Moc04g27320 (gene) Bitter gourd (OHB3-1) v2

Overview
NameMoc04g27320
Typegene
OrganismMomordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
DescriptionRING-type E3 ubiquitin transferase
Locationchr4: 20153570 .. 20158515 (-)
RNA-Seq ExpressionMoc04g27320
SyntenyMoc04g27320
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGCAAAAGGACTGCACCGATCTCATCCGTCGGATCGCGCTCCTGATTCATCTGGCGGAGGAGATCATGAACTTCCGCCGCGGCGGCGACAATTTGGCGGAATTGAATGAGTCTGGTTCTTCTTCTTCTTGTTCTCTGGATTGCCTTTCGGAAGTGGTCGGAGCCATTCAGGCGGCGAAGCGCCTTCTCTACGCCGCTTTGACTTTCTCTGGAAACGACGACGCTTCTGCGGCTTCGACTGTGAGTTCATCATCCTCACTGTTCTTTCATTTTTATTTTGTTTGTGGTTAAATTCTAATTGTTTTTTGTGTTCTGATTGGAAAATTTACAAGCTGGAAGTGGAAATTCCATTCATATATGTAATAAGTGTTCATGCTGAGTTTATGATTTTCGGTGCAGTTTTGGCAAATTTTCTCGGCTGCCAAACGGTTCTGTTTGCTTTAGATTTTACGACAAATTTATTTCTACGTGCTGGCTGCTTCCTCCTTTGTTTTTCTCTTTCTTTCTTTTGCAGTTTTGTGTTCAATAAAAATAATACATAAATAATTAGGTTATCTCAATCCTATGATGTTAGATATTCTTATCTTACACTTTTGAAATTATTTTGGTGGTGTCACATTCGTGATACTGAACCAGGTCAATGAATCAATTCCGTTCTTTTTTCTTTTTTCTTTTTTTTTATGTTTTGTTCAATTTCGAACCAAATATGTTCGATTGATATTATTTTTAAAATGATTTATTCATTATATAATATATATATTGATTATTGTTTTATATATCGATTATGTATTAATATTTATTTACTGCTTTTATCATTTACCTATGTTTCATTAGGTGATATTTTTTAGTCAATTGATTTTTAATGAAGTAACTTTGGTGGTATTAATCTATATTTTTTAAAAAAAAAAAATTATTAAGGGTTTAAGTTTGGAATGATTTTGGGAGAGTTTTTCAATTTTTTTAATAAGTGTTGATTAAATATATATATTTTTTAAAGGGAAAAGTTCAAATTTTGCTCTTACTTAGTGAGTTAATTTTAACCTTAAACTTTTTAGTTCTATCAATTTAAACTCTAAATTTAGTATCATACAAAAGTAAAAAAAAAAAAAAATTTAAGTAACTCTCAAACTCAACCACAAAAATACTTGAACTTAACCACAAAATTGAGTAAATAAAAAATGCTTAAATTTAGTAAAAGAATGTTCAAATCCAACTAATAATAGTATGTAACACAATTAGAAGAATCAATACGTACAAAAATTGTCGCATCATGGTCTATAACTGCTATAAATCTTCACAAAGAAAAAGAAAATAGAACTTAACTAATTTTGGGGTATTAATTACATAATTTATTCAAGTTCAGGGGTGGAAATTAAAAGTTTAAAGTTGAAATTGCCTCCCCACCTAATTGGAGTAAAACTTAAAATTTTATCTTCTATAGGAATATTTTATAATTTTTTTTAGAATCTTTATTTTTTTTTTTTGACTATATAACTGAATAGCATGAAATATTTGCGCAGTGGTTTCAGTTAACCAATAGCTAAAATTGTGATGGAATCTATATCCTATGTTGATTTTTCACTGATAAAATAAGATAAGTAAATAAAATAAATAAATCATTTAAAAACGTAAATGACTATTTTGATTTTCAATATATAAATGTACGATTTTATTTTAGAAAGATCAAAAATAGTTTTTAACATTATTTCCAAACCCCTAAAATCATTGTAGAAACATTTTAGCCAATTATATATTTTTTTTGTTATATATTTTACATTTAAATGAGAGTAAAATCTCAATGAATATTTTATTTATAAAAATAAAAATTCTAAAATTTCTTATTCTAGTGCTTTATGAGTATTATCCAATACTCTTAAATTTATTCTTATACCATGGCTTCAGGTAAAAACTAAAATACTATTGAAACAAAAATAATTACTTCACACCTCATAAATAGGTTTAAATTCTTATTATTAATATTTTAAAACATTATATTTACAATATAAATTTAAAAAAAACGTTTAACTCTTAATATCAATCTAAAACTCAATTTATGAAAATATTGATACATTAATAAATGATTTGGAAAAAAGAAATTGGACACTGAAATTTCTTTGATGTTGTTAATTTGGAAGGATGGAGCTACCAAAAAATTGGTTTACCAATTTCAGTATGTGACAAACAGATTGCAAAATGCACTTGCAAATTTGCCATACGACCACTTTTGCATCTCAGATGAAGTTCAGGAACAGGTAAAACCAAAACTCAGGTGACCCGAATCTCGAGAAGTTTATTCTTTTTCTAAGTTTAACATGTGAGAGTGGAGATGGAGATTCGAATCTTTAACATTTTGGTTGAGGATTAATGTTTCCTTTAGTTGAGCTCTATGTTCAGATGGATGACTCAAGAAGTTTATGATACTAAAAACTATGGTGTGGTAAAATCCATTGGTAATTAATATGAGCGAGGAATAATAGCATTAGTAGAGAGGTTGAGAAAGTAGACAATTTTTACCTTGTTTGATGACCATTTTATTTTTTGTTTTCTACTTTTTTTTTTTTAATTTAGCTGATAAATACTACTTTCATCCATGTATTTCATTTGTTTGGATGTTATATACATTTTAAAAATGTTTTCAAAATTCACACAAAAAAAATGAAAACTAAAAGAAAAAATAAATTTTAAAACTTTTTTTTTTTGCTATAAAAAAATAGTTTTAAAAAACATTGAAAAATATACAAATTTTTTAGAAGGAAAGCATAATTTTTAAAAAAATAGAAAACCAAAAACTAAATTACTATTTTCTTAAGCTTATTAAACTATTTTTATTCATGTATTTATTTTATCTATCTCTTAAAAAGATTTTAAAAATCTAAGTCAAAATTTGTATTTTCTTTTAAATTTTGTTAAGGATTTAATTGTGTTTAAAACAAAGGAAAAAAGTACTAAAACTTGAGAGGAAGTCAACTTAATCCTTAAAAATTGAAAAATCTTTATCAAATTGCAGCAGTAATAAACCATCATGGATGACTAGTGGTCAATAAAGACCCTTGTAAATAGAAAAGGATTTAAAAGGAATGAGTTCAAAACATGGTGGCTACCTATCTAGGATTTAATATCCTATGAATTATCTTGTCAGTCAAATGTAGTAAAAATTGTAGCAGTAATAGTTGTCCCTAATTCAAGTTGATTATATTTCGTGGGAAAACTTTTTGCTTCCTAAGCTTTACTTTTGCATTTTCATTTTGTGGGTACAGGTTGATTTGGTTAGAGCTCAGTTGAGGAGAGCAGAAAAGAAGTATGAGTCCATGTCTGAACTTGCAAACAAGAAGCTTCATTCTCAAGGCTCTGTAAAATGGAAGGTCAACAATGATGTTACGAGCATGTCTAGTGTTGATTATGAAGTTCCCGAGTCGCAGCATCGTCCCCAGAACCGTGACTATCAGGTCGGTTTCGATCCGGAGGAGCCGAAGGCCTCGGATTTGTGTATGGATGAGTGTTCAAGTGTTGTTCAATCTGATATGGAAGATGTTCAAGCCACCAAGAGCCAGGAGGAGGTTGAGAAGTCTGATGAAATTATGATTCCTGAGAACTTCTTGTGCCCCATCTCCTTTGAATTGATGGTAGATCCCGTCATCATCTCGACAGGACAGGTACGATCGTCATCAATCCGATTATGTTATTTTCTTTCTATGTGTTACTTTAACTTATCATATTAAATTTTTGAGTTTAGTAATGATTTAATCCTACGAGAAGACGTGGAAGAAAAAGAAAACTACTTTTCAAGTTCATTATTAAAAAATGTTAATGGAATATTTTTTGACTAAATTACGATCCCGAACGGCCTGAACGGGGCTGAATTGACTATAGTTATGATCCATTTATAGACCTATGAGAGATCCAATATACAGAGGTGGATAGACAAAGGGAACAAAACTTGTCCAAAAACTCAGGAGCAGCTCCAAGCTTTGATCCTCACTCCAAATTTTCTCATGAAACAACTGATCTCTGAATGGTGCCAAGAACACAATGTCAAGCTAGAGGAAGGATTAACCACCAGAAAAATCAAGAAGTACAGATCATTCGAAGATGGTTCTCGAAAGAGGGTACCCGTCAAAACTCTAGTTCGACACCTCTCTTTCGGGTCGGCTCATGAGAAAAAGGCAGCAGTGACAGAGATCCGAAAACTAACGAAAAGTAGCTCAGAACATAGAGTTGAAATAGCAGAGGCAGGAGCAATCCCACAGCTGGTTAACCTTTTAACTTCAGAAGATGTTCTAACACAAGAGAATGCAATTTCTTGCATTTTGAATCTTTCACTTCATGAGCAGAACAAGAGACTTGTTATGCTTTCTGGTGCAGTTTCCTACATTTCCCAAGTCCTAAAATTTGGGAGCTTGGAAGCGAGAGAGTGTGCTGCTGCGACGCTTTACAGCTTGTCGTTAGCCGACGAGAACAAGGCGGTCATCGGGGCTTCGGGCGTGATCCCGGGCCTGTTGGAAATTCTCGAGATCGGGAGTGCAAGAGGGAAGAAAGATGCAGCAAGGGCCCTGTTGAATTTGTGTATGTACCAAGGGAACAAAAGCAGGGCTGTGAAGGCTGGCATAGTCAGACCATTGTTGAAATTGCTCTCAGATTCAGATGCCTCCATGGTTGATGAAGCGCTGTATATAATGTCGGTTCTTTGCGTCCATCCGGAGGCGAAGGCAGCAATGGCGAATGCGAATTCGCTTCTCGTTCTGACCGAGGTTCTGAAGACGGGGGCGTCTCGGAGCAAGGAAAATGCAGCTGCAGTTATGTTTGTATTGTGCAAGGGAGATTTGGAGAAGCTTTCATGGTTAACCAAGCTTGGCGCAACGATACCGTTGATGATACTCGCCGAGAACGGCACGGGGAGAGCGAGGCGGAAGGCTACTTCATTGTTGGAGGAACTCAGAAGATCATGA

mRNA sequence

ATGCAAAAGGACTGCACCGATCTCATCCGTCGGATCGCGCTCCTGATTCATCTGGCGGAGGAGATCATGAACTTCCGCCGCGGCGGCGACAATTTGGCGGAATTGAATGAGTCTGGTTCTTCTTCTTCTTGTTCTCTGGATTGCCTTTCGGAAGTGGTCGGAGCCATTCAGGCGGCGAAGCGCCTTCTCTACGCCGCTTTGACTTTCTCTGGAAACGACGACGCTTCTGCGGCTTCGACTGATGGAGCTACCAAAAAATTGGTTTACCAATTTCAGTATGTGACAAACAGATTGCAAAATGCACTTGCAAATTTGCCATACGACCACTTTTGCATCTCAGATGAAGTTCAGGAACAGGTTGATTTGGTTAGAGCTCAGTTGAGGAGAGCAGAAAAGAAGTATGAGTCCATGTCTGAACTTGCAAACAAGAAGCTTCATTCTCAAGGCTCTGTAAAATGGAAGGTCAACAATGATGTTACGAGCATGTCTAGTGTTGATTATGAAGTTCCCGAGTCGCAGCATCGTCCCCAGAACCGTGACTATCAGGTCGGTTTCGATCCGGAGGAGCCGAAGGCCTCGGATTTGTGTATGGATGAGTGTTCAAGTGTTGTTCAATCTGATATGGAAGATGTTCAAGCCACCAAGAGCCAGGAGGAGGTTGAGAAGTCTGATGAAATTATGATTCCTGAGAACTTCTTGTGCCCCATCTCCTTTGAATTGATGGTAGATCCCGTCATCATCTCGACAGGACAGACCTATGAGAGATCCAATATACAGAGGTGGATAGACAAAGGGAACAAAACTTGTCCAAAAACTCAGGAGCAGCTCCAAGCTTTGATCCTCACTCCAAATTTTCTCATGAAACAACTGATCTCTGAATGGTGCCAAGAACACAATGTCAAGCTAGAGGAAGGATTAACCACCAGAAAAATCAAGAAGTACAGATCATTCGAAGATGGTTCTCGAAAGAGGGTACCCGTCAAAACTCTAGTTCGACACCTCTCTTTCGGGTCGGCTCATGAGAAAAAGGCAGCAGTGACAGAGATCCGAAAACTAACGAAAAGTAGCTCAGAACATAGAGTTGAAATAGCAGAGGCAGGAGCAATCCCACAGCTGGTTAACCTTTTAACTTCAGAAGATGTTCTAACACAAGAGAATGCAATTTCTTGCATTTTGAATCTTTCACTTCATGAGCAGAACAAGAGACTTGTTATGCTTTCTGGTGCAGTTTCCTACATTTCCCAAGTCCTAAAATTTGGGAGCTTGGAAGCGAGAGAGTGTGCTGCTGCGACGCTTTACAGCTTGTCGTTAGCCGACGAGAACAAGGCGGTCATCGGGGCTTCGGGCGTGATCCCGGGCCTGTTGGAAATTCTCGAGATCGGGAGTGCAAGAGGGAAGAAAGATGCAGCAAGGGCCCTGTTGAATTTGTGTATGTACCAAGGGAACAAAAGCAGGGCTGTGAAGGCTGGCATAGTCAGACCATTGTTGAAATTGCTCTCAGATTCAGATGCCTCCATGGTTGATGAAGCGCTGTATATAATGTCGGTTCTTTGCGTCCATCCGGAGGCGAAGGCAGCAATGGCGAATGCGAATTCGCTTCTCGTTCTGACCGAGGTTCTGAAGACGGGGGCGTCTCGGAGCAAGGAAAATGCAGCTGCAGTTATGTTTGTATTGTGCAAGGGAGATTTGGAGAAGCTTTCATGGTTAACCAAGCTTGGCGCAACGATACCGTTGATGATACTCGCCGAGAACGGCACGGGGAGAGCGAGGCGGAAGGCTACTTCATTGTTGGAGGAACTCAGAAGATCATGA

Coding sequence (CDS)

