Moc04g29480 (gene) Bitter gourd (OHB3-1) v2

Overview
NameMoc04g29480
Typegene
OrganismMomordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
DescriptionNAD(P)-binding Rossmann-fold superfamily protein
Locationchr4: 21881442 .. 21883289 (+)
RNA-Seq ExpressionMoc04g29480
SyntenyMoc04g29480
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAGTCACATGCAAACTGTCGTTTTCTGCCTTTTAGACACTTCCACTTCCATTGCCAAATATATCGAGATAAGAGAGAGAAACAGAGCAACAATGGCTACACCTTCTTCTGCTGAATCTTCTTCTCTCCCAACCCAAAGGTATTTCCCTTTCCTTTCCTAATCTTTCTTCTTTATTCTCTCTGTTCAAATTTTGAGGTCCCATGGATCTATCTTTGTTCTTCTTCTGTACTTTACTCATAGATTTATTCTGACCCTTTTGAGAATTTTGAAGCTGTGATGCAAATTCCTCTCCTTTTCTTCCCTAAATTGCATGCTCTGTTTCAAACATGGGATGAATCTTACTGCTGTTGATTGAACTCACTTGTTACGGAAAAGTTCTTATTCTAATAAGCTGTTACTGTTGAGGGTGAAATGTTGATTGGATTAACGCGACGGGAAAGGAAAGAACAAACGAAAGGGAGAATCAGAATGTTTATGAAACTTTCCCTTTCTCAAAGTGTTTTTTTCTTCCCTTTTTTAAGCCCATTTTTCAACCGTTCTTCTCATTTTCATTAAAGTGGTGAACATTTATGAATAACAATCCAAGTAGTATGAGAAATACTAATAAAAATTCGCTTTACTGTTTTTGTTCTTATAATAGTCCTTGTTTTTTATCCAAAAAATTTCAACTTTTCGCTGCTATTTTTGGATTTCCCCCCCCCCCCCCCAAGAAATATGATTTTTGTGAGGCGCCATACGATTTTAGCTTTACGATCGGCTTTCGAGTTCCCGAATGGAGTAGATTATACTGTTTAAGATGGGGAAACAATTGTCATTTCTGGAAAATGTTCTTACCAACATGTTTTTGCTCTATGATTTAGCTCTTGGGGCTAAACTAAGGATTTAACTGGGTTTCAAGTGTGCCATCGACTAGCTTTTTATTCATCGTTATCGTGATTCGGGATGATATATATTGTGTTCTTTTAGATTTGGTATGGGAATGTGCAAGTATTTCAGAATCAAACGTTGTAGAATTTGATATGAAGTATTGATTCTTACCATTTTCAGGTTGTTGGGGAAAGTGGCATTGGTCACCGGAGGAGCCTCTGGCATTGGAGAGGCCATTGTGCGGCTCTTCCACAGACATGGTGCAAAAGTTTGCTTTGTCGATGTGCAGGACGAGCTTGGTCAGCATCTCAACGAAAGCCTCGGCGACAATGCGAACACTTGTTACTTCCATTGTGATGTCACCATGGAAGACGACATACGTCGTGCAGTAGACTCGACAGTGAGTAAATTCGGCACGCTTGACATAATGGTGAACAATGCTGGGATCTCAGGCACACCCTGCTCCGATATCCGCAACGTCAATGTGTTGGAGTTCGAGAGGGTGTTTGATATAAACGTGAAGGCAGTTTTCGTGGGAATGAAGTATGCTGCCAATGTAATGATTCCACGCAAACAAGGATCGATCATATCTCTAGCGAGTGTTGGTAGCGTGATGGCGGGAGTAGGGCCTCATCACTACGTAGGCTCCAAGCACGCTGTGCTCGGGCTTACGAGGAGCGTTGCAGCCGAGCTGGGACAACACGGGATTCGTGTAAATTGTGTCTCCCCTTACGCAGTTCCAACGGCCTTGGCTGTAGCTCACTTGCCCGAAGATGAGAGAACTGAGGATGTGTTTGTTGGTTTTCGCGAGTTTGCTGGAAAGATTGCTAATCTGCAGGGAGTTCAGCTCGAGGTCGAAGATGTTGCCAACGCCGTGCTGTTCTTAGCAAGCGACGAGGCTAGGTACATCAGCGGAGACAATCTCCTCGTCGATGGTGGCTTCACTCGAGTAAATCACGCGCTTCGTGTTTTCAGATGA

mRNA sequence

ATGAGTCACATGCAAACTGTCGTTTTCTGCCTTTTAGACACTTCCACTTCCATTGCCAAATATATCGAGATAAGAGAGAGAAACAGAGCAACAATGGCTACACCTTCTTCTGCTGAATCTTCTTCTCTCCCAACCCAAAGGTTGTTGGGGAAAGTGGCATTGGTCACCGGAGGAGCCTCTGGCATTGGAGAGGCCATTGTGCGGCTCTTCCACAGACATGGTGCAAAAGTTTGCTTTGTCGATGTGCAGGACGAGCTTGGTCAGCATCTCAACGAAAGCCTCGGCGACAATGCGAACACTTGTTACTTCCATTGTGATGTCACCATGGAAGACGACATACGTCGTGCAGTAGACTCGACAGTGAGTAAATTCGGCACGCTTGACATAATGGTGAACAATGCTGGGATCTCAGGCACACCCTGCTCCGATATCCGCAACGTCAATGTGTTGGAGTTCGAGAGGGTGTTTGATATAAACGTGAAGGCAGTTTTCGTGGGAATGAAGTATGCTGCCAATGTAATGATTCCACGCAAACAAGGATCGATCATATCTCTAGCGAGTGTTGGTAGCGTGATGGCGGGAGTAGGGCCTCATCACTACGTAGGCTCCAAGCACGCTGTGCTCGGGCTTACGAGGAGCGTTGCAGCCGAGCTGGGACAACACGGGATTCGTGTAAATTGTGTCTCCCCTTACGCAGTTCCAACGGCCTTGGCTGTAGCTCACTTGCCCGAAGATGAGAGAACTGAGGATGTGTTTGTTGGTTTTCGCGAGTTTGCTGGAAAGATTGCTAATCTGCAGGGAGTTCAGCTCGAGGTCGAAGATGTTGCCAACGCCGTGCTGTTCTTAGCAAGCGACGAGGCTAGGTACATCAGCGGAGACAATCTCCTCGTCGATGGTGGCTTCACTCGAGTAAATCACGCGCTTCGTGTTTTCAGATGA

Coding sequence (CDS)

ATGAGTCACATGCAAACTGTCGTTTTCTGCCTTTTAGACACTTCCACTTCCATTGCCAAATATATCGAGATAAGAGAGAGAAACAGAGCAACAATGGCTACACCTTCTTCTGCTGAATCTTCTTCTCTCCCAACCCAAAGGTTGTTGGGGAAAGTGGCATTGGTCACCGGAGGAGCCTCTGGCATTGGAGAGGCCATTGTGCGGCTCTTCCACAGACATGGTGCAAAAGTTTGCTTTGTCGATGTGCAGGACGAGCTTGGTCAGCATCTCAACGAAAGCCTCGGCGACAATGCGAACACTTGTTACTTCCATTGTGATGTCACCATGGAAGACGACATACGTCGTGCAGTAGACTCGACAGTGAGTAAATTCGGCACGCTTGACATAATGGTGAACAATGCTGGGATCTCAGGCACACCCTGCTCCGATATCCGCAACGTCAATGTGTTGGAGTTCGAGAGGGTGTTTGATATAAACGTGAAGGCAGTTTTCGTGGGAATGAAGTATGCTGCCAATGTAATGATTCCACGCAAACAAGGATCGATCATATCTCTAGCGAGTGTTGGTAGCGTGATGGCGGGAGTAGGGCCTCATCACTACGTAGGCTCCAAGCACGCTGTGCTCGGGCTTACGAGGAGCGTTGCAGCCGAGCTGGGACAACACGGGATTCGTGTAAATTGTGTCTCCCCTTACGCAGTTCCAACGGCCTTGGCTGTAGCTCACTTGCCCGAAGATGAGAGAACTGAGGATGTGTTTGTTGGTTTTCGCGAGTTTGCTGGAAAGATTGCTAATCTGCAGGGAGTTCAGCTCGAGGTCGAAGATGTTGCCAACGCCGTGCTGTTCTTAGCAAGCGACGAGGCTAGGTACATCAGCGGAGACAATCTCCTCGTCGATGGTGGCTTCACTCGAGTAAATCACGCGCTTCGTGTTTTCAGATGA

