Moc04g27770 (gene) Bitter gourd (OHB3-1) v2

Overview
NameMoc04g27770
Typegene
OrganismMomordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
DescriptionReverse transcriptase domain-containing protein
Locationchr4: 20498877 .. 20500208 (+)
RNA-Seq ExpressionMoc04g27770
SyntenyMoc04g27770
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGCACAAAAGACTCCAAAAATGGAGATCTTTGCTTCTTTCCAAGGGTGGTAGGCTTACTCTTGCTCAGTCGGTCCTCAATAGCATCCCCATATACTTCTTTTCAATTCTCAAAGCTCCAGAGACCATCATAAAGTCTATGGAGAAGCTTATGCGGGACTTCATATGGAATGGTGGGATTTCTAGATCGGTGGGGAATTTGGTGAAGTGGTCTTGGTCGGCTTTGCCTCATACACAGGGGGGCCTTGGAATTGGTTCTTTGAGGCTAAATAACAATGCTCTATTAATGAAATGGTTATGGCAGTTCACAGTGGAAAGAGATAGCCTTTGGAGACGGGTGATAGCCACTATTTACGTTGTGGAGTCTTTTGGATGGTGCACAAAACCATCGGATGGTTCTTCTATTGGAAGACCGTGGAGGGACATACAAGAGAACCTTAGAGATTTTCAAAAGTTCTCCTTCTTCAAAGTATCCTCAGGTGTCCAAGTCAGATTTTGGGAGGACCCATGAATTGATTTTGCCCCTCTTTACACCACTTTCCCGGATTTGTTTGCGTTATCCTCCAAGAAAGGTATGTCCATTGCGGATTGTTGGTGTGCAGAGGCCTCAACTTGGAATCTTCGTTTCAGAAGAAATCTCTTTGAAAGGGAAATGAGTAGATGGGCAAACTTCACTGAAAAAATAGAAGGTGCAGCTATCGGAAATGGAAGGGACATTTTATGGTGGAAAGCAGACCCATCCGGACGTTTCACAGTCAATTCAGCCTTCATGGCTCTCACGTCCCCGTCTCCTAAACTTAATTCAGCTACAGCAAACTTGATATGGAATTTTAAAGTCCCAAAGAAGGTAAAATTCTTCATTTGGACTTTGTTCTATAGAGGGGTAAATACTGCAGAGAAACTTCAGAGGAAATTACCTCACTGGACTCTTTCTCCCTCTATGTACACATAGTGTATGAGCAACAATGAAACAGTGGACCACCTTTTTCTCCATTGCCCTTTTGTTTCCAAAGCTTGGGGGTTTATAGCTTCTTTGCTAGATTTATCTCTCTGCATACCTAGAAGAATTGAGGATTTCATAGCCGAAGGTTATGGAGGAAGGCAGCTCAAGGCCAAGGCTTGGGTTCTTTGGAATTGTGCGGCCCGGGCTCTTTCATGGTCGATATGGAAGGAACGAAATAGTCAACTTTTTGAAGATAAGTTGATTTTGTTCGATTCTTTTTGTGTAAATACTAAGCATCTAGCTTCTTGGTGGGCTTTGCTATACAAGAAAGTTTTTTGTAACTACAATCTTCCTTTGCTTAGACAAGATTGGAAAGTGGTGATTGTATAG

mRNA sequence

ATGCACAAAAGACTCCAAAAATGGAGATCTTTGCTTCTTTCCAAGGGTGGTAGGCTTACTCTTGCTCAGTCGGTCCTCAATAGCATCCCCATATACTTCTTTTCAATTCTCAAAGCTCCAGAGACCATCATAAAGTCTATGGAGAAGCTTATGCGGGACTTCATATGGAATGGTGGGATTTCTAGATCGGTGGGGAATTTGGTGAAGTGGTCTTGGTCGGCTTTGCCTCATACACAGGGGGGCCTTGGAATTGGTTCTTTGAGGCTAAATAACAATGCTCTATTAATGAAATGGTTATGGCAGTTCACAGTGGAAAGAGATAGCCTTTGGAGACGGGTGATAGCCACTATTTACGTTGTGGAGTCTTTTGGATGGTGCACAAAACCATCGGATGGTTCTTCTATTGGAAGACCGTGGAGGGACATACAAGAGAACCTTAGAGATTTTCAAAAGTTCTCCTTCTTCAAAGTATCCTCAGGTATGTCCATTGCGGATTGTTGGTGTGCAGAGGCCTCAACTTGGAATCTTCGTTTCAGAAGAAATCTCTTTGAAAGGGAAATGAGTAGATGGGCAAACTTCACTGAAAAAATAGAAGGTGCAGCTATCGGAAATGGAAGGGACATTTTATGGTGGAAAGCAGACCCATCCGGACGTTTCACAGTCAATTCAGCCTTCATGGCTCTCACGTCCCCGTCTCCTAAACTTAATTCAGCTACAGCAAACTTGATATGGAATTTTAAAGTCCCAAAGAAGTGTATGAGCAACAATGAAACAGTGGACCACCTTTTTCTCCATTGCCCTTTTGTTTCCAAAGCTTGGGGGTTTATAGCTTCTTTGCTAGATTTATCTCTCTGCATACCTAGAAGAATTGAGGATTTCATAGCCGAAGGTTATGGAGGAAGGCAGCTCAAGGCCAAGGCTTGGGTTCTTTGGAATTGTGCGGCCCGGGCTCTTTCATGGTCGATATGGAAGGAACGAAATAGTCAACTTTTTGAAGATAAGTTGATTTTGTTCGATTCTTTTTGTGTAAATACTAAGCATCTAGCTTCTTGGTGGGCTTTGCTATACAAGAAAGTTTTTTGTAACTACAATCTTCCTTTGCTTAGACAAGATTGGAAAGTGGTGATTGTATAG

Coding sequence (CDS)

