Moc04g27100 (gene) Bitter gourd (OHB3-1) v2

Overview


Name	Moc04g27100
Type	gene
Organism	Momordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
Description	Ribonuclease H
Location	chr4: 19961569 .. 19962357 (-)
RNA-Seq Expression	Moc04g27100
Synteny	Moc04g27100

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGCCTGTTCTGCCGTCAGCACATCCAAAGCCATCCAAGGCCAATCGTGGCCGAGGTGGAGCTTCGAAGAGAACCACCCGGGGACCAGCCCCAGCTCCAACAAGGGAAAACTTCGATGCACTTCAGAAAGAAATGGAGGCAATGAGCACTCAGATGCGCACCATGGAAGAAATGTACAACGAGATGGTGCAAGCCGCAGGTGCTAGGTCTCAATCTGAAGACCGAGTGGCACGCGGCGAGGTGCACGAGCGCAGAGGTCTTCACCTCGGACCAGTCGAGGAAGAATGCCCTGGAGGAGATGAAGATGAAGAGTACACTCATCAGAGGGGTGATCTCCGCGAGCATCTCAACAGAAAGAGAAGCTCGTCCCTCAGAAAGGGACGATCTCCGTCCTGCTCGCACAAGAGCTCCAACCAGCAGGCTGAGTCCTCCTACAACCCAATAACTCCCGAGGGAGTGATCACAAGGGAGGAGTTCAATTAGCTAAAGAGCAAGTTCGATGCTTAGGTTGAAGCCTTGAAGGCTAGGTGCGAGAAAAAAGAAAATTCGTTCGATAATGGCGACCTAGGAGAATCACCATTCACTTCGGACATCCTGGAGGCTCCAATCCCTCCGAAGTTCAAAACTCCCACCATAAAGCTTTATGATGGGTCTAAGGACCCAAAAGACTATGTTGAGGTCTTCGAAGGTCTCATGGATTTTCAGGCGGCAACAGACGCAATCAAGTGCCGCACCTTCCAGATCGCACTCACCGGCAGCGCGATTATGCTATCGAAGACTGCCGGCTAG

mRNA sequence

ATGCCTGTTCTGCCGTCAGCACATCCAAAGCCATCCAAGGCCAATCGTGGCCGAGGTGGAGCTTCGAAGAGAACCACCCGGGGACCAGCCCCAGCTCCAACAAGGGAAAACTTCGATGCACTTCAGAAAGAAATGGAGGCAATGAGCACTCAGATGCGCACCATGGAAGAAATGTACAACGAGATGGTGCAAGCCGCAGGTGCTAGGTCTCAATCTGAAGACCGAGTGGCACGCGGCGAGGTGCACGAGCGCAGAGGTCTTCACCTCGGACCAGTCGAGGAAGAATGCCCTGGAGGAGATGAAGATGAAGAGTACACTCATCAGAGGGGTGATCTCCGCGAGCATCTCAACAGAAAGAGAAGCTCGTCCCTCAGAAAGGGACGATCTCCGTCCTGCTCGCACAAGAGCTCCAACCAGCAGGCTGAGTGCGAGAAAAAAGAAAATTCGTTCGATAATGGCGACCTAGGAGAATCACCATTCACTTCGGACATCCTGGAGGCTCCAATCCCTCCGAAGTTCAAAACTCCCACCATAAAGCTTTATGATGGGTCTAAGGACCCAAAAGACTATGTTGAGGTCTTCGAAGGTCTCATGGATTTTCAGGCGGCAACAGACGCAATCAAGTGCCGCACCTTCCAGATCGCACTCACCGGCAGCGCGATTATGCTATCGAAGACTGCCGGCTAG

Coding sequence (CDS)

