Moc04g27390 (gene) Bitter gourd (OHB3-1) v2

Overview
NameMoc04g27390
Typegene
OrganismMomordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
Descriptionprotein PIN-LIKES 2-like
Locationchr4: 20226630 .. 20227991 (+)
RNA-Seq ExpressionMoc04g27390
SyntenyMoc04g27390
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGGTGATTACTTCAGTGTCATAGATCGGAGTGGAGTGAGGTCTAGTAGTGAAGATCTGCTTAGTGCAATTACGCCATTGATGAAGCTTCTCTCGCTCACAGCAATAGGGCTACTTCTTTCTCACCCAAAAGTCCAAATGATTCCAAGAGCAACCCTAAGGCTCCTGAGCAAACTCGTTTTTGCCCTGTTCTTGCCCTGCCTCATCTTCACCCATCTGGGTGAGAGCATCACGCTTCAAAACATTGCTAAATGGTGGTTTATCCCTGTGAATGTTTTGATTAGTACAGGTATTGGGTGCCTTCTAGGGTTCTTAGTGGTGATTCTATGCCGCCCCCCTCCTCGGTTGATGCGGTTCACCGTTATCTCGACTGCGTTCGGCAACACCGGAAACCTCCCTCTTGCCATTGTTGGTTCTGTCTGTCATACTGCTGATAACCCCTTTGGCCAGCATTGTCACTCTAGAGGGGTTTCTTATGTTTCCTTTTGCCAGTGGGTTTCTGTTATTCTTGCTTATACTCTTGTTTATCATATGATGGAGCCTCCATTGGAGTTTTATGAGATTGTTGAGGAGGGCACTGAGATTGAAGAATTGGAACAAATTAGTGACAATGATGCTAGTAAGCCTCTCCTGATCGAAGCTGAATGGCCCGGGATTGAAGAGAAAGAAACTGAGCATTGTAAAGCACCCTTCATTGCTAGGATCTTCAAGAGCATTTCAAATGTTTCCCAGTCAACTTTTCCTGACCTTGATCACTCCAGAGAAAGTAGTACTCCTACGTACCCGGAGTCAATCAGGTGTTTGGCCGAACCAAGGGTTGTGAGGAAGATCAGAATTGTAGCTGTACAAACTCCCCTAAAGCACATTCTGCAGCCACCAACCATTGCTTCTTTGTTAGCCATCATTGTTGGGTTGGTGCCCCAAATCAGAGCATCTTTCTTCGGCCCCGATGCTCCGCTATCGTTCATCTCCGATAGTTTAGAAATCCTAGCTGCTGCAGTGGTTCCTTTTGTGATGCTTATGTTGGGTGGCATGCTTGCCGAGGGGCCTCACGAGTTGTCCACACTTGGCCTACGTACTACAATCGGCATAAGCGTTGCAAGACTCTTTGTCCTCCCTATACTGGGAATCGGGATCGTCGTATCCGCAGACAAGCTGAATTTTCTTGTTGATGGTGATCCAATGTACAAATTTGTGCTGTTGTTGCAGTATACAACACCAACTGCCATTTTGTTGGGAGCAATTGCAAGTTTGAGGGGATATGCAGTGAGGGAGGCCTCAGCTCTTCTTTTTTGGGAACATATATTTGCCCTCCTCTCCCTTTCTCTCTATCTTTTTGTTTACTTTAAGATTGTGATATAA

mRNA sequence

ATGGGTGATTACTTCAGTGTCATAGATCGGAGTGGAGTGAGGTCTAGTAGTGAAGATCTGCTTAGTGCAATTACGCCATTGATGAAGCTTCTCTCGCTCACAGCAATAGGGCTACTTCTTTCTCACCCAAAAGTCCAAATGATTCCAAGAGCAACCCTAAGGCTCCTGAGCAAACTCGTTTTTGCCCTGTTCTTGCCCTGCCTCATCTTCACCCATCTGGGTGAGAGCATCACGCTTCAAAACATTGCTAAATGGTGGTTTATCCCTGTGAATGTTTTGATTAGTACAGGTATTGGGTGCCTTCTAGGGTTCTTAGTGGTGATTCTATGCCGCCCCCCTCCTCGGTTGATGCGGTTCACCGTTATCTCGACTGCGTTCGGCAACACCGGAAACCTCCCTCTTGCCATTGTTGGTTCTGTCTGTCATACTGCTGATAACCCCTTTGGCCAGCATTGTCACTCTAGAGGGGTTTCTTATGTTTCCTTTTGCCAGTGGGTTTCTGTTATTCTTGCTTATACTCTTGTTTATCATATGATGGAGCCTCCATTGGAGTTTTATGAGATTGTTGAGGAGGGCACTGAGATTGAAGAATTGGAACAAATTAGTGACAATGATGCTAGTAAGCCTCTCCTGATCGAAGCTGAATGGCCCGGGATTGAAGAGAAAGAAACTGAGCATTGTAAAGCACCCTTCATTGCTAGGATCTTCAAGAGCATTTCAAATGTTTCCCAGTCAACTTTTCCTGACCTTGATCACTCCAGAGAAAGTAGTACTCCTACGTACCCGGAGTCAATCAGGTGTTTGGCCGAACCAAGGGTTGTGAGGAAGATCAGAATTGTAGCTGTACAAACTCCCCTAAAGCACATTCTGCAGCCACCAACCATTGCTTCTTTGTTAGCCATCATTGTTGGGTTGGTGCCCCAAATCAGAGCATCTTTCTTCGGCCCCGATGCTCCGCTATCGTTCATCTCCGATAGTTTAGAAATCCTAGCTGCTGCAGTGGTTCCTTTTGTGATGCTTATGTTGGGTGGCATGCTTGCCGAGGGGCCTCACGAGTTGTCCACACTTGGCCTACGTACTACAATCGGCATAAGCGTTGCAAGACTCTTTGTCCTCCCTATACTGGGAATCGGGATCGTCGTATCCGCAGACAAGCTGAATTTTCTTGTTGATGGTGATCCAATGTACAAATTTGTGCTGTTGTTGCAGTATACAACACCAACTGCCATTTTGTTGGGAGCAATTGCAAGTTTGAGGGGATATGCAGTGAGGGAGGCCTCAGCTCTTCTTTTTTGGGAACATATATTTGCCCTCCTCTCCCTTTCTCTCTATCTTTTTGTTTACTTTAAGATTGTGATATAA

Coding sequence (CDS)

ATGGGTGATTACTTCAGTGTCATAGATCGGAGTGGAGTGAGGTCTAGTAGTGAAGATCTGCTTAGTGCAATTACGCCATTGATGAAGCTTCTCTCGCTCACAGCAATAGGGCTACTTCTTTCTCACCCAAAAGTCCAAATGATTCCAAGAGCAACCCTAAGGCTCCTGAGCAAACTCGTTTTTGCCCTGTTCTTGCCCTGCCTCATCTTCACCCATCTGGGTGAGAGCATCACGCTTCAAAACATTGCTAAATGGTGGTTTATCCCTGTGAATGTTTTGATTAGTACAGGTATTGGGTGCCTTCTAGGGTTCTTAGTGGTGATTCTATGCCGCCCCCCTCCTCGGTTGATGCGGTTCACCGTTATCTCGACTGCGTTCGGCAACACCGGAAACCTCCCTCTTGCCATTGTTGGTTCTGTCTGTCATACTGCTGATAACCCCTTTGGCCAGCATTGTCACTCTAGAGGGGTTTCTTATGTTTCCTTTTGCCAGTGGGTTTCTGTTATTCTTGCTTATACTCTTGTTTATCATATGATGGAGCCTCCATTGGAGTTTTATGAGATTGTTGAGGAGGGCACTGAGATTGAAGAATTGGAACAAATTAGTGACAATGATGCTAGTAAGCCTCTCCTGATCGAAGCTGAATGGCCCGGGATTGAAGAGAAAGAAACTGAGCATTGTAAAGCACCCTTCATTGCTAGGATCTTCAAGAGCATTTCAAATGTTTCCCAGTCAACTTTTCCTGACCTTGATCACTCCAGAGAAAGTAGTACTCCTACGTACCCGGAGTCAATCAGGTGTTTGGCCGAACCAAGGGTTGTGAGGAAGATCAGAATTGTAGCTGTACAAACTCCCCTAAAGCACATTCTGCAGCCACCAACCATTGCTTCTTTGTTAGCCATCATTGTTGGGTTGGTGCCCCAAATCAGAGCATCTTTCTTCGGCCCCGATGCTCCGCTATCGTTCATCTCCGATAGTTTAGAAATCCTAGCTGCTGCAGTGGTTCCTTTTGTGATGCTTATGTTGGGTGGCATGCTTGCCGAGGGGCCTCACGAGTTGTCCACACTTGGCCTACGTACTACAATCGGCATAAGCGTTGCAAGACTCTTTGTCCTCCCTATACTGGGAATCGGGATCGTCGTATCCGCAGACAAGCTGAATTTTCTTGTTGATGGTGATCCAATGTACAAATTTGTGCTGTTGTTGCAGTATACAACACCAACTGCCATTTTGTTGGGAGCAATTGCAAGTTTGAGGGGATATGCAGTGAGGGAGGCCTCAGCTCTTCTTTTTTGGGAACATATATTTGCCCTCCTCTCCCTTTCTCTCTATCTTTTTGTTTACTTTAAGATTGTGATATAA

Protein sequence

MGDYFSVIDRSGVRSSSEDLLSAITPLMKLLSLTAIGLLLSHPKVQMIPRATLRLLSKLVFALFLPCLIFTHLGESITLQNIAKWWFIPVNVLISTGIGCLLGFLVVILCRPPPRLMRFTVISTAFGNTGNLPLAIVGSVCHTADNPFGQHCHSRGVSYVSFCQWVSVILAYTLVYHMMEPPLEFYEIVEEGTEIEELEQISDNDASKPLLIEAEWPGIEEKETEHCKAPFIARIFKSISNVSQSTFPDLDHSRESSTPTYPESIRCLAEPRVVRKIRIVAVQTPLKHILQPPTIASLLAIIVGLVPQIRASFFGPDAPLSFISDSLEILAAAVVPFVMLMLGGMLAEGPHELSTLGLRTTIGISVARLFVLPILGIGIVVSADKLNFLVDGDPMYKFVLLLQYTTPTAILLGAIASLRGYAVREASALLFWEHIFALLSLSLYLFVYFKIVI
Homology
BLAST of Moc04g27390 vs. NCBI nr
Match: XP_022152345.1 (protein PIN-LIKES 2-like [Momordica charantia])

HSP 1 Score: 866.7 bits (2238), Expect = 8.9e-248
Identity = 453/453 (100.00%), Postives = 453/453 (100.00%), Query Frame = 0

