Moc04g31580 (gene) Bitter gourd (OHB3-1) v2

Overview
NameMoc04g31580
Typegene
OrganismMomordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
DescriptionCalcium-binding EF-hand family protein
Locationchr4: 23751499 .. 23751999 (+)
RNA-Seq ExpressionMoc04g31580
SyntenyMoc04g31580
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTCTCCGATCTGCTCCAACGACTTGCTTAAGATCTTCAACAAACTCGACAAGAACTGCGATGGCCTCGTCTGCCTACAGGAGCTCCATTGGCTTCTCGACCGTATTGGCATCCAGCTAACCATGGAAGAGCTCGAGTCATCTCTGGAGAAACCAAGCCTCGACTTCAATGAGTTCTTGTTCTTTTACGAGTCGATATCGAAGCGAAACAAAGGCGAGTGCAACGGCGGTGTGGGCGATGACGAGAATGAGATGTTGGATGGGCATGAAGGGGAGGAAGATGTTGACATTGTCTATATGGCGTTCAAAGTTTTTGACATGAACGATGATGGCTTCATTTCTTGTGAGGAGCTTCGGAGTGTGTTGTTGAGACTGGAGCTTTGGGATTCAAACAGAAGCGACAGCGAGTGCCGAAACATGATCCGAGCTTATGACACCAATTTGGATGGGAAACTCGATTTCGAGGAGTTTAAGAACATGATGTTGCTCACGACTTCTTGA

mRNA sequence

ATGTCTCCGATCTGCTCCAACGACTTGCTTAAGATCTTCAACAAACTCGACAAGAACTGCGATGGCCTCGTCTGCCTACAGGAGCTCCATTGGCTTCTCGACCGTATTGGCATCCAGCTAACCATGGAAGAGCTCGAGTCATCTCTGGAGAAACCAAGCCTCGACTTCAATGAGTTCTTGTTCTTTTACGAGTCGATATCGAAGCGAAACAAAGGCGAGTGCAACGGCGGTGTGGGCGATGACGAGAATGAGATGTTGGATGGGCATGAAGGGGAGGAAGATGTTGACATTGTCTATATGGCGTTCAAAGTTTTTGACATGAACGATGATGGCTTCATTTCTTGTGAGGAGCTTCGGAGTGTGTTGTTGAGACTGGAGCTTTGGGATTCAAACAGAAGCGACAGCGAGTGCCGAAACATGATCCGAGCTTATGACACCAATTTGGATGGGAAACTCGATTTCGAGGAGTTTAAGAACATGATGTTGCTCACGACTTCTTGA

Coding sequence (CDS)

ATGTCTCCGATCTGCTCCAACGACTTGCTTAAGATCTTCAACAAACTCGACAAGAACTGCGATGGCCTCGTCTGCCTACAGGAGCTCCATTGGCTTCTCGACCGTATTGGCATCCAGCTAACCATGGAAGAGCTCGAGTCATCTCTGGAGAAACCAAGCCTCGACTTCAATGAGTTCTTGTTCTTTTACGAGTCGATATCGAAGCGAAACAAAGGCGAGTGCAACGGCGGTGTGGGCGATGACGAGAATGAGATGTTGGATGGGCATGAAGGGGAGGAAGATGTTGACATTGTCTATATGGCGTTCAAAGTTTTTGACATGAACGATGATGGCTTCATTTCTTGTGAGGAGCTTCGGAGTGTGTTGTTGAGACTGGAGCTTTGGGATTCAAACAGAAGCGACAGCGAGTGCCGAAACATGATCCGAGCTTATGACACCAATTTGGATGGGAAACTCGATTTCGAGGAGTTTAAGAACATGATGTTGCTCACGACTTCTTGA

Protein sequence

MSPICSNDLLKIFNKLDKNCDGLVCLQELHWLLDRIGIQLTMEELESSLEKPSLDFNEFLFFYESISKRNKGECNGGVGDDENEMLDGHEGEEDVDIVYMAFKVFDMNDDGFISCEELRSVLLRLELWDSNRSDSECRNMIRAYDTNLDGKLDFEEFKNMMLLTTS
Homology
BLAST of Moc04g31580 vs. NCBI nr
Match: XP_022141411.1 (probable calcium-binding protein CML44 [Momordica charantia])

HSP 1 Score: 340.1 bits (871), Expect = 1.1e-89
Identity = 166/166 (100.00%), Postives = 166/166 (100.00%), Query Frame = 0

Query: 1   MSPICSNDLLKIFNKLDKNCDGLVCLQELHWLLDRIGIQLTMEELESSLEKPSLDFNEFL 60
           MSPICSNDLLKIFNKLDKNCDGLVCLQELHWLLDRIGIQLTMEELESSLEKPSLDFNEFL
Sbjct: 1   MSPICSNDLLKIFNKLDKNCDGLVCLQELHWLLDRIGIQLTMEELESSLEKPSLDFNEFL 60