ATGCCTGTTCTGCCGTCAGCACATCCAAAGCCATCCAAGGCCAATCGTGGCCGAGGTGGAGCTTCGAAGAGAACCACCCGGGGACCAGCCCCAGCTCCAACAAGGGAAAACTTCGATGCACTTCAGAAAGAAATGGAGGCAATGAGCACTCAGATGCGCACCATGGAAGAAATGTACAACGAGATGGTGCAAGCCGCAGGTGCTAGGTCTCAATCTGAAGACCGAGTGGCACGCGGCGAGGTGCACGAGCGCAGAGGTCTTCACCTCGGACCAGTCGAGGAAGAATGCCCTGGAGGAGATGAAGATGAAGAGTACACTCATCAGAGGGGTGATCTCCGCGAGCATCTCAACAGAAAGAGAAGCTCGTCCCTCAGAAAGGGACGATCTCCGTCCTGCTCGCACAAGAGCTCCAACCAGCAGGCTGAGTGCGAGAAAAAAGAAAATTCGTTCGATAATGGCGACCTAGGAGAATCACCATTCACTTCGGACATCCTGGAGGCTCCAATCCCTCCGAAGTTCAAAACTCCCACCATAAAGCTTTATGATGGGTCTAAGGACCCAAAAGACTATGTTGAGGTCTTCGAAGGTCTCATGGATTTTCAGGCGGCAACAGACGCAATCAAGTGCCGCACCTTCCAGATCGCACTCACCGGCAGCGCGATTATGCTATCGAAGACTGCCGGCTAG

Protein sequence

MPVLPSAHPKPSKANRGRGGASKRTTRGPAPAPTRENFDALQKEMEAMSTQMRTMEEMYNEMVQAAGARSQSEDRVARGEVHERRGLHLGPVEEECPGGDEDEEYTHQRGDLREHLNRKRSSSLRKGRSPSCSHKSSNQQAECEKKENSFDNGDLGESPFTSDILEAPIPPKFKTPTIKLYDGSKDPKDYVEVFEGLMDFQAATDAIKCRTFQIALTGSAIMLSKTAG

Homology

[Close]

BLAST of Moc04g27100 vs. NCBI nr
Match: XP_022159327.1 (uncharacterized protein LOC111025738 [Momordica charantia])

HSP 1 Score: 266.2 bits (679), Expect = 2.7e-67
Identity = 160/253 (63.24%), Postives = 170/253 (67.19%), Query Frame = 0


Query: 2   PVLPSAHPKPSKANRGRGGASKRTTRGPAPAPTRENFDALQKEMEAMSTQMRTMEEMYNE 61
           P L  AHPKP KANRGRGGAS+RTT G APAP+RENFDALQKEMEAM TQM TMEEMYNE
Sbjct: 53  PDLSPAHPKPFKANRGRGGASRRTTLGAAPAPSRENFDALQKEMEAMRTQMLTMEEMYNE 112

Query: 62  MVQAAGARSQSEDRVARGEVHERRGLHLGPVEEECPGGDEDEEYTHQRGDLREHLNRKRS 121
           MVQA GA S+SEDR AR E                            RGDLR+HL+RKRS
Sbjct: 113 MVQAVGAGSRSEDRAARDE----------------------------RGDLRDHLSRKRS 172

Query: 122 SSLRKGRSPSCSHKSSNQQAE----------------------------------CEKKE 181
           SSLRKGRSPSCSHK+SNQQAE                                  CE K 
Sbjct: 173 SSLRKGRSPSCSHKNSNQQAESSYNPVVPEGVITREEFDQLKSKFDAQVETLKARCEVKG 232

Query: 182 NSFDNGDLGESPFTSDILEAPIPPKFKTPTIKLYDGSKDPKDYVEVFEGLMDFQAATDAI 221
           ++FD+GDLGESPFTSDILEA IP KFKTPT+K YDGSKDPKDYVEVFEGLM FQAATDAI
Sbjct: 233 STFDDGDLGESPFTSDILEALIPSKFKTPTMKPYDGSKDPKDYVEVFEGLMGFQAATDAI 277

