Moc04g28790 (gene) Bitter gourd (OHB3-1) v2

Overview
NameMoc04g28790
Typegene
OrganismMomordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
DescriptionPectinesterase
Locationchr4: 21385626 .. 21387519 (+)
RNA-Seq ExpressionMoc04g28790
SyntenyMoc04g28790
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGTGGGAAAAGCGGCAGTGTCGGTTCTATCCCTTATTTTAGTGGTGGGAGTTGCTTTGGCAGTGGTGGCAGTTGTTAACATGAACAAATCCGGCAGTTTGGACACTGAGAATATGTCCCCCAAAATGAAAGCTATTGCTTCCATATGTTCTCAGACTGATTACCAAAAAGAATGTCAAGATACTCTGGGGGTCCTGGCTAAGAATTCTACCTCGGACGACCCGAAGGAGTACATTAAAACAGCTATTATGGCGACTGTTGATGAGATTAAGAAAGGGTTCAATTTGACGGACTCCCTTATGGTGGAGGCTGGTAACAGTGCCTTCATGAAGATGTCGGTTGAAGATTGTAAGGATTTGCTCCGCTTTGCGGTTAGAGAGCTCCAGGCTTCTTACTCCAGCGTTGGGGCCAACGATGAGCGCTCCCAAGAGGAGCGAGTCTCGGATATCAATACCTGGCTCAGCGCCGTCATTTCTTATCAGGAATCTTGCCTCGACGCCCTTGGAGAATCCAACCCCAAGCTCAAAGAAACCATGGGAGCTGGTAGTCTCGACTTTGCAACCAAGCTTACCAGCAACGCTCTCGCTATTTTCTCTGAGGTCTCGAAGATTCTCGGTAATTATGGCCTGCAATTAAAGGTACTCTACCGATACACATATACCACCATAGTCTGTACTAAATCACCACTAAACTTAAAAACTCTAATAATTTAAGTTTTATTAACAGGCCCCTCAGCCAAATGGACGCCGTTTGCTCGGACACACAGAAGTGGGGAGCGACGGTTTCCCAACATGGTTTTCCAAGGCGGACAGGAAGCTGCTGGCGGCGGCGAGGAGAGGAGGAGGAGGAGTGGTGGGAGTGAAACCGAACGTGGTGGTGGCAAAGGACGGGAGTGGGAATTACAAGACCATTGGTGCAGCATTGGCTGCAGCTCCAAAGAATTCCAAGACACGATATGTGATCTACGTGAAAGCAGGGATCTATGATGAGTATGTCACTGTAACTTCAGACTTGACCTACATTTTCATGTACGGAGATGGCCCGAGAAAAACCATCGTCACTGGCCGCAAGTCCGCCCGCGACGGCATCAACACCATGAACACCGCCACCTTTGGTAAGATATTTTTCTCAAAATCACTCATTCCATGCCATCATTGCCAAACACACCTTTACTTATTAATTACTACTATAATTGCAGCTGCCGTTGGATTTCACTTCCTCTGCAAAGCAATGGGATTCCAAAACACAGCCGGTCCGGAAGGGCACCAAGCGGTGGCCCTCCGCGTCCAGTCGGACAGATCGGCGTTCTATGAATGCCGAATAGACGGCTACCAAGACAGTCTCTACGCCCAAACTAACCGTCAATTCTACCGCAACTGCGTCATCTCCGGCACTGTCGATTTCATCTTCGGCGAGTCGAGCACCATCATCCAGAACAGCCTCATCATCGTCCGGCGGCCGATGCAGGGCCAGCAGAACACGGTGACCGCCGACGGGCGCAAGGAAAACAGGGAGCTCAGCGGGCTGGTGATCCACAACTGCCGAATCGTGCCAGAGCAGAAGCTGTTCGCGGACCGGTTCAAGATCCCCACATTCTTGGGACGGCCGTGGAAGAAGTACGCACTGACAGTAATCATGGAGAGCACATTGGGGGATTTGATCCAGCCCGCCGGGTACATGCCGTGGGAAGAAGATTCCCGAGACACGTGTCAGTACCTGGAGTACGCAAACAGGGGACCGGGAGCCAACACTGCGAGGAGAGTGAAGTGGAAGGGAGTTAGGGTTATTGGAAGGAACGAGGCTCTTAAGTTTACGGTTGGATCACCATTTCTCATCGGAAATGTCTGGCTTCCGGAAACCCGCGGAAAATTCTTACCTGGATTGAAGAACTAG

mRNA sequence

ATGGTGGGAAAAGCGGCAGTGTCGGTTCTATCCCTTATTTTAGTGGTGGGAGTTGCTTTGGCAGTGGTGGCAGTTGTTAACATGAACAAATCCGGCAGTTTGGACACTGAGAATATGTCCCCCAAAATGAAAGCTATTGCTTCCATATGTTCTCAGACTGATTACCAAAAAGAATGTCAAGATACTCTGGGGGTCCTGGCTAAGAATTCTACCTCGGACGACCCGAAGGAGTACATTAAAACAGCTATTATGGCGACTGTTGATGAGATTAAGAAAGGGTTCAATTTGACGGACTCCCTTATGGTGGAGGCTGGTAACAGTGCCTTCATGAAGATGTCGGTTGAAGATTGTAAGGATTTGCTCCGCTTTGCGGTTAGAGAGCTCCAGGCTTCTTACTCCAGCGTTGGGGCCAACGATGAGCGCTCCCAAGAGGAGCGAGTCTCGGATATCAATACCTGGCTCAGCGCCGTCATTTCTTATCAGGAATCTTGCCTCGACGCCCTTGGAGAATCCAACCCCAAGCTCAAAGAAACCATGGGAGCTGGTAGTCTCGACTTTGCAACCAAGCTTACCAGCAACGCTCTCGCTATTTTCTCTGAGGTCTCGAAGATTCTCGGTAATTATGGCCTGCAATTAAAGGCCCCTCAGCCAAATGGACGCCGTTTGCTCGGACACACAGAAGTGGGGAGCGACGGTTTCCCAACATGGTTTTCCAAGGCGGACAGGAAGCTGCTGGCGGCGGCGAGGAGAGGAGGAGGAGGAGTGGTGGGAGTGAAACCGAACGTGGTGGTGGCAAAGGACGGGAGTGGGAATTACAAGACCATTGGTGCAGCATTGGCTGCAGCTCCAAAGAATTCCAAGACACGATATGTGATCTACGTGAAAGCAGGGATCTATGATGAGTATGTCACTGTAACTTCAGACTTGACCTACATTTTCATGTACGGAGATGGCCCGAGAAAAACCATCGTCACTGGCCGCAAGTCCGCCCGCGACGGCATCAACACCATGAACACCGCCACCTTTGCTGCCGTTGGATTTCACTTCCTCTGCAAAGCAATGGGATTCCAAAACACAGCCGGTCCGGAAGGGCACCAAGCGGTGGCCCTCCGCGTCCAGTCGGACAGATCGGCGTTCTATGAATGCCGAATAGACGGCTACCAAGACAGTCTCTACGCCCAAACTAACCGTCAATTCTACCGCAACTGCGTCATCTCCGGCACTGTCGATTTCATCTTCGGCGAGTCGAGCACCATCATCCAGAACAGCCTCATCATCGTCCGGCGGCCGATGCAGGGCCAGCAGAACACGGTGACCGCCGACGGGCGCAAGGAAAACAGGGAGCTCAGCGGGCTGGTGATCCACAACTGCCGAATCGTGCCAGAGCAGAAGCTGTTCGCGGACCGGTTCAAGATCCCCACATTCTTGGGACGGCCGTGGAAGAAGTACGCACTGACAGTAATCATGGAGAGCACATTGGGGGATTTGATCCAGCCCGCCGGGTACATGCCGTGGGAAGAAGATTCCCGAGACACGTGTCAGTACCTGGAGTACGCAAACAGGGGACCGGGAGCCAACACTGCGAGGAGAGTGAAGTGGAAGGGAGTTAGGGTTATTGGAAGGAACGAGGCTCTTAAGTTTACGGTTGGATCACCATTTCTCATCGGAAATGTCTGGCTTCCGGAAACCCGCGGAAAATTCTTACCTGGATTGAAGAACTAG

Coding sequence (CDS)

ATGGTGGGAAAAGCGGCAGTGTCGGTTCTATCCCTTATTTTAGTGGTGGGAGTTGCTTTGGCAGTGGTGGCAGTTGTTAACATGAACAAATCCGGCAGTTTGGACACTGAGAATATGTCCCCCAAAATGAAAGCTATTGCTTCCATATGTTCTCAGACTGATTACCAAAAAGAATGTCAAGATACTCTGGGGGTCCTGGCTAAGAATTCTACCTCGGACGACCCGAAGGAGTACATTAAAACAGCTATTATGGCGACTGTTGATGAGATTAAGAAAGGGTTCAATTTGACGGACTCCCTTATGGTGGAGGCTGGTAACAGTGCCTTCATGAAGATGTCGGTTGAAGATTGTAAGGATTTGCTCCGCTTTGCGGTTAGAGAGCTCCAGGCTTCTTACTCCAGCGTTGGGGCCAACGATGAGCGCTCCCAAGAGGAGCGAGTCTCGGATATCAATACCTGGCTCAGCGCCGTCATTTCTTATCAGGAATCTTGCCTCGACGCCCTTGGAGAATCCAACCCCAAGCTCAAAGAAACCATGGGAGCTGGTAGTCTCGACTTTGCAACCAAGCTTACCAGCAACGCTCTCGCTATTTTCTCTGAGGTCTCGAAGATTCTCGGTAATTATGGCCTGCAATTAAAGGCCCCTCAGCCAAATGGACGCCGTTTGCTCGGACACACAGAAGTGGGGAGCGACGGTTTCCCAACATGGTTTTCCAAGGCGGACAGGAAGCTGCTGGCGGCGGCGAGGAGAGGAGGAGGAGGAGTGGTGGGAGTGAAACCGAACGTGGTGGTGGCAAAGGACGGGAGTGGGAATTACAAGACCATTGGTGCAGCATTGGCTGCAGCTCCAAAGAATTCCAAGACACGATATGTGATCTACGTGAAAGCAGGGATCTATGATGAGTATGTCACTGTAACTTCAGACTTGACCTACATTTTCATGTACGGAGATGGCCCGAGAAAAACCATCGTCACTGGCCGCAAGTCCGCCCGCGACGGCATCAACACCATGAACACCGCCACCTTTGCTGCCGTTGGATTTCACTTCCTCTGCAAAGCAATGGGATTCCAAAACACAGCCGGTCCGGAAGGGCACCAAGCGGTGGCCCTCCGCGTCCAGTCGGACAGATCGGCGTTCTATGAATGCCGAATAGACGGCTACCAAGACAGTCTCTACGCCCAAACTAACCGTCAATTCTACCGCAACTGCGTCATCTCCGGCACTGTCGATTTCATCTTCGGCGAGTCGAGCACCATCATCCAGAACAGCCTCATCATCGTCCGGCGGCCGATGCAGGGCCAGCAGAACACGGTGACCGCCGACGGGCGCAAGGAAAACAGGGAGCTCAGCGGGCTGGTGATCCACAACTGCCGAATCGTGCCAGAGCAGAAGCTGTTCGCGGACCGGTTCAAGATCCCCACATTCTTGGGACGGCCGTGGAAGAAGTACGCACTGACAGTAATCATGGAGAGCACATTGGGGGATTTGATCCAGCCCGCCGGGTACATGCCGTGGGAAGAAGATTCCCGAGACACGTGTCAGTACCTGGAGTACGCAAACAGGGGACCGGGAGCCAACACTGCGAGGAGAGTGAAGTGGAAGGGAGTTAGGGTTATTGGAAGGAACGAGGCTCTTAAGTTTACGGTTGGATCACCATTTCTCATCGGAAATGTCTGGCTTCCGGAAACCCGCGGAAAATTCTTACCTGGATTGAAGAACTAG

Protein sequence

MVGKAAVSVLSLILVVGVALAVVAVVNMNKSGSLDTENMSPKMKAIASICSQTDYQKECQDTLGVLAKNSTSDDPKEYIKTAIMATVDEIKKGFNLTDSLMVEAGNSAFMKMSVEDCKDLLRFAVRELQASYSSVGANDERSQEERVSDINTWLSAVISYQESCLDALGESNPKLKETMGAGSLDFATKLTSNALAIFSEVSKILGNYGLQLKAPQPNGRRLLGHTEVGSDGFPTWFSKADRKLLAAARRGGGGVVGVKPNVVVAKDGSGNYKTIGAALAAAPKNSKTRYVIYVKAGIYDEYVTVTSDLTYIFMYGDGPRKTIVTGRKSARDGINTMNTATFAAVGFHFLCKAMGFQNTAGPEGHQAVALRVQSDRSAFYECRIDGYQDSLYAQTNRQFYRNCVISGTVDFIFGESSTIIQNSLIIVRRPMQGQQNTVTADGRKENRELSGLVIHNCRIVPEQKLFADRFKIPTFLGRPWKKYALTVIMESTLGDLIQPAGYMPWEEDSRDTCQYLEYANRGPGANTARRVKWKGVRVIGRNEALKFTVGSPFLIGNVWLPETRGKFLPGLKN
Homology
BLAST of Moc04g28790 vs. NCBI nr
Match: XP_022146322.1 (pectinesterase-like [Momordica charantia])

HSP 1 Score: 1133.6 bits (2931), Expect = 0.0e+00
Identity = 573/573 (100.00%), Postives = 573/573 (100.00%), Query Frame = 0