Query: 1   MGDYFSVIDRSGVRSSSEDLLSAITPLMKLLSLTAIGLLLSHPKVQMIPRATLRLLSKLV 60
           MGDYFSVIDRSGVRSSSEDLLSAITPLMKLLSLTAIGLLLSHPKVQMIPRATLRLLSKLV
Sbjct: 1   MGDYFSVIDRSGVRSSSEDLLSAITPLMKLLSLTAIGLLLSHPKVQMIPRATLRLLSKLV 60

Query: 61  FALFLPCLIFTHLGESITLQNIAKWWFIPVNVLISTGIGCLLGFLVVILCRPPPRLMRFT 120
           FALFLPCLIFTHLGESITLQNIAKWWFIPVNVLISTGIGCLLGFLVVILCRPPPRLMRFT
Sbjct: 61  FALFLPCLIFTHLGESITLQNIAKWWFIPVNVLISTGIGCLLGFLVVILCRPPPRLMRFT 120

Query: 121 VISTAFGNTGNLPLAIVGSVCHTADNPFGQHCHSRGVSYVSFCQWVSVILAYTLVYHMME 180
           VISTAFGNTGNLPLAIVGSVCHTADNPFGQHCHSRGVSYVSFCQWVSVILAYTLVYHMME
Sbjct: 121 VISTAFGNTGNLPLAIVGSVCHTADNPFGQHCHSRGVSYVSFCQWVSVILAYTLVYHMME 180

Query: 181 PPLEFYEIVEEGTEIEELEQISDNDASKPLLIEAEWPGIEEKETEHCKAPFIARIFKSIS 240
           PPLEFYEIVEEGTEIEELEQISDNDASKPLLIEAEWPGIEEKETEHCKAPFIARIFKSIS
Sbjct: 181 PPLEFYEIVEEGTEIEELEQISDNDASKPLLIEAEWPGIEEKETEHCKAPFIARIFKSIS 240

Query: 241 NVSQSTFPDLDHSRESSTPTYPESIRCLAEPRVVRKIRIVAVQTPLKHILQPPTIASLLA 300
           NVSQSTFPDLDHSRESSTPTYPESIRCLAEPRVVRKIRIVAVQTPLKHILQPPTIASLLA
Sbjct: 241 NVSQSTFPDLDHSRESSTPTYPESIRCLAEPRVVRKIRIVAVQTPLKHILQPPTIASLLA 300

Query: 301 IIVGLVPQIRASFFGPDAPLSFISDSLEILAAAVVPFVMLMLGGMLAEGPHELSTLGLRT 360
           IIVGLVPQIRASFFGPDAPLSFISDSLEILAAAVVPFVMLMLGGMLAEGPHELSTLGLRT
Sbjct: 301 IIVGLVPQIRASFFGPDAPLSFISDSLEILAAAVVPFVMLMLGGMLAEGPHELSTLGLRT 360

Query: 361 TIGISVARLFVLPILGIGIVVSADKLNFLVDGDPMYKFVLLLQYTTPTAILLGAIASLRG 420
           TIGISVARLFVLPILGIGIVVSADKLNFLVDGDPMYKFVLLLQYTTPTAILLGAIASLRG
Sbjct: 361 TIGISVARLFVLPILGIGIVVSADKLNFLVDGDPMYKFVLLLQYTTPTAILLGAIASLRG 420

Query: 421 YAVREASALLFWEHIFALLSLSLYLFVYFKIVI 454
           YAVREASALLFWEHIFALLSLSLYLFVYFKIVI
Sbjct: 421 YAVREASALLFWEHIFALLSLSLYLFVYFKIVI 453

BLAST of Moc04g27390 vs. NCBI nr
Match: XP_038875937.1 (protein PIN-LIKES 2 [Benincasa hispida])

HSP 1 Score: 782.7 bits (2020), Expect = 1.7e-222
Identity = 406/455 (89.23%), Postives = 433/455 (95.16%), Query Frame = 0

Query: 1   MGDYFSV-IDRSGVRSSSEDLLSAITPLMKLLSLTAIGLLLSHPKVQMIPRATLRLLSKL 60
           M DYFSV +DR+GVRSSSEDLL+AI PLMKLLSLTAIGLLLSHPK+QMIPR TLRLLSKL
Sbjct: 1   MADYFSVLVDRNGVRSSSEDLLTAIMPLMKLLSLTAIGLLLSHPKIQMIPRETLRLLSKL 60

Query: 61  VFALFLPCLIFTHLGESITLQNIAKWWFIPVNVLISTGIGCLLGFLVVILCRPPPRLMRF 120
           VFALFLPCLIFT+LGESITL+NIAKWWFIPVNVLISTGIGCLLGFLVVILCRPPP L+RF
Sbjct: 61  VFALFLPCLIFTYLGESITLENIAKWWFIPVNVLISTGIGCLLGFLVVILCRPPPPLVRF 120

Query: 121 TVISTAFGNTGNLPLAIVGSVCHTADNPFGQHCHSRGVSYVSFCQWVSVILAYTLVYHMM 180
           T+ISTAFGNTGNLPLAIV SVCHTADNPFG+HCHSRGVSYVSFCQWVSVILAYTLVYHMM
Sbjct: 121 TIISTAFGNTGNLPLAIVSSVCHTADNPFGKHCHSRGVSYVSFCQWVSVILAYTLVYHMM 180

Query: 181 EPPLEFYEIVEEGTEIEELEQISDNDASKPLLIEAEWPGIEEKETEHCKAPFIARIFKSI 240
           EPPLEFYEIVEEGTEIEEL + +DND SKPLL+EAEWPGIEEKETEHCKAPFIARIF SI
Sbjct: 181 EPPLEFYEIVEEGTEIEELVETNDNDVSKPLLMEAEWPGIEEKETEHCKAPFIARIFNSI 240

Query: 241 SNVSQSTFPDLDHSRE-SSTPTYPESIRCLAEPRVVRKIRIVAVQTPLKHILQPPTIASL 300
           SNVSQS+FPDLDHSR+ SSTPTYPESIRCLAEPRVVRKIRIVA QTP++HILQPPTIASL
Sbjct: 241 SNVSQSSFPDLDHSRDSSSTPTYPESIRCLAEPRVVRKIRIVAEQTPIQHILQPPTIASL 300

Query: 301 LAIIVGLVPQIRASFFGPDAPLSFISDSLEILAAAVVPFVMLMLGGMLAEGPHELSTLGL 360
           LAIIVGLVPQI+A FFG DAPL FIS+SLEI A A+VPFVML+LGGMLAEGP+E STLGL
Sbjct: 301 LAIIVGLVPQIKAFFFGADAPLLFISNSLEIFAGAMVPFVMLILGGMLAEGPNESSTLGL 360

Query: 361 RTTIGISVARLFVLPILGIGIVVSADKLNFLVDGDPMYKFVLLLQYTTPTAILLGAIASL 420
           RTTIGI +ARL VLP+LGIGIVV+ADKLNFLV GDPMYKFVLLLQYTTPTAILLGA+ASL
Sbjct: 361 RTTIGICIARLLVLPVLGIGIVVAADKLNFLVHGDPMYKFVLLLQYTTPTAILLGALASL 420

Query: 421 RGYAVREASALLFWEHIFALLSLSLYLFVYFKIVI 454
           RGYAVREASALLFWEH+FALLSLSLY+FVYFK VI
Sbjct: 421 RGYAVREASALLFWEHVFALLSLSLYIFVYFKAVI 455

BLAST of Moc04g27390 vs. NCBI nr
Match: XP_022939090.1 (protein PIN-LIKES 2 [Cucurbita moschata] >XP_022939092.1 protein PIN-LIKES 2 [Cucurbita moschata] >KAG7016758.1 Protein PIN-LIKES 2 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 782.3 bits (2019), Expect = 2.2e-222
Identity = 403/454 (88.77%), Postives = 432/454 (95.15%), Query Frame = 0

Query: 1   MGDYFSVIDRSGVRSSSEDLLSAITPLMKLLSLTAIGLLLSHPKVQMIPRATLRLLSKLV 60
           M DYFSV DR+ VRSSSEDLLSAI PLMKLLSLTAIGLLL+HPK+QMIPRATLRLLSKLV
Sbjct: 1   MADYFSVGDRNAVRSSSEDLLSAIMPLMKLLSLTAIGLLLAHPKIQMIPRATLRLLSKLV 60

Query: 61  FALFLPCLIFTHLGESITLQNIAKWWFIPVNVLISTGIGCLLGFLVVILCRPPPRLMRFT 120
           FALFLPCLIFT+LGESITL NIAKWWFIPVNV+ISTGIGC LGFLVVILCRPPP L+RFT
Sbjct: 61  FALFLPCLIFTYLGESITLDNIAKWWFIPVNVVISTGIGCFLGFLVVILCRPPPHLIRFT 120

Query: 121 VISTAFGNTGNLPLAIVGSVCHTADNPFGQHCHSRGVSYVSFCQWVSVILAYTLVYHMME 180
           +IST FGNTGNLPLAIV SVCHTADNPFG HCHSRGVSYVSFCQWVSVILAYTLVYHMME
Sbjct: 121 IISTGFGNTGNLPLAIVSSVCHTADNPFGPHCHSRGVSYVSFCQWVSVILAYTLVYHMME 180

Query: 181 PPLEFYEIVEEGTEIEELEQISDNDASKPLLIEAEWPGIEEKETEHCKAPFIARIFKSIS 240
           PP+EFYEIVEEGTEIEEL + SDND SKPLLIEAEWPGIEEKETE+CKAPFIARIFKSIS
Sbjct: 181 PPMEFYEIVEEGTEIEELVESSDNDVSKPLLIEAEWPGIEEKETENCKAPFIARIFKSIS 240

Query: 241 NVSQSTFPDLDHSRESS-TPTYPESIRCLAEPRVVRKIRIVAVQTPLKHILQPPTIASLL 300
           NVSQSTFPDLDHSR+SS TPTYPESIRCLAEPRVVRKIRIVA QTP++HILQPPTIASLL
Sbjct: 241 NVSQSTFPDLDHSRDSSTTPTYPESIRCLAEPRVVRKIRIVAEQTPIQHILQPPTIASLL 300

Query: 301 AIIVGLVPQIRASFFGPDAPLSFISDSLEILAAAVVPFVMLMLGGMLAEGPHELSTLGLR 360
           AIIVGLVPQI+A FFG +APL FIS+SLEILA A+VPFVML+LGGMLAEGP+E STLGLR
Sbjct: 301 AIIVGLVPQIKACFFGSEAPLLFISNSLEILAGAMVPFVMLILGGMLAEGPNESSTLGLR 360

Query: 361 TTIGISVARLFVLPILGIGIVVSADKLNFLVDGDPMYKFVLLLQYTTPTAILLGAIASLR 420
           TTIGIS+ARL VLP+LGIGI+V+ADKLNFLV GDPMYKFV+LLQYTTPTAIL+GAIASLR
Sbjct: 361 TTIGISIARLLVLPVLGIGIIVAADKLNFLVHGDPMYKFVVLLQYTTPTAILVGAIASLR 420

