Expect = 2.1e-59
Identity = 142/301 (47.18%), Postives = 179/301 (59.47%), Query Frame = 0

Query: 22  SSSIATEAAVNPLYESWVTTDQLLLGWLYNSMTPEVATQVMGYENACDLWAAIQELFGVQ 81
           +SS  T   VN L+E WVTTD LLLGWLYNSMTP+VA Q+MG+ N  DLW A Q+ FGVQ
Sbjct: 92  ASSSITPRIVNSLFEQWVTTDLLLLGWLYNSMTPDVAIQLMGFTNVEDLWDATQDFFGVQ 151

Query: 82  SQAEEDYLRQVFEQTRKGSLKMTDFLRVMKSHADNLGQAGSPVPTRSLISQVLLGLDEEY 141
           S+AEED+LRQ+ + TRKG+ KM ++L VMK++ DNLGQ GSPVP R+LISQVLLGLDE Y
Sbjct: 152 SRAEEDFLRQMLQTTRKGNTKMEEYLLVMKTNVDNLGQVGSPVPRRALISQVLLGLDEVY 211

Query: 142 NPVVATIQGKRGISWPEMQAELLVFEKRLELQNSHKNTVSFNN---SVSVNMA-----NS 201
           N V+  IQGK  ISW +MQ++LL+FEK L+ QN+ K      N   S ++NMA     N 
Sbjct: 212 NLVIVVIQGKPDISWLDMQSKLLIFEKILKHQNTQKKKKKKGNITQSPALNMAQRFALNG 271

Query: 202 SRSGKN----------------------------------------FNR--------DSN 261
            R+  N                                        FN+        D N
Sbjct: 272 QRNHSNKKFYGYNRQHFSGQRGNLNNGPTCQLCGKYGHSALVCYNRFNKEFSSPLVQDRN 331

Query: 262 QGVNNNSGQGTSYAFTATQNNNPFLANPEKVIDPNWYVDSGASNHVTADYNSMVQPTEYG 267
           +  +N S       F +TQN  PF A P+ V+DPNWY+DSGA+NHVT + ++M  PTEY 
Sbjct: 332 EHSSNGSVSPNPAVFVSTQNATPF-ATPDTVVDPNWYIDSGATNHVTRECSNMTNPTEYS 391

BLAST of Moc04g31620 vs. ExPASy TrEMBL
Match: A0A5D3E3L7 (Uncharacterized protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold216G001590 PE=4 SV=1)

HSP 1 Score: 232.3 bits (591), Expect = 2.6e-57
Identity = 133/253 (52.57%), Postives = 169/253 (66.80%), Query Frame = 0

Query: 21  SSSSIATEAAVNPLYESWVTTDQLLLGWLYNSMTPEVATQVMGYENACDLWAAIQELFGV 80
           SSS+      VNP YE WVT+D LLLG +YNSM P+VA Q+MG+  A DLW AIQ LFG+
Sbjct: 55  SSSTSMNSKIVNPKYEQWVTSDMLLLGLIYNSMVPDVALQLMGFNTAKDLWEAIQNLFGI 114

Query: 81  QSQAEEDYLRQVFEQTRKGSLKMTDFLRVMKSHADNLGQAGSPVPTRSLISQVLLGLDEE 140
           +S+AEE +LR  F+ TR+G+ KM D+LR+MK +ADNLGQAGSPVP R LISQVLLGLDE 
Sbjct: 115 KSRAEEYFLRHTFQTTREGNYKMEDYLRIMKINADNLGQAGSPVPHRYLISQVLLGLDEV 174

Query: 141 YNPVVATIQGKRGISWPEMQAELLVFEKRLELQNSHKNTVSFNNSVSVNMANSSRSGKNF 200
           YNPV A IQGK  ISW +MQ+ELL+FE  +E+       +    S ++ MA      +N 
Sbjct: 175 YNPVTAVIQGKPDISWLDMQSELLIFENLVEI------VLIKMESETILMAADVVEEENR 234

Query: 201 NRDSNQGVNNNSGQGTSYAFTATQNNNPFLANPEKVIDPNWYVDSGASNHVTADYNSMVQ 260
             + NQ    N  Q    AF  TQ ++  LA PE V+D N YVDSGA+NHVT+D++++  
Sbjct: 235 GFNPNQ----NGKQIPDDAFITTQKSSS-LATPETVVDTNRYVDSGATNHVTSDHSNLWN 294

Query: 261 PTEYGGMERVTVG 274
             +Y G E V VG
Sbjct: 295 IDDYSGNENVVVG 296

BLAST of Moc04g31620 vs. ExPASy TrEMBL
Match: A0A5D3C373 (Retrovirus-related Pol polyprotein from transposon TNT 1-94 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold98G002430 PE=4 SV=1)

HSP 1 Score: 192.6 bits (488), Expect = 2.3e-45
Identity = 118/280 (42.14%), Postives = 159/280 (56.79%), Query Frame = 0

Query: 57  VATQVMGYENACDLWAAIQELFGVQSQAEEDYLRQVFEQTRKGSLKMTDFLRVMKSHADN 116
           +A Q+MG+ NA DLW A Q+LFGVQS+AEED+LRQ+F+ TRK      D+LR+MK+++D 
Sbjct: 45  IAIQLMGFTNAKDLWEATQDLFGVQSRAEEDFLRQMFQTTRKVRASYEDYLRIMKTNSDK 104