ATGCACAAAAGACTCCAAAAATGGAGATCTTTGCTTCTTTCCAAGGGTGGTAGGCTTACTCTTGCTCAGTCGGTCCTCAATAGCATCCCCATATACTTCTTTTCAATTCTCAAAGCTCCAGAGACCATCATAAAGTCTATGGAGAAGCTTATGCGGGACTTCATATGGAATGGTGGGATTTCTAGATCGGTGGGGAATTTGGTGAAGTGGTCTTGGTCGGCTTTGCCTCATACACAGGGGGGCCTTGGAATTGGTTCTTTGAGGCTAAATAACAATGCTCTATTAATGAAATGGTTATGGCAGTTCACAGTGGAAAGAGATAGCCTTTGGAGACGGGTGATAGCCACTATTTACGTTGTGGAGTCTTTTGGATGGTGCACAAAACCATCGGATGGTTCTTCTATTGGAAGACCGTGGAGGGACATACAAGAGAACCTTAGAGATTTTCAAAAGTTCTCCTTCTTCAAAGTATCCTCAGGTATGTCCATTGCGGATTGTTGGTGTGCAGAGGCCTCAACTTGGAATCTTCGTTTCAGAAGAAATCTCTTTGAAAGGGAAATGAGTAGATGGGCAAACTTCACTGAAAAAATAGAAGGTGCAGCTATCGGAAATGGAAGGGACATTTTATGGTGGAAAGCAGACCCATCCGGACGTTTCACAGTCAATTCAGCCTTCATGGCTCTCACGTCCCCGTCTCCTAAACTTAATTCAGCTACAGCAAACTTGATATGGAATTTTAAAGTCCCAAAGAAGTGTATGAGCAACAATGAAACAGTGGACCACCTTTTTCTCCATTGCCCTTTTGTTTCCAAAGCTTGGGGGTTTATAGCTTCTTTGCTAGATTTATCTCTCTGCATACCTAGAAGAATTGAGGATTTCATAGCCGAAGGTTATGGAGGAAGGCAGCTCAAGGCCAAGGCTTGGGTTCTTTGGAATTGTGCGGCCCGGGCTCTTTCATGGTCGATATGGAAGGAACGAAATAGTCAACTTTTTGAAGATAAGTTGATTTTGTTCGATTCTTTTTGTGTAAATACTAAGCATCTAGCTTCTTGGTGGGCTTTGCTATACAAGAAAGTTTTTTGTAACTACAATCTTCCTTTGCTTAGACAAGATTGGAAAGTGGTGATTGTATAG

Protein sequence

MHKRLQKWRSLLLSKGGRLTLAQSVLNSIPIYFFSILKAPETIIKSMEKLMRDFIWNGGISRSVGNLVKWSWSALPHTQGGLGIGSLRLNNNALLMKWLWQFTVERDSLWRRVIATIYVVESFGWCTKPSDGSSIGRPWRDIQENLRDFQKFSFFKVSSGMSIADCWCAEASTWNLRFRRNLFEREMSRWANFTEKIEGAAIGNGRDILWWKADPSGRFTVNSAFMALTSPSPKLNSATANLIWNFKVPKKCMSNNETVDHLFLHCPFVSKAWGFIASLLDLSLCIPRRIEDFIAEGYGGRQLKAKAWVLWNCAARALSWSIWKERNSQLFEDKLILFDSFCVNTKHLASWWALLYKKVFCNYNLPLLRQDWKVVIV
Homology
BLAST of Moc04g27770 vs. NCBI nr
Match: KAB2623078.1 (ribonuclease H protein [Pyrus ussuriensis x Pyrus communis])

HSP 1 Score: 231.1 bits (588), Expect = 1.6e-56
Identity = 141/447 (31.54%), Postives = 204/447 (45.64%), Query Frame = 0

Query: 1   MHKRLQKWRSLLLSKGGRLTLAQSVLNSIPIYFFSILKAPETIIKSMEKLMRDFIWNGGI 60
           M KRLQ W+   LS+GGRLTL QSVL S+PIY+ S+ K P  +I  +EKLM+ F+W G  
Sbjct: 1   MEKRLQNWKKAFLSRGGRLTLIQSVLGSLPIYYMSLFKIPCGVIGRLEKLMKGFLWEGVE 60

Query: 61  SRSVGNLVKWSWSALPHTQGGLGIGSLRLNNNALLMKWLWQFTVERDSLWRRVIATIYVV 120
                +LVKW        +GGLG+G+LR  N ALL KWLW+F  E +SLW +VI T Y +
Sbjct: 61  EGKKNHLVKWEIVIKSKEEGGLGVGNLRNQNEALLAKWLWRFPKEPNSLWHKVIRTKYGL 120

Query: 121 ESFGWCTKPSDGSSIGRPWRDIQENLRDFQKFSFFKVSSGMSIADCW------------- 180
           +  GW   P    S   PW+DI   L+ F +   FKV +G  +  CW             
Sbjct: 121 QDNGWNANPPVRGSSRSPWKDISCGLQSFLQCCKFKVGNGERV-KCWEDGWLEGGPLKEQ 180

Query: 181 ---------------------CAEASTWNLRFRRNLFEREMSRWANFTEKIEGAAIGNGR 240
                                     +WN  FRRNL E E+   A   +K+E   +   R
Sbjct: 181 FPRLFLLSRRHNQNIASFVDLSINFLSWNFDFRRNLNEVEIEEAARLLQKVENVRLSQSR 240

Query: 241 -DILWWKADPSGRFTVNSAFMALTSPSPKLNSATANLIWNFKVPKK-------------- 300
            D   WK D SG FT  S    L++     +   ++ IW  KVP K              
Sbjct: 241 ADDRRWKLDLSGLFTCKSYRSFLSNNGIVQHFPPSSQIWKSKVPPKVKVLVWLVAIGRLN 300

Query: 301 ---------------------CMSNNETVDHLFLHCPFVSKAWGFIASLLDLSLCIPRRI 360
                                C +  E+V+H+FLHC +  + W  +   + +S  IP+  
Sbjct: 301 TCDQIQRRSPFICISPHWCSLCKAKEESVNHIFLHCSYTIQLWWKLFQEVRVSWVIPKGC 360