Query: 421 GYAVREASALLFWEHIFALLSLSLYLFVYFKIVI 454
           GYAVREASALLFWEH+FALLSLSLY+FVYFK+V+
Sbjct: 421 GYAVREASALLFWEHVFALLSLSLYIFVYFKVVL 454

BLAST of Moc04g27390 vs. NCBI nr
Match: KAG6579244.1 (Protein PIN-LIKES 2, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 782.3 bits (2019), Expect = 2.2e-222
Identity = 403/454 (88.77%), Postives = 432/454 (95.15%), Query Frame = 0

Query: 1   MGDYFSVIDRSGVRSSSEDLLSAITPLMKLLSLTAIGLLLSHPKVQMIPRATLRLLSKLV 60
           M DYFSV DR+ VRSSSEDLLSAI PLMKLLSLTAIGLLL+HPK+QMIPRATLRLLSKLV
Sbjct: 43  MADYFSVGDRNAVRSSSEDLLSAIMPLMKLLSLTAIGLLLAHPKIQMIPRATLRLLSKLV 102

Query: 61  FALFLPCLIFTHLGESITLQNIAKWWFIPVNVLISTGIGCLLGFLVVILCRPPPRLMRFT 120
           FALFLPCLIFT+LGESITL NIAKWWFIPVNV+ISTGIGC LGFLVVILCRPPP L+RFT
Sbjct: 103 FALFLPCLIFTYLGESITLDNIAKWWFIPVNVVISTGIGCFLGFLVVILCRPPPHLIRFT 162

Query: 121 VISTAFGNTGNLPLAIVGSVCHTADNPFGQHCHSRGVSYVSFCQWVSVILAYTLVYHMME 180
           +IST FGNTGNLPLAIV SVCHTADNPFG HCHSRGVSYVSFCQWVSVILAYTLVYHMME
Sbjct: 163 IISTGFGNTGNLPLAIVSSVCHTADNPFGPHCHSRGVSYVSFCQWVSVILAYTLVYHMME 222

Query: 181 PPLEFYEIVEEGTEIEELEQISDNDASKPLLIEAEWPGIEEKETEHCKAPFIARIFKSIS 240
           PP+EFYEIVEEGTEIEEL + SDND SKPLLIEAEWPGIEEKETE+CKAPFIARIFKSIS
Sbjct: 223 PPMEFYEIVEEGTEIEELVESSDNDVSKPLLIEAEWPGIEEKETENCKAPFIARIFKSIS 282

Query: 241 NVSQSTFPDLDHSRESS-TPTYPESIRCLAEPRVVRKIRIVAVQTPLKHILQPPTIASLL 300
           NVSQSTFPDLDHSR+SS TPTYPESIRCLAEPRVVRKIRIVA QTP++HILQPPTIASLL
Sbjct: 283 NVSQSTFPDLDHSRDSSTTPTYPESIRCLAEPRVVRKIRIVAEQTPIQHILQPPTIASLL 342

Query: 301 AIIVGLVPQIRASFFGPDAPLSFISDSLEILAAAVVPFVMLMLGGMLAEGPHELSTLGLR 360
           AIIVGLVPQI+A FFG +APL FIS+SLEILA A+VPFVML+LGGMLAEGP+E STLGLR
Sbjct: 343 AIIVGLVPQIKACFFGSEAPLLFISNSLEILAGAMVPFVMLILGGMLAEGPNESSTLGLR 402

Query: 361 TTIGISVARLFVLPILGIGIVVSADKLNFLVDGDPMYKFVLLLQYTTPTAILLGAIASLR 420
           TTIGIS+ARL VLP+LGIGI+V+ADKLNFLV GDPMYKFV+LLQYTTPTAIL+GAIASLR
Sbjct: 403 TTIGISIARLLVLPVLGIGIIVAADKLNFLVHGDPMYKFVVLLQYTTPTAILVGAIASLR 462

Query: 421 GYAVREASALLFWEHIFALLSLSLYLFVYFKIVI 454
           GYAVREASALLFWEH+FALLSLSLY+FVYFK+V+
Sbjct: 463 GYAVREASALLFWEHVFALLSLSLYIFVYFKVVL 496

BLAST of Moc04g27390 vs. NCBI nr
Match: XP_022993212.1 (protein PIN-LIKES 2 [Cucurbita maxima] >XP_022993213.1 protein PIN-LIKES 2 [Cucurbita maxima])

HSP 1 Score: 780.0 bits (2013), Expect = 1.1e-221
Identity = 402/454 (88.55%), Postives = 432/454 (95.15%), Query Frame = 0

Query: 1   MGDYFSVIDRSGVRSSSEDLLSAITPLMKLLSLTAIGLLLSHPKVQMIPRATLRLLSKLV 60
           M DYFSV D++ VRSSSEDLLSAI PLMKLLSLTAIGLLL+HPK+QMIPRATLRLLSKLV
Sbjct: 1   MADYFSVGDQNEVRSSSEDLLSAIMPLMKLLSLTAIGLLLAHPKIQMIPRATLRLLSKLV 60

Query: 61  FALFLPCLIFTHLGESITLQNIAKWWFIPVNVLISTGIGCLLGFLVVILCRPPPRLMRFT 120
           FALFLPCLIFT+LGESITL NIAKWWFIPVNV+ISTGIGC LGFLVVILCRPPP L+RFT
Sbjct: 61  FALFLPCLIFTYLGESITLDNIAKWWFIPVNVVISTGIGCFLGFLVVILCRPPPHLIRFT 120

Query: 121 VISTAFGNTGNLPLAIVGSVCHTADNPFGQHCHSRGVSYVSFCQWVSVILAYTLVYHMME 180
           +IST FGNTGNLPLAIV SVCHTADNPFG HCHSRGVSYVSFCQWVSVILAYTLVYHMME
Sbjct: 121 IISTGFGNTGNLPLAIVSSVCHTADNPFGPHCHSRGVSYVSFCQWVSVILAYTLVYHMME 180

Query: 181 PPLEFYEIVEEGTEIEELEQISDNDASKPLLIEAEWPGIEEKETEHCKAPFIARIFKSIS 240
           PP+EFYEIVEEGTEIEEL + SDND SKPLLIEAEWPGIEEKETE+CKAPFIARIFKSIS
Sbjct: 181 PPMEFYEIVEEGTEIEELVESSDNDVSKPLLIEAEWPGIEEKETENCKAPFIARIFKSIS 240

Query: 241 NVSQSTFPDLDHSRESS-TPTYPESIRCLAEPRVVRKIRIVAVQTPLKHILQPPTIASLL 300
           NVSQSTFPDLDHSR+SS TPTYPESIRCLAEPRVVRKIRIVA QTP++HILQPPTIASLL
Sbjct: 241 NVSQSTFPDLDHSRDSSTTPTYPESIRCLAEPRVVRKIRIVAEQTPIQHILQPPTIASLL 300

Query: 301 AIIVGLVPQIRASFFGPDAPLSFISDSLEILAAAVVPFVMLMLGGMLAEGPHELSTLGLR 360
           AIIVGLVPQI+A FFG +APL FIS+SLEILA A+VPFVML+LGGMLAEGP+E STLGLR
Sbjct: 301 AIIVGLVPQIKACFFGSEAPLLFISNSLEILAGAMVPFVMLILGGMLAEGPNESSTLGLR 360

Query: 361 TTIGISVARLFVLPILGIGIVVSADKLNFLVDGDPMYKFVLLLQYTTPTAILLGAIASLR 420
           TTIGIS+ARL VLP+LGIGI+V+ADKLNFLV GDPMYKFV+LLQYTTPTAIL+GAIASLR
Sbjct: 361 TTIGISIARLLVLPVLGIGIIVAADKLNFLVHGDPMYKFVVLLQYTTPTAILVGAIASLR 420

Query: 421 GYAVREASALLFWEHIFALLSLSLYLFVYFKIVI 454
           GYAVREASALLFWEH+FALLSLSLY+FVYFK+V+
Sbjct: 421 GYAVREASALLFWEHVFALLSLSLYIFVYFKVVL 454

BLAST of Moc04g27390 vs. ExPASy Swiss-Prot
Match: Q9C999 (Protein PIN-LIKES 2 OS=Arabidopsis thaliana OX=3702 GN=PILS2 PE=2 SV=1)

HSP 1 Score: 615.1 bits (1585), Expect = 6.1e-175
Identity = 314/443 (70.88%), Postives = 380/443 (85.78%), Query Frame = 0

Query: 13  VRSSSEDLLSAITPLMKLLSLTAIGLLLSHPKVQMIPRATLRLLSKLVFALFLPCLIFTH 72
           V S   D+LS + PL+KL+ LT IGLLL+HPK Q++PRAT RLLSKLVFALFLPCLIFT 
Sbjct: 9   VNSRVVDILSGVVPLLKLICLTVIGLLLAHPKTQLVPRATFRLLSKLVFALFLPCLIFTE 68

Query: 73  LGESITLQNIAKWWFIPVNVLISTGIGCLLGFLVVILCRPPPRLMRFTVISTAFGNTGNL 132
           LGESITL NI +WWFIPVNVL+S  +G L+G+LVV++CRPPP   RFT++ TAFGNTGNL
Sbjct: 69  LGESITLDNIVQWWFIPVNVLLSAVVGSLIGYLVVLICRPPPEFNRFTIVMTAFGNTGNL 128

Query: 133 PLAIVGSVCHTADNPFGQHCHSRGVSYVSFCQWVSVILAYTLVYHMMEPPLEFYEIV-EE 192
            LAIV SVCHT  NPFG +C+SRGVSYVSF QWV+VIL YT+VYHMMEPPLE+YE+V EE
Sbjct: 129 LLAIVSSVCHTKTNPFGPNCNSRGVSYVSFAQWVAVILVYTVVYHMMEPPLEYYEVVEEE 188

Query: 193 GTEIEELEQISDNDASKPLLIEAEWPGIEEKETEHCKAPFIARIFKSISNVSQSTFPDLD 252
           G EIEE+  + ++DAS+PLL+EAEWPGIE+KETEHCK PFIAR+F SIS+ SQ++FP++D
Sbjct: 189 GVEIEEI-NVENHDASRPLLVEAEWPGIEDKETEHCKTPFIARVFNSISSFSQTSFPEVD 248

Query: 253 HSRE-SSTPTYPESIRCLAEPRVVRKIRIVAVQTPLKHILQPPTIASLLAIIVGLVPQIR 312
              E     + P SI+CLAEPRV+R+IR+VA QTP+KHILQPPTIASLLAII+G VPQ++
Sbjct: 249 LGGEYGGESSSPRSIQCLAEPRVMRRIRVVAEQTPVKHILQPPTIASLLAIIIGSVPQLK 308

Query: 313 ASFFGPDAPLSFISDSLEILAAAVVPFVMLMLGGMLAEGPHELSTLGLRTTIGISVARLF 372
           +  FG DAPLSFI+DSL I+ +A+VP VML+LGGML+EGP+E STLGLRTTIGISVARL 
Sbjct: 309 SVVFGYDAPLSFITDSLNIMGSAMVPSVMLVLGGMLSEGPNE-STLGLRTTIGISVARLL 368