Query: 1   MVGKAAVSVLSLILVVGVALAVVAVVNMNKSGSLDTENMSPKMKAIASICSQTDYQKECQ 60
           MVGKAAVSVLSLILVVGVALAVVAVVNMNKSGSLDTENMSPKMKAIASICSQTDYQKECQ
Sbjct: 1   MVGKAAVSVLSLILVVGVALAVVAVVNMNKSGSLDTENMSPKMKAIASICSQTDYQKECQ 60

Query: 61  DTLGVLAKNSTSDDPKEYIKTAIMATVDEIKKGFNLTDSLMVEAGNSAFMKMSVEDCKDL 120
           DTLGVLAKNSTSDDPKEYIKTAIMATVDEIKKGFNLTDSLMVEAGNSAFMKMSVEDCKDL
Sbjct: 61  DTLGVLAKNSTSDDPKEYIKTAIMATVDEIKKGFNLTDSLMVEAGNSAFMKMSVEDCKDL 120

Query: 121 LRFAVRELQASYSSVGANDERSQEERVSDINTWLSAVISYQESCLDALGESNPKLKETMG 180
           LRFAVRELQASYSSVGANDERSQEERVSDINTWLSAVISYQESCLDALGESNPKLKETMG
Sbjct: 121 LRFAVRELQASYSSVGANDERSQEERVSDINTWLSAVISYQESCLDALGESNPKLKETMG 180

Query: 181 AGSLDFATKLTSNALAIFSEVSKILGNYGLQLKAPQPNGRRLLGHTEVGSDGFPTWFSKA 240
           AGSLDFATKLTSNALAIFSEVSKILGNYGLQLKAPQPNGRRLLGHTEVGSDGFPTWFSKA
Sbjct: 181 AGSLDFATKLTSNALAIFSEVSKILGNYGLQLKAPQPNGRRLLGHTEVGSDGFPTWFSKA 240

Query: 241 DRKLLAAARRGGGGVVGVKPNVVVAKDGSGNYKTIGAALAAAPKNSKTRYVIYVKAGIYD 300
           DRKLLAAARRGGGGVVGVKPNVVVAKDGSGNYKTIGAALAAAPKNSKTRYVIYVKAGIYD
Sbjct: 241 DRKLLAAARRGGGGVVGVKPNVVVAKDGSGNYKTIGAALAAAPKNSKTRYVIYVKAGIYD 300

Query: 301 EYVTVTSDLTYIFMYGDGPRKTIVTGRKSARDGINTMNTATFAAVGFHFLCKAMGFQNTA 360
           EYVTVTSDLTYIFMYGDGPRKTIVTGRKSARDGINTMNTATFAAVGFHFLCKAMGFQNTA
Sbjct: 301 EYVTVTSDLTYIFMYGDGPRKTIVTGRKSARDGINTMNTATFAAVGFHFLCKAMGFQNTA 360

Query: 361 GPEGHQAVALRVQSDRSAFYECRIDGYQDSLYAQTNRQFYRNCVISGTVDFIFGESSTII 420
           GPEGHQAVALRVQSDRSAFYECRIDGYQDSLYAQTNRQFYRNCVISGTVDFIFGESSTII
Sbjct: 361 GPEGHQAVALRVQSDRSAFYECRIDGYQDSLYAQTNRQFYRNCVISGTVDFIFGESSTII 420

Query: 421 QNSLIIVRRPMQGQQNTVTADGRKENRELSGLVIHNCRIVPEQKLFADRFKIPTFLGRPW 480
           QNSLIIVRRPMQGQQNTVTADGRKENRELSGLVIHNCRIVPEQKLFADRFKIPTFLGRPW
Sbjct: 421 QNSLIIVRRPMQGQQNTVTADGRKENRELSGLVIHNCRIVPEQKLFADRFKIPTFLGRPW 480

Query: 481 KKYALTVIMESTLGDLIQPAGYMPWEEDSRDTCQYLEYANRGPGANTARRVKWKGVRVIG 540
           KKYALTVIMESTLGDLIQPAGYMPWEEDSRDTCQYLEYANRGPGANTARRVKWKGVRVIG
Sbjct: 481 KKYALTVIMESTLGDLIQPAGYMPWEEDSRDTCQYLEYANRGPGANTARRVKWKGVRVIG 540

Query: 541 RNEALKFTVGSPFLIGNVWLPETRGKFLPGLKN 574
           RNEALKFTVGSPFLIGNVWLPETRGKFLPGLKN
Sbjct: 541 RNEALKFTVGSPFLIGNVWLPETRGKFLPGLKN 573

BLAST of Moc04g28790 vs. NCBI nr
Match: XP_022146333.1 (pectinesterase-like [Momordica charantia])

HSP 1 Score: 917.9 bits (2371), Expect = 4.3e-263
Identity = 468/573 (81.68%), Postives = 512/573 (89.35%), Query Frame = 0

Query: 2   VGKAAVSVLSLILVVGVALAVVAVVNMNKSGSLDTENMSPKMKAIASICSQTDYQKECQD 61
           +G A VSVLSLILVVGVALAVVAVVN N  GS D EN+SPKMKA+A+ICS TDYQ+ECQ+
Sbjct: 1   MGTAVVSVLSLILVVGVALAVVAVVNRN-HGSFDGENLSPKMKAVATICSHTDYQEECQN 60

Query: 62  TLGVLAKNSTSDDPKEYIKTAIMATVDEIKKGFNLTDSLMVEAGNSAFMKMSVEDCKDLL 121
           TLG +A NS+SDDPKEYIK+AIMATV+E KKGF LTDSLMVEAGN++FMKMSVEDCKDLL
Sbjct: 61  TLGSIAHNSSSDDPKEYIKSAIMATVEEFKKGFKLTDSLMVEAGNNSFMKMSVEDCKDLL 120

Query: 122 RFAVRELQASYSSVGANDERSQEERVSDINTWLSAVISYQESCLDALGESNPKLKETMGA 181
           +FAV ELQASYSSVG +DERSQEERV+DINTWLSAVISYQ+SCLD LGE+NPKLKETM  
Sbjct: 121 QFAVEELQASYSSVGDSDERSQEERVADINTWLSAVISYQQSCLDGLGEANPKLKETM-E 180

Query: 182 GSLDFATKLTSNALAIFSEVSKILGNYGLQLKAPQPNGRRLLGHTEVGSDGFPTWFSKAD 241
           G +D A KLTSNALAI ++VS++LG+YGLQLK  QP GRRLLG+TEVGSDGFPTW S AD
Sbjct: 181 GGIDIARKLTSNALAIVADVSQVLGDYGLQLKV-QPTGRRLLGNTEVGSDGFPTWLSGAD 240

Query: 242 RKLLAAARRGGGGVVGVKPNVVVAKDGSGNYKTIGAALAAAPKNSKTRYVIYVKAGIYDE 301
           R+LLAAA+ GGGG   V PN VVAKDGSG YKTI AALAAAPKNSKTR+VIYVKAGIY+E
Sbjct: 241 RRLLAAAKGGGGG--KVTPNAVVAKDGSGQYKTIAAALAAAPKNSKTRHVIYVKAGIYNE 300

Query: 302 YVTVTSDLTYIFMYGDGPRKTIVTGRKSARDGINTMNTATFAAVGFHFLCKAMGFQNTAG 361
           ++ VT D T IFMYGDGPRKTIVTGRKS RDGI TMNTATF+AVG  FLCKAMGFQNTAG
Sbjct: 301 FIIVTKDYTNIFMYGDGPRKTIVTGRKSFRDGITTMNTATFSAVGNGFLCKAMGFQNTAG 360

Query: 362 PEGHQAVALRVQSDRSAFYECRIDGYQDSLYAQTNRQFYRNCVISGTVDFIFGESSTIIQ 421
           PEGHQAVALRVQSDRSAF+ CR+DGYQD+LY   NRQFYRNCVISGTVDFIFG+S+TIIQ
Sbjct: 361 PEGHQAVALRVQSDRSAFFNCRMDGYQDTLYVHANRQFYRNCVISGTVDFIFGDSNTIIQ 420

Query: 422 NSLIIVRRPMQGQQNTVTADGRKENRELSGLVIHNCRIVPEQKLFADRFKIPTFLGRPWK 481
           NSLIIVRRPM  QQNTVTA+GRKEN+E SGLVIHNCRIVPEQKLFADRFKIPTFLGRPWK
Sbjct: 421 NSLIIVRRPMDNQQNTVTANGRKENKEFSGLVIHNCRIVPEQKLFADRFKIPTFLGRPWK 480

Query: 482 KYALTVIMESTLGDLIQPAGYMPWEED-SRDTCQYLEYANRGPGANTARRVKWKGVRVIG 541
           +YALTVIME+TLGD IQPAGYMPW+   + DTCQYLEYANRGPGANTARRVKWKGVRV+G
Sbjct: 481 EYALTVIMETTLGDFIQPAGYMPWQGTFALDTCQYLEYANRGPGANTARRVKWKGVRVMG 540

Query: 542 RNEALKFTVGSPFLIGNVWLPETRGKFLPGLKN 574
           RNEALKFT GSPFL+G  WLP T G FL GLKN
Sbjct: 541 RNEALKFTAGSPFLLGKEWLPATGGNFLLGLKN 568

BLAST of Moc04g28790 vs. NCBI nr
Match: KAA0050274.1 (pectinesterase-like [Cucumis melo var. makuwa] >TYJ98133.1 pectinesterase-like [Cucumis melo var. makuwa])

HSP 1 Score: 800.0 bits (2065), Expect = 1.3e-227
Identity = 408/574 (71.08%), Postives = 477/574 (83.10%), Query Frame = 0

Query: 1   MVGKAAVSVLSLILVVGVALAVVAVVNMNKSGSLDTENMSPKMKAIASICSQTDYQKECQ 60
           MVGK  VS LSLILVVG+ALAVVA +N     +   E +SPKMKAI++ICS TDYQ+EC+
Sbjct: 1   MVGKVIVSALSLILVVGIALAVVATINKRNGSNGGKEEVSPKMKAISAICSTTDYQEECK 60

Query: 61  DTLGVLAKNSTSDDPKEYIKTAIMATVDEIKKGFNLTDSLMVEAGNSAFMKMSVEDCKDL 120
           +TL  +A N++S+DPK+Y K AI+AT+ EI KG+NL+DSL+VEAGN+A +KMSVEDCKDL
Sbjct: 61  NTLNHVAHNTSSNDPKDYAKAAILATIKEITKGYNLSDSLIVEAGNNASIKMSVEDCKDL 120

Query: 121 LRFAVRELQASYSSVGANDERSQEERVSDINTWLSAVISYQESCLDALGESNPKLKETMG 180
           L+FA+ ELQASYS+VG +D  +  +R++DI  WLSAV+SYQ+SCLD LGE +P+LK+ M 
Sbjct: 121 LQFAIDELQASYSTVGESDLHTDADRIADIKNWLSAVVSYQQSCLDGLGEFDPQLKQRMQ 180

Query: 181 AGSLDFATKLTSNALAIFSEVSKILGNYGLQLKAPQPNGRRLLGHTEVGSDGFPTWFSKA 240
            G LD A KLTSNALAI + VS IL NYGLQLK  QP+GRRLLG TEV SDGFPTW + A
Sbjct: 181 DG-LDVAGKLTSNALAIVTAVSNILDNYGLQLKV-QPSGRRLLGTTEVDSDGFPTWLTGA 240

Query: 241 DRKLLAAARRGGGGVVGVKPNVVVAKDGSGNYKTIGAALAAAPKNSKTRYVIYVKAGIYD 300
           DRKLL A+++GG   V   PN VVAKDGSG YKTI AALAA PK  + RYVIYVKAGIYD
Sbjct: 241 DRKLL-ASKQGG---VRPTPNAVVAKDGSGQYKTIAAALAAYPKALRGRYVIYVKAGIYD 300

Query: 301 EYVTVTSDLTYIFMYGDGPRKTIVTGRKSARDGINTMNTATFAAVGFHFLCKAMGFQNTA 360
           EY+TVT D+  +FMYGDGPRKTIVTGRKS RDG  T NTA+FAA+G  FLCK+MGF NTA
Sbjct: 301 EYITVTKDMKNVFMYGDGPRKTIVTGRKSNRDGFTTQNTASFAAIGEGFLCKSMGFTNTA 360

Query: 361 GPEGHQAVALRVQSDRSAFYECRIDGYQDSLYAQTNRQFYRNCVISGTVDFIFGESSTII 420
           GP+GHQAVALRVQSDRSAF+ CR+DG+QD+LY QT+RQFYRNCVISGTVDFIFG+SSTII
Sbjct: 361 GPQGHQAVALRVQSDRSAFFNCRMDGHQDTLYVQTHRQFYRNCVISGTVDFIFGDSSTII 420

Query: 421 QNSLIIVRRPMQGQQNTVTADGRKENRELSGLVIHNCRIVPEQKLFADRFKIPTFLGRPW 480
           QNSLIIVRRPM  QQNTVTA GR E +E++GLVIHNCRIVPEQKLFA+RFKIPTFLGRPW
Sbjct: 421 QNSLIIVRRPMDNQQNTVTAQGRAEQKEITGLVIHNCRIVPEQKLFAERFKIPTFLGRPW 480

Query: 481 KKYALTVIMESTLGDLIQPAGYMPWEED-SRDTCQYLEYANRGPGANTARRVKWKGVRVI 540
           K+YA TVIMESTLGD IQPAGYMPW  + + +TC YLEY NRGPGANT RRV+WKGV+VI
Sbjct: 481 KQYARTVIMESTLGDFIQPAGYMPWSGNFALETCLYLEYGNRGPGANTNRRVRWKGVKVI 540