[Close]

BLAST of Moc04g27100 vs. NCBI nr
Match: XP_022151719.1 (uncharacterized protein LOC111019634 [Momordica charantia])

HSP 1 Score: 265.0 bits (676), Expect = 6.0e-67
Identity = 146/207 (70.53%), Postives = 161/207 (77.78%), Query Frame = 0


Query: 48  MSTQMRTMEEMYNEMVQAAGARSQSEDRVARGEVHERRGLHLGPVEEECPGGDEDEEYTH 107
           M TQM TME+MY+EMVQAAGARS+SE+RVAR ++HE+RG HLGPV++  P G EDEEYTH
Sbjct: 1   MRTQMCTMEKMYSEMVQAAGARSRSENRVARNDMHEQRGHHLGPVKKGHPEGGEDEEYTH 60

Query: 108 QRGDLREHLNRKRSSSLRKGRSPSCSHKSSNQQAE------------------------- 167
           QRGDLREHLNRKRSSSLRKG+SPSCSH++SNQQAE                         
Sbjct: 61  QRGDLREHLNRKRSSSLRKGQSPSCSHRNSNQQAESSYNPITPEGVITREEFDQLKSKFD 120

Query: 168 ---------CEKKENSFDNGDLGESPFTSDILEAPIPPKFKTPTIKLYDGSKDPKDYVEV 221
                    CEKKE+SFD+GDLGESPFTSDILEA IP KFKTPT+K YDGSKDPKDYVEV
Sbjct: 121 AQVEALKAKCEKKESSFDDGDLGESPFTSDILEALIPLKFKTPTMKPYDGSKDPKDYVEV 180

[Close]

BLAST of Moc04g27100 vs. NCBI nr
Match: XP_022156088.1 (uncharacterized protein LOC111023060 [Momordica charantia])

HSP 1 Score: 220.3 bits (560), Expect = 1.7e-53
Identity = 129/210 (61.43%), Postives = 144/210 (68.57%), Query Frame = 0


Query: 45  MEAMSTQMRTMEEMYNEMVQAAGARSQSEDRVARGEVHERRGLHLGPVEEECPGGDEDEE 104
           MEAM TQMRTMEEMYN+MVQ AGARS+S D+V   +VHE+  LH  PV+EE         
Sbjct: 1   MEAMRTQMRTMEEMYNKMVQIAGARSRSGDQVVHEDVHEQGDLHCDPVDEE--------- 60

Query: 105 YTHQRGDLREHLNRKRSSSLRKGRSPSCSHKSSNQQAE---------------------- 164
             H  GDLR+HLNRKR+SS R  R+ +  HK+SNQQAE                      
Sbjct: 61  --HLGGDLRDHLNRKRNSSHRGERTSTYWHKNSNQQAESSYNPIAPEGVITREEFNQLKS 120

Query: 165 ------------CEKKENSFDNGDLGESPFTSDILEAPIPPKFKTPTIKLYDGSKDPKDY 221
                       CEKKE++FD+GDLGESPFTSDILEA IPPKFKTPT+K YDGSKDPKDY
Sbjct: 121 KFDAQVEALKVRCEKKESAFDDGDLGESPFTSDILEASIPPKFKTPTMKSYDGSKDPKDY 180

[Close]

BLAST of Moc04g27100 vs. NCBI nr
Match: XP_022155128.1 (uncharacterized protein LOC111022267 [Momordica charantia])

HSP 1 Score: 189.5 bits (480), Expect = 3.2e-44
Identity = 104/154 (67.53%), Postives = 113/154 (73.38%), Query Frame = 0


Query: 101 EDEEYTHQRGDLREHLNRKRSSSLRKGRSPSCSHKSSNQQAE------------------ 160
           EDEEYTHQRGDLREHLNRKRSSSLRKG+SPS SH++SNQQAE                  
Sbjct: 33  EDEEYTHQRGDLREHLNRKRSSSLRKGQSPSSSHRNSNQQAESSYNPITPKRVITREEFD 92

