Moc04g26470 (gene) Bitter gourd (OHB3-1) v2

Overview
NameMoc04g26470
Typegene
OrganismMomordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
Descriptionadenylate isopentenyltransferase-like
Locationchr4: 19329950 .. 19330939 (+)
RNA-Seq ExpressionMoc04g26470
SyntenyMoc04g26470
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGACACTATTTTCACTCCCCATTTCCCATTCTCCCGCCCTCTTTCTCTCTTTCCCCTGCCCCCGCCCCCGCCGCCGCCTCCCCGTCATCTCCTCCGCCGCCCACGACCACAAGATCCTTCTCATCCTCGGCGCCACCGGTTCCGGCAAGTCCTCCCTCTCCCTCGACCTCGCCGCCCGCTTCCCCTCCTCCGAGATCATCAATTCCGACAAAATGCAGCTCTACTCCGCCCTCGACATCACCACCAATAAGATTCCCCTCCCCCTCCGCCGCGCCGTCCCCCACCACCTCCTCGGCGACTTCGACCCTCTCGCCGCCGACCTCTCCGCCGCCGACTTCCGCTCCCTCGCCGCCCGGGCCATCTCCGCCGTCGTCTCCCGGGGGAACCTCCCGCTCGTCGTCGGCGGCTCCAACTCCTTCATCCACGCCCTCGTCGCCCGCGATTTCGATCCGGACCTCGACGTCTTCGCCGGCCCAGGCCGGGTCTCGGCCGAGTTCCACTACCGCTGCTGCTTCATCTGGGTCGACGTGTCGCTTCCGGTTCTGAAACAGTACCTTTGGAGGCGGGTGGACGACATGTTGGACGCCGGCATGGTGAGGGAGTTGGCCGAGTTTTACGACCCGGCGGTGGCCGAGTCGGCGGCGAGAATCGGGGTGAGGAAGGCCATCGGAGTGCCGGAGTTCGAGGAGTATTTCCGGCGGCATCATCCGAAGGGGAGGGAGTACAGAGGAGGGGCGGATCCGCTGCGGGCGGGCGCATATGAGGACGCGGTGAGGAGGATCAAAGAGAACACGTGGCGGCTCACGAAGAGGCAGTTGGGGAAGATCGGACGGCTGAGAGAGGCCGGGTGGGAGCTCCAGAGAGTGGACGCCACGGCGGCGGTTGTGGCGGTGCTGACGTCGGAAGCGCCGGAGAAACGGTCAGAAATATGGAACGCACAGGTGGTGGAACCGAGCATAAAGATTGTGAAGAGGTTCATCGAGGAGTAG

mRNA sequence

ATGACACTATTTTCACTCCCCATTTCCCATTCTCCCGCCCTCTTTCTCTCTTTCCCCTGCCCCCGCCCCCGCCGCCGCCTCCCCGTCATCTCCTCCGCCGCCCACGACCACAAGATCCTTCTCATCCTCGGCGCCACCGGTTCCGGCAAGTCCTCCCTCTCCCTCGACCTCGCCGCCCGCTTCCCCTCCTCCGAGATCATCAATTCCGACAAAATGCAGCTCTACTCCGCCCTCGACATCACCACCAATAAGATTCCCCTCCCCCTCCGCCGCGCCGTCCCCCACCACCTCCTCGGCGACTTCGACCCTCTCGCCGCCGACCTCTCCGCCGCCGACTTCCGCTCCCTCGCCGCCCGGGCCATCTCCGCCGTCGTCTCCCGGGGGAACCTCCCGCTCGTCGTCGGCGGCTCCAACTCCTTCATCCACGCCCTCGTCGCCCGCGATTTCGATCCGGACCTCGACGTCTTCGCCGGCCCAGGCCGGGTCTCGGCCGAGTTCCACTACCGCTGCTGCTTCATCTGGGTCGACGTGTCGCTTCCGGTTCTGAAACAGTACCTTTGGAGGCGGGTGGACGACATGTTGGACGCCGGCATGGTGAGGGAGTTGGCCGAGTTTTACGACCCGGCGGTGGCCGAGTCGGCGGCGAGAATCGGGGTGAGGAAGGCCATCGGAGTGCCGGAGTTCGAGGAGTATTTCCGGCGGCATCATCCGAAGGGGAGGGAGTACAGAGGAGGGGCGGATCCGCTGCGGGCGGGCGCATATGAGGACGCGGTGAGGAGGATCAAAGAGAACACGTGGCGGCTCACGAAGAGGCAGTTGGGGAAGATCGGACGGCTGAGAGAGGCCGGGTGGGAGCTCCAGAGAGTGGACGCCACGGCGGCGGTTGTGGCGGTGCTGACGTCGGAAGCGCCGGAGAAACGGTCAGAAATATGGAACGCACAGGTGGTGGAACCGAGCATAAAGATTGTGAAGAGGTTCATCGAGGAGTAG

Coding sequence (CDS)

ATGACACTATTTTCACTCCCCATTTCCCATTCTCCCGCCCTCTTTCTCTCTTTCCCCTGCCCCCGCCCCCGCCGCCGCCTCCCCGTCATCTCCTCCGCCGCCCACGACCACAAGATCCTTCTCATCCTCGGCGCCACCGGTTCCGGCAAGTCCTCCCTCTCCCTCGACCTCGCCGCCCGCTTCCCCTCCTCCGAGATCATCAATTCCGACAAAATGCAGCTCTACTCCGCCCTCGACATCACCACCAATAAGATTCCCCTCCCCCTCCGCCGCGCCGTCCCCCACCACCTCCTCGGCGACTTCGACCCTCTCGCCGCCGACCTCTCCGCCGCCGACTTCCGCTCCCTCGCCGCCCGGGCCATCTCCGCCGTCGTCTCCCGGGGGAACCTCCCGCTCGTCGTCGGCGGCTCCAACTCCTTCATCCACGCCCTCGTCGCCCGCGATTTCGATCCGGACCTCGACGTCTTCGCCGGCCCAGGCCGGGTCTCGGCCGAGTTCCACTACCGCTGCTGCTTCATCTGGGTCGACGTGTCGCTTCCGGTTCTGAAACAGTACCTTTGGAGGCGGGTGGACGACATGTTGGACGCCGGCATGGTGAGGGAGTTGGCCGAGTTTTACGACCCGGCGGTGGCCGAGTCGGCGGCGAGAATCGGGGTGAGGAAGGCCATCGGAGTGCCGGAGTTCGAGGAGTATTTCCGGCGGCATCATCCGAAGGGGAGGGAGTACAGAGGAGGGGCGGATCCGCTGCGGGCGGGCGCATATGAGGACGCGGTGAGGAGGATCAAAGAGAACACGTGGCGGCTCACGAAGAGGCAGTTGGGGAAGATCGGACGGCTGAGAGAGGCCGGGTGGGAGCTCCAGAGAGTGGACGCCACGGCGGCGGTTGTGGCGGTGCTGACGTCGGAAGCGCCGGAGAAACGGTCAGAAATATGGAACGCACAGGTGGTGGAACCGAGCATAAAGATTGTGAAGAGGTTCATCGAGGAGTAG

Protein sequence

MTLFSLPISHSPALFLSFPCPRPRRRLPVISSAAHDHKILLILGATGSGKSSLSLDLAARFPSSEIINSDKMQLYSALDITTNKIPLPLRRAVPHHLLGDFDPLAADLSAADFRSLAARAISAVVSRGNLPLVVGGSNSFIHALVARDFDPDLDVFAGPGRVSAEFHYRCCFIWVDVSLPVLKQYLWRRVDDMLDAGMVRELAEFYDPAVAESAARIGVRKAIGVPEFEEYFRRHHPKGREYRGGADPLRAGAYEDAVRRIKENTWRLTKRQLGKIGRLREAGWELQRVDATAAVVAVLTSEAPEKRSEIWNAQVVEPSIKIVKRFIEE
Homology
BLAST of Moc04g26470 vs. NCBI nr
Match: XP_022963367.1 (adenylate isopentenyltransferase-like [Cucurbita moschata])

HSP 1 Score: 464.2 bits (1193), Expect = 9.7e-127
Identity = 249/340 (73.24%), Postives = 278/340 (81.76%), Query Frame = 0

Query: 1   MTLFSLPISHSP----------ALFLSFPCPRPRRRLPVISSAAHDHKILLILGATGSGK 60
           MTL SLP SHS            LFLS   P  R + P + S+     +LL+LGATGSGK
Sbjct: 1   MTL-SLPFSHSSLHYHHHHHPFPLFLS---PSRRFQWPRMLSSLPSDNLLLLLGATGSGK 60

