Moc04g28330 (gene) Bitter gourd (OHB3-1) v2

Overview
NameMoc04g28330
Typegene
OrganismMomordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
DescriptionRibonuclease H
Locationchr4: 20952601 .. 20953464 (+)
RNA-Seq ExpressionMoc04g28330
SyntenyMoc04g28330
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCTCCGTACCACAACGCTAGAGTCATGCCACGGTCGTTCAAGCTAGGGAACCTTGTCCTCAAAAAGGTTCTGGCCCATGTGAGGGCTCTGGAGCCAAACTGGTCAAGCCCGTTCAAGGTTCGCGGGGTAGTTCAGTCCGGCACACACATCATCACCGACCTGAACGACAACAAACTACCTCACCTATGGAATGCTCAGCATCTATGTGTTTATTACCAATAAATGTAAGACAATCTAAGTGTAACTAGGTTGTATGCAATATTAATAAACACCGATCTGTTCTTATCTAAGTTTACCTCGTCTGCCTGAGCTAAACTTAAATGGGGCCGAGCTATGATATCAGATTTGAAAGTGAGACCAATTCACCGATCGGCTCGGATCATACTCCCCCCATTAACTAGTTGCGTTATTATTATAAAAAAAAGATTTTTTTTATATATACACATTAAGCAATCAAGCAAGTCAGTTTGGCAAATAGTGGAGTTGAGTAGAAACAAAATTTTTTTTCATTCACTTACAAAGTGTGGCTGAGGCATGTAGAGCTCAACCATTACAAAACTGAGCCGAAGCAATATCGCTCCGACTCAACGAGACTTTGCAAAGAAAAAAAGAGAGAAGAGATGATAATAGCTATCAGGTGAGGTGCGTCACGCTCCGACGTTCGGATCGTTCTGTCCATCCATCTGACTCGCAACTCGAACCTCCTCATCGTCCTCGACCTCCAGCTCGGCCTCATAATCCTGATACACTAAAGTGATGTCCAACTCAGGTGCCAGCTTTCTCACGGCATCAGTGGCGAATGCCTTCACCACCTCATTAAACTATGACATAACCTTTATTGTGTTTTCTAAGTCGGCGTAA

mRNA sequence

ATGGCTCCGTACCACAACGCTAGAGTCATGCCACGGTCGTTCAAGCTAGGGAACCTTGTCCTCAAAAAGGTTCTGGCCCATGTGAGGGCTCTGGAGCCAAACTGGTCAAGCCCGTTCAAGGTTCGCGGGGTAGTTCAGTCCGGCACACACATCATCACCGACCTGAACGACAACAAACTACCTCACCTATGGAATGCTCAGCATCTATGTGCCAGCTTTCTCACGGCATCAGTGGCGAATGCCTTCACCACCTCATTAAACTATGACATAACCTTTATTGTGTTTTCTAAGTCGGCGTAA

Coding sequence (CDS)

ATGGCTCCGTACCACAACGCTAGAGTCATGCCACGGTCGTTCAAGCTAGGGAACCTTGTCCTCAAAAAGGTTCTGGCCCATGTGAGGGCTCTGGAGCCAAACTGGTCAAGCCCGTTCAAGGTTCGCGGGGTAGTTCAGTCCGGCACACACATCATCACCGACCTGAACGACAACAAACTACCTCACCTATGGAATGCTCAGCATCTATGTGCCAGCTTTCTCACGGCATCAGTGGCGAATGCCTTCACCACCTCATTAAACTATGACATAACCTTTATTGTGTTTTCTAAGTCGGCGTAA

Protein sequence

MAPYHNARVMPRSFKLGNLVLKKVLAHVRALEPNWSSPFKVRGVVQSGTHIITDLNDNKLPHLWNAQHLCASFLTASVANAFTTSLNYDITFIVFSKSA
Homology
BLAST of Moc04g28330 vs. NCBI nr
Match: XP_022156986.1 (uncharacterized protein LOC111023816 [Momordica charantia])

HSP 1 Score: 88.2 bits (217), Expect = 4.3e-14
Identity = 37/69 (53.62%), Postives = 52/69 (75.36%), Query Frame = 0

Query: 1   MAPYHNARVMPRSFKLGNLVLKKVLAHVRALEPNWSSPFKVRGVVQSGTHIITDLNDNKL 60
           MA Y+NARV  R+FK  +LVLKKV+ HV ALEPNW  P++V  +++   ++++DLN  +L
Sbjct: 772 MARYYNARVKVRTFKPSDLVLKKVMQHVGALEPNWEGPYRVLKILRPRAYLLSDLNGRQL 831

Query: 61  PHLWNAQHL 70
           PH WNA+HL
Sbjct: 832 PHPWNAEHL 840

BLAST of Moc04g28330 vs. NCBI nr
Match: XP_022156271.1 (uncharacterized protein LOC111023203 [Momordica charantia])

HSP 1 Score: 83.2 bits (204), Expect = 1.4e-12
Identity = 36/69 (52.17%), Postives = 49/69 (71.01%), Query Frame = 0