Query: 117 LGQAGSPVPTRSLISQVLLGLDEEYNPVVATIQGKRGISWPEMQAELLVFEKRLELQNSH 176
           LGQAGSPVP R+ ISQ LLGLDE YNPV+A IQGK  ISW +MQ+ELL FEKRLE Q++ 
Sbjct: 105 LGQAGSPVPKRAFISQALLGLDEVYNPVIAVIQGKPEISWIDMQSELLTFEKRLEHQDTQ 164

Query: 177 KNTVSFNNSVSVNMANSSRSGKNFNRDSN---QGVNNNSGQGTSYAFTATQN-------- 236
           KNT +   +V VN+A  +R+  +F + SN    G N N+ QG    F   +         
Sbjct: 165 KNTENIIQNV-VNIA-QNRNSSDFRKYSNHQFHGNNRNNSQGQRGGFNIGRGRGKGRGNK 224

Query: 237 --------------------NNPFL--------------------------------ANP 274
                               N  FL                                A  
Sbjct: 225 PTCQVCEKYGHSALVCYNRFNKEFLSPLVQDRGAQSSNFSKHSNLTVLVTGQSVNQFATA 284

BLAST of Moc04g31620 vs. TAIR 10
Match: AT1G34070.1 (CONTAINS InterPro DOMAIN/s: Retrotransposon gag protein (InterPro:IPR005162); BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT5G48050.1); Has 648 Blast hits to 647 proteins in 29 species: Archae - 0; Bacteria - 0; Metazoa - 16; Fungi - 25; Plants - 607; Viruses - 0; Other Eukaryotes - 0 (source: NCBI BLink). )

HSP 1 Score: 43.9 bits (102), Expect = 2.5e-04
Identity = 56/234 (23.93%), Postives = 97/234 (41.45%), Query Frame = 0

Query: 37  SWVTTDQLLLGWLYNSMTP-EVATQVMGYENACDLWAAIQELFGVQSQAEEDYLRQVFEQ 96
           +W   D ++   LY ++TP +     +    + D+W  I+  F     A    L      
Sbjct: 64  NWQKRDGIVKLSLYGTLTPKQFQGSFVTSSTSRDIWLRIKNQFRNNKDARALRLDSELRT 123

Query: 97  TRKGSLKMTDFLRVMKSHADNLGQAGSPVPTRSLISQVLLGLDEEYNPVVATIQGKRGI- 156
              G +++ D+ R MK  AD+L     PV  R+L+  VL GL+ +++ ++  I+ ++   
Sbjct: 124 KDIGDMRVADYYRKMKKLADSLRNVDVPVTDRNLVMYVLNGLNPKFDNIINVIKHRQPFP 183

Query: 157 SWPEMQAELLVFEKRLELQNSHKNTVSFNNSVSVNMA--------NSSRSGKNFNRDSNQ 216
           S+ +    L   E RL+       T   ++S S  +A        N  RSG N      +
Sbjct: 184 SFDDAATMLQEEEDRLKRAIKPNPTHVDHSSSSTVLACSEAPPVTNFQRSGGNQMGYRGR 243

Query: 217 GVNNNSGQGTSYAFT-------ATQNNNPFLANPEKVIDPNW----YVDSGASN 250
           G  NN  +G    F+        + N  PF  N  ++ +  W    YV++   N
Sbjct: 244 GRGNNIFRGRGGRFSYYNMPTFNSWNRPPFYQNSYQMWNHPWGYPPYVNTNGGN 297

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022148963.12.6e-8389.19uncharacterized protein LOC111017501 [Momordica charantia][more]
XP_038905164.12.3e-6044.54uncharacterized protein LOC120091275 isoform X4 [Benincasa hispida][more]
XP_038905161.16.8e-6044.87uncharacterized protein LOC120091275 isoform X1 [Benincasa hispida][more]
XP_022151683.11.5e-5947.62uncharacterized protein LOC111019598 [Momordica charantia][more]
KAA0026100.14.4e-5947.18uncharacterized protein E6C27_scaffold19G00360 [Cucumis melo var. makuwa][more]
Match NameE-valueIdentityDescription
Q94HW23.8e-1325.08Retrovirus-related Pol polyprotein from transposon RE1 OS=Arabidopsis thaliana O... [more]
Q9ZT941.3e-0521.61Retrovirus-related Pol polyprotein from transposon RE2 OS=Arabidopsis thaliana O... [more]
Match NameE-valueIdentityDescription
A0A6J1D5J01.2e-8389.19uncharacterized protein LOC111017501 OS=Momordica charantia OX=3673 GN=LOC111017... [more]
A0A6J1DCW47.3e-6047.62uncharacterized protein LOC111019598 OS=Momordica charantia OX=3673 GN=LOC111019... [more]
A0A5A7SIT72.1e-5947.18Uncharacterized protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold... [more]
A0A5D3E3L72.6e-5752.57Uncharacterized protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold... [more]
A0A5D3C3732.3e-4542.14Retrovirus-related Pol polyprotein from transposon TNT 1-94 OS=Cucumis melo var.... [more]
Match NameE-valueIdentityDescription
AT1G34070.12.5e-0423.93CONTAINS InterPro DOMAIN/s: Retrotransposon gag protein (InterPro:IPR005162); BE... [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (OHB3-1) v2
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availablePFAMPF14223Retrotran_gag_2coord: 38..170
e-value: 7.1E-12
score: 45.3
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..22
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 193..220
NoneNo IPR availablePANTHERPTHR34222FAMILY NOT NAMEDcoord: 27..272
NoneNo IPR availablePANTHERPTHR34222:SF48SUBFAMILY NOT NAMEDcoord: 27..272

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Moc04g31620.1Moc04g31620.1mRNA