ATGCAAAAGGACTGCACCGATCTCATCCGTCGGATCGCGCTCCTGATTCATCTGGCGGAGGAGATCATGAACTTCCGCCGCGGCGGCGACAATTTGGCGGAATTGAATGAGTCTGGTTCTTCTTCTTCTTGTTCTCTGGATTGCCTTTCGGAAGTGGTCGGAGCCATTCAGGCGGCGAAGCGCCTTCTCTACGCCGCTTTGACTTTCTCTGGAAACGACGACGCTTCTGCGGCTTCGACTGATGGAGCTACCAAAAAATTGGTTTACCAATTTCAGTATGTGACAAACAGATTGCAAAATGCACTTGCAAATTTGCCATACGACCACTTTTGCATCTCAGATGAAGTTCAGGAACAGGTTGATTTGGTTAGAGCTCAGTTGAGGAGAGCAGAAAAGAAGTATGAGTCCATGTCTGAACTTGCAAACAAGAAGCTTCATTCTCAAGGCTCTGTAAAATGGAAGGTCAACAATGATGTTACGAGCATGTCTAGTGTTGATTATGAAGTTCCCGAGTCGCAGCATCGTCCCCAGAACCGTGACTATCAGGTCGGTTTCGATCCGGAGGAGCCGAAGGCCTCGGATTTGTGTATGGATGAGTGTTCAAGTGTTGTTCAATCTGATATGGAAGATGTTCAAGCCACCAAGAGCCAGGAGGAGGTTGAGAAGTCTGATGAAATTATGATTCCTGAGAACTTCTTGTGCCCCATCTCCTTTGAATTGATGGTAGATCCCGTCATCATCTCGACAGGACAGACCTATGAGAGATCCAATATACAGAGGTGGATAGACAAAGGGAACAAAACTTGTCCAAAAACTCAGGAGCAGCTCCAAGCTTTGATCCTCACTCCAAATTTTCTCATGAAACAACTGATCTCTGAATGGTGCCAAGAACACAATGTCAAGCTAGAGGAAGGATTAACCACCAGAAAAATCAAGAAGTACAGATCATTCGAAGATGGTTCTCGAAAGAGGGTACCCGTCAAAACTCTAGTTCGACACCTCTCTTTCGGGTCGGCTCATGAGAAAAAGGCAGCAGTGACAGAGATCCGAAAACTAACGAAAAGTAGCTCAGAACATAGAGTTGAAATAGCAGAGGCAGGAGCAATCCCACAGCTGGTTAACCTTTTAACTTCAGAAGATGTTCTAACACAAGAGAATGCAATTTCTTGCATTTTGAATCTTTCACTTCATGAGCAGAACAAGAGACTTGTTATGCTTTCTGGTGCAGTTTCCTACATTTCCCAAGTCCTAAAATTTGGGAGCTTGGAAGCGAGAGAGTGTGCTGCTGCGACGCTTTACAGCTTGTCGTTAGCCGACGAGAACAAGGCGGTCATCGGGGCTTCGGGCGTGATCCCGGGCCTGTTGGAAATTCTCGAGATCGGGAGTGCAAGAGGGAAGAAAGATGCAGCAAGGGCCCTGTTGAATTTGTGTATGTACCAAGGGAACAAAAGCAGGGCTGTGAAGGCTGGCATAGTCAGACCATTGTTGAAATTGCTCTCAGATTCAGATGCCTCCATGGTTGATGAAGCGCTGTATATAATGTCGGTTCTTTGCGTCCATCCGGAGGCGAAGGCAGCAATGGCGAATGCGAATTCGCTTCTCGTTCTGACCGAGGTTCTGAAGACGGGGGCGTCTCGGAGCAAGGAAAATGCAGCTGCAGTTATGTTTGTATTGTGCAAGGGAGATTTGGAGAAGCTTTCATGGTTAACCAAGCTTGGCGCAACGATACCGTTGATGATACTCGCCGAGAACGGCACGGGGAGAGCGAGGCGGAAGGCTACTTCATTGTTGGAGGAACTCAGAAGATCATGA

Protein sequence

MQKDCTDLIRRIALLIHLAEEIMNFRRGGDNLAELNESGSSSSCSLDCLSEVVGAIQAAKRLLYAALTFSGNDDASAASTDGATKKLVYQFQYVTNRLQNALANLPYDHFCISDEVQEQVDLVRAQLRRAEKKYESMSELANKKLHSQGSVKWKVNNDVTSMSSVDYEVPESQHRPQNRDYQVGFDPEEPKASDLCMDECSSVVQSDMEDVQATKSQEEVEKSDEIMIPENFLCPISFELMVDPVIISTGQTYERSNIQRWIDKGNKTCPKTQEQLQALILTPNFLMKQLISEWCQEHNVKLEEGLTTRKIKKYRSFEDGSRKRVPVKTLVRHLSFGSAHEKKAAVTEIRKLTKSSSEHRVEIAEAGAIPQLVNLLTSEDVLTQENAISCILNLSLHEQNKRLVMLSGAVSYISQVLKFGSLEARECAAATLYSLSLADENKAVIGASGVIPGLLEILEIGSARGKKDAARALLNLCMYQGNKSRAVKAGIVRPLLKLLSDSDASMVDEALYIMSVLCVHPEAKAAMANANSLLVLTEVLKTGASRSKENAAAVMFVLCKGDLEKLSWLTKLGATIPLMILAENGTGRARRKATSLLEELRRS
Homology
BLAST of Moc04g27320 vs. NCBI nr
Match: XP_022152334.1 (U-box domain-containing protein 11-like isoform X1 [Momordica charantia])

HSP 1 Score: 1145.2 bits (2961), Expect = 0.0e+00
Identity = 603/603 (100.00%), Postives = 603/603 (100.00%), Query Frame = 0

Query: 1   MQKDCTDLIRRIALLIHLAEEIMNFRRGGDNLAELNESGSSSSCSLDCLSEVVGAIQAAK 60
           MQKDCTDLIRRIALLIHLAEEIMNFRRGGDNLAELNESGSSSSCSLDCLSEVVGAIQAAK
Sbjct: 40  MQKDCTDLIRRIALLIHLAEEIMNFRRGGDNLAELNESGSSSSCSLDCLSEVVGAIQAAK 99

Query: 61  RLLYAALTFSGNDDASAASTDGATKKLVYQFQYVTNRLQNALANLPYDHFCISDEVQEQV 120
           RLLYAALTFSGNDDASAASTDGATKKLVYQFQYVTNRLQNALANLPYDHFCISDEVQEQV
Sbjct: 100 RLLYAALTFSGNDDASAASTDGATKKLVYQFQYVTNRLQNALANLPYDHFCISDEVQEQV 159

Query: 121 DLVRAQLRRAEKKYESMSELANKKLHSQGSVKWKVNNDVTSMSSVDYEVPESQHRPQNRD 180
           DLVRAQLRRAEKKYESMSELANKKLHSQGSVKWKVNNDVTSMSSVDYEVPESQHRPQNRD
Sbjct: 160 DLVRAQLRRAEKKYESMSELANKKLHSQGSVKWKVNNDVTSMSSVDYEVPESQHRPQNRD 219

Query: 181 YQVGFDPEEPKASDLCMDECSSVVQSDMEDVQATKSQEEVEKSDEIMIPENFLCPISFEL 240
           YQVGFDPEEPKASDLCMDECSSVVQSDMEDVQATKSQEEVEKSDEIMIPENFLCPISFEL
Sbjct: 220 YQVGFDPEEPKASDLCMDECSSVVQSDMEDVQATKSQEEVEKSDEIMIPENFLCPISFEL 279

Query: 241 MVDPVIISTGQTYERSNIQRWIDKGNKTCPKTQEQLQALILTPNFLMKQLISEWCQEHNV 300
           MVDPVIISTGQTYERSNIQRWIDKGNKTCPKTQEQLQALILTPNFLMKQLISEWCQEHNV
Sbjct: 280 MVDPVIISTGQTYERSNIQRWIDKGNKTCPKTQEQLQALILTPNFLMKQLISEWCQEHNV 339

Query: 301 KLEEGLTTRKIKKYRSFEDGSRKRVPVKTLVRHLSFGSAHEKKAAVTEIRKLTKSSSEHR 360
           KLEEGLTTRKIKKYRSFEDGSRKRVPVKTLVRHLSFGSAHEKKAAVTEIRKLTKSSSEHR
Sbjct: 340 KLEEGLTTRKIKKYRSFEDGSRKRVPVKTLVRHLSFGSAHEKKAAVTEIRKLTKSSSEHR 399

Query: 361 VEIAEAGAIPQLVNLLTSEDVLTQENAISCILNLSLHEQNKRLVMLSGAVSYISQVLKFG 420
           VEIAEAGAIPQLVNLLTSEDVLTQENAISCILNLSLHEQNKRLVMLSGAVSYISQVLKFG
Sbjct: 400 VEIAEAGAIPQLVNLLTSEDVLTQENAISCILNLSLHEQNKRLVMLSGAVSYISQVLKFG 459

Query: 421 SLEARECAAATLYSLSLADENKAVIGASGVIPGLLEILEIGSARGKKDAARALLNLCMYQ 480
           SLEARECAAATLYSLSLADENKAVIGASGVIPGLLEILEIGSARGKKDAARALLNLCMYQ
Sbjct: 460 SLEARECAAATLYSLSLADENKAVIGASGVIPGLLEILEIGSARGKKDAARALLNLCMYQ 519

Query: 481 GNKSRAVKAGIVRPLLKLLSDSDASMVDEALYIMSVLCVHPEAKAAMANANSLLVLTEVL 540
           GNKSRAVKAGIVRPLLKLLSDSDASMVDEALYIMSVLCVHPEAKAAMANANSLLVLTEVL
Sbjct: 520 GNKSRAVKAGIVRPLLKLLSDSDASMVDEALYIMSVLCVHPEAKAAMANANSLLVLTEVL 579

Query: 541 KTGASRSKENAAAVMFVLCKGDLEKLSWLTKLGATIPLMILAENGTGRARRKATSLLEEL 600
           KTGASRSKENAAAVMFVLCKGDLEKLSWLTKLGATIPLMILAENGTGRARRKATSLLEEL
Sbjct: 580 KTGASRSKENAAAVMFVLCKGDLEKLSWLTKLGATIPLMILAENGTGRARRKATSLLEEL 639

Query: 601 RRS 604
           RRS
Sbjct: 640 RRS 642

BLAST of Moc04g27320 vs. NCBI nr
Match: XP_022152335.1 (U-box domain-containing protein 11-like isoform X2 [Momordica charantia])

HSP 1 Score: 1145.2 bits (2961), Expect = 0.0e+00
Identity = 603/603 (100.00%), Postives = 603/603 (100.00%), Query Frame = 0

Query: 1   MQKDCTDLIRRIALLIHLAEEIMNFRRGGDNLAELNESGSSSSCSLDCLSEVVGAIQAAK 60
           MQKDCTDLIRRIALLIHLAEEIMNFRRGGDNLAELNESGSSSSCSLDCLSEVVGAIQAAK
Sbjct: 1   MQKDCTDLIRRIALLIHLAEEIMNFRRGGDNLAELNESGSSSSCSLDCLSEVVGAIQAAK 60

Query: 61  RLLYAALTFSGNDDASAASTDGATKKLVYQFQYVTNRLQNALANLPYDHFCISDEVQEQV 120
           RLLYAALTFSGNDDASAASTDGATKKLVYQFQYVTNRLQNALANLPYDHFCISDEVQEQV
Sbjct: 61  RLLYAALTFSGNDDASAASTDGATKKLVYQFQYVTNRLQNALANLPYDHFCISDEVQEQV 120

Query: 121 DLVRAQLRRAEKKYESMSELANKKLHSQGSVKWKVNNDVTSMSSVDYEVPESQHRPQNRD 180
           DLVRAQLRRAEKKYESMSELANKKLHSQGSVKWKVNNDVTSMSSVDYEVPESQHRPQNRD
Sbjct: 121 DLVRAQLRRAEKKYESMSELANKKLHSQGSVKWKVNNDVTSMSSVDYEVPESQHRPQNRD 180

Query: 181 YQVGFDPEEPKASDLCMDECSSVVQSDMEDVQATKSQEEVEKSDEIMIPENFLCPISFEL 240
           YQVGFDPEEPKASDLCMDECSSVVQSDMEDVQATKSQEEVEKSDEIMIPENFLCPISFEL
Sbjct: 181 YQVGFDPEEPKASDLCMDECSSVVQSDMEDVQATKSQEEVEKSDEIMIPENFLCPISFEL 240

Query: 241 MVDPVIISTGQTYERSNIQRWIDKGNKTCPKTQEQLQALILTPNFLMKQLISEWCQEHNV 300
           MVDPVIISTGQTYERSNIQRWIDKGNKTCPKTQEQLQALILTPNFLMKQLISEWCQEHNV
Sbjct: 241 MVDPVIISTGQTYERSNIQRWIDKGNKTCPKTQEQLQALILTPNFLMKQLISEWCQEHNV 300

Query: 301 KLEEGLTTRKIKKYRSFEDGSRKRVPVKTLVRHLSFGSAHEKKAAVTEIRKLTKSSSEHR 360
           KLEEGLTTRKIKKYRSFEDGSRKRVPVKTLVRHLSFGSAHEKKAAVTEIRKLTKSSSEHR
Sbjct: 301 KLEEGLTTRKIKKYRSFEDGSRKRVPVKTLVRHLSFGSAHEKKAAVTEIRKLTKSSSEHR 360

Query: 361 VEIAEAGAIPQLVNLLTSEDVLTQENAISCILNLSLHEQNKRLVMLSGAVSYISQVLKFG 420
           VEIAEAGAIPQLVNLLTSEDVLTQENAISCILNLSLHEQNKRLVMLSGAVSYISQVLKFG
Sbjct: 361 VEIAEAGAIPQLVNLLTSEDVLTQENAISCILNLSLHEQNKRLVMLSGAVSYISQVLKFG 420

Query: 421 SLEARECAAATLYSLSLADENKAVIGASGVIPGLLEILEIGSARGKKDAARALLNLCMYQ 480
           SLEARECAAATLYSLSLADENKAVIGASGVIPGLLEILEIGSARGKKDAARALLNLCMYQ
Sbjct: 421 SLEARECAAATLYSLSLADENKAVIGASGVIPGLLEILEIGSARGKKDAARALLNLCMYQ 480

Query: 481 GNKSRAVKAGIVRPLLKLLSDSDASMVDEALYIMSVLCVHPEAKAAMANANSLLVLTEVL 540
           GNKSRAVKAGIVRPLLKLLSDSDASMVDEALYIMSVLCVHPEAKAAMANANSLLVLTEVL
Sbjct: 481 GNKSRAVKAGIVRPLLKLLSDSDASMVDEALYIMSVLCVHPEAKAAMANANSLLVLTEVL 540

Query: 541 KTGASRSKENAAAVMFVLCKGDLEKLSWLTKLGATIPLMILAENGTGRARRKATSLLEEL 600
           KTGASRSKENAAAVMFVLCKGDLEKLSWLTKLGATIPLMILAENGTGRARRKATSLLEEL
Sbjct: 541 KTGASRSKENAAAVMFVLCKGDLEKLSWLTKLGATIPLMILAENGTGRARRKATSLLEEL 600

