Moc04g31320 (gene) Bitter gourd (OHB3-1) v2

Overview
NameMoc04g31320
Typegene
OrganismMomordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
DescriptionDelta-aminolevulinic acid dehydratase
Locationchr4: 23497079 .. 23502275 (-)
RNA-Seq ExpressionMoc04g31320
SyntenyMoc04g31320
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCTTCAACAGTTCTTAATGCGCCTAATTGTGTTCGAACAATTAACGGTCTGGATTGCGGCTCCTATGTTGGGCTAAAACCTTCGCCAAATTCAAATTTCCTCTGCGTCCGGACCCCGGCCAATGTGCGTTCTCCGCGTCTTTTCGTTGTGAGAGCTAGCGACGAGCGCGATGCACATATGAAGAAGCTCGGACGGAGTGATGCAGAGTGTGAAGCTGCTGTTGTGGCAGGCAACGTTCCAGAAGCACCTCCTGTGCCGCCGAAACCAGCAGCGCCTGCTGGAACTCCTGTAGCTCCTTTGCTGGTTAGTGTTGATGATATAACTGGATTTTCACATAAACTTTTTCATACTATGAATCGACATATGAAATTATAAGTAGACGTCCTTAGTTTTAGAGATAATGTTATCTGTGCACCTGAATCGTGATTATGCATTCGAGAGTTCAAATAACAAATTCCACTGAAAGAACTATTAAGCATTATGCTGTATTACTAATTTTCTACATTCACGAACTGTTTAACATTAAAAATGAGTGCTCCCTTCTCCTCACTTACAACAAATTTTCTATCAGCCTCTCAGCCGTCGTCCTCGACGTAACCGGAGGTCACCCGCACTCAGAGCATCATTTCAGGAAACCAGCCTATCACCTTCAAATTTTGTATATCCTTTGTTCATTCATGAAGGTACGCACTATGCAATCATAATCCGTTTTTTCTTTTATTATTTGTATTACTCTGTTTTGGTGTGGCGCTGTGTGCATGGCCTTGTGTAGTTTGTAGGCAAGGTAGGCCTAAACATTAGGAAATTTGCAGGTGAGGAGGATACACCGATTGGAGCTATGCCTGGATGCTATAGGCTTGGGTGGAGACATGGGCTTTTGGAAGAGGTAACTTAGCTACATGGGCTCTACTTTTCAGCAATCACTAATCACAAATGAGGTTGTTGCCTTGCTCAAAATTTCACCTTTGTATGTTCCTTGGTGGTCTGCTGCGTTTTTTTAGTCCCCGGTTGTTTGGATAGTTTTGGTGATTAAATCCAGCAACAGAATATGCTTTTAATATGGCACTTGCGTTTTGTTGCATGCAAAGGCAGTCAAAGTATGTTCCTCGAGCCTTTGAATTTTTGAATTTAAAAAATGCTCTTGTGGAATTGTAGTTTTTGGTTCCATGGAAAATTCCTTGCAGTATTCGATTCAAATATGGAATAAAGGAAAGGACGGCATTTTTTCATGTTGCTTTGTCAGCCCTTGGACCACAGAAAGCTTTTCTTTCTTGAATTTTGAGATGTACATGTTTAATAAATTGACCTTGATTTTATATTGCAGCATCGATTTAAAAGGAATTTACTTAGTCCGAGGCTCTGAGCAATACTTGAAAGAAAATTGAAAAACTGTCTTTTGGTGCTCAAGGGCAAAAATATTTGGACGAATCTTTCTTGTAGAAAGTTGTTTATGCAAAGTCATTCCTTTAAACGACAGAAAGAGATAGTTCAAGTTAAAGATGAACATGCATGTGTATCAGTTAAGAAGATAATCTTGTTTGAGGCAGGTTTCAAAGGCTCGGGATGTCGGTGTCAATTCTGTTGTGCTATTCCCTAAAGTTCCAGATGCATTGAAGGTTTTTACTCCTCATTCTTATACCATTCCTTTTTTTTTTTTTTGGTTTCTTAGTTGCATAATATAATCAGTTTTGCTTCAGTCTCCAACAGGGGACGAGGCATACAATGATAATGGTTTAGTGCCTCGAACAATTCGGTTGCTCAAGGACAAATACCCTGATCTTGTAAGTGAACTGTATTCTATTTGGATATTGATTTAAGTTATGGGAGTATTGTGGTCTCATGTGCAGAAACATTGCAATTATATATTGTACAACCCATATTTGTTCAGGTCATCTACACTGATGTTGCTTTGGATCCATACTCTTCTGATGGCCACGATGGTATAGTTAGAGAGGATGGTAAGAGTGATATACCTTTATTTAAATGATGCTAGAATAATCGTTGAAGCATTGTGTAGAAATTTTACGGGAATATTTCATTAATAATTGAGAAATTTTAGTTGAAACCAAAAACTTTTATAATATTAGAACGTTTGCCTTCGCATACACAATCTGAATATCTATCAAATGCATGGGTCTTTAATGGGTTAAGTCTAGATTCTGTTTGCTGATTAAATATTTGCAAGTAGAAACTATGTACCAGTACTGCATCCATCCCTATGCAAAGGTTTGCTCCAAATCTCATCTTGACGCAGAAGAAATTTTGAATGTAAAAAGAATCACATGTGCGAGCAAACGTGCCCATTTGCTTGACAAAGAGTAGTTTATGACTGTGAACTGCTTCGTAACGGTATCTATATGGACTGTTTTTGACTGAAATTCCCTCAAAATAAGCTCTATTTTCTAATGTGTAACGTAAGAACAACTATTGTAATTTTCAAGGGCAAAATATCTGCTTGGCAGCAGAGGCCGTTACTGAGACTCATCCTTTCAAAGTCAATTTGTTAAAATTTTATGGGCATTGACCTTACAATCGAATGTCTTCCTAGAATAATTTATTTTAGTTCAATATAGGTATATATGGTCACAAAGTTGGTTTCATATTTTGTAGGAGTTATAATGAATGATGAGACTGTTCATCAATTATGCAAACAAGCTGTTTCCCAGGTAATTTTATGAGATCGTTCTCTTTGTAAACTCAACCCTCGAACATGACAAATTCATGGCCCATTTATGAATTACAAAGTACTAAGGTTACGCAAATTTTCACAGCTATCTTTCAACATTTATTGCCTTTGTTTAATGGGATATTATATATGAGCACTTGTTCACTATTTTACCTTATATCTCCCCGTTTTGTATATGTCTCCTGGTGTCTTTTGAACAGATATTTATAGTTTGCTTTCTGTCTTAATTTCTTTGATCACTAGGCCCGTGCTGGAGCAGATGTTGTCAGTCCAAGTGACATGATGGATGGTAGGGTCGGAGCAATTAGAAGGGCTCTTGATGCTGAGGGCTTTCATCACGTCTCCATCATGTCTTACACGGCAAAGTAATTTCCTATTTTCCATTTCACCATTTTCTCCCGTTTTTGTTAGCCACATGAATAAGTACATTTAAAGCTTTGCTCTACTTCTAAAATTTGATTAGAATATGAATGCTGCCAGCTGCCGCCTGTCAAAATAAAAGCAAAATGATGTTTAATCCTGATGCTTTACAAAGTTATTAGTTCATTAGATCTTGTAGCTAGCCTAGATAGAAGAATTATTAGATGTCTCAATCAACTTTTGCCTCTAATATGTTTAAGGAATTTGGAAATCTTTTGTTAATTTGTAATGAGCTATATATTCCCCATTCGAGTCGGTTACTTAAAGAATCCTGCAGCTAAGCTAATGTCTCAATAAGATACCTGATGATGTATTTCCTTTCCATGTGGATCAAATCTTTCTTGAATATTGCTGAATTTATTTTCCACTCCTTTATCACTTAGAAGGTGTGTAGCATGGGGCACCTCTTTCACTCTTTCTACTCTACATCAACCTGGTTTATGTATGTCTAGCCAATTGGAATGAATAAAACTTTTAATTCCCTCCTTTTTTTTTTACATGAATGGTCCAGTTACTTTAATTTTTTACACAAACGATGTTTTACTTTTTTGTGCTTACCTTCATTTGGGGATTTTAAACAGGTATGCAAGTTCGTTTTATGGTCCATTTCGTGAAGCACTGGATTCAAATCCTCGTTTTGGCGACAAAAAGACGTATGTAGTATATTATATTATTATGAACTGAGATTTTACTTGCTCTAGGTTCTCACATACCAAACTAATCTGGCTTGCTTAACAAAAGTTACCAGATGAATCCAGCAAATTATAGAGAGGCTTTGATCGAAACCCGTGAAGATGAAGATGAAGGAGCTGATATTCTTCTGGTCAGTTGTCTTTTTTGGATCCTTTGAAATAATATACTTGGTGGTGAACTCCAATGTCGCTAACGTAAAGCTATTACTAATCGCTTAACAGGTGAAACCTGGTCTCCCTTATCTGGATATCATAAGGCTTCTCAGGAATAATTCTCCTTTACCTATTGCAGCATATCAGGTATTATTGACTATTTTGTTTTATGTTAATTATTATCTTTGCTTTACCTATTTTCATGTCCTATACACACCCCCATGTTCTACAATATGAGGCATTAATATCACATAAGATTGCATGACTCGAGTCTTATTTTGGAGAATGATAATTTTAATCGGGTTTGGACACTTGAAATATCACATAATTCTCCTTGGCCACGCATGATGATTAGCTTAGAATGCTGCTAGGAAATATAAAAGGATATATTTAGCACACATCAATAAGCGTCTTCCGCCTGATGTGGTTGACTAAATGCCGCTATGATCTAAATGAAGTAATAATTTATATTTTATAACCACTCTGTTTTGGTTTTCTATTTTTGAAAATGAAGTTTAAAATTACAGCTTTCACTTCTTCGTTTTATTATCTACTTTTTAAAAACGTTTTCAAGACTTAAGCCAAAATAAGAAAAGTAGTTTAAAAAATTTGTTTTTGTTTTTGAAATTTGATTAAGAATTTAAATTTGTTTTTAAGAGGTGAAAACATCCTAAAGAAATTGGGACGCAGCAGCGTAATTTTTAAAATCAGAGAAGTAAAAACAAAATTGTTATTTAAAGAGGTGGAAAGCAGCGGTCATGTAAATGATCCCACTTCTACGATTTCTAGACACTTGTATCATAATTGCATGCTTGATATCTGATAACTAACAAGGATGGTATTAAAGAAAATGGACGCAAATAAAACTTGAATGAATGCAATATGCGCATCTATGTATTTATAGGCCTTGAGGGATTGTTTGAAAATGAGGTTTAAAAAACAAACTTTTGTCTGTGTTTAGTAGAATTATTAGACACAAATTTTGTTATATTCAAATGCACTGCTCTTGAGTTAGTTCATATGACAAGTGATTAGAATATGCAATCATGTCGAATGATTTTACTATTTCGGTCAGGTTTCGGGCGAGTACTCGATGATCAAGGCTGGAGGGGTTCTCAAAATGATCGATGAAGAGAAGGTGATGATGGAGTCTCTGATGTGTCTCCGACGGGCAGGAGCAGACATAATCCTCACATATTTTGCTCTACAGGCAGCTAGATGTTTGTGTGGTGAGAAGAGGTGA