Query: 61  SSLSLDLAARFPSSEIINSDKMQLYSALDITTNKIPLPLRRAVPHHLLGDFDPLAADLSA 120
           SSLS+D+AARFP SEIINSDKMQLY  LD+TTNK+P   RR VPHHLLGDFDPLA ++SA
Sbjct: 61  SSLSIDIAARFP-SEIINSDKMQLYRGLDVTTNKLPFSDRRGVPHHLLGDFDPLAGEVSA 120

Query: 121 ADFRSLAARAISAVVSRGNLPLVVGGSNSFIHALVARDFDPDLDVFAGP-GRVSAEFHYR 180
           A FRS A+  IS VVSRGNLP+VVGGSNS+IHALV   FD D DVF GP  RVSAEF YR
Sbjct: 121 AAFRSQASAVISGVVSRGNLPVVVGGSNSYIHALVTAGFDQDYDVFDGPLNRVSAEFRYR 180

Query: 181 CCFIWVDVSLPVLKQYLWRRVDDMLDAGMVRELAEFYDPAVAESAARIGVRKAIGVPEFE 240
           CCF+WVDVS PVLKQYL +RVDDML+AGMV ELAEFYDP +AE+  R+G+RKAIGVPEFE
Sbjct: 181 CCFLWVDVSFPVLKQYLSKRVDDMLEAGMVGELAEFYDPEMAETEPRVGLRKAIGVPEFE 240

Query: 241 EYFRRHHPKGREYRGGADPLRAGAYEDAVRRIKENTWRLTKRQLGKIGRLREAGWELQRV 300
           E+FRR+HPKGREYR G DPLRAGAYE+AVR+IKENTW+LTKRQLGKIGRLR AGW+L+RV
Sbjct: 241 EFFRRYHPKGREYREG-DPLRAGAYEEAVRKIKENTWQLTKRQLGKIGRLRMAGWDLKRV 300

Query: 301 DATAAVVAVLTSEAPEKRSEIWNAQVVEPSIKIVKRFIEE 330
           DATAAVVAVL SEA EKRSEIW AQVVEPS+KIVKRFIEE
Sbjct: 301 DATAAVVAVLASEAAEKRSEIWEAQVVEPSVKIVKRFIEE 334

BLAST of Moc04g26470 vs. NCBI nr
Match: KAG7032515.1 (hypothetical protein SDJN02_06564, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 463.8 bits (1192), Expect = 1.3e-126
Identity = 246/336 (73.21%), Postives = 275/336 (81.85%), Query Frame = 0

Query: 5   SLPISHSP----------ALFLSFPCPRPRRRLPVISSAAHDHKILLILGATGSGKSSLS 64
           SLP SHS            LFLS   P  R + P + S+     +LL+LGATGSGKSSLS
Sbjct: 4   SLPFSHSSLHYHHHHHPFPLFLS---PSRRFQWPRMLSSLPSDNLLLLLGATGSGKSSLS 63

Query: 65  LDLAARFPSSEIINSDKMQLYSALDITTNKIPLPLRRAVPHHLLGDFDPLAADLSAADFR 124
           +D+AARFP SEIINSDKMQLY  LD+TTNK+P   RR VPHHLLGDFDPLA ++SAA FR
Sbjct: 64  IDIAARFP-SEIINSDKMQLYRGLDVTTNKLPFSDRRGVPHHLLGDFDPLAGEVSAAAFR 123

Query: 125 SLAARAISAVVSRGNLPLVVGGSNSFIHALVARDFDPDLDVFAGP-GRVSAEFHYRCCFI 184
           S A+  IS VVSRGNLP+VVGGSNS+IHALV   FD D DVF GP  RVSAEF YRCCF+
Sbjct: 124 SQASAVISGVVSRGNLPVVVGGSNSYIHALVTAGFDQDYDVFDGPLNRVSAEFRYRCCFL 183

Query: 185 WVDVSLPVLKQYLWRRVDDMLDAGMVRELAEFYDPAVAESAARIGVRKAIGVPEFEEYFR 244
           WVDVS PVLKQYL +RVDDML+AGMV ELAEFYDP +AE+  R+G+RKAIGVPEFEE+FR
Sbjct: 184 WVDVSFPVLKQYLSKRVDDMLEAGMVGELAEFYDPEMAETEPRVGLRKAIGVPEFEEFFR 243

Query: 245 RHHPKGREYRGGADPLRAGAYEDAVRRIKENTWRLTKRQLGKIGRLREAGWELQRVDATA 304
           R+HPKGREYR G DPLRAGAYE+AVR+IKENTW+LTKRQLGKIGRLR AGW+L+RVDATA
Sbjct: 244 RYHPKGREYREG-DPLRAGAYEEAVRKIKENTWQLTKRQLGKIGRLRMAGWDLKRVDATA 303

Query: 305 AVVAVLTSEAPEKRSEIWNAQVVEPSIKIVKRFIEE 330
           AVVAVL SEA EKRSEIW AQVVEPS+KIVKRFIEE
Sbjct: 304 AVVAVLASEAAEKRSEIWEAQVVEPSVKIVKRFIEE 334

BLAST of Moc04g26470 vs. NCBI nr
Match: XP_022971768.1 (adenylate isopentenyltransferase-like [Cucurbita maxima])

HSP 1 Score: 460.7 bits (1184), Expect = 1.1e-125
Identity = 247/340 (72.65%), Postives = 277/340 (81.47%), Query Frame = 0

Query: 1   MTLFSLPISHSP----------ALFLSFPCPRPRRRLPVISSAAHDHKILLILGATGSGK 60
           MTL SLP SHS            LFLS   P  R + P + S+     +LL+LGATGSGK
Sbjct: 1   MTL-SLPFSHSSLHYHHHHHPFPLFLS---PSRRFQWPRMLSSLPSDNLLLLLGATGSGK 60

Query: 61  SSLSLDLAARFPSSEIINSDKMQLYSALDITTNKIPLPLRRAVPHHLLGDFDPLAADLSA 120
           SSLS+D+AARFP SEIINSDKMQLY  LD+TTNK+P   RR VPHHLLGDFDPLA ++SA
Sbjct: 61  SSLSIDIAARFP-SEIINSDKMQLYRGLDVTTNKLPFSDRRGVPHHLLGDFDPLAGEVSA 120

Query: 121 ADFRSLAARAISAVVSRGNLPLVVGGSNSFIHALVARDFDPDLDVFAGP-GRVSAEFHYR 180
           A FRS A+  IS VVSRGNLP+VVGGSNS+IHALV    D D DVF GP  RVSAEF YR
Sbjct: 121 AAFRSQASAVISGVVSRGNLPVVVGGSNSYIHALVTAGLDQDYDVFDGPLNRVSAEFRYR 180

Query: 181 CCFIWVDVSLPVLKQYLWRRVDDMLDAGMVRELAEFYDPAVAESAARIGVRKAIGVPEFE 240
           CCF+WVDVS PVLKQYL +RVDDML+AGMV ELAEFYDP +AE+  R+G+RKAIGVPEFE
Sbjct: 181 CCFLWVDVSFPVLKQYLSKRVDDMLEAGMVGELAEFYDPEMAETEPRVGLRKAIGVPEFE 240

Query: 241 EYFRRHHPKGREYRGGADPLRAGAYEDAVRRIKENTWRLTKRQLGKIGRLREAGWELQRV 300
           E+FRR+HPKGREYR G DPLR+GAYE+AVR+IKENTW+LTKRQLGKIGRLR AGW+L+RV
Sbjct: 241 EFFRRYHPKGREYREG-DPLRSGAYEEAVRKIKENTWQLTKRQLGKIGRLRMAGWDLKRV 300

Query: 301 DATAAVVAVLTSEAPEKRSEIWNAQVVEPSIKIVKRFIEE 330
           DATAAVVAVL SEA EKRSEIW AQVVEPS+KIVKRFIEE
Sbjct: 301 DATAAVVAVLASEAAEKRSEIWEAQVVEPSVKIVKRFIEE 334

BLAST of Moc04g26470 vs. NCBI nr
Match: XP_023515564.1 (adenylate isopentenyltransferase-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 460.7 bits (1184), Expect = 1.1e-125
Identity = 247/340 (72.65%), Postives = 277/340 (81.47%), Query Frame = 0