Protein sequence

MSHMQTVVFCLLDTSTSIAKYIEIRERNRATMATPSSAESSSLPTQRLLGKVALVTGGASGIGEAIVRLFHRHGAKVCFVDVQDELGQHLNESLGDNANTCYFHCDVTMEDDIRRAVDSTVSKFGTLDIMVNNAGISGTPCSDIRNVNVLEFERVFDINVKAVFVGMKYAANVMIPRKQGSIISLASVGSVMAGVGPHHYVGSKHAVLGLTRSVAAELGQHGIRVNCVSPYAVPTALAVAHLPEDERTEDVFVGFREFAGKIANLQGVQLEVEDVANAVLFLASDEARYISGDNLLVDGGFTRVNHALRVFR
Homology
BLAST of Moc04g29480 vs. NCBI nr
Match: XP_022157898.1 (xanthoxin dehydrogenase [Momordica charantia])

HSP 1 Score: 614.8 bits (1584), Expect = 4.2e-172
Identity = 312/312 (100.00%), Postives = 312/312 (100.00%), Query Frame = 0

Query: 1   MSHMQTVVFCLLDTSTSIAKYIEIRERNRATMATPSSAESSSLPTQRLLGKVALVTGGAS 60
           MSHMQTVVFCLLDTSTSIAKYIEIRERNRATMATPSSAESSSLPTQRLLGKVALVTGGAS
Sbjct: 1   MSHMQTVVFCLLDTSTSIAKYIEIRERNRATMATPSSAESSSLPTQRLLGKVALVTGGAS 60

Query: 61  GIGEAIVRLFHRHGAKVCFVDVQDELGQHLNESLGDNANTCYFHCDVTMEDDIRRAVDST 120
           GIGEAIVRLFHRHGAKVCFVDVQDELGQHLNESLGDNANTCYFHCDVTMEDDIRRAVDST
Sbjct: 61  GIGEAIVRLFHRHGAKVCFVDVQDELGQHLNESLGDNANTCYFHCDVTMEDDIRRAVDST 120

Query: 121 VSKFGTLDIMVNNAGISGTPCSDIRNVNVLEFERVFDINVKAVFVGMKYAANVMIPRKQG 180
           VSKFGTLDIMVNNAGISGTPCSDIRNVNVLEFERVFDINVKAVFVGMKYAANVMIPRKQG
Sbjct: 121 VSKFGTLDIMVNNAGISGTPCSDIRNVNVLEFERVFDINVKAVFVGMKYAANVMIPRKQG 180

Query: 181 SIISLASVGSVMAGVGPHHYVGSKHAVLGLTRSVAAELGQHGIRVNCVSPYAVPTALAVA 240
           SIISLASVGSVMAGVGPHHYVGSKHAVLGLTRSVAAELGQHGIRVNCVSPYAVPTALAVA
Sbjct: 181 SIISLASVGSVMAGVGPHHYVGSKHAVLGLTRSVAAELGQHGIRVNCVSPYAVPTALAVA 240

Query: 241 HLPEDERTEDVFVGFREFAGKIANLQGVQLEVEDVANAVLFLASDEARYISGDNLLVDGG 300
           HLPEDERTEDVFVGFREFAGKIANLQGVQLEVEDVANAVLFLASDEARYISGDNLLVDGG
Sbjct: 241 HLPEDERTEDVFVGFREFAGKIANLQGVQLEVEDVANAVLFLASDEARYISGDNLLVDGG 300

Query: 301 FTRVNHALRVFR 313
           FTRVNHALRVFR
Sbjct: 301 FTRVNHALRVFR 312

BLAST of Moc04g29480 vs. NCBI nr
Match: XP_038906598.1 (xanthoxin dehydrogenase [Benincasa hispida])

HSP 1 Score: 469.2 bits (1206), Expect = 2.9e-128
Identity = 239/279 (85.66%), Postives = 258/279 (92.47%), Query Frame = 0

Query: 36  SSAESSSLPTQRLLGKVALVTGGASGIGEAIVRLFHRHGAKVCFVDVQDELGQHLNESLG 95
           ++ +SSSL TQRLLGKVALVTGGASGIGEAIVRLFHRHGAKVCFVDVQDE G  L+E+LG
Sbjct: 2   ATLQSSSLITQRLLGKVALVTGGASGIGEAIVRLFHRHGAKVCFVDVQDEPGYRLHENLG 61

Query: 96  --DNANTCYFHCDVTMEDDIRRAVDSTVSKFGTLDIMVNNAGISGTPCSDIRNVNVLEFE 155
              ++NT Y HCDVT+E+DIRRAVD TVSKFGTLDIMVNNAGISGTP SDIRNV+V EFE
Sbjct: 62  GDKDSNTYYSHCDVTVEEDIRRAVDLTVSKFGTLDIMVNNAGISGTPSSDIRNVDVSEFE 121

Query: 156 RVFDINVKAVFVGMKYAANVMIPRKQGSIISLASVGSVMAGVGPHHYVGSKHAVLGLTRS 215
           RVFDINVKAVF+GMKYAA+VMIPRKQGSIISL SVGSV+ G+GPHHYVGSKHAVLGLTRS
Sbjct: 122 RVFDINVKAVFMGMKYAASVMIPRKQGSIISLGSVGSVIGGIGPHHYVGSKHAVLGLTRS 181

Query: 216 VAAELGQHGIRVNCVSPYAVPTALAVAHLPEDERTEDVFVGFREFAGKIANLQGVQLEVE 275
           VAAELGQHGIRVNCVSPY VPT L+VAHLPE+ERTEDVF  FREFA K ANLQGV+LEVE
Sbjct: 182 VAAELGQHGIRVNCVSPYGVPTGLSVAHLPEEERTEDVFTDFREFAKKNANLQGVELEVE 241

Query: 276 DVANAVLFLASDEARYISGDNLLVDGGFTRVNHALRVFR 313
           DVANAVLFLASDEARYISGDNLLVDGGFTRVNH+LRVF+
Sbjct: 242 DVANAVLFLASDEARYISGDNLLVDGGFTRVNHSLRVFK 280

BLAST of Moc04g29480 vs. NCBI nr
Match: XP_023549777.1 (xanthoxin dehydrogenase [Cucurbita pepo subsp. pepo])

HSP 1 Score: 461.1 bits (1185), Expect = 7.8e-126
Identity = 235/283 (83.04%), Postives = 259/283 (91.52%), Query Frame = 0

Query: 32  MATPSSAESSSLPTQRLLGKVALVTGGASGIGEAIVRLFHRHGAKVCFVDVQDELGQHLN 91
           MAT  S+ SS +P QRLLGKVALVTGGASGIGEAIVRLFHRHGAKVCFVDVQDELG+ L+
Sbjct: 1   MATAESSSSSLVP-QRLLGKVALVTGGASGIGEAIVRLFHRHGAKVCFVDVQDELGRRLH 60

Query: 92  ESLG--DNANTCYFHCDVTMEDDIRRAVDSTVSKFGTLDIMVNNAGISGTPCSDIRNVNV 151
           E LG  D++NT Y HCDVT+EDD+RRAVD+TVSKFGTLDIMVNNAGISGTP SDIRNV+V
Sbjct: 61  EGLGGNDDSNTFYSHCDVTVEDDVRRAVDTTVSKFGTLDIMVNNAGISGTPSSDIRNVDV 120