Query: 541 GRNEALKFTVGSPFLIGNVWLPETRGKFLPGLKN 574
           GRNEAL++T G+ FL+G  WLP T G +  GLKN
Sbjct: 541 GRNEALQYTAGA-FLLGRTWLPRTGGIYYLGLKN 567

BLAST of Moc04g28790 vs. NCBI nr
Match: XP_008466403.1 (PREDICTED: pectinesterase-like [Cucumis melo])

HSP 1 Score: 799.3 bits (2063), Expect = 2.2e-227
Identity = 407/574 (70.91%), Postives = 477/574 (83.10%), Query Frame = 0

Query: 1   MVGKAAVSVLSLILVVGVALAVVAVVNMNKSGSLDTENMSPKMKAIASICSQTDYQKECQ 60
           MVGK  VS LSLILVVG+ALAVVA +N     +   E +SPKMKAI++ICS TDYQ+EC+
Sbjct: 1   MVGKVIVSALSLILVVGIALAVVATINKRNGSNGGKEEVSPKMKAISAICSTTDYQEECK 60

Query: 61  DTLGVLAKNSTSDDPKEYIKTAIMATVDEIKKGFNLTDSLMVEAGNSAFMKMSVEDCKDL 120
           +TL  +A+N++S+DPK+Y K AI+AT+ EI KG+NL+DSL+VEAGN+A +KMSVEDC DL
Sbjct: 61  NTLNHVAQNTSSNDPKDYAKAAILATIKEITKGYNLSDSLIVEAGNNASIKMSVEDCNDL 120

Query: 121 LRFAVRELQASYSSVGANDERSQEERVSDINTWLSAVISYQESCLDALGESNPKLKETMG 180
           L+FA+ ELQASYS+VG +D  +  +R++DI  WLSAV+SYQ+SCLD LGE +P+LK+ M 
Sbjct: 121 LQFAIDELQASYSTVGESDLHTDADRIADIKNWLSAVVSYQQSCLDGLGEFDPQLKQRMQ 180

Query: 181 AGSLDFATKLTSNALAIFSEVSKILGNYGLQLKAPQPNGRRLLGHTEVGSDGFPTWFSKA 240
            G LD A KLTSNALAI + VS IL NYGLQLK  QP+GRRLLG TEV SDGFPTW + A
Sbjct: 181 DG-LDVAGKLTSNALAILTAVSNILDNYGLQLKV-QPSGRRLLGTTEVDSDGFPTWLTGA 240

Query: 241 DRKLLAAARRGGGGVVGVKPNVVVAKDGSGNYKTIGAALAAAPKNSKTRYVIYVKAGIYD 300
           DRKLL A+++GG   V   PN VVAKDGSG YKTI AALAA PK  + RYVIYVKAGIYD
Sbjct: 241 DRKLL-ASKQGG---VRPTPNAVVAKDGSGQYKTIAAALAAYPKALRGRYVIYVKAGIYD 300

Query: 301 EYVTVTSDLTYIFMYGDGPRKTIVTGRKSARDGINTMNTATFAAVGFHFLCKAMGFQNTA 360
           EY+TVT D+  +FMYGDGPRKTIVTGRKS RDG  T NTA+FAA+G  FLCK+MGF NTA
Sbjct: 301 EYITVTKDMKNVFMYGDGPRKTIVTGRKSNRDGFTTQNTASFAAIGEGFLCKSMGFTNTA 360

Query: 361 GPEGHQAVALRVQSDRSAFYECRIDGYQDSLYAQTNRQFYRNCVISGTVDFIFGESSTII 420
           GP+GHQAVALRVQSDRSAF+ CR+DG+QD+LY QT+RQFYRNCVISGTVDFIFG+SSTII
Sbjct: 361 GPQGHQAVALRVQSDRSAFFNCRMDGHQDTLYVQTHRQFYRNCVISGTVDFIFGDSSTII 420

Query: 421 QNSLIIVRRPMQGQQNTVTADGRKENRELSGLVIHNCRIVPEQKLFADRFKIPTFLGRPW 480
           QNSLIIVRRPM  QQNTVTA GR E +E++GLVIHNCRIVPEQKLFA+RFKIPTFLGRPW
Sbjct: 421 QNSLIIVRRPMDNQQNTVTAQGRAEQKEITGLVIHNCRIVPEQKLFAERFKIPTFLGRPW 480

Query: 481 KKYALTVIMESTLGDLIQPAGYMPWEED-SRDTCQYLEYANRGPGANTARRVKWKGVRVI 540
           K+YA TVIMESTLGD IQPAGYMPW  + + +TC YLEY NRGPGANT RRV+WKGV+VI
Sbjct: 481 KQYARTVIMESTLGDFIQPAGYMPWSGNFALETCLYLEYGNRGPGANTNRRVRWKGVKVI 540

Query: 541 GRNEALKFTVGSPFLIGNVWLPETRGKFLPGLKN 574
           GRNEAL++T G+ FL+G  WLP T G +  GLKN
Sbjct: 541 GRNEALQYTAGA-FLLGRTWLPRTGGIYYLGLKN 567

BLAST of Moc04g28790 vs. NCBI nr
Match: KGN49779.1 (hypothetical protein Csa_017783 [Cucumis sativus])

HSP 1 Score: 795.4 bits (2053), Expect = 3.2e-226
Identity = 413/574 (71.95%), Postives = 475/574 (82.75%), Query Frame = 0

Query: 1   MVGKAAVSVLSLILVVGVALAVVAVVNMNKSGSLDTENMSPKMKAIASICSQTDYQKECQ 60
           MVGK  +S +SLILVVGVALAVVA+VN + S S +T N+SPKMKA+ASICS  DYQ ECQ
Sbjct: 1   MVGKVVMSGISLILVVGVALAVVAIVNKSDS-SDETANLSPKMKAVASICSNADYQPECQ 60

Query: 61  DTLGVLAKNSTSDDPKEYIKTAIMATVDEIKKGFNLTDSLMVEAGNSAFMKMSVEDCKDL 120
            TLG +  NS+ +DPKE+IK AI +T++E+KKG+NLTD+LMVEA N+A +KMSV+DCKDL
Sbjct: 61  TTLGSVGNNSSVEDPKEFIKAAITSTIEEMKKGYNLTDNLMVEAANNATIKMSVDDCKDL 120

Query: 121 LRFAVRELQASYSSVGANDERSQEERVSDINTWLSAVISYQESCLDALGESNPKLKETMG 180
           L+ A+ EL ASYS+VG  D  + E+R++DI  WL+AVISYQ+SCLD L E +P+LK+ M 
Sbjct: 121 LQSAIDELHASYSTVGDPDLHTNEDRIADIKNWLTAVISYQQSCLDGLEEFDPQLKQKMQ 180

Query: 181 AGSLDFATKLTSNALAIFSEVSKILGNYGLQLKAPQPNGRRLLGHTEVGSDGFPTWFSKA 240
            G LD A KLTSNALAI   VS IL   GLQLK  QP+GRRLLG TEV SD FPTW + +
Sbjct: 181 DG-LDVAGKLTSNALAIVGAVSDILKELGLQLKV-QPSGRRLLGTTEVDSDSFPTWLTGS 240

Query: 241 DRKLLAAARRGGGGVVGVKPNVVVAKDGSGNYKTIGAALAAAPKNSKTRYVIYVKAGIYD 300
           DRKLL AA+RGG   V VKPNVVVAKDGSG YKTIGAALAA PK  K RYVIYVKAG+Y+
Sbjct: 241 DRKLL-AAKRGG---VRVKPNVVVAKDGSGQYKTIGAALAAYPKALKGRYVIYVKAGVYN 300

Query: 301 EYVTVTSDLTYIFMYGDGPRKTIVTGRKSARDGINTMNTATFAAVGFHFLCKAMGFQNTA 360
           E + VT D+  IFMYGDGPRKTIVTGRKS RDGI T NTA+FAA+G  FLCK+MGF NTA
Sbjct: 301 EPIIVTKDMKNIFMYGDGPRKTIVTGRKSNRDGITTQNTASFAAIGEGFLCKSMGFTNTA 360

Query: 361 GPEGHQAVALRVQSDRSAFYECRIDGYQDSLYAQTNRQFYRNCVISGTVDFIFGESSTII 420
           GPEGHQAVALRVQSDRSAF+ CR+DGYQD+LY QT+RQFYRNCVISGTVDFIFG+S+TII
Sbjct: 361 GPEGHQAVALRVQSDRSAFFNCRMDGYQDTLYVQTHRQFYRNCVISGTVDFIFGDSNTII 420

Query: 421 QNSLIIVRRPMQGQQNTVTADGRKENRELSGLVIHNCRIVPEQKLFADRFKIPTFLGRPW 480
           QNSLIIVRRPM  QQNTVTA+GR + +E+SGLVIHNCRIVPEQKLFA+RFKIPTFLGRPW
Sbjct: 421 QNSLIIVRRPMDNQQNTVTANGRADKKEISGLVIHNCRIVPEQKLFAERFKIPTFLGRPW 480

Query: 481 KKYALTVIMESTLGDLIQPAGYMPWEED-SRDTCQYLEYANRGPGANTARRVKWKGVRVI 540
           K+YA TVIMESTLGD IQPAGYMPW  + +  TC Y EY NRGPGANT RRV+WKGVRVI
Sbjct: 481 KEYARTVIMESTLGDFIQPAGYMPWSGNFALATCSYFEYGNRGPGANTNRRVRWKGVRVI 540

Query: 541 GRNEALKFTVGSPFLIGNVWLPETRGKFLPGLKN 574
           GRNEA++FT G PFL+G  WLP T G +L GLKN
Sbjct: 541 GRNEAMQFTAG-PFLLGKAWLPGTGGPYLLGLKN 566

BLAST of Moc04g28790 vs. ExPASy Swiss-Prot
Match: Q3E8Z8 (Putative pectinesterase/pectinesterase inhibitor 28 OS=Arabidopsis thaliana OX=3702 GN=PME28 PE=2 SV=1)

HSP 1 Score: 433.3 bits (1113), Expect = 4.2e-120
Identity = 230/563 (40.85%), Postives = 346/563 (61.46%), Query Frame = 0

Query: 10  LSLILVVGVALAVVAVVNMNKSGSLDTENMSPKMKAIASICSQTDYQKECQDTLGVLAKN 69
           +S +L++ + +AV   V++NKS +   E ++  +KAI  +C+ TDY++ C+DTL   AK+
Sbjct: 21  ISSVLLISMVVAVTIGVSVNKSDNAGDEEITTSVKAIKDVCAPTDYKETCEDTLRKDAKD 80

Query: 70  STSDDPKEYIKTAIMATVDEIKKGFNLTDSLMVEAGNSAFMKMSVEDCKDLLRFAVRELQ 129
           ++  DP E +KTA  AT+ +I      + + M+E       KM+++ CK+L+ +A+ EL 
Sbjct: 81  TS--DPLELVKTAFNATMKQISDVAKKSQT-MIELQKDPRAKMALDQCKELMDYAIGELS 140

Query: 130 ASYSSVGANDERSQEERVSDINTWLSAVISYQESCLDALGESNPKLKETMGAGSLDFATK 189
            S+  +G  +    +E +  +  WLSA IS++++CLD    +     ET+   +L  A +
Sbjct: 141 KSFEELGKFEFHKVDEALVKLRIWLSATISHEQTCLDGFQGTQGNAGETI-KKALKTAVQ 200

Query: 190 LTSNALAIFSEVSKILGNYGLQLKAPQPNGRRLLGHTEVGSDGFPTWFSKADRKLLAAAR 249
           LT N LA+ +E+S  LG    Q++ P+ N RRLL      S  FP+W     R+LL A  
Sbjct: 201 LTHNGLAMVTEMSNYLG----QMQIPEMNSRRLL------SQEFPSWMDARARRLLNAP- 260

Query: 250 RGGGGVVGVKPNVVVAKDGSGNYKTIGAALAAAPKNSKTRYVIYVKAGIYDEYVTVTSDL 309
                +  VKP++VVA+DGSG YKTI  AL   PK   T +V+++K GIY EYV V   +
Sbjct: 261 -----MSEVKPDIVVAQDGSGQYKTINEALNFVPKKKNTTFVVHIKEGIYKEYVQVNRSM 320

Query: 310 TYIFMYGDGPRKTIVTGRKSARDGINTMNTATFAAVGFHFLCKAMGFQNTAGPEGHQAVA 369
           T++   GDGP KT+++G KS +DGI T  TAT A VG HF+ K + F+NTAG   HQAVA
Sbjct: 321 THLVFIGDGPDKTVISGSKSYKDGITTYKTATVAIVGDHFIAKNIAFENTAGAIKHQAVA 380

Query: 370 LRVQSDRSAFYECRIDGYQDSLYAQTNRQFYRNCVISGTVDFIFGESSTIIQNSLIIVRR 429
           +RV +D S FY C+ DGYQD+LYA ++RQFYR+C ISGT+DF+FG+++ + QN  ++VR+
Sbjct: 381 IRVLADESIFYNCKFDGYQDTLYAHSHRQFYRDCTISGTIDFLFGDAAAVFQNCTLLVRK 440

Query: 430 PMQGQQNTVTADGRKENRELSGLVIHNCRIVPEQKLFADRFKIPTFLGRPWKKYALTVIM 489
           P+  Q   +TA GRK+ RE +G V+  C IV E    A + +  T+LGRPWK+Y+ T+IM
Sbjct: 441 PLLNQACPITAHGRKDPRESTGFVLQGCTIVGEPDYLAVKEQSKTYLGRPWKEYSRTIIM 500