Query: 373 VLPILGIGIVVSADKLNFLVDGDPMYKFVLLLQYTTPTAILLGAIASLRGYAVREASALL 432
           VLP++GIGIV+SADKL  +   DPM+KFVLLLQY+TP+AILLGAIASLRGYAVREASALL
Sbjct: 369 VLPLVGIGIVMSADKLGLISSADPMFKFVLLLQYSTPSAILLGAIASLRGYAVREASALL 428

Query: 433 FWEHIFALLSLSLYLFVYFKIVI 454
           FW+HIFALLSL+ Y+ ++FK+ +
Sbjct: 429 FWQHIFALLSLTFYIVIFFKLTV 449

BLAST of Moc04g27390 vs. ExPASy Swiss-Prot
Match: Q9LZN2 (Protein PIN-LIKES 6 OS=Arabidopsis thaliana OX=3702 GN=PILS6 PE=2 SV=1)

HSP 1 Score: 275.4 bits (703), Expect = 1.1e-72
Identity = 165/432 (38.19%), Postives = 252/432 (58.33%), Query Frame = 0

Query: 23  AITPLMKLLSLTAIGLLLSHPKVQMIPRATLRLLSKLVFALFLPCLIFTHLGESITLQNI 82
           A+ P+ K+ ++  +GLL++   V ++P +  +LL+ LVF+L LPCLIF+ LG+++TLQ +
Sbjct: 31  AVMPIAKVFTMCFLGLLMASKYVNILPPSGRKLLNGLVFSLLLPCLIFSQLGQAVTLQKM 90

Query: 83  AKWWFIPVNVLISTGIGCLLGFLVVILCRPPPRLMRFTVISTAFGNTGNLPLAIVGSVCH 142
            +WWFIPVNV++ T  G ++GF+V  + RPP    +FT+I    GN GN+PL ++ ++C 
Sbjct: 91  LQWWFIPVNVVLGTISGSIIGFIVASIVRPPYPYFKFTIIQIGVGNIGNVPLVLLAALCR 150

Query: 143 TADNPFG--QHCHSRGVSYVSFCQWVSVILAYTLVYHMMEPPLEFYEIVEEGTEIEELEQ 202
              NPFG  + C   G +Y+SF QWV  I+ YT VY M  PP E ++  EE         
Sbjct: 151 DTSNPFGDSEKCSIDGTAYISFGQWVGAIILYTYVYQMFAPPPEGFDAEEE--------- 210

Query: 203 ISDNDASKPLLIEAEWPGIEEKETEHCKAPFIARIFKSISNVSQSTFPDLDHSRESSTPT 262
              N A K L ++A             + P + + F    + +Q   P           T
Sbjct: 211 ---NLALKTLPVDAAPE----------QVPLLTQNFPKDFSPTQDLLP--------VQST 270

Query: 263 YPESIRCLAEPRVVRKIRIVAVQTPLKHILQPPTIASLLAIIVGLVPQIRASFFGPDAPL 322
            P       + ++ +    +  +  LK I+QP  +AS+LA+I+G +P  +   F   APL
Sbjct: 271 EPRGRGVSRKGKIAQIFVFLYEKLKLKQIVQPAIVASILAMILGAIPFTKKLIFTNGAPL 330

Query: 323 SFISDSLEILAAAVVPFVMLMLGGMLAEGPHELSTLGLRTTIGISVARLFVLPILGIGIV 382
            F +DS  IL  A++P ++L LGG L  GP   S LG +TT  I + RL ++P +G+GIV
Sbjct: 331 FFFTDSCMILGDAMIPCILLALGGNLINGPGS-SKLGFKTTAAIIIGRLVLVPPVGLGIV 390

Query: 383 VSADKLNFLVDGDPMYKFVLLLQYTTPTAILLGAIASLRGYAVREASALLFWEHIFALLS 442
             ADKL FL   D M++FVLLLQ+T PT++L GA+A+LRG   RE++A+LFW HIFA+ S
Sbjct: 391 TVADKLGFLPADDKMFRFVLLLQHTMPTSVLSGAVANLRGCG-RESAAVLFWVHIFAIFS 430

Query: 443 LSLYLFVYFKIV 453
           ++ ++ +Y  I+
Sbjct: 451 MAGWMVLYINIL 430

BLAST of Moc04g27390 vs. ExPASy Swiss-Prot
Match: Q9C9K5 (Protein PIN-LIKES 3 OS=Arabidopsis thaliana OX=3702 GN=PILS3 PE=2 SV=1)

HSP 1 Score: 183.0 bits (463), Expect = 7.8e-45
Identity = 125/429 (29.14%), Postives = 224/429 (52.21%), Query Frame = 0

Query: 18  EDLLSAITPLMKLLSLTAIGLLLSHPKVQMIPRATLRLLSKLVFALFLPCLIFTHLGESI 77
           E  +++  P++++L +T++G  ++   V ++     + L+ +VF +F P LI + L +S+
Sbjct: 6   ELFITSSKPVVEILLITSVGFYMALDGVNLLGHDARKYLNNIVFYVFSPSLIGSRLADSV 65

Query: 78  TLQNIAKWWFIPVNVLISTGIGCLLGFLVVILCRPPPRLMRFTVISTAFGNTGNLPLAIV 137
           T +++ K WF+PVNVL++  IG LLG++V+++ +PP  L    +   A GN GN+PL I+
Sbjct: 66  TYESLVKMWFMPVNVLLTFIIGSLLGWIVIVITKPPSHLRGLILGCCAAGNLGNMPLIII 125

Query: 138 GSVCHTADNPFG--QHCHSRGVSYVSFCQWVSVILAYTLVYHMMEPPLEFYEIVEEGTEI 197
            +VC     PFG  + C   G+ YV+    +  I  +T VY++M                
Sbjct: 126 PAVCKEKGGPFGDPESCQKYGMGYVALSMAMGSIYIWTYVYNLM---------------- 185

Query: 198 EELEQISDNDASKPLLIEAEWPGIEEKETEHCKAPFIARIFKSISNVSQSTFPDLDHSRE 257
                +S++    P  +E+ +        +  K P I+                   S+E
Sbjct: 186 ---RVLSNSPVETPPSVESNY--------DSYKVPLIS-------------------SKE 245

Query: 258 SSTPTYPESIRCLAEPRVVRKIRIVAVQTPLKHILQPPTIASLLAIIVGLVPQIRASFFG 317
                   + +     +V R++  ++ +  LK I  P TIA+++A+++GL+  +R    G
Sbjct: 246 EE-----NNQKAGRWEKVKRRLVSLSQKVNLKTIFAPSTIAAMIALVIGLITPLRKLIIG 305

Query: 318 PDAPLSFISDSLEILAAAVVPFVMLMLGGMLAEGPHELSTLGLRTTIGISVARLFVLPIL 377
            +APL  + DS+ ++    VP + +++GG L +G    S + + + IG+ VAR  +LP+ 
Sbjct: 306 TEAPLRVLQDSVTLVGDGAVPAMTMIIGGNLLKGLRS-SGMKMSSIIGVLVARYVLLPMS 365

Query: 378 GIGIVVSADKLNFLVDGDPMYKFVLLLQYTTPTAILLGAIASLRGYAVREASALLFWEHI 437
           G+ IV  A KL+ LV  +P+Y+FVLLLQY  P A+ LG I  L G    E S ++ W + 
Sbjct: 366 GVLIVRGAYKLD-LVTSEPLYQFVLLLQYAVPPAMNLGTITQLFGTGESECSVIMLWTYS 381

Query: 438 FALLSLSLY 445
            A ++L+++
Sbjct: 426 LASIALTVW 381

BLAST of Moc04g27390 vs. ExPASy Swiss-Prot
Match: Q9SHL8 (Protein PIN-LIKES 5 OS=Arabidopsis thaliana OX=3702 GN=PILS5 PE=2 SV=1)

HSP 1 Score: 182.2 bits (461), Expect = 1.3e-44
Identity = 125/434 (28.80%), Postives = 223/434 (51.38%), Query Frame = 0

Query: 23  AITPLMKLLSLTAIGLLLSHPKVQMIPRATLRLLSKLVFALFLPCLIFTHLGESITLQNI 82
           A  P++++L ++ +G  ++  + ++ P      ++K+VF LF P L+F +L +++TL++I
Sbjct: 10  ASMPVIQVLFMSLVGAFMASDRCKLFPVEARNSMNKVVFVLFAPALMFANLAQTVTLEDI 69

Query: 83  AKWWFIPVNVLISTGIGCLLGFLVVILCRPPPRLMRFTVISTAFGNTGNLPLAIVGSVCH 142
             WWF+PVN+ ++  IG LLG+LVV + +PPP L    V + + GN GNLP+ +V ++C 
Sbjct: 70  ISWWFMPVNMGLTFLIGGLLGWLVVKILKPPPYLEGLIVATCSAGNMGNLPIILVPAICD 129

Query: 143 TADNPFGQH--CHSRGVSYVSFCQWVSVILAYTLVYHMMEPPLEFYEIVEEGTEIEELEQ 202
              +PFG    C + G+SY SF   +     +T  + +++      + +EE  +I     
Sbjct: 130 EDKSPFGNRSVCRTVGLSYASFSMALGGFYIWTYTFRLIKGSAMKVQAIEESEKIAIKSS 189

Query: 203 ISDNDAS-KPLLIEAEWPGIEEKETEHCKAPFIARIFKSISNVSQSTFPDLDHSRESSTP 262
            SD +A  K  L+ A     E+KE +  K                             T 
Sbjct: 190 NSDLEADHKTHLLGAP----EDKENKVVK---------------------------EKTG 249

Query: 263 TYPESIRCLAEPRVVRKIRIVAVQTPLKHILQPPTIASLLAIIVGLVPQIRASFFGPDAP 322
            + + +  L E               L+ +L PPT+ +++  I G V  +R    G DAP
Sbjct: 250 FWRKGVDFLHE--------------ILEELLAPPTLGAIIGFIFGAVRWLRNLIIGDDAP 309

Query: 323 LSFISDSLEILAAAVVPFVMLMLGGMLAEGPHELSTLGLRTTIGISVARLFVLPILGIGI 382
           L  +  + ++L    +P + ++LGG L +G    S +     +GI   R   +PI+GIGI
Sbjct: 310 LRIVQSTAKLLGDGTIPCMTIILGGNLIQGLRS-SAVKPMVVLGIVCVRYIAMPIIGIGI 369

Query: 383 VVSADKLNFLVDGDPMYKFVLLLQYTTPTAILLGAIASLRGYAVREASALLFWEHIFALL 442
           V++A  L FL   DP++++VL+LQ+T P A+ +G +  L   A  E S L+ W ++ A+L
Sbjct: 370 VLTAANLGFL-PADPLFQYVLMLQFTLPPAMNIGTMTQLYNVAQDECSVLMLWTYLVAIL 396