Query: 1   MTLFSLPISHSP----------ALFLSFPCPRPRRRLPVISSAAHDHKILLILGATGSGK 60
           MTL SLP SHS            LFLS   P  R + P + S+     +LL+LGATGSGK
Sbjct: 1   MTL-SLPFSHSSLHYHHHHHPFPLFLS---PSRRFQWPRMLSSLPSDNLLLLLGATGSGK 60

Query: 61  SSLSLDLAARFPSSEIINSDKMQLYSALDITTNKIPLPLRRAVPHHLLGDFDPLAADLSA 120
           SSLS+D+AARFP SEIINSDKMQLY  LD+TTNK+P   RR VPHHLLGDFDPLA ++SA
Sbjct: 61  SSLSIDIAARFP-SEIINSDKMQLYRGLDVTTNKLPFSDRRGVPHHLLGDFDPLAGEVSA 120

Query: 121 ADFRSLAARAISAVVSRGNLPLVVGGSNSFIHALVARDFDPDLDVFAGP-GRVSAEFHYR 180
           A FRS A+  IS VVSRGNLP+VVGGSNS+IHALV    D D DVF GP  R+SAEF YR
Sbjct: 121 AAFRSQASAVISGVVSRGNLPVVVGGSNSYIHALVTAGLDQDYDVFDGPLNRLSAEFRYR 180

Query: 181 CCFIWVDVSLPVLKQYLWRRVDDMLDAGMVRELAEFYDPAVAESAARIGVRKAIGVPEFE 240
           CCF+WVDVS PVLKQYL +RVDDML+AGMV ELAEFYDP +AE+  R+G+RKAIGVPEFE
Sbjct: 181 CCFLWVDVSFPVLKQYLSKRVDDMLEAGMVGELAEFYDPEMAETEPRVGLRKAIGVPEFE 240

Query: 241 EYFRRHHPKGREYRGGADPLRAGAYEDAVRRIKENTWRLTKRQLGKIGRLREAGWELQRV 300
           E+FRR+HPKGREYR G DPLRAGAYE+AVR+IKENTW+LTKRQLGKIGRLR AGW+L+RV
Sbjct: 241 EFFRRYHPKGREYREG-DPLRAGAYEEAVRKIKENTWQLTKRQLGKIGRLRMAGWDLKRV 300

Query: 301 DATAAVVAVLTSEAPEKRSEIWNAQVVEPSIKIVKRFIEE 330
           DATAAVVAVL SEA EKRSEIW AQVVEPS+KIVKRFIEE
Sbjct: 301 DATAAVVAVLASEAAEKRSEIWEAQVVEPSVKIVKRFIEE 334

BLAST of Moc04g26470 vs. NCBI nr
Match: XP_022939170.1 (adenylate isopentenyltransferase-like [Cucurbita moschata])

HSP 1 Score: 458.0 bits (1177), Expect = 6.9e-125
Identity = 240/332 (72.29%), Postives = 274/332 (82.53%), Query Frame = 0

Query: 1   MTLFSLPISHSPA---LFLSFPCPRPRRRLP-VISSAAHDHKILLILGATGSGKSSLSLD 60
           MTL SL  SHS         F C   R R P V+SS++  H ++L+LGATGSGKSSLS+D
Sbjct: 43  MTL-SLSFSHSSLHSHFNAPFSCSSRRPRSPLVLSSSSPSHNLILLLGATGSGKSSLSID 102

Query: 61  LAARFPSSEIINSDKMQLYSALDITTNKIPLPLRRAVPHHLLGDFDPLAADLSAADFRSL 120
           LA+RFP SEIINSDKMQLY+ LDITTNK+PL  RRAVPHHLLGDFDPLA ++SA+ FRS 
Sbjct: 103 LASRFP-SEIINSDKMQLYTGLDITTNKLPLSDRRAVPHHLLGDFDPLAGEVSASAFRSH 162

Query: 121 AARAISAVVSRGNLPLVVGGSNSFIHALVARDFDPDLDVF-AGPGRVSAEFHYRCCFIWV 180
           AA  IS V+SRGNLPLVVGGSNSFIHALV  DFDP  DVF     RVS EF YRCCF+WV
Sbjct: 163 AAAVISGVISRGNLPLVVGGSNSFIHALVTADFDPSHDVFGCSRNRVSTEFRYRCCFLWV 222

Query: 181 DVSLPVLKQYLWRRVDDMLDAGMVRELAEFYDPAVAESAARIGVRKAIGVPEFEEYFRRH 240
           DVS PVLK+YL +RVD+ML+AGMVRELAEFYDP +A+S  R+G+RKAIGV EFEE+FRRH
Sbjct: 223 DVSFPVLKRYLSKRVDEMLEAGMVRELAEFYDPEIADSETRVGLRKAIGVAEFEEFFRRH 282

Query: 241 HPKGREYRGGADPLRAGAYEDAVRRIKENTWRLTKRQLGKIGRLREAGWELQRVDATAAV 300
           HPKGREY+ G DP+ A AY++AVR+IKENTW+LTKRQLGKIGRLR AGW+L+RVDATAAV
Sbjct: 283 HPKGREYKEGRDPVVASAYKEAVRKIKENTWKLTKRQLGKIGRLRRAGWDLKRVDATAAV 342

Query: 301 VAVLTSEAPEKRSEIWNAQVVEPSIKIVKRFI 328
           VAVL SEA EKRSEIW A+VVE S+KIVKRFI
Sbjct: 343 VAVLGSEAAEKRSEIWEAEVVEASVKIVKRFI 372

BLAST of Moc04g26470 vs. ExPASy Swiss-Prot
Match: Q5GHF7 (Adenylate isopentenyltransferase OS=Humulus lupulus OX=3486 PE=1 SV=1)

HSP 1 Score: 319.7 bits (818), Expect = 3.9e-86
Identity = 172/305 (56.39%), Postives = 217/305 (71.15%), Query Frame = 0

Query: 38  KILLILGATGSGKSSLSLDLAARFPSSEIINSDKMQLYSALDITTNKIPLPLRRAVPHHL 97
           K+L+++GATG+GKS LS+DLAA FP  E+INSDKMQ+Y  LDITTNKI +P R  VPHHL
Sbjct: 31  KLLVLMGATGTGKSRLSIDLAAHFP-LEVINSDKMQVYKGLDITTNKISVPDRGGVPHHL 90

Query: 98  LGDFDPLAADLSAADFRSLAARAISAVVSRGNLPLVVGGSNSFIHALVARDFDPDLDVFA 157
           LG+ DP   +L+ ADFRSLA +A+S +  R  LP++VGGSNSFIHAL+   FD      +
Sbjct: 91  LGEVDPARGELTPADFRSLAGKAVSEITGRRKLPVLVGGSNSFIHALLVDRFDS-----S 150

Query: 158 GPG--------RVSAEFHYRCCFIWVDVSLPVLKQYLWRRVDDMLDAGMVRELAEFYDPA 217
           GPG         VS+E  Y CCF+WVDVS+ VL  YL +RVDDML+ GM  ELAEFY P 
Sbjct: 151 GPGVFEEGSHSVVSSELRYDCCFLWVDVSVKVLTDYLAKRVDDMLELGMFDELAEFYSPE 210

Query: 218 ----VAESAARIGVRKAIGVPEFEEYFRRHHPKGREYRG-GADPLRAGAYEDAVRRIKEN 277
                 +SA R G+RKAIGVPEF+ YF +  P   E    G D +R GA+E+AVR IKEN
Sbjct: 211 DEDHDEDSATRTGLRKAIGVPEFDRYFEKFRPGDVEGEDPGRDRVRRGAFEEAVRAIKEN 270

Query: 278 TWRLTKRQLGKIGRLREAGWELQRVDATAAVVAVLTSEAPEKRSEIWNAQVVEPSIKIVK 330
           T  L KRQ+GKI RL+ AGW+L+R+DAT +  A +TS++ EK +EIW  QV+EPS+KIV 
Sbjct: 271 TCHLAKRQIGKILRLKGAGWDLRRLDATESFRAAMTSDSGEKCTEIWEKQVLEPSVKIVS 329

BLAST of Moc04g26470 vs. ExPASy Swiss-Prot
Match: Q94ID3 (Adenylate isopentenyltransferase 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=IPT1 PE=1 SV=2)

HSP 1 Score: 312.8 bits (800), Expect = 4.7e-84
Identity = 168/329 (51.06%), Postives = 225/329 (68.39%), Query Frame = 0

Query: 9   SHSPALFLSFPCPRPRRRLPVISSAAHDH--------KILLILGATGSGKSSLSLDLAAR 68
           S S +  LSF   R R+  P++SS   +         K+++ILGATG+GKS LS+DLA R
Sbjct: 30  SSSSSSLLSF-TKRRRKHQPLVSSIRMEQSRSRNRKDKVVVILGATGAGKSRLSVDLATR 89