Query: 61  FFYESISKRNKGECNGGVGDDENEMLDGHEGEEDVDIVYMAFKVFDMNDDGFISCEELRS 120
           FFYESISKRNKGECNGGVGDDENEMLDGHEGEEDVDIVYMAFKVFDMNDDGFISCEELRS
Sbjct: 61  FFYESISKRNKGECNGGVGDDENEMLDGHEGEEDVDIVYMAFKVFDMNDDGFISCEELRS 120

Query: 121 VLLRLELWDSNRSDSECRNMIRAYDTNLDGKLDFEEFKNMMLLTTS 167
           VLLRLELWDSNRSDSECRNMIRAYDTNLDGKLDFEEFKNMMLLTTS
Sbjct: 121 VLLRLELWDSNRSDSECRNMIRAYDTNLDGKLDFEEFKNMMLLTTS 166

BLAST of Moc04g31580 vs. NCBI nr
Match: XP_038885742.1 (probable calcium-binding protein CML44 [Benincasa hispida])

HSP 1 Score: 251.9 bits (642), Expect = 3.8e-63
Identity = 130/166 (78.31%), Postives = 143/166 (86.14%), Query Frame = 0

Query: 1   MSPICSNDLLKIFNKLDKNCDGLVCLQELHWLLDRIGIQLTMEELESSLEKPSLDFNEFL 60
           MS I  NDL +IFNKLDKN DGL+CLQEL WL DRIGIQLTMEELES LEKPSLDFNEFL
Sbjct: 11  MSSISFNDLHRIFNKLDKNGDGLICLQELKWLFDRIGIQLTMEELESFLEKPSLDFNEFL 70

Query: 61  FFYESISKRNKGECNGGVGDDENEMLDGHEGEEDVDIVYMAFKVFDMNDDGFISCEELRS 120
           FFYESISK+NKGEC GGVG     + D H  EE+++IVYMAFKVFDMN DGFISC+EL +
Sbjct: 71  FFYESISKQNKGECKGGVGC----LQDDH--EEEMEIVYMAFKVFDMNGDGFISCDELEN 130

Query: 121 VLLRLELWDSNRSDSE-CRNMIRAYDTNLDGKLDFEEFKNMMLLTT 166
           VL RLELWD+NR+D + CR+MIRAYDTNLDGKLDFEEFKNMMLLTT
Sbjct: 131 VLSRLELWDANRNDIDYCRSMIRAYDTNLDGKLDFEEFKNMMLLTT 170

BLAST of Moc04g31580 vs. NCBI nr
Match: XP_008441608.1 (PREDICTED: probable calcium-binding protein CML44 [Cucumis melo] >TYJ95710.1 putative calcium-binding protein CML44 [Cucumis melo var. makuwa])

HSP 1 Score: 250.8 bits (639), Expect = 8.5e-63
Identity = 128/166 (77.11%), Postives = 144/166 (86.75%), Query Frame = 0

Query: 1   MSPICSNDLLKIFNKLDKNCDGLVCLQELHWLLDRIGIQLTMEELESSLEKPSLDFNEFL 60
           MSPI S DL +IF KLDKNCDGL+CLQEL WLLDRIGIQLTMEELES LEKPSLDF+EFL
Sbjct: 1   MSPISSKDLHRIFKKLDKNCDGLLCLQELKWLLDRIGIQLTMEELESFLEKPSLDFDEFL 60

Query: 61  FFYESISKRNKGECNGGVGDDENEMLDGHEGEEDVDIVYMAFKVFDMNDDGFISCEELRS 120
           FFYESISK+NKGEC GGV      + D H  E+D +IVYMAFKVFDMN DGFISC+EL +
Sbjct: 61  FFYESISKQNKGECKGGVA---GCVQDDH--EDDTEIVYMAFKVFDMNGDGFISCDELEN 120

Query: 121 VLLRLELWDSNRSDSE-CRNMIRAYDTNLDGKLDFEEFKNMMLLTT 166
           VL+RLELWD++RS+ + CR+MI+AYDTNLDGKLDFEEFKNMMLLTT
Sbjct: 121 VLVRLELWDASRSEVDYCRSMIQAYDTNLDGKLDFEEFKNMMLLTT 161

BLAST of Moc04g31580 vs. NCBI nr
Match: XP_004149438.1 (probable calcium-binding protein CML44 [Cucumis sativus] >KGN47684.1 hypothetical protein Csa_018262 [Cucumis sativus])

HSP 1 Score: 250.4 bits (638), Expect = 1.1e-62
Identity = 124/166 (74.70%), Postives = 145/166 (87.35%), Query Frame = 0

Query: 1   MSPICSNDLLKIFNKLDKNCDGLVCLQELHWLLDRIGIQLTMEELESSLEKPSLDFNEFL 60
           M PI S DL +IF KLDKNCDGL+CLQEL+WLLD IGIQLTMEELES LE+PSLDF+EFL
Sbjct: 1   MPPISSKDLHRIFKKLDKNCDGLICLQELNWLLDSIGIQLTMEELESFLERPSLDFDEFL 60