Query: 152 LEFERVFDINVKAVFVGMKYAANVMIPRKQGSIISLASVGSVMAGVGPHHYVGSKHAVLG 211
            EFE+VFDINVKAVF+GMK+AA+VMIP KQGSIISLAS+GSV+ G+GPHHY+ SKHAVLG
Sbjct: 121 SEFEKVFDINVKAVFMGMKHAASVMIPNKQGSIISLASIGSVIGGIGPHHYISSKHAVLG 180

Query: 212 LTRSVAAELGQHGIRVNCVSPYAVPTALAVAHLPEDERTEDVFVGFREFAGKIANLQGVQ 271
           LTRSVAAELGQ+GIRVNCVSPYAVPT+LA AHL E+ERTEDV  GFREF GK ANLQGV 
Sbjct: 181 LTRSVAAELGQYGIRVNCVSPYAVPTSLAFAHLHEEERTEDVITGFREFTGKNANLQGVD 240

Query: 272 LEVEDVANAVLFLASDEARYISGDNLLVDGGFTRVNHALRVFR 313
           LEVEDVANAVLFLAS+EARYISGDNLLVDGGF+ VNH+LRVFR
Sbjct: 241 LEVEDVANAVLFLASNEARYISGDNLLVDGGFSCVNHSLRVFR 282

BLAST of Moc04g29480 vs. NCBI nr
Match: XP_008465699.1 (PREDICTED: xanthoxin dehydrogenase [Cucumis melo] >TYJ99851.1 xanthoxin dehydrogenase [Cucumis melo var. makuwa])

HSP 1 Score: 461.1 bits (1185), Expect = 7.8e-126
Identity = 237/283 (83.75%), Postives = 255/283 (90.11%), Query Frame = 0

Query: 32  MATPSSAESSSLPTQRLLGKVALVTGGASGIGEAIVRLFHRHGAKVCFVDVQDELGQHLN 91
           MATP     SSL +QRLLGKVA VTGGASGIGE IVRLFHRHGAKVCFVDVQDELG  L 
Sbjct: 1   MATP---PISSLVSQRLLGKVAFVTGGASGIGEGIVRLFHRHGAKVCFVDVQDELGYRLL 60

Query: 92  ESLG--DNANTCYFHCDVTMEDDIRRAVDSTVSKFGTLDIMVNNAGISGTPCSDIRNVNV 151
           ESLG   + N  Y HCDVT+EDD+RRAVD TV++FGTLDIMVNNAGISGTP SDIRNV++
Sbjct: 61  ESLGGDKDPNIFYSHCDVTVEDDLRRAVDLTVTRFGTLDIMVNNAGISGTPSSDIRNVDI 120

Query: 152 LEFERVFDINVKAVFVGMKYAANVMIPRKQGSIISLASVGSVMAGVGPHHYVGSKHAVLG 211
            EFE+VFDINVKAVF+GMKYAA+VMIPRKQGSIISL SVGSV+ G+GPHHYVGSKHAV+G
Sbjct: 121 SEFEKVFDINVKAVFMGMKYAASVMIPRKQGSIISLGSVGSVIGGIGPHHYVGSKHAVVG 180

Query: 212 LTRSVAAELGQHGIRVNCVSPYAVPTALAVAHLPEDERTEDVFVGFREFAGKIANLQGVQ 271
           LTRSVAAELGQHGIRVNCVSPYAVPT LAVAHLPEDERTEDVF GFREFA K ANLQGV+
Sbjct: 181 LTRSVAAELGQHGIRVNCVSPYAVPTNLAVAHLPEDERTEDVFTGFREFAKKNANLQGVE 240

Query: 272 LEVEDVANAVLFLASDEARYISGDNLLVDGGFTRVNHALRVFR 313
           L VEDVANAVLFLAS++ARYISGDNLLVDGGFTRVNH+LRVFR
Sbjct: 241 LTVEDVANAVLFLASEDARYISGDNLLVDGGFTRVNHSLRVFR 280

BLAST of Moc04g29480 vs. NCBI nr
Match: KAG6579113.1 (Xanthoxin dehydrogenase, partial [Cucurbita argyrosperma subsp. sororia] >KAG7016637.1 Xanthoxin dehydrogenase [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 458.0 bits (1177), Expect = 6.6e-125
Identity = 234/283 (82.69%), Postives = 257/283 (90.81%), Query Frame = 0

Query: 32  MATPSSAESSSLPTQRLLGKVALVTGGASGIGEAIVRLFHRHGAKVCFVDVQDELGQHLN 91
           MAT  S+ SS +P QRLLGKVALVTGGASGIGEAIVRLFHRHGAKVCFVDVQDELG  L+
Sbjct: 1   MATAESSSSSLVP-QRLLGKVALVTGGASGIGEAIVRLFHRHGAKVCFVDVQDELGHRLH 60

Query: 92  ESLG--DNANTCYFHCDVTMEDDIRRAVDSTVSKFGTLDIMVNNAGISGTPCSDIRNVNV 151
           E LG  D++NT Y HCDVT+EDD+RRAVD+TVSKFGTLDIMVNNAGISGTP SDIRNV+V
Sbjct: 61  EDLGGNDDSNTFYSHCDVTVEDDVRRAVDTTVSKFGTLDIMVNNAGISGTPTSDIRNVDV 120

Query: 152 LEFERVFDINVKAVFVGMKYAANVMIPRKQGSIISLASVGSVMAGVGPHHYVGSKHAVLG 211
            EFE+VFDINVKAVF+GMK+AA+VMIP KQGSIISLAS+GSV+ G+GPHHY  SKHAVLG
Sbjct: 121 SEFEKVFDINVKAVFMGMKHAASVMIPNKQGSIISLASIGSVIGGIGPHHYTSSKHAVLG 180

Query: 212 LTRSVAAELGQHGIRVNCVSPYAVPTALAVAHLPEDERTEDVFVGFREFAGKIANLQGVQ 271
           LTRSVAAELGQ+GIRVNCVSPYAVPT+LA AHL E+ERTEDV  GFR+F GK ANLQGV 
Sbjct: 181 LTRSVAAELGQYGIRVNCVSPYAVPTSLAFAHLHEEERTEDVITGFRQFIGKNANLQGVD 240

Query: 272 LEVEDVANAVLFLASDEARYISGDNLLVDGGFTRVNHALRVFR 313
           LEVEDVANAVLFLAS+EARYISGDNLLVDGGF+ VNH+LRVFR
Sbjct: 241 LEVEDVANAVLFLASNEARYISGDNLLVDGGFSCVNHSLRVFR 282

BLAST of Moc04g29480 vs. ExPASy Swiss-Prot
Match: Q9C826 (Xanthoxin dehydrogenase OS=Arabidopsis thaliana OX=3702 GN=ABA2 PE=1 SV=1)

HSP 1 Score: 396.4 bits (1017), Expect = 3.1e-109
Identity = 196/279 (70.25%), Postives = 232/279 (83.15%), Query Frame = 0

Query: 37  SAESSSLPTQRLLGKVALVTGGASGIGEAIVRLFHRHGAKVCFVDVQDELGQHLNESL-- 96
           S+  SSLP+QRLLGKVAL+TGGA+GIGE+IVRLFH+HGAKVC VD+QD+LG  + +SL  
Sbjct: 7   SSSYSSLPSQRLLGKVALITGGATGIGESIVRLFHKHGAKVCIVDLQDDLGGEVCKSLLR 66

Query: 97  GDNANTCYF-HCDVTMEDDIRRAVDSTVSKFGTLDIMVNNAGISGTPCSDIRNVNVLEFE 156
           G++  T +F H DV +EDDI  AVD  V  FGTLDI++NNAG+ G PC DIRN ++ EFE
Sbjct: 67  GESKETAFFIHGDVRVEDDISNAVDFAVKNFGTLDILINNAGLCGAPCPDIRNYSLSEFE 126

Query: 157 RVFDINVKAVFVGMKYAANVMIPRKQGSIISLASVGSVMAGVGPHHYVGSKHAVLGLTRS 216
             FD+NVK  F+ MK+AA VMIP K+GSI+SL SVG V+ GVGPH YVGSKHAVLGLTRS
Sbjct: 127 MTFDVNVKGAFLSMKHAARVMIPEKKGSIVSLCSVGGVVGGVGPHSYVGSKHAVLGLTRS 186