mRNA sequence

ATGGCTTCAACAGTTCTTAATGCGCCTAATTGTGTTCGAACAATTAACGGTCTGGATTGCGGCTCCTATGTTGGGCTAAAACCTTCGCCAAATTCAAATTTCCTCTGCGTCCGGACCCCGGCCAATGTGCGTTCTCCGCGTCTTTTCGTTGTGAGAGCTAGCGACGAGCGCGATGCACATATGAAGAAGCTCGGACGGAGTGATGCAGAGTGTGAAGCTGCTGTTGTGGCAGGCAACGTTCCAGAAGCACCTCCTGTGCCGCCGAAACCAGCAGCGCCTGCTGGAACTCCTGTAGCTCCTTTGCTGCCTCTCAGCCGTCGTCCTCGACGTAACCGGAGGTCACCCGCACTCAGAGCATCATTTCAGGAAACCAGCCTATCACCTTCAAATTTTGTATATCCTTTGTTCATTCATGAAGGTGAGGAGGATACACCGATTGGAGCTATGCCTGGATGCTATAGGCTTGGGTGGAGACATGGGCTTTTGGAAGAGGTTTCAAAGGCTCGGGATGTCGGTGTCAATTCTGTTGTGCTATTCCCTAAAGTTCCAGATGCATTGAAGTCTCCAACAGGGGACGAGGCATACAATGATAATGGTTTAGTGCCTCGAACAATTCGGTTGCTCAAGGACAAATACCCTGATCTTGTCATCTACACTGATGTTGCTTTGGATCCATACTCTTCTGATGGCCACGATGGTATAGTTAGAGAGGATGGAGTTATAATGAATGATGAGACTGTTCATCAATTATGCAAACAAGCTGTTTCCCAGGCCCGTGCTGGAGCAGATGTTGTCAGTCCAAGTGACATGATGGATGGTAGGGTCGGAGCAATTAGAAGGGCTCTTGATGCTGAGGGCTTTCATCACGTCTCCATCATGTCTTACACGGCAAAGTATGCAAGTTCGTTTTATGGTCCATTTCGTGAAGCACTGGATTCAAATCCTCGTTTTGGCGACAAAAAGACTTACCAGATGAATCCAGCAAATTATAGAGAGGCTTTGATCGAAACCCGTGAAGATGAAGATGAAGGAGCTGATATTCTTCTGGTGAAACCTGGTCTCCCTTATCTGGATATCATAAGGCTTCTCAGGAATAATTCTCCTTTACCTATTGCAGCATATCAGGTTTCGGGCGAGTACTCGATGATCAAGGCTGGAGGGGTTCTCAAAATGATCGATGAAGAGAAGGTGATGATGGAGTCTCTGATGTGTCTCCGACGGGCAGGAGCAGACATAATCCTCACATATTTTGCTCTACAGGCAGCTAGATGTTTGTGTGGTGAGAAGAGGTGA

Coding sequence (CDS)

ATGGCTTCAACAGTTCTTAATGCGCCTAATTGTGTTCGAACAATTAACGGTCTGGATTGCGGCTCCTATGTTGGGCTAAAACCTTCGCCAAATTCAAATTTCCTCTGCGTCCGGACCCCGGCCAATGTGCGTTCTCCGCGTCTTTTCGTTGTGAGAGCTAGCGACGAGCGCGATGCACATATGAAGAAGCTCGGACGGAGTGATGCAGAGTGTGAAGCTGCTGTTGTGGCAGGCAACGTTCCAGAAGCACCTCCTGTGCCGCCGAAACCAGCAGCGCCTGCTGGAACTCCTGTAGCTCCTTTGCTGCCTCTCAGCCGTCGTCCTCGACGTAACCGGAGGTCACCCGCACTCAGAGCATCATTTCAGGAAACCAGCCTATCACCTTCAAATTTTGTATATCCTTTGTTCATTCATGAAGGTGAGGAGGATACACCGATTGGAGCTATGCCTGGATGCTATAGGCTTGGGTGGAGACATGGGCTTTTGGAAGAGGTTTCAAAGGCTCGGGATGTCGGTGTCAATTCTGTTGTGCTATTCCCTAAAGTTCCAGATGCATTGAAGTCTCCAACAGGGGACGAGGCATACAATGATAATGGTTTAGTGCCTCGAACAATTCGGTTGCTCAAGGACAAATACCCTGATCTTGTCATCTACACTGATGTTGCTTTGGATCCATACTCTTCTGATGGCCACGATGGTATAGTTAGAGAGGATGGAGTTATAATGAATGATGAGACTGTTCATCAATTATGCAAACAAGCTGTTTCCCAGGCCCGTGCTGGAGCAGATGTTGTCAGTCCAAGTGACATGATGGATGGTAGGGTCGGAGCAATTAGAAGGGCTCTTGATGCTGAGGGCTTTCATCACGTCTCCATCATGTCTTACACGGCAAAGTATGCAAGTTCGTTTTATGGTCCATTTCGTGAAGCACTGGATTCAAATCCTCGTTTTGGCGACAAAAAGACTTACCAGATGAATCCAGCAAATTATAGAGAGGCTTTGATCGAAACCCGTGAAGATGAAGATGAAGGAGCTGATATTCTTCTGGTGAAACCTGGTCTCCCTTATCTGGATATCATAAGGCTTCTCAGGAATAATTCTCCTTTACCTATTGCAGCATATCAGGTTTCGGGCGAGTACTCGATGATCAAGGCTGGAGGGGTTCTCAAAATGATCGATGAAGAGAAGGTGATGATGGAGTCTCTGATGTGTCTCCGACGGGCAGGAGCAGACATAATCCTCACATATTTTGCTCTACAGGCAGCTAGATGTTTGTGTGGTGAGAAGAGGTGA

Protein sequence

MASTVLNAPNCVRTINGLDCGSYVGLKPSPNSNFLCVRTPANVRSPRLFVVRASDERDAHMKKLGRSDAECEAAVVAGNVPEAPPVPPKPAAPAGTPVAPLLPLSRRPRRNRRSPALRASFQETSLSPSNFVYPLFIHEGEEDTPIGAMPGCYRLGWRHGLLEEVSKARDVGVNSVVLFPKVPDALKSPTGDEAYNDNGLVPRTIRLLKDKYPDLVIYTDVALDPYSSDGHDGIVREDGVIMNDETVHQLCKQAVSQARAGADVVSPSDMMDGRVGAIRRALDAEGFHHVSIMSYTAKYASSFYGPFREALDSNPRFGDKKTYQMNPANYREALIETREDEDEGADILLVKPGLPYLDIIRLLRNNSPLPIAAYQVSGEYSMIKAGGVLKMIDEEKVMMESLMCLRRAGADIILTYFALQAARCLCGEKR
Homology
BLAST of Moc04g31320 vs. NCBI nr
Match: XP_022141587.1 (delta-aminolevulinic acid dehydratase, chloroplastic [Momordica charantia] >XP_022141588.1 delta-aminolevulinic acid dehydratase, chloroplastic [Momordica charantia])

HSP 1 Score: 864.0 bits (2231), Expect = 5.5e-247
Identity = 430/430 (100.00%), Postives = 430/430 (100.00%), Query Frame = 0

Query: 1   MASTVLNAPNCVRTINGLDCGSYVGLKPSPNSNFLCVRTPANVRSPRLFVVRASDERDAH 60
           MASTVLNAPNCVRTINGLDCGSYVGLKPSPNSNFLCVRTPANVRSPRLFVVRASDERDAH
Sbjct: 1   MASTVLNAPNCVRTINGLDCGSYVGLKPSPNSNFLCVRTPANVRSPRLFVVRASDERDAH 60

Query: 61  MKKLGRSDAECEAAVVAGNVPEAPPVPPKPAAPAGTPVAPLLPLSRRPRRNRRSPALRAS 120
           MKKLGRSDAECEAAVVAGNVPEAPPVPPKPAAPAGTPVAPLLPLSRRPRRNRRSPALRAS
Sbjct: 61  MKKLGRSDAECEAAVVAGNVPEAPPVPPKPAAPAGTPVAPLLPLSRRPRRNRRSPALRAS 120

Query: 121 FQETSLSPSNFVYPLFIHEGEEDTPIGAMPGCYRLGWRHGLLEEVSKARDVGVNSVVLFP 180
           FQETSLSPSNFVYPLFIHEGEEDTPIGAMPGCYRLGWRHGLLEEVSKARDVGVNSVVLFP
Sbjct: 121 FQETSLSPSNFVYPLFIHEGEEDTPIGAMPGCYRLGWRHGLLEEVSKARDVGVNSVVLFP 180

Query: 181 KVPDALKSPTGDEAYNDNGLVPRTIRLLKDKYPDLVIYTDVALDPYSSDGHDGIVREDGV 240
           KVPDALKSPTGDEAYNDNGLVPRTIRLLKDKYPDLVIYTDVALDPYSSDGHDGIVREDGV
Sbjct: 181 KVPDALKSPTGDEAYNDNGLVPRTIRLLKDKYPDLVIYTDVALDPYSSDGHDGIVREDGV 240

Query: 241 IMNDETVHQLCKQAVSQARAGADVVSPSDMMDGRVGAIRRALDAEGFHHVSIMSYTAKYA 300
           IMNDETVHQLCKQAVSQARAGADVVSPSDMMDGRVGAIRRALDAEGFHHVSIMSYTAKYA
Sbjct: 241 IMNDETVHQLCKQAVSQARAGADVVSPSDMMDGRVGAIRRALDAEGFHHVSIMSYTAKYA 300

Query: 301 SSFYGPFREALDSNPRFGDKKTYQMNPANYREALIETREDEDEGADILLVKPGLPYLDII 360
           SSFYGPFREALDSNPRFGDKKTYQMNPANYREALIETREDEDEGADILLVKPGLPYLDII
Sbjct: 301 SSFYGPFREALDSNPRFGDKKTYQMNPANYREALIETREDEDEGADILLVKPGLPYLDII 360

Query: 361 RLLRNNSPLPIAAYQVSGEYSMIKAGGVLKMIDEEKVMMESLMCLRRAGADIILTYFALQ 420
           RLLRNNSPLPIAAYQVSGEYSMIKAGGVLKMIDEEKVMMESLMCLRRAGADIILTYFALQ
Sbjct: 361 RLLRNNSPLPIAAYQVSGEYSMIKAGGVLKMIDEEKVMMESLMCLRRAGADIILTYFALQ 420

Query: 421 AARCLCGEKR 431
           AARCLCGEKR
Sbjct: 421 AARCLCGEKR 430

BLAST of Moc04g31320 vs. NCBI nr
Match: XP_038886111.1 (delta-aminolevulinic acid dehydratase, chloroplastic [Benincasa hispida])

HSP 1 Score: 834.7 bits (2155), Expect = 3.6e-238
Identity = 413/430 (96.05%), Postives = 424/430 (98.60%), Query Frame = 0

Query: 1   MASTVLNAPNCVRTINGLDCGSYVGLKPSPNSNFLCVRTPANVRSPRLFVVRASDERDAH 60
           MASTVLNAPN VR INGLDCGSYVGLK SPNSNFLCVRT ANVRSPRLFVVRA+DERDAH
Sbjct: 1   MASTVLNAPNSVRRINGLDCGSYVGLKRSPNSNFLCVRTSANVRSPRLFVVRATDERDAH 60