Query: 601 RRS 604
           RRS
Sbjct: 601 RRS 603

BLAST of Moc04g27320 vs. NCBI nr
Match: XP_038875665.1 (U-box domain-containing protein 11-like isoform X3 [Benincasa hispida])

HSP 1 Score: 878.6 bits (2269), Expect = 3.0e-251
Identity = 470/607 (77.43%), Postives = 524/607 (86.33%), Query Frame = 0

Query: 1   MQKDCTDLIRRIALLIHLAEEIMNFRRG-GDNLAELNESG-SSSSCSLDCLSEVVGAIQA 60
           M+KDCTDLIRRIALLIHLAEEI NF  G G+N   LN+ G SSSS SLDCLSEVVGAIQA
Sbjct: 1   MKKDCTDLIRRIALLIHLAEEITNFCSGSGENFEALNDDGSSSSSSSLDCLSEVVGAIQA 60

Query: 61  AKRLLYAALTFSGNDD--ASAASTDGATKKLVYQFQYVTNRLQNALANLPYDHFCISDEV 120
           AKRLLY ALTFS  D+    A ST+ ATKKLV QFQYVT RL+ AL+NLPYDHFC+SDEV
Sbjct: 61  AKRLLYMALTFSVYDEEGCGATSTEEATKKLVLQFQYVTTRLETALSNLPYDHFCVSDEV 120

Query: 121 QEQVDLVRAQLRRAEKKYESMSELANKKLHSQGSVKWKVNNDVTSMSSVDYEVPESQHRP 180
           QEQVDLVRAQLRRA  KYESMS  A KKL ++GSVKW +NNDV S+SSVD    ESQH P
Sbjct: 121 QEQVDLVRAQLRRASNKYESMSNPAEKKLQTRGSVKWMINNDVKSISSVDDGDVESQHHP 180

Query: 181 QNRDYQVGFDPEEPKASDLCMDECSSVVQSDMEDVQATKSQEEVEKSDEIMIPENFLCPI 240
           +N +Y   FD     + + C DE SSVV SDMEDV A+KSQ+EV K  E+ IPENFLCPI
Sbjct: 181 RNCNYPTSFD-----SVNSCFDESSSVVHSDMEDVLASKSQDEVRKPIEVDIPENFLCPI 240

Query: 241 SFELMVDPVIISTGQTYERSNIQRWIDKGNKTCPKTQEQLQALILTPNFLMKQLISEWCQ 300
           SFELM+DPVI STGQTYERSN+Q WID+GN+TCPKTQEQLQ LILTPNFLM++LI+EWC+
Sbjct: 241 SFELMLDPVICSTGQTYERSNVQNWIDRGNRTCPKTQEQLQTLILTPNFLMRKLIAEWCE 300

Query: 301 EHNVKLEEGLTTRKIKKYRSFEDGSRKRVPVKTLVRHLSFGSAHEKKAAVTEIRKLTKSS 360
           EHNVKLEEGLT+RK KKYRSFED  R+ +P+KTLVRHLSFGS  E+K AVTEIRKL+KSS
Sbjct: 301 EHNVKLEEGLTSRKFKKYRSFEDDCRRTLPIKTLVRHLSFGSIQEQKIAVTEIRKLSKSS 360

Query: 361 SEHRVEIAEAGAIPQLVNLLTSEDVLTQENAISCILNLSLHEQNKRLVMLSGAVSYISQV 420
           S+HRVEIAEAGAIPQLVNLLTSED++TQENA+SCILNLSLHEQNKRLVMLSGAVSYIS+V
Sbjct: 361 SDHRVEIAEAGAIPQLVNLLTSEDIVTQENAMSCILNLSLHEQNKRLVMLSGAVSYISKV 420

Query: 421 LKFGSLEARECAAATLYSLSLADENKAVIGASGVIPGLLEILEIGSARGKKDAARALLNL 480
           LK GS+E RECAAAT+YSLSLADENKA+IGASGVIP LLEILEIGS RG+KDAA ALLNL
Sbjct: 421 LKVGSMEGRECAAATIYSLSLADENKAIIGASGVIPDLLEILEIGSPRGQKDAAGALLNL 480

Query: 481 CMYQGNKSRAVKAGIVRPLLKLLSDSDASMVDEALYIMSVLCVHPEAKAAMANANSLLVL 540
           CMYQGNK RA+KAGIVRPLLK+LSD + S+VD+ALYIMSVLC HPEAKAAMANAN+LLVL
Sbjct: 481 CMYQGNKGRALKAGIVRPLLKILSDPNGSLVDDALYIMSVLCGHPEAKAAMANANTLLVL 540

Query: 541 TEVLKTGASRSKENAAAVMFVLCKGDLEKLSWLTKLGATIPLMILAENGTGRARRKATSL 600
           T+VLK G+SRSKENAAAV+  LCKGD EKL WLT+LGA   LM LAENGTGRARRKA SL
Sbjct: 541 TDVLKMGSSRSKENAAAVLLALCKGDWEKLEWLTRLGAMASLMKLAENGTGRARRKAASL 600

Query: 601 LEELRRS 604
           LE+LR+S
Sbjct: 601 LEQLRKS 602

BLAST of Moc04g27320 vs. NCBI nr
Match: XP_038875664.1 (U-box domain-containing protein 11-like isoform X2 [Benincasa hispida])

HSP 1 Score: 878.6 bits (2269), Expect = 3.0e-251
Identity = 470/607 (77.43%), Postives = 524/607 (86.33%), Query Frame = 0

Query: 1   MQKDCTDLIRRIALLIHLAEEIMNFRRG-GDNLAELNESG-SSSSCSLDCLSEVVGAIQA 60
           M+KDCTDLIRRIALLIHLAEEI NF  G G+N   LN+ G SSSS SLDCLSEVVGAIQA
Sbjct: 13  MKKDCTDLIRRIALLIHLAEEITNFCSGSGENFEALNDDGSSSSSSSLDCLSEVVGAIQA 72

Query: 61  AKRLLYAALTFSGNDD--ASAASTDGATKKLVYQFQYVTNRLQNALANLPYDHFCISDEV 120
           AKRLLY ALTFS  D+    A ST+ ATKKLV QFQYVT RL+ AL+NLPYDHFC+SDEV
Sbjct: 73  AKRLLYMALTFSVYDEEGCGATSTEEATKKLVLQFQYVTTRLETALSNLPYDHFCVSDEV 132

Query: 121 QEQVDLVRAQLRRAEKKYESMSELANKKLHSQGSVKWKVNNDVTSMSSVDYEVPESQHRP 180
           QEQVDLVRAQLRRA  KYESMS  A KKL ++GSVKW +NNDV S+SSVD    ESQH P
Sbjct: 133 QEQVDLVRAQLRRASNKYESMSNPAEKKLQTRGSVKWMINNDVKSISSVDDGDVESQHHP 192

Query: 181 QNRDYQVGFDPEEPKASDLCMDECSSVVQSDMEDVQATKSQEEVEKSDEIMIPENFLCPI 240
           +N +Y   FD     + + C DE SSVV SDMEDV A+KSQ+EV K  E+ IPENFLCPI
Sbjct: 193 RNCNYPTSFD-----SVNSCFDESSSVVHSDMEDVLASKSQDEVRKPIEVDIPENFLCPI 252

Query: 241 SFELMVDPVIISTGQTYERSNIQRWIDKGNKTCPKTQEQLQALILTPNFLMKQLISEWCQ 300
           SFELM+DPVI STGQTYERSN+Q WID+GN+TCPKTQEQLQ LILTPNFLM++LI+EWC+
Sbjct: 253 SFELMLDPVICSTGQTYERSNVQNWIDRGNRTCPKTQEQLQTLILTPNFLMRKLIAEWCE 312

Query: 301 EHNVKLEEGLTTRKIKKYRSFEDGSRKRVPVKTLVRHLSFGSAHEKKAAVTEIRKLTKSS 360
           EHNVKLEEGLT+RK KKYRSFED  R+ +P+KTLVRHLSFGS  E+K AVTEIRKL+KSS
Sbjct: 313 EHNVKLEEGLTSRKFKKYRSFEDDCRRTLPIKTLVRHLSFGSIQEQKIAVTEIRKLSKSS 372

Query: 361 SEHRVEIAEAGAIPQLVNLLTSEDVLTQENAISCILNLSLHEQNKRLVMLSGAVSYISQV 420
           S+HRVEIAEAGAIPQLVNLLTSED++TQENA+SCILNLSLHEQNKRLVMLSGAVSYIS+V
Sbjct: 373 SDHRVEIAEAGAIPQLVNLLTSEDIVTQENAMSCILNLSLHEQNKRLVMLSGAVSYISKV 432

Query: 421 LKFGSLEARECAAATLYSLSLADENKAVIGASGVIPGLLEILEIGSARGKKDAARALLNL 480
           LK GS+E RECAAAT+YSLSLADENKA+IGASGVIP LLEILEIGS RG+KDAA ALLNL
Sbjct: 433 LKVGSMEGRECAAATIYSLSLADENKAIIGASGVIPDLLEILEIGSPRGQKDAAGALLNL 492

Query: 481 CMYQGNKSRAVKAGIVRPLLKLLSDSDASMVDEALYIMSVLCVHPEAKAAMANANSLLVL 540
           CMYQGNK RA+KAGIVRPLLK+LSD + S+VD+ALYIMSVLC HPEAKAAMANAN+LLVL
Sbjct: 493 CMYQGNKGRALKAGIVRPLLKILSDPNGSLVDDALYIMSVLCGHPEAKAAMANANTLLVL 552

Query: 541 TEVLKTGASRSKENAAAVMFVLCKGDLEKLSWLTKLGATIPLMILAENGTGRARRKATSL 600
           T+VLK G+SRSKENAAAV+  LCKGD EKL WLT+LGA   LM LAENGTGRARRKA SL
Sbjct: 553 TDVLKMGSSRSKENAAAVLLALCKGDWEKLEWLTRLGAMASLMKLAENGTGRARRKAASL 612

Query: 601 LEELRRS 604
           LE+LR+S
Sbjct: 613 LEQLRKS 614

BLAST of Moc04g27320 vs. NCBI nr
Match: XP_038875663.1 (U-box domain-containing protein 11-like isoform X1 [Benincasa hispida])

HSP 1 Score: 878.6 bits (2269), Expect = 3.0e-251
Identity = 470/607 (77.43%), Postives = 524/607 (86.33%), Query Frame = 0

Query: 1   MQKDCTDLIRRIALLIHLAEEIMNFRRG-GDNLAELNESG-SSSSCSLDCLSEVVGAIQA 60
           M+KDCTDLIRRIALLIHLAEEI NF  G G+N   LN+ G SSSS SLDCLSEVVGAIQA
Sbjct: 40  MKKDCTDLIRRIALLIHLAEEITNFCSGSGENFEALNDDGSSSSSSSLDCLSEVVGAIQA 99

Query: 61  AKRLLYAALTFSGNDD--ASAASTDGATKKLVYQFQYVTNRLQNALANLPYDHFCISDEV 120
           AKRLLY ALTFS  D+    A ST+ ATKKLV QFQYVT RL+ AL+NLPYDHFC+SDEV
Sbjct: 100 AKRLLYMALTFSVYDEEGCGATSTEEATKKLVLQFQYVTTRLETALSNLPYDHFCVSDEV 159

Query: 121 QEQVDLVRAQLRRAEKKYESMSELANKKLHSQGSVKWKVNNDVTSMSSVDYEVPESQHRP 180
           QEQVDLVRAQLRRA  KYESMS  A KKL ++GSVKW +NNDV S+SSVD    ESQH P
Sbjct: 160 QEQVDLVRAQLRRASNKYESMSNPAEKKLQTRGSVKWMINNDVKSISSVDDGDVESQHHP 219

Query: 181 QNRDYQVGFDPEEPKASDLCMDECSSVVQSDMEDVQATKSQEEVEKSDEIMIPENFLCPI 240
           +N +Y   FD     + + C DE SSVV SDMEDV A+KSQ+EV K  E+ IPENFLCPI
Sbjct: 220 RNCNYPTSFD-----SVNSCFDESSSVVHSDMEDVLASKSQDEVRKPIEVDIPENFLCPI 279

Query: 241 SFELMVDPVIISTGQTYERSNIQRWIDKGNKTCPKTQEQLQALILTPNFLMKQLISEWCQ 300
           SFELM+DPVI STGQTYERSN+Q WID+GN+TCPKTQEQLQ LILTPNFLM++LI+EWC+
Sbjct: 280 SFELMLDPVICSTGQTYERSNVQNWIDRGNRTCPKTQEQLQTLILTPNFLMRKLIAEWCE 339

Query: 301 EHNVKLEEGLTTRKIKKYRSFEDGSRKRVPVKTLVRHLSFGSAHEKKAAVTEIRKLTKSS 360
           EHNVKLEEGLT+RK KKYRSFED  R+ +P+KTLVRHLSFGS  E+K AVTEIRKL+KSS
Sbjct: 340 EHNVKLEEGLTSRKFKKYRSFEDDCRRTLPIKTLVRHLSFGSIQEQKIAVTEIRKLSKSS 399

Query: 361 SEHRVEIAEAGAIPQLVNLLTSEDVLTQENAISCILNLSLHEQNKRLVMLSGAVSYISQV 420
           S+HRVEIAEAGAIPQLVNLLTSED++TQENA+SCILNLSLHEQNKRLVMLSGAVSYIS+V
Sbjct: 400 SDHRVEIAEAGAIPQLVNLLTSEDIVTQENAMSCILNLSLHEQNKRLVMLSGAVSYISKV 459

Query: 421 LKFGSLEARECAAATLYSLSLADENKAVIGASGVIPGLLEILEIGSARGKKDAARALLNL 480
           LK GS+E RECAAAT+YSLSLADENKA+IGASGVIP LLEILEIGS RG+KDAA ALLNL
Sbjct: 460 LKVGSMEGRECAAATIYSLSLADENKAIIGASGVIPDLLEILEIGSPRGQKDAAGALLNL 519

Query: 481 CMYQGNKSRAVKAGIVRPLLKLLSDSDASMVDEALYIMSVLCVHPEAKAAMANANSLLVL 540
           CMYQGNK RA+KAGIVRPLLK+LSD + S+VD+ALYIMSVLC HPEAKAAMANAN+LLVL
Sbjct: 520 CMYQGNKGRALKAGIVRPLLKILSDPNGSLVDDALYIMSVLCGHPEAKAAMANANTLLVL 579

Query: 541 TEVLKTGASRSKENAAAVMFVLCKGDLEKLSWLTKLGATIPLMILAENGTGRARRKATSL 600
           T+VLK G+SRSKENAAAV+  LCKGD EKL WLT+LGA   LM LAENGTGRARRKA SL
Sbjct: 580 TDVLKMGSSRSKENAAAVLLALCKGDWEKLEWLTRLGAMASLMKLAENGTGRARRKAASL 639

Query: 601 LEELRRS 604
           LE+LR+S
Sbjct: 640 LEQLRKS 641

BLAST of Moc04g27320 vs. ExPASy Swiss-Prot
Match: Q8GUG9 (U-box domain-containing protein 11 OS=Arabidopsis thaliana OX=3702 GN=PUB11 PE=2 SV=2)