Query: 1   MAPYHNARVMPRSFKLGNLVLKKVLAHVRALEPNWSSPFKVRGVVQSGTHIITDLNDNKL 60
           MA Y+NARV  R FK G+LVLKKV+ HV AL+PNW  P++V  +++   ++   LN  +L
Sbjct: 182 MAWYYNARVKVRMFKPGDLVLKKVMQHVEALQPNWEGPYRVLEILRPRAYLSFHLNGRQL 241

Query: 61  PHLWNAQHL 70
           PH WNA+HL
Sbjct: 242 PHPWNAEHL 250

BLAST of Moc04g28330 vs. NCBI nr
Match: XP_022156049.1 (uncharacterized protein LOC111023017 [Momordica charantia])

HSP 1 Score: 82.4 bits (202), Expect = 2.4e-12
Identity = 36/69 (52.17%), Postives = 49/69 (71.01%), Query Frame = 0

Query: 1   MAPYHNARVMPRSFKLGNLVLKKVLAHVRALEPNWSSPFKVRGVVQSGTHIITDLNDNKL 60
           MA Y+N R   R+FK G+L LKKV+ HV ALEPNW  P++V  VV+   ++++DLN  +L
Sbjct: 118 MARYYNTRERVRTFKPGDLNLKKVMQHVEALEPNWEGPYRVLKVVRPRAYLLSDLNGRQL 177

Query: 61  PHLWNAQHL 70
           PH WNA+ L
Sbjct: 178 PHPWNAEPL 186

BLAST of Moc04g28330 vs. NCBI nr
Match: XP_022159003.1 (uncharacterized protein LOC111025448 [Momordica charantia])

HSP 1 Score: 82.0 bits (201), Expect = 3.1e-12
Identity = 32/69 (46.38%), Postives = 51/69 (73.91%), Query Frame = 0

Query: 1   MAPYHNARVMPRSFKLGNLVLKKVLAHVRALEPNWSSPFKVRGVVQSGTHIITDLNDNKL 60
           MA ++N R+ PRSF++G+LV  K+ +HV  L+P+W   F+V+G+V+ GT+++ DL  + L
Sbjct: 556 MARHYNTRIQPRSFQVGHLVSSKIQSHVGTLDPSWEELFEVKGIVRPGTYMLADLERSVL 615

Query: 61  PHLWNAQHL 70
            H WNA+HL
Sbjct: 616 AHPWNAEHL 624

BLAST of Moc04g28330 vs. NCBI nr
Match: GFZ01910.1 (hypothetical protein Acr_15g0005190 [Actinidia rufa])

HSP 1 Score: 75.5 bits (184), Expect = 2.9e-10
Identity = 34/74 (45.95%), Postives = 49/74 (66.22%), Query Frame = 0

Query: 1    MAPYHNARVMPRSFKLGNLVLKKVLAHVR-----ALEPNWSSPFKVRGVVQSGTHIITDL 60
            M  YH+ RV PR+F++G+LVLKKV    R      L PNW  P++VRG  + GT+ +T L
Sbjct: 1128 MRQYHDTRVKPRTFEMGDLVLKKVNQSTRKPSDGKLGPNWEGPYRVRGFSRKGTYWLTGL 1187

Query: 61   NDNKLPHLWNAQHL 70
            ++  LP+ W+A+HL
Sbjct: 1188 DNKDLPNPWHAEHL 1201

BLAST of Moc04g28330 vs. ExPASy TrEMBL
Match: A0A6J1DWM2 (Ribonuclease H OS=Momordica charantia OX=3673 GN=LOC111023816 PE=4 SV=1)

HSP 1 Score: 88.2 bits (217), Expect = 2.1e-14
Identity = 37/69 (53.62%), Postives = 52/69 (75.36%), Query Frame = 0

Query: 1   MAPYHNARVMPRSFKLGNLVLKKVLAHVRALEPNWSSPFKVRGVVQSGTHIITDLNDNKL 60
           MA Y+NARV  R+FK  +LVLKKV+ HV ALEPNW  P++V  +++   ++++DLN  +L
Sbjct: 772 MARYYNARVKVRTFKPSDLVLKKVMQHVGALEPNWEGPYRVLKILRPRAYLLSDLNGRQL 831

Query: 61  PHLWNAQHL 70
           PH WNA+HL
Sbjct: 832 PHPWNAEHL 840

BLAST of Moc04g28330 vs. ExPASy TrEMBL
Match: A0A6J1DUF4 (uncharacterized protein LOC111023203 OS=Momordica charantia OX=3673 GN=LOC111023203 PE=4 SV=1)

HSP 1 Score: 83.2 bits (204), Expect = 6.8e-13
Identity = 36/69 (52.17%), Postives = 49/69 (71.01%), Query Frame = 0

Query: 1   MAPYHNARVMPRSFKLGNLVLKKVLAHVRALEPNWSSPFKVRGVVQSGTHIITDLNDNKL 60
           MA Y+NARV  R FK G+LVLKKV+ HV AL+PNW  P++V  +++   ++   LN  +L
Sbjct: 182 MAWYYNARVKVRMFKPGDLVLKKVMQHVEALQPNWEGPYRVLEILRPRAYLSFHLNGRQL 241