Query: 61  MKKLGRSDAECEAAVVAGNVPEAPPVPPKPAAPAGTPVAPLLPLSRRPRRNRRSPALRAS 120
           MKKLGRSDAECEAAVVAGNVPEAPPVPPKPA+PAGTPV PLLPLSRRPRRNRRSPA+RAS
Sbjct: 61  MKKLGRSDAECEAAVVAGNVPEAPPVPPKPASPAGTPVVPLLPLSRRPRRNRRSPAMRAS 120

Query: 121 FQETSLSPSNFVYPLFIHEGEEDTPIGAMPGCYRLGWRHGLLEEVSKARDVGVNSVVLFP 180
           FQET+LSPSNFVYPLFIHEGEEDTPIGAMPGCYRLGWRHGLLEEVSKARDVGVNS+VLFP
Sbjct: 121 FQETNLSPSNFVYPLFIHEGEEDTPIGAMPGCYRLGWRHGLLEEVSKARDVGVNSIVLFP 180

Query: 181 KVPDALKSPTGDEAYNDNGLVPRTIRLLKDKYPDLVIYTDVALDPYSSDGHDGIVREDGV 240
           KVPDALK+PTGDEAYNDNGLVPRTIRLLKD+YPDLVIYTDVALDPYSSDGHDGIVREDGV
Sbjct: 181 KVPDALKTPTGDEAYNDNGLVPRTIRLLKDRYPDLVIYTDVALDPYSSDGHDGIVREDGV 240

Query: 241 IMNDETVHQLCKQAVSQARAGADVVSPSDMMDGRVGAIRRALDAEGFHHVSIMSYTAKYA 300
           IMNDETVHQLCKQAVSQARAGADVVSPSDMMDGRVGAIRRALDAEGF+HVSIMSYTAKYA
Sbjct: 241 IMNDETVHQLCKQAVSQARAGADVVSPSDMMDGRVGAIRRALDAEGFYHVSIMSYTAKYA 300

Query: 301 SSFYGPFREALDSNPRFGDKKTYQMNPANYREALIETREDEDEGADILLVKPGLPYLDII 360
           SSFYGPFREALDSNPRFGDKKTYQMNPANYREALIETREDE EGADILLVKPGLPYLDII
Sbjct: 301 SSFYGPFREALDSNPRFGDKKTYQMNPANYREALIETREDESEGADILLVKPGLPYLDII 360

Query: 361 RLLRNNSPLPIAAYQVSGEYSMIKAGGVLKMIDEEKVMMESLMCLRRAGADIILTYFALQ 420
           RLLR+NSPLPIAAYQVSGEYSMIKAGGVLKMIDEEKVMMESLMCLRRAGAD+ILTYFALQ
Sbjct: 361 RLLRDNSPLPIAAYQVSGEYSMIKAGGVLKMIDEEKVMMESLMCLRRAGADVILTYFALQ 420

Query: 421 AARCLCGEKR 431
           AARCLCGE+R
Sbjct: 421 AARCLCGERR 430

BLAST of Moc04g31320 vs. NCBI nr
Match: XP_008441165.1 (PREDICTED: delta-aminolevulinic acid dehydratase, chloroplastic [Cucumis melo] >XP_008441166.1 PREDICTED: delta-aminolevulinic acid dehydratase, chloroplastic [Cucumis melo] >XP_008441167.1 PREDICTED: delta-aminolevulinic acid dehydratase, chloroplastic [Cucumis melo] >KAA0037059.1 delta-aminolevulinic acid dehydratase [Cucumis melo var. makuwa] >TYK06590.1 delta-aminolevulinic acid dehydratase [Cucumis melo var. makuwa])

HSP 1 Score: 832.4 bits (2149), Expect = 1.8e-237
Identity = 413/430 (96.05%), Postives = 423/430 (98.37%), Query Frame = 0

Query: 1   MASTVLNAPNCVRTINGLDCGSYVGLKPSPNSNFLCVRTPANVRSPRLFVVRASDERDAH 60
           MASTVLNAPN V+ INGLDCGSYVGLK SPNSNFLCVRT ANVRSPRLFVVRAS+E DAH
Sbjct: 1   MASTVLNAPNSVQRINGLDCGSYVGLKRSPNSNFLCVRTSANVRSPRLFVVRASEEHDAH 60

Query: 61  MKKLGRSDAECEAAVVAGNVPEAPPVPPKPAAPAGTPVAPLLPLSRRPRRNRRSPALRAS 120
           MKKLGRSDAECEAAVVAGN+PEAPPVPPKPA+PAGTPV PLLPLSRRPRRNRRSPA+RAS
Sbjct: 61  MKKLGRSDAECEAAVVAGNIPEAPPVPPKPASPAGTPVVPLLPLSRRPRRNRRSPAMRAS 120

Query: 121 FQETSLSPSNFVYPLFIHEGEEDTPIGAMPGCYRLGWRHGLLEEVSKARDVGVNSVVLFP 180
           FQET+LSPSNFVYPLFIHEGEEDTPIGAMPGCYRLGWRHGLLEEVSKARDVGVNSVVLFP
Sbjct: 121 FQETNLSPSNFVYPLFIHEGEEDTPIGAMPGCYRLGWRHGLLEEVSKARDVGVNSVVLFP 180

Query: 181 KVPDALKSPTGDEAYNDNGLVPRTIRLLKDKYPDLVIYTDVALDPYSSDGHDGIVREDGV 240
           KVPDALK+PTGDEAYNDNGLVPRTIRLLKDKYPDLVIYTDVALDPYSSDGHDGIVREDGV
Sbjct: 181 KVPDALKTPTGDEAYNDNGLVPRTIRLLKDKYPDLVIYTDVALDPYSSDGHDGIVREDGV 240

Query: 241 IMNDETVHQLCKQAVSQARAGADVVSPSDMMDGRVGAIRRALDAEGFHHVSIMSYTAKYA 300
           IMNDETVHQLCKQAVSQARAGADVVSPSDMMDGRVGAIRRALDAEGF+HVSIMSYTAKYA
Sbjct: 241 IMNDETVHQLCKQAVSQARAGADVVSPSDMMDGRVGAIRRALDAEGFYHVSIMSYTAKYA 300

Query: 301 SSFYGPFREALDSNPRFGDKKTYQMNPANYREALIETREDEDEGADILLVKPGLPYLDII 360
           SSFYGPFREALDSNPRFGDKKTYQMNPANYREALIETREDE EGADILLVKPGLPYLDII
Sbjct: 301 SSFYGPFREALDSNPRFGDKKTYQMNPANYREALIETREDESEGADILLVKPGLPYLDII 360

Query: 361 RLLRNNSPLPIAAYQVSGEYSMIKAGGVLKMIDEEKVMMESLMCLRRAGADIILTYFALQ 420
           RLLR+NSPLPIAAYQVSGEYSMIKAGGVLKMIDEEKVMMESLMCLRRAGADIILTYFALQ
Sbjct: 361 RLLRDNSPLPIAAYQVSGEYSMIKAGGVLKMIDEEKVMMESLMCLRRAGADIILTYFALQ 420

Query: 421 AARCLCGEKR 431
           AARCLCGE+R
Sbjct: 421 AARCLCGERR 430

BLAST of Moc04g31320 vs. NCBI nr
Match: XP_004138665.1 (delta-aminolevulinic acid dehydratase, chloroplastic [Cucumis sativus] >XP_031737088.1 delta-aminolevulinic acid dehydratase, chloroplastic [Cucumis sativus] >KGN63283.2 hypothetical protein Csa_022606 [Cucumis sativus])

HSP 1 Score: 826.2 bits (2133), Expect = 1.3e-235
Identity = 412/430 (95.81%), Postives = 422/430 (98.14%), Query Frame = 0

Query: 1   MASTVLNAPNCVRTINGLDCGSYVGLKPSPNSNFLCVRTPANVRSPRLFVVRASDERDAH 60
           MASTVLNAPN V+ INGLD GS VGLK SPNSNFLCVRT ANVRSPRLFVVRAS+ERDAH
Sbjct: 1   MASTVLNAPNSVQRINGLDWGSDVGLKRSPNSNFLCVRTSANVRSPRLFVVRASEERDAH 60

Query: 61  MKKLGRSDAECEAAVVAGNVPEAPPVPPKPAAPAGTPVAPLLPLSRRPRRNRRSPALRAS 120
           MKKLGRSDAECEAAVVAGN+PEAPPVPPKPA+PAGTPV PLLPLSRRPRRNRRSPA+RAS
Sbjct: 61  MKKLGRSDAECEAAVVAGNIPEAPPVPPKPASPAGTPVVPLLPLSRRPRRNRRSPAMRAS 120

Query: 121 FQETSLSPSNFVYPLFIHEGEEDTPIGAMPGCYRLGWRHGLLEEVSKARDVGVNSVVLFP 180
           FQET+LSPSNFVYPLFIHEGEEDTPIGAMPGCYRLGWRHGLLEEVSKARDVGVNSVVLFP
Sbjct: 121 FQETNLSPSNFVYPLFIHEGEEDTPIGAMPGCYRLGWRHGLLEEVSKARDVGVNSVVLFP 180

Query: 181 KVPDALKSPTGDEAYNDNGLVPRTIRLLKDKYPDLVIYTDVALDPYSSDGHDGIVREDGV 240
           KVPDALK+PTGDEAYNDNGLVPRTIRLLKDKYPDLVIYTDVALDPYSSDGHDGIVREDGV
Sbjct: 181 KVPDALKTPTGDEAYNDNGLVPRTIRLLKDKYPDLVIYTDVALDPYSSDGHDGIVREDGV 240

Query: 241 IMNDETVHQLCKQAVSQARAGADVVSPSDMMDGRVGAIRRALDAEGFHHVSIMSYTAKYA 300
           IMNDETVHQLCKQAVSQARAGADVVSPSDMMDGRVGAIRRALDAEGF+HVSIMSYTAKYA
Sbjct: 241 IMNDETVHQLCKQAVSQARAGADVVSPSDMMDGRVGAIRRALDAEGFYHVSIMSYTAKYA 300

Query: 301 SSFYGPFREALDSNPRFGDKKTYQMNPANYREALIETREDEDEGADILLVKPGLPYLDII 360
           SSFYGPFREALDSNPRFGDKKTYQMNPANYREALIETREDE EGADILLVKPGLPYLDII
Sbjct: 301 SSFYGPFREALDSNPRFGDKKTYQMNPANYREALIETREDESEGADILLVKPGLPYLDII 360

Query: 361 RLLRNNSPLPIAAYQVSGEYSMIKAGGVLKMIDEEKVMMESLMCLRRAGADIILTYFALQ 420
           RLLR+NSPLPIAAYQVSGEYSMIKAGGVLKMIDEEKVMMESLMCLRRAGADIILTYFALQ
Sbjct: 361 RLLRDNSPLPIAAYQVSGEYSMIKAGGVLKMIDEEKVMMESLMCLRRAGADIILTYFALQ 420