Query: 361 EDFIAEGYGGRQLKAKAWVLWNCAARALSWSIWKERNSQLFEDKL-ILFDSFCVNTKHLA 377
            + ++  +    +  KA  LW C   A+ W+IW ERN ++F+D   +         ++ A
Sbjct: 361 FELLSTNFEALGIGRKAKALWGCLVSAVFWNIWLERNKRIFDDYTGVGVADLWGRVRYWA 420

BLAST of Moc04g27770 vs. NCBI nr
Match: RVW80860.1 (putative ribonuclease H protein [Vitis vinifera])

HSP 1 Score: 226.9 bits (577), Expect = 3.0e-55
Identity = 142/442 (32.13%), Postives = 194/442 (43.89%), Query Frame = 0

Query: 3   KRLQKWRSLLLSKGGRLTLAQSVLNSIPIYFFSILKAPETIIKSMEKLMRDFIWNGGISR 62
           +RL  W+   LS GGR+TL QS L  +P YF S+ K P ++   +E++ RDF+W+G    
Sbjct: 179 RRLDGWQKAYLSFGGRITLIQSCLTHMPCYFLSLFKIPASVAAKIERMQRDFLWSGVGEG 238

Query: 63  SVGNLVKWSWSALPHTQGGLGIGSLRLNNNALLMKWLWQFTVERDSLWRRVIATIYVVES 122
              +LV W     P ++GGLG G + + N ALL KWLW++  E  +LW +VI +IY   S
Sbjct: 239 KRDHLVNWDVVCKPKSRGGLGFGKISIRNVALLGKWLWRYPREGSALWHQVILSIYGSHS 298

Query: 123 FGWCTKPSDGSSIGRPWRDIQENLRDFQKFSFFKVSSGMSIA---DCWCAEAS------- 182
            GW        S   PW+ I    ++F KF+ F V +G  I    D W  E         
Sbjct: 299 NGWDVNNIVRWSHRCPWKAIALVYQEFSKFTRFVVGNGDRIRFWDDLWWGEQPLGVQYPR 358

Query: 183 ----------------------TWNLRFRRNLFEREMSRWANFTEKIEGAAIGNG-RDIL 242
                                 +WN  FRRNL + E+       +  +   I +   D  
Sbjct: 359 LLRVVTDKNAPISSILGYTRPFSWNFTFRRNLSDSEIEDLEGLMQSFDRLHISSSVPDKR 418

Query: 243 WWKADPSGRFTVNSAFMALTSPSPKLNSATANLIWNFKVP-------------------- 302
            W   PSG FTV S F+AL+  S          +WN +VP                    
Sbjct: 419 SWSLSPSGLFTVKSFFLALSQYSESPPIFPTKFVWNAQVPFKVKSFVWLVAHKKVNTNDL 478

Query: 303 ---------------KKCMSNNETVDHLFLHCPFVSKAWGFIASLLDLSLCIPRRIEDFI 362
                          K CM + ETVDHLFLHC      W  +     +    PR I D +
Sbjct: 479 LQLRRPYKALSPDICKLCMKHGETVDHLFLHCSLTIGLWHRLFQSAKMDWVSPRSISDML 538

Query: 363 AEGYGGRQLKAKAWVLWNCAARALSWSIWKERNSQLFEDKL----ILFDSFCVNTKHLAS 373
           A  + G     +  VLW  A  AL W +W+ERN+++FEDK      L+DS C     L S
Sbjct: 539 ASNFNGFGFSKRGIVLWQNACIALMWVVWRERNARIFEDKARNSEYLWDSIC----FLTS 598

BLAST of Moc04g27770 vs. NCBI nr
Match: RVW52490.1 (putative ribonuclease H protein [Vitis vinifera])

HSP 1 Score: 226.1 bits (575), Expect = 5.1e-55
Identity = 143/445 (32.13%), Postives = 195/445 (43.82%), Query Frame = 0

Query: 3   KRLQKWRSLLLSKGGRLTLAQSVLNSIPIYFFSILKAPETIIKSMEKLMRDFIWNGGISR 62
           +RL  W+   LS GGR+TL QS L  +P YF S+ K P ++   +E++ R+F+W+G    
Sbjct: 21  RRLDGWQKAYLSFGGRITLIQSCLKHMPCYFLSLFKIPASVAAKIERMQREFLWSGVGEG 80

Query: 63  SVGNLVKWSWSALPHTQGGLGIGSLRLNNNALLMKWLWQFTVERDSLWRRVIATIYVVES 122
              +LV W     P ++GGLG G + + N ALL KWLW++  E  +LW +VI +IY   S
Sbjct: 81  KRDHLVNWDVVCKPKSRGGLGFGKISMRNVALLGKWLWRYPREGSALWHQVILSIYGSHS 140

Query: 123 FGWCTKPSDGSSIGRPWRDIQENLRDFQKFSFFKVSSGMSIA---DCWCAEAS------- 182
            GW    +   S   PW+ I    ++F KF+ F V  G  I    D W  +         
Sbjct: 141 NGWDVNNNVRWSHRCPWKAIALVFQEFSKFTRFVVGDGDRIRFWDDLWWGDQPLGTQYPR 200

Query: 183 ----------------------TWNLRFRRNLFEREMSRWANFTEKIEGAAIG-NGRDIL 242
                                 +WN  FRRNL + E+    +    ++   I  +  D  
Sbjct: 201 LLSVVTDKNAPISSILGYSRPFSWNFNFRRNLTDSEIEDLESLMRSLDRLHISPSVPDKR 260

Query: 243 WWKADPSGRFTVNSAFMALTSPSPKLNSATANLIWNFKVP-------------------- 302
            W   PSG FTV S F+AL+  S          +WN +VP                    
Sbjct: 261 SWSISPSGLFTVKSFFLALSQYSESPPVFPTKFVWNSQVPFKVKSFVWLVAHKKLNTNDL 320