Query: 490 ESTLGDLIQPAGYMPW-EEDSRDTCQYLEYANRGPGANTARRVKWKGVRVIGRNEALKFT 549
            + + D + P G+ PW  E   +T  Y E  N GPGA   +RV W G++ +   E LKFT
Sbjct: 501 NTFIPDFVPPEGWQPWLGEFGLNTLFYSEVQNTGPGAAITKRVTWPGIKKLSDEEILKFT 560

Query: 550 VGSPFLIGNVWLPETRGKFLPGL 572
             + ++ G+ W+P     ++ GL
Sbjct: 561 -PAQYIQGDAWIPGKGVPYILGL 562

BLAST of Moc04g28790 vs. ExPASy Swiss-Prot
Match: Q8GX86 (Probable pectinesterase/pectinesterase inhibitor 21 OS=Arabidopsis thaliana OX=3702 GN=PME21 PE=2 SV=2)

HSP 1 Score: 427.9 bits (1099), Expect = 1.8e-118
Identity = 231/566 (40.81%), Postives = 345/566 (60.95%), Query Frame = 0

Query: 7   VSVLSLILVVGVALAVVAVVNMNK--SGSLDTENMSPKMKAIASICSQTDYQKECQDTLG 66
           V  +S +L++ + +AV   V++NK    S     ++  +KA+  +C+ TDY+K C+DTL 
Sbjct: 17  VITISSVLLISMVVAVTVGVSLNKHDGDSKGKAEVNASVKAVKDVCAPTDYRKTCEDTLI 76

Query: 67  VLAKNSTSDDPKEYIKTAIMATVDEIKKGFNLTDSLMVEAGNSAFMKMSVEDCKDLLRFA 126
              KN+T  DP E +KTA   T+ +I      + ++M E    +  +M+++ CK+L+ +A
Sbjct: 77  KNGKNTT--DPMELVKTAFNVTMKQITDAAKKSQTIM-ELQKDSRTRMALDQCKELMDYA 136

Query: 127 VRELQASYSSVGANDERSQEERVSDINTWLSAVISYQESCLDALGESNPKLKETMGAGSL 186
           + EL  S+  +G  +    +E + ++  WLSA IS++E+CL+    +     ETM   +L
Sbjct: 137 LDELSNSFEELGKFEFHLLDEALINLRIWLSAAISHEETCLEGFQGTQGNAGETM-KKAL 196

Query: 187 DFATKLTSNALAIFSEVSKILGNYGLQLKAPQPNGRRLLGHTEVGSDGFPTWFSKADRKL 246
             A +LT N LAI SE+S  +G    Q++ P  N RRLL      ++GFP+W  +  RKL
Sbjct: 197 KTAIELTHNGLAIISEMSNFVG----QMQIPGLNSRRLL------AEGFPSWVDQRGRKL 256

Query: 247 LAAARRGGGGVVGVKPNVVVAKDGSGNYKTIGAALAAAPKNSKTRYVIYVKAGIYDEYVT 306
           L AA         VKP++VVA+DGSG YKTI  AL   PK   T +V+++KAG+Y EYV 
Sbjct: 257 LQAA----AAYSDVKPDIVVAQDGSGQYKTINEALQFVPKKRNTTFVVHIKAGLYKEYVQ 316

Query: 307 VTSDLTYIFMYGDGPRKTIVTGRKSARDGINTMNTATFAAVGFHFLCKAMGFQNTAGPEG 366
           V   ++++   GDGP KTI++G K+ +DGI T  TAT A VG +F+ K +GF+NTAG   
Sbjct: 317 VNKTMSHLVFIGDGPDKTIISGNKNYKDGITTYRTATVAIVGNYFIAKNIGFENTAGAIK 376

Query: 367 HQAVALRVQSDRSAFYECRIDGYQDSLYAQTNRQFYRNCVISGTVDFIFGESSTIIQNSL 426
           HQAVA+RVQSD S F+ CR DGYQD+LY  ++RQF+R+C ISGT+DF+FG+++ + QN  
Sbjct: 377 HQAVAVRVQSDESIFFNCRFDGYQDTLYTHSHRQFFRDCTISGTIDFLFGDAAAVFQNCT 436

Query: 427 IIVRRPMQGQQNTVTADGRKENRELSGLVIHNCRIVPEQKLFADRFKIPTFLGRPWKKYA 486
           ++VR+P+  Q   +TA GRK+ RE +G V   C I  E    A +     +LGRPWK+Y+
Sbjct: 437 LLVRKPLPNQACPITAHGRKDPRESTGFVFQGCTIAGEPDYLAVKETSKAYLGRPWKEYS 496

Query: 487 LTVIMESTLGDLIQPAGYMPWEED-SRDTCQYLEYANRGPGANTARRVKWKGVRVIGRNE 546
            T+IM + + D +QP G+ PW  D    T  Y E  N GPG+  A RV W G++ +   +
Sbjct: 497 RTIIMNTFIPDFVQPQGWQPWLGDFGLKTLFYSEVQNTGPGSALANRVTWAGIKTLSEED 556

Query: 547 ALKFTVGSPFLIGNVWLPETRGKFLP 570
            LKFT  + ++ G+ W+P   GK +P
Sbjct: 557 ILKFT-PAQYIQGDDWIP---GKGVP 560

BLAST of Moc04g28790 vs. ExPASy Swiss-Prot
Match: O80722 (Pectinesterase 4 OS=Arabidopsis thaliana OX=3702 GN=PME4 PE=2 SV=1)

HSP 1 Score: 414.5 bits (1064), Expect = 2.0e-114
Identity = 247/570 (43.33%), Postives = 342/570 (60.00%), Query Frame = 0

Query: 1   MVGKAAVSVLSLILVVGVALAVVAVVNMNKSGSLDTENMSPKMKAIASICSQTDYQKECQ 60
           M+GK  VSV S++L+VGVA+ VVA +N N        N+SP+MKA+  IC  T  +  C 
Sbjct: 1   MIGKVVVSVASILLIVGVAIGVVAFINKNGDA-----NLSPQMKAVQGICQSTSDKASCV 60

Query: 61  DTLGVLAKNSTSDDPKEYIKTAIMATVDEIKKGFNLTDSLMVEAGNSAF--MKMSVEDCK 120
            TL    +   S+DP + IK  ++AT DE+ K  N T    V  G+S     K  ++ CK
Sbjct: 61  KTL----EPVKSEDPNKLIKAFMLATKDELTKSSNFTGQTEVNMGSSISPNNKAVLDYCK 120

Query: 121 DLLRFAVRELQASYSSVGANDERSQEERVSDINTWLSAVISYQESCLDALGESNPKLKET 180
            +  +A+ +L      +G  D      ++  +  WL  V +YQ  CLD + E +  L++ 
Sbjct: 121 RVFMYALEDLATIIEEMG-EDLSQIGSKIDQLKQWLIGVYNYQTDCLDDIEEDD--LRKA 180

Query: 181 MGAGSLDFATKLTSNALAIF----SEVSKI------LGNYGLQLKAP------------Q 240
           +G G  + +  LT+NA+ IF    S ++KI      L N    +  P             
Sbjct: 181 IGEGIAN-SKILTTNAIDIFHTVVSAMAKINNKVDDLKNMTGGIPTPGAPPVVDESPVAD 240

Query: 241 PNG--RRLLGHTEVGSDGFPTWFSKADRKLLAAA---RRGG-GGVVGVKPNVVVAKDGSG 300
           P+G  RRLL   ++   G PTW S ADRKL+A A   RRGG GG   V+ N VVAKDGSG
Sbjct: 241 PDGPARRLL--EDIDETGIPTWVSGADRKLMAKAGRGRRGGRGGGARVRTNFVVAKDGSG 300

Query: 301 NYKTIGAALAAAPKNSKTRYVIYVKAGIYDEYVTVTSDLTYIFMYGDGPRKTIVTGRKSA 360
            +KT+  A+ A P+N++ R +IY+KAG+Y E V +      IFM+GDG RKT+++  +S 
Sbjct: 301 QFKTVQQAVDACPENNRGRCIIYIKAGLYREQVIIPKKKNNIFMFGDGARKTVISYNRSV 360

Query: 361 --RDGINTMNTATFAAVGFHFLCKAMGFQNTAGPEGHQAVALRVQSDRSAFYECRIDGYQ 420
               G  T  +AT       F+ K MGF+NTAGP GHQA A+RV  DR+  + CR DGYQ
Sbjct: 361 ALSRGTTTSLSATVQVESEGFMAKWMGFKNTAGPMGHQAAAIRVNGDRAVIFNCRFDGYQ 420

Query: 421 DSLYAQTNRQFYRNCVISGTVDFIFGESSTIIQNSLIIVRRPMQGQQNTVTADGRKENRE 480
           D+LY    RQFYRNCV+SGTVDFIFG+S+T+IQN+LI+VR+  +GQ NTVTADG +    
Sbjct: 421 DTLYVNNGRQFYRNCVVSGTVDFIFGKSATVIQNTLIVVRKGSKGQYNTVTADGNELGLG 480

Query: 481 LS-GLVIHNCRIVPEQKLFADRFKIPTFLGRPWKKYALTVIMESTLGDLIQPAGYMPWEE 537
           +  G+V+ NCRIVP++KL  +R  + T+LGRPWKK++ TVIM + +GDLI+P G+  W+ 
Sbjct: 481 MKIGIVLQNCRIVPDRKLTPERLTVATYLGRPWKKFSTTVIMSTEMGDLIRPEGWKIWDG 540

BLAST of Moc04g28790 vs. ExPASy Swiss-Prot
Match: Q9SMY6 (Putative pectinesterase/pectinesterase inhibitor 45 OS=Arabidopsis thaliana OX=3702 GN=PME45 PE=2 SV=1)

HSP 1 Score: 411.8 bits (1057), Expect = 1.3e-113
Identity = 238/553 (43.04%), Postives = 330/553 (59.67%), Query Frame = 0

Query: 27  NMNKSGSLDTENMSPKM--------------KAIASICSQTDYQKECQDTL-GVLAKNST 86
           N +K     +E+ SPK               K I ++C+ T Y+  CQ+TL     K++ 
Sbjct: 62  NKSKDSPTKSESPSPKPPSSAAQTVKAGQVDKIIQTLCNSTLYKPTCQNTLKNETKKDTP 121

Query: 87  SDDPKEYIKTAIMATVDEIKKGFNLTDSLMVEAGNSAFMKMSVEDCKDLLRFAVRELQAS 146
             DP+  +K+AI+A  D++ + F    SL  E  +    K ++  CK L+  A  EL  S
Sbjct: 122 QTDPRSLLKSAIVAVNDDLDQVFKRVLSLKTENKDD---KDAIAQCKLLVDEAKEELGTS 181

Query: 147 YSSVGANDERSQEERVSDINTWLSAVISYQESCLDALGESNPKLKETMGAGSLDFATKLT 206
              +  ++  +  + V D+++WLSAV+SYQE+C+D   E   KLK T    + + +  LT
Sbjct: 182 MKRINDSEVNNFAKIVPDLDSWLSAVMSYQETCVDGFEEG--KLK-TEIRKNFNSSQVLT 241

Query: 207 SNALAIFSEVSKILGNYGLQLKAPQPNGRRLL--GHTEVGSDGFPTWFSKADRKLLAAAR 266
           SN+LA+   +   L +       P+   R LL    +   +D   +W S  +R++L A  
Sbjct: 242 SNSLAMIKSLDGYLSS------VPKVKTRLLLEARSSAKETDHITSWLSNKERRMLKAV- 301

Query: 267 RGGGGVVGVKPNVVVAKDGSGNYKTIGAALAAAPKNSKTRYVIYVKAGIYDEYVTVTSDL 326
                V  +KPN  VAKDGSGN+ TI AAL A P   + RY IY+K GIYDE V +    
Sbjct: 302 ----DVKALKPNATVAKDGSGNFTTINAALKAMPAKYQGRYTIYIKHGIYDESVIIDKKK 361

Query: 327 TYIFMYGDGPRKTIVTGRKSARDGINTMNTATFAAVGFHFLCKAMGFQNTAGPEGHQAVA 386
             + M GDG +KTIVTG KS    I T  TATF A G  F+ ++MGF+NTAGPEGHQAVA
Sbjct: 362 PNVTMVGDGSQKTIVTGNKSHAKKIRTFLTATFVAQGEGFMAQSMGFRNTAGPEGHQAVA 421

Query: 387 LRVQSDRSAFYECRIDGYQDSLYAQTNRQFYRNCVISGTVDFIFGESSTIIQNSLIIVRR 446
           +RVQSDRS F  CR +GYQD+LYA T+RQ+YR+CVI GTVDFIFG+++ I QN  I +R+
Sbjct: 422 IRVQSDRSVFLNCRFEGYQDTLYAYTHRQYYRSCVIIGTVDFIFGDAAAIFQNCDIFIRK 481

Query: 447 PMQGQQNTVTADGRKENRELSGLVIHNCRIVPEQKLFADRFKIPTFLGRPWKKYALTVIM 506
            + GQ+NTVTA GR +  + +G VIHNC + P + L   + +  ++LGRPWK ++ TV+M
Sbjct: 482 GLPGQKNTVTAQGRVDKFQTTGFVIHNCTVAPNEDLKPVKAQFKSYLGRPWKPHSRTVVM 541