Query: 421 AARCLCGEKR 431
           AARCLCGE+R
Sbjct: 421 AARCLCGERR 430

BLAST of Moc04g31320 vs. NCBI nr
Match: XP_022955846.1 (delta-aminolevulinic acid dehydratase, chloroplastic [Cucurbita moschata] >KAG7032806.1 Delta-aminolevulinic acid dehydratase 1, chloroplastic [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 819.7 bits (2116), Expect = 1.2e-233
Identity = 409/430 (95.12%), Postives = 419/430 (97.44%), Query Frame = 0

Query: 1   MASTVLNAPNCVRTINGLDCGSYVGLKPSPNSNFLCVRTPANVRSPRLFVVRASDERDAH 60
           MASTVLN P+ VR INGLDCGSYVGL+ SP+SNFLCVRT ANVR PRLFVVRASD  DA 
Sbjct: 1   MASTVLNVPSSVRRINGLDCGSYVGLRHSPDSNFLCVRTSANVRPPRLFVVRASDGGDAP 60

Query: 61  MKKLGRSDAECEAAVVAGNVPEAPPVPPKPAAPAGTPVAPLLPLSRRPRRNRRSPALRAS 120
           MKKLGRSDAECEAAVVAGNVPEAPPVPPKPA+PAGTPV PLLPLSRRPRRNRRSPALRAS
Sbjct: 61  MKKLGRSDAECEAAVVAGNVPEAPPVPPKPASPAGTPVVPLLPLSRRPRRNRRSPALRAS 120

Query: 121 FQETSLSPSNFVYPLFIHEGEEDTPIGAMPGCYRLGWRHGLLEEVSKARDVGVNSVVLFP 180
           FQET+LSP+NFVYPLFIHEGEEDTPIGAMPGCYRLGWRHGLLEEVSKARDVGVNSVVLFP
Sbjct: 121 FQETNLSPANFVYPLFIHEGEEDTPIGAMPGCYRLGWRHGLLEEVSKARDVGVNSVVLFP 180

Query: 181 KVPDALKSPTGDEAYNDNGLVPRTIRLLKDKYPDLVIYTDVALDPYSSDGHDGIVREDGV 240
           KVPDALK+PTGDEAYNDNGLVPRTIRLLKDKYPDLVIYTDVALDPYSSDGHDGIVREDGV
Sbjct: 181 KVPDALKNPTGDEAYNDNGLVPRTIRLLKDKYPDLVIYTDVALDPYSSDGHDGIVREDGV 240

Query: 241 IMNDETVHQLCKQAVSQARAGADVVSPSDMMDGRVGAIRRALDAEGFHHVSIMSYTAKYA 300
           IMNDETVHQLCKQAVSQARAGADVVSPSDMMDGRVGAIRRALDAEGF+HVSIMSYTAKYA
Sbjct: 241 IMNDETVHQLCKQAVSQARAGADVVSPSDMMDGRVGAIRRALDAEGFYHVSIMSYTAKYA 300

Query: 301 SSFYGPFREALDSNPRFGDKKTYQMNPANYREALIETREDEDEGADILLVKPGLPYLDII 360
           SSFYGPFREALDSNPRFGDKKTYQMNPANYREALIETREDE EGADILLVKPGLPYLDII
Sbjct: 301 SSFYGPFREALDSNPRFGDKKTYQMNPANYREALIETREDESEGADILLVKPGLPYLDII 360

Query: 361 RLLRNNSPLPIAAYQVSGEYSMIKAGGVLKMIDEEKVMMESLMCLRRAGADIILTYFALQ 420
           +LLR+NSPLPIAAYQVSGEYSMIKAGGVLKMIDEEKVMMESLMCLRRAGADIILTYFALQ
Sbjct: 361 KLLRDNSPLPIAAYQVSGEYSMIKAGGVLKMIDEEKVMMESLMCLRRAGADIILTYFALQ 420

Query: 421 AARCLCGEKR 431
           AARCLCGEKR
Sbjct: 421 AARCLCGEKR 430

BLAST of Moc04g31320 vs. ExPASy Swiss-Prot
Match: Q9SFH9 (Delta-aminolevulinic acid dehydratase 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=HEMB1 PE=2 SV=1)

HSP 1 Score: 704.9 bits (1818), Expect = 5.6e-202
Identity = 358/431 (83.06%), Postives = 380/431 (88.17%), Query Frame = 0

Query: 1   MAST-VLNAPNCVRTINGLDCGSYVGLKPSPNSNFLCVRTPANVRSPRLFVVRASDERDA 60
           MA+T + NA     +  G+DC SY+GL+ S  S      +       R  VVRAS+  + 
Sbjct: 1   MATTPIFNASCSFPSTRGIDCKSYIGLR-SNVSKVSVASSRIATSQRRNLVVRASESGNG 60

Query: 61  HMKKLGRSDAECEAAVVAGNVPEAPPVPPKPAAPAGTPVAPLLPLSRRPRRNRRSPALRA 120
           H KKLG SDAECEAAV AGNVPEAPPVPPKPAAP GTP+   L LSRRPRRNR SP  RA
Sbjct: 61  HAKKLGMSDAECEAAVAAGNVPEAPPVPPKPAAPVGTPIIKPLNLSRRPRRNRASPVTRA 120

Query: 121 SFQETSLSPSNFVYPLFIHEGEEDTPIGAMPGCYRLGWRHGLLEEVSKARDVGVNSVVLF 180
           +FQET +SP+NFVYPLFIHEGEEDTPIGAMPGCYRLGWRHGL++EV+KAR VGVNS+VLF
Sbjct: 121 AFQETDISPANFVYPLFIHEGEEDTPIGAMPGCYRLGWRHGLVQEVAKARAVGVNSIVLF 180

Query: 181 PKVPDALKSPTGDEAYNDNGLVPRTIRLLKDKYPDLVIYTDVALDPYSSDGHDGIVREDG 240
           PKVP+ALK+ TGDEAYNDNGLVPRTIRLLKDKYPDL+IYTDVALDPYSSDGHDGIVREDG
Sbjct: 181 PKVPEALKNSTGDEAYNDNGLVPRTIRLLKDKYPDLIIYTDVALDPYSSDGHDGIVREDG 240

Query: 241 VIMNDETVHQLCKQAVSQARAGADVVSPSDMMDGRVGAIRRALDAEGFHHVSIMSYTAKY 300
           VIMNDETVHQLCKQAVSQARAGADVVSPSDMMDGRVGAIR ALDAEGF +VSIMSYTAKY
Sbjct: 241 VIMNDETVHQLCKQAVSQARAGADVVSPSDMMDGRVGAIRSALDAEGFQNVSIMSYTAKY 300

Query: 301 ASSFYGPFREALDSNPRFGDKKTYQMNPANYREALIETREDEDEGADILLVKPGLPYLDI 360
           ASSFYGPFREALDSNPRFGDKKTYQMNPANYREALIE REDE EGADILLVKPGLPYLDI
Sbjct: 301 ASSFYGPFREALDSNPRFGDKKTYQMNPANYREALIEAREDEAEGADILLVKPGLPYLDI 360

Query: 361 IRLLRNNSPLPIAAYQVSGEYSMIKAGGVLKMIDEEKVMMESLMCLRRAGADIILTYFAL 420
           IRLLR+ SPLPIAAYQVSGEYSMIKAGGVLKMIDEEKVMMESLMCLRRAGADIILTYFAL
Sbjct: 361 IRLLRDKSPLPIAAYQVSGEYSMIKAGGVLKMIDEEKVMMESLMCLRRAGADIILTYFAL 420

Query: 421 QAARCLCGEKR 431
           QAA CLCGEKR
Sbjct: 421 QAATCLCGEKR 430

BLAST of Moc04g31320 vs. ExPASy Swiss-Prot
Match: P43210 (Delta-aminolevulinic acid dehydratase, chloroplastic OS=Glycine max OX=3847 GN=HEMB PE=2 SV=1)

HSP 1 Score: 690.6 bits (1781), Expect = 1.1e-197
Identity = 355/431 (82.37%), Postives = 377/431 (87.47%), Query Frame = 0

Query: 1   MASTVLNAPNCVRTINGLDCGSYVGLK-PSPNSNFLCVRTPANVRSPRLFVVRASDERDA 60
           MAS++ NAP      +  +  SYVGL+ P    NF   +     RS RLFVVRA      
Sbjct: 1   MASSIPNAP------SAFNSQSYVGLRAPLRTFNFSSPQAAKIPRSQRLFVVRA------ 60

Query: 61  HMKKLGRSDAECEAAVVAGNVPEAPPVPPKPAAPAGTPVAPLLPLSRRPRRNRRSPALRA 120
                  SD+E EAAVVAG VP APPV P+PAAP GTPV P LPL RRPRRNR+SPALR+
Sbjct: 61  -------SDSEFEAAVVAGKVPPAPPVRPRPAAPVGTPVVPSLPLHRRPRRNRKSPALRS 120

Query: 121 SFQETSLSPSNFVYPLFIHEGEEDTPIGAMPGCYRLGWRHGLLEEVSKARDVGVNSVVLF 180
           +FQETS+SP+NFVYPLFIHEGEEDTPIGAMPGCYRLGWRHGL+EEV+KARDVGVNSVVLF
Sbjct: 121 AFQETSISPANFVYPLFIHEGEEDTPIGAMPGCYRLGWRHGLVEEVAKARDVGVNSVVLF 180

Query: 181 PKVPDALKSPTGDEAYNDNGLVPRTIRLLKDKYPDLVIYTDVALDPYSSDGHDGIVREDG 240
           PK+PDALKSPTGDEAYN+NGLVPRTIRLLKDKYPDLVIYTDVALDPYSSDGHDGIVREDG
Sbjct: 181 PKIPDALKSPTGDEAYNENGLVPRTIRLLKDKYPDLVIYTDVALDPYSSDGHDGIVREDG 240

Query: 241 VIMNDETVHQLCKQAVSQARAGADVVSPSDMMDGRVGAIRRALDAEGFHHVSIMSYTAKY 300
           VIMNDETVHQLCKQAV+QA+AGADVVSPSDMMDGRVGA+R ALDAEGF HVSIMSYTAKY
Sbjct: 241 VIMNDETVHQLCKQAVAQAQAGADVVSPSDMMDGRVGALRAALDAEGFQHVSIMSYTAKY 300

Query: 301 ASSFYGPFREALDSNPRFGDKKTYQMNPANYREALIETREDEDEGADILLVKPGLPYLDI 360
           ASSFYGPFREALDSNPRFGDKKTYQMNPANYREAL E REDE EGADILLVKPGLPYLDI
Sbjct: 301 ASSFYGPFREALDSNPRFGDKKTYQMNPANYREALTEMREDESEGADILLVKPGLPYLDI 360

Query: 361 IRLLRNNSPLPIAAYQVSGEYSMIKAGGVLKMIDEEKVMMESLMCLRRAGADIILTYFAL 420
           IRLLR+NSPLPIAAYQVSGEY+MIKA G LKMIDEEKVMMESLMCLRRAGADIILTY AL
Sbjct: 361 IRLLRDNSPLPIAAYQVSGEYAMIKAAGALKMIDEEKVMMESLMCLRRAGADIILTYSAL 412

Query: 421 QAARCLCGEKR 431
           QAARCLCGEKR
Sbjct: 421 QAARCLCGEKR 412

BLAST of Moc04g31320 vs. ExPASy Swiss-Prot
Match: P30124 (Delta-aminolevulinic acid dehydratase, chloroplastic (Fragment) OS=Pisum sativum OX=3888 GN=HEMB PE=1 SV=1)