Query: 161 ----------------CEKKENSFDNGDLGESPFTSDILEAPIPPKFKTPTIKLYDGSKD 220
                           CEKKE+SFD+GDLGE PFT DILEAPI PKFKTPT+K YDGSK+
Sbjct: 93  QLKSKFDAQVEALKANCEKKESSFDDGDLGELPFTLDILEAPIHPKFKTPTMKPYDGSKN 152

[Close]

BLAST of Moc04g27100 vs. NCBI nr
Match: XP_022158652.1 (uncharacterized protein LOC111025109 [Momordica charantia])

HSP 1 Score: 155.2 bits (391), Expect = 6.7e-34
Identity = 84/135 (62.22%), Postives = 96/135 (71.11%), Query Frame = 0


Query: 117 NRKRSSSLRKGRSPSCSHKSSNQQAE-------------------------------CEK 176
           + KR SSLRKG+SPS SH+SSNQQAE                               CE+
Sbjct: 3   HEKRGSSLRKGQSPSRSHRSSNQQAESSHNPAGIITREEFDQLRGELDAQVEALKAKCEQ 62

Query: 177 KENSFDNGDLGESPFTSDILEAPIPPKFKTPTIKLYDGSKDPKDYVEVFEGLMDFQAATD 221
           K++S ++GDLGE PFTSD+LEAPIPPKFK PT+K YDG+KDPKDYVEVFEGLMDFQAA+D
Sbjct: 63  KDDSLNDGDLGEPPFTSDVLEAPIPPKFKAPTVKPYDGTKDPKDYVEVFEGLMDFQAASD 122

[Close]

BLAST of Moc04g27100 vs. ExPASy TrEMBL
Match: A0A6J1DZJ1 (uncharacterized protein LOC111025738 OS=Momordica charantia OX=3673 GN=LOC111025738 PE=4 SV=1)

HSP 1 Score: 266.2 bits (679), Expect = 1.3e-67
Identity = 160/253 (63.24%), Postives = 170/253 (67.19%), Query Frame = 0


Query: 2   PVLPSAHPKPSKANRGRGGASKRTTRGPAPAPTRENFDALQKEMEAMSTQMRTMEEMYNE 61
           P L  AHPKP KANRGRGGAS+RTT G APAP+RENFDALQKEMEAM TQM TMEEMYNE
Sbjct: 53  PDLSPAHPKPFKANRGRGGASRRTTLGAAPAPSRENFDALQKEMEAMRTQMLTMEEMYNE 112

Query: 62  MVQAAGARSQSEDRVARGEVHERRGLHLGPVEEECPGGDEDEEYTHQRGDLREHLNRKRS 121
           MVQA GA S+SEDR AR E                            RGDLR+HL+RKRS
Sbjct: 113 MVQAVGAGSRSEDRAARDE----------------------------RGDLRDHLSRKRS 172

Query: 122 SSLRKGRSPSCSHKSSNQQAE----------------------------------CEKKE 181
           SSLRKGRSPSCSHK+SNQQAE                                  CE K 
Sbjct: 173 SSLRKGRSPSCSHKNSNQQAESSYNPVVPEGVITREEFDQLKSKFDAQVETLKARCEVKG 232

Query: 182 NSFDNGDLGESPFTSDILEAPIPPKFKTPTIKLYDGSKDPKDYVEVFEGLMDFQAATDAI 221
           ++FD+GDLGESPFTSDILEA IP KFKTPT+K YDGSKDPKDYVEVFEGLM FQAATDAI
Sbjct: 233 STFDDGDLGESPFTSDILEALIPSKFKTPTMKPYDGSKDPKDYVEVFEGLMGFQAATDAI 277

[Close]