Query: 507 ESTLGDLIQPAGYMPWEED--SRDTCQYLEYANRGPGANTARRVKWKGVRVIGRNEALKF 561
           EST+ D+I P G++ W+E   + DT  Y EY N GP   TA RVKW G RV+ + EA+KF
Sbjct: 542 ESTIEDVIDPVGWLRWQETDFAIDTLSYAEYKNDGPSGATAARVKWPGFRVLNKEEAMKF 595

BLAST of Moc04g28790 vs. ExPASy Swiss-Prot
Match: Q7Y201 (Probable pectinesterase/pectinesterase inhibitor 13 OS=Arabidopsis thaliana OX=3702 GN=PME13 PE=2 SV=2)

HSP 1 Score: 409.8 bits (1052), Expect = 4.9e-113
Identity = 233/524 (44.47%), Postives = 321/524 (61.26%), Query Frame = 0

Query: 44  KAIASICSQTDYQKECQDTLGVLAKNSTS-----DDPKEYIKTAIMATVDEIKKGFNLTD 103
           K I ++CS T Y + C+ TL    KN T      D+P  ++K+AI A  +++        
Sbjct: 107 KIIQTLCSSTLYMQICEKTL----KNRTDKGFALDNPTTFLKSAIEAVNEDLDLVLEKVL 166

Query: 104 SLMVEAGNSAFMKMSVEDCKDLLRFAVRELQASYSSVGANDERSQEERVSDINTWLSAVI 163
           SL  E  +    K ++E CK L+  A  E  AS + +   +  S E+ V D+ +WLSAV+
Sbjct: 167 SLKTENQDD---KDAIEQCKLLVEDAKEETVASLNKINVTEVNSFEKVVPDLESWLSAVM 226

Query: 164 SYQESCLDALGESNPKLKETMGAGSLDFATKLTSNALAIFSEVSKILGNYGLQLKAPQPN 223
           SYQE+CLD   E N K +      S++ +  LTSN+LA+    ++   N    +K  +  
Sbjct: 227 SYQETCLDGFEEGNLKSEVKT---SVNSSQVLTSNSLALIKTFTE---NLSPVMKVVE-- 286

Query: 224 GRRLLGHTEVGSDGFPTWFSKADRKLLAAARRGGGGVVGVKPNVVVAKDGSGNYKTIGAA 283
            R LL       D  P+W S  DR++L A       V  +KPN  VAKDGSG++ TI  A
Sbjct: 287 -RHLL-------DDIPSWVSNDDRRMLRAV-----DVKALKPNATVAKDGSGDFTTINDA 346

Query: 284 LAAAPKNSKTRYVIYVKAGIYDEYVTVTSDLTYIFMYGDGPRKTIVTGRKSARDGINTMN 343
           L A P+  + RY+IYVK GIYDEYVTV      + M GDG +KTIVTG KS    I T  
Sbjct: 347 LRAMPEKYEGRYIIYVKQGIYDEYVTVDKKKANLTMVGDGSQKTIVTGNKSHAKKIRTFL 406

Query: 344 TATFAAVGFHFLCKAMGFQNTAGPEGHQAVALRVQSDRSAFYECRIDGYQDSLYAQTNRQ 403
           TATF A G  F+ ++MGF+NTAGPEGHQAVA+RVQSDRS F  CR +GYQD+LYA T+RQ
Sbjct: 407 TATFVAQGEGFMAQSMGFRNTAGPEGHQAVAIRVQSDRSIFLNCRFEGYQDTLYAYTHRQ 466

Query: 404 FYRNCVISGTVDFIFGESSTIIQNSLIIVRRPMQGQQNTVTADGRKENRELSGLVIHNCR 463
           +YR+CVI GT+DFIFG+++ I QN  I +R+ + GQ+NTVTA GR +  + +G V+HNC+
Sbjct: 467 YYRSCVIVGTIDFIFGDAAAIFQNCNIFIRKGLPGQKNTVTAQGRVDKFQTTGFVVHNCK 526

Query: 464 IVPEQKLFADRFKIPTFLGRPWKKYALTVIMESTLGDLIQPAGYMPWEED--SRDTCQYL 523
           I   + L   + +  ++LGRPWK Y+ T+IMES + ++I P G++ W+E   + DT  Y 
Sbjct: 527 IAANEDLKPVKEEYKSYLGRPWKNYSRTIIMESKIENVIDPVGWLRWQETDFAIDTLYYA 586

Query: 524 EYANRGPGANTARRVKWKGVRVIGRNEALKFTVGSPFLIGNVWL 561
           EY N+G   +T  RVKW G +VI + EAL +TVG PFL G+ W+
Sbjct: 587 EYNNKGSSGDTTSRVKWPGFKVINKEEALNYTVG-PFLQGD-WI 600

BLAST of Moc04g28790 vs. ExPASy TrEMBL
Match: A0A6J1CZA2 (Pectinesterase OS=Momordica charantia OX=3673 GN=LOC111015559 PE=3 SV=1)

HSP 1 Score: 1133.6 bits (2931), Expect = 0.0e+00
Identity = 573/573 (100.00%), Postives = 573/573 (100.00%), Query Frame = 0

Query: 1   MVGKAAVSVLSLILVVGVALAVVAVVNMNKSGSLDTENMSPKMKAIASICSQTDYQKECQ 60
           MVGKAAVSVLSLILVVGVALAVVAVVNMNKSGSLDTENMSPKMKAIASICSQTDYQKECQ
Sbjct: 1   MVGKAAVSVLSLILVVGVALAVVAVVNMNKSGSLDTENMSPKMKAIASICSQTDYQKECQ 60

Query: 61  DTLGVLAKNSTSDDPKEYIKTAIMATVDEIKKGFNLTDSLMVEAGNSAFMKMSVEDCKDL 120
           DTLGVLAKNSTSDDPKEYIKTAIMATVDEIKKGFNLTDSLMVEAGNSAFMKMSVEDCKDL
Sbjct: 61  DTLGVLAKNSTSDDPKEYIKTAIMATVDEIKKGFNLTDSLMVEAGNSAFMKMSVEDCKDL 120

Query: 121 LRFAVRELQASYSSVGANDERSQEERVSDINTWLSAVISYQESCLDALGESNPKLKETMG 180
           LRFAVRELQASYSSVGANDERSQEERVSDINTWLSAVISYQESCLDALGESNPKLKETMG
Sbjct: 121 LRFAVRELQASYSSVGANDERSQEERVSDINTWLSAVISYQESCLDALGESNPKLKETMG 180

Query: 181 AGSLDFATKLTSNALAIFSEVSKILGNYGLQLKAPQPNGRRLLGHTEVGSDGFPTWFSKA 240
           AGSLDFATKLTSNALAIFSEVSKILGNYGLQLKAPQPNGRRLLGHTEVGSDGFPTWFSKA
Sbjct: 181 AGSLDFATKLTSNALAIFSEVSKILGNYGLQLKAPQPNGRRLLGHTEVGSDGFPTWFSKA 240

Query: 241 DRKLLAAARRGGGGVVGVKPNVVVAKDGSGNYKTIGAALAAAPKNSKTRYVIYVKAGIYD 300
           DRKLLAAARRGGGGVVGVKPNVVVAKDGSGNYKTIGAALAAAPKNSKTRYVIYVKAGIYD
Sbjct: 241 DRKLLAAARRGGGGVVGVKPNVVVAKDGSGNYKTIGAALAAAPKNSKTRYVIYVKAGIYD 300

Query: 301 EYVTVTSDLTYIFMYGDGPRKTIVTGRKSARDGINTMNTATFAAVGFHFLCKAMGFQNTA 360
           EYVTVTSDLTYIFMYGDGPRKTIVTGRKSARDGINTMNTATFAAVGFHFLCKAMGFQNTA
Sbjct: 301 EYVTVTSDLTYIFMYGDGPRKTIVTGRKSARDGINTMNTATFAAVGFHFLCKAMGFQNTA 360

Query: 361 GPEGHQAVALRVQSDRSAFYECRIDGYQDSLYAQTNRQFYRNCVISGTVDFIFGESSTII 420
           GPEGHQAVALRVQSDRSAFYECRIDGYQDSLYAQTNRQFYRNCVISGTVDFIFGESSTII
Sbjct: 361 GPEGHQAVALRVQSDRSAFYECRIDGYQDSLYAQTNRQFYRNCVISGTVDFIFGESSTII 420

Query: 421 QNSLIIVRRPMQGQQNTVTADGRKENRELSGLVIHNCRIVPEQKLFADRFKIPTFLGRPW 480
           QNSLIIVRRPMQGQQNTVTADGRKENRELSGLVIHNCRIVPEQKLFADRFKIPTFLGRPW
Sbjct: 421 QNSLIIVRRPMQGQQNTVTADGRKENRELSGLVIHNCRIVPEQKLFADRFKIPTFLGRPW 480

Query: 481 KKYALTVIMESTLGDLIQPAGYMPWEEDSRDTCQYLEYANRGPGANTARRVKWKGVRVIG 540
           KKYALTVIMESTLGDLIQPAGYMPWEEDSRDTCQYLEYANRGPGANTARRVKWKGVRVIG
Sbjct: 481 KKYALTVIMESTLGDLIQPAGYMPWEEDSRDTCQYLEYANRGPGANTARRVKWKGVRVIG 540

Query: 541 RNEALKFTVGSPFLIGNVWLPETRGKFLPGLKN 574
           RNEALKFTVGSPFLIGNVWLPETRGKFLPGLKN
Sbjct: 541 RNEALKFTVGSPFLIGNVWLPETRGKFLPGLKN 573

BLAST of Moc04g28790 vs. ExPASy TrEMBL
Match: A0A6J1CYB2 (Pectinesterase OS=Momordica charantia OX=3673 GN=LOC111015570 PE=3 SV=1)

HSP 1 Score: 917.9 bits (2371), Expect = 2.1e-263
Identity = 468/573 (81.68%), Postives = 512/573 (89.35%), Query Frame = 0

Query: 2   VGKAAVSVLSLILVVGVALAVVAVVNMNKSGSLDTENMSPKMKAIASICSQTDYQKECQD 61
           +G A VSVLSLILVVGVALAVVAVVN N  GS D EN+SPKMKA+A+ICS TDYQ+ECQ+
Sbjct: 1   MGTAVVSVLSLILVVGVALAVVAVVNRN-HGSFDGENLSPKMKAVATICSHTDYQEECQN 60

Query: 62  TLGVLAKNSTSDDPKEYIKTAIMATVDEIKKGFNLTDSLMVEAGNSAFMKMSVEDCKDLL 121
           TLG +A NS+SDDPKEYIK+AIMATV+E KKGF LTDSLMVEAGN++FMKMSVEDCKDLL
Sbjct: 61  TLGSIAHNSSSDDPKEYIKSAIMATVEEFKKGFKLTDSLMVEAGNNSFMKMSVEDCKDLL 120

Query: 122 RFAVRELQASYSSVGANDERSQEERVSDINTWLSAVISYQESCLDALGESNPKLKETMGA 181
           +FAV ELQASYSSVG +DERSQEERV+DINTWLSAVISYQ+SCLD LGE+NPKLKETM  
Sbjct: 121 QFAVEELQASYSSVGDSDERSQEERVADINTWLSAVISYQQSCLDGLGEANPKLKETM-E 180

Query: 182 GSLDFATKLTSNALAIFSEVSKILGNYGLQLKAPQPNGRRLLGHTEVGSDGFPTWFSKAD 241
           G +D A KLTSNALAI ++VS++LG+YGLQLK  QP GRRLLG+TEVGSDGFPTW S AD
Sbjct: 181 GGIDIARKLTSNALAIVADVSQVLGDYGLQLKV-QPTGRRLLGNTEVGSDGFPTWLSGAD 240

Query: 242 RKLLAAARRGGGGVVGVKPNVVVAKDGSGNYKTIGAALAAAPKNSKTRYVIYVKAGIYDE 301
           R+LLAAA+ GGGG   V PN VVAKDGSG YKTI AALAAAPKNSKTR+VIYVKAGIY+E
Sbjct: 241 RRLLAAAKGGGGG--KVTPNAVVAKDGSGQYKTIAAALAAAPKNSKTRHVIYVKAGIYNE 300

Query: 302 YVTVTSDLTYIFMYGDGPRKTIVTGRKSARDGINTMNTATFAAVGFHFLCKAMGFQNTAG 361
           ++ VT D T IFMYGDGPRKTIVTGRKS RDGI TMNTATF+AVG  FLCKAMGFQNTAG
Sbjct: 301 FIIVTKDYTNIFMYGDGPRKTIVTGRKSFRDGITTMNTATFSAVGNGFLCKAMGFQNTAG 360

Query: 362 PEGHQAVALRVQSDRSAFYECRIDGYQDSLYAQTNRQFYRNCVISGTVDFIFGESSTIIQ 421
           PEGHQAVALRVQSDRSAF+ CR+DGYQD+LY   NRQFYRNCVISGTVDFIFG+S+TIIQ
Sbjct: 361 PEGHQAVALRVQSDRSAFFNCRMDGYQDTLYVHANRQFYRNCVISGTVDFIFGDSNTIIQ 420

Query: 422 NSLIIVRRPMQGQQNTVTADGRKENRELSGLVIHNCRIVPEQKLFADRFKIPTFLGRPWK 481
           NSLIIVRRPM  QQNTVTA+GRKEN+E SGLVIHNCRIVPEQKLFADRFKIPTFLGRPWK
Sbjct: 421 NSLIIVRRPMDNQQNTVTANGRKENKEFSGLVIHNCRIVPEQKLFADRFKIPTFLGRPWK 480