Query: 443 SLSLYLFVYFKIVI 454
           +L+++  ++  +++
Sbjct: 430 ALTVWSTIFLHLLV 396

BLAST of Moc04g27390 vs. ExPASy Swiss-Prot
Match: F4HWB6 (Protein PIN-LIKES 1 OS=Arabidopsis thaliana OX=3702 GN=PILS1 PE=2 SV=1)

HSP 1 Score: 180.3 bits (456), Expect = 5.0e-44
Identity = 130/426 (30.52%), Postives = 213/426 (50.00%), Query Frame = 0

Query: 21  LSAITPLMKLLSLTAIGLLLSHPKVQMIPRATLRLLSKLVFALFLPCLIFTHLGESITLQ 80
           +++  P+ K+L +T IG  L+  +V ++     + L+ +VF +F P L+ + L E+IT +
Sbjct: 96  ITSSIPVAKILLITGIGFYLALDQVNILNHDARKQLNNIVFYVFSPSLVASSLSETITYE 155

Query: 81  NIAKWWFIPVNVLISTGIGCLLGFLVVILCRPPPRLMRFTVISTAFGNTGNLPLAIVGSV 140
           ++ K WF+P+NVL++  IG  LG++V+ + +PP  L    V   A GN GN+PL I+ ++
Sbjct: 156 SMVKMWFMPLNVLLTFIIGSFLGWIVIKITKPPSHLRGIIVGCCAAGNLGNMPLIIIPAI 215

Query: 141 CHTADNPFG--QHCHSRGVSYVSFCQWVSVILAYTLVYHMMEPPLEFYEIVEEGTEIEEL 200
           C+   +PFG  + C   G+ Y++    +  I  +T VY++M                   
Sbjct: 216 CNEKGSPFGDPESCEKFGLGYIALSMAIGAIYIWTYVYNLM------------------- 275

Query: 201 EQISDNDASKPLLIEAEWPGIEEKETEHCKAPFIARIFKSISNVSQSTFPDLDHSRESST 260
            ++  N A +  +                             N + ST P +       +
Sbjct: 276 -RMLANPAGETAI-----------------------------NSTSSTMPLI-------S 335

Query: 261 PTYPESIRCLAEPRVVRKIRIVAVQTPLKHILQPPTIASLLAIIVGLVPQIRASFFGPDA 320
           P    + +     +V +++  VA +  L+ I  P TIA+L+A+ VGL P +R    G  A
Sbjct: 336 PKVEVAEQVGTWGKVKQRVCSVAEKINLRTIFAPSTIAALIALAVGLNPLLRKLLVGNTA 395

Query: 321 PLSFISDSLEILAAAVVPFVMLMLGGMLAEGPHELSTLGLRTTIGISVARLFVLPILGIG 380
           PL  I DS+ +L    +P + L++GG L  G    S +     +G+ V R  +LPILG+ 
Sbjct: 396 PLRVIEDSVSLLGDGAIPVLTLIVGGNLLNGLRG-SGINKSVIMGVVVVRYLLLPILGVF 455

Query: 381 IVVSADKLNFLVDGDPMYKFVLLLQYTTPTAILLGAIASLRGYAVREASALLFWEHIFAL 440
           IV  A  L  LV  +P+Y+FVLLLQY  P A+ LG I  L G    E S +LFW +  A 
Sbjct: 456 IVRGAHYLG-LVTSEPLYQFVLLLQYVVPPAMNLGTITQLFGSGESECSVILFWSYALAS 463

Query: 441 LSLSLY 445
           +SL+++
Sbjct: 516 VSLTVW 463

BLAST of Moc04g27390 vs. ExPASy TrEMBL
Match: A0A6J1DDP0 (protein PIN-LIKES 2-like OS=Momordica charantia OX=3673 GN=LOC111020086 PE=4 SV=1)

HSP 1 Score: 866.7 bits (2238), Expect = 4.3e-248
Identity = 453/453 (100.00%), Postives = 453/453 (100.00%), Query Frame = 0

Query: 1   MGDYFSVIDRSGVRSSSEDLLSAITPLMKLLSLTAIGLLLSHPKVQMIPRATLRLLSKLV 60
           MGDYFSVIDRSGVRSSSEDLLSAITPLMKLLSLTAIGLLLSHPKVQMIPRATLRLLSKLV
Sbjct: 1   MGDYFSVIDRSGVRSSSEDLLSAITPLMKLLSLTAIGLLLSHPKVQMIPRATLRLLSKLV 60

Query: 61  FALFLPCLIFTHLGESITLQNIAKWWFIPVNVLISTGIGCLLGFLVVILCRPPPRLMRFT 120
           FALFLPCLIFTHLGESITLQNIAKWWFIPVNVLISTGIGCLLGFLVVILCRPPPRLMRFT
Sbjct: 61  FALFLPCLIFTHLGESITLQNIAKWWFIPVNVLISTGIGCLLGFLVVILCRPPPRLMRFT 120

Query: 121 VISTAFGNTGNLPLAIVGSVCHTADNPFGQHCHSRGVSYVSFCQWVSVILAYTLVYHMME 180
           VISTAFGNTGNLPLAIVGSVCHTADNPFGQHCHSRGVSYVSFCQWVSVILAYTLVYHMME
Sbjct: 121 VISTAFGNTGNLPLAIVGSVCHTADNPFGQHCHSRGVSYVSFCQWVSVILAYTLVYHMME 180

Query: 181 PPLEFYEIVEEGTEIEELEQISDNDASKPLLIEAEWPGIEEKETEHCKAPFIARIFKSIS 240
           PPLEFYEIVEEGTEIEELEQISDNDASKPLLIEAEWPGIEEKETEHCKAPFIARIFKSIS
Sbjct: 181 PPLEFYEIVEEGTEIEELEQISDNDASKPLLIEAEWPGIEEKETEHCKAPFIARIFKSIS 240

Query: 241 NVSQSTFPDLDHSRESSTPTYPESIRCLAEPRVVRKIRIVAVQTPLKHILQPPTIASLLA 300
           NVSQSTFPDLDHSRESSTPTYPESIRCLAEPRVVRKIRIVAVQTPLKHILQPPTIASLLA
Sbjct: 241 NVSQSTFPDLDHSRESSTPTYPESIRCLAEPRVVRKIRIVAVQTPLKHILQPPTIASLLA 300

Query: 301 IIVGLVPQIRASFFGPDAPLSFISDSLEILAAAVVPFVMLMLGGMLAEGPHELSTLGLRT 360
           IIVGLVPQIRASFFGPDAPLSFISDSLEILAAAVVPFVMLMLGGMLAEGPHELSTLGLRT
Sbjct: 301 IIVGLVPQIRASFFGPDAPLSFISDSLEILAAAVVPFVMLMLGGMLAEGPHELSTLGLRT 360

Query: 361 TIGISVARLFVLPILGIGIVVSADKLNFLVDGDPMYKFVLLLQYTTPTAILLGAIASLRG 420
           TIGISVARLFVLPILGIGIVVSADKLNFLVDGDPMYKFVLLLQYTTPTAILLGAIASLRG
Sbjct: 361 TIGISVARLFVLPILGIGIVVSADKLNFLVDGDPMYKFVLLLQYTTPTAILLGAIASLRG 420

Query: 421 YAVREASALLFWEHIFALLSLSLYLFVYFKIVI 454
           YAVREASALLFWEHIFALLSLSLYLFVYFKIVI
Sbjct: 421 YAVREASALLFWEHIFALLSLSLYLFVYFKIVI 453

BLAST of Moc04g27390 vs. ExPASy TrEMBL
Match: A0A6J1FFU8 (protein PIN-LIKES 2 OS=Cucurbita moschata OX=3662 GN=LOC111445095 PE=4 SV=1)

HSP 1 Score: 782.3 bits (2019), Expect = 1.1e-222
Identity = 403/454 (88.77%), Postives = 432/454 (95.15%), Query Frame = 0

Query: 1   MGDYFSVIDRSGVRSSSEDLLSAITPLMKLLSLTAIGLLLSHPKVQMIPRATLRLLSKLV 60
           M DYFSV DR+ VRSSSEDLLSAI PLMKLLSLTAIGLLL+HPK+QMIPRATLRLLSKLV
Sbjct: 1   MADYFSVGDRNAVRSSSEDLLSAIMPLMKLLSLTAIGLLLAHPKIQMIPRATLRLLSKLV 60

Query: 61  FALFLPCLIFTHLGESITLQNIAKWWFIPVNVLISTGIGCLLGFLVVILCRPPPRLMRFT 120
           FALFLPCLIFT+LGESITL NIAKWWFIPVNV+ISTGIGC LGFLVVILCRPPP L+RFT
Sbjct: 61  FALFLPCLIFTYLGESITLDNIAKWWFIPVNVVISTGIGCFLGFLVVILCRPPPHLIRFT 120

Query: 121 VISTAFGNTGNLPLAIVGSVCHTADNPFGQHCHSRGVSYVSFCQWVSVILAYTLVYHMME 180
           +IST FGNTGNLPLAIV SVCHTADNPFG HCHSRGVSYVSFCQWVSVILAYTLVYHMME
Sbjct: 121 IISTGFGNTGNLPLAIVSSVCHTADNPFGPHCHSRGVSYVSFCQWVSVILAYTLVYHMME 180

Query: 181 PPLEFYEIVEEGTEIEELEQISDNDASKPLLIEAEWPGIEEKETEHCKAPFIARIFKSIS 240
           PP+EFYEIVEEGTEIEEL + SDND SKPLLIEAEWPGIEEKETE+CKAPFIARIFKSIS
Sbjct: 181 PPMEFYEIVEEGTEIEELVESSDNDVSKPLLIEAEWPGIEEKETENCKAPFIARIFKSIS 240

Query: 241 NVSQSTFPDLDHSRESS-TPTYPESIRCLAEPRVVRKIRIVAVQTPLKHILQPPTIASLL 300
           NVSQSTFPDLDHSR+SS TPTYPESIRCLAEPRVVRKIRIVA QTP++HILQPPTIASLL
Sbjct: 241 NVSQSTFPDLDHSRDSSTTPTYPESIRCLAEPRVVRKIRIVAEQTPIQHILQPPTIASLL 300

Query: 301 AIIVGLVPQIRASFFGPDAPLSFISDSLEILAAAVVPFVMLMLGGMLAEGPHELSTLGLR 360
           AIIVGLVPQI+A FFG +APL FIS+SLEILA A+VPFVML+LGGMLAEGP+E STLGLR
Sbjct: 301 AIIVGLVPQIKACFFGSEAPLLFISNSLEILAGAMVPFVMLILGGMLAEGPNESSTLGLR 360