Query: 61  FFYESISKRNKGECNGGVGDDENEMLDGHEGEEDVDIVYMAFKVFDMNDDGFISCEELRS 120
           FFYESISK+NKGEC GGV     +  D  +G++D++IVY+AFKVFDMN DGFISC+EL +
Sbjct: 61  FFYESISKQNKGECKGGVAGCVQDN-DNDQGQDDMEIVYLAFKVFDMNGDGFISCDELEN 120

Query: 121 VLLRLELWDSNRSDSE-CRNMIRAYDTNLDGKLDFEEFKNMMLLTT 166
           VL++LELWD++RSD + CR+MIRAYDTNLDGKLDFEEFKNMMLLTT
Sbjct: 121 VLVKLELWDASRSDVDYCRSMIRAYDTNLDGKLDFEEFKNMMLLTT 165

BLAST of Moc04g31580 vs. NCBI nr
Match: KAG6602129.1 (putative calcium-binding protein CML44, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 250.0 bits (637), Expect = 1.4e-62
Identity = 127/167 (76.05%), Postives = 142/167 (85.03%), Query Frame = 0

Query: 1   MSPICS-NDLLKIFNKLDKNCDGLVCLQELHWLLDRIGIQLTMEELESSLEKPSLDFNEF 60
           MSPI S NDLL+IFNKLDKN DGL+CL EL+WLLD IG++LTME+L S LEKPSLDFNEF
Sbjct: 1   MSPIISINDLLRIFNKLDKNRDGLICLHELNWLLDTIGVELTMEDLHSFLEKPSLDFNEF 60

Query: 61  LFFYESISKRNKGECNGGVGDDENEMLDGHEGEEDVDIVYMAFKVFDMNDDGFISCEELR 120
           LFFYESISK+NKGEC GGV        D +  EEDVDIVYMAFKVFDMNDDGFISCEEL+
Sbjct: 61  LFFYESISKQNKGECKGGVS-------DVNAREEDVDIVYMAFKVFDMNDDGFISCEELQ 120

Query: 121 SVLLRLELWDSNRSDSECRNMIRAYDTNLDGKLDFEEFKNMMLLTTS 167
            VL RLELWD N +D++C++MIRAYDTNLDG LDFEEFKNMM LT+S
Sbjct: 121 LVLSRLELWDVNTTDTDCKSMIRAYDTNLDGMLDFEEFKNMMSLTSS 160

BLAST of Moc04g31580 vs. ExPASy Swiss-Prot
Match: Q9LPK5 (Probable calcium-binding protein CML44 OS=Arabidopsis thaliana OX=3702 GN=CML44 PE=2 SV=2)

HSP 1 Score: 117.9 bits (294), Expect = 1.1e-25
Identity = 73/161 (45.34%), Postives = 98/161 (60.87%), Query Frame = 0

Query: 4   ICSNDLLKIFNKLDKNCDGLVCLQELHWLLDRIG-IQLTMEELESSLEKPSLDFNEFL-F 63
           I +NDL ++F  LDKN DGLV L EL W+LD++G  + T +ELE  + K SLD +EFL F
Sbjct: 6   ITTNDLRRMFKTLDKNQDGLVTLDELLWILDKLGWAEHTPDELELIVGKQSLDLDEFLRF 65

Query: 64  FYESISKRNKGECNGGVGDDENEMLDGHEGEEDVDIVYMAFKVFDMNDDGFISCEELRSV 123
           +Y+++      + N  V  D +E             +  AF VFD+N DG+IS EELR V
Sbjct: 66  YYDAVLDSKGSKKNIDVVADNDE------------AIARAFNVFDVNGDGYISAEELRDV 125

Query: 124 LLRLELWDSNRSDSECRNMIRAYDTNLDGKLDFEEFKNMML 163
           L RL  ++      +C  MIR +D NLDG +DFEEFKNM+L
Sbjct: 126 LERLG-FEEEAKAWDCGRMIRVHDKNLDGFVDFEEFKNMIL 153

BLAST of Moc04g31580 vs. ExPASy Swiss-Prot
Match: Q9SVG9 (Calcium-binding protein CML42 OS=Arabidopsis thaliana OX=3702 GN=CML42 PE=1 SV=1)

HSP 1 Score: 90.9 bits (224), Expect = 1.5e-17
Identity = 59/159 (37.11%), Postives = 91/159 (57.23%), Query Frame = 0

Query: 9   LLKIFNKLDKNCDGLVCLQELHWLLDRIGIQLTMEELESSLE------KPSLDFNEFLFF 68
           L +IF+  DKN DG + ++EL   L R+G+   + +L+S++E         L+F++F   
Sbjct: 30  LQRIFDLFDKNGDGFITVEELSQALTRLGLNADLSDLKSTVESYIQPGNTGLNFDDFSSL 89

Query: 69  YESISKRNKGECNGGVGDDENEMLDGHEGEEDVDIVYMAFKVFDMNDDGFISCEELRSVL 128
           ++++     G   GG G++E++     E E D   +  AFKVFD N DGFIS  EL++VL
Sbjct: 90  HKTLDDSFFGGACGG-GENEDDPSSAAENESD---LAEAFKVFDENGDGFISARELQTVL 149