Query: 482 KYALTVIMESTLGDLIQPAGYMPWEED-SRDTCQYLEYANRGPGANTARRVKWKGVRVIG 541
           +YALTVIME+TLGD IQPAGYMPW+   + DTCQYLEYANRGPGANTARRVKWKGVRV+G
Sbjct: 481 EYALTVIMETTLGDFIQPAGYMPWQGTFALDTCQYLEYANRGPGANTARRVKWKGVRVMG 540

Query: 542 RNEALKFTVGSPFLIGNVWLPETRGKFLPGLKN 574
           RNEALKFT GSPFL+G  WLP T G FL GLKN
Sbjct: 541 RNEALKFTAGSPFLLGKEWLPATGGNFLLGLKN 568

BLAST of Moc04g28790 vs. ExPASy TrEMBL
Match: A0A5A7U4V7 (Pectinesterase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold180G00110 PE=3 SV=1)

HSP 1 Score: 800.0 bits (2065), Expect = 6.3e-228
Identity = 408/574 (71.08%), Postives = 477/574 (83.10%), Query Frame = 0

Query: 1   MVGKAAVSVLSLILVVGVALAVVAVVNMNKSGSLDTENMSPKMKAIASICSQTDYQKECQ 60
           MVGK  VS LSLILVVG+ALAVVA +N     +   E +SPKMKAI++ICS TDYQ+EC+
Sbjct: 1   MVGKVIVSALSLILVVGIALAVVATINKRNGSNGGKEEVSPKMKAISAICSTTDYQEECK 60

Query: 61  DTLGVLAKNSTSDDPKEYIKTAIMATVDEIKKGFNLTDSLMVEAGNSAFMKMSVEDCKDL 120
           +TL  +A N++S+DPK+Y K AI+AT+ EI KG+NL+DSL+VEAGN+A +KMSVEDCKDL
Sbjct: 61  NTLNHVAHNTSSNDPKDYAKAAILATIKEITKGYNLSDSLIVEAGNNASIKMSVEDCKDL 120

Query: 121 LRFAVRELQASYSSVGANDERSQEERVSDINTWLSAVISYQESCLDALGESNPKLKETMG 180
           L+FA+ ELQASYS+VG +D  +  +R++DI  WLSAV+SYQ+SCLD LGE +P+LK+ M 
Sbjct: 121 LQFAIDELQASYSTVGESDLHTDADRIADIKNWLSAVVSYQQSCLDGLGEFDPQLKQRMQ 180

Query: 181 AGSLDFATKLTSNALAIFSEVSKILGNYGLQLKAPQPNGRRLLGHTEVGSDGFPTWFSKA 240
            G LD A KLTSNALAI + VS IL NYGLQLK  QP+GRRLLG TEV SDGFPTW + A
Sbjct: 181 DG-LDVAGKLTSNALAIVTAVSNILDNYGLQLKV-QPSGRRLLGTTEVDSDGFPTWLTGA 240

Query: 241 DRKLLAAARRGGGGVVGVKPNVVVAKDGSGNYKTIGAALAAAPKNSKTRYVIYVKAGIYD 300
           DRKLL A+++GG   V   PN VVAKDGSG YKTI AALAA PK  + RYVIYVKAGIYD
Sbjct: 241 DRKLL-ASKQGG---VRPTPNAVVAKDGSGQYKTIAAALAAYPKALRGRYVIYVKAGIYD 300

Query: 301 EYVTVTSDLTYIFMYGDGPRKTIVTGRKSARDGINTMNTATFAAVGFHFLCKAMGFQNTA 360
           EY+TVT D+  +FMYGDGPRKTIVTGRKS RDG  T NTA+FAA+G  FLCK+MGF NTA
Sbjct: 301 EYITVTKDMKNVFMYGDGPRKTIVTGRKSNRDGFTTQNTASFAAIGEGFLCKSMGFTNTA 360

Query: 361 GPEGHQAVALRVQSDRSAFYECRIDGYQDSLYAQTNRQFYRNCVISGTVDFIFGESSTII 420
           GP+GHQAVALRVQSDRSAF+ CR+DG+QD+LY QT+RQFYRNCVISGTVDFIFG+SSTII
Sbjct: 361 GPQGHQAVALRVQSDRSAFFNCRMDGHQDTLYVQTHRQFYRNCVISGTVDFIFGDSSTII 420

Query: 421 QNSLIIVRRPMQGQQNTVTADGRKENRELSGLVIHNCRIVPEQKLFADRFKIPTFLGRPW 480
           QNSLIIVRRPM  QQNTVTA GR E +E++GLVIHNCRIVPEQKLFA+RFKIPTFLGRPW
Sbjct: 421 QNSLIIVRRPMDNQQNTVTAQGRAEQKEITGLVIHNCRIVPEQKLFAERFKIPTFLGRPW 480

Query: 481 KKYALTVIMESTLGDLIQPAGYMPWEED-SRDTCQYLEYANRGPGANTARRVKWKGVRVI 540
           K+YA TVIMESTLGD IQPAGYMPW  + + +TC YLEY NRGPGANT RRV+WKGV+VI
Sbjct: 481 KQYARTVIMESTLGDFIQPAGYMPWSGNFALETCLYLEYGNRGPGANTNRRVRWKGVKVI 540

Query: 541 GRNEALKFTVGSPFLIGNVWLPETRGKFLPGLKN 574
           GRNEAL++T G+ FL+G  WLP T G +  GLKN
Sbjct: 541 GRNEALQYTAGA-FLLGRTWLPRTGGIYYLGLKN 567

BLAST of Moc04g28790 vs. ExPASy TrEMBL
Match: A0A1S3CSH2 (Pectinesterase OS=Cucumis melo OX=3656 GN=LOC103503817 PE=3 SV=1)

HSP 1 Score: 799.3 bits (2063), Expect = 1.1e-227
Identity = 407/574 (70.91%), Postives = 477/574 (83.10%), Query Frame = 0

Query: 1   MVGKAAVSVLSLILVVGVALAVVAVVNMNKSGSLDTENMSPKMKAIASICSQTDYQKECQ 60
           MVGK  VS LSLILVVG+ALAVVA +N     +   E +SPKMKAI++ICS TDYQ+EC+
Sbjct: 1   MVGKVIVSALSLILVVGIALAVVATINKRNGSNGGKEEVSPKMKAISAICSTTDYQEECK 60

Query: 61  DTLGVLAKNSTSDDPKEYIKTAIMATVDEIKKGFNLTDSLMVEAGNSAFMKMSVEDCKDL 120
           +TL  +A+N++S+DPK+Y K AI+AT+ EI KG+NL+DSL+VEAGN+A +KMSVEDC DL
Sbjct: 61  NTLNHVAQNTSSNDPKDYAKAAILATIKEITKGYNLSDSLIVEAGNNASIKMSVEDCNDL 120

Query: 121 LRFAVRELQASYSSVGANDERSQEERVSDINTWLSAVISYQESCLDALGESNPKLKETMG 180
           L+FA+ ELQASYS+VG +D  +  +R++DI  WLSAV+SYQ+SCLD LGE +P+LK+ M 
Sbjct: 121 LQFAIDELQASYSTVGESDLHTDADRIADIKNWLSAVVSYQQSCLDGLGEFDPQLKQRMQ 180

Query: 181 AGSLDFATKLTSNALAIFSEVSKILGNYGLQLKAPQPNGRRLLGHTEVGSDGFPTWFSKA 240
            G LD A KLTSNALAI + VS IL NYGLQLK  QP+GRRLLG TEV SDGFPTW + A
Sbjct: 181 DG-LDVAGKLTSNALAILTAVSNILDNYGLQLKV-QPSGRRLLGTTEVDSDGFPTWLTGA 240

Query: 241 DRKLLAAARRGGGGVVGVKPNVVVAKDGSGNYKTIGAALAAAPKNSKTRYVIYVKAGIYD 300
           DRKLL A+++GG   V   PN VVAKDGSG YKTI AALAA PK  + RYVIYVKAGIYD
Sbjct: 241 DRKLL-ASKQGG---VRPTPNAVVAKDGSGQYKTIAAALAAYPKALRGRYVIYVKAGIYD 300

Query: 301 EYVTVTSDLTYIFMYGDGPRKTIVTGRKSARDGINTMNTATFAAVGFHFLCKAMGFQNTA 360
           EY+TVT D+  +FMYGDGPRKTIVTGRKS RDG  T NTA+FAA+G  FLCK+MGF NTA
Sbjct: 301 EYITVTKDMKNVFMYGDGPRKTIVTGRKSNRDGFTTQNTASFAAIGEGFLCKSMGFTNTA 360

Query: 361 GPEGHQAVALRVQSDRSAFYECRIDGYQDSLYAQTNRQFYRNCVISGTVDFIFGESSTII 420
           GP+GHQAVALRVQSDRSAF+ CR+DG+QD+LY QT+RQFYRNCVISGTVDFIFG+SSTII
Sbjct: 361 GPQGHQAVALRVQSDRSAFFNCRMDGHQDTLYVQTHRQFYRNCVISGTVDFIFGDSSTII 420

Query: 421 QNSLIIVRRPMQGQQNTVTADGRKENRELSGLVIHNCRIVPEQKLFADRFKIPTFLGRPW 480
           QNSLIIVRRPM  QQNTVTA GR E +E++GLVIHNCRIVPEQKLFA+RFKIPTFLGRPW
Sbjct: 421 QNSLIIVRRPMDNQQNTVTAQGRAEQKEITGLVIHNCRIVPEQKLFAERFKIPTFLGRPW 480

Query: 481 KKYALTVIMESTLGDLIQPAGYMPWEED-SRDTCQYLEYANRGPGANTARRVKWKGVRVI 540
           K+YA TVIMESTLGD IQPAGYMPW  + + +TC YLEY NRGPGANT RRV+WKGV+VI
Sbjct: 481 KQYARTVIMESTLGDFIQPAGYMPWSGNFALETCLYLEYGNRGPGANTNRRVRWKGVKVI 540

Query: 541 GRNEALKFTVGSPFLIGNVWLPETRGKFLPGLKN 574
           GRNEAL++T G+ FL+G  WLP T G +  GLKN
Sbjct: 541 GRNEALQYTAGA-FLLGRTWLPRTGGIYYLGLKN 567

BLAST of Moc04g28790 vs. ExPASy TrEMBL
Match: A0A0A0KLN2 (Pectinesterase OS=Cucumis sativus OX=3659 GN=Csa_5G119700 PE=3 SV=1)

HSP 1 Score: 795.4 bits (2053), Expect = 1.5e-226
Identity = 413/574 (71.95%), Postives = 475/574 (82.75%), Query Frame = 0

Query: 1   MVGKAAVSVLSLILVVGVALAVVAVVNMNKSGSLDTENMSPKMKAIASICSQTDYQKECQ 60
           MVGK  +S +SLILVVGVALAVVA+VN + S S +T N+SPKMKA+ASICS  DYQ ECQ
Sbjct: 1   MVGKVVMSGISLILVVGVALAVVAIVNKSDS-SDETANLSPKMKAVASICSNADYQPECQ 60

Query: 61  DTLGVLAKNSTSDDPKEYIKTAIMATVDEIKKGFNLTDSLMVEAGNSAFMKMSVEDCKDL 120
            TLG +  NS+ +DPKE+IK AI +T++E+KKG+NLTD+LMVEA N+A +KMSV+DCKDL
Sbjct: 61  TTLGSVGNNSSVEDPKEFIKAAITSTIEEMKKGYNLTDNLMVEAANNATIKMSVDDCKDL 120

Query: 121 LRFAVRELQASYSSVGANDERSQEERVSDINTWLSAVISYQESCLDALGESNPKLKETMG 180
           L+ A+ EL ASYS+VG  D  + E+R++DI  WL+AVISYQ+SCLD L E +P+LK+ M 
Sbjct: 121 LQSAIDELHASYSTVGDPDLHTNEDRIADIKNWLTAVISYQQSCLDGLEEFDPQLKQKMQ 180

Query: 181 AGSLDFATKLTSNALAIFSEVSKILGNYGLQLKAPQPNGRRLLGHTEVGSDGFPTWFSKA 240
            G LD A KLTSNALAI   VS IL   GLQLK  QP+GRRLLG TEV SD FPTW + +
Sbjct: 181 DG-LDVAGKLTSNALAIVGAVSDILKELGLQLKV-QPSGRRLLGTTEVDSDSFPTWLTGS 240

Query: 241 DRKLLAAARRGGGGVVGVKPNVVVAKDGSGNYKTIGAALAAAPKNSKTRYVIYVKAGIYD 300
           DRKLL AA+RGG   V VKPNVVVAKDGSG YKTIGAALAA PK  K RYVIYVKAG+Y+
Sbjct: 241 DRKLL-AAKRGG---VRVKPNVVVAKDGSGQYKTIGAALAAYPKALKGRYVIYVKAGVYN 300

Query: 301 EYVTVTSDLTYIFMYGDGPRKTIVTGRKSARDGINTMNTATFAAVGFHFLCKAMGFQNTA 360
           E + VT D+  IFMYGDGPRKTIVTGRKS RDGI T NTA+FAA+G  FLCK+MGF NTA
Sbjct: 301 EPIIVTKDMKNIFMYGDGPRKTIVTGRKSNRDGITTQNTASFAAIGEGFLCKSMGFTNTA 360

Query: 361 GPEGHQAVALRVQSDRSAFYECRIDGYQDSLYAQTNRQFYRNCVISGTVDFIFGESSTII 420
           GPEGHQAVALRVQSDRSAF+ CR+DGYQD+LY QT+RQFYRNCVISGTVDFIFG+S+TII
Sbjct: 361 GPEGHQAVALRVQSDRSAFFNCRMDGYQDTLYVQTHRQFYRNCVISGTVDFIFGDSNTII 420