HSP 1 Score: 500.7 bits (1288), Expect = 2.2e-140
Identity = 302/613 (49.27%), Postives = 407/613 (66.39%), Query Frame = 0

Query: 2   QKDCTDLIRRIALLIHLAEEIMNFRRGGDNLAELNESGSSSSCSLDCLSEVVGAIQAAKR 61
           +KDC DL RR+ LL HL EEI +        +   +S +SSS   D  S++V  +QAAKR
Sbjct: 29  KKDCADLTRRVCLLTHLLEEIRD--------STPIDSAASSSSENDWWSDLVVGLQAAKR 88

Query: 62  LLYAALTFSGNDDASAASTDGATKKLVYQFQYVTNRLQNALANLPYDHFCISDEVQEQVD 121
           LL  A  F   D     S+DGA K++ +QFQ VT +L+ AL+NLPYD + ISDEV EQV+
Sbjct: 89  LLSTA-RFQARD-----SSDGAAKRISFQFQCVTWKLEKALSNLPYDLYDISDEVGEQVE 148

Query: 122 LVRAQLRRAEKKYESMSELANKKLHSQGSVKWKVNNDVTSMSSVDYE-VPESQHRPQNRD 181
           L R+QLRRA ++Y S++        S+   +   +N +   +    E V E+ H      
Sbjct: 149 LARSQLRRAMQRYGSLNSNKFSSALSEPMERDGFSNVIKIKAEEKLESVSETLH------ 208

Query: 182 YQVGFDPEEPKASDLCMDECSSV---------VQSDMEDVQATKSQEEVEKSDEIMIPEN 241
               F  EE K S   +   SS+           +D  D    K+ +E +KSD++ IP +
Sbjct: 209 ----FGEEEEKQSSPPLRRSSSISLAYYLSKDADTDRLDKMVNKNTDESKKSDKLTIPVD 268

Query: 242 FLCPISFELMVDPVIISTGQTYERSNIQRWIDKGNKTCPKTQEQLQALILTPNFLMKQLI 301
           FLCP+S ELM DPVI++TGQTYER+ IQRWID GN TCPKTQ++L+   LTPN++++ LI
Sbjct: 269 FLCPVSLELMKDPVIVATGQTYERAYIQRWIDCGNLTCPKTQQKLENFTLTPNYVLRSLI 328

Query: 302 SEWCQEHNVKLEEGLTTRKIKKYRSFEDGSRKRVPVKTLVRHLSFGSAHEKKAAVTEIRK 361
           S WC EHN++   G    + K        S     ++ LV+ LS  S  +++ AV+EIR 
Sbjct: 329 SRWCAEHNIEQPAGYINGRTK-------NSGDMSVIRALVQRLSSRSTEDRRNAVSEIRS 388

Query: 362 LTKSSSEHRVEIAEAGAIPQLVNLLTSEDVLTQENAISCILNLSLHEQNKRLVMLSGAVS 421
           L+K S+++R+ IAEAGAIP LVNLLTSEDV TQENAI+C+LNLS++E NK L+M +GAV+
Sbjct: 389 LSKRSTDNRILIAEAGAIPVLVNLLTSEDVATQENAITCVLNLSIYENNKELIMFAGAVT 448

Query: 422 YISQVLKFGSLEARECAAATLYSLSLADENKAVIGASGVIPGLLEILEIGSARGKKDAAR 481
            I QVL+ G++EARE AAATL+SLSLADENK +IG SG IP L+++LE G+ RGKKDAA 
Sbjct: 449 SIVQVLRAGTMEARENAAATLFSLSLADENKIIIGGSGAIPALVDLLENGTPRGKKDAAT 508

Query: 482 ALLNLCMYQGNKSRAVKAGIVRPLLKLLSDSDA-SMVDEALYIMSVLCVHPEAKAAMANA 541
           AL NLC+Y GNK RAV+AGIV  L+K+LSDS    MVDEAL I+SVL  + +AK+A+  A
Sbjct: 509 ALFNLCIYHGNKGRAVRAGIVTALVKMLSDSTRHRMVDEALTILSVLANNQDAKSAIVKA 568

Query: 542 NSLLVLTEVLKTGASRSKENAAAVMFVLCKGDLEKLSWLTKLGATIPLMILAENGTGRAR 601
           N+L  L  +L+T  +R++ENAAA++  LCK D EKL  + +LGA +PLM L++NGT R +
Sbjct: 569 NTLPALIGILQTDQTRNRENAAAILLSLCKRDTEKLITIGRLGAVVPLMDLSKNGTERGK 610

Query: 602 RKATSLLEELRRS 604
           RKA SLLE LR++
Sbjct: 629 RKAISLLELLRKA 610

BLAST of Moc04g27320 vs. ExPASy Swiss-Prot
Match: Q9C9A6 (U-box domain-containing protein 10 OS=Arabidopsis thaliana OX=3702 GN=PUB10 PE=2 SV=1)

HSP 1 Score: 493.8 bits (1270), Expect = 2.7e-138
Identity = 301/612 (49.18%), Postives = 413/612 (67.48%), Query Frame = 0

Query: 2   QKDCTDLIRRIALLIHLAEEIMNFRRGGDNLAELNESGSSSSCSLDCLSEVVGAIQAAKR 61
           +KDC+DL RR+ LL HL EEI    R     +E + S S +S   D  S++V  +QAAKR
Sbjct: 29  KKDCSDLARRVGLLTHLIEEI----RDSSPPSESDASSSLNSHECDWWSDLVVGLQAAKR 88

Query: 62  LLYAALTFSGNDDASAASTDGATKKLVYQFQYVTNRLQNALANLPYDHFCISDEVQEQVD 121
           LL +A +F   +     S+DGA K++ +QFQ VT +L+ AL +L YD + ISDEV+EQV+
Sbjct: 89  LLSSATSFQARE-----SSDGAAKRISFQFQCVTWKLEKALGDLTYDRYDISDEVREQVE 148

Query: 122 LVRAQLRRAEKKYESMSELANKKLHSQGSVKWKVNNDVTSMSSVDYE---VPESQHRPQN 181
           L R QLRRA ++Y S+    N K  S G +   +  D +S   V  +   +PE+ H    
Sbjct: 149 LARLQLRRAMQRYGSL----NSKKFSSG-LSEPMEKDASSNRKVIEKLESIPETVH--SL 208

Query: 182 RDYQVGFDPEEPKASDLCMDECSSVVQSDMEDVQ----ATKSQEEVEKSDEIMIPENFLC 241
            D +    P   K+S + +   +  +  D +D +     T++ ++ +KSD + IPE+FLC
Sbjct: 209 SDEKKFESPPPWKSSSVSL---AFFLSKDGDDERLEKAVTENSDDSQKSDNLTIPEDFLC 268

Query: 242 PISFELMVDPVIISTGQTYERSNIQRWIDKGNKTCPKTQEQLQALILTPNFLMKQLISEW 301
           PIS ELM DP I+STGQTYERS IQRWID GN +CPKTQ++L+   LTPN++++ LIS+W
Sbjct: 269 PISLELMKDPAIVSTGQTYERSFIQRWIDCGNLSCPKTQQKLENFTLTPNYVLRSLISQW 328

Query: 302 CQEHNVKLEEGLTTRKIKKY-RSFEDGSRKRVPVKTLVRHLSFGSAHEKKAAVTEIRKLT 361
           C +HN++   G    + K    SF D S     ++ LV  LS  S  +++ AV+EIR L+
Sbjct: 329 CTKHNIEQPGGYMNGRTKNSDGSFRDLSGDMSAIRALVCKLSSQSIEDRRTAVSEIRSLS 388

Query: 362 KSSSEHRVEIAEAGAIPQLVNLLTSE-DVLTQENAISCILNLSLHEQNKRLVMLSGAVSY 421
           K S+++R+ IAEAGAIP LV LLTS+ D  TQENA++CILNLS++E NK L+ML+GAV+ 
Sbjct: 389 KRSTDNRILIAEAGAIPVLVKLLTSDGDTETQENAVTCILNLSIYEHNKELIMLAGAVTS 448

Query: 422 ISQVLKFGSLEARECAAATLYSLSLADENKAVIGASGVIPGLLEILEIGSARGKKDAARA 481
           I  VL+ GS+EARE AAATL+SLSLADENK +IGASG I  L+++L+ GS RGKKDAA A
Sbjct: 449 IVLVLRAGSMEARENAAATLFSLSLADENKIIIGASGAIMALVDLLQYGSVRGKKDAATA 508

Query: 482 LLNLCMYQGNKSRAVKAGIVRPLLKLLSDSDAS-MVDEALYIMSVLCVHPEAKAAMANAN 541
           L NLC+YQGNK RAV+AGIV+PL+K+L+DS +  M DEAL I+SVL  +  AK A+  AN
Sbjct: 509 LFNLCIYQGNKGRAVRAGIVKPLVKMLTDSSSERMADEALTILSVLASNQVAKTAILRAN 568

Query: 542 SLLVLTEVLKTGASRSKENAAAVMFVLCKGDLEKLSWLTKLGATIPLMILAENGTGRARR 601
           ++  L + L+    R++ENAAA++  LCK D EKL  + +LGA +PLM L+ +GT RA+R
Sbjct: 569 AIPPLIDCLQKDQPRNRENAAAILLCLCKRDTEKLISIGRLGAVVPLMELSRDGTERAKR 621

Query: 602 KATSLLEELRRS 604
           KA SLLE LR+S
Sbjct: 629 KANSLLELLRKS 621

BLAST of Moc04g27320 vs. ExPASy Swiss-Prot
Match: Q8VZ40 (U-box domain-containing protein 14 OS=Arabidopsis thaliana OX=3702 GN=PUB14 PE=1 SV=1)

HSP 1 Score: 388.3 bits (996), Expect = 1.6e-106
Identity = 244/613 (39.80%), Postives = 378/613 (61.66%), Query Frame = 0

Query: 7   DLIRRIALLIHLAEEI--MNFRRGGDNLAELNESGSSSSCSLDCLSEVVGAIQAAKRLLY 66
           DL+RRI LL    EE+  +N     D +        +   +LD   E+  ++    +L  
Sbjct: 39  DLVRRITLLSPFFEELIDVNVELKKDQITGF----EAMRIALDSSLELFRSVNGGSKL-- 98

Query: 67  AALTFSGNDDASAASTDGATKKLVYQFQYVTNRLQNALANLPYDHFCISDEVQEQVDLVR 126
               F   D  S          LV +F+ +T  ++ AL+ +PY+   +S+EV+EQV L+ 
Sbjct: 99  ----FQLFDRDS----------LVEKFRDMTVEIEAALSQIPYEKIEVSEEVREQVQLLH 158

Query: 127 AQLRRAEKKYESMS-------ELANKKLHSQGSVKWKVNNDVTSMSSVDYEVPESQHRPQ 186
            Q +RA++++E           +A   +     +  +++ ++  ++++D E+ +  H   
Sbjct: 159 FQFKRAKERWEESDLQLSHDLAMAENVMDPDPIILKRLSQEL-QLTTID-ELKKESH--A 218

Query: 187 NRDYQVGFDPEEPKASDLCMDECSSVVQSDMEDVQATKSQEEVEKSDEI-------MIPE 246
             +Y + +D +     D C +  SS++++ ++ V    S  +      I       +IPE
Sbjct: 219 IHEYFLSYDGD----PDDCFERMSSLLKNLVDFVTMESSDPDPSTGSRIVSRHRSPVIPE 278

Query: 247 NFLCPISFELMVDPVIISTGQTYERSNIQRWIDKGNKTCPKTQEQLQALILTPNFLMKQL 306
            F CPIS ELM DPVI+STGQTYERS+IQ+W+D G+KTCPK+QE L    LTPN+++K L
Sbjct: 279 YFRCPISLELMKDPVIVSTGQTYERSSIQKWLDAGHKTCPKSQETLLHAGLTPNYVLKSL 338

Query: 307 ISEWCQEHNVKLEEGLTTRKIKKYRSFEDGSRKRVPVKTLVRHLSFGSAHEKKAAVTEIR 366
           I+ WC+ + ++L +   + +  K          R  V +L+  L+ G+  +++AA  E+R
Sbjct: 339 IALWCESNGIELPQNQGSCRTTKIGGSSSSDCDRTFVLSLLEKLANGTTEQQRAAAGELR 398

Query: 367 KLTKSSSEHRVEIAEAGAIPQLVNLLTSEDVLTQENAISCILNLSLHEQNKRLVMLSGAV 426
            L K + ++RV IAEAGAIP LV LL+S D  TQE++++ +LNLS++E NK  ++ +GA+
Sbjct: 399 LLAKRNVDNRVCIAEAGAIPLLVELLSSPDPRTQEHSVTALLNLSINEGNKGAIVDAGAI 458

Query: 427 SYISQVLKFGSLEARECAAATLYSLSLADENKAVIGASGVIPGLLEILEIGSARGKKDAA 486
           + I +VLK GS+EARE AAATL+SLS+ DENK  IGA+G I  L+ +LE G+ RGKKDAA
Sbjct: 459 TDIVEVLKNGSMEARENAAATLFSLSVIDENKVAIGAAGAIQALISLLEEGTRRGKKDAA 518

Query: 487 RALLNLCMYQGNKSRAVKAGIVRPLLKLLSDSDASMVDEALYIMSVLCVHPEAKAAMANA 546
            A+ NLC+YQGNKSRAVK GIV PL +LL D+   MVDEAL I+++L  + E K A+A A
Sbjct: 519 TAIFNLCIYQGNKSRAVKGGIVDPLTRLLKDAGGGMVDEALAILAILSTNQEGKTAIAEA 578

Query: 547 NSLLVLTEVLKTGASRSKENAAAVMFVLCKGDLEKLSWLTKLGATIPLMILAENGTGRAR 604
            S+ VL E+++TG+ R++ENAAA+++ LC G++E+L+   ++GA + L  L ENGT RA+
Sbjct: 579 ESIPVLVEIIRTGSPRNRENAAAILWYLCIGNIERLNVAREVGADVALKELTENGTDRAK 623

BLAST of Moc04g27320 vs. ExPASy Swiss-Prot
Match: Q9SNC6 (U-box domain-containing protein 13 OS=Arabidopsis thaliana OX=3702 GN=PUB13 PE=1 SV=1)

HSP 1 Score: 367.1 bits (941), Expect = 3.9e-100
Identity = 247/635 (38.90%), Postives = 375/635 (59.06%), Query Frame = 0

Query: 1   MQKDCTDLIRRIALLIHLAEEIMNFRRGGDNLAELNESGSSSSCSLDCLSEVVGAIQAAK 60
           ++K C +L RR+ LL+ + EEI           E NE  S    +L  L  +  A+ +AK
Sbjct: 29  VKKLCYNLARRLKLLVPMFEEI----------RESNEPISED--TLKTLMNLKEAMCSAK 88

Query: 61  RLLYAALTFSGNDDASAASTDGATKKLVYQFQYVTNRLQNALANLPYDHFCISDEVQEQV 120
              Y      G+        +  T KL+     V+ +L+ +L+ +PY+   ISDEV+EQV
Sbjct: 89  D--YLKFCSQGSKIYLVMEREQVTSKLM----EVSVKLEQSLSQIPYEELDISDEVREQV 148