Query: 129 LRLELWDSNRSDSECRNMIRAYDTNLDGKLDFEEFKNMM 162
            +L L +    +     MI + D N DG++DF EFKNMM
Sbjct: 150 KKLGLPEGGEME-RVEKMIVSVDRNQDGRVDFFEFKNMM 183

BLAST of Moc04g31580 vs. ExPASy Swiss-Prot
Match: Q2QVI1 (Probable calcium-binding protein CML28 OS=Oryza sativa subsp. japonica OX=39947 GN=CML28 PE=2 SV=1)

HSP 1 Score: 85.9 bits (211), Expect = 4.7e-16
Identity = 63/173 (36.42%), Postives = 86/173 (49.71%), Query Frame = 0

Query: 6   SNDLLKIFNKLDKNCDGLVCLQELHWLLDRIGIQLTMEELESSLEK------PSLDFNEF 65
           S +L K+F   DKN DG +  +EL       GI +  +EL+++++K        +D  EF
Sbjct: 3   STELRKVFKMFDKNGDGRITKKELGESFKNFGIFIPDDELDATMDKIDANGDGCVDVEEF 62

Query: 66  LFFYESI--------SKRNKGECNGGVG---DDENEMLDGHEGEEDVDIVYMAFKVFDMN 125
              Y SI        + R      GG G   DDE+E +              AF VFD N
Sbjct: 63  GLLYRSILGDDAAGRAPRTAAAAIGGEGGAPDDEDEGM------------REAFNVFDQN 122

Query: 126 DDGFISCEELRSVLLRLELWDSNRSDSECRNMIRAYDTNLDGKLDFEEFKNMM 162
            DGFI+ +ELRSVL  L L    R+  +CR MI   D + DG++DF+EFK MM
Sbjct: 123 GDGFITVDELRSVLSSLGL-KHGRTADDCRRMISMVDADGDGRVDFKEFKQMM 162

BLAST of Moc04g31580 vs. ExPASy Swiss-Prot
Match: P43187 (Calcium-binding allergen Bet v 3 OS=Betula pendula OX=3505 GN=BETVIII PE=1 SV=1)

HSP 1 Score: 82.8 bits (203), Expect = 4.0e-15
Identity = 56/161 (34.78%), Postives = 92/161 (57.14%), Query Frame = 0

Query: 9   LLKIFNKLDKNCDGLVCLQELHWLLDRIGIQLTMEELESSLE------KPSLDFNEFLFF 68
           L +IF+  DKN DG++ + EL   L+ +G++  + ELES+++         L F +F+  
Sbjct: 41  LRRIFDLFDKNSDGIITVDELSRALNLLGLETDLSELESTVKSFTREGNIGLQFEDFISL 100

Query: 69  YESISKRNKGECNGGVGDDENE--MLDGHEGEEDVDIVYMAFKVFDMNDDGFISCEELRS 128
           ++S++  +     GG  +D+NE  M      +E+ D  +  FKVFD + DG+IS  EL+ 
Sbjct: 101 HQSLN--DSYFAYGGEDEDDNEEDMRKSILSQEEAD-SFGGFKVFDEDGDGYISARELQM 160

Query: 129 VLLRLELWDSNRSDSECRNMIRAYDTNLDGKLDFEEFKNMM 162
           VL +L   + +  D     MI + D+N DG++DF EFK+MM
Sbjct: 161 VLGKLGFSEGSEID-RVEKMIVSVDSNRDGRVDFFEFKDMM 197

BLAST of Moc04g31580 vs. ExPASy Swiss-Prot
Match: Q9SRR7 (Calmodulin-like protein 3 OS=Arabidopsis thaliana OX=3702 GN=CML3 PE=2 SV=1)

HSP 1 Score: 82.0 bits (201), Expect = 6.8e-15
Identity = 57/160 (35.62%), Postives = 82/160 (51.25%), Query Frame = 0

Query: 8   DLLKIFNKLDKNCDGLVCLQELHWLLDRIGIQLTMEELESSLEKPSL------DFNEFLF 67
           +L +IF   D+N DG +  QEL+  L+ +GI +  ++L   +EK  L      D  EF  
Sbjct: 5   ELARIFQMFDRNGDGKITKQELNDSLENLGIYIPDKDLVQMIEKIDLNGDGYVDIEEFGG 64

Query: 68  FYESISKRNKGECNGGVGDDENEMLDGHEGEEDVDIVYMAFKVFDMNDDGFISCEELRSV 127
            Y++I +           D+E +M +             AF VFD N DGFI+ EELRSV
Sbjct: 65  LYQTIMEER---------DEEEDMRE-------------AFNVFDQNRDGFITVEELRSV 124

Query: 128 LLRLELWDSNRSDSECRNMIRAYDTNLDGKLDFEEFKNMM 162
           L  L L    R+  +C+ MI   D + DG ++F+EFK MM
Sbjct: 125 LASLGL-KQGRTLEDCKRMISKVDVDGDGMVNFKEFKQMM 141