Query: 69  FPSSEIINSDKMQLYSALDITTNKIPLPLRRAVPHHLLGDFDPLAADLSAADFRSLAARA 128
           FP SEIINSDK+Q+Y  L+ITTN+I L  RR VPHHLLG  +P   +L+A +FRS A+  
Sbjct: 90  FP-SEIINSDKIQVYEGLEITTNQITLQDRRGVPHHLLGVINPEHGELTAGEFRSAASNV 149

Query: 129 ISAVVSRGNLPLVVGGSNSFIHALVARDFDPDLDVF-AGPGRVSAEFHYRCCFIWVDVSL 188
           +  + SR  +P++ GGSNSF+HAL+A+ FDP  D F +G   +S++  Y CCFIWVDVS 
Sbjct: 150 VKEITSRQKVPIIAGGSNSFVHALLAQRFDPKFDPFSSGSCLISSDLRYECCFIWVDVSE 209

Query: 189 PVLKQYLWRRVDDMLDAGMVRELAEFYDPAVAESAARIGVRKAIGVPEFEEYFRRHHPKG 248
            VL +YL RRVD+M+D+GM  EL+ FYDP  +    R G+RKAIGVPEF+ YF+ + P+ 
Sbjct: 210 TVLYEYLLRRVDEMMDSGMFEELSRFYDPVKSGLETRFGIRKAIGVPEFDGYFKEYPPEK 269

Query: 249 REYRGGADPLRAGAYEDAVRRIKENTWRLTKRQLGKIGRLREAGWELQRVDATAAVVAVL 308
           +  +   D LR  AY+ AV  IK NTW L KRQ+ KI  L++AGWE++RVDATA+  AV+
Sbjct: 270 KMIKW--DALRKAAYDKAVDDIKRNTWTLAKRQVKKIEMLKDAGWEIERVDATASFKAVM 329

Query: 309 TSEAPEKR-SEIWNAQVVEPSIKIVKRFI 328
              + EK+  E W  QV+EPS+KIVKR +
Sbjct: 330 MKSSSEKKWRENWEEQVLEPSVKIVKRHL 354

BLAST of Moc04g26470 vs. ExPASy Swiss-Prot
Match: Q9LJL4 (Adenylate isopentenyltransferase 8, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=IPT8 PE=2 SV=1)

HSP 1 Score: 293.9 bits (751), Expect = 2.3e-78
Identity = 155/326 (47.55%), Postives = 220/326 (67.48%), Query Frame = 0

Query: 6   LPISHSPALFLSFPCPRPRRRLP---VISSAAHDHKILLILGATGSGKSSLSLDLAARFP 65
           +PI+ SP L L    P PR  +P   V    ++  K+++I+GATGSGKS LS+DLA RF 
Sbjct: 14  IPIT-SPRLRL----PPPRSVVPMTTVCMEQSYKQKVVVIMGATGSGKSCLSIDLATRF- 73

Query: 66  SSEIINSDKMQLYSALDITTNKIPLPLRRAVPHHLLGDFDPLAADLSAADFRSLAARAIS 125
           S EI+NSDK+Q Y  L +TTN++ +  R  VPHHLLG+  P  ++L+ ++FRSLA+R+IS
Sbjct: 74  SGEIVNSDKIQFYDGLKVTTNQMSILERCGVPHHLLGELPPDDSELTTSEFRSLASRSIS 133

Query: 126 AVVSRGNLPLVVGGSNSFIHALVARDFDPDLDVFAGPGRVSAEFHYRCCFIWVDVSLPVL 185
            + +RGNLP++ GGSNSFIHAL+   FDP    F+    +S+   Y CCF+WVDVS+ VL
Sbjct: 134 EITARGNLPIIAGGSNSFIHALLVDRFDPKTYPFSSETSISSGLRYECCFLWVDVSVSVL 193

Query: 186 KQYLWRRVDDMLDAGMVRELAEFYDPAVAESAARI-GVRKAIGVPEFEEYFRRHHPKGRE 245
            +YL +RVD M+++GM  ELA FYDP  + SA R  G+ K IG+PEF+ YF  + P+ ++
Sbjct: 194 FEYLSKRVDQMMESGMFEELAGFYDPRYSGSAIRAHGIHKTIGIPEFDRYFSLYPPERKQ 253

Query: 246 YRGGADPLRAGAYEDAVRRIKENTWRLTKRQLGKIGRLREAGWELQRVDATAAVVAVLTS 305
                D  R GAY++AV+ IKENTWRL K+Q+ +I +L+ +GW++QR+DAT +       
Sbjct: 254 KMSEWDQARKGAYDEAVQEIKENTWRLAKKQIERIMKLKSSGWDIQRLDATPSF------ 313

Query: 306 EAPEKRSEIWNAQVVEPSIKIVKRFI 328
                  EIW+  V++ SIK+VKRF+
Sbjct: 314 --GRSSREIWDNTVLDESIKVVKRFL 325

BLAST of Moc04g26470 vs. ExPASy Swiss-Prot
Match: Q9C6L1 (Adenylate isopentenyltransferase 6, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=IPT6 PE=2 SV=1)

HSP 1 Score: 285.4 bits (729), Expect = 8.1e-76
Identity = 168/346 (48.55%), Postives = 224/346 (64.74%), Query Frame = 0

Query: 1   MTLFSLPISHS---PALFLSFPCPRPRRRLPVISSAAH------DHKILLILGATGSGKS 60
           MTL S P+SHS   P +   F  PR      V +   H        K++LI G TG+GKS
Sbjct: 5   MTLLSPPLSHSSLLPTVTTKFGSPR-----LVTTCMGHAGRKNIKDKVVLITGTTGTGKS 64

Query: 61  SLSLDLAARFPSSEIINSDKMQLYSALDITTNKIPLPLRRAVPHHLLGDFDPLAADLSAA 120
            LS+DLA RF  +EIINSDKMQ+Y   +I TN IPL  +  VPHHLLG F P   +L+ A
Sbjct: 65  RLSVDLATRFFPAEIINSDKMQIYKGFEIVTNLIPLHEQGGVPHHLLGQFHPQDGELTPA 124

Query: 121 DFRSLAARAISAVVSRGNLPLVVGGSNSFIHALVARDFDPDLDVFAGPG----RVSAEFH 180
           +FRSLA  +IS ++S   LP+VVGGSNSF HAL+A  FDPD+D F+ PG     + ++  
Sbjct: 125 EFRSLATLSISKLISSKKLPIVVGGSNSFNHALLAERFDPDIDPFS-PGSSLSTICSDLR 184

Query: 181 YRCCFIWVDVSLPVLKQYLWRRVDDMLDAGMVRELAEFYDPAVAESAARIGVRKAIGVPE 240
           Y+CC +WVDV  PVL Q+L  RVD M+++G+V +LAE YDP V +S  R+GVRK IGV E
Sbjct: 185 YKCCILWVDVLEPVLFQHLCNRVDQMIESGLVEQLAELYDP-VVDSGRRLGVRKTIGVEE 244

Query: 241 FEEYFRRHHPKGREYRGGADPLRAGAYEDAVRRIKENTWRLTKRQLGKIGRLREAGWELQ 300
           F+ YF R +PK  + +G  D  R  AYE+ V+ +KE T RL K+Q  KI +L   GWE++
Sbjct: 245 FDRYF-RVYPKEMD-KGIWDLARKAAYEETVKGMKERTCRLVKKQKEKIMKLIRGGWEIK 304

Query: 301 RVDATAAVVAVL---TSEAPEKRS-EIWNAQVVEPSIKIVKRFIEE 330
           R+DATAA++A L   T++   K   EIW   +V+ S++IVK+F+ E
Sbjct: 305 RLDATAAIMAELNQSTAKGEGKNGREIWEKHIVDESVEIVKKFLLE 341

BLAST of Moc04g26470 vs. ExPASy Swiss-Prot
Match: Q9SB60 (Adenylate isopentenyltransferase 4 OS=Arabidopsis thaliana OX=3702 GN=IPT4 PE=1 SV=1)

HSP 1 Score: 263.5 bits (672), Expect = 3.3e-69
Identity = 145/298 (48.66%), Postives = 203/298 (68.12%), Query Frame = 0