HSP 1 Score: 682.9 bits (1761), Expect = 2.3e-195
Identity = 345/410 (84.15%), Postives = 369/410 (90.00%), Query Frame = 0

Query: 22  SYVGLK-PSPNSNFLCVRTPANVRSPRLFVVRASDERDAHMKKLGRSDAECEAAVVAGNV 81
           ++V LK P   SN+L   +    + P LF VRA             SD++ EAAVVAG V
Sbjct: 2   TFVDLKSPFTLSNYLSFSSSKRRQPPSLFTVRA-------------SDSDFEAAVVAGKV 61

Query: 82  PEAPPVPPKPAAPAGTPVAPLLPLSRRPRRNRRSPALRASFQETSLSPSNFVYPLFIHEG 141
           PEAPPVPP PA+PAGTPV P LP+ RRPRRNRRSPALR++FQET+LSP+NFVYPLFIHEG
Sbjct: 62  PEAPPVPPTPASPAGTPVVPSLPIQRRPRRNRRSPALRSAFQETTLSPANFVYPLFIHEG 121

Query: 142 EEDTPIGAMPGCYRLGWRHGLLEEVSKARDVGVNSVVLFPKVPDALKSPTGDEAYNDNGL 201
           EEDTPIGAMPGCYRLGWRHGLLEEV+KARDVGVNSVVLFPK+PDALK+PTGDEAYN++GL
Sbjct: 122 EEDTPIGAMPGCYRLGWRHGLLEEVAKARDVGVNSVVLFPKIPDALKTPTGDEAYNEDGL 181

Query: 202 VPRTIRLLKDKYPDLVIYTDVALDPYSSDGHDGIVREDGVIMNDETVHQLCKQAVSQARA 261
           VPR+IRLLKDKYPDL+IYTDVALDPYSSDGHDGIVREDGVIMNDETVHQLCKQAV+QARA
Sbjct: 182 VPRSIRLLKDKYPDLIIYTDVALDPYSSDGHDGIVREDGVIMNDETVHQLCKQAVAQARA 241

Query: 262 GADVVSPSDMMDGRVGAIRRALDAEGFHHVSIMSYTAKYASSFYGPFREALDSNPRFGDK 321
           GADVVSPSDMMDGRVGA+R ALDAEGF HVSIMSYTAKYASSFYGPFREALDSNPRFGDK
Sbjct: 242 GADVVSPSDMMDGRVGAMRVALDAEGFQHVSIMSYTAKYASSFYGPFREALDSNPRFGDK 301

Query: 322 KTYQMNPANYREALIETREDEDEGADILLVKPGLPYLDIIRLLRNNSPLPIAAYQVSGEY 381
           KTYQMNPANYREAL E REDE EGADILLVKPGLPYLDIIRLLR+NSPLPIAAYQVSGEY
Sbjct: 302 KTYQMNPANYREALTEMREDESEGADILLVKPGLPYLDIIRLLRDNSPLPIAAYQVSGEY 361

Query: 382 SMIKAGGVLKMIDEEKVMMESLMCLRRAGADIILTYFALQAARCLCGEKR 431
           SMIKAGG LKMIDEEKVMMESL+CLRRAGADIILTYFALQAAR LCGEKR
Sbjct: 362 SMIKAGGALKMIDEEKVMMESLLCLRRAGADIILTYFALQAARTLCGEKR 398

BLAST of Moc04g31320 vs. ExPASy Swiss-Prot
Match: P24493 (Delta-aminolevulinic acid dehydratase, chloroplastic OS=Spinacia oleracea OX=3562 GN=HEMB PE=1 SV=2)

HSP 1 Score: 652.1 bits (1681), Expect = 4.3e-186
Identity = 329/388 (84.79%), Postives = 351/388 (90.46%), Query Frame = 0

Query: 44  RSPRLFVVRASDERD-AHMKKLGRSDAECEAAVVAGNVPEAPPVPPKPAAPAGTPVAPLL 103
           R P   VVRAS+ RD    +K G S  ECEAAVVAGN P APPVPP P AP+GTP    L
Sbjct: 47  RIPSQLVVRASERRDNLTQQKTGLSIEECEAAVVAGNAPSAPPVPPTPKAPSGTPSVSPL 106

Query: 104 PLSRRPRRNRRSPALRASFQETSLSPSNFVYPLFIHEGEEDTPIGAMPGCYRLGWRHGLL 163
            L RRPRRNR SP  RA+FQET+LSP+N VYPLFIHEGEEDTPIGAMPGCYRLGWRHGL+
Sbjct: 107 SLGRRPRRNRTSPVFRAAFQETTLSPANVVYPLFIHEGEEDTPIGAMPGCYRLGWRHGLV 166

Query: 164 EEVSKARDVGVNSVVLFPKVPDALKSPTGDEAYNDNGLVPRTIRLLKDKYPDLVIYTDVA 223
           EEV+KARDV VNS+V+FPK PDALKSPTGDEAYN+NGLVPRTIR+LKDK+PDL+IYTDVA
Sbjct: 167 EEVAKARDVVVNSIVVFPK-PDALKSPTGDEAYNENGLVPRTIRMLKDKFPDLIIYTDVA 226

Query: 224 LDPYSSDGHDGIVREDGVIMNDETVHQLCKQAVSQARAGADVVSPSDMMDGRVGAIRRAL 283
           LDPY  DGHDGIV + GVIMNDETVHQLCKQAV+QARAGADVVSPSDMMDGRVGAIR AL
Sbjct: 227 LDPYYYDGHDGIVTQHGVIMNDETVHQLCKQAVAQARAGADVVSPSDMMDGRVGAIRAAL 286

Query: 284 DAEGFHHVSIMSYTAKYASSFYGPFREALDSNPRFGDKKTYQMNPANYREALIETREDED 343
           DAEG+ +VSIMSYTAKYASSFYGPFREALDSNPRFGDKKTYQMNPANYREALIET+EDE 
Sbjct: 287 DAEGYSNVSIMSYTAKYASSFYGPFREALDSNPRFGDKKTYQMNPANYREALIETQEDES 346

Query: 344 EGADILLVKPGLPYLDIIRLLRNNSPLPIAAYQVSGEYSMIKAGGVLKMIDEEKVMMESL 403
           EGADILLVKPGLPYLDIIRLLR+NS LPIAAYQVSGEYSMIKAGGVLKMIDEEKVM+ESL
Sbjct: 347 EGADILLVKPGLPYLDIIRLLRDNSDLPIAAYQVSGEYSMIKAGGVLKMIDEEKVMLESL 406

Query: 404 MCLRRAGADIILTYFALQAARCLCGEKR 431
           +CLRRAGADIILTYFALQAARCLCGEKR
Sbjct: 407 LCLRRAGADIILTYFALQAARCLCGEKR 433

BLAST of Moc04g31320 vs. ExPASy Swiss-Prot
Match: Q5Z8V9 (Delta-aminolevulinic acid dehydratase, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=HEMB PE=2 SV=1)

HSP 1 Score: 626.3 bits (1614), Expect = 2.5e-178
Identity = 327/430 (76.05%), Postives = 352/430 (81.86%), Query Frame = 0

Query: 1   MASTVLNAPNCVRTINGLDCGSYVGLKPSPNSNFLCVRTPANVRSPRLFVVRASDERDAH 60
           MASTV  +P  V+ + G  C  +            C   P      R   VR S E++A 
Sbjct: 1   MASTVSFSPANVQMLQGRSCHGHAAFGG-------CSAVPRTGPRMRSVAVRVSSEQEAA 60

Query: 61  ---MKKLGRSDAECEAAVVAGNVPEAPPVPPKPAAPAGTPVAPLLPLSRRPRRNRRSPAL 120
                  GR+  ECEA  VAG  P APP   +P AP GTP    L L++RPRRNRRSPAL
Sbjct: 61  PAVRAPSGRTIEECEADAVAGRFP-APPPLVRPKAPEGTPQIRPLDLTKRPRRNRRSPAL 120

Query: 121 RASFQETSLSPSNFVYPLFIHEGEEDTPIGAMPGCYRLGWRHGLLEEVSKARDVGVNSVV 180
           RA+FQET++SP+N V PLFIHEGE+D PIGAMPGCYRLGWRHGLL+EV K+RDVGVNS V
Sbjct: 121 RAAFQETTISPANLVLPLFIHEGEDDAPIGAMPGCYRLGWRHGLLDEVYKSRDVGVNSFV 180

Query: 181 LFPKVPDALKSPTGDEAYNDNGLVPRTIRLLKDKYPDLVIYTDVALDPYSSDGHDGIVRE 240
           LFPKVPDALKS +GDEAYNDNGLVPRTIRLLKDK+PD+V+YTDVALDPYSSDGHDGIVRE
Sbjct: 181 LFPKVPDALKSQSGDEAYNDNGLVPRTIRLLKDKFPDIVVYTDVALDPYSSDGHDGIVRE 240

Query: 241 DGVIMNDETVHQLCKQAVSQARAGADVVSPSDMMDGRVGAIRRALDAEGFHHVSIMSYTA 300
           DGVIMNDETV+QLCKQAVSQARAGADVVSPSDMMDGRVGAIR ALDAEGFH VSIMSYTA
Sbjct: 241 DGVIMNDETVYQLCKQAVSQARAGADVVSPSDMMDGRVGAIRAALDAEGFHDVSIMSYTA 300

Query: 301 KYASSFYGPFREALDSNPRFGDKKTYQMNPANYREALIETREDEDEGADILLVKPGLPYL 360
           KYASSFYGPFREALDSNPRFGDKKTYQMNPANYREAL+ET  DE EGADILLVKPGLPYL
Sbjct: 301 KYASSFYGPFREALDSNPRFGDKKTYQMNPANYREALLETAADEAEGADILLVKPGLPYL 360

Query: 361 DIIRLLRNNSPLPIAAYQVSGEYSMIKAGGVLKMIDEEKVMMESLMCLRRAGADIILTYF 420
           D+IRLLR+NS LPIAAYQVSGEYSMIKAGG L MIDEEKVMMESLMCLRRAGADIILTYF
Sbjct: 361 DVIRLLRDNSALPIAAYQVSGEYSMIKAGGALNMIDEEKVMMESLMCLRRAGADIILTYF 420

Query: 421 ALQAARCLCG 428
           A QAA  LCG
Sbjct: 421 ARQAANVLCG 422

BLAST of Moc04g31320 vs. ExPASy TrEMBL
Match: A0A6J1CKY2 (Delta-aminolevulinic acid dehydratase OS=Momordica charantia OX=3673 GN=LOC111011917 PE=3 SV=1)

HSP 1 Score: 864.0 bits (2231), Expect = 2.7e-247
Identity = 430/430 (100.00%), Postives = 430/430 (100.00%), Query Frame = 0

Query: 1   MASTVLNAPNCVRTINGLDCGSYVGLKPSPNSNFLCVRTPANVRSPRLFVVRASDERDAH 60
           MASTVLNAPNCVRTINGLDCGSYVGLKPSPNSNFLCVRTPANVRSPRLFVVRASDERDAH
Sbjct: 1   MASTVLNAPNCVRTINGLDCGSYVGLKPSPNSNFLCVRTPANVRSPRLFVVRASDERDAH 60