Query: 303 ---------------KKCMSNNETVDHLFLHCPFVSKAWGFIASLLDLSLCIPRRIEDFI 362
                          K CM + ETVDHLFLHC      W  +  L  +    PR I D  
Sbjct: 321 LQLRRPHKALSPNICKLCMKHGETVDHLFLHCSLTKGLWHRLFQLAKMDWVSPRSISDMF 380

Query: 363 AEGYGGRQLKAKAWVLWNCAARALSWSIWKERNSQLFEDKL----ILFDSFCVNTKHLAS 376
              + G     K  VLW  A  AL W +W+ERN+++FEDK      L+DS C     LAS
Sbjct: 381 FTNFNGFGSSKKGVVLWQDACIALMWVVWRERNARIFEDKARNSGFLWDSIC----FLAS 440

BLAST of Moc04g27770 vs. NCBI nr
Match: CAN65484.1 (hypothetical protein VITISV_029474 [Vitis vinifera])

HSP 1 Score: 225.3 bits (573), Expect = 8.7e-55
Identity = 140/442 (31.67%), Postives = 195/442 (44.12%), Query Frame = 0

Query: 3    KRLQKWRSLLLSKGGRLTLAQSVLNSIPIYFFSILKAPETIIKSMEKLMRDFIWNGGISR 62
            +RL  W+   LS GGR+TL QS L  +P YF S+ K P ++   +E++ RDF+W+G    
Sbjct: 1441 RRLDGWQKAYLSFGGRITLIQSCLTHMPCYFLSLFKIPASVAAKIERMQRDFLWSGVGEG 1500

Query: 63   SVGNLVKWSWSALPHTQGGLGIGSLRLNNNALLMKWLWQFTVERDSLWRRVIATIYVVES 122
               +LV W     P ++GGLG G + + N ALL KWLW++  E  +LW +VI +IY   S
Sbjct: 1501 KRDHLVNWDVVCKPKSRGGLGFGKISIRNVALLGKWLWRYPREGSALWHQVILSIYGSHS 1560

Query: 123  FGWCTKPSDGSSIGRPWRDIQENLRDFQKFSFFKVSSGMSIA---DCWCAEAS------- 182
             GW        S   PW+ I    ++F KF+ F V +G  I    D W  E         
Sbjct: 1561 NGWDVNNIVRWSHRCPWKAIALVYQEFSKFTRFVVGNGDRIRFWDDLWWGEQPLGVQYPR 1620

Query: 183  ----------------------TWNLRFRRNLFEREMSRWANFTEKIEGAAIGNG-RDIL 242
                                  +WN  FRRNL + E+       + ++   I +   D  
Sbjct: 1621 LLRVVTDKNAPISSILGYTRPFSWNFTFRRNLSDSEIEDLEGLMQSLDRLHISSSVPDKR 1680

Query: 243  WWKADPSGRFTVNSAFMALTSPSPKLNSATANLIWNFKVP-------------------- 302
             W   PSG FTV S F+AL+  S          +WN +VP                    
Sbjct: 1681 SWFLSPSGLFTVKSFFLALSQYSESPTIFPTKFVWNAQVPFKVKSFVWLVAHKKVNTNDL 1740

Query: 303  ---------------KKCMSNNETVDHLFLHCPFVSKAWGFIASLLDLSLCIPRRIEDFI 362
                           K CM + ETVDHLFLHC      W  +     +    PR I D +
Sbjct: 1741 LQLRRPYKALSPDICKLCMKHGETVDHLFLHCSLTIGLWHRLFQSAKMDWVSPRSISDML 1800

Query: 363  AEGYGGRQLKAKAWVLWNCAARALSWSIWKERNSQLFEDKL----ILFDSFCVNTKHLAS 373
            +  + G     +  VLW  A  A+ W +W+ERN+++FEDK      L+DS C     L S
Sbjct: 1801 SSNFNGFGFSKRGIVLWQNACIAIMWVVWRERNARIFEDKARNSEYLWDSIC----FLTS 1860

BLAST of Moc04g27770 vs. NCBI nr
Match: CAN72359.1 (hypothetical protein VITISV_000131 [Vitis vinifera])

HSP 1 Score: 224.9 bits (572), Expect = 1.1e-54
Identity = 141/442 (31.90%), Postives = 193/442 (43.67%), Query Frame = 0

Query: 3   KRLQKWRSLLLSKGGRLTLAQSVLNSIPIYFFSILKAPETIIKSMEKLMRDFIWNGGISR 62
           +RL  W+   LS GGR+TL QS L  +P YF S+ K P ++   +E++ RDF+W+G    
Sbjct: 331 RRLDGWQKAYLSFGGRITLIQSCLTHMPCYFLSLFKIPASVAAKIERMQRDFLWSGVGEG 390

Query: 63  SVGNLVKWSWSALPHTQGGLGIGSLRLNNNALLMKWLWQFTVERDSLWRRVIATIYVVES 122
              +LV W     P ++GGLG G + + N ALL KWLW++  E  +LW +VI +IY   S
Sbjct: 391 KRDHLVNWDVVCKPKSRGGLGFGKISIRNVALLGKWLWRYPREGSALWHQVILSIYGSHS 450

Query: 123 FGWCTKPSDGSSIGRPWRDIQENLRDFQKFSFFKVSSGMSIA---DCWCAEAS------- 182
            GW        S   PW+ I    ++F KF+ F V +G  I    D W  E         
Sbjct: 451 NGWDVNNIVRWSHRCPWKAIALVYQEFSKFTRFVVGNGDRIRFWDDLWWGEQPLGVQYPR 510

Query: 183 ----------------------TWNLRFRRNLFEREMSRWANFTEKIEGAAIGNG-RDIL 242
                                 +WN  FRRNL + E+       +  +   I +   D  
Sbjct: 511 LLRVVTDKNAPISSILGSTRPFSWNFTFRRNLSDSEIEDLEGLMQSFDRLHISSSVPDKR 570