Query: 421 QNSLIIVRRPMQGQQNTVTADGRKENRELSGLVIHNCRIVPEQKLFADRFKIPTFLGRPW 480
           QNSLIIVRRPM  QQNTVTA+GR + +E+SGLVIHNCRIVPEQKLFA+RFKIPTFLGRPW
Sbjct: 421 QNSLIIVRRPMDNQQNTVTANGRADKKEISGLVIHNCRIVPEQKLFAERFKIPTFLGRPW 480

Query: 481 KKYALTVIMESTLGDLIQPAGYMPWEED-SRDTCQYLEYANRGPGANTARRVKWKGVRVI 540
           K+YA TVIMESTLGD IQPAGYMPW  + +  TC Y EY NRGPGANT RRV+WKGVRVI
Sbjct: 481 KEYARTVIMESTLGDFIQPAGYMPWSGNFALATCSYFEYGNRGPGANTNRRVRWKGVRVI 540

Query: 541 GRNEALKFTVGSPFLIGNVWLPETRGKFLPGLKN 574
           GRNEA++FT G PFL+G  WLP T G +L GLKN
Sbjct: 541 GRNEAMQFTAG-PFLLGKAWLPGTGGPYLLGLKN 566

BLAST of Moc04g28790 vs. TAIR 10
Match: AT5G27870.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 433.3 bits (1113), Expect = 3.0e-121
Identity = 230/563 (40.85%), Postives = 346/563 (61.46%), Query Frame = 0

Query: 10  LSLILVVGVALAVVAVVNMNKSGSLDTENMSPKMKAIASICSQTDYQKECQDTLGVLAKN 69
           +S +L++ + +AV   V++NKS +   E ++  +KAI  +C+ TDY++ C+DTL   AK+
Sbjct: 21  ISSVLLISMVVAVTIGVSVNKSDNAGDEEITTSVKAIKDVCAPTDYKETCEDTLRKDAKD 80

Query: 70  STSDDPKEYIKTAIMATVDEIKKGFNLTDSLMVEAGNSAFMKMSVEDCKDLLRFAVRELQ 129
           ++  DP E +KTA  AT+ +I      + + M+E       KM+++ CK+L+ +A+ EL 
Sbjct: 81  TS--DPLELVKTAFNATMKQISDVAKKSQT-MIELQKDPRAKMALDQCKELMDYAIGELS 140

Query: 130 ASYSSVGANDERSQEERVSDINTWLSAVISYQESCLDALGESNPKLKETMGAGSLDFATK 189
            S+  +G  +    +E +  +  WLSA IS++++CLD    +     ET+   +L  A +
Sbjct: 141 KSFEELGKFEFHKVDEALVKLRIWLSATISHEQTCLDGFQGTQGNAGETI-KKALKTAVQ 200

Query: 190 LTSNALAIFSEVSKILGNYGLQLKAPQPNGRRLLGHTEVGSDGFPTWFSKADRKLLAAAR 249
           LT N LA+ +E+S  LG    Q++ P+ N RRLL      S  FP+W     R+LL A  
Sbjct: 201 LTHNGLAMVTEMSNYLG----QMQIPEMNSRRLL------SQEFPSWMDARARRLLNAP- 260

Query: 250 RGGGGVVGVKPNVVVAKDGSGNYKTIGAALAAAPKNSKTRYVIYVKAGIYDEYVTVTSDL 309
                +  VKP++VVA+DGSG YKTI  AL   PK   T +V+++K GIY EYV V   +
Sbjct: 261 -----MSEVKPDIVVAQDGSGQYKTINEALNFVPKKKNTTFVVHIKEGIYKEYVQVNRSM 320

Query: 310 TYIFMYGDGPRKTIVTGRKSARDGINTMNTATFAAVGFHFLCKAMGFQNTAGPEGHQAVA 369
           T++   GDGP KT+++G KS +DGI T  TAT A VG HF+ K + F+NTAG   HQAVA
Sbjct: 321 THLVFIGDGPDKTVISGSKSYKDGITTYKTATVAIVGDHFIAKNIAFENTAGAIKHQAVA 380

Query: 370 LRVQSDRSAFYECRIDGYQDSLYAQTNRQFYRNCVISGTVDFIFGESSTIIQNSLIIVRR 429
           +RV +D S FY C+ DGYQD+LYA ++RQFYR+C ISGT+DF+FG+++ + QN  ++VR+
Sbjct: 381 IRVLADESIFYNCKFDGYQDTLYAHSHRQFYRDCTISGTIDFLFGDAAAVFQNCTLLVRK 440

Query: 430 PMQGQQNTVTADGRKENRELSGLVIHNCRIVPEQKLFADRFKIPTFLGRPWKKYALTVIM 489
           P+  Q   +TA GRK+ RE +G V+  C IV E    A + +  T+LGRPWK+Y+ T+IM
Sbjct: 441 PLLNQACPITAHGRKDPRESTGFVLQGCTIVGEPDYLAVKEQSKTYLGRPWKEYSRTIIM 500

Query: 490 ESTLGDLIQPAGYMPW-EEDSRDTCQYLEYANRGPGANTARRVKWKGVRVIGRNEALKFT 549
            + + D + P G+ PW  E   +T  Y E  N GPGA   +RV W G++ +   E LKFT
Sbjct: 501 NTFIPDFVPPEGWQPWLGEFGLNTLFYSEVQNTGPGAAITKRVTWPGIKKLSDEEILKFT 560

Query: 550 VGSPFLIGNVWLPETRGKFLPGL 572
             + ++ G+ W+P     ++ GL
Sbjct: 561 -PAQYIQGDAWIPGKGVPYILGL 562

BLAST of Moc04g28790 vs. TAIR 10
Match: AT3G05610.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 427.9 bits (1099), Expect = 1.2e-119
Identity = 231/566 (40.81%), Postives = 345/566 (60.95%), Query Frame = 0

Query: 7   VSVLSLILVVGVALAVVAVVNMNK--SGSLDTENMSPKMKAIASICSQTDYQKECQDTLG 66
           V  +S +L++ + +AV   V++NK    S     ++  +KA+  +C+ TDY+K C+DTL 
Sbjct: 17  VITISSVLLISMVVAVTVGVSLNKHDGDSKGKAEVNASVKAVKDVCAPTDYRKTCEDTLI 76

Query: 67  VLAKNSTSDDPKEYIKTAIMATVDEIKKGFNLTDSLMVEAGNSAFMKMSVEDCKDLLRFA 126
              KN+T  DP E +KTA   T+ +I      + ++M E    +  +M+++ CK+L+ +A
Sbjct: 77  KNGKNTT--DPMELVKTAFNVTMKQITDAAKKSQTIM-ELQKDSRTRMALDQCKELMDYA 136

Query: 127 VRELQASYSSVGANDERSQEERVSDINTWLSAVISYQESCLDALGESNPKLKETMGAGSL 186
           + EL  S+  +G  +    +E + ++  WLSA IS++E+CL+    +     ETM   +L
Sbjct: 137 LDELSNSFEELGKFEFHLLDEALINLRIWLSAAISHEETCLEGFQGTQGNAGETM-KKAL 196

Query: 187 DFATKLTSNALAIFSEVSKILGNYGLQLKAPQPNGRRLLGHTEVGSDGFPTWFSKADRKL 246
             A +LT N LAI SE+S  +G    Q++ P  N RRLL      ++GFP+W  +  RKL
Sbjct: 197 KTAIELTHNGLAIISEMSNFVG----QMQIPGLNSRRLL------AEGFPSWVDQRGRKL 256

Query: 247 LAAARRGGGGVVGVKPNVVVAKDGSGNYKTIGAALAAAPKNSKTRYVIYVKAGIYDEYVT 306
           L AA         VKP++VVA+DGSG YKTI  AL   PK   T +V+++KAG+Y EYV 
Sbjct: 257 LQAA----AAYSDVKPDIVVAQDGSGQYKTINEALQFVPKKRNTTFVVHIKAGLYKEYVQ 316

Query: 307 VTSDLTYIFMYGDGPRKTIVTGRKSARDGINTMNTATFAAVGFHFLCKAMGFQNTAGPEG 366
           V   ++++   GDGP KTI++G K+ +DGI T  TAT A VG +F+ K +GF+NTAG   
Sbjct: 317 VNKTMSHLVFIGDGPDKTIISGNKNYKDGITTYRTATVAIVGNYFIAKNIGFENTAGAIK 376

Query: 367 HQAVALRVQSDRSAFYECRIDGYQDSLYAQTNRQFYRNCVISGTVDFIFGESSTIIQNSL 426
           HQAVA+RVQSD S F+ CR DGYQD+LY  ++RQF+R+C ISGT+DF+FG+++ + QN  
Sbjct: 377 HQAVAVRVQSDESIFFNCRFDGYQDTLYTHSHRQFFRDCTISGTIDFLFGDAAAVFQNCT 436

Query: 427 IIVRRPMQGQQNTVTADGRKENRELSGLVIHNCRIVPEQKLFADRFKIPTFLGRPWKKYA 486
           ++VR+P+  Q   +TA GRK+ RE +G V   C I  E    A +     +LGRPWK+Y+
Sbjct: 437 LLVRKPLPNQACPITAHGRKDPRESTGFVFQGCTIAGEPDYLAVKETSKAYLGRPWKEYS 496

Query: 487 LTVIMESTLGDLIQPAGYMPWEED-SRDTCQYLEYANRGPGANTARRVKWKGVRVIGRNE 546
            T+IM + + D +QP G+ PW  D    T  Y E  N GPG+  A RV W G++ +   +
Sbjct: 497 RTIIMNTFIPDFVQPQGWQPWLGDFGLKTLFYSEVQNTGPGSALANRVTWAGIKTLSEED 556

Query: 547 ALKFTVGSPFLIGNVWLPETRGKFLP 570
            LKFT  + ++ G+ W+P   GK +P
Sbjct: 557 ILKFT-PAQYIQGDDWIP---GKGVP 560

BLAST of Moc04g28790 vs. TAIR 10
Match: AT2G47030.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 414.5 bits (1064), Expect = 1.4e-115
Identity = 247/570 (43.33%), Postives = 342/570 (60.00%), Query Frame = 0

Query: 1   MVGKAAVSVLSLILVVGVALAVVAVVNMNKSGSLDTENMSPKMKAIASICSQTDYQKECQ 60
           M+GK  VSV S++L+VGVA+ VVA +N N        N+SP+MKA+  IC  T  +  C 
Sbjct: 1   MIGKVVVSVASILLIVGVAIGVVAFINKNGDA-----NLSPQMKAVQGICQSTSDKASCV 60

Query: 61  DTLGVLAKNSTSDDPKEYIKTAIMATVDEIKKGFNLTDSLMVEAGNSAF--MKMSVEDCK 120
            TL    +   S+DP + IK  ++AT DE+ K  N T    V  G+S     K  ++ CK
Sbjct: 61  KTL----EPVKSEDPNKLIKAFMLATKDELTKSSNFTGQTEVNMGSSISPNNKAVLDYCK 120

Query: 121 DLLRFAVRELQASYSSVGANDERSQEERVSDINTWLSAVISYQESCLDALGESNPKLKET 180
            +  +A+ +L      +G  D      ++  +  WL  V +YQ  CLD + E +  L++ 
Sbjct: 121 RVFMYALEDLATIIEEMG-EDLSQIGSKIDQLKQWLIGVYNYQTDCLDDIEEDD--LRKA 180

Query: 181 MGAGSLDFATKLTSNALAIF----SEVSKI------LGNYGLQLKAP------------Q 240
           +G G  + +  LT+NA+ IF    S ++KI      L N    +  P             
Sbjct: 181 IGEGIAN-SKILTTNAIDIFHTVVSAMAKINNKVDDLKNMTGGIPTPGAPPVVDESPVAD 240

Query: 241 PNG--RRLLGHTEVGSDGFPTWFSKADRKLLAAA---RRGG-GGVVGVKPNVVVAKDGSG 300
           P+G  RRLL   ++   G PTW S ADRKL+A A   RRGG GG   V+ N VVAKDGSG
Sbjct: 241 PDGPARRLL--EDIDETGIPTWVSGADRKLMAKAGRGRRGGRGGGARVRTNFVVAKDGSG 300

Query: 301 NYKTIGAALAAAPKNSKTRYVIYVKAGIYDEYVTVTSDLTYIFMYGDGPRKTIVTGRKSA 360
            +KT+  A+ A P+N++ R +IY+KAG+Y E V +      IFM+GDG RKT+++  +S 
Sbjct: 301 QFKTVQQAVDACPENNRGRCIIYIKAGLYREQVIIPKKKNNIFMFGDGARKTVISYNRSV 360

Query: 361 --RDGINTMNTATFAAVGFHFLCKAMGFQNTAGPEGHQAVALRVQSDRSAFYECRIDGYQ 420
               G  T  +AT       F+ K MGF+NTAGP GHQA A+RV  DR+  + CR DGYQ
Sbjct: 361 ALSRGTTTSLSATVQVESEGFMAKWMGFKNTAGPMGHQAAAIRVNGDRAVIFNCRFDGYQ 420

Query: 421 DSLYAQTNRQFYRNCVISGTVDFIFGESSTIIQNSLIIVRRPMQGQQNTVTADGRKENRE 480
           D+LY    RQFYRNCV+SGTVDFIFG+S+T+IQN+LI+VR+  +GQ NTVTADG +    
Sbjct: 421 DTLYVNNGRQFYRNCVVSGTVDFIFGKSATVIQNTLIVVRKGSKGQYNTVTADGNELGLG 480