Query: 121 DLVRAQLRRA--------EKKYESMSELANKKLHSQGSVKWKVNNDVTSMSSVDYEVPES 180
           +LV +Q RRA        ++ YE +  L NK            ++DV +   V   V + 
Sbjct: 149 ELVLSQFRRAKGRVDVSDDELYEDLQSLCNK------------SSDVDAYQPVLERVAKK 208

Query: 181 QHRPQNRDY-QVGFDPEEPKASD-----LCMDECSSVVQSDMEDVQATKSQEEVEK---- 240
            H  +  D  Q      E  AS        ++E + V++   + VQ      E +K    
Sbjct: 209 LHLMEIPDLAQESVALHEMVASSGGDVGENIEEMAMVLKMIKDFVQTEDDNGEEQKVGVN 268

Query: 241 -----------SDEI-MIPENFLCPISFELMVDPVIISTGQTYERSNIQRWIDKGNKTCP 300
                      S +I +IP++F CPIS E+M DPVI+S+GQTYER+ I++WI+ G+ TCP
Sbjct: 269 SRSNGQTSTAASQKIPVIPDDFRCPISLEMMRDPVIVSSGQTYERTCIEKWIEGGHSTCP 328

Query: 301 KTQEQLQALILTPNFLMKQLISEWCQEHNV---KLEEGLTTRKIKKYRSFEDGSRKRVPV 360
           KTQ+ L +  LTPN++++ LI++WC+ +++   K    L  RK+  + S  + ++    +
Sbjct: 329 KTQQALTSTTLTPNYVLRSLIAQWCEANDIEPPKPPSSLRPRKVSSFSSPAEANK----I 388

Query: 361 KTLVRHLSFGSAHEKKAAVTEIRKLTKSSSEHRVEIAEAGAIPQLVNLLTSEDVLTQENA 420
           + L+  L++G+  ++++A  EIR L K ++++RV IAEAGAIP LV LL++ D   QE++
Sbjct: 389 EDLMWRLAYGNPEDQRSAAGEIRLLAKRNADNRVAIAEAGAIPLLVGLLSTPDSRIQEHS 448

Query: 421 ISCILNLSLHEQNKRLVMLSGAVSYISQVLKFGSLEARECAAATLYSLSLADENKAVIGA 480
           ++ +LNLS+ E NK  ++ +GA+  I QVLK GS+EARE AAATL+SLS+ DENK  IGA
Sbjct: 449 VTALLNLSICENNKGAIVSAGAIPGIVQVLKKGSMEARENAAATLFSLSVIDENKVTIGA 508

Query: 481 SGVIPGLLEILEIGSARGKKDAARALLNLCMYQGNKSRAVKAGIVRPLLKLLSDSDASMV 540
            G IP L+ +L  G+ RGKKDAA AL NLC+YQGNK +A++AG++  L +LL++  + MV
Sbjct: 509 LGAIPPLVVLLNEGTQRGKKDAATALFNLCIYQGNKGKAIRAGVIPTLTRLLTEPGSGMV 568

Query: 541 DEALYIMSVLCVHPEAKAAMANANSLLVLTEVLKTGASRSKENAAAVMFVLCKGDLEKLS 600
           DEAL I+++L  HPE KA + +++++  L E ++TG+ R++ENAAAV+  LC GD + L 
Sbjct: 569 DEALAILAILSSHPEGKAIIGSSDAVPSLVEFIRTGSPRNRENAAAVLVHLCSGDPQHLV 628

Query: 601 WLTKLGATIPLMILAENGTGRARRKATSLLEELRR 603
              KLG   PL+ LA NGT R +RKA  LLE + R
Sbjct: 629 EAQKLGLMGPLIDLAGNGTDRGKRKAAQLLERISR 629

BLAST of Moc04g27320 vs. ExPASy Swiss-Prot
Match: Q5VRH9 (U-box domain-containing protein 12 OS=Oryza sativa subsp. japonica OX=39947 GN=PUB12 PE=2 SV=1)

HSP 1 Score: 366.7 bits (940), Expect = 5.0e-100
Identity = 247/620 (39.84%), Postives = 353/620 (56.94%), Query Frame = 0

Query: 1   MQKDCTDLIRRIALLIHLAEEIMNFRRGGDNLAELNESGSSSSCSLDCLSEVVGAIQAAK 60
           +++ C DL RR+ LL  L + +                 SSSS S   L++ +G   AA+
Sbjct: 19  LRRPCADLSRRVRLLAPLLDHL---------------PASSSSSSSTPLADALG---AAR 78

Query: 61  RLLYAALTFSGNDDASAASTDGATKKLVYQFQYVTNRLQNALANLPYDHFCISDEVQEQV 120
            LL      S  D A            + +F  V  ++  AL  LPY+ F +  EVQEQV
Sbjct: 79  DLLRKTRDGSKIDQAMRGDA------FLDEFAGVNRQIHLALDALPYNTFHMPQEVQEQV 138

Query: 121 DLVRAQLRRAEKKYESMSELANKKLHSQGSVKWKVN---NDVTSMSSVDYE-----VPES 180
            LV +Q +RA  + +      +  L       W +    +D   ++ + ++     + + 
Sbjct: 139 ALVHSQFQRASTRTDPPDTQLSMDL------AWALTDNPSDPALLTRISHKLQLHTMADM 198

Query: 181 QHRPQNRDYQVGFDPEEPKASDLCMDECSS---------VVQSDMEDVQATKSQEEVEKS 240
           ++        V     EP   D C+D+ SS         V +    D   T+S     +S
Sbjct: 199 KNESIALHNMVISTAGEP---DGCVDQMSSLLKKLKDCVVTEDHANDALTTRSASIKHRS 258

Query: 241 DEIMIPENFLCPISFELMVDPVIISTGQTYERSNIQRWIDKGNKTCPKTQEQLQALILTP 300
              +IP+ F CPIS ELM DPVI+S+GQTYERS IQ+W+D G+KTCPKTQ+ L    LTP
Sbjct: 259 P--IIPDEFRCPISLELMQDPVIVSSGQTYERSCIQKWLDSGHKTCPKTQQPLSHTSLTP 318

Query: 301 NFLMKQLISEWCQEHNVKLEEGLTTRKIKKYRSFEDGSRKRVPVKTLVRHLSFGSAHEKK 360
           NF++K LIS+WC+ + ++L +     + KK     D       + +L+  L  G+  E++
Sbjct: 319 NFVLKSLISQWCEANGIELPKNKQNSRDKKAAKSSD--YDHAGLVSLMNRLRSGNQDEQR 378

Query: 361 AAVTEIRKLTKSSSEHRVEIAEAGAIPQLVNLLTSEDVLTQENAISCILNLSLHEQNKRL 420
           AA  EIR L K +  +R+ IAEAGAIP LVNLL+S D  TQE+A++ +LNLS+HE NK  
Sbjct: 379 AAAGEIRLLAKRNVNNRICIAEAGAIPLLVNLLSSSDPRTQEHAVTALLNLSIHENNKAS 438

Query: 421 VMLSGAVSYISQVLKFGSLEARECAAATLYSLSLADENKAVIGASGVIPGLLEILEIGSA 480
           ++ S A+  I +VLK GS+E RE AAATL+SLS+ DENK  IGA+G IP L+ +L  GS 
Sbjct: 439 IVDSHAIPKIVEVLKTGSMETRENAAATLFSLSVVDENKVTIGAAGAIPPLINLLCDGSP 498

Query: 481 RGKKDAARALLNLCMYQGNKSRAVKAGIVRPLLKLLSDSDASMVDEALYIMSVLCVHPEA 540
           RGKKDAA A+ NLC+YQGNK RAVKAGIV  L+  L D    M+DEAL ++S+L  +PE 
Sbjct: 499 RGKKDAATAIFNLCIYQGNKVRAVKAGIVIHLMNFLVDPTGGMIDEALSLLSILAGNPEG 558

Query: 541 KAAMANANSLLVLTEVLKTGASRSKENAAAVMFVLCKGDLEKLSWLTKLGATIPLMILAE 600
           K  +A +  +  L EV+KTG+ R++ENAAA++++LC  D E+       G    L  L+E
Sbjct: 559 KIVIARSEPIPPLVEVIKTGSPRNRENAAAILWLLCSADTEQTLAAKAAGVEDALKELSE 601

Query: 601 NGTGRARRKATSLLEELRRS 604
            GT RA+RKA+S+LE + ++
Sbjct: 619 TGTDRAKRKASSILELMHQA 601

BLAST of Moc04g27320 vs. ExPASy TrEMBL
Match: A0A6J1DFQ4 (RING-type E3 ubiquitin transferase OS=Momordica charantia OX=3673 GN=LOC111020079 PE=4 SV=1)

HSP 1 Score: 1145.2 bits (2961), Expect = 0.0e+00
Identity = 603/603 (100.00%), Postives = 603/603 (100.00%), Query Frame = 0

Query: 1   MQKDCTDLIRRIALLIHLAEEIMNFRRGGDNLAELNESGSSSSCSLDCLSEVVGAIQAAK 60
           MQKDCTDLIRRIALLIHLAEEIMNFRRGGDNLAELNESGSSSSCSLDCLSEVVGAIQAAK
Sbjct: 40  MQKDCTDLIRRIALLIHLAEEIMNFRRGGDNLAELNESGSSSSCSLDCLSEVVGAIQAAK 99

Query: 61  RLLYAALTFSGNDDASAASTDGATKKLVYQFQYVTNRLQNALANLPYDHFCISDEVQEQV 120
           RLLYAALTFSGNDDASAASTDGATKKLVYQFQYVTNRLQNALANLPYDHFCISDEVQEQV
Sbjct: 100 RLLYAALTFSGNDDASAASTDGATKKLVYQFQYVTNRLQNALANLPYDHFCISDEVQEQV 159

Query: 121 DLVRAQLRRAEKKYESMSELANKKLHSQGSVKWKVNNDVTSMSSVDYEVPESQHRPQNRD 180
           DLVRAQLRRAEKKYESMSELANKKLHSQGSVKWKVNNDVTSMSSVDYEVPESQHRPQNRD
Sbjct: 160 DLVRAQLRRAEKKYESMSELANKKLHSQGSVKWKVNNDVTSMSSVDYEVPESQHRPQNRD 219

Query: 181 YQVGFDPEEPKASDLCMDECSSVVQSDMEDVQATKSQEEVEKSDEIMIPENFLCPISFEL 240
           YQVGFDPEEPKASDLCMDECSSVVQSDMEDVQATKSQEEVEKSDEIMIPENFLCPISFEL
Sbjct: 220 YQVGFDPEEPKASDLCMDECSSVVQSDMEDVQATKSQEEVEKSDEIMIPENFLCPISFEL 279

Query: 241 MVDPVIISTGQTYERSNIQRWIDKGNKTCPKTQEQLQALILTPNFLMKQLISEWCQEHNV 300
           MVDPVIISTGQTYERSNIQRWIDKGNKTCPKTQEQLQALILTPNFLMKQLISEWCQEHNV
Sbjct: 280 MVDPVIISTGQTYERSNIQRWIDKGNKTCPKTQEQLQALILTPNFLMKQLISEWCQEHNV 339

Query: 301 KLEEGLTTRKIKKYRSFEDGSRKRVPVKTLVRHLSFGSAHEKKAAVTEIRKLTKSSSEHR 360
           KLEEGLTTRKIKKYRSFEDGSRKRVPVKTLVRHLSFGSAHEKKAAVTEIRKLTKSSSEHR
Sbjct: 340 KLEEGLTTRKIKKYRSFEDGSRKRVPVKTLVRHLSFGSAHEKKAAVTEIRKLTKSSSEHR 399

Query: 361 VEIAEAGAIPQLVNLLTSEDVLTQENAISCILNLSLHEQNKRLVMLSGAVSYISQVLKFG 420
           VEIAEAGAIPQLVNLLTSEDVLTQENAISCILNLSLHEQNKRLVMLSGAVSYISQVLKFG
Sbjct: 400 VEIAEAGAIPQLVNLLTSEDVLTQENAISCILNLSLHEQNKRLVMLSGAVSYISQVLKFG 459

Query: 421 SLEARECAAATLYSLSLADENKAVIGASGVIPGLLEILEIGSARGKKDAARALLNLCMYQ 480
           SLEARECAAATLYSLSLADENKAVIGASGVIPGLLEILEIGSARGKKDAARALLNLCMYQ
Sbjct: 460 SLEARECAAATLYSLSLADENKAVIGASGVIPGLLEILEIGSARGKKDAARALLNLCMYQ 519

Query: 481 GNKSRAVKAGIVRPLLKLLSDSDASMVDEALYIMSVLCVHPEAKAAMANANSLLVLTEVL 540
           GNKSRAVKAGIVRPLLKLLSDSDASMVDEALYIMSVLCVHPEAKAAMANANSLLVLTEVL
Sbjct: 520 GNKSRAVKAGIVRPLLKLLSDSDASMVDEALYIMSVLCVHPEAKAAMANANSLLVLTEVL 579

Query: 541 KTGASRSKENAAAVMFVLCKGDLEKLSWLTKLGATIPLMILAENGTGRARRKATSLLEEL 600
           KTGASRSKENAAAVMFVLCKGDLEKLSWLTKLGATIPLMILAENGTGRARRKATSLLEEL
Sbjct: 580 KTGASRSKENAAAVMFVLCKGDLEKLSWLTKLGATIPLMILAENGTGRARRKATSLLEEL 639

Query: 601 RRS 604
           RRS
Sbjct: 640 RRS 642

BLAST of Moc04g27320 vs. ExPASy TrEMBL
Match: A0A6J1DDN2 (RING-type E3 ubiquitin transferase OS=Momordica charantia OX=3673 GN=LOC111020079 PE=4 SV=1)

HSP 1 Score: 1145.2 bits (2961), Expect = 0.0e+00
Identity = 603/603 (100.00%), Postives = 603/603 (100.00%), Query Frame = 0

Query: 1   MQKDCTDLIRRIALLIHLAEEIMNFRRGGDNLAELNESGSSSSCSLDCLSEVVGAIQAAK 60
           MQKDCTDLIRRIALLIHLAEEIMNFRRGGDNLAELNESGSSSSCSLDCLSEVVGAIQAAK
Sbjct: 1   MQKDCTDLIRRIALLIHLAEEIMNFRRGGDNLAELNESGSSSSCSLDCLSEVVGAIQAAK 60

Query: 61  RLLYAALTFSGNDDASAASTDGATKKLVYQFQYVTNRLQNALANLPYDHFCISDEVQEQV 120
           RLLYAALTFSGNDDASAASTDGATKKLVYQFQYVTNRLQNALANLPYDHFCISDEVQEQV
Sbjct: 61  RLLYAALTFSGNDDASAASTDGATKKLVYQFQYVTNRLQNALANLPYDHFCISDEVQEQV 120