Query: 243 WWKADPSGRFTVNSAFMALTSPSPKLNSATANLIWNFKVP-------------------- 302
            W   PSG FTV   F+AL+  S          +WN +VP                    
Sbjct: 571 SWSLSPSGLFTVKXFFLALSQYSESPPIFPTKFVWNAQVPFKVKSFVWLVAHKKVNTNDL 630

Query: 303 ---------------KKCMSNNETVDHLFLHCPFVSKAWGFIASLLDLSLCIPRRIEDFI 362
                          K CM + ETVDHLFLHC      W  +     +    PR I D +
Sbjct: 631 LQLRRPYKALSPDICKLCMKHGETVDHLFLHCSLTIGLWHRLFQSAKMDWVSPRSISDML 690

Query: 363 AEGYGGRQLKAKAWVLWNCAARALSWSIWKERNSQLFEDKL----ILFDSFCVNTKHLAS 373
           A  + G     +  VLW  A  AL W +W+ERN+++FEDK      L+DS C     L S
Sbjct: 691 ASNFNGFGFSKRGIVLWQNACIALMWVVWRERNARIFEDKARNSEYLWDSIC----FLTS 750

BLAST of Moc04g27770 vs. ExPASy Swiss-Prot
Match: P0C2F6 (Putative ribonuclease H protein At1g65750 OS=Arabidopsis thaliana OX=3702 GN=At1g65750 PE=3 SV=1)

HSP 1 Score: 108.2 bits (269), Expect = 2.0e-22
Identity = 80/281 (28.47%), Postives = 128/281 (45.55%), Query Frame = 0

Query: 4   RLQKWRSLLLSKGGRLTLAQSVLNSIPIYFFSILKAPETIIKSMEKLMRDFIWNGGISRS 63
           R+  WR   LS  GRLTL ++VL+S+P++  S +  P++I+  +++L R F+W     + 
Sbjct: 23  RMSGWREKTLSFAGRLTLTKAVLSSMPVHSMSTILLPQSILNRLDQLSRTFLWGSTAEKK 82

Query: 64  VGNLVKWSWSALPHTQGGLGIGSLRLNNNALLMKWLWQFTVERDSLWRRVIATIY---VV 123
             +LVKWS    P  +GGLG+ + +  N AL+ K  W+   E++SLW  V+   Y    +
Sbjct: 83  KQHLVKWSKVCSPKKEGGLGVRAAKSMNRALISKVGWRLLQEKNSLWTLVLQKKYHVGEI 142

Query: 124 ESFGWCTKPSDGSSIGRPWRDIQENLRDF------------QKFSFF-----------KV 183
               W       SS    WR I   LRD             Q+  F+           ++
Sbjct: 143 RDSRWLIPKGSWSS---TWRSIAIGLRDVVSHGVGWIPGDGQQIRFWTDRWVSGKPLLEL 202

Query: 184 SSGMSIADCWCAEASTWNLRFRRNLFEREMSRWANFTEKIEGAA-----IGNGRDILWWK 243
            +G    DC    A    +  R   F + +  +     ++E  A     +   RD L WK
Sbjct: 203 DNGERPTDCDTVVAKDLWIPGRGWDFAK-IDPYTTNNTRLELRAVVLDLVTGARDRLSWK 262

Query: 244 ADPSGRFTVNSAFMALT-SPSPKLNSAT-ANLIWNFKVPKK 252
               G+F+V SA+  LT    P+ N A+  N +W  +VP++
Sbjct: 263 FSQDGQFSVRSAYEMLTVDEVPRPNMASFFNCLWKVRVPER 299

BLAST of Moc04g27770 vs. ExPASy Swiss-Prot
Match: P08548 (LINE-1 reverse transcriptase homolog OS=Nycticebus coucang OX=9470 PE=4 SV=1)

HSP 1 Score: 46.2 bits (108), Expect = 9.4e-04
Identity = 29/112 (25.89%), Postives = 57/112 (50.89%), Query Frame = 0

Query: 5   LQKWRSLLLSKGGRLTLAQSVLNSIPIYFFSI--LKAPETIIKSMEKLMRDFIWNGGISR 64
           + KW+++  S  GR+ + +  +    IY F+   +KAP +  K +EK++  FIWN    +
Sbjct: 795 VNKWKNIPCSWLGRINIVKMSILPKAIYNFNAIPIKAPLSYFKDLEKIILHFIWNQKKPQ 854

Query: 65  SVGNLVKWSWSALPHTQGGLGIGSLRLNNNALLMKWLWQFTVERD-SLWRRV 114
               L+     +  +  GG+ +  LRL   ++++K  W +   R+  +W R+
Sbjct: 855 IAKTLL-----SNKNKAGGITLPDLRLYYKSIVIKTAWYWHKNREVDVWNRI 901

BLAST of Moc04g27770 vs. ExPASy TrEMBL
Match: A0A803P465 (Uncharacterized protein OS=Cannabis sativa OX=3483 PE=4 SV=1)

HSP 1 Score: 233.0 bits (593), Expect = 2.0e-57
Identity = 138/404 (34.16%), Postives = 190/404 (47.03%), Query Frame = 0

Query: 3    KRLQKWRSLLLSKGGRLTLAQSVLNSIPIYFFSILKAPETIIKSMEKLMRDFIWNGGISR 62
            KRL  W+   LSKGGRLTL QSVL+S+P+YF S+ KAP ++ K++EK+MRDF+W G  S 
Sbjct: 1478 KRLDGWKCAFLSKGGRLTLIQSVLSSLPLYFLSLFKAPRSVTKALEKMMRDFLWEGSESS 1537

Query: 63   SVGNLVKWSWSALPHTQGGLGIGSLRLNNNALLMKWLWQFTVERDSLWRRVIATIYVVES 122
               +LV W     P  +GGLGIG L L N +LLMKWLW+F +E++SLW RV+ + Y    
Sbjct: 1538 GGEHLVAWDEVCRPRHEGGLGIGRLELRNKSLLMKWLWRFPLEQNSLWHRVVVSRYGRAD 1597