Query: 61  MKKLGRSDAECEAAVVAGNVPEAPPVPPKPAAPAGTPVAPLLPLSRRPRRNRRSPALRAS 120
           MKKLGRSDAECEAAVVAGNVPEAPPVPPKPAAPAGTPVAPLLPLSRRPRRNRRSPALRAS
Sbjct: 61  MKKLGRSDAECEAAVVAGNVPEAPPVPPKPAAPAGTPVAPLLPLSRRPRRNRRSPALRAS 120

Query: 121 FQETSLSPSNFVYPLFIHEGEEDTPIGAMPGCYRLGWRHGLLEEVSKARDVGVNSVVLFP 180
           FQETSLSPSNFVYPLFIHEGEEDTPIGAMPGCYRLGWRHGLLEEVSKARDVGVNSVVLFP
Sbjct: 121 FQETSLSPSNFVYPLFIHEGEEDTPIGAMPGCYRLGWRHGLLEEVSKARDVGVNSVVLFP 180

Query: 181 KVPDALKSPTGDEAYNDNGLVPRTIRLLKDKYPDLVIYTDVALDPYSSDGHDGIVREDGV 240
           KVPDALKSPTGDEAYNDNGLVPRTIRLLKDKYPDLVIYTDVALDPYSSDGHDGIVREDGV
Sbjct: 181 KVPDALKSPTGDEAYNDNGLVPRTIRLLKDKYPDLVIYTDVALDPYSSDGHDGIVREDGV 240

Query: 241 IMNDETVHQLCKQAVSQARAGADVVSPSDMMDGRVGAIRRALDAEGFHHVSIMSYTAKYA 300
           IMNDETVHQLCKQAVSQARAGADVVSPSDMMDGRVGAIRRALDAEGFHHVSIMSYTAKYA
Sbjct: 241 IMNDETVHQLCKQAVSQARAGADVVSPSDMMDGRVGAIRRALDAEGFHHVSIMSYTAKYA 300

Query: 301 SSFYGPFREALDSNPRFGDKKTYQMNPANYREALIETREDEDEGADILLVKPGLPYLDII 360
           SSFYGPFREALDSNPRFGDKKTYQMNPANYREALIETREDEDEGADILLVKPGLPYLDII
Sbjct: 301 SSFYGPFREALDSNPRFGDKKTYQMNPANYREALIETREDEDEGADILLVKPGLPYLDII 360

Query: 361 RLLRNNSPLPIAAYQVSGEYSMIKAGGVLKMIDEEKVMMESLMCLRRAGADIILTYFALQ 420
           RLLRNNSPLPIAAYQVSGEYSMIKAGGVLKMIDEEKVMMESLMCLRRAGADIILTYFALQ
Sbjct: 361 RLLRNNSPLPIAAYQVSGEYSMIKAGGVLKMIDEEKVMMESLMCLRRAGADIILTYFALQ 420

Query: 421 AARCLCGEKR 431
           AARCLCGEKR
Sbjct: 421 AARCLCGEKR 430

BLAST of Moc04g31320 vs. ExPASy TrEMBL
Match: A0A1S3B2C1 (Delta-aminolevulinic acid dehydratase OS=Cucumis melo OX=3656 GN=LOC103485385 PE=3 SV=1)

HSP 1 Score: 832.4 bits (2149), Expect = 8.6e-238
Identity = 413/430 (96.05%), Postives = 423/430 (98.37%), Query Frame = 0

Query: 1   MASTVLNAPNCVRTINGLDCGSYVGLKPSPNSNFLCVRTPANVRSPRLFVVRASDERDAH 60
           MASTVLNAPN V+ INGLDCGSYVGLK SPNSNFLCVRT ANVRSPRLFVVRAS+E DAH
Sbjct: 1   MASTVLNAPNSVQRINGLDCGSYVGLKRSPNSNFLCVRTSANVRSPRLFVVRASEEHDAH 60

Query: 61  MKKLGRSDAECEAAVVAGNVPEAPPVPPKPAAPAGTPVAPLLPLSRRPRRNRRSPALRAS 120
           MKKLGRSDAECEAAVVAGN+PEAPPVPPKPA+PAGTPV PLLPLSRRPRRNRRSPA+RAS
Sbjct: 61  MKKLGRSDAECEAAVVAGNIPEAPPVPPKPASPAGTPVVPLLPLSRRPRRNRRSPAMRAS 120

Query: 121 FQETSLSPSNFVYPLFIHEGEEDTPIGAMPGCYRLGWRHGLLEEVSKARDVGVNSVVLFP 180
           FQET+LSPSNFVYPLFIHEGEEDTPIGAMPGCYRLGWRHGLLEEVSKARDVGVNSVVLFP
Sbjct: 121 FQETNLSPSNFVYPLFIHEGEEDTPIGAMPGCYRLGWRHGLLEEVSKARDVGVNSVVLFP 180

Query: 181 KVPDALKSPTGDEAYNDNGLVPRTIRLLKDKYPDLVIYTDVALDPYSSDGHDGIVREDGV 240
           KVPDALK+PTGDEAYNDNGLVPRTIRLLKDKYPDLVIYTDVALDPYSSDGHDGIVREDGV
Sbjct: 181 KVPDALKTPTGDEAYNDNGLVPRTIRLLKDKYPDLVIYTDVALDPYSSDGHDGIVREDGV 240

Query: 241 IMNDETVHQLCKQAVSQARAGADVVSPSDMMDGRVGAIRRALDAEGFHHVSIMSYTAKYA 300
           IMNDETVHQLCKQAVSQARAGADVVSPSDMMDGRVGAIRRALDAEGF+HVSIMSYTAKYA
Sbjct: 241 IMNDETVHQLCKQAVSQARAGADVVSPSDMMDGRVGAIRRALDAEGFYHVSIMSYTAKYA 300

Query: 301 SSFYGPFREALDSNPRFGDKKTYQMNPANYREALIETREDEDEGADILLVKPGLPYLDII 360
           SSFYGPFREALDSNPRFGDKKTYQMNPANYREALIETREDE EGADILLVKPGLPYLDII
Sbjct: 301 SSFYGPFREALDSNPRFGDKKTYQMNPANYREALIETREDESEGADILLVKPGLPYLDII 360

Query: 361 RLLRNNSPLPIAAYQVSGEYSMIKAGGVLKMIDEEKVMMESLMCLRRAGADIILTYFALQ 420
           RLLR+NSPLPIAAYQVSGEYSMIKAGGVLKMIDEEKVMMESLMCLRRAGADIILTYFALQ
Sbjct: 361 RLLRDNSPLPIAAYQVSGEYSMIKAGGVLKMIDEEKVMMESLMCLRRAGADIILTYFALQ 420

Query: 421 AARCLCGEKR 431
           AARCLCGE+R
Sbjct: 421 AARCLCGERR 430

BLAST of Moc04g31320 vs. ExPASy TrEMBL
Match: A0A5D3C5H5 (Delta-aminolevulinic acid dehydratase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold453G00580 PE=3 SV=1)

HSP 1 Score: 832.4 bits (2149), Expect = 8.6e-238
Identity = 413/430 (96.05%), Postives = 423/430 (98.37%), Query Frame = 0

Query: 1   MASTVLNAPNCVRTINGLDCGSYVGLKPSPNSNFLCVRTPANVRSPRLFVVRASDERDAH 60
           MASTVLNAPN V+ INGLDCGSYVGLK SPNSNFLCVRT ANVRSPRLFVVRAS+E DAH
Sbjct: 1   MASTVLNAPNSVQRINGLDCGSYVGLKRSPNSNFLCVRTSANVRSPRLFVVRASEEHDAH 60

Query: 61  MKKLGRSDAECEAAVVAGNVPEAPPVPPKPAAPAGTPVAPLLPLSRRPRRNRRSPALRAS 120
           MKKLGRSDAECEAAVVAGN+PEAPPVPPKPA+PAGTPV PLLPLSRRPRRNRRSPA+RAS
Sbjct: 61  MKKLGRSDAECEAAVVAGNIPEAPPVPPKPASPAGTPVVPLLPLSRRPRRNRRSPAMRAS 120

Query: 121 FQETSLSPSNFVYPLFIHEGEEDTPIGAMPGCYRLGWRHGLLEEVSKARDVGVNSVVLFP 180
           FQET+LSPSNFVYPLFIHEGEEDTPIGAMPGCYRLGWRHGLLEEVSKARDVGVNSVVLFP
Sbjct: 121 FQETNLSPSNFVYPLFIHEGEEDTPIGAMPGCYRLGWRHGLLEEVSKARDVGVNSVVLFP 180

Query: 181 KVPDALKSPTGDEAYNDNGLVPRTIRLLKDKYPDLVIYTDVALDPYSSDGHDGIVREDGV 240
           KVPDALK+PTGDEAYNDNGLVPRTIRLLKDKYPDLVIYTDVALDPYSSDGHDGIVREDGV
Sbjct: 181 KVPDALKTPTGDEAYNDNGLVPRTIRLLKDKYPDLVIYTDVALDPYSSDGHDGIVREDGV 240

Query: 241 IMNDETVHQLCKQAVSQARAGADVVSPSDMMDGRVGAIRRALDAEGFHHVSIMSYTAKYA 300
           IMNDETVHQLCKQAVSQARAGADVVSPSDMMDGRVGAIRRALDAEGF+HVSIMSYTAKYA
Sbjct: 241 IMNDETVHQLCKQAVSQARAGADVVSPSDMMDGRVGAIRRALDAEGFYHVSIMSYTAKYA 300

Query: 301 SSFYGPFREALDSNPRFGDKKTYQMNPANYREALIETREDEDEGADILLVKPGLPYLDII 360
           SSFYGPFREALDSNPRFGDKKTYQMNPANYREALIETREDE EGADILLVKPGLPYLDII
Sbjct: 301 SSFYGPFREALDSNPRFGDKKTYQMNPANYREALIETREDESEGADILLVKPGLPYLDII 360

Query: 361 RLLRNNSPLPIAAYQVSGEYSMIKAGGVLKMIDEEKVMMESLMCLRRAGADIILTYFALQ 420
           RLLR+NSPLPIAAYQVSGEYSMIKAGGVLKMIDEEKVMMESLMCLRRAGADIILTYFALQ
Sbjct: 361 RLLRDNSPLPIAAYQVSGEYSMIKAGGVLKMIDEEKVMMESLMCLRRAGADIILTYFALQ 420

Query: 421 AARCLCGEKR 431
           AARCLCGE+R
Sbjct: 421 AARCLCGERR 430

BLAST of Moc04g31320 vs. ExPASy TrEMBL
Match: A0A6J1GUZ1 (Delta-aminolevulinic acid dehydratase OS=Cucurbita moschata OX=3662 GN=LOC111457708 PE=3 SV=1)

HSP 1 Score: 819.7 bits (2116), Expect = 5.7e-234
Identity = 409/430 (95.12%), Postives = 419/430 (97.44%), Query Frame = 0

Query: 1   MASTVLNAPNCVRTINGLDCGSYVGLKPSPNSNFLCVRTPANVRSPRLFVVRASDERDAH 60
           MASTVLN P+ VR INGLDCGSYVGL+ SP+SNFLCVRT ANVR PRLFVVRASD  DA 
Sbjct: 1   MASTVLNVPSSVRRINGLDCGSYVGLRHSPDSNFLCVRTSANVRPPRLFVVRASDGGDAP 60