BLAST of Moc04g27100 vs. ExPASy TrEMBL
Match: A0A6J1DDW5 (uncharacterized protein LOC111019634 OS=Momordica charantia OX=3673 GN=LOC111019634 PE=4 SV=1)

HSP 1 Score: 265.0 bits (676), Expect = 2.9e-67
Identity = 146/207 (70.53%), Postives = 161/207 (77.78%), Query Frame = 0


Query: 48  MSTQMRTMEEMYNEMVQAAGARSQSEDRVARGEVHERRGLHLGPVEEECPGGDEDEEYTH 107
           M TQM TME+MY+EMVQAAGARS+SE+RVAR ++HE+RG HLGPV++  P G EDEEYTH
Sbjct: 1   MRTQMCTMEKMYSEMVQAAGARSRSENRVARNDMHEQRGHHLGPVKKGHPEGGEDEEYTH 60

Query: 108 QRGDLREHLNRKRSSSLRKGRSPSCSHKSSNQQAE------------------------- 167
           QRGDLREHLNRKRSSSLRKG+SPSCSH++SNQQAE                         
Sbjct: 61  QRGDLREHLNRKRSSSLRKGQSPSCSHRNSNQQAESSYNPITPEGVITREEFDQLKSKFD 120

Query: 168 ---------CEKKENSFDNGDLGESPFTSDILEAPIPPKFKTPTIKLYDGSKDPKDYVEV 221
                    CEKKE+SFD+GDLGESPFTSDILEA IP KFKTPT+K YDGSKDPKDYVEV
Sbjct: 121 AQVEALKAKCEKKESSFDDGDLGESPFTSDILEALIPLKFKTPTMKPYDGSKDPKDYVEV 180

[Close]

BLAST of Moc04g27100 vs. ExPASy TrEMBL
Match: A0A6J1DPN4 (uncharacterized protein LOC111023060 OS=Momordica charantia OX=3673 GN=LOC111023060 PE=4 SV=1)

HSP 1 Score: 220.3 bits (560), Expect = 8.2e-54
Identity = 129/210 (61.43%), Postives = 144/210 (68.57%), Query Frame = 0


Query: 45  MEAMSTQMRTMEEMYNEMVQAAGARSQSEDRVARGEVHERRGLHLGPVEEECPGGDEDEE 104
           MEAM TQMRTMEEMYN+MVQ AGARS+S D+V   +VHE+  LH  PV+EE         
Sbjct: 1   MEAMRTQMRTMEEMYNKMVQIAGARSRSGDQVVHEDVHEQGDLHCDPVDEE--------- 60

Query: 105 YTHQRGDLREHLNRKRSSSLRKGRSPSCSHKSSNQQAE---------------------- 164
             H  GDLR+HLNRKR+SS R  R+ +  HK+SNQQAE                      
Sbjct: 61  --HLGGDLRDHLNRKRNSSHRGERTSTYWHKNSNQQAESSYNPIAPEGVITREEFNQLKS 120

Query: 165 ------------CEKKENSFDNGDLGESPFTSDILEAPIPPKFKTPTIKLYDGSKDPKDY 221
                       CEKKE++FD+GDLGESPFTSDILEA IPPKFKTPT+K YDGSKDPKDY
Sbjct: 121 KFDAQVEALKVRCEKKESAFDDGDLGESPFTSDILEASIPPKFKTPTMKSYDGSKDPKDY 180

[Close]

BLAST of Moc04g27100 vs. ExPASy TrEMBL
Match: A0A6J1DM55 (uncharacterized protein LOC111022267 OS=Momordica charantia OX=3673 GN=LOC111022267 PE=4 SV=1)

HSP 1 Score: 189.5 bits (480), Expect = 1.5e-44
Identity = 104/154 (67.53%), Postives = 113/154 (73.38%), Query Frame = 0