Query: 217 VAAELGQHGIRVNCVSPYAVPTALAVAHLPEDERTEDVFVGFREFAGKIANLQGVQLEVE 276
           VAAELGQHGIRVNCVSPYAV T LA+AHLPE+ERTED FVGFR FA   ANL+GV+L V+
Sbjct: 187 VAAELGQHGIRVNCVSPYAVATKLALAHLPEEERTEDAFVGFRNFAAANANLKGVELTVD 246

Query: 277 DVANAVLFLASDEARYISGDNLLVDGGFTRVNHALRVFR 313
           DVANAVLFLASD++RYISGDNL++DGGFT  NH+ +VFR
Sbjct: 247 DVANAVLFLASDDSRYISGDNLMIDGGFTCTNHSFKVFR 285

BLAST of Moc04g29480 vs. ExPASy Swiss-Prot
Match: F1SWA0 (Zerumbone synthase OS=Zingiber zerumbet OX=311405 GN=ZSD1 PE=1 SV=1)

HSP 1 Score: 359.0 bits (920), Expect = 5.4e-98
Identity = 173/265 (65.28%), Postives = 214/265 (80.75%), Query Frame = 0

Query: 47  RLLGKVALVTGGASGIGEAIVRLFHRHGAKVCFVDVQDELGQHLNESLGDNANTCYFHCD 106
           RL GKVALVTGGASGIGE+I RLF  HGAK+C VDVQDELGQ +++ LG + + CYFHCD
Sbjct: 2   RLEGKVALVTGGASGIGESIARLFIEHGAKICIVDVQDELGQQVSQRLGGDPHACYFHCD 61

Query: 107 VTMEDDIRRAVDSTVSKFGTLDIMVNNAGISGTPCSDIRNVNVLEFERVFDINVKAVFVG 166
           VT+EDD+RRAVD T  K+GT+DIMVNNAGI+G    DIR+ +  EF++VFDINV  VF+G
Sbjct: 62  VTVEDDVRRAVDFTAEKYGTIDIMVNNAGITGDKVIDIRDADFNEFKKVFDINVNGVFLG 121

Query: 167 MKYAANVMIPRKQGSIISLASVGSVMAGVGPHHYVGSKHAVLGLTRSVAAELGQHGIRVN 226
           MK+AA +MIP+ +GSI+SLASV SV+AG GPH Y G+KHAV+GLT+SVAAELG+HGIRVN
Sbjct: 122 MKHAARIMIPKMKGSIVSLASVSSVIAGAGPHGYTGAKHAVVGLTKSVAAELGRHGIRVN 181

Query: 227 CVSPYAVPTALAVAHLPEDERTEDVFVGFREFAGKIANLQGVQLEVEDVANAVLFLASDE 286
           CVSPYAVPT L++ +LPE E  ED   GF  F    ANL+GV L   DVA AVL+LA++E
Sbjct: 182 CVSPYAVPTRLSMPYLPESEMQEDALRGFLTFVRSNANLKGVDLMPNDVAEAVLYLATEE 241

Query: 287 ARYISGDNLLVDGGFTRVNHALRVF 312
           ++Y+SG NL++DGGF+  NH L+VF
Sbjct: 242 SKYVSGLNLVIDGGFSIANHTLQVF 266

BLAST of Moc04g29480 vs. ExPASy Swiss-Prot
Match: Q94KL7 (Secoisolariciresinol dehydrogenase (Fragment) OS=Forsythia intermedia OX=55183 PE=1 SV=1)

HSP 1 Score: 280.0 bits (715), Expect = 3.2e-74
Identity = 148/267 (55.43%), Postives = 187/267 (70.04%), Query Frame = 0

Query: 46  QRLLGKVALVTGGASGIGEAIVRLFHRHGAKVCFVDVQDELGQHLNESLGDNANTCYFHC 105
           +RL GKVAL+TGGASGIGE   +LF +HGAKV   DVQDELG  + E++G  +N+ Y HC
Sbjct: 13  RRLEGKVALITGGASGIGETTAKLFSQHGAKVAIADVQDELGHSVVEAIG-TSNSTYIHC 72

Query: 106 DVTMEDDIRRAVDSTVSKFGTLDIMVNNAGISGTPCSDIRNVNVLEFERVFDINVKAVFV 165
           DVT ED ++ AVD+TVS +G LDIM +NAGIS      I +    +FERVF +NV  VF+
Sbjct: 73  DVTNEDGVKNAVDNTVSTYGKLDIMFSNAGISDPNRPRIIDNEKADFERVFSVNVTGVFL 132

Query: 166 GMKYAANVMIPRKQGSIISLASVGSVMAGVGPHHYVGSKHAVLGLTRSVAAELGQHGIRV 225
            MK+AA VMIP + G+IIS AS+ S M G   H Y GSKHAVLGLTR++A ELGQ GIRV
Sbjct: 133 CMKHAARVMIPARSGNIISTASLSSTMGGGSSHAYCGSKHAVLGLTRNLAVELGQFGIRV 192

Query: 226 NCVSPYAVPTALAVAHLPEDERTEDVFVGFREFAGKIANLQGVQLEVEDVANAVLFLASD 285
           NC+SP+ +PTAL         + E+ F     FAG   NL+G +  VEDVANA L+LASD
Sbjct: 193 NCLSPFGLPTALGKKF--SGIKNEEEFENVINFAG---NLKGPKFNVEDVANAALYLASD 252

Query: 286 EARYISGDNLLVDGGFTRVNHALRVFR 313
           EA+Y+SG NL +DGGF+  N  ++VF+
Sbjct: 253 EAKYVSGHNLFIDGGFSVCNSVIKVFQ 273

BLAST of Moc04g29480 vs. ExPASy Swiss-Prot
Match: Q7FAE1 (Momilactone A synthase OS=Oryza sativa subsp. japonica OX=39947 GN=Os04g0179200 PE=2 SV=1)

HSP 1 Score: 252.7 bits (644), Expect = 5.5e-66
Identity = 138/282 (48.94%), Postives = 182/282 (64.54%), Query Frame = 0

Query: 32  MATPSSAESSSLPTQRLLGKVALVTGGASGIGEAIVRLFHRHGAKVCFVDVQDELGQHLN 91
           MA  SS  S+    ++L+GKVA++TGGASGIG    RLF +HGA+V   D+QDELG  L 
Sbjct: 1   MAAGSSHVSAD--ARKLVGKVAVITGGASGIGACTARLFVKHGARVVVADIQDELGASLV 60

Query: 92  ESLGDNANTCYFHCDVTMEDDIRRAVDSTVSKFGTLDIMVNNAGISGTPCSDIRNVNVLE 151
             LG +A++ Y HCDVT E D+  AVD  V++FG LD+M NNAG+SG PC  +      +
Sbjct: 61  AELGPDASS-YVHCDVTNEGDVAAAVDHAVARFGKLDVMFNNAGVSGPPCFRMSECTKED 120

Query: 152 FERVFDINVKAVFVGMKYAANVMIPRKQGSIISLASVGSVMAGVGPHHYVGSKHAVLGLT 211
           FERV  +N+   F+G K+AA VM P ++GSIIS AS+ S ++G   H Y  SKHA++G T
Sbjct: 121 FERVLAVNLVGPFLGTKHAARVMAPARRGSIISTASLSSSVSGAASHAYTTSKHALVGFT 180

Query: 212 RSVAAELGQHGIRVNCVSPYAVPTALAVAHLPEDERTEDVFVGFREFAGKIANLQGV-QL 271
            + A ELG+HGIRVNCVSP  V T LA A +  D+   +  +         ANL+G   L
Sbjct: 181 ENAAGELGRHGIRVNCVSPAGVATPLARAAMGMDDEAIEAIM------ANSANLKGAGAL 240