Query: 123  FGWCTKPSDGSSIGRPWRDIQENLRDFQKFSFFKVSSGMSI---ADCWCAE--------- 182
              W +K     S   PWRDI +   +F     FK+  G  I    D W  +         
Sbjct: 1598 NLWDSKKGSRLSPKGPWRDISDLYDEFLGLVHFKLGRGDRIRFWEDVWIDDYPLVSAFPD 1657

Query: 183  -------------------------ASTWNLRFRRNLFEREMSRWANFTEKIEGA-AIGN 242
                                       +WN +FRRNL +RE+    +  +K+E    +  
Sbjct: 1658 LAVISEGRNTPIKELIVDEGLPGGWVESWNFKFRRNLMDRELPSLISLMQKVEHVRVLSI 1717

Query: 243  GRDILWWKADPSGRFTVNSAFMALTS-PSPKLNSATANLIWNFKVPKK------------ 302
              D   WK DPSG F+  SAF  + S PS ++   T +L W    P K            
Sbjct: 1718 SEDSRIWKPDPSGVFSCKSAFSWMVSNPSFRVLDWTKSL-WKSCAPSKVKVFGWLLALEK 1777

Query: 303  -----------------------CMSNNETVDHLFLHCPFVSKAWGFIASLLDLSLCIPR 333
                                   C  + E+V HLFL C F+ + W ++     LS  +PR
Sbjct: 1778 VNVHDKVQKRKPFLYISPGWCVCCKGSGESVGHLFLECKFIGRIWRYVLGEFGLSWVMPR 1837

BLAST of Moc04g27770 vs. ExPASy TrEMBL
Match: A0A5N5H648 (Ribonuclease H protein OS=Pyrus ussuriensis x Pyrus communis OX=2448454 GN=D8674_025260 PE=4 SV=1)

HSP 1 Score: 231.1 bits (588), Expect = 7.6e-57
Identity = 141/447 (31.54%), Postives = 204/447 (45.64%), Query Frame = 0

Query: 1   MHKRLQKWRSLLLSKGGRLTLAQSVLNSIPIYFFSILKAPETIIKSMEKLMRDFIWNGGI 60
           M KRLQ W+   LS+GGRLTL QSVL S+PIY+ S+ K P  +I  +EKLM+ F+W G  
Sbjct: 1   MEKRLQNWKKAFLSRGGRLTLIQSVLGSLPIYYMSLFKIPCGVIGRLEKLMKGFLWEGVE 60

Query: 61  SRSVGNLVKWSWSALPHTQGGLGIGSLRLNNNALLMKWLWQFTVERDSLWRRVIATIYVV 120
                +LVKW        +GGLG+G+LR  N ALL KWLW+F  E +SLW +VI T Y +
Sbjct: 61  EGKKNHLVKWEIVIKSKEEGGLGVGNLRNQNEALLAKWLWRFPKEPNSLWHKVIRTKYGL 120

Query: 121 ESFGWCTKPSDGSSIGRPWRDIQENLRDFQKFSFFKVSSGMSIADCW------------- 180
           +  GW   P    S   PW+DI   L+ F +   FKV +G  +  CW             
Sbjct: 121 QDNGWNANPPVRGSSRSPWKDISCGLQSFLQCCKFKVGNGERV-KCWEDGWLEGGPLKEQ 180

Query: 181 ---------------------CAEASTWNLRFRRNLFEREMSRWANFTEKIEGAAIGNGR 240
                                     +WN  FRRNL E E+   A   +K+E   +   R
Sbjct: 181 FPRLFLLSRRHNQNIASFVDLSINFLSWNFDFRRNLNEVEIEEAARLLQKVENVRLSQSR 240

Query: 241 -DILWWKADPSGRFTVNSAFMALTSPSPKLNSATANLIWNFKVPKK-------------- 300
            D   WK D SG FT  S    L++     +   ++ IW  KVP K              
Sbjct: 241 ADDRRWKLDLSGLFTCKSYRSFLSNNGIVQHFPPSSQIWKSKVPPKVKVLVWLVAIGRLN 300

Query: 301 ---------------------CMSNNETVDHLFLHCPFVSKAWGFIASLLDLSLCIPRRI 360
                                C +  E+V+H+FLHC +  + W  +   + +S  IP+  
Sbjct: 301 TCDQIQRRSPFICISPHWCSLCKAKEESVNHIFLHCSYTIQLWWKLFQEVRVSWVIPKGC 360

Query: 361 EDFIAEGYGGRQLKAKAWVLWNCAARALSWSIWKERNSQLFEDKL-ILFDSFCVNTKHLA 377
            + ++  +    +  KA  LW C   A+ W+IW ERN ++F+D   +         ++ A
Sbjct: 361 FELLSTNFEALGIGRKAKALWGCLVSAVFWNIWLERNKRIFDDYTGVGVADLWGRVRYWA 420

BLAST of Moc04g27770 vs. ExPASy TrEMBL
Match: A0A438H8I3 (Putative ribonuclease H protein OS=Vitis vinifera OX=29760 GN=VvCHDp000001_629 PE=4 SV=1)

HSP 1 Score: 226.9 bits (577), Expect = 1.4e-55
Identity = 142/442 (32.13%), Postives = 194/442 (43.89%), Query Frame = 0

Query: 3   KRLQKWRSLLLSKGGRLTLAQSVLNSIPIYFFSILKAPETIIKSMEKLMRDFIWNGGISR 62
           +RL  W+   LS GGR+TL QS L  +P YF S+ K P ++   +E++ RDF+W+G    
Sbjct: 179 RRLDGWQKAYLSFGGRITLIQSCLTHMPCYFLSLFKIPASVAAKIERMQRDFLWSGVGEG 238

Query: 63  SVGNLVKWSWSALPHTQGGLGIGSLRLNNNALLMKWLWQFTVERDSLWRRVIATIYVVES 122
              +LV W     P ++GGLG G + + N ALL KWLW++  E  +LW +VI +IY   S
Sbjct: 239 KRDHLVNWDVVCKPKSRGGLGFGKISIRNVALLGKWLWRYPREGSALWHQVILSIYGSHS 298