Query: 361 TTIGISVARLFVLPILGIGIVVSADKLNFLVDGDPMYKFVLLLQYTTPTAILLGAIASLR 420
           TTIGIS+ARL VLP+LGIGI+V+ADKLNFLV GDPMYKFV+LLQYTTPTAIL+GAIASLR
Sbjct: 361 TTIGISIARLLVLPVLGIGIIVAADKLNFLVHGDPMYKFVVLLQYTTPTAILVGAIASLR 420

Query: 421 GYAVREASALLFWEHIFALLSLSLYLFVYFKIVI 454
           GYAVREASALLFWEH+FALLSLSLY+FVYFK+V+
Sbjct: 421 GYAVREASALLFWEHVFALLSLSLYIFVYFKVVL 454

BLAST of Moc04g27390 vs. ExPASy TrEMBL
Match: A0A6J1JS57 (protein PIN-LIKES 2 OS=Cucurbita maxima OX=3661 GN=LOC111489301 PE=4 SV=1)

HSP 1 Score: 780.0 bits (2013), Expect = 5.3e-222
Identity = 402/454 (88.55%), Postives = 432/454 (95.15%), Query Frame = 0

Query: 1   MGDYFSVIDRSGVRSSSEDLLSAITPLMKLLSLTAIGLLLSHPKVQMIPRATLRLLSKLV 60
           M DYFSV D++ VRSSSEDLLSAI PLMKLLSLTAIGLLL+HPK+QMIPRATLRLLSKLV
Sbjct: 1   MADYFSVGDQNEVRSSSEDLLSAIMPLMKLLSLTAIGLLLAHPKIQMIPRATLRLLSKLV 60

Query: 61  FALFLPCLIFTHLGESITLQNIAKWWFIPVNVLISTGIGCLLGFLVVILCRPPPRLMRFT 120
           FALFLPCLIFT+LGESITL NIAKWWFIPVNV+ISTGIGC LGFLVVILCRPPP L+RFT
Sbjct: 61  FALFLPCLIFTYLGESITLDNIAKWWFIPVNVVISTGIGCFLGFLVVILCRPPPHLIRFT 120

Query: 121 VISTAFGNTGNLPLAIVGSVCHTADNPFGQHCHSRGVSYVSFCQWVSVILAYTLVYHMME 180
           +IST FGNTGNLPLAIV SVCHTADNPFG HCHSRGVSYVSFCQWVSVILAYTLVYHMME
Sbjct: 121 IISTGFGNTGNLPLAIVSSVCHTADNPFGPHCHSRGVSYVSFCQWVSVILAYTLVYHMME 180

Query: 181 PPLEFYEIVEEGTEIEELEQISDNDASKPLLIEAEWPGIEEKETEHCKAPFIARIFKSIS 240
           PP+EFYEIVEEGTEIEEL + SDND SKPLLIEAEWPGIEEKETE+CKAPFIARIFKSIS
Sbjct: 181 PPMEFYEIVEEGTEIEELVESSDNDVSKPLLIEAEWPGIEEKETENCKAPFIARIFKSIS 240

Query: 241 NVSQSTFPDLDHSRESS-TPTYPESIRCLAEPRVVRKIRIVAVQTPLKHILQPPTIASLL 300
           NVSQSTFPDLDHSR+SS TPTYPESIRCLAEPRVVRKIRIVA QTP++HILQPPTIASLL
Sbjct: 241 NVSQSTFPDLDHSRDSSTTPTYPESIRCLAEPRVVRKIRIVAEQTPIQHILQPPTIASLL 300

Query: 301 AIIVGLVPQIRASFFGPDAPLSFISDSLEILAAAVVPFVMLMLGGMLAEGPHELSTLGLR 360
           AIIVGLVPQI+A FFG +APL FIS+SLEILA A+VPFVML+LGGMLAEGP+E STLGLR
Sbjct: 301 AIIVGLVPQIKACFFGSEAPLLFISNSLEILAGAMVPFVMLILGGMLAEGPNESSTLGLR 360

Query: 361 TTIGISVARLFVLPILGIGIVVSADKLNFLVDGDPMYKFVLLLQYTTPTAILLGAIASLR 420
           TTIGIS+ARL VLP+LGIGI+V+ADKLNFLV GDPMYKFV+LLQYTTPTAIL+GAIASLR
Sbjct: 361 TTIGISIARLLVLPVLGIGIIVAADKLNFLVHGDPMYKFVVLLQYTTPTAILVGAIASLR 420

Query: 421 GYAVREASALLFWEHIFALLSLSLYLFVYFKIVI 454
           GYAVREASALLFWEH+FALLSLSLY+FVYFK+V+
Sbjct: 421 GYAVREASALLFWEHVFALLSLSLYIFVYFKVVL 454

BLAST of Moc04g27390 vs. ExPASy TrEMBL
Match: A0A0A0KRM3 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G571470 PE=4 SV=1)

HSP 1 Score: 753.8 bits (1945), Expect = 4.1e-214
Identity = 390/454 (85.90%), Postives = 423/454 (93.17%), Query Frame = 0

Query: 1   MGDYFS-VIDRSGVRSSSEDLLSAITPLMKLLSLTAIGLLLSHPKVQMIPRATLRLLSKL 60
           M DYFS V+DR+ V SS+ DLL+AI PLMKLLSLTAIGLLLSHPK+QMIPRATLRL+SKL
Sbjct: 1   MDDYFSVVVDRNEVGSSTGDLLTAIVPLMKLLSLTAIGLLLSHPKIQMIPRATLRLISKL 60

Query: 61  VFALFLPCLIFTHLGESITLQNIAKWWFIPVNVLISTGIGCLLGFLVVILCRPPPRLMRF 120
           VFALFLPCLIFTHLGE+ITL+NIAKWWF+PVNVLISTGIGCLLGFLVVI+CRPPP+L+RF
Sbjct: 61  VFALFLPCLIFTHLGENITLENIAKWWFVPVNVLISTGIGCLLGFLVVIICRPPPQLVRF 120

Query: 121 TVISTAFGNTGNLPLAIVGSVCHTADNPFGQHCHSRGVSYVSFCQWVSVILAYTLVYHMM 180
           T+ISTAFGNTGNLPLAIV SVCHT DNPFG++CHS+GVSYVSFCQWVSVI+AYTLVYHMM
Sbjct: 121 TIISTAFGNTGNLPLAIVSSVCHTDDNPFGKNCHSKGVSYVSFCQWVSVIIAYTLVYHMM 180

Query: 181 EPPLEFYEIVEEGTEIEELEQISDNDASKPLLIEAEWPGIEEKETEHCKAPFIARIFKSI 240
           EPPLEFYEIVEEGTEIEEL +  DND SKPLL+EAEWPGIEEKETEHCK PFIAR+F SI
Sbjct: 181 EPPLEFYEIVEEGTEIEELVESDDNDVSKPLLMEAEWPGIEEKETEHCKVPFIARVFNSI 240

Query: 241 SNVSQSTFPDLDHSRESSTPTYPESIRCLAEPRVVRKIRIVAVQTPLKHILQPPTIASLL 300
           SNVSQSTFPDLDH R+SST  YPESI CLAEPRVVRKIRIVA QTP++HILQPPTIASLL
Sbjct: 241 SNVSQSTFPDLDH-RDSSTSAYPESITCLAEPRVVRKIRIVAEQTPIQHILQPPTIASLL 300

Query: 301 AIIVGLVPQIRASFFGPDAPLSFISDSLEILAAAVVPFVMLMLGGMLAEGPHELSTLGLR 360
           AIIVGLVPQI+A  FG DAPL FIS+S EI A A+VPFVML+LGGMLAEGP+E STLGLR
Sbjct: 301 AIIVGLVPQIKAVLFGADAPLLFISNSFEIFAGAMVPFVMLILGGMLAEGPNESSTLGLR 360

Query: 361 TTIGISVARLFVLPILGIGIVVSADKLNFLVDGDPMYKFVLLLQYTTPTAILLGAIASLR 420
           TTIGI VARL VLP+LGIGIVV+ADKLNFLV GDPMYKFVLLLQYTTPTAILLGA+ASLR
Sbjct: 361 TTIGICVARLLVLPVLGIGIVVAADKLNFLVHGDPMYKFVLLLQYTTPTAILLGALASLR 420

Query: 421 GYAVREASALLFWEHIFALLSLSLYLFVYFKIVI 454
           GYAV+EASALLFWEHIFALLSLSLY+FVYFK VI
Sbjct: 421 GYAVKEASALLFWEHIFALLSLSLYVFVYFKAVI 453

BLAST of Moc04g27390 vs. ExPASy TrEMBL
Match: A0A5D3E4I5 (Protein PIN-LIKES 2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold426G00240 PE=4 SV=1)

HSP 1 Score: 753.4 bits (1944), Expect = 5.3e-214
Identity = 389/456 (85.31%), Postives = 424/456 (92.98%), Query Frame = 0

Query: 1   MGDYFS-VIDRSGVRSSSEDLLSAITPLMKLLSLTAIGLLLSHPKVQMIPRATLRLLSKL 60
           M DY S V+D + VRS+S DLLSAI PLMKLLSLTAIGLLLSHPK+QMIPRATLRLLSKL
Sbjct: 1   MDDYLSVVVDPNEVRSTSGDLLSAIVPLMKLLSLTAIGLLLSHPKIQMIPRATLRLLSKL 60

Query: 61  VFALFLPCLIFTHLGESITLQNIAKWWFIPVNVLISTGIGCLLGFLVVILCRPPPRLMRF 120
           VFALFLPCLIFTHLGE+ITL+NIAKWWF+PVNVLISTGIGCLLGFLVVI+CRPPP+L+RF
Sbjct: 61  VFALFLPCLIFTHLGENITLENIAKWWFVPVNVLISTGIGCLLGFLVVIICRPPPQLVRF 120

Query: 121 TVISTAFGNTGNLPLAIVGSVCHTADNPFGQHCHSRGVSYVSFCQWVSVILAYTLVYHMM 180
           T+ISTAFGNTGNLPLAIV SVCHT DNPFG++CH++GVSYVSFCQWVSVI+AYT VYHMM
Sbjct: 121 TIISTAFGNTGNLPLAIVSSVCHTDDNPFGKNCHTKGVSYVSFCQWVSVIIAYTFVYHMM 180

Query: 181 EPPLEFYEIVEEGTEIEELEQISDNDASKPLLIEAEWPGIEEKETEHCKAPFIARIFKSI 240
           EPPLEFYEIVEEGTEIEEL +  DND SKPLL+EAEWPGIEEKETEHCKAPFIAR+F SI
Sbjct: 181 EPPLEFYEIVEEGTEIEELVESDDNDVSKPLLMEAEWPGIEEKETEHCKAPFIARVFNSI 240

Query: 241 SNVSQSTFPDLDHSRESST--PTYPESIRCLAEPRVVRKIRIVAVQTPLKHILQPPTIAS 300
           SNVSQSTFPDLDH R+ ST    YPESIRCLAEPRVVRKIRIVA QTP++HILQPPTIAS
Sbjct: 241 SNVSQSTFPDLDHPRDGSTSASAYPESIRCLAEPRVVRKIRIVAEQTPIQHILQPPTIAS 300