Query: 38  KILLILGATGSGKSSLSLDLAARFPSSEIINSDKMQLYSALDITTNKIPLPLRRAVPHHL 97
           K+++I+GATGSGKSSLS+DLA  F  +EIINSDKMQ Y  L ITTN+  +  RR VPHHL
Sbjct: 6   KMVVIMGATGSGKSSLSVDLALHF-KAEIINSDKMQFYDGLKITTNQSTIEDRRGVPHHL 65

Query: 98  LGDFDPLAADLSAADFRSLAARAISAVVSRGNLPLVVGGSNSFIHALVARDFDPDLDVFA 157
           LG+ +P A +++AA+FR +AA AIS +  R  LP++ GGSNS+IHAL+A+ +DP+   F+
Sbjct: 66  LGELNPEAGEVTAAEFRVMAAEAISEITQRKKLPILAGGSNSYIHALLAKSYDPENYPFS 125

Query: 158 G-PGRVSAEFHYRCCFIWVDVSLPVLKQYLWRRVDDMLDAGMVRELAEFYDPAVAESAAR 217
              G + +E  Y CCFIW+DV   VL +YL  R+D M+ +GM  E+AEF+    A     
Sbjct: 126 DHKGSICSELKYDCCFIWIDVDQSVLFEYLSLRLDLMMKSGMFEEIAEFHRSKKAPKEP- 185

Query: 218 IGVRKAIGVPEFEEYFRRHHPKGREYRGGADPLRAGAYEDAVRRIKENTWRLTKRQLGKI 277
           +G+ KAIGV EF++Y + +  K        DP+R  AYE AVR IKENT++LTK Q+ KI
Sbjct: 186 LGIWKAIGVQEFDDYLKMY--KWDNDMDKWDPMRKEAYEKAVRAIKENTFQLTKDQITKI 245

Query: 278 GRLREAGWELQRVDATA----AVVAVLTSE-APEKRSEIWNAQVVEPSIKIVKRFIEE 330
            +LR AGW++++VDATA    A+ A    E   E + +IWN +V+EP +KIV+  +++
Sbjct: 246 NKLRNAGWDIKKVDATASFREAIRAAKEGEGVAEMQRKIWNKEVLEPCVKIVRSHLDQ 299

BLAST of Moc04g26470 vs. ExPASy TrEMBL
Match: A0A6J1HF40 (adenylate isopentenyltransferase-like OS=Cucurbita moschata OX=3662 GN=LOC111463593 PE=4 SV=1)

HSP 1 Score: 464.2 bits (1193), Expect = 4.7e-127
Identity = 249/340 (73.24%), Postives = 278/340 (81.76%), Query Frame = 0

Query: 1   MTLFSLPISHSP----------ALFLSFPCPRPRRRLPVISSAAHDHKILLILGATGSGK 60
           MTL SLP SHS            LFLS   P  R + P + S+     +LL+LGATGSGK
Sbjct: 1   MTL-SLPFSHSSLHYHHHHHPFPLFLS---PSRRFQWPRMLSSLPSDNLLLLLGATGSGK 60

Query: 61  SSLSLDLAARFPSSEIINSDKMQLYSALDITTNKIPLPLRRAVPHHLLGDFDPLAADLSA 120
           SSLS+D+AARFP SEIINSDKMQLY  LD+TTNK+P   RR VPHHLLGDFDPLA ++SA
Sbjct: 61  SSLSIDIAARFP-SEIINSDKMQLYRGLDVTTNKLPFSDRRGVPHHLLGDFDPLAGEVSA 120

Query: 121 ADFRSLAARAISAVVSRGNLPLVVGGSNSFIHALVARDFDPDLDVFAGP-GRVSAEFHYR 180
           A FRS A+  IS VVSRGNLP+VVGGSNS+IHALV   FD D DVF GP  RVSAEF YR
Sbjct: 121 AAFRSQASAVISGVVSRGNLPVVVGGSNSYIHALVTAGFDQDYDVFDGPLNRVSAEFRYR 180

Query: 181 CCFIWVDVSLPVLKQYLWRRVDDMLDAGMVRELAEFYDPAVAESAARIGVRKAIGVPEFE 240
           CCF+WVDVS PVLKQYL +RVDDML+AGMV ELAEFYDP +AE+  R+G+RKAIGVPEFE
Sbjct: 181 CCFLWVDVSFPVLKQYLSKRVDDMLEAGMVGELAEFYDPEMAETEPRVGLRKAIGVPEFE 240

Query: 241 EYFRRHHPKGREYRGGADPLRAGAYEDAVRRIKENTWRLTKRQLGKIGRLREAGWELQRV 300
           E+FRR+HPKGREYR G DPLRAGAYE+AVR+IKENTW+LTKRQLGKIGRLR AGW+L+RV
Sbjct: 241 EFFRRYHPKGREYREG-DPLRAGAYEEAVRKIKENTWQLTKRQLGKIGRLRMAGWDLKRV 300

Query: 301 DATAAVVAVLTSEAPEKRSEIWNAQVVEPSIKIVKRFIEE 330
           DATAAVVAVL SEA EKRSEIW AQVVEPS+KIVKRFIEE
Sbjct: 301 DATAAVVAVLASEAAEKRSEIWEAQVVEPSVKIVKRFIEE 334

BLAST of Moc04g26470 vs. ExPASy TrEMBL
Match: A0A6J1I7X6 (adenylate isopentenyltransferase-like OS=Cucurbita maxima OX=3661 GN=LOC111470452 PE=3 SV=1)

HSP 1 Score: 460.7 bits (1184), Expect = 5.2e-126
Identity = 247/340 (72.65%), Postives = 277/340 (81.47%), Query Frame = 0

Query: 1   MTLFSLPISHSP----------ALFLSFPCPRPRRRLPVISSAAHDHKILLILGATGSGK 60
           MTL SLP SHS            LFLS   P  R + P + S+     +LL+LGATGSGK
Sbjct: 1   MTL-SLPFSHSSLHYHHHHHPFPLFLS---PSRRFQWPRMLSSLPSDNLLLLLGATGSGK 60

Query: 61  SSLSLDLAARFPSSEIINSDKMQLYSALDITTNKIPLPLRRAVPHHLLGDFDPLAADLSA 120
           SSLS+D+AARFP SEIINSDKMQLY  LD+TTNK+P   RR VPHHLLGDFDPLA ++SA
Sbjct: 61  SSLSIDIAARFP-SEIINSDKMQLYRGLDVTTNKLPFSDRRGVPHHLLGDFDPLAGEVSA 120

Query: 121 ADFRSLAARAISAVVSRGNLPLVVGGSNSFIHALVARDFDPDLDVFAGP-GRVSAEFHYR 180
           A FRS A+  IS VVSRGNLP+VVGGSNS+IHALV    D D DVF GP  RVSAEF YR
Sbjct: 121 AAFRSQASAVISGVVSRGNLPVVVGGSNSYIHALVTAGLDQDYDVFDGPLNRVSAEFRYR 180

Query: 181 CCFIWVDVSLPVLKQYLWRRVDDMLDAGMVRELAEFYDPAVAESAARIGVRKAIGVPEFE 240
           CCF+WVDVS PVLKQYL +RVDDML+AGMV ELAEFYDP +AE+  R+G+RKAIGVPEFE
Sbjct: 181 CCFLWVDVSFPVLKQYLSKRVDDMLEAGMVGELAEFYDPEMAETEPRVGLRKAIGVPEFE 240

Query: 241 EYFRRHHPKGREYRGGADPLRAGAYEDAVRRIKENTWRLTKRQLGKIGRLREAGWELQRV 300
           E+FRR+HPKGREYR G DPLR+GAYE+AVR+IKENTW+LTKRQLGKIGRLR AGW+L+RV
Sbjct: 241 EFFRRYHPKGREYREG-DPLRSGAYEEAVRKIKENTWQLTKRQLGKIGRLRMAGWDLKRV 300

Query: 301 DATAAVVAVLTSEAPEKRSEIWNAQVVEPSIKIVKRFIEE 330
           DATAAVVAVL SEA EKRSEIW AQVVEPS+KIVKRFIEE
Sbjct: 301 DATAAVVAVLASEAAEKRSEIWEAQVVEPSVKIVKRFIEE 334

BLAST of Moc04g26470 vs. ExPASy TrEMBL
Match: A0A6J1FLX4 (adenylate isopentenyltransferase-like OS=Cucurbita moschata OX=3662 GN=LOC111445165 PE=4 SV=1)

HSP 1 Score: 458.0 bits (1177), Expect = 3.4e-125
Identity = 240/332 (72.29%), Postives = 274/332 (82.53%), Query Frame = 0