Query: 481 LS-GLVIHNCRIVPEQKLFADRFKIPTFLGRPWKKYALTVIMESTLGDLIQPAGYMPWEE 537
           +  G+V+ NCRIVP++KL  +R  + T+LGRPWKK++ TVIM + +GDLI+P G+  W+ 
Sbjct: 481 MKIGIVLQNCRIVPDRKLTPERLTVATYLGRPWKKFSTTVIMSTEMGDLIRPEGWKIWDG 540

BLAST of Moc04g28790 vs. TAIR 10
Match: AT4G33230.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 411.8 bits (1057), Expect = 9.2e-115
Identity = 238/553 (43.04%), Postives = 330/553 (59.67%), Query Frame = 0

Query: 27  NMNKSGSLDTENMSPKM--------------KAIASICSQTDYQKECQDTL-GVLAKNST 86
           N +K     +E+ SPK               K I ++C+ T Y+  CQ+TL     K++ 
Sbjct: 62  NKSKDSPTKSESPSPKPPSSAAQTVKAGQVDKIIQTLCNSTLYKPTCQNTLKNETKKDTP 121

Query: 87  SDDPKEYIKTAIMATVDEIKKGFNLTDSLMVEAGNSAFMKMSVEDCKDLLRFAVRELQAS 146
             DP+  +K+AI+A  D++ + F    SL  E  +    K ++  CK L+  A  EL  S
Sbjct: 122 QTDPRSLLKSAIVAVNDDLDQVFKRVLSLKTENKDD---KDAIAQCKLLVDEAKEELGTS 181

Query: 147 YSSVGANDERSQEERVSDINTWLSAVISYQESCLDALGESNPKLKETMGAGSLDFATKLT 206
              +  ++  +  + V D+++WLSAV+SYQE+C+D   E   KLK T    + + +  LT
Sbjct: 182 MKRINDSEVNNFAKIVPDLDSWLSAVMSYQETCVDGFEEG--KLK-TEIRKNFNSSQVLT 241

Query: 207 SNALAIFSEVSKILGNYGLQLKAPQPNGRRLL--GHTEVGSDGFPTWFSKADRKLLAAAR 266
           SN+LA+   +   L +       P+   R LL    +   +D   +W S  +R++L A  
Sbjct: 242 SNSLAMIKSLDGYLSS------VPKVKTRLLLEARSSAKETDHITSWLSNKERRMLKAV- 301

Query: 267 RGGGGVVGVKPNVVVAKDGSGNYKTIGAALAAAPKNSKTRYVIYVKAGIYDEYVTVTSDL 326
                V  +KPN  VAKDGSGN+ TI AAL A P   + RY IY+K GIYDE V +    
Sbjct: 302 ----DVKALKPNATVAKDGSGNFTTINAALKAMPAKYQGRYTIYIKHGIYDESVIIDKKK 361

Query: 327 TYIFMYGDGPRKTIVTGRKSARDGINTMNTATFAAVGFHFLCKAMGFQNTAGPEGHQAVA 386
             + M GDG +KTIVTG KS    I T  TATF A G  F+ ++MGF+NTAGPEGHQAVA
Sbjct: 362 PNVTMVGDGSQKTIVTGNKSHAKKIRTFLTATFVAQGEGFMAQSMGFRNTAGPEGHQAVA 421

Query: 387 LRVQSDRSAFYECRIDGYQDSLYAQTNRQFYRNCVISGTVDFIFGESSTIIQNSLIIVRR 446
           +RVQSDRS F  CR +GYQD+LYA T+RQ+YR+CVI GTVDFIFG+++ I QN  I +R+
Sbjct: 422 IRVQSDRSVFLNCRFEGYQDTLYAYTHRQYYRSCVIIGTVDFIFGDAAAIFQNCDIFIRK 481

Query: 447 PMQGQQNTVTADGRKENRELSGLVIHNCRIVPEQKLFADRFKIPTFLGRPWKKYALTVIM 506
            + GQ+NTVTA GR +  + +G VIHNC + P + L   + +  ++LGRPWK ++ TV+M
Sbjct: 482 GLPGQKNTVTAQGRVDKFQTTGFVIHNCTVAPNEDLKPVKAQFKSYLGRPWKPHSRTVVM 541

Query: 507 ESTLGDLIQPAGYMPWEED--SRDTCQYLEYANRGPGANTARRVKWKGVRVIGRNEALKF 561
           EST+ D+I P G++ W+E   + DT  Y EY N GP   TA RVKW G RV+ + EA+KF
Sbjct: 542 ESTIEDVIDPVGWLRWQETDFAIDTLSYAEYKNDGPSGATAARVKWPGFRVLNKEEAMKF 595

BLAST of Moc04g28790 vs. TAIR 10
Match: AT2G26450.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 409.8 bits (1052), Expect = 3.5e-114
Identity = 233/524 (44.47%), Postives = 321/524 (61.26%), Query Frame = 0

Query: 44  KAIASICSQTDYQKECQDTLGVLAKNSTS-----DDPKEYIKTAIMATVDEIKKGFNLTD 103
           K I ++CS T Y + C+ TL    KN T      D+P  ++K+AI A  +++        
Sbjct: 107 KIIQTLCSSTLYMQICEKTL----KNRTDKGFALDNPTTFLKSAIEAVNEDLDLVLEKVL 166

Query: 104 SLMVEAGNSAFMKMSVEDCKDLLRFAVRELQASYSSVGANDERSQEERVSDINTWLSAVI 163
           SL  E  +    K ++E CK L+  A  E  AS + +   +  S E+ V D+ +WLSAV+
Sbjct: 167 SLKTENQDD---KDAIEQCKLLVEDAKEETVASLNKINVTEVNSFEKVVPDLESWLSAVM 226

Query: 164 SYQESCLDALGESNPKLKETMGAGSLDFATKLTSNALAIFSEVSKILGNYGLQLKAPQPN 223
           SYQE+CLD   E N K +      S++ +  LTSN+LA+    ++   N    +K  +  
Sbjct: 227 SYQETCLDGFEEGNLKSEVKT---SVNSSQVLTSNSLALIKTFTE---NLSPVMKVVE-- 286

Query: 224 GRRLLGHTEVGSDGFPTWFSKADRKLLAAARRGGGGVVGVKPNVVVAKDGSGNYKTIGAA 283
            R LL       D  P+W S  DR++L A       V  +KPN  VAKDGSG++ TI  A
Sbjct: 287 -RHLL-------DDIPSWVSNDDRRMLRAV-----DVKALKPNATVAKDGSGDFTTINDA 346

Query: 284 LAAAPKNSKTRYVIYVKAGIYDEYVTVTSDLTYIFMYGDGPRKTIVTGRKSARDGINTMN 343
           L A P+  + RY+IYVK GIYDEYVTV      + M GDG +KTIVTG KS    I T  
Sbjct: 347 LRAMPEKYEGRYIIYVKQGIYDEYVTVDKKKANLTMVGDGSQKTIVTGNKSHAKKIRTFL 406

Query: 344 TATFAAVGFHFLCKAMGFQNTAGPEGHQAVALRVQSDRSAFYECRIDGYQDSLYAQTNRQ 403
           TATF A G  F+ ++MGF+NTAGPEGHQAVA+RVQSDRS F  CR +GYQD+LYA T+RQ
Sbjct: 407 TATFVAQGEGFMAQSMGFRNTAGPEGHQAVAIRVQSDRSIFLNCRFEGYQDTLYAYTHRQ 466

Query: 404 FYRNCVISGTVDFIFGESSTIIQNSLIIVRRPMQGQQNTVTADGRKENRELSGLVIHNCR 463
           +YR+CVI GT+DFIFG+++ I QN  I +R+ + GQ+NTVTA GR +  + +G V+HNC+
Sbjct: 467 YYRSCVIVGTIDFIFGDAAAIFQNCNIFIRKGLPGQKNTVTAQGRVDKFQTTGFVVHNCK 526

Query: 464 IVPEQKLFADRFKIPTFLGRPWKKYALTVIMESTLGDLIQPAGYMPWEED--SRDTCQYL 523
           I   + L   + +  ++LGRPWK Y+ T+IMES + ++I P G++ W+E   + DT  Y 
Sbjct: 527 IAANEDLKPVKEEYKSYLGRPWKNYSRTIIMESKIENVIDPVGWLRWQETDFAIDTLYYA 586

Query: 524 EYANRGPGANTARRVKWKGVRVIGRNEALKFTVGSPFLIGNVWL 561
           EY N+G   +T  RVKW G +VI + EAL +TVG PFL G+ W+
Sbjct: 587 EYNNKGSSGDTTSRVKWPGFKVINKEEALNYTVG-PFLQGD-WI 600

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022146322.10.0e+00100.00pectinesterase-like [Momordica charantia][more]
XP_022146333.14.3e-26381.68pectinesterase-like [Momordica charantia][more]
KAA0050274.11.3e-22771.08pectinesterase-like [Cucumis melo var. makuwa] >TYJ98133.1 pectinesterase-like [... [more]
XP_008466403.12.2e-22770.91PREDICTED: pectinesterase-like [Cucumis melo][more]
KGN49779.13.2e-22671.95hypothetical protein Csa_017783 [Cucumis sativus][more]
Match NameE-valueIdentityDescription
Q3E8Z84.2e-12040.85Putative pectinesterase/pectinesterase inhibitor 28 OS=Arabidopsis thaliana OX=3... [more]
Q8GX861.8e-11840.81Probable pectinesterase/pectinesterase inhibitor 21 OS=Arabidopsis thaliana OX=3... [more]
O807222.0e-11443.33Pectinesterase 4 OS=Arabidopsis thaliana OX=3702 GN=PME4 PE=2 SV=1[more]
Q9SMY61.3e-11343.04Putative pectinesterase/pectinesterase inhibitor 45 OS=Arabidopsis thaliana OX=3... [more]
Q7Y2014.9e-11344.47Probable pectinesterase/pectinesterase inhibitor 13 OS=Arabidopsis thaliana OX=3... [more]
Match NameE-valueIdentityDescription
A0A6J1CZA20.0e+00100.00Pectinesterase OS=Momordica charantia OX=3673 GN=LOC111015559 PE=3 SV=1[more]
A0A6J1CYB22.1e-26381.68Pectinesterase OS=Momordica charantia OX=3673 GN=LOC111015570 PE=3 SV=1[more]
A0A5A7U4V76.3e-22871.08Pectinesterase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold180G00110... [more]
A0A1S3CSH21.1e-22770.91Pectinesterase OS=Cucumis melo OX=3656 GN=LOC103503817 PE=3 SV=1[more]
A0A0A0KLN21.5e-22671.95Pectinesterase OS=Cucumis sativus OX=3659 GN=Csa_5G119700 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT5G27870.13.0e-12140.85Plant invertase/pectin methylesterase inhibitor superfamily [more]
AT3G05610.11.2e-11940.81Plant invertase/pectin methylesterase inhibitor superfamily [more]
AT2G47030.11.4e-11543.33Plant invertase/pectin methylesterase inhibitor superfamily [more]
AT4G33230.19.2e-11543.04Plant invertase/pectin methylesterase inhibitor superfamily [more]
AT2G26450.13.5e-11444.47Plant invertase/pectin methylesterase inhibitor superfamily [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (OHB3-1) v2
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR006501Pectinesterase inhibitor domainSMARTSM00856PMEI_2coord: 41..197
e-value: 1.9E-33
score: 127.1
IPR006501Pectinesterase inhibitor domainPFAMPF04043PMEIcoord: 45..197
e-value: 6.4E-24
score: 85.0
IPR006501Pectinesterase inhibitor domainTIGRFAMTIGR01614TIGR01614coord: 24..200
e-value: 2.2E-19
score: 67.4
IPR012334Pectin lyase foldGENE3D2.160.20.10coord: 255..571
e-value: 2.7E-104
score: 351.0
IPR035513Invertase/pectin methylesterase inhibitor domain superfamilyGENE3D1.20.140.40Invertase/pectin methylesterase inhibitor family proteincoord: 42..202
e-value: 1.0E-27
score: 99.0
IPR035513Invertase/pectin methylesterase inhibitor domain superfamilySUPERFAMILY101148Plant invertase/pectin methylesterase inhibitorcoord: 44..201
IPR000070Pectinesterase, catalyticPFAMPF01095Pectinesterasecoord: 261..551
e-value: 2.8E-124
score: 413.7
NoneNo IPR availablePANTHERPTHR31707PECTINESTERASEcoord: 1..571
NoneNo IPR availablePANTHERPTHR31707:SF292PECTINESTERASEcoord: 1..571
NoneNo IPR availableCDDcd15798PMEI-like_3coord: 48..203
e-value: 1.12483E-47
score: 161.837
IPR018040Pectinesterase, Tyr active sitePROSITEPS00800PECTINESTERASE_1coord: 286..305
IPR033131Pectinesterase, Asp active sitePROSITEPS00503PECTINESTERASE_2coord: 405..414
IPR011050Pectin lyase fold/virulence factorSUPERFAMILY51126Pectin lyase-likecoord: 258..568

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Moc04g28790.1Moc04g28790.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0042545 cell wall modification
biological_process GO:0043086 negative regulation of catalytic activity
biological_process GO:0045490 pectin catabolic process
cellular_component GO:0005576 extracellular region
molecular_function GO:0045330 aspartyl esterase activity
molecular_function GO:0030599 pectinesterase activity
molecular_function GO:0046910 pectinesterase inhibitor activity
molecular_function GO:0004857 enzyme inhibitor activity