Query: 301 LLAIIVGLVPQIRASFFGPDAPLSFISDSLEILAAAVVPFVMLMLGGMLAEGPHELSTLG 360
           LLAIIVGLVPQI+A  FG DAPL FIS+SLEI A A+VPFVML+LGGMLAEGP+E STLG
Sbjct: 301 LLAIIVGLVPQIKAVLFGADAPLLFISNSLEIFAGAMVPFVMLILGGMLAEGPNESSTLG 360

Query: 361 LRTTIGISVARLFVLPILGIGIVVSADKLNFLVDGDPMYKFVLLLQYTTPTAILLGAIAS 420
            RTTIGI VARL VLP+LGIGIV++ADKLNFLV+GDPMYKFVLLLQYTTPTAILLGA+AS
Sbjct: 361 QRTTIGICVARLLVLPVLGIGIVIAADKLNFLVNGDPMYKFVLLLQYTTPTAILLGALAS 420

Query: 421 LRGYAVREASALLFWEHIFALLSLSLYLFVYFKIVI 454
           LRGYAV+EASALLFWEHIFALLSLSLY+FVYFK+VI
Sbjct: 421 LRGYAVKEASALLFWEHIFALLSLSLYVFVYFKVVI 456

BLAST of Moc04g27390 vs. TAIR 10
Match: AT1G71090.1 (Auxin efflux carrier family protein )

HSP 1 Score: 615.1 bits (1585), Expect = 4.3e-176
Identity = 314/443 (70.88%), Postives = 380/443 (85.78%), Query Frame = 0

Query: 13  VRSSSEDLLSAITPLMKLLSLTAIGLLLSHPKVQMIPRATLRLLSKLVFALFLPCLIFTH 72
           V S   D+LS + PL+KL+ LT IGLLL+HPK Q++PRAT RLLSKLVFALFLPCLIFT 
Sbjct: 9   VNSRVVDILSGVVPLLKLICLTVIGLLLAHPKTQLVPRATFRLLSKLVFALFLPCLIFTE 68

Query: 73  LGESITLQNIAKWWFIPVNVLISTGIGCLLGFLVVILCRPPPRLMRFTVISTAFGNTGNL 132
           LGESITL NI +WWFIPVNVL+S  +G L+G+LVV++CRPPP   RFT++ TAFGNTGNL
Sbjct: 69  LGESITLDNIVQWWFIPVNVLLSAVVGSLIGYLVVLICRPPPEFNRFTIVMTAFGNTGNL 128

Query: 133 PLAIVGSVCHTADNPFGQHCHSRGVSYVSFCQWVSVILAYTLVYHMMEPPLEFYEIV-EE 192
            LAIV SVCHT  NPFG +C+SRGVSYVSF QWV+VIL YT+VYHMMEPPLE+YE+V EE
Sbjct: 129 LLAIVSSVCHTKTNPFGPNCNSRGVSYVSFAQWVAVILVYTVVYHMMEPPLEYYEVVEEE 188

Query: 193 GTEIEELEQISDNDASKPLLIEAEWPGIEEKETEHCKAPFIARIFKSISNVSQSTFPDLD 252
           G EIEE+  + ++DAS+PLL+EAEWPGIE+KETEHCK PFIAR+F SIS+ SQ++FP++D
Sbjct: 189 GVEIEEI-NVENHDASRPLLVEAEWPGIEDKETEHCKTPFIARVFNSISSFSQTSFPEVD 248

Query: 253 HSRE-SSTPTYPESIRCLAEPRVVRKIRIVAVQTPLKHILQPPTIASLLAIIVGLVPQIR 312
              E     + P SI+CLAEPRV+R+IR+VA QTP+KHILQPPTIASLLAII+G VPQ++
Sbjct: 249 LGGEYGGESSSPRSIQCLAEPRVMRRIRVVAEQTPVKHILQPPTIASLLAIIIGSVPQLK 308

Query: 313 ASFFGPDAPLSFISDSLEILAAAVVPFVMLMLGGMLAEGPHELSTLGLRTTIGISVARLF 372
           +  FG DAPLSFI+DSL I+ +A+VP VML+LGGML+EGP+E STLGLRTTIGISVARL 
Sbjct: 309 SVVFGYDAPLSFITDSLNIMGSAMVPSVMLVLGGMLSEGPNE-STLGLRTTIGISVARLL 368

Query: 373 VLPILGIGIVVSADKLNFLVDGDPMYKFVLLLQYTTPTAILLGAIASLRGYAVREASALL 432
           VLP++GIGIV+SADKL  +   DPM+KFVLLLQY+TP+AILLGAIASLRGYAVREASALL
Sbjct: 369 VLPLVGIGIVMSADKLGLISSADPMFKFVLLLQYSTPSAILLGAIASLRGYAVREASALL 428

Query: 433 FWEHIFALLSLSLYLFVYFKIVI 454
           FW+HIFALLSL+ Y+ ++FK+ +
Sbjct: 429 FWQHIFALLSLTFYIVIFFKLTV 449

BLAST of Moc04g27390 vs. TAIR 10
Match: AT5G01990.1 (Auxin efflux carrier family protein )

HSP 1 Score: 275.4 bits (703), Expect = 8.2e-74
Identity = 165/432 (38.19%), Postives = 252/432 (58.33%), Query Frame = 0

Query: 23  AITPLMKLLSLTAIGLLLSHPKVQMIPRATLRLLSKLVFALFLPCLIFTHLGESITLQNI 82
           A+ P+ K+ ++  +GLL++   V ++P +  +LL+ LVF+L LPCLIF+ LG+++TLQ +
Sbjct: 31  AVMPIAKVFTMCFLGLLMASKYVNILPPSGRKLLNGLVFSLLLPCLIFSQLGQAVTLQKM 90

Query: 83  AKWWFIPVNVLISTGIGCLLGFLVVILCRPPPRLMRFTVISTAFGNTGNLPLAIVGSVCH 142
            +WWFIPVNV++ T  G ++GF+V  + RPP    +FT+I    GN GN+PL ++ ++C 
Sbjct: 91  LQWWFIPVNVVLGTISGSIIGFIVASIVRPPYPYFKFTIIQIGVGNIGNVPLVLLAALCR 150

Query: 143 TADNPFG--QHCHSRGVSYVSFCQWVSVILAYTLVYHMMEPPLEFYEIVEEGTEIEELEQ 202
              NPFG  + C   G +Y+SF QWV  I+ YT VY M  PP E ++  EE         
Sbjct: 151 DTSNPFGDSEKCSIDGTAYISFGQWVGAIILYTYVYQMFAPPPEGFDAEEE--------- 210

Query: 203 ISDNDASKPLLIEAEWPGIEEKETEHCKAPFIARIFKSISNVSQSTFPDLDHSRESSTPT 262
              N A K L ++A             + P + + F    + +Q   P           T
Sbjct: 211 ---NLALKTLPVDAAPE----------QVPLLTQNFPKDFSPTQDLLP--------VQST 270

Query: 263 YPESIRCLAEPRVVRKIRIVAVQTPLKHILQPPTIASLLAIIVGLVPQIRASFFGPDAPL 322
            P       + ++ +    +  +  LK I+QP  +AS+LA+I+G +P  +   F   APL
Sbjct: 271 EPRGRGVSRKGKIAQIFVFLYEKLKLKQIVQPAIVASILAMILGAIPFTKKLIFTNGAPL 330

Query: 323 SFISDSLEILAAAVVPFVMLMLGGMLAEGPHELSTLGLRTTIGISVARLFVLPILGIGIV 382
            F +DS  IL  A++P ++L LGG L  GP   S LG +TT  I + RL ++P +G+GIV
Sbjct: 331 FFFTDSCMILGDAMIPCILLALGGNLINGPGS-SKLGFKTTAAIIIGRLVLVPPVGLGIV 390

Query: 383 VSADKLNFLVDGDPMYKFVLLLQYTTPTAILLGAIASLRGYAVREASALLFWEHIFALLS 442
             ADKL FL   D M++FVLLLQ+T PT++L GA+A+LRG   RE++A+LFW HIFA+ S
Sbjct: 391 TVADKLGFLPADDKMFRFVLLLQHTMPTSVLSGAVANLRGCG-RESAAVLFWVHIFAIFS 430

Query: 443 LSLYLFVYFKIV 453
           ++ ++ +Y  I+
Sbjct: 451 MAGWMVLYINIL 430

BLAST of Moc04g27390 vs. TAIR 10
Match: AT1G76520.1 (Auxin efflux carrier family protein )

HSP 1 Score: 183.0 bits (463), Expect = 5.5e-46
Identity = 125/429 (29.14%), Postives = 224/429 (52.21%), Query Frame = 0

Query: 18  EDLLSAITPLMKLLSLTAIGLLLSHPKVQMIPRATLRLLSKLVFALFLPCLIFTHLGESI 77
           E  +++  P++++L +T++G  ++   V ++     + L+ +VF +F P LI + L +S+
Sbjct: 6   ELFITSSKPVVEILLITSVGFYMALDGVNLLGHDARKYLNNIVFYVFSPSLIGSRLADSV 65

Query: 78  TLQNIAKWWFIPVNVLISTGIGCLLGFLVVILCRPPPRLMRFTVISTAFGNTGNLPLAIV 137
           T +++ K WF+PVNVL++  IG LLG++V+++ +PP  L    +   A GN GN+PL I+
Sbjct: 66  TYESLVKMWFMPVNVLLTFIIGSLLGWIVIVITKPPSHLRGLILGCCAAGNLGNMPLIII 125

Query: 138 GSVCHTADNPFG--QHCHSRGVSYVSFCQWVSVILAYTLVYHMMEPPLEFYEIVEEGTEI 197
            +VC     PFG  + C   G+ YV+    +  I  +T VY++M                
Sbjct: 126 PAVCKEKGGPFGDPESCQKYGMGYVALSMAMGSIYIWTYVYNLM---------------- 185

Query: 198 EELEQISDNDASKPLLIEAEWPGIEEKETEHCKAPFIARIFKSISNVSQSTFPDLDHSRE 257
                +S++    P  +E+ +        +  K P I+                   S+E
Sbjct: 186 ---RVLSNSPVETPPSVESNY--------DSYKVPLIS-------------------SKE 245

Query: 258 SSTPTYPESIRCLAEPRVVRKIRIVAVQTPLKHILQPPTIASLLAIIVGLVPQIRASFFG 317
                   + +     +V R++  ++ +  LK I  P TIA+++A+++GL+  +R    G
Sbjct: 246 EE-----NNQKAGRWEKVKRRLVSLSQKVNLKTIFAPSTIAAMIALVIGLITPLRKLIIG 305

Query: 318 PDAPLSFISDSLEILAAAVVPFVMLMLGGMLAEGPHELSTLGLRTTIGISVARLFVLPIL 377
            +APL  + DS+ ++    VP + +++GG L +G    S + + + IG+ VAR  +LP+ 
Sbjct: 306 TEAPLRVLQDSVTLVGDGAVPAMTMIIGGNLLKGLRS-SGMKMSSIIGVLVARYVLLPMS 365