Query: 101 EDEEYTHQRGDLREHLNRKRSSSLRKGRSPSCSHKSSNQQAE------------------ 160
           EDEEYTHQRGDLREHLNRKRSSSLRKG+SPS SH++SNQQAE                  
Sbjct: 33  EDEEYTHQRGDLREHLNRKRSSSLRKGQSPSSSHRNSNQQAESSYNPITPKRVITREEFD 92

Query: 161 ----------------CEKKENSFDNGDLGESPFTSDILEAPIPPKFKTPTIKLYDGSKD 220
                           CEKKE+SFD+GDLGE PFT DILEAPI PKFKTPT+K YDGSK+
Sbjct: 93  QLKSKFDAQVEALKANCEKKESSFDDGDLGELPFTLDILEAPIHPKFKTPTMKPYDGSKN 152

[Close]

BLAST of Moc04g27100 vs. ExPASy TrEMBL
Match: A0A6J1DXR9 (uncharacterized protein LOC111025109 OS=Momordica charantia OX=3673 GN=LOC111025109 PE=4 SV=1)

HSP 1 Score: 155.2 bits (391), Expect = 3.2e-34
Identity = 84/135 (62.22%), Postives = 96/135 (71.11%), Query Frame = 0


Query: 117 NRKRSSSLRKGRSPSCSHKSSNQQAE-------------------------------CEK 176
           + KR SSLRKG+SPS SH+SSNQQAE                               CE+
Sbjct: 3   HEKRGSSLRKGQSPSRSHRSSNQQAESSHNPAGIITREEFDQLRGELDAQVEALKAKCEQ 62

Query: 177 KENSFDNGDLGESPFTSDILEAPIPPKFKTPTIKLYDGSKDPKDYVEVFEGLMDFQAATD 221
           K++S ++GDLGE PFTSD+LEAPIPPKFK PT+K YDG+KDPKDYVEVFEGLMDFQAA+D
Sbjct: 63  KDDSLNDGDLGEPPFTSDVLEAPIPPKFKAPTVKPYDGTKDPKDYVEVFEGLMDFQAASD 122

The following BLAST results are available for this feature:

BLAST of Moc04g27100 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022159327.1	2.7e-67	63.24	uncharacterized protein LOC111025738 [Momordica charantia]	[more]
XP_022151719.1	6.0e-67	70.53	uncharacterized protein LOC111019634 [Momordica charantia]	[more]
XP_022156088.1	1.7e-53	61.43	uncharacterized protein LOC111023060 [Momordica charantia]	[more]
XP_022155128.1	3.2e-44	67.53	uncharacterized protein LOC111022267 [Momordica charantia]	[more]
XP_022158652.1	6.7e-34	62.22	uncharacterized protein LOC111025109 [Momordica charantia]	[more]

BLAST of Moc04g27100 vs. NCBI nr


Match Name	E-value	Identity	Description

BLAST of Moc04g27100 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1DZJ1	1.3e-67	63.24	uncharacterized protein LOC111025738 OS=Momordica charantia OX=3673 GN=LOC111025...	[more]
A0A6J1DDW5	2.9e-67	70.53	uncharacterized protein LOC111019634 OS=Momordica charantia OX=3673 GN=LOC111019...	[more]
A0A6J1DPN4	8.2e-54	61.43	uncharacterized protein LOC111023060 OS=Momordica charantia OX=3673 GN=LOC111023...	[more]
A0A6J1DM55	1.5e-44	67.53	uncharacterized protein LOC111022267 OS=Momordica charantia OX=3673 GN=LOC111022...	[more]
A0A6J1DXR9	3.2e-34	62.22	uncharacterized protein LOC111025109 OS=Momordica charantia OX=3673 GN=LOC111025...	[more]

BLAST of Moc04g27100 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (OHB3-1) v2
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	COILS	Coil	Coil	coord: 38..58
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 1..169
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 73..95
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 102..118

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Moc04g27100.1	Moc04g27100.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0016021	integral component of membrane