Query: 272 EVEDVANAVLFLASDEARYISGDNLLVDGGFTRVNHALRVFR 313
           + +D+A A LFLASD+ RY+SG NL VDGG + VN +   FR
Sbjct: 241 KADDIAAAALFLASDDGRYVSGQNLRVDGGLSVVNSSFGFFR 273

BLAST of Moc04g29480 vs. ExPASy Swiss-Prot
Match: A3F5F0 (Secoisolariciresinol dehydrogenase OS=Sinopodophyllum hexandrum OX=93608 GN=SDH PE=1 SV=1)

HSP 1 Score: 244.2 bits (622), Expect = 2.0e-63
Identity = 130/272 (47.79%), Postives = 171/272 (62.87%), Query Frame = 0

Query: 37  SAESSSLPTQRLLGKVALVTGGASGIGEAIVRLFHRHGAKVCFVDVQDELGQHLNESLGD 96
           S  + +  T RL  KVA++TGGA GIGE   +LF R+GAKV   D+ D+ GQ +  ++G 
Sbjct: 3   STSTPASSTNRLQDKVAIITGGAGGIGETTAKLFVRYGAKVVIADISDDHGQKVCNNIGS 62

Query: 97  NANTCYFHCDVTMEDDIRRAVDSTVSKFGTLDIMVNNAGISGTPCSDIRNVNVLEFERVF 156
                + HCDVT ++D+R  VD+T++K G LDIM  N G+  T    I      +F+RV 
Sbjct: 63  PDVISFVHCDVTKDEDVRNLVDTTIAKHGKLDIMFGNVGVLSTTPYSILEAGNEDFKRVM 122

Query: 157 DINVKAVFVGMKYAANVMIPRKQGSIISLASVGSVMAGVGPHH-YVGSKHAVLGLTRSVA 216
           DINV   F+  K+AA VMIP K+GSI+  AS+ S  AG G  H Y  +KHAVLGLT S+ 
Sbjct: 123 DINVYGAFLVAKHAARVMIPAKKGSIVFTASISSFTAGEGVSHVYTATKHAVLGLTTSLC 182

Query: 217 AELGQHGIRVNCVSPYAVPTALAVAHLPEDERTEDVFVGFREFAGKIANLQGVQLEVEDV 276
            ELGQHGIRVNCVSPY V + L       D    +      E A + ANL+G+ L  EDV
Sbjct: 183 TELGQHGIRVNCVSPYVVASPLLTDVFGVDSSRVE------ELAHQAANLKGILLRAEDV 242

Query: 277 ANAVLFLASDEARYISGDNLLVDGGFTRVNHA 308
           A+AV +LA DE++Y+SG NL++DGG+TR N A
Sbjct: 243 ADAVAYLAGDESKYVSGLNLVIDGGYTRTNPA 268

BLAST of Moc04g29480 vs. ExPASy TrEMBL
Match: A0A6J1DUL7 (xanthoxin dehydrogenase OS=Momordica charantia OX=3673 GN=LOC111024504 PE=4 SV=1)

HSP 1 Score: 614.8 bits (1584), Expect = 2.0e-172
Identity = 312/312 (100.00%), Postives = 312/312 (100.00%), Query Frame = 0

Query: 1   MSHMQTVVFCLLDTSTSIAKYIEIRERNRATMATPSSAESSSLPTQRLLGKVALVTGGAS 60
           MSHMQTVVFCLLDTSTSIAKYIEIRERNRATMATPSSAESSSLPTQRLLGKVALVTGGAS
Sbjct: 1   MSHMQTVVFCLLDTSTSIAKYIEIRERNRATMATPSSAESSSLPTQRLLGKVALVTGGAS 60

Query: 61  GIGEAIVRLFHRHGAKVCFVDVQDELGQHLNESLGDNANTCYFHCDVTMEDDIRRAVDST 120
           GIGEAIVRLFHRHGAKVCFVDVQDELGQHLNESLGDNANTCYFHCDVTMEDDIRRAVDST
Sbjct: 61  GIGEAIVRLFHRHGAKVCFVDVQDELGQHLNESLGDNANTCYFHCDVTMEDDIRRAVDST 120

Query: 121 VSKFGTLDIMVNNAGISGTPCSDIRNVNVLEFERVFDINVKAVFVGMKYAANVMIPRKQG 180
           VSKFGTLDIMVNNAGISGTPCSDIRNVNVLEFERVFDINVKAVFVGMKYAANVMIPRKQG
Sbjct: 121 VSKFGTLDIMVNNAGISGTPCSDIRNVNVLEFERVFDINVKAVFVGMKYAANVMIPRKQG 180

Query: 181 SIISLASVGSVMAGVGPHHYVGSKHAVLGLTRSVAAELGQHGIRVNCVSPYAVPTALAVA 240
           SIISLASVGSVMAGVGPHHYVGSKHAVLGLTRSVAAELGQHGIRVNCVSPYAVPTALAVA
Sbjct: 181 SIISLASVGSVMAGVGPHHYVGSKHAVLGLTRSVAAELGQHGIRVNCVSPYAVPTALAVA 240

Query: 241 HLPEDERTEDVFVGFREFAGKIANLQGVQLEVEDVANAVLFLASDEARYISGDNLLVDGG 300
           HLPEDERTEDVFVGFREFAGKIANLQGVQLEVEDVANAVLFLASDEARYISGDNLLVDGG
Sbjct: 241 HLPEDERTEDVFVGFREFAGKIANLQGVQLEVEDVANAVLFLASDEARYISGDNLLVDGG 300

Query: 301 FTRVNHALRVFR 313
           FTRVNHALRVFR
Sbjct: 301 FTRVNHALRVFR 312

BLAST of Moc04g29480 vs. ExPASy TrEMBL
Match: A0A5D3BMA8 (Xanthoxin dehydrogenase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold446G00650 PE=4 SV=1)

HSP 1 Score: 461.1 bits (1185), Expect = 3.8e-126
Identity = 237/283 (83.75%), Postives = 255/283 (90.11%), Query Frame = 0

Query: 32  MATPSSAESSSLPTQRLLGKVALVTGGASGIGEAIVRLFHRHGAKVCFVDVQDELGQHLN 91
           MATP     SSL +QRLLGKVA VTGGASGIGE IVRLFHRHGAKVCFVDVQDELG  L 
Sbjct: 1   MATP---PISSLVSQRLLGKVAFVTGGASGIGEGIVRLFHRHGAKVCFVDVQDELGYRLL 60

Query: 92  ESLG--DNANTCYFHCDVTMEDDIRRAVDSTVSKFGTLDIMVNNAGISGTPCSDIRNVNV 151
           ESLG   + N  Y HCDVT+EDD+RRAVD TV++FGTLDIMVNNAGISGTP SDIRNV++
Sbjct: 61  ESLGGDKDPNIFYSHCDVTVEDDLRRAVDLTVTRFGTLDIMVNNAGISGTPSSDIRNVDI 120

Query: 152 LEFERVFDINVKAVFVGMKYAANVMIPRKQGSIISLASVGSVMAGVGPHHYVGSKHAVLG 211
            EFE+VFDINVKAVF+GMKYAA+VMIPRKQGSIISL SVGSV+ G+GPHHYVGSKHAV+G
Sbjct: 121 SEFEKVFDINVKAVFMGMKYAASVMIPRKQGSIISLGSVGSVIGGIGPHHYVGSKHAVVG 180

Query: 212 LTRSVAAELGQHGIRVNCVSPYAVPTALAVAHLPEDERTEDVFVGFREFAGKIANLQGVQ 271
           LTRSVAAELGQHGIRVNCVSPYAVPT LAVAHLPEDERTEDVF GFREFA K ANLQGV+
Sbjct: 181 LTRSVAAELGQHGIRVNCVSPYAVPTNLAVAHLPEDERTEDVFTGFREFAKKNANLQGVE 240

Query: 272 LEVEDVANAVLFLASDEARYISGDNLLVDGGFTRVNHALRVFR 313
           L VEDVANAVLFLAS++ARYISGDNLLVDGGFTRVNH+LRVFR
Sbjct: 241 LTVEDVANAVLFLASEDARYISGDNLLVDGGFTRVNHSLRVFR 280