Query: 121 DLVRAQLRRAEKKYESMSELANKKLHSQGSVKWKVNNDVTSMSSVDYEVPESQHRPQNRD 180
           DLVRAQLRRAEKKYESMSELANKKLHSQGSVKWKVNNDVTSMSSVDYEVPESQHRPQNRD
Sbjct: 121 DLVRAQLRRAEKKYESMSELANKKLHSQGSVKWKVNNDVTSMSSVDYEVPESQHRPQNRD 180

Query: 181 YQVGFDPEEPKASDLCMDECSSVVQSDMEDVQATKSQEEVEKSDEIMIPENFLCPISFEL 240
           YQVGFDPEEPKASDLCMDECSSVVQSDMEDVQATKSQEEVEKSDEIMIPENFLCPISFEL
Sbjct: 181 YQVGFDPEEPKASDLCMDECSSVVQSDMEDVQATKSQEEVEKSDEIMIPENFLCPISFEL 240

Query: 241 MVDPVIISTGQTYERSNIQRWIDKGNKTCPKTQEQLQALILTPNFLMKQLISEWCQEHNV 300
           MVDPVIISTGQTYERSNIQRWIDKGNKTCPKTQEQLQALILTPNFLMKQLISEWCQEHNV
Sbjct: 241 MVDPVIISTGQTYERSNIQRWIDKGNKTCPKTQEQLQALILTPNFLMKQLISEWCQEHNV 300

Query: 301 KLEEGLTTRKIKKYRSFEDGSRKRVPVKTLVRHLSFGSAHEKKAAVTEIRKLTKSSSEHR 360
           KLEEGLTTRKIKKYRSFEDGSRKRVPVKTLVRHLSFGSAHEKKAAVTEIRKLTKSSSEHR
Sbjct: 301 KLEEGLTTRKIKKYRSFEDGSRKRVPVKTLVRHLSFGSAHEKKAAVTEIRKLTKSSSEHR 360

Query: 361 VEIAEAGAIPQLVNLLTSEDVLTQENAISCILNLSLHEQNKRLVMLSGAVSYISQVLKFG 420
           VEIAEAGAIPQLVNLLTSEDVLTQENAISCILNLSLHEQNKRLVMLSGAVSYISQVLKFG
Sbjct: 361 VEIAEAGAIPQLVNLLTSEDVLTQENAISCILNLSLHEQNKRLVMLSGAVSYISQVLKFG 420

Query: 421 SLEARECAAATLYSLSLADENKAVIGASGVIPGLLEILEIGSARGKKDAARALLNLCMYQ 480
           SLEARECAAATLYSLSLADENKAVIGASGVIPGLLEILEIGSARGKKDAARALLNLCMYQ
Sbjct: 421 SLEARECAAATLYSLSLADENKAVIGASGVIPGLLEILEIGSARGKKDAARALLNLCMYQ 480

Query: 481 GNKSRAVKAGIVRPLLKLLSDSDASMVDEALYIMSVLCVHPEAKAAMANANSLLVLTEVL 540
           GNKSRAVKAGIVRPLLKLLSDSDASMVDEALYIMSVLCVHPEAKAAMANANSLLVLTEVL
Sbjct: 481 GNKSRAVKAGIVRPLLKLLSDSDASMVDEALYIMSVLCVHPEAKAAMANANSLLVLTEVL 540

Query: 541 KTGASRSKENAAAVMFVLCKGDLEKLSWLTKLGATIPLMILAENGTGRARRKATSLLEEL 600
           KTGASRSKENAAAVMFVLCKGDLEKLSWLTKLGATIPLMILAENGTGRARRKATSLLEEL
Sbjct: 541 KTGASRSKENAAAVMFVLCKGDLEKLSWLTKLGATIPLMILAENGTGRARRKATSLLEEL 600

Query: 601 RRS 604
           RRS
Sbjct: 601 RRS 603

BLAST of Moc04g27320 vs. ExPASy TrEMBL
Match: A0A1S3CSU4 (RING-type E3 ubiquitin transferase OS=Cucumis melo OX=3656 GN=LOC103504572 PE=4 SV=1)

HSP 1 Score: 849.7 bits (2194), Expect = 7.3e-243
Identity = 455/608 (74.84%), Postives = 521/608 (85.69%), Query Frame = 0

Query: 1   MQKDCTDLIRRIALLIHLAEEIMNFRRG-GDNLAELNESGSSSSCS--LDCLSEVVGAIQ 60
           M+KDCTDLIRRIALLIHLAEEI NF  G GDN  + N+ GSS+S S  LDCLSEVVGAIQ
Sbjct: 40  MKKDCTDLIRRIALLIHLAEEITNFCSGSGDNFEKSNDDGSSTSLSSWLDCLSEVVGAIQ 99

Query: 61  AAKRLLYAALTFSGNDDASAA-STDGATKKLVYQFQYVTNRLQNALANLPYDHFCISDEV 120
           AAKRLLY ALTFS  D+   A ST+  TKKLV QF++VT RL+ AL+NLPYD FC+SDEV
Sbjct: 100 AAKRLLYTALTFSAYDEEGCAPSTEEGTKKLVLQFKHVTTRLETALSNLPYDQFCVSDEV 159

Query: 121 QEQVDLVRAQLRRAEKKYESMSELANKKLHSQGSVKWKVNNDVTSMSSVDYEVPESQHRP 180
           QEQVDLVRAQLRRA  KYESMS  A KKL ++ SVKW +NN+V SM+SVD +  ESQHRP
Sbjct: 160 QEQVDLVRAQLRRASHKYESMSNPAEKKLQARSSVKWMINNEVRSMASVD-DGDESQHRP 219

Query: 181 QNRDYQVGFDPEEPKASDLCMDECSSVVQSDMEDVQATKSQEEVEKSDEIMIPENFLCPI 240
           +NRD+    D     + + C DECSSVV SD EDV A++SQ+EV+K  EI IPENFLCPI
Sbjct: 220 RNRDHLASLD-----SVNSCFDECSSVVHSDTEDVVASRSQDEVKKPLEIEIPENFLCPI 279

Query: 241 SFELMVDPVIISTGQTYERSNIQRWIDKGNKTCPKTQEQLQALILTPNFLMKQLISEWCQ 300
           S+ELM+DPVIISTGQTYERSNIQ+WID+GN+ CPKTQEQLQALILTPNF+M++LI EWC+
Sbjct: 280 SYELMLDPVIISTGQTYERSNIQKWIDRGNRICPKTQEQLQALILTPNFIMRKLIYEWCE 339

Query: 301 EHNVKLEEGLTTRKIKKYRSFEDGSRKR-VPVKTLVRHLSFGSAHEKKAAVTEIRKLTKS 360
           EHNVKLEEGLT  K+KK RS ED  R+  +P+KTLVRHLSFGS  E+K AVTEIR+L+KS
Sbjct: 340 EHNVKLEEGLTNGKLKKCRSSEDDCRRTPLPIKTLVRHLSFGSVQEQKIAVTEIRQLSKS 399

Query: 361 SSEHRVEIAEAGAIPQLVNLLTSEDVLTQENAISCILNLSLHEQNKRLVMLSGAVSYISQ 420
           SSEHRVEIAEAGAIPQLVNLL+S+DV+TQENAISCILNLSLHEQNKRL+MLSGAVSYISQ
Sbjct: 400 SSEHRVEIAEAGAIPQLVNLLSSKDVITQENAISCILNLSLHEQNKRLIMLSGAVSYISQ 459

Query: 421 VLKFGSLEARECAAATLYSLSLADENKAVIGASGVIPGLLEILEIGSARGKKDAARALLN 480
           VLK GS+E RECAAAT+YSLSLADENKA+IGASGVIP LLEIL+IG+ RG+KDAA ALLN
Sbjct: 460 VLKVGSMEGRECAAATIYSLSLADENKAIIGASGVIPDLLEILDIGTPRGQKDAAGALLN 519

Query: 481 LCMYQGNKSRAVKAGIVRPLLKLLSDSDASMVDEALYIMSVLCVHPEAKAAMANANSLLV 540
           LCMYQGNK RA+ AGIV+PLLK+LSDS+ S+VD+ALYIMS+LC HP+AKAAM NANSLLV
Sbjct: 520 LCMYQGNKGRALNAGIVKPLLKMLSDSNGSLVDDALYIMSILCGHPDAKAAMGNANSLLV 579

Query: 541 LTEVLKTGASRSKENAAAVMFVLCKGDLEKLSWLTKLGATIPLMILAENGTGRARRKATS 600
           LT+VLKTG+ RSKENAAAV+   CKGD EKL WLT+LGA  PLM LAENGTGRARRKA S
Sbjct: 580 LTDVLKTGSPRSKENAAAVLLAFCKGDREKLEWLTRLGAMAPLMKLAENGTGRARRKAAS 639

Query: 601 LLEELRRS 604
           LL++LR+S
Sbjct: 640 LLDQLRKS 641

BLAST of Moc04g27320 vs. ExPASy TrEMBL
Match: A0A6J1K2B7 (RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111489474 PE=4 SV=1)

HSP 1 Score: 830.9 bits (2145), Expect = 3.5e-237
Identity = 446/607 (73.48%), Postives = 513/607 (84.51%), Query Frame = 0

Query: 1   MQKDCTDLIRRIALLIHLAEEIMNFRRG-GDNLAELNESGSSSSCS-LDCLSEVVGAIQA 60
           M+KDCTDLIRRIALLIHLAEEI NF  G  D    L +S SSSS S LDCLSEV+GAIQA
Sbjct: 73  MKKDCTDLIRRIALLIHLAEEITNFCGGFRDKFEALTDSISSSSSSCLDCLSEVIGAIQA 132

Query: 61  AKRLLYAALTFS--GNDDASAASTDGATKKLVYQFQYVTNRLQNALANLPYDHFCISDEV 120
           AKRLLY+A+TFS   +D+ S  ST+GATKKLV QF YVT+RL+ AL+NLPYDHFC++DEV
Sbjct: 133 AKRLLYSAMTFSVYDDDEVSLTSTEGATKKLVLQFHYVTSRLETALSNLPYDHFCVADEV 192

Query: 121 QEQVDLVRAQLRRAEKKYESMSELANKKLHSQGSVKWKVNNDVTSMSSVDYEVPESQHRP 180
           QEQVDLVRAQL RA K YESMS+  +KKL + GSVK  + +DV +MSSVD   P+SQH P
Sbjct: 193 QEQVDLVRAQLMRASKNYESMSDPTHKKLEATGSVKAMIFHDVKTMSSVDNGDPDSQHCP 252

Query: 181 QNRDYQVGFDPEEPKASDLCMDECSSVVQSDMEDVQATKSQEEVEKSDEIMIPENFLCPI 240
            NRD  + FD +E K S+ C +ECSS V S+MEDV + KSQ+EV K D I IPENFLC I
Sbjct: 253 PNRDDLIRFDSDEIKNSNPCFNECSS-VHSEMEDVLSIKSQDEVGKLDVIEIPENFLCSI 312

Query: 241 SFELMVDPVIISTGQTYERSNIQRWIDKGNKTCPKTQEQLQALILTPNFLMKQLISEWCQ 300
           S ELM+DPVI+STGQTYER NIQ+WID+GN TCPKTQEQLQ+LILTPNF M+ LISEWC 
Sbjct: 313 SLELMIDPVIVSTGQTYERYNIQKWIDRGNTTCPKTQEQLQSLILTPNFAMRNLISEWCG 372

Query: 301 EHNVKLEEGLTTRKIKKYRSFEDGSRKRVPVKTLVRHLSFGSAHEKKAAVTEIRKLTKSS 360
           EHNV LE+GLT RK+KKYRSFEDG R+ +P+KTLVRHLSFGS  E+KAAVTEIR+L+KSS
Sbjct: 373 EHNVNLEKGLTNRKLKKYRSFEDGCRRTLPIKTLVRHLSFGSVQEQKAAVTEIRQLSKSS 432

Query: 361 SEHRVEIAEAGAIPQLVNLLTSEDVLTQENAISCILNLSLHEQNKRLVMLSGAVSYISQV 420
           S+HRVEIA+AGAIPQLV LLTSEDV TQENAISCILNLSLHE NKRL+ML GA SYISQV
Sbjct: 433 SDHRVEIAKAGAIPQLVILLTSEDVATQENAISCILNLSLHEPNKRLIMLHGAFSYISQV 492

Query: 421 LKFGSLEARECAAATLYSLSLADENKAVIGASGVIPGLLEILEIGSARGKKDAARALLNL 480
           LKFGS+E RECAA T+YSLSLADENKA+IGASGVIP L+EIL+IGS RG+KDAA ALLNL
Sbjct: 493 LKFGSMEGRECAAGTIYSLSLADENKAMIGASGVIPDLIEILQIGSPRGQKDAAGALLNL 552

Query: 481 CMYQGNKSRAVKAGIVRPLLKLLSDSDASMVDEALYIMSVLCVHPEAKAAMANANSLLVL 540
           CMYQGNK RA +AGIV+ LLK+LSDS+ ++VD+ALYIMSVLC HPEAKAAM NANSLLVL
Sbjct: 553 CMYQGNKGRAFRAGIVKLLLKMLSDSNGTLVDDALYIMSVLCSHPEAKAAMGNANSLLVL 612

Query: 541 TEVLKTGASRSKENAAAVMFVLCKGDLEKLSWLTKLGATIPLMILAENGTGRARRKATSL 600
           T VLK G+SRS+ENA AV+  LCKGD EKL WLT+LGA +PLM L+E+GT RARRKA SL
Sbjct: 613 TNVLKKGSSRSRENAVAVLLALCKGDWEKLEWLTRLGAAVPLMKLSEDGTERARRKAASL 672

Query: 601 LEELRRS 604
           L++LR+S
Sbjct: 673 LDQLRKS 678

BLAST of Moc04g27320 vs. ExPASy TrEMBL
Match: A0A6J1JSX4 (RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111489474 PE=4 SV=1)

HSP 1 Score: 830.9 bits (2145), Expect = 3.5e-237
Identity = 446/607 (73.48%), Postives = 513/607 (84.51%), Query Frame = 0

Query: 1   MQKDCTDLIRRIALLIHLAEEIMNFRRG-GDNLAELNESGSSSSCS-LDCLSEVVGAIQA 60
           M+KDCTDLIRRIALLIHLAEEI NF  G  D    L +S SSSS S LDCLSEV+GAIQA
Sbjct: 1   MKKDCTDLIRRIALLIHLAEEITNFCGGFRDKFEALTDSISSSSSSCLDCLSEVIGAIQA 60

Query: 61  AKRLLYAALTFS--GNDDASAASTDGATKKLVYQFQYVTNRLQNALANLPYDHFCISDEV 120
           AKRLLY+A+TFS   +D+ S  ST+GATKKLV QF YVT+RL+ AL+NLPYDHFC++DEV
Sbjct: 61  AKRLLYSAMTFSVYDDDEVSLTSTEGATKKLVLQFHYVTSRLETALSNLPYDHFCVADEV 120

Query: 121 QEQVDLVRAQLRRAEKKYESMSELANKKLHSQGSVKWKVNNDVTSMSSVDYEVPESQHRP 180
           QEQVDLVRAQL RA K YESMS+  +KKL + GSVK  + +DV +MSSVD   P+SQH P
Sbjct: 121 QEQVDLVRAQLMRASKNYESMSDPTHKKLEATGSVKAMIFHDVKTMSSVDNGDPDSQHCP 180