BLAST of Moc04g31580 vs. ExPASy TrEMBL
Match: A0A6J1CJ36 (probable calcium-binding protein CML44 OS=Momordica charantia OX=3673 GN=LOC111011820 PE=4 SV=1)

HSP 1 Score: 340.1 bits (871), Expect = 5.1e-90
Identity = 166/166 (100.00%), Postives = 166/166 (100.00%), Query Frame = 0

Query: 1   MSPICSNDLLKIFNKLDKNCDGLVCLQELHWLLDRIGIQLTMEELESSLEKPSLDFNEFL 60
           MSPICSNDLLKIFNKLDKNCDGLVCLQELHWLLDRIGIQLTMEELESSLEKPSLDFNEFL
Sbjct: 1   MSPICSNDLLKIFNKLDKNCDGLVCLQELHWLLDRIGIQLTMEELESSLEKPSLDFNEFL 60

Query: 61  FFYESISKRNKGECNGGVGDDENEMLDGHEGEEDVDIVYMAFKVFDMNDDGFISCEELRS 120
           FFYESISKRNKGECNGGVGDDENEMLDGHEGEEDVDIVYMAFKVFDMNDDGFISCEELRS
Sbjct: 61  FFYESISKRNKGECNGGVGDDENEMLDGHEGEEDVDIVYMAFKVFDMNDDGFISCEELRS 120

Query: 121 VLLRLELWDSNRSDSECRNMIRAYDTNLDGKLDFEEFKNMMLLTTS 167
           VLLRLELWDSNRSDSECRNMIRAYDTNLDGKLDFEEFKNMMLLTTS
Sbjct: 121 VLLRLELWDSNRSDSECRNMIRAYDTNLDGKLDFEEFKNMMLLTTS 166

BLAST of Moc04g31580 vs. ExPASy TrEMBL
Match: A0A5D3B6U1 (Putative calcium-binding protein CML44 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold282G00080 PE=4 SV=1)

HSP 1 Score: 250.8 bits (639), Expect = 4.1e-63
Identity = 128/166 (77.11%), Postives = 144/166 (86.75%), Query Frame = 0

Query: 1   MSPICSNDLLKIFNKLDKNCDGLVCLQELHWLLDRIGIQLTMEELESSLEKPSLDFNEFL 60
           MSPI S DL +IF KLDKNCDGL+CLQEL WLLDRIGIQLTMEELES LEKPSLDF+EFL
Sbjct: 1   MSPISSKDLHRIFKKLDKNCDGLLCLQELKWLLDRIGIQLTMEELESFLEKPSLDFDEFL 60

Query: 61  FFYESISKRNKGECNGGVGDDENEMLDGHEGEEDVDIVYMAFKVFDMNDDGFISCEELRS 120
           FFYESISK+NKGEC GGV      + D H  E+D +IVYMAFKVFDMN DGFISC+EL +
Sbjct: 61  FFYESISKQNKGECKGGVA---GCVQDDH--EDDTEIVYMAFKVFDMNGDGFISCDELEN 120

Query: 121 VLLRLELWDSNRSDSE-CRNMIRAYDTNLDGKLDFEEFKNMMLLTT 166
           VL+RLELWD++RS+ + CR+MI+AYDTNLDGKLDFEEFKNMMLLTT
Sbjct: 121 VLVRLELWDASRSEVDYCRSMIQAYDTNLDGKLDFEEFKNMMLLTT 161

BLAST of Moc04g31580 vs. ExPASy TrEMBL
Match: A0A1S3B3T2 (probable calcium-binding protein CML44 OS=Cucumis melo OX=3656 GN=LOC103485689 PE=4 SV=1)

HSP 1 Score: 250.8 bits (639), Expect = 4.1e-63
Identity = 128/166 (77.11%), Postives = 144/166 (86.75%), Query Frame = 0

Query: 1   MSPICSNDLLKIFNKLDKNCDGLVCLQELHWLLDRIGIQLTMEELESSLEKPSLDFNEFL 60
           MSPI S DL +IF KLDKNCDGL+CLQEL WLLDRIGIQLTMEELES LEKPSLDF+EFL
Sbjct: 1   MSPISSKDLHRIFKKLDKNCDGLLCLQELKWLLDRIGIQLTMEELESFLEKPSLDFDEFL 60

Query: 61  FFYESISKRNKGECNGGVGDDENEMLDGHEGEEDVDIVYMAFKVFDMNDDGFISCEELRS 120
           FFYESISK+NKGEC GGV      + D H  E+D +IVYMAFKVFDMN DGFISC+EL +
Sbjct: 61  FFYESISKQNKGECKGGVA---GCVQDDH--EDDTEIVYMAFKVFDMNGDGFISCDELEN 120

Query: 121 VLLRLELWDSNRSDSE-CRNMIRAYDTNLDGKLDFEEFKNMMLLTT 166
           VL+RLELWD++RS+ + CR+MI+AYDTNLDGKLDFEEFKNMMLLTT
Sbjct: 121 VLVRLELWDASRSEVDYCRSMIQAYDTNLDGKLDFEEFKNMMLLTT 161