BLAST of Moc04g29480 vs. ExPASy TrEMBL
Match: A0A1S3CPE9 (xanthoxin dehydrogenase OS=Cucumis melo OX=3656 GN=LOC103503321 PE=4 SV=1)

HSP 1 Score: 461.1 bits (1185), Expect = 3.8e-126
Identity = 237/283 (83.75%), Postives = 255/283 (90.11%), Query Frame = 0

Query: 32  MATPSSAESSSLPTQRLLGKVALVTGGASGIGEAIVRLFHRHGAKVCFVDVQDELGQHLN 91
           MATP     SSL +QRLLGKVA VTGGASGIGE IVRLFHRHGAKVCFVDVQDELG  L 
Sbjct: 1   MATP---PISSLVSQRLLGKVAFVTGGASGIGEGIVRLFHRHGAKVCFVDVQDELGYRLL 60

Query: 92  ESLG--DNANTCYFHCDVTMEDDIRRAVDSTVSKFGTLDIMVNNAGISGTPCSDIRNVNV 151
           ESLG   + N  Y HCDVT+EDD+RRAVD TV++FGTLDIMVNNAGISGTP SDIRNV++
Sbjct: 61  ESLGGDKDPNIFYSHCDVTVEDDLRRAVDLTVTRFGTLDIMVNNAGISGTPSSDIRNVDI 120

Query: 152 LEFERVFDINVKAVFVGMKYAANVMIPRKQGSIISLASVGSVMAGVGPHHYVGSKHAVLG 211
            EFE+VFDINVKAVF+GMKYAA+VMIPRKQGSIISL SVGSV+ G+GPHHYVGSKHAV+G
Sbjct: 121 SEFEKVFDINVKAVFMGMKYAASVMIPRKQGSIISLGSVGSVIGGIGPHHYVGSKHAVVG 180

Query: 212 LTRSVAAELGQHGIRVNCVSPYAVPTALAVAHLPEDERTEDVFVGFREFAGKIANLQGVQ 271
           LTRSVAAELGQHGIRVNCVSPYAVPT LAVAHLPEDERTEDVF GFREFA K ANLQGV+
Sbjct: 181 LTRSVAAELGQHGIRVNCVSPYAVPTNLAVAHLPEDERTEDVFTGFREFAKKNANLQGVE 240

Query: 272 LEVEDVANAVLFLASDEARYISGDNLLVDGGFTRVNHALRVFR 313
           L VEDVANAVLFLAS++ARYISGDNLLVDGGFTRVNH+LRVFR
Sbjct: 241 LTVEDVANAVLFLASEDARYISGDNLLVDGGFTRVNHSLRVFR 280

BLAST of Moc04g29480 vs. ExPASy TrEMBL
Match: A0A6J1FL60 (xanthoxin dehydrogenase OS=Cucurbita moschata OX=3662 GN=LOC111445228 PE=4 SV=1)

HSP 1 Score: 456.4 bits (1173), Expect = 9.3e-125
Identity = 232/283 (81.98%), Postives = 257/283 (90.81%), Query Frame = 0

Query: 32  MATPSSAESSSLPTQRLLGKVALVTGGASGIGEAIVRLFHRHGAKVCFVDVQDELGQHLN 91
           MAT  S+ SS +P QRLLGKVALVTGGASGIGEAIVRLFHRHGAKVCFVDVQDELG+ L+
Sbjct: 1   MATAESSSSSLVP-QRLLGKVALVTGGASGIGEAIVRLFHRHGAKVCFVDVQDELGRRLH 60

Query: 92  ESLG--DNANTCYFHCDVTMEDDIRRAVDSTVSKFGTLDIMVNNAGISGTPCSDIRNVNV 151
           E LG  D++NT Y HCDVT+EDD+RRAVD+TVSKFGTLDIMVNNAGISGTP SDIRNV+V
Sbjct: 61  EDLGGNDDSNTFYSHCDVTVEDDVRRAVDTTVSKFGTLDIMVNNAGISGTPTSDIRNVDV 120

Query: 152 LEFERVFDINVKAVFVGMKYAANVMIPRKQGSIISLASVGSVMAGVGPHHYVGSKHAVLG 211
            EFE+VFDINVKAVF+GMK+AA+VMIP KQGSIISLAS+GSV+ G+GPHHY+ SKHAVLG
Sbjct: 121 SEFEKVFDINVKAVFMGMKHAASVMIPNKQGSIISLASIGSVIGGIGPHHYISSKHAVLG 180

Query: 212 LTRSVAAELGQHGIRVNCVSPYAVPTALAVAHLPEDERTEDVFVGFREFAGKIANLQGVQ 271
           LTRSVAAELGQ+GIRVNCVSPYAVPT+LA AHL E+ERTEDV  GFR+F GK ANLQGV 
Sbjct: 181 LTRSVAAELGQYGIRVNCVSPYAVPTSLAFAHLHEEERTEDVITGFRQFIGKNANLQGVD 240

Query: 272 LEVEDVANAVLFLASDEARYISGDNLLVDGGFTRVNHALRVFR 313
           LE EDVANAVLFL S+EARYISGDNLLVDGGF+ VNH+LRVFR
Sbjct: 241 LEAEDVANAVLFLTSNEARYISGDNLLVDGGFSCVNHSLRVFR 282

BLAST of Moc04g29480 vs. ExPASy TrEMBL
Match: A0A6J1JZF0 (xanthoxin dehydrogenase OS=Cucurbita maxima OX=3661 GN=LOC111489670 PE=4 SV=1)

HSP 1 Score: 456.1 bits (1172), Expect = 1.2e-124
Identity = 234/283 (82.69%), Postives = 258/283 (91.17%), Query Frame = 0

Query: 32  MATPSSAESSSLPTQRLLGKVALVTGGASGIGEAIVRLFHRHGAKVCFVDVQDELGQHLN 91
           MAT  S+ SS +P QRLLGKVALVTGGASGIGEAIVRLFHRHGAKVCFVDVQDELG+ L+
Sbjct: 1   MATAESSSSSLVP-QRLLGKVALVTGGASGIGEAIVRLFHRHGAKVCFVDVQDELGRCLH 60

Query: 92  ESLG--DNANTCYFHCDVTMEDDIRRAVDSTVSKFGTLDIMVNNAGISGTPCSDIRNVNV 151
           E LG  D++NT Y HCDVT+EDD+RRAVD TVSKFGTLDIMVNNAGISGTP SDIR+V+V
Sbjct: 61  EDLGGNDDSNTFYSHCDVTVEDDVRRAVDVTVSKFGTLDIMVNNAGISGTPSSDIRSVDV 120

Query: 152 LEFERVFDINVKAVFVGMKYAANVMIPRKQGSIISLASVGSVMAGVGPHHYVGSKHAVLG 211
            EFE+VF+INVKAVF+GMKYAA+VMIP KQGSIISLASVGSV+ G+GPHHY+ SKHAVLG
Sbjct: 121 SEFEKVFNINVKAVFMGMKYAASVMIPNKQGSIISLASVGSVIGGIGPHHYISSKHAVLG 180

Query: 212 LTRSVAAELGQHGIRVNCVSPYAVPTALAVAHLPEDERTEDVFVGFREFAGKIANLQGVQ 271
           LTRSVAAELGQ+GIRVNCVSPYAVPT+LA AHL E+ERTEDV  GFR+F GK ANLQGV 
Sbjct: 181 LTRSVAAELGQYGIRVNCVSPYAVPTSLAFAHLHEEERTEDVITGFRQFTGKNANLQGVD 240

Query: 272 LEVEDVANAVLFLASDEARYISGDNLLVDGGFTRVNHALRVFR 313
           LEVEDVANAVLFLAS+EARYISGDNLLVDGGF+ VNH+LRVFR
Sbjct: 241 LEVEDVANAVLFLASNEARYISGDNLLVDGGFSCVNHSLRVFR 282