Query: 61  MKKLGRSDAECEAAVVAGNVPEAPPVPPKPAAPAGTPVAPLLPLSRRPRRNRRSPALRAS 120
           MKKLGRSDAECEAAVVAGNVPEAPPVPPKPA+PAGTPV PLLPLSRRPRRNRRSPALRAS
Sbjct: 61  MKKLGRSDAECEAAVVAGNVPEAPPVPPKPASPAGTPVVPLLPLSRRPRRNRRSPALRAS 120

Query: 121 FQETSLSPSNFVYPLFIHEGEEDTPIGAMPGCYRLGWRHGLLEEVSKARDVGVNSVVLFP 180
           FQET+LSP+NFVYPLFIHEGEEDTPIGAMPGCYRLGWRHGLLEEVSKARDVGVNSVVLFP
Sbjct: 121 FQETNLSPANFVYPLFIHEGEEDTPIGAMPGCYRLGWRHGLLEEVSKARDVGVNSVVLFP 180

Query: 181 KVPDALKSPTGDEAYNDNGLVPRTIRLLKDKYPDLVIYTDVALDPYSSDGHDGIVREDGV 240
           KVPDALK+PTGDEAYNDNGLVPRTIRLLKDKYPDLVIYTDVALDPYSSDGHDGIVREDGV
Sbjct: 181 KVPDALKNPTGDEAYNDNGLVPRTIRLLKDKYPDLVIYTDVALDPYSSDGHDGIVREDGV 240

Query: 241 IMNDETVHQLCKQAVSQARAGADVVSPSDMMDGRVGAIRRALDAEGFHHVSIMSYTAKYA 300
           IMNDETVHQLCKQAVSQARAGADVVSPSDMMDGRVGAIRRALDAEGF+HVSIMSYTAKYA
Sbjct: 241 IMNDETVHQLCKQAVSQARAGADVVSPSDMMDGRVGAIRRALDAEGFYHVSIMSYTAKYA 300

Query: 301 SSFYGPFREALDSNPRFGDKKTYQMNPANYREALIETREDEDEGADILLVKPGLPYLDII 360
           SSFYGPFREALDSNPRFGDKKTYQMNPANYREALIETREDE EGADILLVKPGLPYLDII
Sbjct: 301 SSFYGPFREALDSNPRFGDKKTYQMNPANYREALIETREDESEGADILLVKPGLPYLDII 360

Query: 361 RLLRNNSPLPIAAYQVSGEYSMIKAGGVLKMIDEEKVMMESLMCLRRAGADIILTYFALQ 420
           +LLR+NSPLPIAAYQVSGEYSMIKAGGVLKMIDEEKVMMESLMCLRRAGADIILTYFALQ
Sbjct: 361 KLLRDNSPLPIAAYQVSGEYSMIKAGGVLKMIDEEKVMMESLMCLRRAGADIILTYFALQ 420

Query: 421 AARCLCGEKR 431
           AARCLCGEKR
Sbjct: 421 AARCLCGEKR 430

BLAST of Moc04g31320 vs. ExPASy TrEMBL
Match: A0A6J1JQZ9 (Delta-aminolevulinic acid dehydratase OS=Cucurbita maxima OX=3661 GN=LOC111487051 PE=3 SV=1)

HSP 1 Score: 814.7 bits (2103), Expect = 1.8e-232
Identity = 408/430 (94.88%), Postives = 418/430 (97.21%), Query Frame = 0

Query: 1   MASTVLNAPNCVRTINGLDCGSYVGLKPSPNSNFLCVRTPANVRSPRLFVVRASDERDAH 60
           MASTVLN PN VR I GLDCGSYVGL+ SP+SNFLCVRT ANVRSPRLFVVRASD  DA 
Sbjct: 1   MASTVLNVPNSVRRITGLDCGSYVGLRHSPDSNFLCVRTSANVRSPRLFVVRASDGGDAP 60

Query: 61  MKKLGRSDAECEAAVVAGNVPEAPPVPPKPAAPAGTPVAPLLPLSRRPRRNRRSPALRAS 120
           MKKLGRSDAECEAAVVAGNVPEAPPV PKPA+PAGTPV PLLPLSRRPRRNRRSPALRAS
Sbjct: 61  MKKLGRSDAECEAAVVAGNVPEAPPVTPKPASPAGTPVVPLLPLSRRPRRNRRSPALRAS 120

Query: 121 FQETSLSPSNFVYPLFIHEGEEDTPIGAMPGCYRLGWRHGLLEEVSKARDVGVNSVVLFP 180
           FQET+LSP+NFVYPLFIHEGEEDTPIGAMPGCYRLGWRHGLLEEVSKARDVGVNSVVLFP
Sbjct: 121 FQETNLSPANFVYPLFIHEGEEDTPIGAMPGCYRLGWRHGLLEEVSKARDVGVNSVVLFP 180

Query: 181 KVPDALKSPTGDEAYNDNGLVPRTIRLLKDKYPDLVIYTDVALDPYSSDGHDGIVREDGV 240
           KVPDALK+PTGDEAYND+GLVPRTIRLLKDKYPDLVIYTDVALDPYSSDGHDGIVREDGV
Sbjct: 181 KVPDALKNPTGDEAYNDDGLVPRTIRLLKDKYPDLVIYTDVALDPYSSDGHDGIVREDGV 240

Query: 241 IMNDETVHQLCKQAVSQARAGADVVSPSDMMDGRVGAIRRALDAEGFHHVSIMSYTAKYA 300
           IMNDETVHQLCKQAVSQARAGADVVSPSDMMDGRVGAIRRALDAEGF+HVSIMSYTAKYA
Sbjct: 241 IMNDETVHQLCKQAVSQARAGADVVSPSDMMDGRVGAIRRALDAEGFYHVSIMSYTAKYA 300

Query: 301 SSFYGPFREALDSNPRFGDKKTYQMNPANYREALIETREDEDEGADILLVKPGLPYLDII 360
           SSFYGPFREALDSNPRFGDKKTYQMNPANYREALIETREDE EGADILLVKPGLPYLDII
Sbjct: 301 SSFYGPFREALDSNPRFGDKKTYQMNPANYREALIETREDEAEGADILLVKPGLPYLDII 360

Query: 361 RLLRNNSPLPIAAYQVSGEYSMIKAGGVLKMIDEEKVMMESLMCLRRAGADIILTYFALQ 420
           RLLR++SPLPIAAYQVSGEYSMIKAGGVLKMIDEEKVMMESLMCLRRAGADIILTYFALQ
Sbjct: 361 RLLRDSSPLPIAAYQVSGEYSMIKAGGVLKMIDEEKVMMESLMCLRRAGADIILTYFALQ 420

Query: 421 AARCLCGEKR 431
           AARCLCGEKR
Sbjct: 421 AARCLCGEKR 430

BLAST of Moc04g31320 vs. TAIR 10
Match: AT1G69740.1 (Aldolase superfamily protein )

HSP 1 Score: 704.9 bits (1818), Expect = 4.0e-203
Identity = 358/431 (83.06%), Postives = 380/431 (88.17%), Query Frame = 0

Query: 1   MAST-VLNAPNCVRTINGLDCGSYVGLKPSPNSNFLCVRTPANVRSPRLFVVRASDERDA 60
           MA+T + NA     +  G+DC SY+GL+ S  S      +       R  VVRAS+  + 
Sbjct: 1   MATTPIFNASCSFPSTRGIDCKSYIGLR-SNVSKVSVASSRIATSQRRNLVVRASESGNG 60

Query: 61  HMKKLGRSDAECEAAVVAGNVPEAPPVPPKPAAPAGTPVAPLLPLSRRPRRNRRSPALRA 120
           H KKLG SDAECEAAV AGNVPEAPPVPPKPAAP GTP+   L LSRRPRRNR SP  RA
Sbjct: 61  HAKKLGMSDAECEAAVAAGNVPEAPPVPPKPAAPVGTPIIKPLNLSRRPRRNRASPVTRA 120

Query: 121 SFQETSLSPSNFVYPLFIHEGEEDTPIGAMPGCYRLGWRHGLLEEVSKARDVGVNSVVLF 180
           +FQET +SP+NFVYPLFIHEGEEDTPIGAMPGCYRLGWRHGL++EV+KAR VGVNS+VLF
Sbjct: 121 AFQETDISPANFVYPLFIHEGEEDTPIGAMPGCYRLGWRHGLVQEVAKARAVGVNSIVLF 180

Query: 181 PKVPDALKSPTGDEAYNDNGLVPRTIRLLKDKYPDLVIYTDVALDPYSSDGHDGIVREDG 240
           PKVP+ALK+ TGDEAYNDNGLVPRTIRLLKDKYPDL+IYTDVALDPYSSDGHDGIVREDG
Sbjct: 181 PKVPEALKNSTGDEAYNDNGLVPRTIRLLKDKYPDLIIYTDVALDPYSSDGHDGIVREDG 240

Query: 241 VIMNDETVHQLCKQAVSQARAGADVVSPSDMMDGRVGAIRRALDAEGFHHVSIMSYTAKY 300
           VIMNDETVHQLCKQAVSQARAGADVVSPSDMMDGRVGAIR ALDAEGF +VSIMSYTAKY
Sbjct: 241 VIMNDETVHQLCKQAVSQARAGADVVSPSDMMDGRVGAIRSALDAEGFQNVSIMSYTAKY 300

Query: 301 ASSFYGPFREALDSNPRFGDKKTYQMNPANYREALIETREDEDEGADILLVKPGLPYLDI 360
           ASSFYGPFREALDSNPRFGDKKTYQMNPANYREALIE REDE EGADILLVKPGLPYLDI
Sbjct: 301 ASSFYGPFREALDSNPRFGDKKTYQMNPANYREALIEAREDEAEGADILLVKPGLPYLDI 360

Query: 361 IRLLRNNSPLPIAAYQVSGEYSMIKAGGVLKMIDEEKVMMESLMCLRRAGADIILTYFAL 420
           IRLLR+ SPLPIAAYQVSGEYSMIKAGGVLKMIDEEKVMMESLMCLRRAGADIILTYFAL
Sbjct: 361 IRLLRDKSPLPIAAYQVSGEYSMIKAGGVLKMIDEEKVMMESLMCLRRAGADIILTYFAL 420

Query: 421 QAARCLCGEKR 431
           QAA CLCGEKR
Sbjct: 421 QAATCLCGEKR 430

BLAST of Moc04g31320 vs. TAIR 10
Match: AT1G69740.2 (Aldolase superfamily protein )

HSP 1 Score: 704.9 bits (1818), Expect = 4.0e-203
Identity = 358/431 (83.06%), Postives = 380/431 (88.17%), Query Frame = 0

Query: 1   MAST-VLNAPNCVRTINGLDCGSYVGLKPSPNSNFLCVRTPANVRSPRLFVVRASDERDA 60
           MA+T + NA     +  G+DC SY+GL+ S  S      +       R  VVRAS+  + 
Sbjct: 1   MATTPIFNASCSFPSTRGIDCKSYIGLR-SNVSKVSVASSRIATSQRRNLVVRASESGNG 60