BLAST of Moc04g31580 vs. ExPASy TrEMBL
Match: A0A0A0KFQ6 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G376250 PE=4 SV=1)

HSP 1 Score: 250.4 bits (638), Expect = 5.4e-63
Identity = 124/166 (74.70%), Postives = 145/166 (87.35%), Query Frame = 0

Query: 1   MSPICSNDLLKIFNKLDKNCDGLVCLQELHWLLDRIGIQLTMEELESSLEKPSLDFNEFL 60
           M PI S DL +IF KLDKNCDGL+CLQEL+WLLD IGIQLTMEELES LE+PSLDF+EFL
Sbjct: 1   MPPISSKDLHRIFKKLDKNCDGLICLQELNWLLDSIGIQLTMEELESFLERPSLDFDEFL 60

Query: 61  FFYESISKRNKGECNGGVGDDENEMLDGHEGEEDVDIVYMAFKVFDMNDDGFISCEELRS 120
           FFYESISK+NKGEC GGV     +  D  +G++D++IVY+AFKVFDMN DGFISC+EL +
Sbjct: 61  FFYESISKQNKGECKGGVAGCVQDN-DNDQGQDDMEIVYLAFKVFDMNGDGFISCDELEN 120

Query: 121 VLLRLELWDSNRSDSE-CRNMIRAYDTNLDGKLDFEEFKNMMLLTT 166
           VL++LELWD++RSD + CR+MIRAYDTNLDGKLDFEEFKNMMLLTT
Sbjct: 121 VLVKLELWDASRSDVDYCRSMIRAYDTNLDGKLDFEEFKNMMLLTT 165

BLAST of Moc04g31580 vs. ExPASy TrEMBL
Match: A0A6J1FLU5 (probable calcium-binding protein CML44 OS=Cucurbita moschata OX=3662 GN=LOC111445399 PE=4 SV=1)

HSP 1 Score: 248.8 bits (634), Expect = 1.6e-62
Identity = 123/162 (75.93%), Postives = 140/162 (86.42%), Query Frame = 0

Query: 3   PICSNDLLKIFNKLDKNCDGLVCLQELHWLLDRIGIQLTMEELESSLEKPSLDFNEFLFF 62
           PI SNDLL+IFN+LDKN DGL+C+QEL WLLD IGIQLTMEELES LEKPSLDF+EFLFF
Sbjct: 4   PISSNDLLRIFNRLDKNKDGLICVQELKWLLDTIGIQLTMEELESFLEKPSLDFDEFLFF 63

Query: 63  YESISKRNKGECNGGVGDDENEMLDGHEGEEDVDIVYMAFKVFDMNDDGFISCEELRSVL 122
           YESISK+NKGEC GG+ D+        + EEDV+IVYMAFKVFD+N DGFISCEEL +VL
Sbjct: 64  YESISKQNKGECKGGLVDEHG------DEEEDVEIVYMAFKVFDVNGDGFISCEELENVL 123

Query: 123 LRLELWDSNRSDSECRNMIRAYDTNLDGKLDFEEFKNMMLLT 165
            RLELWD+N+S+ +CR+MIRAYDTNLDGKLDFEEF NMML T
Sbjct: 124 SRLELWDANKSEVDCRSMIRAYDTNLDGKLDFEEFMNMMLFT 159

BLAST of Moc04g31580 vs. TAIR 10
Match: AT1G21550.1 (Calcium-binding EF-hand family protein )

HSP 1 Score: 117.9 bits (294), Expect = 8.0e-27
Identity = 73/161 (45.34%), Postives = 98/161 (60.87%), Query Frame = 0

Query: 4   ICSNDLLKIFNKLDKNCDGLVCLQELHWLLDRIG-IQLTMEELESSLEKPSLDFNEFL-F 63
           I +NDL ++F  LDKN DGLV L EL W+LD++G  + T +ELE  + K SLD +EFL F
Sbjct: 6   ITTNDLRRMFKTLDKNQDGLVTLDELLWILDKLGWAEHTPDELELIVGKQSLDLDEFLRF 65

Query: 64  FYESISKRNKGECNGGVGDDENEMLDGHEGEEDVDIVYMAFKVFDMNDDGFISCEELRSV 123
           +Y+++      + N  V  D +E             +  AF VFD+N DG+IS EELR V
Sbjct: 66  YYDAVLDSKGSKKNIDVVADNDE------------AIARAFNVFDVNGDGYISAEELRDV 125

Query: 124 LLRLELWDSNRSDSECRNMIRAYDTNLDGKLDFEEFKNMML 163
           L RL  ++      +C  MIR +D NLDG +DFEEFKNM+L
Sbjct: 126 LERLG-FEEEAKAWDCGRMIRVHDKNLDGFVDFEEFKNMIL 153

BLAST of Moc04g31580 vs. TAIR 10
Match: AT4G20780.1 (calmodulin like 42 )