Query: 181 QNRDYQVGFDPEEPKASDLCMDECSSVVQSDMEDVQATKSQEEVEKSDEIMIPENFLCPI 240
            NRD  + FD +E K S+ C +ECSS V S+MEDV + KSQ+EV K D I IPENFLC I
Sbjct: 181 PNRDDLIRFDSDEIKNSNPCFNECSS-VHSEMEDVLSIKSQDEVGKLDVIEIPENFLCSI 240

Query: 241 SFELMVDPVIISTGQTYERSNIQRWIDKGNKTCPKTQEQLQALILTPNFLMKQLISEWCQ 300
           S ELM+DPVI+STGQTYER NIQ+WID+GN TCPKTQEQLQ+LILTPNF M+ LISEWC 
Sbjct: 241 SLELMIDPVIVSTGQTYERYNIQKWIDRGNTTCPKTQEQLQSLILTPNFAMRNLISEWCG 300

Query: 301 EHNVKLEEGLTTRKIKKYRSFEDGSRKRVPVKTLVRHLSFGSAHEKKAAVTEIRKLTKSS 360
           EHNV LE+GLT RK+KKYRSFEDG R+ +P+KTLVRHLSFGS  E+KAAVTEIR+L+KSS
Sbjct: 301 EHNVNLEKGLTNRKLKKYRSFEDGCRRTLPIKTLVRHLSFGSVQEQKAAVTEIRQLSKSS 360

Query: 361 SEHRVEIAEAGAIPQLVNLLTSEDVLTQENAISCILNLSLHEQNKRLVMLSGAVSYISQV 420
           S+HRVEIA+AGAIPQLV LLTSEDV TQENAISCILNLSLHE NKRL+ML GA SYISQV
Sbjct: 361 SDHRVEIAKAGAIPQLVILLTSEDVATQENAISCILNLSLHEPNKRLIMLHGAFSYISQV 420

Query: 421 LKFGSLEARECAAATLYSLSLADENKAVIGASGVIPGLLEILEIGSARGKKDAARALLNL 480
           LKFGS+E RECAA T+YSLSLADENKA+IGASGVIP L+EIL+IGS RG+KDAA ALLNL
Sbjct: 421 LKFGSMEGRECAAGTIYSLSLADENKAMIGASGVIPDLIEILQIGSPRGQKDAAGALLNL 480

Query: 481 CMYQGNKSRAVKAGIVRPLLKLLSDSDASMVDEALYIMSVLCVHPEAKAAMANANSLLVL 540
           CMYQGNK RA +AGIV+ LLK+LSDS+ ++VD+ALYIMSVLC HPEAKAAM NANSLLVL
Sbjct: 481 CMYQGNKGRAFRAGIVKLLLKMLSDSNGTLVDDALYIMSVLCSHPEAKAAMGNANSLLVL 540

Query: 541 TEVLKTGASRSKENAAAVMFVLCKGDLEKLSWLTKLGATIPLMILAENGTGRARRKATSL 600
           T VLK G+SRS+ENA AV+  LCKGD EKL WLT+LGA +PLM L+E+GT RARRKA SL
Sbjct: 541 TNVLKKGSSRSRENAVAVLLALCKGDWEKLEWLTRLGAAVPLMKLSEDGTERARRKAASL 600

Query: 601 LEELRRS 604
           L++LR+S
Sbjct: 601 LDQLRKS 606

BLAST of Moc04g27320 vs. TAIR 10
Match: AT1G23030.1 (ARM repeat superfamily protein )

HSP 1 Score: 500.7 bits (1288), Expect = 1.6e-141
Identity = 302/613 (49.27%), Postives = 407/613 (66.39%), Query Frame = 0

Query: 2   QKDCTDLIRRIALLIHLAEEIMNFRRGGDNLAELNESGSSSSCSLDCLSEVVGAIQAAKR 61
           +KDC DL RR+ LL HL EEI +        +   +S +SSS   D  S++V  +QAAKR
Sbjct: 29  KKDCADLTRRVCLLTHLLEEIRD--------STPIDSAASSSSENDWWSDLVVGLQAAKR 88

Query: 62  LLYAALTFSGNDDASAASTDGATKKLVYQFQYVTNRLQNALANLPYDHFCISDEVQEQVD 121
           LL  A  F   D     S+DGA K++ +QFQ VT +L+ AL+NLPYD + ISDEV EQV+
Sbjct: 89  LLSTA-RFQARD-----SSDGAAKRISFQFQCVTWKLEKALSNLPYDLYDISDEVGEQVE 148

Query: 122 LVRAQLRRAEKKYESMSELANKKLHSQGSVKWKVNNDVTSMSSVDYE-VPESQHRPQNRD 181
           L R+QLRRA ++Y S++        S+   +   +N +   +    E V E+ H      
Sbjct: 149 LARSQLRRAMQRYGSLNSNKFSSALSEPMERDGFSNVIKIKAEEKLESVSETLH------ 208

Query: 182 YQVGFDPEEPKASDLCMDECSSV---------VQSDMEDVQATKSQEEVEKSDEIMIPEN 241
               F  EE K S   +   SS+           +D  D    K+ +E +KSD++ IP +
Sbjct: 209 ----FGEEEEKQSSPPLRRSSSISLAYYLSKDADTDRLDKMVNKNTDESKKSDKLTIPVD 268

Query: 242 FLCPISFELMVDPVIISTGQTYERSNIQRWIDKGNKTCPKTQEQLQALILTPNFLMKQLI 301
           FLCP+S ELM DPVI++TGQTYER+ IQRWID GN TCPKTQ++L+   LTPN++++ LI
Sbjct: 269 FLCPVSLELMKDPVIVATGQTYERAYIQRWIDCGNLTCPKTQQKLENFTLTPNYVLRSLI 328

Query: 302 SEWCQEHNVKLEEGLTTRKIKKYRSFEDGSRKRVPVKTLVRHLSFGSAHEKKAAVTEIRK 361
           S WC EHN++   G    + K        S     ++ LV+ LS  S  +++ AV+EIR 
Sbjct: 329 SRWCAEHNIEQPAGYINGRTK-------NSGDMSVIRALVQRLSSRSTEDRRNAVSEIRS 388

Query: 362 LTKSSSEHRVEIAEAGAIPQLVNLLTSEDVLTQENAISCILNLSLHEQNKRLVMLSGAVS 421
           L+K S+++R+ IAEAGAIP LVNLLTSEDV TQENAI+C+LNLS++E NK L+M +GAV+
Sbjct: 389 LSKRSTDNRILIAEAGAIPVLVNLLTSEDVATQENAITCVLNLSIYENNKELIMFAGAVT 448

Query: 422 YISQVLKFGSLEARECAAATLYSLSLADENKAVIGASGVIPGLLEILEIGSARGKKDAAR 481
            I QVL+ G++EARE AAATL+SLSLADENK +IG SG IP L+++LE G+ RGKKDAA 
Sbjct: 449 SIVQVLRAGTMEARENAAATLFSLSLADENKIIIGGSGAIPALVDLLENGTPRGKKDAAT 508

Query: 482 ALLNLCMYQGNKSRAVKAGIVRPLLKLLSDSDA-SMVDEALYIMSVLCVHPEAKAAMANA 541
           AL NLC+Y GNK RAV+AGIV  L+K+LSDS    MVDEAL I+SVL  + +AK+A+  A
Sbjct: 509 ALFNLCIYHGNKGRAVRAGIVTALVKMLSDSTRHRMVDEALTILSVLANNQDAKSAIVKA 568

Query: 542 NSLLVLTEVLKTGASRSKENAAAVMFVLCKGDLEKLSWLTKLGATIPLMILAENGTGRAR 601
           N+L  L  +L+T  +R++ENAAA++  LCK D EKL  + +LGA +PLM L++NGT R +
Sbjct: 569 NTLPALIGILQTDQTRNRENAAAILLSLCKRDTEKLITIGRLGAVVPLMDLSKNGTERGK 610

Query: 602 RKATSLLEELRRS 604
           RKA SLLE LR++
Sbjct: 629 RKAISLLELLRKA 610

BLAST of Moc04g27320 vs. TAIR 10
Match: AT1G71020.1 (ARM repeat superfamily protein )

HSP 1 Score: 493.8 bits (1270), Expect = 1.9e-139
Identity = 301/612 (49.18%), Postives = 413/612 (67.48%), Query Frame = 0

Query: 2   QKDCTDLIRRIALLIHLAEEIMNFRRGGDNLAELNESGSSSSCSLDCLSEVVGAIQAAKR 61
           +KDC+DL RR+ LL HL EEI    R     +E + S S +S   D  S++V  +QAAKR
Sbjct: 29  KKDCSDLARRVGLLTHLIEEI----RDSSPPSESDASSSLNSHECDWWSDLVVGLQAAKR 88

Query: 62  LLYAALTFSGNDDASAASTDGATKKLVYQFQYVTNRLQNALANLPYDHFCISDEVQEQVD 121
           LL +A +F   +     S+DGA K++ +QFQ VT +L+ AL +L YD + ISDEV+EQV+
Sbjct: 89  LLSSATSFQARE-----SSDGAAKRISFQFQCVTWKLEKALGDLTYDRYDISDEVREQVE 148

Query: 122 LVRAQLRRAEKKYESMSELANKKLHSQGSVKWKVNNDVTSMSSVDYE---VPESQHRPQN 181
           L R QLRRA ++Y S+    N K  S G +   +  D +S   V  +   +PE+ H    
Sbjct: 149 LARLQLRRAMQRYGSL----NSKKFSSG-LSEPMEKDASSNRKVIEKLESIPETVH--SL 208

Query: 182 RDYQVGFDPEEPKASDLCMDECSSVVQSDMEDVQ----ATKSQEEVEKSDEIMIPENFLC 241
            D +    P   K+S + +   +  +  D +D +     T++ ++ +KSD + IPE+FLC
Sbjct: 209 SDEKKFESPPPWKSSSVSL---AFFLSKDGDDERLEKAVTENSDDSQKSDNLTIPEDFLC 268

Query: 242 PISFELMVDPVIISTGQTYERSNIQRWIDKGNKTCPKTQEQLQALILTPNFLMKQLISEW 301
           PIS ELM DP I+STGQTYERS IQRWID GN +CPKTQ++L+   LTPN++++ LIS+W
Sbjct: 269 PISLELMKDPAIVSTGQTYERSFIQRWIDCGNLSCPKTQQKLENFTLTPNYVLRSLISQW 328

Query: 302 CQEHNVKLEEGLTTRKIKKY-RSFEDGSRKRVPVKTLVRHLSFGSAHEKKAAVTEIRKLT 361
           C +HN++   G    + K    SF D S     ++ LV  LS  S  +++ AV+EIR L+
Sbjct: 329 CTKHNIEQPGGYMNGRTKNSDGSFRDLSGDMSAIRALVCKLSSQSIEDRRTAVSEIRSLS 388

Query: 362 KSSSEHRVEIAEAGAIPQLVNLLTSE-DVLTQENAISCILNLSLHEQNKRLVMLSGAVSY 421
           K S+++R+ IAEAGAIP LV LLTS+ D  TQENA++CILNLS++E NK L+ML+GAV+ 
Sbjct: 389 KRSTDNRILIAEAGAIPVLVKLLTSDGDTETQENAVTCILNLSIYEHNKELIMLAGAVTS 448

Query: 422 ISQVLKFGSLEARECAAATLYSLSLADENKAVIGASGVIPGLLEILEIGSARGKKDAARA 481
           I  VL+ GS+EARE AAATL+SLSLADENK +IGASG I  L+++L+ GS RGKKDAA A
Sbjct: 449 IVLVLRAGSMEARENAAATLFSLSLADENKIIIGASGAIMALVDLLQYGSVRGKKDAATA 508

Query: 482 LLNLCMYQGNKSRAVKAGIVRPLLKLLSDSDAS-MVDEALYIMSVLCVHPEAKAAMANAN 541
           L NLC+YQGNK RAV+AGIV+PL+K+L+DS +  M DEAL I+SVL  +  AK A+  AN
Sbjct: 509 LFNLCIYQGNKGRAVRAGIVKPLVKMLTDSSSERMADEALTILSVLASNQVAKTAILRAN 568

Query: 542 SLLVLTEVLKTGASRSKENAAAVMFVLCKGDLEKLSWLTKLGATIPLMILAENGTGRARR 601
           ++  L + L+    R++ENAAA++  LCK D EKL  + +LGA +PLM L+ +GT RA+R
Sbjct: 569 AIPPLIDCLQKDQPRNRENAAAILLCLCKRDTEKLISIGRLGAVVPLMELSRDGTERAKR 621

Query: 602 KATSLLEELRRS 604
           KA SLLE LR+S
Sbjct: 629 KANSLLELLRKS 621

BLAST of Moc04g27320 vs. TAIR 10
Match: AT1G71020.2 (ARM repeat superfamily protein )

HSP 1 Score: 404.4 bits (1038), Expect = 1.6e-112
Identity = 221/393 (56.23%), Postives = 294/393 (74.81%), Query Frame = 0

Query: 214 TKSQEEVEKSDEIMIPENFLCPISFELMVDPVIISTGQTYERSNIQRWIDKGNKTCPKTQ 273
           T++ ++ +KSD + IPE+FLCPIS ELM DP I+STGQTYERS IQRWID GN +CPKTQ
Sbjct: 81  TENSDDSQKSDNLTIPEDFLCPISLELMKDPAIVSTGQTYERSFIQRWIDCGNLSCPKTQ 140

Query: 274 EQLQALILTPNFLMKQLISEWCQEHNVKLEEGLTTRKIKKY-RSFEDGSRKRVPVKTLVR 333
           ++L+   LTPN++++ LIS+WC +HN++   G    + K    SF D S     ++ LV 
Sbjct: 141 QKLENFTLTPNYVLRSLISQWCTKHNIEQPGGYMNGRTKNSDGSFRDLSGDMSAIRALVC 200

Query: 334 HLSFGSAHEKKAAVTEIRKLTKSSSEHRVEIAEAGAIPQLVNLLTSE-DVLTQENAISCI 393
            LS  S  +++ AV+EIR L+K S+++R+ IAEAGAIP LV LLTS+ D  TQENA++CI
Sbjct: 201 KLSSQSIEDRRTAVSEIRSLSKRSTDNRILIAEAGAIPVLVKLLTSDGDTETQENAVTCI 260

Query: 394 LNLSLHEQNKRLVMLSGAVSYISQVLKFGSLEARECAAATLYSLSLADENKAVIGASGVI 453
           LNLS++E NK L+ML+GAV+ I  VL+ GS+EARE AAATL+SLSLADENK +IGASG I
Sbjct: 261 LNLSIYEHNKELIMLAGAVTSIVLVLRAGSMEARENAAATLFSLSLADENKIIIGASGAI 320