BLAST of Moc04g29480 vs. TAIR 10
Match: AT1G52340.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 396.4 bits (1017), Expect = 2.2e-110
Identity = 196/279 (70.25%), Postives = 232/279 (83.15%), Query Frame = 0

Query: 37  SAESSSLPTQRLLGKVALVTGGASGIGEAIVRLFHRHGAKVCFVDVQDELGQHLNESL-- 96
           S+  SSLP+QRLLGKVAL+TGGA+GIGE+IVRLFH+HGAKVC VD+QD+LG  + +SL  
Sbjct: 7   SSSYSSLPSQRLLGKVALITGGATGIGESIVRLFHKHGAKVCIVDLQDDLGGEVCKSLLR 66

Query: 97  GDNANTCYF-HCDVTMEDDIRRAVDSTVSKFGTLDIMVNNAGISGTPCSDIRNVNVLEFE 156
           G++  T +F H DV +EDDI  AVD  V  FGTLDI++NNAG+ G PC DIRN ++ EFE
Sbjct: 67  GESKETAFFIHGDVRVEDDISNAVDFAVKNFGTLDILINNAGLCGAPCPDIRNYSLSEFE 126

Query: 157 RVFDINVKAVFVGMKYAANVMIPRKQGSIISLASVGSVMAGVGPHHYVGSKHAVLGLTRS 216
             FD+NVK  F+ MK+AA VMIP K+GSI+SL SVG V+ GVGPH YVGSKHAVLGLTRS
Sbjct: 127 MTFDVNVKGAFLSMKHAARVMIPEKKGSIVSLCSVGGVVGGVGPHSYVGSKHAVLGLTRS 186

Query: 217 VAAELGQHGIRVNCVSPYAVPTALAVAHLPEDERTEDVFVGFREFAGKIANLQGVQLEVE 276
           VAAELGQHGIRVNCVSPYAV T LA+AHLPE+ERTED FVGFR FA   ANL+GV+L V+
Sbjct: 187 VAAELGQHGIRVNCVSPYAVATKLALAHLPEEERTEDAFVGFRNFAAANANLKGVELTVD 246

Query: 277 DVANAVLFLASDEARYISGDNLLVDGGFTRVNHALRVFR 313
           DVANAVLFLASD++RYISGDNL++DGGFT  NH+ +VFR
Sbjct: 247 DVANAVLFLASDDSRYISGDNLMIDGGFTCTNHSFKVFR 285

BLAST of Moc04g29480 vs. TAIR 10
Match: AT3G51680.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 226.1 bits (575), Expect = 3.9e-59
Identity = 124/282 (43.97%), Postives = 178/282 (63.12%), Query Frame = 0

Query: 40  SSSLPTQRLLGKVALVTGGASGIGEAIVRLFHRHGAKVCFVDVQDELGQHLNESLGDNAN 99
           +++L  +RL GKVA++TGGA GIG+A V LF RHGA V   DV +  G  L +SL  +  
Sbjct: 24  NTTLYPKRLEGKVAIITGGAHGIGKATVMLFARHGATVVIADVDNVAGSSLAKSLSSHKT 83

Query: 100 T---CYFHCDVTMEDDIRRAVDSTVSKFGTLDIMVNNAGISG--TPCSDIRNVNVLEFER 159
           +    +  CDV++E D+   V+ TV+++G LDI+ NNAG+ G       I + +  EF+ 
Sbjct: 84  SPMVAFISCDVSVEADVENLVNVTVARYGRLDILFNNAGVLGDQKKHKSILDFDADEFDH 143

Query: 160 VFDINVKAVFVGMKYAANVMIPRK-QGSIISLASVGSVMAGVGPHHYVGSKHAVLGLTRS 219
           V  +NV+ V +GMK+ A  MI R  +G IIS ASV  VM G+GPH Y  SKHA++GLT++
Sbjct: 144 VMRVNVRGVGLGMKHGARAMIKRGFKGCIISTASVAGVMGGMGPHAYTASKHAIVGLTKN 203

Query: 220 VAAELGQHGIRVNCVSPYAVPTALAV-------AHLPEDERTEDVFVGFREFAGKIANLQ 279
            A ELG++GIRVNC+SP+ V T++ V           ED+  E++     EF   +ANL+
Sbjct: 204 AACELGKYGIRVNCISPFGVATSMLVNAWRKTSGGDVEDDDVEEM----EEFVRSLANLK 263

Query: 280 GVQLEVEDVANAVLFLASDEARYISGDNLLVDGGFTRVNHAL 309
           G  L   D+A A L+LASDE++Y++G NL+VDGG T   + +
Sbjct: 264 GETLRANDIAEAALYLASDESKYVNGHNLVVDGGVTTARNCV 301

BLAST of Moc04g29480 vs. TAIR 10
Match: AT4G03140.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 218.0 bits (554), Expect = 1.1e-56
Identity = 122/274 (44.53%), Postives = 167/274 (60.95%), Query Frame = 0

Query: 36  SSAESSSLPTQRLLGKVALVTGGASGIGEAIVRLFHRHGAKVCFVDVQDELGQHLNESLG 95
           SS+ SS   +++L GKVAL+TGGASGIG+A    F  HGAKV   D+Q ++G+   + LG
Sbjct: 66  SSSSSSHSTSRKLEGKVALITGGASGIGKATAGKFISHGAKVIIADIQPQIGRETEQELG 125

Query: 96  DNANTCYFHCDVTMEDDIRRAVDSTVSKFGTLDIMVNNAGISGTPCSDIRNVNVLEFERV 155
            +    YF CDVT E DI  AVD  VS    LDIM NNAGI       I ++++  F++V
Sbjct: 126 PSC--AYFPCDVTKESDIANAVDFAVSLHTKLDIMYNNAGIPCKTPPSIVDLDLNVFDKV 185

Query: 156 FDINVKAVFVGMKYAANVMIPRKQGSIISLASVGSVMAGVGPHHYVGSKHAVLGLTRSVA 215
            + NV+ V  G+K+AA VMIPR  GSII   SV  +M G+  H Y  SK AV+G+ RS A
Sbjct: 186 INTNVRGVMAGIKHAARVMIPRNSGSIICAGSVTGMMGGLAQHTYSVSKSAVIGIVRSTA 245

Query: 216 AELGQHGIRVNCVSPYAVPTALAVAHLPEDERTEDVFVG-----FREFAGKIANLQGVQL 275
           +EL +H IRVNC+SP+A+ T+  +      +    ++ G       +       L G   
Sbjct: 246 SELCKHRIRVNCISPFAITTSFVM------DEMRQIYPGVDDSRLIQIVQSTGVLNGEVC 305

Query: 276 EVEDVANAVLFLASDEARYISGDNLLVDGGFTRV 305
           E  DVANA ++LASD+++Y++G NL+VDGGFT V
Sbjct: 306 EPTDVANAAVYLASDDSKYVNGHNLVVDGGFTTV 331

BLAST of Moc04g29480 vs. TAIR 10
Match: AT2G47130.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 214.5 bits (545), Expect = 1.2e-55
Identity = 117/259 (45.17%), Postives = 166/259 (64.09%), Query Frame = 0

Query: 47  RLLGKVALVTGGASGIGEAIVRLFHRHGAKVCFVDVQDELGQHLNESLGDNANTCYFHCD 106
           RL GK+A++TGGASGIG   VRLF  HGAKV  VD Q+ELGQ++  S+G +    ++ CD
Sbjct: 5   RLDGKIAIITGGASGIGAEAVRLFTDHGAKVVIVDFQEELGQNVAVSVGKD-KASFYRCD 64

Query: 107 VTMEDDIRRAVDSTVSKFGTLDIMVNNAGISGTPCSDIRNVNVLEFERVFDINVKAVFVG 166
           VT E ++  AV  TV K+G LD++ +NAG+   P S + ++N+ +F+R   +NV+     
Sbjct: 65  VTNEKEVENAVKFTVEKYGKLDVLFSNAGVMEQPGSFL-DLNLEQFDRTMAVNVRGAAAF 124