HSP 1 Score: 90.9 bits (224), Expect = 1.0e-18
Identity = 59/159 (37.11%), Postives = 91/159 (57.23%), Query Frame = 0

Query: 9   LLKIFNKLDKNCDGLVCLQELHWLLDRIGIQLTMEELESSLE------KPSLDFNEFLFF 68
           L +IF+  DKN DG + ++EL   L R+G+   + +L+S++E         L+F++F   
Sbjct: 30  LQRIFDLFDKNGDGFITVEELSQALTRLGLNADLSDLKSTVESYIQPGNTGLNFDDFSSL 89

Query: 69  YESISKRNKGECNGGVGDDENEMLDGHEGEEDVDIVYMAFKVFDMNDDGFISCEELRSVL 128
           ++++     G   GG G++E++     E E D   +  AFKVFD N DGFIS  EL++VL
Sbjct: 90  HKTLDDSFFGGACGG-GENEDDPSSAAENESD---LAEAFKVFDENGDGFISARELQTVL 149

Query: 129 LRLELWDSNRSDSECRNMIRAYDTNLDGKLDFEEFKNMM 162
            +L L +    +     MI + D N DG++DF EFKNMM
Sbjct: 150 KKLGLPEGGEME-RVEKMIVSVDRNQDGRVDFFEFKNMM 183

BLAST of Moc04g31580 vs. TAIR 10
Match: AT3G07490.1 (ARF-GAP domain 11 )

HSP 1 Score: 82.0 bits (201), Expect = 4.9e-16
Identity = 57/160 (35.62%), Postives = 82/160 (51.25%), Query Frame = 0

Query: 8   DLLKIFNKLDKNCDGLVCLQELHWLLDRIGIQLTMEELESSLEKPSL------DFNEFLF 67
           +L +IF   D+N DG +  QEL+  L+ +GI +  ++L   +EK  L      D  EF  
Sbjct: 5   ELARIFQMFDRNGDGKITKQELNDSLENLGIYIPDKDLVQMIEKIDLNGDGYVDIEEFGG 64

Query: 68  FYESISKRNKGECNGGVGDDENEMLDGHEGEEDVDIVYMAFKVFDMNDDGFISCEELRSV 127
            Y++I +           D+E +M +             AF VFD N DGFI+ EELRSV
Sbjct: 65  LYQTIMEER---------DEEEDMRE-------------AFNVFDQNRDGFITVEELRSV 124

Query: 128 LLRLELWDSNRSDSECRNMIRAYDTNLDGKLDFEEFKNMM 162
           L  L L    R+  +C+ MI   D + DG ++F+EFK MM
Sbjct: 125 LASLGL-KQGRTLEDCKRMISKVDVDGDGMVNFKEFKQMM 141

BLAST of Moc04g31580 vs. TAIR 10
Match: AT4G12860.1 (EF hand calcium-binding protein family )

HSP 1 Score: 77.4 bits (189), Expect = 1.2e-14
Identity = 54/160 (33.75%), Postives = 79/160 (49.38%), Query Frame = 0

Query: 8   DLLKIFNKLDKNCDGLVCLQELHWLLDRIGIQLTMEELESSLEK------PSLDFNEFLF 67
           +L ++F   DKN DG +   EL      +GI +   E+   + K       ++D +EF  
Sbjct: 5   ELSRVFQMFDKNGDGKIAKNELKDFFKSVGIMVPENEINEMIAKMDVNGDGAMDIDEFGS 64

Query: 68  FYESISKRNKGECNGGVGDDENEMLDGHEGEEDVDIVYMAFKVFDMNDDGFISCEELRSV 127
            Y+                   EM++  E EED   +  AF+VFD N DGFI+ EELRSV
Sbjct: 65  LYQ-------------------EMVEEKEEEED---MREAFRVFDQNGDGFITDEELRSV 124

Query: 128 LLRLELWDSNRSDSECRNMIRAYDTNLDGKLDFEEFKNMM 162
           L  + L    R+  +C+ MI   D + DG ++F+EFK MM
Sbjct: 125 LASMGL-KQGRTLEDCKKMISKVDVDGDGMVNFKEFKQMM 141

BLAST of Moc04g31580 vs. TAIR 10
Match: AT5G44460.1 (calmodulin like 43 )

HSP 1 Score: 77.4 bits (189), Expect = 1.2e-14
Identity = 54/159 (33.96%), Postives = 87/159 (54.72%), Query Frame = 0

Query: 9   LLKIFNKLDKNCDGLVCLQELHWLLDRIGIQLTMEELESSL------EKPSLDFNEFLFF 68
           L ++F+  DKN DG + ++EL   L R+G+     +L+S++      +K  L F++F   
Sbjct: 29  LHRVFDLFDKNNDGFITVEELSQALSRLGLDADFSDLKSTVDSFIKPDKTGLRFDDFAAL 88

Query: 69  YESISKRNKGECNGGVGDDENEMLDGHEGEEDVDIVYMAFKVFDMNDDGFISCEELRSVL 128
           ++++ +   G         E    DG   E D++    AF VFD + DGFIS  EL+ VL
Sbjct: 89  HKTLDESFFG--------GEGSCCDG-SPESDLE---EAFNVFDEDGDGFISAVELQKVL 148