Query: 378 GIGIVVSADKLNFLVDGDPMYKFVLLLQYTTPTAILLGAIASLRGYAVREASALLFWEHI 437
           G+ IV  A KL+ LV  +P+Y+FVLLLQY  P A+ LG I  L G    E S ++ W + 
Sbjct: 366 GVLIVRGAYKLD-LVTSEPLYQFVLLLQYAVPPAMNLGTITQLFGTGESECSVIMLWTYS 381

Query: 438 FALLSLSLY 445
            A ++L+++
Sbjct: 426 LASIALTVW 381

BLAST of Moc04g27390 vs. TAIR 10
Match: AT1G76520.2 (Auxin efflux carrier family protein )

HSP 1 Score: 183.0 bits (463), Expect = 5.5e-46
Identity = 125/429 (29.14%), Postives = 224/429 (52.21%), Query Frame = 0

Query: 18  EDLLSAITPLMKLLSLTAIGLLLSHPKVQMIPRATLRLLSKLVFALFLPCLIFTHLGESI 77
           E  +++  P++++L +T++G  ++   V ++     + L+ +VF +F P LI + L +S+
Sbjct: 6   ELFITSSKPVVEILLITSVGFYMALDGVNLLGHDARKYLNNIVFYVFSPSLIGSRLADSV 65

Query: 78  TLQNIAKWWFIPVNVLISTGIGCLLGFLVVILCRPPPRLMRFTVISTAFGNTGNLPLAIV 137
           T +++ K WF+PVNVL++  IG LLG++V+++ +PP  L    +   A GN GN+PL I+
Sbjct: 66  TYESLVKMWFMPVNVLLTFIIGSLLGWIVIVITKPPSHLRGLILGCCAAGNLGNMPLIII 125

Query: 138 GSVCHTADNPFG--QHCHSRGVSYVSFCQWVSVILAYTLVYHMMEPPLEFYEIVEEGTEI 197
            +VC     PFG  + C   G+ YV+    +  I  +T VY++M                
Sbjct: 126 PAVCKEKGGPFGDPESCQKYGMGYVALSMAMGSIYIWTYVYNLM---------------- 185

Query: 198 EELEQISDNDASKPLLIEAEWPGIEEKETEHCKAPFIARIFKSISNVSQSTFPDLDHSRE 257
                +S++    P  +E+ +        +  K P I+                   S+E
Sbjct: 186 ---RVLSNSPVETPPSVESNY--------DSYKVPLIS-------------------SKE 245

Query: 258 SSTPTYPESIRCLAEPRVVRKIRIVAVQTPLKHILQPPTIASLLAIIVGLVPQIRASFFG 317
                   + +     +V R++  ++ +  LK I  P TIA+++A+++GL+  +R    G
Sbjct: 246 EE-----NNQKAGRWEKVKRRLVSLSQKVNLKTIFAPSTIAAMIALVIGLITPLRKLIIG 305

Query: 318 PDAPLSFISDSLEILAAAVVPFVMLMLGGMLAEGPHELSTLGLRTTIGISVARLFVLPIL 377
            +APL  + DS+ ++    VP + +++GG L +G    S + + + IG+ VAR  +LP+ 
Sbjct: 306 TEAPLRVLQDSVTLVGDGAVPAMTMIIGGNLLKGLRS-SGMKMSSIIGVLVARYVLLPMS 365

Query: 378 GIGIVVSADKLNFLVDGDPMYKFVLLLQYTTPTAILLGAIASLRGYAVREASALLFWEHI 437
           G+ IV  A KL+ LV  +P+Y+FVLLLQY  P A+ LG I  L G    E S ++ W + 
Sbjct: 366 GVLIVRGAYKLD-LVTSEPLYQFVLLLQYAVPPAMNLGTITQLFGTGESECSVIMLWTYS 381

Query: 438 FALLSLSLY 445
            A ++L+++
Sbjct: 426 LASIALTVW 381

BLAST of Moc04g27390 vs. TAIR 10
Match: AT2G17500.1 (Auxin efflux carrier family protein )

HSP 1 Score: 182.2 bits (461), Expect = 9.4e-46
Identity = 125/434 (28.80%), Postives = 223/434 (51.38%), Query Frame = 0

Query: 23  AITPLMKLLSLTAIGLLLSHPKVQMIPRATLRLLSKLVFALFLPCLIFTHLGESITLQNI 82
           A  P++++L ++ +G  ++  + ++ P      ++K+VF LF P L+F +L +++TL++I
Sbjct: 10  ASMPVIQVLFMSLVGAFMASDRCKLFPVEARNSMNKVVFVLFAPALMFANLAQTVTLEDI 69

Query: 83  AKWWFIPVNVLISTGIGCLLGFLVVILCRPPPRLMRFTVISTAFGNTGNLPLAIVGSVCH 142
             WWF+PVN+ ++  IG LLG+LVV + +PPP L    V + + GN GNLP+ +V ++C 
Sbjct: 70  ISWWFMPVNMGLTFLIGGLLGWLVVKILKPPPYLEGLIVATCSAGNMGNLPIILVPAICD 129

Query: 143 TADNPFGQH--CHSRGVSYVSFCQWVSVILAYTLVYHMMEPPLEFYEIVEEGTEIEELEQ 202
              +PFG    C + G+SY SF   +     +T  + +++      + +EE  +I     
Sbjct: 130 EDKSPFGNRSVCRTVGLSYASFSMALGGFYIWTYTFRLIKGSAMKVQAIEESEKIAIKSS 189

Query: 203 ISDNDAS-KPLLIEAEWPGIEEKETEHCKAPFIARIFKSISNVSQSTFPDLDHSRESSTP 262
            SD +A  K  L+ A     E+KE +  K                             T 
Sbjct: 190 NSDLEADHKTHLLGAP----EDKENKVVK---------------------------EKTG 249

Query: 263 TYPESIRCLAEPRVVRKIRIVAVQTPLKHILQPPTIASLLAIIVGLVPQIRASFFGPDAP 322
            + + +  L E               L+ +L PPT+ +++  I G V  +R    G DAP
Sbjct: 250 FWRKGVDFLHE--------------ILEELLAPPTLGAIIGFIFGAVRWLRNLIIGDDAP 309

Query: 323 LSFISDSLEILAAAVVPFVMLMLGGMLAEGPHELSTLGLRTTIGISVARLFVLPILGIGI 382
           L  +  + ++L    +P + ++LGG L +G    S +     +GI   R   +PI+GIGI
Sbjct: 310 LRIVQSTAKLLGDGTIPCMTIILGGNLIQGLRS-SAVKPMVVLGIVCVRYIAMPIIGIGI 369

Query: 383 VVSADKLNFLVDGDPMYKFVLLLQYTTPTAILLGAIASLRGYAVREASALLFWEHIFALL 442
           V++A  L FL   DP++++VL+LQ+T P A+ +G +  L   A  E S L+ W ++ A+L
Sbjct: 370 VLTAANLGFL-PADPLFQYVLMLQFTLPPAMNIGTMTQLYNVAQDECSVLMLWTYLVAIL 396

Query: 443 SLSLYLFVYFKIVI 454
           +L+++  ++  +++
Sbjct: 430 ALTVWSTIFLHLLV 396

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022152345.18.9e-248100.00protein PIN-LIKES 2-like [Momordica charantia][more]
XP_038875937.11.7e-22289.23protein PIN-LIKES 2 [Benincasa hispida][more]
XP_022939090.12.2e-22288.77protein PIN-LIKES 2 [Cucurbita moschata] >XP_022939092.1 protein PIN-LIKES 2 [Cu... [more]
KAG6579244.12.2e-22288.77Protein PIN-LIKES 2, partial [Cucurbita argyrosperma subsp. sororia][more]
XP_022993212.11.1e-22188.55protein PIN-LIKES 2 [Cucurbita maxima] >XP_022993213.1 protein PIN-LIKES 2 [Cucu... [more]
Match NameE-valueIdentityDescription
Q9C9996.1e-17570.88Protein PIN-LIKES 2 OS=Arabidopsis thaliana OX=3702 GN=PILS2 PE=2 SV=1[more]
Q9LZN21.1e-7238.19Protein PIN-LIKES 6 OS=Arabidopsis thaliana OX=3702 GN=PILS6 PE=2 SV=1[more]
Q9C9K57.8e-4529.14Protein PIN-LIKES 3 OS=Arabidopsis thaliana OX=3702 GN=PILS3 PE=2 SV=1[more]
Q9SHL81.3e-4428.80Protein PIN-LIKES 5 OS=Arabidopsis thaliana OX=3702 GN=PILS5 PE=2 SV=1[more]
F4HWB65.0e-4430.52Protein PIN-LIKES 1 OS=Arabidopsis thaliana OX=3702 GN=PILS1 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1DDP04.3e-248100.00protein PIN-LIKES 2-like OS=Momordica charantia OX=3673 GN=LOC111020086 PE=4 SV=... [more]
A0A6J1FFU81.1e-22288.77protein PIN-LIKES 2 OS=Cucurbita moschata OX=3662 GN=LOC111445095 PE=4 SV=1[more]
A0A6J1JS575.3e-22288.55protein PIN-LIKES 2 OS=Cucurbita maxima OX=3661 GN=LOC111489301 PE=4 SV=1[more]
A0A0A0KRM34.1e-21485.90Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G571470 PE=4 SV=1[more]
A0A5D3E4I55.3e-21485.31Protein PIN-LIKES 2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold426G... [more]
Match NameE-valueIdentityDescription
AT1G71090.14.3e-17670.88Auxin efflux carrier family protein [more]
AT5G01990.18.2e-7438.19Auxin efflux carrier family protein [more]
AT1G76520.15.5e-4629.14Auxin efflux carrier family protein [more]
AT1G76520.25.5e-4629.14Auxin efflux carrier family protein [more]
AT2G17500.19.4e-4628.80Auxin efflux carrier family protein [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (OHB3-1) v2
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR004776Membrane transport proteinPFAMPF03547Mem_transcoord: 23..445
e-value: 3.7E-89
score: 298.8
NoneNo IPR availablePANTHERPTHR31419:SF12AUXIN EFFLUX CARRIER FAMILY PROTEIN-RELATEDcoord: 10..452
IPR039305Protein PIN-LIKES 2/6PANTHERPTHR31419PROTEIN PIN-LIKES 2coord: 10..452

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Moc04g27390.1Moc04g27390.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0010315 auxin efflux
biological_process GO:0010252 auxin homeostasis
biological_process GO:0080162 intracellular auxin transport
biological_process GO:0010311 lateral root formation
biological_process GO:0040009 regulation of growth rate
biological_process GO:0055085 transmembrane transport
cellular_component GO:0005789 endoplasmic reticulum membrane
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0010329 auxin efflux transmembrane transporter activity