Query: 61  PHLWNAQHL 70
           PH WNA+HL
Sbjct: 242 PHPWNAEHL 250

BLAST of Moc04g28330 vs. ExPASy TrEMBL
Match: A0A6J1DS38 (uncharacterized protein LOC111023017 OS=Momordica charantia OX=3673 GN=LOC111023017 PE=4 SV=1)

HSP 1 Score: 82.4 bits (202), Expect = 1.2e-12
Identity = 36/69 (52.17%), Postives = 49/69 (71.01%), Query Frame = 0

Query: 1   MAPYHNARVMPRSFKLGNLVLKKVLAHVRALEPNWSSPFKVRGVVQSGTHIITDLNDNKL 60
           MA Y+N R   R+FK G+L LKKV+ HV ALEPNW  P++V  VV+   ++++DLN  +L
Sbjct: 118 MARYYNTRERVRTFKPGDLNLKKVMQHVEALEPNWEGPYRVLKVVRPRAYLLSDLNGRQL 177

Query: 61  PHLWNAQHL 70
           PH WNA+ L
Sbjct: 178 PHPWNAEPL 186

BLAST of Moc04g28330 vs. ExPASy TrEMBL
Match: A0A6J1DXL9 (uncharacterized protein LOC111025448 OS=Momordica charantia OX=3673 GN=LOC111025448 PE=4 SV=1)

HSP 1 Score: 82.0 bits (201), Expect = 1.5e-12
Identity = 32/69 (46.38%), Postives = 51/69 (73.91%), Query Frame = 0

Query: 1   MAPYHNARVMPRSFKLGNLVLKKVLAHVRALEPNWSSPFKVRGVVQSGTHIITDLNDNKL 60
           MA ++N R+ PRSF++G+LV  K+ +HV  L+P+W   F+V+G+V+ GT+++ DL  + L
Sbjct: 556 MARHYNTRIQPRSFQVGHLVSSKIQSHVGTLDPSWEELFEVKGIVRPGTYMLADLERSVL 615

Query: 61  PHLWNAQHL 70
            H WNA+HL
Sbjct: 616 AHPWNAEHL 624

BLAST of Moc04g28330 vs. ExPASy TrEMBL
Match: A0A7J0FUQ7 (Ribonuclease H OS=Actinidia rufa OX=165716 GN=Acr_15g0005190 PE=4 SV=1)

HSP 1 Score: 75.5 bits (184), Expect = 1.4e-10
Identity = 34/74 (45.95%), Postives = 49/74 (66.22%), Query Frame = 0

Query: 1    MAPYHNARVMPRSFKLGNLVLKKVLAHVR-----ALEPNWSSPFKVRGVVQSGTHIITDL 60
            M  YH+ RV PR+F++G+LVLKKV    R      L PNW  P++VRG  + GT+ +T L
Sbjct: 1128 MRQYHDTRVKPRTFEMGDLVLKKVNQSTRKPSDGKLGPNWEGPYRVRGFSRKGTYWLTGL 1187

Query: 61   NDNKLPHLWNAQHL 70
            ++  LP+ W+A+HL
Sbjct: 1188 DNKDLPNPWHAEHL 1201

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022156986.14.3e-1453.62uncharacterized protein LOC111023816 [Momordica charantia][more]
XP_022156271.11.4e-1252.17uncharacterized protein LOC111023203 [Momordica charantia][more]
XP_022156049.12.4e-1252.17uncharacterized protein LOC111023017 [Momordica charantia][more]
XP_022159003.13.1e-1246.38uncharacterized protein LOC111025448 [Momordica charantia][more]
GFZ01910.12.9e-1045.95hypothetical protein Acr_15g0005190 [Actinidia rufa][more]
Match NameE-valueIdentityDescription
Match NameE-valueIdentityDescription
A0A6J1DWM22.1e-1453.62Ribonuclease H OS=Momordica charantia OX=3673 GN=LOC111023816 PE=4 SV=1[more]
A0A6J1DUF46.8e-1352.17uncharacterized protein LOC111023203 OS=Momordica charantia OX=3673 GN=LOC111023... [more]
A0A6J1DS381.2e-1252.17uncharacterized protein LOC111023017 OS=Momordica charantia OX=3673 GN=LOC111023... [more]
A0A6J1DXL91.5e-1246.38uncharacterized protein LOC111025448 OS=Momordica charantia OX=3673 GN=LOC111025... [more]
A0A7J0FUQ71.4e-1045.95Ribonuclease H OS=Actinidia rufa OX=165716 GN=Acr_15g0005190 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Moc04g28330.1Moc04g28330.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006259 DNA metabolic process
cellular_component GO:0110165 cellular anatomical entity
molecular_function GO:0140640 catalytic activity, acting on a nucleic acid
molecular_function GO:0003676 nucleic acid binding