Query: 123 FGWCTKPSDGSSIGRPWRDIQENLRDFQKFSFFKVSSGMSIA---DCWCAEAS------- 182
            GW        S   PW+ I    ++F KF+ F V +G  I    D W  E         
Sbjct: 299 NGWDVNNIVRWSHRCPWKAIALVYQEFSKFTRFVVGNGDRIRFWDDLWWGEQPLGVQYPR 358

Query: 183 ----------------------TWNLRFRRNLFEREMSRWANFTEKIEGAAIGNG-RDIL 242
                                 +WN  FRRNL + E+       +  +   I +   D  
Sbjct: 359 LLRVVTDKNAPISSILGYTRPFSWNFTFRRNLSDSEIEDLEGLMQSFDRLHISSSVPDKR 418

Query: 243 WWKADPSGRFTVNSAFMALTSPSPKLNSATANLIWNFKVP-------------------- 302
            W   PSG FTV S F+AL+  S          +WN +VP                    
Sbjct: 419 SWSLSPSGLFTVKSFFLALSQYSESPPIFPTKFVWNAQVPFKVKSFVWLVAHKKVNTNDL 478

Query: 303 ---------------KKCMSNNETVDHLFLHCPFVSKAWGFIASLLDLSLCIPRRIEDFI 362
                          K CM + ETVDHLFLHC      W  +     +    PR I D +
Sbjct: 479 LQLRRPYKALSPDICKLCMKHGETVDHLFLHCSLTIGLWHRLFQSAKMDWVSPRSISDML 538

Query: 363 AEGYGGRQLKAKAWVLWNCAARALSWSIWKERNSQLFEDKL----ILFDSFCVNTKHLAS 373
           A  + G     +  VLW  A  AL W +W+ERN+++FEDK      L+DS C     L S
Sbjct: 539 ASNFNGFGFSKRGIVLWQNACIALMWVVWRERNARIFEDKARNSEYLWDSIC----FLTS 598

BLAST of Moc04g27770 vs. ExPASy TrEMBL
Match: A0A438EXL3 (Putative ribonuclease H protein OS=Vitis vinifera OX=29760 GN=VvCHDh000004_220 PE=4 SV=1)

HSP 1 Score: 226.1 bits (575), Expect = 2.5e-55
Identity = 143/445 (32.13%), Postives = 195/445 (43.82%), Query Frame = 0

Query: 3   KRLQKWRSLLLSKGGRLTLAQSVLNSIPIYFFSILKAPETIIKSMEKLMRDFIWNGGISR 62
           +RL  W+   LS GGR+TL QS L  +P YF S+ K P ++   +E++ R+F+W+G    
Sbjct: 21  RRLDGWQKAYLSFGGRITLIQSCLKHMPCYFLSLFKIPASVAAKIERMQREFLWSGVGEG 80

Query: 63  SVGNLVKWSWSALPHTQGGLGIGSLRLNNNALLMKWLWQFTVERDSLWRRVIATIYVVES 122
              +LV W     P ++GGLG G + + N ALL KWLW++  E  +LW +VI +IY   S
Sbjct: 81  KRDHLVNWDVVCKPKSRGGLGFGKISMRNVALLGKWLWRYPREGSALWHQVILSIYGSHS 140

Query: 123 FGWCTKPSDGSSIGRPWRDIQENLRDFQKFSFFKVSSGMSIA---DCWCAEAS------- 182
            GW    +   S   PW+ I    ++F KF+ F V  G  I    D W  +         
Sbjct: 141 NGWDVNNNVRWSHRCPWKAIALVFQEFSKFTRFVVGDGDRIRFWDDLWWGDQPLGTQYPR 200

Query: 183 ----------------------TWNLRFRRNLFEREMSRWANFTEKIEGAAIG-NGRDIL 242
                                 +WN  FRRNL + E+    +    ++   I  +  D  
Sbjct: 201 LLSVVTDKNAPISSILGYSRPFSWNFNFRRNLTDSEIEDLESLMRSLDRLHISPSVPDKR 260

Query: 243 WWKADPSGRFTVNSAFMALTSPSPKLNSATANLIWNFKVP-------------------- 302
            W   PSG FTV S F+AL+  S          +WN +VP                    
Sbjct: 261 SWSISPSGLFTVKSFFLALSQYSESPPVFPTKFVWNSQVPFKVKSFVWLVAHKKLNTNDL 320

Query: 303 ---------------KKCMSNNETVDHLFLHCPFVSKAWGFIASLLDLSLCIPRRIEDFI 362
                          K CM + ETVDHLFLHC      W  +  L  +    PR I D  
Sbjct: 321 LQLRRPHKALSPNICKLCMKHGETVDHLFLHCSLTKGLWHRLFQLAKMDWVSPRSISDMF 380

Query: 363 AEGYGGRQLKAKAWVLWNCAARALSWSIWKERNSQLFEDKL----ILFDSFCVNTKHLAS 376
              + G     K  VLW  A  AL W +W+ERN+++FEDK      L+DS C     LAS
Sbjct: 381 FTNFNGFGSSKKGVVLWQDACIALMWVVWRERNARIFEDKARNSGFLWDSIC----FLAS 440

BLAST of Moc04g27770 vs. ExPASy TrEMBL
Match: A5BCI7 (Reverse transcriptase domain-containing protein OS=Vitis vinifera OX=29760 GN=VITISV_029474 PE=4 SV=1)

HSP 1 Score: 225.3 bits (573), Expect = 4.2e-55
Identity = 140/442 (31.67%), Postives = 195/442 (44.12%), Query Frame = 0