Query: 129 LRLELWDSNRSDSECRNMIRAYDTNLDGKLDFEEFKNMM 162
            +L L ++   + +   MI + D+N DG++DF EFKNMM
Sbjct: 149 KKLGLPEAGEIE-QVEKMIVSVDSNHDGRVDFFEFKNMM 174

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022141411.11.1e-89100.00probable calcium-binding protein CML44 [Momordica charantia][more]
XP_038885742.13.8e-6378.31probable calcium-binding protein CML44 [Benincasa hispida][more]
XP_008441608.18.5e-6377.11PREDICTED: probable calcium-binding protein CML44 [Cucumis melo] >TYJ95710.1 put... [more]
XP_004149438.11.1e-6274.70probable calcium-binding protein CML44 [Cucumis sativus] >KGN47684.1 hypothetica... [more]
KAG6602129.11.4e-6276.05putative calcium-binding protein CML44, partial [Cucurbita argyrosperma subsp. s... [more]
Match NameE-valueIdentityDescription
Q9LPK51.1e-2545.34Probable calcium-binding protein CML44 OS=Arabidopsis thaliana OX=3702 GN=CML44 ... [more]
Q9SVG91.5e-1737.11Calcium-binding protein CML42 OS=Arabidopsis thaliana OX=3702 GN=CML42 PE=1 SV=1[more]
Q2QVI14.7e-1636.42Probable calcium-binding protein CML28 OS=Oryza sativa subsp. japonica OX=39947 ... [more]
P431874.0e-1534.78Calcium-binding allergen Bet v 3 OS=Betula pendula OX=3505 GN=BETVIII PE=1 SV=1[more]
Q9SRR76.8e-1535.63Calmodulin-like protein 3 OS=Arabidopsis thaliana OX=3702 GN=CML3 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1CJ365.1e-90100.00probable calcium-binding protein CML44 OS=Momordica charantia OX=3673 GN=LOC1110... [more]
A0A5D3B6U14.1e-6377.11Putative calcium-binding protein CML44 OS=Cucumis melo var. makuwa OX=1194695 GN... [more]
A0A1S3B3T24.1e-6377.11probable calcium-binding protein CML44 OS=Cucumis melo OX=3656 GN=LOC103485689 P... [more]
A0A0A0KFQ65.4e-6374.70Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G376250 PE=4 SV=1[more]
A0A6J1FLU51.6e-6275.93probable calcium-binding protein CML44 OS=Cucurbita moschata OX=3662 GN=LOC11144... [more]
Match NameE-valueIdentityDescription
AT1G21550.18.0e-2745.34Calcium-binding EF-hand family protein [more]
AT4G20780.11.0e-1837.11calmodulin like 42 [more]
AT3G07490.14.9e-1635.63ARF-GAP domain 11 [more]
AT4G12860.11.2e-1433.75EF hand calcium-binding protein family [more]
AT5G44460.11.2e-1433.96calmodulin like 43 [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (OHB3-1) v2
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002048EF-hand domainSMARTSM00054efh_1coord: 136..164
e-value: 3.4E-4
score: 29.9
coord: 97..125
e-value: 0.81
score: 18.5
coord: 8..36
e-value: 0.023
score: 23.8
IPR002048EF-hand domainPFAMPF13499EF-hand_7coord: 99..161
e-value: 1.3E-10
score: 41.5
IPR002048EF-hand domainPFAMPF13202EF-hand_5coord: 11..29
e-value: 0.037
score: 13.5
IPR002048EF-hand domainPROSITEPS50222EF_HAND_2coord: 132..166
score: 12.170192
IPR002048EF-hand domainPROSITEPS50222EF_HAND_2coord: 93..128
score: 11.919133
IPR002048EF-hand domainPROSITEPS50222EF_HAND_2coord: 4..39
score: 10.580154
IPR002048EF-hand domainCDDcd00051EFhcoord: 101..161
e-value: 1.09648E-12
score: 57.9429
NoneNo IPR availableGENE3D1.10.238.10coord: 6..68
e-value: 8.0E-7
score: 31.1
NoneNo IPR availableGENE3D1.10.238.10coord: 98..162
e-value: 2.5E-18
score: 68.1
NoneNo IPR availablePANTHERPTHR10891:SF788CALCIUM-BINDING PROTEIN CML44-RELATEDcoord: 1..166
NoneNo IPR availablePANTHERPTHR10891EF-HAND CALCIUM-BINDING DOMAIN CONTAINING PROTEINcoord: 1..166
IPR018247EF-Hand 1, calcium-binding sitePROSITEPS00018EF_HAND_1coord: 17..29
IPR018247EF-Hand 1, calcium-binding sitePROSITEPS00018EF_HAND_1coord: 106..118
IPR011992EF-hand domain pairSUPERFAMILY47473EF-handcoord: 3..163

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Moc04g31580.1Moc04g31580.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0005509 calcium ion binding