Query: 454 PGLLEILEIGSARGKKDAARALLNLCMYQGNKSRAVKAGIVRPLLKLLSDSDAS-MVDEA 513
             L+++L+ GS RGKKDAA AL NLC+YQGNK RAV+AGIV+PL+K+L+DS +  M DEA
Sbjct: 321 MALVDLLQYGSVRGKKDAATALFNLCIYQGNKGRAVRAGIVKPLVKMLTDSSSERMADEA 380

Query: 514 LYIMSVLCVHPEAKAAMANANSLLVLTEVLKTGASRSKENAAAVMFVLCKGDLEKLSWLT 573
           L I+SVL  +  AK A+  AN++  L + L+    R++ENAAA++  LCK D EKL  + 
Sbjct: 381 LTILSVLASNQVAKTAILRANAIPPLIDCLQKDQPRNRENAAAILLCLCKRDTEKLISIG 440

Query: 574 KLGATIPLMILAENGTGRARRKATSLLEELRRS 604
           +LGA +PLM L+ +GT RA+RKA SLLE LR+S
Sbjct: 441 RLGAVVPLMELSRDGTERAKRKANSLLELLRKS 473

BLAST of Moc04g27320 vs. TAIR 10
Match: AT3G54850.1 (plant U-box 14 )

HSP 1 Score: 388.3 bits (996), Expect = 1.2e-107
Identity = 244/613 (39.80%), Postives = 378/613 (61.66%), Query Frame = 0

Query: 7   DLIRRIALLIHLAEEI--MNFRRGGDNLAELNESGSSSSCSLDCLSEVVGAIQAAKRLLY 66
           DL+RRI LL    EE+  +N     D +        +   +LD   E+  ++    +L  
Sbjct: 39  DLVRRITLLSPFFEELIDVNVELKKDQITGF----EAMRIALDSSLELFRSVNGGSKL-- 98

Query: 67  AALTFSGNDDASAASTDGATKKLVYQFQYVTNRLQNALANLPYDHFCISDEVQEQVDLVR 126
               F   D  S          LV +F+ +T  ++ AL+ +PY+   +S+EV+EQV L+ 
Sbjct: 99  ----FQLFDRDS----------LVEKFRDMTVEIEAALSQIPYEKIEVSEEVREQVQLLH 158

Query: 127 AQLRRAEKKYESMS-------ELANKKLHSQGSVKWKVNNDVTSMSSVDYEVPESQHRPQ 186
            Q +RA++++E           +A   +     +  +++ ++  ++++D E+ +  H   
Sbjct: 159 FQFKRAKERWEESDLQLSHDLAMAENVMDPDPIILKRLSQEL-QLTTID-ELKKESH--A 218

Query: 187 NRDYQVGFDPEEPKASDLCMDECSSVVQSDMEDVQATKSQEEVEKSDEI-------MIPE 246
             +Y + +D +     D C +  SS++++ ++ V    S  +      I       +IPE
Sbjct: 219 IHEYFLSYDGD----PDDCFERMSSLLKNLVDFVTMESSDPDPSTGSRIVSRHRSPVIPE 278

Query: 247 NFLCPISFELMVDPVIISTGQTYERSNIQRWIDKGNKTCPKTQEQLQALILTPNFLMKQL 306
            F CPIS ELM DPVI+STGQTYERS+IQ+W+D G+KTCPK+QE L    LTPN+++K L
Sbjct: 279 YFRCPISLELMKDPVIVSTGQTYERSSIQKWLDAGHKTCPKSQETLLHAGLTPNYVLKSL 338

Query: 307 ISEWCQEHNVKLEEGLTTRKIKKYRSFEDGSRKRVPVKTLVRHLSFGSAHEKKAAVTEIR 366
           I+ WC+ + ++L +   + +  K          R  V +L+  L+ G+  +++AA  E+R
Sbjct: 339 IALWCESNGIELPQNQGSCRTTKIGGSSSSDCDRTFVLSLLEKLANGTTEQQRAAAGELR 398

Query: 367 KLTKSSSEHRVEIAEAGAIPQLVNLLTSEDVLTQENAISCILNLSLHEQNKRLVMLSGAV 426
            L K + ++RV IAEAGAIP LV LL+S D  TQE++++ +LNLS++E NK  ++ +GA+
Sbjct: 399 LLAKRNVDNRVCIAEAGAIPLLVELLSSPDPRTQEHSVTALLNLSINEGNKGAIVDAGAI 458

Query: 427 SYISQVLKFGSLEARECAAATLYSLSLADENKAVIGASGVIPGLLEILEIGSARGKKDAA 486
           + I +VLK GS+EARE AAATL+SLS+ DENK  IGA+G I  L+ +LE G+ RGKKDAA
Sbjct: 459 TDIVEVLKNGSMEARENAAATLFSLSVIDENKVAIGAAGAIQALISLLEEGTRRGKKDAA 518

Query: 487 RALLNLCMYQGNKSRAVKAGIVRPLLKLLSDSDASMVDEALYIMSVLCVHPEAKAAMANA 546
            A+ NLC+YQGNKSRAVK GIV PL +LL D+   MVDEAL I+++L  + E K A+A A
Sbjct: 519 TAIFNLCIYQGNKSRAVKGGIVDPLTRLLKDAGGGMVDEALAILAILSTNQEGKTAIAEA 578

Query: 547 NSLLVLTEVLKTGASRSKENAAAVMFVLCKGDLEKLSWLTKLGATIPLMILAENGTGRAR 604
            S+ VL E+++TG+ R++ENAAA+++ LC G++E+L+   ++GA + L  L ENGT RA+
Sbjct: 579 ESIPVLVEIIRTGSPRNRENAAAILWYLCIGNIERLNVAREVGADVALKELTENGTDRAK 623

BLAST of Moc04g27320 vs. TAIR 10
Match: AT3G46510.1 (plant U-box 13 )

HSP 1 Score: 367.1 bits (941), Expect = 2.7e-101
Identity = 247/635 (38.90%), Postives = 375/635 (59.06%), Query Frame = 0

Query: 1   MQKDCTDLIRRIALLIHLAEEIMNFRRGGDNLAELNESGSSSSCSLDCLSEVVGAIQAAK 60
           ++K C +L RR+ LL+ + EEI           E NE  S    +L  L  +  A+ +AK
Sbjct: 29  VKKLCYNLARRLKLLVPMFEEI----------RESNEPISED--TLKTLMNLKEAMCSAK 88

Query: 61  RLLYAALTFSGNDDASAASTDGATKKLVYQFQYVTNRLQNALANLPYDHFCISDEVQEQV 120
              Y      G+        +  T KL+     V+ +L+ +L+ +PY+   ISDEV+EQV
Sbjct: 89  D--YLKFCSQGSKIYLVMEREQVTSKLM----EVSVKLEQSLSQIPYEELDISDEVREQV 148

Query: 121 DLVRAQLRRA--------EKKYESMSELANKKLHSQGSVKWKVNNDVTSMSSVDYEVPES 180
           +LV +Q RRA        ++ YE +  L NK            ++DV +   V   V + 
Sbjct: 149 ELVLSQFRRAKGRVDVSDDELYEDLQSLCNK------------SSDVDAYQPVLERVAKK 208

Query: 181 QHRPQNRDY-QVGFDPEEPKASD-----LCMDECSSVVQSDMEDVQATKSQEEVEK---- 240
            H  +  D  Q      E  AS        ++E + V++   + VQ      E +K    
Sbjct: 209 LHLMEIPDLAQESVALHEMVASSGGDVGENIEEMAMVLKMIKDFVQTEDDNGEEQKVGVN 268

Query: 241 -----------SDEI-MIPENFLCPISFELMVDPVIISTGQTYERSNIQRWIDKGNKTCP 300
                      S +I +IP++F CPIS E+M DPVI+S+GQTYER+ I++WI+ G+ TCP
Sbjct: 269 SRSNGQTSTAASQKIPVIPDDFRCPISLEMMRDPVIVSSGQTYERTCIEKWIEGGHSTCP 328

Query: 301 KTQEQLQALILTPNFLMKQLISEWCQEHNV---KLEEGLTTRKIKKYRSFEDGSRKRVPV 360
           KTQ+ L +  LTPN++++ LI++WC+ +++   K    L  RK+  + S  + ++    +
Sbjct: 329 KTQQALTSTTLTPNYVLRSLIAQWCEANDIEPPKPPSSLRPRKVSSFSSPAEANK----I 388

Query: 361 KTLVRHLSFGSAHEKKAAVTEIRKLTKSSSEHRVEIAEAGAIPQLVNLLTSEDVLTQENA 420
           + L+  L++G+  ++++A  EIR L K ++++RV IAEAGAIP LV LL++ D   QE++
Sbjct: 389 EDLMWRLAYGNPEDQRSAAGEIRLLAKRNADNRVAIAEAGAIPLLVGLLSTPDSRIQEHS 448

Query: 421 ISCILNLSLHEQNKRLVMLSGAVSYISQVLKFGSLEARECAAATLYSLSLADENKAVIGA 480
           ++ +LNLS+ E NK  ++ +GA+  I QVLK GS+EARE AAATL+SLS+ DENK  IGA
Sbjct: 449 VTALLNLSICENNKGAIVSAGAIPGIVQVLKKGSMEARENAAATLFSLSVIDENKVTIGA 508

Query: 481 SGVIPGLLEILEIGSARGKKDAARALLNLCMYQGNKSRAVKAGIVRPLLKLLSDSDASMV 540
            G IP L+ +L  G+ RGKKDAA AL NLC+YQGNK +A++AG++  L +LL++  + MV
Sbjct: 509 LGAIPPLVVLLNEGTQRGKKDAATALFNLCIYQGNKGKAIRAGVIPTLTRLLTEPGSGMV 568

Query: 541 DEALYIMSVLCVHPEAKAAMANANSLLVLTEVLKTGASRSKENAAAVMFVLCKGDLEKLS 600
           DEAL I+++L  HPE KA + +++++  L E ++TG+ R++ENAAAV+  LC GD + L 
Sbjct: 569 DEALAILAILSSHPEGKAIIGSSDAVPSLVEFIRTGSPRNRENAAAVLVHLCSGDPQHLV 628

Query: 601 WLTKLGATIPLMILAENGTGRARRKATSLLEELRR 603
              KLG   PL+ LA NGT R +RKA  LLE + R
Sbjct: 629 EAQKLGLMGPLIDLAGNGTDRGKRKAAQLLERISR 629

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022152334.10.0e+00100.00U-box domain-containing protein 11-like isoform X1 [Momordica charantia][more]
XP_022152335.10.0e+00100.00U-box domain-containing protein 11-like isoform X2 [Momordica charantia][more]
XP_038875665.13.0e-25177.43U-box domain-containing protein 11-like isoform X3 [Benincasa hispida][more]
XP_038875664.13.0e-25177.43U-box domain-containing protein 11-like isoform X2 [Benincasa hispida][more]
XP_038875663.13.0e-25177.43U-box domain-containing protein 11-like isoform X1 [Benincasa hispida][more]
Match NameE-valueIdentityDescription
Q8GUG92.2e-14049.27U-box domain-containing protein 11 OS=Arabidopsis thaliana OX=3702 GN=PUB11 PE=2... [more]
Q9C9A62.7e-13849.18U-box domain-containing protein 10 OS=Arabidopsis thaliana OX=3702 GN=PUB10 PE=2... [more]
Q8VZ401.6e-10639.80U-box domain-containing protein 14 OS=Arabidopsis thaliana OX=3702 GN=PUB14 PE=1... [more]
Q9SNC63.9e-10038.90U-box domain-containing protein 13 OS=Arabidopsis thaliana OX=3702 GN=PUB13 PE=1... [more]
Q5VRH95.0e-10039.84U-box domain-containing protein 12 OS=Oryza sativa subsp. japonica OX=39947 GN=P... [more]
Match NameE-valueIdentityDescription
A0A6J1DFQ40.0e+00100.00RING-type E3 ubiquitin transferase OS=Momordica charantia OX=3673 GN=LOC11102007... [more]
A0A6J1DDN20.0e+00100.00RING-type E3 ubiquitin transferase OS=Momordica charantia OX=3673 GN=LOC11102007... [more]
A0A1S3CSU47.3e-24374.84RING-type E3 ubiquitin transferase OS=Cucumis melo OX=3656 GN=LOC103504572 PE=4 ... [more]
A0A6J1K2B73.5e-23773.48RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111489474 P... [more]
A0A6J1JSX43.5e-23773.48RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111489474 P... [more]
Match NameE-valueIdentityDescription
AT1G23030.11.6e-14149.27ARM repeat superfamily protein [more]
AT1G71020.11.9e-13949.18ARM repeat superfamily protein [more]
AT1G71020.21.6e-11256.23ARM repeat superfamily protein [more]
AT3G54850.11.2e-10739.80plant U-box 14 [more]
AT3G46510.12.7e-10138.90plant U-box 13 [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (OHB3-1) v2
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 113..140
NoneNo IPR availablePANTHERPTHR23315U BOX DOMAIN-CONTAININGcoord: 2..602
NoneNo IPR availablePANTHERPTHR23315:SF52U-BOX DOMAIN-CONTAINING PROTEIN 11coord: 2..602
NoneNo IPR availableCDDcd16664RING-Ubox_PUBcoord: 232..274
e-value: 3.36927E-25
score: 96.1486
NoneNo IPR availableSUPERFAMILY57850RING/U-boxcoord: 221..301
IPR003613U box domainSMARTSM00504Ubox_2coord: 231..294
e-value: 7.6E-29
score: 111.8
IPR003613U box domainPFAMPF04564U-boxcoord: 228..299
e-value: 7.3E-22
score: 77.3
IPR003613U box domainPROSITEPS51698U_BOXcoord: 227..301
score: 38.975922
IPR000225ArmadilloSMARTSM00185arm_5coord: 397..437
e-value: 25.0
score: 10.1
coord: 479..519
e-value: 6.2
score: 14.8
coord: 438..478
e-value: 2.0
score: 17.4
coord: 520..560
e-value: 80.0
score: 6.2
coord: 356..396
e-value: 3.0E-4
score: 30.1
IPR000225ArmadilloPFAMPF00514Armcoord: 481..518
e-value: 3.1E-4
score: 20.7
coord: 439..477
e-value: 1.4E-4
score: 21.8
coord: 356..395
e-value: 1.9E-9
score: 37.2
IPR000225ArmadilloPROSITEPS50176ARM_REPEATcoord: 367..409
score: 12.8274
IPR011989Armadillo-like helicalGENE3D1.25.10.10coord: 424..602
e-value: 1.5E-25
score: 91.9
IPR011989Armadillo-like helicalGENE3D1.25.10.10coord: 321..423
e-value: 1.7E-18
score: 68.9
IPR013083Zinc finger, RING/FYVE/PHD-typeGENE3D3.30.40.10Zinc/RING finger domain, C3HC4 (zinc finger)coord: 207..311
e-value: 2.5E-29
score: 103.2
IPR016024Armadillo-type foldSUPERFAMILY48371ARM repeatcoord: 327..600

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Moc04g27320.1Moc04g27320.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0016567 protein ubiquitination
molecular_function GO:0005515 protein binding
molecular_function GO:0004842 ubiquitin-protein transferase activity