Query: 1   MTLFSLPISHSPA---LFLSFPCPRPRRRLP-VISSAAHDHKILLILGATGSGKSSLSLD 60
           MTL SL  SHS         F C   R R P V+SS++  H ++L+LGATGSGKSSLS+D
Sbjct: 43  MTL-SLSFSHSSLHSHFNAPFSCSSRRPRSPLVLSSSSPSHNLILLLGATGSGKSSLSID 102

Query: 61  LAARFPSSEIINSDKMQLYSALDITTNKIPLPLRRAVPHHLLGDFDPLAADLSAADFRSL 120
           LA+RFP SEIINSDKMQLY+ LDITTNK+PL  RRAVPHHLLGDFDPLA ++SA+ FRS 
Sbjct: 103 LASRFP-SEIINSDKMQLYTGLDITTNKLPLSDRRAVPHHLLGDFDPLAGEVSASAFRSH 162

Query: 121 AARAISAVVSRGNLPLVVGGSNSFIHALVARDFDPDLDVF-AGPGRVSAEFHYRCCFIWV 180
           AA  IS V+SRGNLPLVVGGSNSFIHALV  DFDP  DVF     RVS EF YRCCF+WV
Sbjct: 163 AAAVISGVISRGNLPLVVGGSNSFIHALVTADFDPSHDVFGCSRNRVSTEFRYRCCFLWV 222

Query: 181 DVSLPVLKQYLWRRVDDMLDAGMVRELAEFYDPAVAESAARIGVRKAIGVPEFEEYFRRH 240
           DVS PVLK+YL +RVD+ML+AGMVRELAEFYDP +A+S  R+G+RKAIGV EFEE+FRRH
Sbjct: 223 DVSFPVLKRYLSKRVDEMLEAGMVRELAEFYDPEIADSETRVGLRKAIGVAEFEEFFRRH 282

Query: 241 HPKGREYRGGADPLRAGAYEDAVRRIKENTWRLTKRQLGKIGRLREAGWELQRVDATAAV 300
           HPKGREY+ G DP+ A AY++AVR+IKENTW+LTKRQLGKIGRLR AGW+L+RVDATAAV
Sbjct: 283 HPKGREYKEGRDPVVASAYKEAVRKIKENTWKLTKRQLGKIGRLRRAGWDLKRVDATAAV 342

Query: 301 VAVLTSEAPEKRSEIWNAQVVEPSIKIVKRFI 328
           VAVL SEA EKRSEIW A+VVE S+KIVKRFI
Sbjct: 343 VAVLGSEAAEKRSEIWEAEVVEASVKIVKRFI 372

BLAST of Moc04g26470 vs. ExPASy TrEMBL
Match: A0A5D3CF13 (Adenylate isopentenyltransferase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold383G00180 PE=4 SV=1)

HSP 1 Score: 456.8 bits (1174), Expect = 7.5e-125
Identity = 241/330 (73.03%), Postives = 273/330 (82.73%), Query Frame = 0

Query: 1   MTLFSLPISHSPALFLSFPCPRPRRRLPVISSAAHDHKILLILGATGSGKSSLSLDLAAR 60
           MTL SLP SH      SFP  R R R P++ S+A    +LL+LGATGSGKSSLS+DLAAR
Sbjct: 1   MTL-SLPFSH------SFPLSR-RPRCPLVLSSAPSDSLLLLLGATGSGKSSLSIDLAAR 60

Query: 61  FPSSEIINSDKMQLYSALDITTNKIPLPLRRAVPHHLLGDFDPLAADLSAADFRSLAARA 120
           FP SEIINSDKMQLY  LDITTNK+PL  RRAVPHHLLGD+DPLA ++S A FRS A+  
Sbjct: 61  FP-SEIINSDKMQLYRGLDITTNKLPLSHRRAVPHHLLGDYDPLAGEVSPAAFRSHASAV 120

Query: 121 ISAVVSRGNLPLVVGGSNSFIHALVARDFDPDLDVFAGP-GRVSAEFHYRCCFIWVDVSL 180
           IS V+SRGNLPLVVGGSNSFIHALVA DFD   DVF G   RVSAEF YRCCF+WVDVS 
Sbjct: 121 ISGVISRGNLPLVVGGSNSFIHALVAADFDDTQDVFCGSRNRVSAEFRYRCCFLWVDVSF 180

Query: 181 PVLKQYLWRRVDDMLDAGMVRELAEFYDPAVAESAARIGVRKAIGVPEFEEYFRRHHPKG 240
           PVLK+YL +RVD+MLD GM+ ELA+FYDP +A+   R+G+RKAIGVPEFEEYFRRHHPKG
Sbjct: 181 PVLKRYLSKRVDEMLDGGMLEELAQFYDPEMADLETRVGLRKAIGVPEFEEYFRRHHPKG 240

Query: 241 REYRGGADPLRAGAYEDAVRRIKENTWRLTKRQLGKIGRLREAGWELQRVDATAAVVAVL 300
           R+YR G DPL+A A+E+AVR+IKENTW+LTKRQL KIGRLR AGW+L+RVDATAAVVAVL
Sbjct: 241 RDYREG-DPLQASAFEEAVRKIKENTWQLTKRQLWKIGRLRRAGWDLKRVDATAAVVAVL 300

Query: 301 TSEAPEKRSEIWNAQVVEPSIKIVKRFIEE 330
            SEA EKRSEIW  QVVEPS+KIVK FI+E
Sbjct: 301 ASEAAEKRSEIWETQVVEPSVKIVKGFIQE 320

BLAST of Moc04g26470 vs. ExPASy TrEMBL
Match: A0A1S3BD73 (adenylate isopentenyltransferase-like OS=Cucumis melo OX=3656 GN=LOC103488599 PE=4 SV=1)

HSP 1 Score: 456.8 bits (1174), Expect = 7.5e-125
Identity = 241/330 (73.03%), Postives = 273/330 (82.73%), Query Frame = 0

Query: 1   MTLFSLPISHSPALFLSFPCPRPRRRLPVISSAAHDHKILLILGATGSGKSSLSLDLAAR 60
           MTL SLP SH      SFP  R R R P++ S+A    +LL+LGATGSGKSSLS+DLAAR
Sbjct: 1   MTL-SLPFSH------SFPLSR-RPRCPLVLSSAPSDSLLLLLGATGSGKSSLSIDLAAR 60

Query: 61  FPSSEIINSDKMQLYSALDITTNKIPLPLRRAVPHHLLGDFDPLAADLSAADFRSLAARA 120
           FP SEIINSDKMQLY  LDITTNK+PL  RRAVPHHLLGD+DPLA ++S A FRS A+  
Sbjct: 61  FP-SEIINSDKMQLYRGLDITTNKLPLSHRRAVPHHLLGDYDPLAGEVSPAAFRSHASAV 120

Query: 121 ISAVVSRGNLPLVVGGSNSFIHALVARDFDPDLDVFAGP-GRVSAEFHYRCCFIWVDVSL 180
           IS V+SRGNLPLVVGGSNSFIHALVA DFD   DVF G   RVSAEF YRCCF+WVDVS 
Sbjct: 121 ISGVISRGNLPLVVGGSNSFIHALVAADFDDTQDVFCGSRNRVSAEFRYRCCFLWVDVSF 180

Query: 181 PVLKQYLWRRVDDMLDAGMVRELAEFYDPAVAESAARIGVRKAIGVPEFEEYFRRHHPKG 240
           PVLK+YL +RVD+MLD GM+ ELA+FYDP +A+   R+G+RKAIGVPEFEEYFRRHHPKG
Sbjct: 181 PVLKRYLSKRVDEMLDGGMLEELAQFYDPEMADLETRVGLRKAIGVPEFEEYFRRHHPKG 240

Query: 241 REYRGGADPLRAGAYEDAVRRIKENTWRLTKRQLGKIGRLREAGWELQRVDATAAVVAVL 300
           R+YR G DPL+A A+E+AVR+IKENTW+LTKRQL KIGRLR AGW+L+RVDATAAVVAVL
Sbjct: 241 RDYREG-DPLQASAFEEAVRKIKENTWQLTKRQLWKIGRLRRAGWDLKRVDATAAVVAVL 300

Query: 301 TSEAPEKRSEIWNAQVVEPSIKIVKRFIEE 330
            SEA EKRSEIW  QVVEPS+KIVK FI+E
Sbjct: 301 ASEAAEKRSEIWETQVVEPSVKIVKGFIQE 320

BLAST of Moc04g26470 vs. TAIR 10
Match: AT1G68460.1 (isopentenyltransferase 1 )