Query: 61  HMKKLGRSDAECEAAVVAGNVPEAPPVPPKPAAPAGTPVAPLLPLSRRPRRNRRSPALRA 120
           H KKLG SDAECEAAV AGNVPEAPPVPPKPAAP GTP+   L LSRRPRRNR SP  RA
Sbjct: 61  HAKKLGMSDAECEAAVAAGNVPEAPPVPPKPAAPVGTPIIKPLNLSRRPRRNRASPVTRA 120

Query: 121 SFQETSLSPSNFVYPLFIHEGEEDTPIGAMPGCYRLGWRHGLLEEVSKARDVGVNSVVLF 180
           +FQET +SP+NFVYPLFIHEGEEDTPIGAMPGCYRLGWRHGL++EV+KAR VGVNS+VLF
Sbjct: 121 AFQETDISPANFVYPLFIHEGEEDTPIGAMPGCYRLGWRHGLVQEVAKARAVGVNSIVLF 180

Query: 181 PKVPDALKSPTGDEAYNDNGLVPRTIRLLKDKYPDLVIYTDVALDPYSSDGHDGIVREDG 240
           PKVP+ALK+ TGDEAYNDNGLVPRTIRLLKDKYPDL+IYTDVALDPYSSDGHDGIVREDG
Sbjct: 181 PKVPEALKNSTGDEAYNDNGLVPRTIRLLKDKYPDLIIYTDVALDPYSSDGHDGIVREDG 240

Query: 241 VIMNDETVHQLCKQAVSQARAGADVVSPSDMMDGRVGAIRRALDAEGFHHVSIMSYTAKY 300
           VIMNDETVHQLCKQAVSQARAGADVVSPSDMMDGRVGAIR ALDAEGF +VSIMSYTAKY
Sbjct: 241 VIMNDETVHQLCKQAVSQARAGADVVSPSDMMDGRVGAIRSALDAEGFQNVSIMSYTAKY 300

Query: 301 ASSFYGPFREALDSNPRFGDKKTYQMNPANYREALIETREDEDEGADILLVKPGLPYLDI 360
           ASSFYGPFREALDSNPRFGDKKTYQMNPANYREALIE REDE EGADILLVKPGLPYLDI
Sbjct: 301 ASSFYGPFREALDSNPRFGDKKTYQMNPANYREALIEAREDEAEGADILLVKPGLPYLDI 360

Query: 361 IRLLRNNSPLPIAAYQVSGEYSMIKAGGVLKMIDEEKVMMESLMCLRRAGADIILTYFAL 420
           IRLLR+ SPLPIAAYQVSGEYSMIKAGGVLKMIDEEKVMMESLMCLRRAGADIILTYFAL
Sbjct: 361 IRLLRDKSPLPIAAYQVSGEYSMIKAGGVLKMIDEEKVMMESLMCLRRAGADIILTYFAL 420

Query: 421 QAARCLCGEKR 431
           QAA CLCGEKR
Sbjct: 421 QAATCLCGEKR 430

BLAST of Moc04g31320 vs. TAIR 10
Match: AT1G44318.1 (Aldolase superfamily protein )

HSP 1 Score: 503.1 bits (1294), Expect = 2.3e-142
Identity = 270/430 (62.79%), Postives = 320/430 (74.42%), Query Frame = 0

Query: 1   MASTVLNAPNCVRTINGLDCGSYVGLKPSPNSNFLCVRTPANVRSPRLFVVRASDERDAH 60
           M S++  +P  + ++ G +  SYVGLK +            NVR         S E  + 
Sbjct: 1   MTSSMFRSPCKIPSVKGFEQKSYVGLKAA----------SYNVRVNSF----KSSEVASQ 60

Query: 61  MKKLGRSDAECEAAVVAGNVPEAPPVPPKPAAPAGTPVAPLLPLSRRPRRNRRSPALRAS 120
           ++K+          +   N  EAPPVP KPA P    +   L LSRR RRNR+ P  RA+
Sbjct: 61  LQKID------STLIWPVNALEAPPVPSKPATPL---IDQPLQLSRRARRNRKCPTQRAA 120

Query: 121 FQETSLSPSNFVYPLFIHEGEEDTPIGAMPGCYRLGWRHGLLEEVSKARDVGVNSVVLFP 180
           FQET++SP+NF+YPLFIHEGE D PI +MPG Y LGWRHGL+EEV++A DVGVNSV L+P
Sbjct: 121 FQETNISPANFIYPLFIHEGEVDIPITSMPGRYMLGWRHGLIEEVARALDVGVNSVKLYP 180

Query: 181 KVPDALKSPTGDEAYNDNGLVPRTIRLLKDKYPDLVIYTDVALDPYSSDGHDGIVREDGV 240
           KVP+ALKSPTG+EA+NDNGL+PRT+RLLKD++PDLVIYTDV  D YS+ GH GIV EDGV
Sbjct: 181 KVPEALKSPTGEEAFNDNGLIPRTVRLLKDRFPDLVIYTDVNFDEYSTTGHGGIVGEDGV 240

Query: 241 IMNDETVHQLCKQAVSQARAGADVVSPSDMMDGRVGAIRRALDAEGFHHVSIMSYTAKYA 300
           I+NDET+HQL KQAVSQARAGADVV  S+M+DGRVGA+R ALDAEGF  VSIMSY+ KY 
Sbjct: 241 ILNDETIHQLRKQAVSQARAGADVVCTSEMLDGRVGAVRAALDAEGFQDVSIMSYSVKYT 300

Query: 301 SSFYGPFREALDSNPRFGDKKTYQMNPANYREALIETREDEDEGADILLVKPGLPYLDII 360
           SS YG FR+         DKKTYQ+NPAN REAL+E REDE EGADIL+VKP LP LDII
Sbjct: 301 SSLYGRFRKVQ------LDKKTYQINPANSREALLEAREDEAEGADILMVKPALPSLDII 360

Query: 361 RLLRNNSPLPIAAYQVSGEYSMIKAGGVLKMIDEEKVMMESLMCLRRAGADIILTYFALQ 420
           RLL+N + LPI A QVSGEYSMIKA G+LKMIDEEKVMMESL+C+RRAGAD+ILTYFALQ
Sbjct: 361 RLLKNQTLLPIGACQVSGEYSMIKAAGLLKMIDEEKVMMESLLCIRRAGADLILTYFALQ 401

Query: 421 AARCLCGEKR 431
           AA  LCGE +
Sbjct: 421 AATKLCGENK 401

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022141587.15.5e-247100.00delta-aminolevulinic acid dehydratase, chloroplastic [Momordica charantia] >XP_0... [more]
XP_038886111.13.6e-23896.05delta-aminolevulinic acid dehydratase, chloroplastic [Benincasa hispida][more]
XP_008441165.11.8e-23796.05PREDICTED: delta-aminolevulinic acid dehydratase, chloroplastic [Cucumis melo] >... [more]
XP_004138665.11.3e-23595.81delta-aminolevulinic acid dehydratase, chloroplastic [Cucumis sativus] >XP_03173... [more]
XP_022955846.11.2e-23395.12delta-aminolevulinic acid dehydratase, chloroplastic [Cucurbita moschata] >KAG70... [more]
Match NameE-valueIdentityDescription
Q9SFH95.6e-20283.06Delta-aminolevulinic acid dehydratase 1, chloroplastic OS=Arabidopsis thaliana O... [more]
P432101.1e-19782.37Delta-aminolevulinic acid dehydratase, chloroplastic OS=Glycine max OX=3847 GN=H... [more]
P301242.3e-19584.15Delta-aminolevulinic acid dehydratase, chloroplastic (Fragment) OS=Pisum sativum... [more]
P244934.3e-18684.79Delta-aminolevulinic acid dehydratase, chloroplastic OS=Spinacia oleracea OX=356... [more]
Q5Z8V92.5e-17876.05Delta-aminolevulinic acid dehydratase, chloroplastic OS=Oryza sativa subsp. japo... [more]
Match NameE-valueIdentityDescription
A0A6J1CKY22.7e-247100.00Delta-aminolevulinic acid dehydratase OS=Momordica charantia OX=3673 GN=LOC11101... [more]
A0A1S3B2C18.6e-23896.05Delta-aminolevulinic acid dehydratase OS=Cucumis melo OX=3656 GN=LOC103485385 PE... [more]
A0A5D3C5H58.6e-23896.05Delta-aminolevulinic acid dehydratase OS=Cucumis melo var. makuwa OX=1194695 GN=... [more]
A0A6J1GUZ15.7e-23495.12Delta-aminolevulinic acid dehydratase OS=Cucurbita moschata OX=3662 GN=LOC111457... [more]
A0A6J1JQZ91.8e-23294.88Delta-aminolevulinic acid dehydratase OS=Cucurbita maxima OX=3661 GN=LOC11148705... [more]
Match NameE-valueIdentityDescription
AT1G69740.14.0e-20383.06Aldolase superfamily protein [more]
AT1G69740.24.0e-20383.06Aldolase superfamily protein [more]
AT1G44318.12.3e-14262.79Aldolase superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (OHB3-1) v2
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001731Delta-aminolevulinic acid dehydratasePRINTSPR00144DALDHYDRTASEcoord: 344..360
score: 71.92
coord: 399..418
score: 62.74
coord: 292..311
score: 75.32
coord: 223..237
score: 52.47
coord: 254..273
score: 73.39
coord: 369..384
score: 74.8
IPR001731Delta-aminolevulinic acid dehydrataseSMARTSM01004ALAD_2coord: 103..425
e-value: 2.4E-213
score: 724.7
IPR001731Delta-aminolevulinic acid dehydratasePFAMPF00490ALADcoord: 106..423
e-value: 8.4E-134
score: 445.7
IPR001731Delta-aminolevulinic acid dehydratasePANTHERPTHR11458DELTA-AMINOLEVULINIC ACID DEHYDRATASEcoord: 80..429
IPR013785Aldolase-type TIM barrelGENE3D3.20.20.70Aldolase class Icoord: 96..430
e-value: 2.8E-154
score: 515.3
NoneNo IPR availablePIRSRPIRSR001415-1PIRSR001415-1coord: 106..424
e-value: 4.3E-133
score: 441.0
NoneNo IPR availablePIRSRPIRSR001415-5PIRSR001415-5coord: 106..423
e-value: 7.4E-122
score: 404.2
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 81..118
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 81..97
NoneNo IPR availablePANTHERPTHR11458:SF3DELTA-AMINOLEVULINIC ACID DEHYDRATASE 2, CHLOROPLASTIC-RELATEDcoord: 80..429
NoneNo IPR availableCDDcd04823ALAD_PBGS_aspartate_richcoord: 116..426
e-value: 0.0
score: 586.832
NoneNo IPR availableSUPERFAMILY51569Aldolasecoord: 101..426
IPR030656Delta-aminolevulinic acid dehydratase, active sitePROSITEPS00169D_ALA_DEHYDRATASEcoord: 344..356

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Moc04g31320.1Moc04g31320.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0015995 chlorophyll biosynthetic process
biological_process GO:0006782 protoporphyrinogen IX biosynthetic process
biological_process GO:0033014 tetrapyrrole biosynthetic process
molecular_function GO:0046872 metal ion binding
molecular_function GO:0004655 porphobilinogen synthase activity
molecular_function GO:0003824 catalytic activity