Query: 167 MKYAANVMIPR-KQGSIISLASVGSVMAGVGPHHYVGSKHAVLGLTRSVAAELGQHGIRV 226
           +K+AA  M+ +  +GSI+   SV S + G GPH Y  SKHA+LGL +S    LG++GIRV
Sbjct: 125 IKHAARAMVEKGTRGSIVCTTSVASEIGGPGPHAYTASKHALLGLVKSACGGLGKYGIRV 184

Query: 227 NCVSPYAVPTALAVAHLPEDERTEDVFVGFREFAGKIANLQGVQLEVEDVANAVLFLASD 286
           N V+PYAV TA+       + R E+      E++     L+GV L+   VA A LFLASD
Sbjct: 185 NGVAPYAVATAI-------NSRDEETVRMVEEYSAATGILKGVVLKARHVAEAALFLASD 244

Query: 287 EARYISGDNLLVDGGFTRV 305
           ++ Y+SG NL VDGG++ V
Sbjct: 245 DSAYVSGQNLAVDGGYSVV 254

BLAST of Moc04g29480 vs. TAIR 10
Match: AT3G29250.2 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 211.8 bits (538), Expect = 7.6e-55
Identity = 118/260 (45.38%), Postives = 165/260 (63.46%), Query Frame = 0

Query: 47  RLLGKVALVTGGASGIGEAIVRLFHRHGAKVCFVDVQDELGQHLNESLG-DNANTCYFHC 106
           RL GK+A++TGGASGIG   VRLF  HGAKV  VD+Q+ELGQ+L  S+G D A+  ++ C
Sbjct: 5   RLDGKIAIITGGASGIGAEAVRLFTDHGAKVVIVDIQEELGQNLAVSIGLDKAS--FYRC 64

Query: 107 DVTMEDDIRRAVDSTVSKFGTLDIMVNNAGISGTPCSDIRNVNVLEFERVFDINVKAVFV 166
           +VT E D+  AV  TV K G LD++ +NAG+       + ++++  F+R   +NV+    
Sbjct: 65  NVTDETDVENAVKFTVEKHGKLDVLFSNAGVL-EAFGSVLDLDLEAFDRTMAVNVRGAAA 124

Query: 167 GMKYAANVMIPR-KQGSIISLASVGSVMAGVGPHHYVGSKHAVLGLTRSVAAELGQHGIR 226
            +K+AA  M+    +GSI+   S+ + + G GPH Y  SKHA+LGL RS  A LGQ+GIR
Sbjct: 125 FIKHAARSMVASGTRGSIVCTTSIAAEIGGPGPHSYTASKHALLGLIRSACAGLGQYGIR 184

Query: 227 VNCVSPYAVPTALAVAHLPEDERTEDVFVGFREFAGKIANLQGVQLEVEDVANAVLFLAS 286
           VN V+PY V T +  A+       E+      E+   + NL+GV L+   +A A LFLAS
Sbjct: 185 VNGVAPYGVATGMTSAY------NEEAVKMLEEYGEALGNLKGVVLKARHIAEAALFLAS 244

Query: 287 DEARYISGDNLLVDGGFTRV 305
           D++ YISG NL+VDGGF+ V
Sbjct: 245 DDSVYISGQNLVVDGGFSVV 255

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022157898.14.2e-172100.00xanthoxin dehydrogenase [Momordica charantia][more]
XP_038906598.12.9e-12885.66xanthoxin dehydrogenase [Benincasa hispida][more]
XP_023549777.17.8e-12683.04xanthoxin dehydrogenase [Cucurbita pepo subsp. pepo][more]
XP_008465699.17.8e-12683.75PREDICTED: xanthoxin dehydrogenase [Cucumis melo] >TYJ99851.1 xanthoxin dehydrog... [more]
KAG6579113.16.6e-12582.69Xanthoxin dehydrogenase, partial [Cucurbita argyrosperma subsp. sororia] >KAG701... [more]
Match NameE-valueIdentityDescription
Q9C8263.1e-10970.25Xanthoxin dehydrogenase OS=Arabidopsis thaliana OX=3702 GN=ABA2 PE=1 SV=1[more]
F1SWA05.4e-9865.28Zerumbone synthase OS=Zingiber zerumbet OX=311405 GN=ZSD1 PE=1 SV=1[more]
Q94KL73.2e-7455.43Secoisolariciresinol dehydrogenase (Fragment) OS=Forsythia intermedia OX=55183 P... [more]
Q7FAE15.5e-6648.94Momilactone A synthase OS=Oryza sativa subsp. japonica OX=39947 GN=Os04g0179200 ... [more]
A3F5F02.0e-6347.79Secoisolariciresinol dehydrogenase OS=Sinopodophyllum hexandrum OX=93608 GN=SDH ... [more]
Match NameE-valueIdentityDescription
A0A6J1DUL72.0e-172100.00xanthoxin dehydrogenase OS=Momordica charantia OX=3673 GN=LOC111024504 PE=4 SV=1[more]
A0A5D3BMA83.8e-12683.75Xanthoxin dehydrogenase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold... [more]
A0A1S3CPE93.8e-12683.75xanthoxin dehydrogenase OS=Cucumis melo OX=3656 GN=LOC103503321 PE=4 SV=1[more]
A0A6J1FL609.3e-12581.98xanthoxin dehydrogenase OS=Cucurbita moschata OX=3662 GN=LOC111445228 PE=4 SV=1[more]
A0A6J1JZF01.2e-12482.69xanthoxin dehydrogenase OS=Cucurbita maxima OX=3661 GN=LOC111489670 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
AT1G52340.12.2e-11070.25NAD(P)-binding Rossmann-fold superfamily protein [more]
AT3G51680.13.9e-5943.97NAD(P)-binding Rossmann-fold superfamily protein [more]
AT4G03140.11.1e-5644.53NAD(P)-binding Rossmann-fold superfamily protein [more]
AT2G47130.11.2e-5545.17NAD(P)-binding Rossmann-fold superfamily protein [more]
AT3G29250.27.6e-5545.38NAD(P)-binding Rossmann-fold superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (OHB3-1) v2
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002347Short-chain dehydrogenase/reductase SDRPRINTSPR00080SDRFAMILYcoord: 200..219
score: 39.43
coord: 180..188
score: 45.55
coord: 125..136
score: 57.32
IPR002347Short-chain dehydrogenase/reductase SDRPRINTSPR00081GDHRDHcoord: 52..69
score: 55.84
coord: 265..285
score: 35.61
coord: 125..136
score: 61.97
coord: 174..190
score: 31.14
coord: 200..219
score: 41.8
coord: 221..238
score: 40.89
NoneNo IPR availableGENE3D3.40.50.720coord: 37..311
e-value: 2.2E-77
score: 261.8
NoneNo IPR availablePFAMPF13561adh_short_C2coord: 57..302
e-value: 9.9E-65
score: 218.4
NoneNo IPR availablePIRSRPIRSR629511-2PIRSR629511-2coord: 45..301
e-value: 2.9E-31
score: 106.4
NoneNo IPR availablePIRSRPIRSR000095-1PIRSR000095-1coord: 54..249
e-value: 1.6E-17
score: 61.5
NoneNo IPR availablePANTHERPTHR42820:SF1XANTHOXIN DEHYDROGENASEcoord: 41..312
NoneNo IPR availablePANTHERPTHR42820SHORT-CHAIN DEHYDROGENASE REDUCTASEcoord: 41..312
NoneNo IPR availableCDDcd05326secoisolariciresinol-DH_like_SDR_ccoord: 47..302
e-value: 3.05044E-124
score: 353.684
IPR036291NAD(P)-binding domain superfamilySUPERFAMILY51735NAD(P)-binding Rossmann-fold domainscoord: 50..303

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Moc04g29480.1Moc04g29480.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0009688 abscisic acid biosynthetic process
cellular_component GO:0005829 cytosol
molecular_function GO:0010301 xanthoxin dehydrogenase activity
molecular_function GO:0016491 oxidoreductase activity