HSP 1 Score: 312.8 bits (800), Expect = 3.4e-85
Identity = 168/329 (51.06%), Postives = 225/329 (68.39%), Query Frame = 0

Query: 9   SHSPALFLSFPCPRPRRRLPVISSAAHDH--------KILLILGATGSGKSSLSLDLAAR 68
           S S +  LSF   R R+  P++SS   +         K+++ILGATG+GKS LS+DLA R
Sbjct: 30  SSSSSSLLSF-TKRRRKHQPLVSSIRMEQSRSRNRKDKVVVILGATGAGKSRLSVDLATR 89

Query: 69  FPSSEIINSDKMQLYSALDITTNKIPLPLRRAVPHHLLGDFDPLAADLSAADFRSLAARA 128
           FP SEIINSDK+Q+Y  L+ITTN+I L  RR VPHHLLG  +P   +L+A +FRS A+  
Sbjct: 90  FP-SEIINSDKIQVYEGLEITTNQITLQDRRGVPHHLLGVINPEHGELTAGEFRSAASNV 149

Query: 129 ISAVVSRGNLPLVVGGSNSFIHALVARDFDPDLDVF-AGPGRVSAEFHYRCCFIWVDVSL 188
           +  + SR  +P++ GGSNSF+HAL+A+ FDP  D F +G   +S++  Y CCFIWVDVS 
Sbjct: 150 VKEITSRQKVPIIAGGSNSFVHALLAQRFDPKFDPFSSGSCLISSDLRYECCFIWVDVSE 209

Query: 189 PVLKQYLWRRVDDMLDAGMVRELAEFYDPAVAESAARIGVRKAIGVPEFEEYFRRHHPKG 248
            VL +YL RRVD+M+D+GM  EL+ FYDP  +    R G+RKAIGVPEF+ YF+ + P+ 
Sbjct: 210 TVLYEYLLRRVDEMMDSGMFEELSRFYDPVKSGLETRFGIRKAIGVPEFDGYFKEYPPEK 269

Query: 249 REYRGGADPLRAGAYEDAVRRIKENTWRLTKRQLGKIGRLREAGWELQRVDATAAVVAVL 308
           +  +   D LR  AY+ AV  IK NTW L KRQ+ KI  L++AGWE++RVDATA+  AV+
Sbjct: 270 KMIKW--DALRKAAYDKAVDDIKRNTWTLAKRQVKKIEMLKDAGWEIERVDATASFKAVM 329

Query: 309 TSEAPEKR-SEIWNAQVVEPSIKIVKRFI 328
              + EK+  E W  QV+EPS+KIVKR +
Sbjct: 330 MKSSSEKKWRENWEEQVLEPSVKIVKRHL 354

BLAST of Moc04g26470 vs. TAIR 10
Match: AT3G19160.1 (ATP/ADP isopentenyltransferases )

HSP 1 Score: 293.9 bits (751), Expect = 1.6e-79
Identity = 155/326 (47.55%), Postives = 220/326 (67.48%), Query Frame = 0

Query: 6   LPISHSPALFLSFPCPRPRRRLP---VISSAAHDHKILLILGATGSGKSSLSLDLAARFP 65
           +PI+ SP L L    P PR  +P   V    ++  K+++I+GATGSGKS LS+DLA RF 
Sbjct: 14  IPIT-SPRLRL----PPPRSVVPMTTVCMEQSYKQKVVVIMGATGSGKSCLSIDLATRF- 73

Query: 66  SSEIINSDKMQLYSALDITTNKIPLPLRRAVPHHLLGDFDPLAADLSAADFRSLAARAIS 125
           S EI+NSDK+Q Y  L +TTN++ +  R  VPHHLLG+  P  ++L+ ++FRSLA+R+IS
Sbjct: 74  SGEIVNSDKIQFYDGLKVTTNQMSILERCGVPHHLLGELPPDDSELTTSEFRSLASRSIS 133

Query: 126 AVVSRGNLPLVVGGSNSFIHALVARDFDPDLDVFAGPGRVSAEFHYRCCFIWVDVSLPVL 185
            + +RGNLP++ GGSNSFIHAL+   FDP    F+    +S+   Y CCF+WVDVS+ VL
Sbjct: 134 EITARGNLPIIAGGSNSFIHALLVDRFDPKTYPFSSETSISSGLRYECCFLWVDVSVSVL 193

Query: 186 KQYLWRRVDDMLDAGMVRELAEFYDPAVAESAARI-GVRKAIGVPEFEEYFRRHHPKGRE 245
            +YL +RVD M+++GM  ELA FYDP  + SA R  G+ K IG+PEF+ YF  + P+ ++
Sbjct: 194 FEYLSKRVDQMMESGMFEELAGFYDPRYSGSAIRAHGIHKTIGIPEFDRYFSLYPPERKQ 253

Query: 246 YRGGADPLRAGAYEDAVRRIKENTWRLTKRQLGKIGRLREAGWELQRVDATAAVVAVLTS 305
                D  R GAY++AV+ IKENTWRL K+Q+ +I +L+ +GW++QR+DAT +       
Sbjct: 254 KMSEWDQARKGAYDEAVQEIKENTWRLAKKQIERIMKLKSSGWDIQRLDATPSF------ 313

Query: 306 EAPEKRSEIWNAQVVEPSIKIVKRFI 328
                  EIW+  V++ SIK+VKRF+
Sbjct: 314 --GRSSREIWDNTVLDESIKVVKRFL 325

BLAST of Moc04g26470 vs. TAIR 10
Match: AT1G25410.1 (isopentenyltransferase 6 )

HSP 1 Score: 285.4 bits (729), Expect = 5.7e-77
Identity = 168/346 (48.55%), Postives = 224/346 (64.74%), Query Frame = 0

Query: 1   MTLFSLPISHS---PALFLSFPCPRPRRRLPVISSAAH------DHKILLILGATGSGKS 60
           MTL S P+SHS   P +   F  PR      V +   H        K++LI G TG+GKS
Sbjct: 5   MTLLSPPLSHSSLLPTVTTKFGSPR-----LVTTCMGHAGRKNIKDKVVLITGTTGTGKS 64

Query: 61  SLSLDLAARFPSSEIINSDKMQLYSALDITTNKIPLPLRRAVPHHLLGDFDPLAADLSAA 120
            LS+DLA RF  +EIINSDKMQ+Y   +I TN IPL  +  VPHHLLG F P   +L+ A
Sbjct: 65  RLSVDLATRFFPAEIINSDKMQIYKGFEIVTNLIPLHEQGGVPHHLLGQFHPQDGELTPA 124

Query: 121 DFRSLAARAISAVVSRGNLPLVVGGSNSFIHALVARDFDPDLDVFAGPG----RVSAEFH 180
           +FRSLA  +IS ++S   LP+VVGGSNSF HAL+A  FDPD+D F+ PG     + ++  
Sbjct: 125 EFRSLATLSISKLISSKKLPIVVGGSNSFNHALLAERFDPDIDPFS-PGSSLSTICSDLR 184

Query: 181 YRCCFIWVDVSLPVLKQYLWRRVDDMLDAGMVRELAEFYDPAVAESAARIGVRKAIGVPE 240
           Y+CC +WVDV  PVL Q+L  RVD M+++G+V +LAE YDP V +S  R+GVRK IGV E
Sbjct: 185 YKCCILWVDVLEPVLFQHLCNRVDQMIESGLVEQLAELYDP-VVDSGRRLGVRKTIGVEE 244

Query: 241 FEEYFRRHHPKGREYRGGADPLRAGAYEDAVRRIKENTWRLTKRQLGKIGRLREAGWELQ 300
           F+ YF R +PK  + +G  D  R  AYE+ V+ +KE T RL K+Q  KI +L   GWE++
Sbjct: 245 FDRYF-RVYPKEMD-KGIWDLARKAAYEETVKGMKERTCRLVKKQKEKIMKLIRGGWEIK 304

Query: 301 RVDATAAVVAVL---TSEAPEKRS-EIWNAQVVEPSIKIVKRFIEE 330
           R+DATAA++A L   T++   K   EIW   +V+ S++IVK+F+ E
Sbjct: 305 RLDATAAIMAELNQSTAKGEGKNGREIWEKHIVDESVEIVKKFLLE 341

BLAST of Moc04g26470 vs. TAIR 10
Match: AT4G24650.1 (isopentenyltransferase 4 )

HSP 1 Score: 263.5 bits (672), Expect = 2.3e-70
Identity = 145/298 (48.66%), Postives = 203/298 (68.12%), Query Frame = 0