Query: 3    KRLQKWRSLLLSKGGRLTLAQSVLNSIPIYFFSILKAPETIIKSMEKLMRDFIWNGGISR 62
            +RL  W+   LS GGR+TL QS L  +P YF S+ K P ++   +E++ RDF+W+G    
Sbjct: 1441 RRLDGWQKAYLSFGGRITLIQSCLTHMPCYFLSLFKIPASVAAKIERMQRDFLWSGVGEG 1500

Query: 63   SVGNLVKWSWSALPHTQGGLGIGSLRLNNNALLMKWLWQFTVERDSLWRRVIATIYVVES 122
               +LV W     P ++GGLG G + + N ALL KWLW++  E  +LW +VI +IY   S
Sbjct: 1501 KRDHLVNWDVVCKPKSRGGLGFGKISIRNVALLGKWLWRYPREGSALWHQVILSIYGSHS 1560

Query: 123  FGWCTKPSDGSSIGRPWRDIQENLRDFQKFSFFKVSSGMSIA---DCWCAEAS------- 182
             GW        S   PW+ I    ++F KF+ F V +G  I    D W  E         
Sbjct: 1561 NGWDVNNIVRWSHRCPWKAIALVYQEFSKFTRFVVGNGDRIRFWDDLWWGEQPLGVQYPR 1620

Query: 183  ----------------------TWNLRFRRNLFEREMSRWANFTEKIEGAAIGNG-RDIL 242
                                  +WN  FRRNL + E+       + ++   I +   D  
Sbjct: 1621 LLRVVTDKNAPISSILGYTRPFSWNFTFRRNLSDSEIEDLEGLMQSLDRLHISSSVPDKR 1680

Query: 243  WWKADPSGRFTVNSAFMALTSPSPKLNSATANLIWNFKVP-------------------- 302
             W   PSG FTV S F+AL+  S          +WN +VP                    
Sbjct: 1681 SWFLSPSGLFTVKSFFLALSQYSESPTIFPTKFVWNAQVPFKVKSFVWLVAHKKVNTNDL 1740

Query: 303  ---------------KKCMSNNETVDHLFLHCPFVSKAWGFIASLLDLSLCIPRRIEDFI 362
                           K CM + ETVDHLFLHC      W  +     +    PR I D +
Sbjct: 1741 LQLRRPYKALSPDICKLCMKHGETVDHLFLHCSLTIGLWHRLFQSAKMDWVSPRSISDML 1800

Query: 363  AEGYGGRQLKAKAWVLWNCAARALSWSIWKERNSQLFEDKL----ILFDSFCVNTKHLAS 373
            +  + G     +  VLW  A  A+ W +W+ERN+++FEDK      L+DS C     L S
Sbjct: 1801 SSNFNGFGFSKRGIVLWQNACIAIMWVVWRERNARIFEDKARNSEYLWDSIC----FLTS 1860

BLAST of Moc04g27770 vs. TAIR 10
Match: AT3G24255.1 (RNA-directed DNA polymerase (reverse transcriptase)-related family protein )

HSP 1 Score: 65.1 bits (157), Expect = 1.4e-10
Identity = 35/115 (30.43%), Postives = 58/115 (50.43%), Query Frame = 0

Query: 4   RLQKWRSLLLSKGGRLTLAQSVLNSIPIYFFSILKAPETIIKSMEKLMRDFIWNGGISRS 63
           R+ KW +  LS  GRL L  SV++S+  ++ S  + P   IK ++ +   F+W+G    +
Sbjct: 52  RIGKWTARHLSFAGRLQLISSVIHSLTNFWMSAFRLPSACIKEIDSICSSFLWSGPELNT 111

Query: 64  VGNLVKWSWSALPHTQGGLGIGSLR---------LNNNALLMKWLWQFTVERDSL 110
               V WS    P  +GGLGI SL+         ++ N  L  W+W+  ++  +L
Sbjct: 112 KKAKVAWSDVCTPKDEGGLGIRSLKEANKGSFWSISGNTTLGSWMWKKILKHRAL 166

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAB2623078.11.6e-5631.54ribonuclease H protein [Pyrus ussuriensis x Pyrus communis][more]
RVW80860.13.0e-5532.13putative ribonuclease H protein [Vitis vinifera][more]
RVW52490.15.1e-5532.13putative ribonuclease H protein [Vitis vinifera][more]
CAN65484.18.7e-5531.67hypothetical protein VITISV_029474 [Vitis vinifera][more]
CAN72359.11.1e-5431.90hypothetical protein VITISV_000131 [Vitis vinifera][more]
Match NameE-valueIdentityDescription
P0C2F62.0e-2228.47Putative ribonuclease H protein At1g65750 OS=Arabidopsis thaliana OX=3702 GN=At1... [more]
P085489.4e-0425.89LINE-1 reverse transcriptase homolog OS=Nycticebus coucang OX=9470 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
A0A803P4652.0e-5734.16Uncharacterized protein OS=Cannabis sativa OX=3483 PE=4 SV=1[more]
A0A5N5H6487.6e-5731.54Ribonuclease H protein OS=Pyrus ussuriensis x Pyrus communis OX=2448454 GN=D8674... [more]
A0A438H8I31.4e-5532.13Putative ribonuclease H protein OS=Vitis vinifera OX=29760 GN=VvCHDp000001_629 P... [more]
A0A438EXL32.5e-5532.13Putative ribonuclease H protein OS=Vitis vinifera OX=29760 GN=VvCHDh000004_220 P... [more]
A5BCI74.2e-5531.67Reverse transcriptase domain-containing protein OS=Vitis vinifera OX=29760 GN=VI... [more]
Match NameE-valueIdentityDescription
AT3G24255.11.4e-1030.43RNA-directed DNA polymerase (reverse transcriptase)-related family protein [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (OHB3-1) v2
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availablePANTHERPTHR33116:SF33OS01G0885550 PROTEINcoord: 3..285
NoneNo IPR availablePANTHERPTHR33116REVERSE TRANSCRIPTASE ZINC-BINDING DOMAIN-CONTAINING PROTEIN-RELATED-RELATEDcoord: 3..285

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Moc04g27770.1Moc04g27770.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0110165 cellular anatomical entity