Query: 38  KILLILGATGSGKSSLSLDLAARFPSSEIINSDKMQLYSALDITTNKIPLPLRRAVPHHL 97
           K+++I+GATGSGKSSLS+DLA  F  +EIINSDKMQ Y  L ITTN+  +  RR VPHHL
Sbjct: 6   KMVVIMGATGSGKSSLSVDLALHF-KAEIINSDKMQFYDGLKITTNQSTIEDRRGVPHHL 65

Query: 98  LGDFDPLAADLSAADFRSLAARAISAVVSRGNLPLVVGGSNSFIHALVARDFDPDLDVFA 157
           LG+ +P A +++AA+FR +AA AIS +  R  LP++ GGSNS+IHAL+A+ +DP+   F+
Sbjct: 66  LGELNPEAGEVTAAEFRVMAAEAISEITQRKKLPILAGGSNSYIHALLAKSYDPENYPFS 125

Query: 158 G-PGRVSAEFHYRCCFIWVDVSLPVLKQYLWRRVDDMLDAGMVRELAEFYDPAVAESAAR 217
              G + +E  Y CCFIW+DV   VL +YL  R+D M+ +GM  E+AEF+    A     
Sbjct: 126 DHKGSICSELKYDCCFIWIDVDQSVLFEYLSLRLDLMMKSGMFEEIAEFHRSKKAPKEP- 185

Query: 218 IGVRKAIGVPEFEEYFRRHHPKGREYRGGADPLRAGAYEDAVRRIKENTWRLTKRQLGKI 277
           +G+ KAIGV EF++Y + +  K        DP+R  AYE AVR IKENT++LTK Q+ KI
Sbjct: 186 LGIWKAIGVQEFDDYLKMY--KWDNDMDKWDPMRKEAYEKAVRAIKENTFQLTKDQITKI 245

Query: 278 GRLREAGWELQRVDATA----AVVAVLTSE-APEKRSEIWNAQVVEPSIKIVKRFIEE 330
            +LR AGW++++VDATA    A+ A    E   E + +IWN +V+EP +KIV+  +++
Sbjct: 246 NKLRNAGWDIKKVDATASFREAIRAAKEGEGVAEMQRKIWNKEVLEPCVKIVRSHLDQ 299

BLAST of Moc04g26470 vs. TAIR 10
Match: AT5G19040.1 (isopentenyltransferase 5 )

HSP 1 Score: 206.1 bits (523), Expect = 4.4e-53
Identity = 120/291 (41.24%), Postives = 167/291 (57.39%), Query Frame = 0

Query: 38  KILLILGATGSGKSSLSLDLAARFPSSEIINSDKMQLYSALDITTNKIPLPLRRAVPHHL 97
           K++ ++GATG+GKS L++DLA RFP +EI+NSDK+Q+Y  LDI TNK+       VPHHL
Sbjct: 34  KVVFVMGATGTGKSRLAIDLATRFP-AEIVNSDKIQVYKGLDIVTNKVTPEESLGVPHHL 93

Query: 98  LGDFDPLAADLSAADFRSLAARAISAVVSRGNLPLVVGGSNSFIHALVARDFDPDLDVFA 157
           LG       D +A DF+  A RA+ ++V R  +P++ GGSNS+I ALV            
Sbjct: 94  LGTVHDTYEDFTAEDFQREAIRAVESIVQRDRVPIIAGGSNSYIEALV------------ 153

Query: 158 GPGRVSAEFHYRCCFIWVDVSLPVLKQYLWRRVDDMLDAGMVRELAEFYDPAVAESAARI 217
               V     Y CCF+WVDVS PVL  ++  RVD M+D G+V E+   +DP+ ++ +A  
Sbjct: 154 -NDCVDFRLRYNCCFLWVDVSRPVLHSFVSERVDKMVDMGLVDEVRRIFDPSSSDYSA-- 213

Query: 218 GVRKAIGVPEFEEYFRRHHPKGREYRGGADPLRAGAYEDAVRRIKENTWRLTKRQLGKIG 277
           G+R+AIGVPE +E+ R       E R           E A+ +IKENT  L  RQL KI 
Sbjct: 214 GIRRAIGVPELDEFLR------SEMRNYPAETTERLLETAIEKIKENTCLLACRQLQKIQ 273

Query: 278 RL-REAGWELQRVDATAAVVAVLTSEAPEKRSEIWNAQVVEPSIKIVKRFI 328
           RL ++  W + RVDAT   +        E+  E W+  V  PS   V++F+
Sbjct: 274 RLYKQWKWNMHRVDATEVFL-----RRGEEADEAWDNSVAHPSALAVEKFL 297

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022963367.19.7e-12773.24adenylate isopentenyltransferase-like [Cucurbita moschata][more]
KAG7032515.11.3e-12673.21hypothetical protein SDJN02_06564, partial [Cucurbita argyrosperma subsp. argyro... [more]
XP_022971768.11.1e-12572.65adenylate isopentenyltransferase-like [Cucurbita maxima][more]
XP_023515564.11.1e-12572.65adenylate isopentenyltransferase-like [Cucurbita pepo subsp. pepo][more]
XP_022939170.16.9e-12572.29adenylate isopentenyltransferase-like [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
Q5GHF73.9e-8656.39Adenylate isopentenyltransferase OS=Humulus lupulus OX=3486 PE=1 SV=1[more]
Q94ID34.7e-8451.06Adenylate isopentenyltransferase 1, chloroplastic OS=Arabidopsis thaliana OX=370... [more]
Q9LJL42.3e-7847.55Adenylate isopentenyltransferase 8, chloroplastic OS=Arabidopsis thaliana OX=370... [more]
Q9C6L18.1e-7648.55Adenylate isopentenyltransferase 6, chloroplastic OS=Arabidopsis thaliana OX=370... [more]
Q9SB603.3e-6948.66Adenylate isopentenyltransferase 4 OS=Arabidopsis thaliana OX=3702 GN=IPT4 PE=1 ... [more]
Match NameE-valueIdentityDescription
A0A6J1HF404.7e-12773.24adenylate isopentenyltransferase-like OS=Cucurbita moschata OX=3662 GN=LOC111463... [more]
A0A6J1I7X65.2e-12672.65adenylate isopentenyltransferase-like OS=Cucurbita maxima OX=3661 GN=LOC11147045... [more]
A0A6J1FLX43.4e-12572.29adenylate isopentenyltransferase-like OS=Cucurbita moschata OX=3662 GN=LOC111445... [more]
A0A5D3CF137.5e-12573.03Adenylate isopentenyltransferase-like OS=Cucumis melo var. makuwa OX=1194695 GN=... [more]
A0A1S3BD737.5e-12573.03adenylate isopentenyltransferase-like OS=Cucumis melo OX=3656 GN=LOC103488599 PE... [more]
Match NameE-valueIdentityDescription
AT1G68460.13.4e-8551.06isopentenyltransferase 1 [more]
AT3G19160.11.6e-7947.55ATP/ADP isopentenyltransferases [more]
AT1G25410.15.7e-7748.55isopentenyltransferase 6 [more]
AT4G24650.12.3e-7048.66isopentenyltransferase 4 [more]
AT5G19040.14.4e-5341.24isopentenyltransferase 5 [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (OHB3-1) v2
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 320..329
NoneNo IPR availableGENE3D1.10.287.890Crystal structure of tRNA isopentenylpyrophosphate transferase (bh2366) domaincoord: 179..286
e-value: 1.9E-117
score: 393.3
NoneNo IPR availablePFAMPF01715IPPTcoord: 165..273
e-value: 3.6E-12
score: 46.5
coord: 73..147
e-value: 5.5E-15
score: 55.7
NoneNo IPR availablePANTHERPTHR11088:SF65ADENYLATE ISOPENTENYLTRANSFERASE 4-RELATEDcoord: 32..328
IPR027417P-loop containing nucleoside triphosphate hydrolaseGENE3D3.40.50.300coord: 37..327
e-value: 1.9E-117
score: 393.3
IPR027417P-loop containing nucleoside triphosphate hydrolaseSUPERFAMILY52540P-loop containing nucleoside triphosphate hydrolasescoord: 36..213
IPR039657DimethylallyltransferasePANTHERPTHR11088TRNA DIMETHYLALLYLTRANSFERASEcoord: 32..328

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Moc04g26470.1Moc04g26470.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006400 tRNA modification
cellular_component GO:0005739 mitochondrion
molecular_function GO:0009824 AMP dimethylallyltransferase activity
molecular_function GO:0005524 ATP binding
molecular_function GO:0052381 tRNA dimethylallyltransferase activity